znf521 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	znf521
Entrez Gene ID	101731404
Full Name	zinc finger protein 521
Synonyms	ehzf,evi3
General protein information	Preferred Names zinc finger protein 521 Names zinc finger protein 521
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012965302.3	XP_012820756.1	zinc finger protein 521 isoform X3
XM_012965302.2	XP_012820756.1	zinc finger protein 521 isoform X3
XM_012965300.1	XP_012820754.1	zinc finger protein 521 isoform X2
XM_004915209.2	XP_004915266.1	zinc finger protein 521 isoform X1
XM_012965300.2	XP_012820754.1	zinc finger protein 521 isoform X2
XM_004915209.1	XP_004915266.1	zinc finger protein 521 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ZNF521	NP_001098889.1
Mus musculus (house mouse)	Zfp521	NP_663467.1
Pan troglodytes (chimpanzee)	ZNF521	XP_512068.2
Rattus norvegicus (Norway rat)	Zfp521	NP_001100873.1
Canis lupus familiaris (dog)	ZNF521	XP_547633.2
Gallus gallus (chicken)	ZNF521	XP_004939860.1
Xenopus tropicalis (tropical clawed frog)	znf521	XP_004915266.1
Homo sapiens (human)	ZNF521	NP_056276.1
	LOC709169	XP_002800935.1

Gene Ontology

The following znf521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the znf521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa11599	XM_004915209.2 Latest version!	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa11600	XM_012965300.2 Latest version!	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa37703	XM_012965302.3 Latest version!	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa37703	XM_012965302.2	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa11600	XM_012965300.1	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa11599	XM_004915209.1	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

znf521 ( XM_004915209.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915266.1 Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa11599
Clone ID Related Accession (Same CDS sequence)	XM_004915209.2 , XM_004915209.1
Accession Version	XM_004915209.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG 
CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC 
GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT 
GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC 
CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC 
CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC 
AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT 
AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT 
GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG 
CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC 
ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG 
TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT 
GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG 
CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT 
GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC 
GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA 
GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915266.1
CDS	202..4140
Translation	MSRRKQAKPRSLKDPNCKLEDKSEDGESTDCKKRQEEGDELEEEAVHSCDSCLQVFESLSDITEHKISQCQLTDGADVEDDPTCSWPASSPSSKDQASPIHGEGFDFGEEEGIPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDCSQSGSRMDEWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNDDRAALQCMYCHELFVEETSLLNHMEQIHNSEKKNSCNICSENFHSIEELYSHMDSHQHPESCNPSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEVAPPLTKGRGRKRAVQQTGDVNAPTSKQAKVSYSCIYCSKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHETQDPALIVSTMPAMVYQCNFCSEIFNDLNVLQDHIRSSHGFANPVAKDSNAFFCPHCYMGFLTDTSLEEHIRQVHCDLSNSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRAMSPLSPVTLEQSSLKMMQSFGGTPSRPAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRTETDLQLHVKHNHLENQGKIHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFESKTQNCGTNGASEQIQKEEVELQTLLTNNQESHNSHDGSEEDIDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIIKKKAELIKGNYKCNVCSRTFFSEGGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKLPLHSEEDFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGTATPIQSAGRVQNLQKLFKCASCLKEFRSKQDLVKLDINGLPYGLCASCVNLSKSASPNANVTLSSNRQVISQTDSLTCVEGKNNKTSVLKTRCSSCNVKFESETELQNHIQTIHRELVSENSTTQLKTPQVSPMARISPSQSEEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCAQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_004915209.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915209.2

ORF Insert Sequence:

ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG 
CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC 
GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT 
GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC 
CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC 
CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC 
AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT 
AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT 
GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG 
CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC 
ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG 
TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT 
GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG 
CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT 
GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC 
GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA 
GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf521 ( XM_012965300.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820754.1 Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa11600
Clone ID Related Accession (Same CDS sequence)	XM_012965300.1 , XM_012965300.2
Accession Version	XM_012965300.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 521 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG 
CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC 
GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT 
GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC 
CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC 
CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC 
AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT 
AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT 
GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG 
CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC 
ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAAA CCTACCAGTG CATCAAGTGC 
CAGATGGTTT TTTACAATGA ATGGGATATC CAGGTTCATG TTGCAAACCA TATGATTGAT 
GAAGGCCTGA ACCATGAGTG CAAACTGTGC AGCCAGACAT TTGACTCTCC AGCCAAGCTA 
CAGTGCCATC TGATTGAGCA TAGCTTTGAA GGAATGGGGG GCACATTTAA GTGCCCTGTC 
TGCTTTACAG TTTTTGTACA GGCAAACAAG CTGCAGCAAC ACATATTCTC AGCCCACGGA 
CAGGAGGACA AGATCTATGA TTGTGCCCAG TGTCCGCAGA AGTTCTTCTT TCAAACAGAG 
CTGCAGAACC ACACAATGAC CCAGCACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820754.1
CDS	202..4137
Translation	MSRRKQAKPRSLKDPNCKLEDKSEDGESTDCKKRQEEGDELEEEAVHSCDSCLQVFESLSDITEHKISQCQLTDGADVEDDPTCSWPASSPSSKDQASPIHGEGFDFGEEEGIPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDCSQSGSRMDEWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNDDRAALQCMYCHELFVEETSLLNHMEQIHNSEKKNSCNICSENFHSIEELYSHMDSHQHPESCNPSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEVAPPLTKGRGRKRAVQQTGDVNAPTSKQAKVSYSCIYCSKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHETQDPALIVSTMPAMVYQCNFCSEIFNDLNVLQDHIRSSHGFANPVAKDSNAFFCPHCYMGFLTDTSLEEHIRQVHCDLSNSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRAMSPLSPVTLEQSSLKMMQSFGGTPSRPAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRTETDLQLHVKHNHLENQGKIHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFESKTQNCGTNGASEQIQKEEVELQTLLTNNQESHNSHDGSEEDIDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIIKKKAELIKGNYKCNVCSRTFFSEGGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKLPLHSEEDFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGTATPIQSAGRVQNLQKLFKCASCLKEFRSKQDLVKLDINGLPYGLCASCVNLSKSASPNANVTLSSNRQVISQTDSLTCVEGKNNKTSVLKTRCSSCNVKFESETELQNHIQTIHRELVSENSTTQLKTPQVSPMARISPSQSEEKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCAQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_012965300.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965300.2

ORF Insert Sequence:

ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG 
CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC 
GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT 
GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC 
CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC 
CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC 
AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT 
AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT 
GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG 
CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC 
ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAAA CCTACCAGTG CATCAAGTGC 
CAGATGGTTT TTTACAATGA ATGGGATATC CAGGTTCATG TTGCAAACCA TATGATTGAT 
GAAGGCCTGA ACCATGAGTG CAAACTGTGC AGCCAGACAT TTGACTCTCC AGCCAAGCTA 
CAGTGCCATC TGATTGAGCA TAGCTTTGAA GGAATGGGGG GCACATTTAA GTGCCCTGTC 
TGCTTTACAG TTTTTGTACA GGCAAACAAG CTGCAGCAAC ACATATTCTC AGCCCACGGA 
CAGGAGGACA AGATCTATGA TTGTGCCCAG TGTCCGCAGA AGTTCTTCTT TCAAACAGAG 
CTGCAGAACC ACACAATGAC CCAGCACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf521 ( XM_012965302.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820756.1 Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA.

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CloneID	OXa37703
Clone ID Related Accession (Same CDS sequence)	XM_012965302.3 , XM_012965302.2
Accession Version	XM_012965302.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 521 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965302.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG 
TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT 
GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG 
CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT 
GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC 
GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA 
GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820756.1
CDS	851..4129
Translation	MQVHERNKDCSQSGSRMDEWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNDDRAALQCMYCHELFVEETSLLNHMEQIHNSEKKNSCNICSENFHSIEELYSHMDSHQHPESCNPSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEVAPPLTKGRGRKRAVQQTGDVNAPTSKQAKVSYSCIYCSKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHETQDPALIVSTMPAMVYQCNFCSEIFNDLNVLQDHIRSSHGFANPVAKDSNAFFCPHCYMGFLTDTSLEEHIRQVHCDLSNSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRAMSPLSPVTLEQSSLKMMQSFGGTPSRPAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRTETDLQLHVKHNHLENQGKIHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFESKTQNCGTNGASEQIQKEEVELQTLLTNNQESHNSHDGSEEDIDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIIKKKAELIKGNYKCNVCSRTFFSEGGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKLPLHSEEDFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGTATPIQSAGRVQNLQKLFKCASCLKEFRSKQDLVKLDINGLPYGLCASCVNLSKSASPNANVTLSSNRQVISQTDSLTCVEGKNNKTSVLKTRCSSCNVKFESETELQNHIQTIHRELVSENSTTQLKTPQVSPMARISPSQSEEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCAQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_012965302.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965302.3

ORF Insert Sequence:

ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG 
TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT 
GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG 
CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT 
GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC 
GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA 
GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf521 ( XM_004915209.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915266.1 Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa11599
Clone ID Related Accession (Same CDS sequence)	XM_004915209.2 , XM_004915209.1
Accession Version	XM_004915209.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG 
CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC 
GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT 
GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC 
CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC 
CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC 
AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT 
AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT 
GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG 
CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC 
ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG 
TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT 
GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG 
CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT 
GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC 
GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA 
GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915266.1
CDS	203..4141
Translation	MSRRKQAKPRSLKDPNCKLEDKSEDGESTDCKKRQEEGDELEEEAVHSCDSCLQVFESLSDITEHKISQCQLTDGADVEDDPTCSWPASSPSSKDQASPIHGEGFDFGEEEGIPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDCSQSGSRMDEWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNDDRAALQCMYCHELFVEETSLLNHMEQIHNSEKKNSCNICSENFHSIEELYSHMDSHQHPESCNPSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEVAPPLTKGRGRKRAVQQTGDVNAPTSKQAKVSYSCIYCSKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHETQDPALIVSTMPAMVYQCNFCSEIFNDLNVLQDHIRSSHGFANPVAKDSNAFFCPHCYMGFLTDTSLEEHIRQVHCDLSNSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRAMSPLSPVTLEQSSLKMMQSFGGTPSRPAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRTETDLQLHVKHNHLENQGKIHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFESKTQNCGTNGASEQIQKEEVELQTLLTNNQESHNSHDGSEEDIDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIIKKKAELIKGNYKCNVCSRTFFSEGGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKLPLHSEEDFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGTATPIQSAGRVQNLQKLFKCASCLKEFRSKQDLVKLDINGLPYGLCASCVNLSKSASPNANVTLSSNRQVISQTDSLTCVEGKNNKTSVLKTRCSSCNVKFESETELQNHIQTIHRELVSENSTTQLKTPQVSPMARISPSQSEEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCAQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_004915209.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915209.1

ORF Insert Sequence:

ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG 
CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC 
GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT 
GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC 
CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC 
CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC 
AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT 
AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT 
GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG 
CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC 
ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG 
TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT 
GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG 
CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT 
GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC 
GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA 
GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf521 ( XM_012965300.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820754.1 Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa11600
Clone ID Related Accession (Same CDS sequence)	XM_012965300.1 , XM_012965300.2
Accession Version	XM_012965300.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 521 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG 
CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC 
GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT 
GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC 
CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC 
CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC 
AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT 
AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT 
GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG 
CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC 
ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAAA CCTACCAGTG CATCAAGTGC 
CAGATGGTTT TTTACAATGA ATGGGATATC CAGGTTCATG TTGCAAACCA TATGATTGAT 
GAAGGCCTGA ACCATGAGTG CAAACTGTGC AGCCAGACAT TTGACTCTCC AGCCAAGCTA 
CAGTGCCATC TGATTGAGCA TAGCTTTGAA GGAATGGGGG GCACATTTAA GTGCCCTGTC 
TGCTTTACAG TTTTTGTACA GGCAAACAAG CTGCAGCAAC ACATATTCTC AGCCCACGGA 
CAGGAGGACA AGATCTATGA TTGTGCCCAG TGTCCGCAGA AGTTCTTCTT TCAAACAGAG 
CTGCAGAACC ACACAATGAC CCAGCACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820754.1
CDS	203..4138
Translation	MSRRKQAKPRSLKDPNCKLEDKSEDGESTDCKKRQEEGDELEEEAVHSCDSCLQVFESLSDITEHKISQCQLTDGADVEDDPTCSWPASSPSSKDQASPIHGEGFDFGEEEGIPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDCSQSGSRMDEWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNDDRAALQCMYCHELFVEETSLLNHMEQIHNSEKKNSCNICSENFHSIEELYSHMDSHQHPESCNPSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEVAPPLTKGRGRKRAVQQTGDVNAPTSKQAKVSYSCIYCSKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHETQDPALIVSTMPAMVYQCNFCSEIFNDLNVLQDHIRSSHGFANPVAKDSNAFFCPHCYMGFLTDTSLEEHIRQVHCDLSNSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRAMSPLSPVTLEQSSLKMMQSFGGTPSRPAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRTETDLQLHVKHNHLENQGKIHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFESKTQNCGTNGASEQIQKEEVELQTLLTNNQESHNSHDGSEEDIDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIIKKKAELIKGNYKCNVCSRTFFSEGGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKLPLHSEEDFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGTATPIQSAGRVQNLQKLFKCASCLKEFRSKQDLVKLDINGLPYGLCASCVNLSKSASPNANVTLSSNRQVISQTDSLTCVEGKNNKTSVLKTRCSSCNVKFESETELQNHIQTIHRELVSENSTTQLKTPQVSPMARISPSQSEEKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCAQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_012965300.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965300.1

ORF Insert Sequence:

ATGTCTCGTC GCAAGCAAGC GAAGCCAAGA TCGCTGAAAG ATCCAAACTG TAAACTAGAA 
GATAAATCTG AGGATGGAGA ATCGACAGAT TGCAAGAAGA GGCAGGAGGA AGGAGACGAG 
CTAGAAGAAG AAGCCGTTCA CAGCTGTGAC AGTTGCCTTC AGGTGTTTGA ATCTCTGAGC 
GATATCACTG AGCACAAAAT CAGTCAGTGT CAGCTGACAG ATGGCGCAGA TGTAGAAGAT 
GATCCAACGT GTTCATGGCC TGCTTCATCA CCTTCTAGCA AAGATCAAGC ATCACCAATC 
CACGGAGAAG GGTTTGATTT TGGGGAAGAG GAAGGTATTC CAGGACTGCC TTATCCATGC 
CAGTTCTGTG ACAAATCCTT TAGTCGCCTC AGCTACTTGA AGCATCATGA ACAAAGCCAC 
AGTGACAAGC TACCTTTCAA GTGCACATAT TGTAGTAGAC TGTTTAAACA TAAACGAAGT 
AGGGACCGCC ATATAAAACT GCACACAGGA GACAAAAAAT ATCACTGCAG TGAATGTGAT 
GCAGCATTCT CCAGAAGTGA TCATCTTAAG ATTCACTTAA AGACTCATAC ATCCAACAAG 
CCATATAAAT GTGCGATTTG TAGGCGAGGT TTTCTATCAT CTAGTTCTTT ACATGGTCAC 
ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAAA CCTACCAGTG CATCAAGTGC 
CAGATGGTTT TTTACAATGA ATGGGATATC CAGGTTCATG TTGCAAACCA TATGATTGAT 
GAAGGCCTGA ACCATGAGTG CAAACTGTGC AGCCAGACAT TTGACTCTCC AGCCAAGCTA 
CAGTGCCATC TGATTGAGCA TAGCTTTGAA GGAATGGGGG GCACATTTAA GTGCCCTGTC 
TGCTTTACAG TTTTTGTACA GGCAAACAAG CTGCAGCAAC ACATATTCTC AGCCCACGGA 
CAGGAGGACA AGATCTATGA TTGTGCCCAG TGTCCGCAGA AGTTCTTCTT TCAAACAGAG 
CTGCAGAACC ACACAATGAC CCAGCACAGC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf521 ( XM_012965302.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820756.1 Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA.

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CloneID	OXa37703
Clone ID Related Accession (Same CDS sequence)	XM_012965302.3 , XM_012965302.2
Accession Version	XM_012965302.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	zinc finger protein 521 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG 
TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT 
GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG 
CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT 
GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC 
GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA 
GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820756.1
CDS	861..4139
Translation	MQVHERNKDCSQSGSRMDEWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNDDRAALQCMYCHELFVEETSLLNHMEQIHNSEKKNSCNICSENFHSIEELYSHMDSHQHPESCNPSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEVAPPLTKGRGRKRAVQQTGDVNAPTSKQAKVSYSCIYCSKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHETQDPALIVSTMPAMVYQCNFCSEIFNDLNVLQDHIRSSHGFANPVAKDSNAFFCPHCYMGFLTDTSLEEHIRQVHCDLSNSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRAMSPLSPVTLEQSSLKMMQSFGGTPSRPAGEYICNQCGAKYTSLDGFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRTETDLQLHVKHNHLENQGKIHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFESKTQNCGTNGASEQIQKEEVELQTLLTNNQESHNSHDGSEEDIDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIIKKKAELIKGNYKCNVCSRTFFSEGGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKLPLHSEEDFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGTATPIQSAGRVQNLQKLFKCASCLKEFRSKQDLVKLDINGLPYGLCASCVNLSKSASPNANVTLSSNRQVISQTDSLTCVEGKNNKTSVLKTRCSSCNVKFESETELQNHIQTIHRELVSENSTTQLKTPQVSPMARISPSQSEEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCAQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_012965302.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger protein 521 (znf521), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965302.2

ORF Insert Sequence:

ATGCAAGTGC ATGAAAGAAA CAAAGACTGC TCTCAGTCTG GGTCTAGAAT GGATGAATGG 
AAGATGAAAG ATACTCAGAA ATGCAGTCAG TGTGAAGAAG GTTTTGACTT TCCTGAAGAT 
CTCCAAAAGC ATATAGCTGA ATGTCATCCA GAATGTTCTC CAAATGATGA CCGGGCTGCC 
CTCCAGTGTA TGTATTGCCA TGAATTATTT GTGGAAGAAA CATCGCTGCT AAACCACATG 
GAGCAAATAC ACAATAGTGA AAAAAAGAAT TCTTGTAACA TTTGCTCTGA AAACTTTCAT 
AGCATTGAAG AGCTTTACAG CCATATGGAT AGCCATCAGC ATCCAGAGTC TTGTAATCCC 
AGTAACAGTC CATCTTTAGT TACTGTTGGA TATACGTCGG TTTCAAGCAC CACCCCTGAT 
TCAAATTTAT CTGTAGACAG TTCAACTATG GTAGAAGTTG CTCCCCCATT AACAAAAGGC 
CGTGGCAGGA AAAGGGCTGT TCAGCAGACA GGTGATGTCA ACGCACCGAC AAGTAAGCAA 
GCAAAAGTTT CATACAGCTG TATCTACTGC AGCAAGCAGC TCTTTTCTAG CTTGGCAGTT 
TTACAAATCC ACCTTAAAAC TATGCATTTA GATAAACCCG AGCAGGCTCA CATCTGTCAG 
TATTGTTTAG AAGTTTTACC ATCCCTCTAC AATCTAAACG AACATCTGAA ACAGGTACAC 
GAAACTCAGG ATCCAGCACT AATTGTTTCT ACTATGCCTG CCATGGTGTA TCAGTGTAAC 
TTTTGTTCTG AAATATTTAA TGATCTTAAT GTGCTTCAGG ACCACATAAG AAGTTCACAT 
GGGTTTGCAA ACCCCGTTGC TAAAGACAGC AATGCCTTCT TTTGTCCCCA CTGCTACATG 
GGATTCCTTA CTGATACATC TTTAGAAGAG CACATTCGAC AAGTTCATTG TGATCTTAGC 
AATTCTCGTT TTGGTTCCCC AGTGTTAGGA ACTCCAAAAG AGCCAGTTGT TGAGGTTTAT 
TCTTGTTCAT ACTGTACAAA TTCTCCAATC TTTAACAGCG TCCTTAAACT GAACAAGCAC 
ATTAAAGAGA ATCACAAAAA TATTCCTTTA GCATTAAATT ACATCCATAA TGGAAAAAAA 
TCTAGAGCCA TGAGTCCTTT GTCTCCTGTT ACCTTGGAAC AATCTTCTCT AAAAATGATG 
CAGTCCTTTG GAGGCACTCC TTCAAGACCT GCAGGCGAAT ATATCTGTAA TCAGTGTGGT 
GCCAAATATA CATCCCTCGA CGGCTTTCAG ACTCACTTAA AAACTCATCT TGACACAGTT 
TTGCCAAAGC TAACATGTCC TCAGTGTAAC AAAGAATTTC CAAACCAAGA ATCCCTTTTA 
AAGCATGTTA CTATTCACTT CATGATCACC TCTACATATT ACATCTGTGA AAGCTGTGAC 
AAGCAGTTCA CATCAGTTGA TGACTTACAG AAGCATTTGC TGGACATGCA CACTTTTGTG 
TTTTTTCGGT GCACACTGTG CCAAGAGGTG TTCGACTCTA AGGTGTCCAT TCAGCTTCAC 
TTGGCTGTGA AGCACAGTAA TGAAAAAAAA GTGTACCGTT GCACTTCATG TAACTGGGAC 
TTCCGAACAG AGACTGACTT GCAGCTCCAC GTGAAGCACA ATCACTTGGA AAACCAGGGA 
AAAATCCACA AGTGCATTTT CTGTGGTGAA TCGTTTGGCA CTGAGGTAGA GCTGCAATGT 
CACATAACAA CCCACAGCAA AAAGTACAAT TGTAAATTCT GCAGCAAAGC TTTTCATGCA 
ATTATTTTAC TGGAAAAACA CTTGAGGGAA AAACACTGTG TATTTGAATC GAAAACGCAA 
AACTGTGGGA CCAACGGAGC TTCAGAGCAG ATTCAGAAAG AAGAAGTGGA GCTACAAACG 
CTACTCACAA ACAATCAAGA GTCCCATAAT AGCCATGATG GTAGTGAAGA AGATATTGAT 
ACGTCAGAGC CAATGTATGG CTGTGACATA TGTGGAGCTG CCTATACCAT GGAAACCCTT 
CTGCAAAACC ATCAGCTTCG GGACCATAAT ATCAGGCCAG GAGAGAGTGC AATTATTAAG 
AAGAAAGCTG AACTGATTAA GGGGAACTAT AAATGCAATG TATGTTCGCG TACCTTCTTT 
TCTGAAGGTG GATTGCGAGA ACACATGCAG ACCCATTTGG GACCAGTAAA GCATTACATG 
TGTCCAATTT GTGGAGAGAG ATTTCCTTCT CTTTTAACTC TTACTGAACA CAAAGTTACC 
CACAGTAAGA GCCTGGATAC TGGCAACTGC AGAATTTGTA AACTTCCTCT TCACAGTGAG 
GAGGATTTCT TGGAGCACTG TCAGATGCAC CCTGATCTGC GGAACTCCCT GACAGGATTT 
CGCTGCGTGG TTTGCATGCA GACTGTGACC TCAACTCTCG AACTGAAAAT CCATGGCACG 
TTTCATATGC AGAAAACTGG AACTGCAACT CCTATTCAAT CTGCAGGTCG TGTACAGAAC 
TTACAGAAAT TATTTAAATG TGCCTCATGT CTGAAAGAGT TTCGCTCAAA ACAGGATCTT 
GTAAAGCTTG ATATTAATGG TCTGCCCTAT GGCCTGTGCG CTAGCTGTGT TAATCTTAGC 
AAAAGTGCTA GTCCAAATGC CAATGTGACT TTAAGCTCTA ACAGGCAGGT TATAAGCCAA 
ACTGACAGCC TGACTTGTGT GGAAGGTAAA AATAATAAGA CATCTGTTCT AAAGACTCGA 
TGCTCTAGCT GCAATGTTAA ATTTGAATCA GAAACTGAAC TTCAAAATCA CATTCAGACA 
ATCCACAGAG AGCTTGTCTC AGAAAACAGT ACAACGCAAC TGAAAACTCC ACAAGTTTCA 
CCAATGGCCA GGATAAGTCC TTCTCAGTCT GAAGAGAAGA AAACCTACCA GTGCATCAAG 
TGCCAGATGG TTTTTTACAA TGAATGGGAT ATCCAGGTTC ATGTTGCAAA CCATATGATT 
GATGAAGGCC TGAACCATGA GTGCAAACTG TGCAGCCAGA CATTTGACTC TCCAGCCAAG 
CTACAGTGCC ATCTGATTGA GCATAGCTTT GAAGGAATGG GGGGCACATT TAAGTGCCCT 
GTCTGCTTTA CAGTTTTTGT ACAGGCAAAC AAGCTGCAGC AACACATATT CTCAGCCCAC 
GGACAGGAGG ACAAGATCTA TGATTGTGCC CAGTGTCCGC AGAAGTTCTT CTTTCAAACA 
GAGCTGCAGA ACCACACAAT GACCCAGCAC AGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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znf521 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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