• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

gadd45a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Summary

Gene Symbol gadd45a
Entrez Gene ID 548905
Full Name growth arrest and DNA damage inducible alpha
Synonyms ddit1,gadd,gadd45
General protein information
Preferred Names

growth arrest and DNA damage inducible alpha

Names

growth arrest and DNA damage-inducible protein GADD45 alpha
Gene Type protein-coding
Organism Xenopus tropicalis(tropical clawed frog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_012961068.2 XP_012816522.1 growth arrest and DNA damage-inducible protein GADD45 alpha isoform X1
NM_001016151.3 NP_001016151.1 growth arrest and DNA damage-inducible protein GADD45 alpha
XM_012961068.3 XP_012816522.1 growth arrest and DNA damage-inducible protein GADD45 alpha isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) GADD45A NP_001915.1
Pan troglodytes (chimpanzee) GADD45A XP_513476.2
GADD45A XP_001095413.1
Bos taurus (cattle) GADD45A NP_001029419.1
Mus musculus (house mouse) Gadd45a NP_031862.1
Danio rerio (zebrafish) gadd45ab NP_001002216.1
Danio rerio (zebrafish) gadd45aa NP_956870.1
Gallus gallus (chicken) GADD45A NP_001038143.1
Canis lupus familiaris (dog) GADD45A XP_003639149.1
Rattus norvegicus (Norway rat) Gadd45a NP_077041.2
Xenopus tropicalis (tropical clawed frog) gadd45a NP_001016151.1

Gene Ontology


Bibliography

Related articles in PubMed

Gadd45a is an RNA binding protein and is localized in nuclear speckles.
Sytnikova YA, Kubarenko AV, Sch?fer A, Weber AN, Niehrs C
PloS one6(1)e14500(2011 Jan)

Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.
Barreto G, Sch?fer A, Marhold J, Stach D, Swaminathan SK, Handa V, D?derlein G, Maltry N, Wu W, Lyko F, Niehrs C
Nature445(7128)671-5(2007 Feb)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gadd45a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gadd45a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa21592 NM_001016151.3
Latest version!		 Xenopus tropicalis growth arrest and DNA damage inducible alpha (gadd45a), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
OXa27578 XM_012961068.3
Latest version!		 Xenopus tropicalis growth arrest and DNA damage inducible alpha (gadd45a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
View Old Accession Versions >>
OXa27578 XM_012961068.2 Xenopus tropicalis growth arrest and DNA damage inducible alpha (gadd45a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $104.30
$149.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OXa21592
Clone ID Related Accession (Same CDS sequence) NM_001016151.3
Accession Version NM_001016151.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 474bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-21
Organism Xenopus tropicalis(tropical clawed frog)
Product growth arrest and DNA damage-inducible protein GADD45 alpha
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121532.1. On Aug 13, 2006 this sequence version replaced NM_001016151.2. ##Evidence-Data-START## Transcript exon combination :: BC121532.1, DN096241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028295, SAMN00861989 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
ATGACTCTGG AGGAGCAGCT GTCTGGGGAT CAGAGCATTG GAAAGATGGA GTCAGTGGGA 
AACGCACTGG AGGAAGTGCT CTGCAAGGCC AAACTGCAGA GGACCATCAC TATTGGGGTG 
TACGAAGCTG CAAAACTTCT CAATGTGGAC CCAGACAACG TTGTGCTCTG TTTGTTGGCT 
ACTGACGATA CCTACGATCA GGATATCGCT TTACAGATCC ACTTCACCCT GATCCAGGCA 
TTTTGCTGTG AGAATGACAT CAACATCCTG AGAGTGAGCA ACATGAGCCG GCTAGCTGAG 
ATCCTGGGCG GCTCTGACAA GCCTGGGGAA CCTGCTGATC TGCATTGTAT TCTAATCAAC 
AGCCCACATG AATTACAGGT GAAGGATCCT GCTCTCAAAG AAGTGATTTG TTACTGCAAA 
GAGAGTCGTT ACCTGGACCA GTGGGTGCCT GTGATTAACC TGCCAGAGCG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001016151.1
CDS110..583
Translation

Target ORF information:

RefSeq Version NM_001016151.3
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis growth arrest and DNA damage inducible alpha (gadd45a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001016151.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGACTCTGG AGGAGCAGCT GTCTGGGGAT CAGAGCATTG GAAAGATGGA GTCAGTGGGA 
AACGCACTGG AGGAAGTGCT CTGCAAGGCC AAACTGCAGA GGACCATCAC TATTGGGGTG 
TACGAAGCTG CAAAACTTCT CAATGTGGAC CCAGACAACG TTGTGCTCTG TTTGTTGGCT 
ACTGACGATA CCTACGATCA GGATATCGCT TTACAGATCC ACTTCACCCT GATCCAGGCA 
TTTTGCTGTG AGAATGACAT CAACATCCTG AGAGTGAGCA ACATGAGCCG GCTAGCTGAG 
ATCCTGGGCG GCTCTGACAA GCCTGGGGAA CCTGCTGATC TGCATTGTAT TCTAATCAAC 
AGCCCACATG AATTACAGGT GAAGGATCCT GCTCTCAAAG AAGTGATTTG TTACTGCAAA 
GAGAGTCGTT ACCTGGACCA GTGGGTGCCT GTGATTAACC TGCCAGAGCG ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa27578
Clone ID Related Accession (Same CDS sequence) XM_012961068.2 , XM_012961068.3
Accession Version XM_012961068.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 372bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-09-07
Organism Xenopus tropicalis(tropical clawed frog)
Product growth arrest and DNA damage-inducible protein GADD45 alpha isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
ATGACTCTGG AGGAGCAGCT GTCTGGGGAT CAGAGCATTG GAAAGGACCC AGACAACGTT 
GTGCTCTGTT TGTTGGCTAC TGACGATACC TACGATCAGG ATATCGCTTT ACAGATCCAC 
TTCACCCTGA TCCAGGCATT TTGCTGTGAG AATGACATCA ACATCCTGAG AGTGAGCAAC 
ATGAGCCGGC TAGCTGAGAT CCTGGGCGGC TCTGACAAGC CTGGGGAACC TGCTGATCTG 
CATTGTATTC TAATCAACAG CCCACATGAA TTACAGGTGA AGGATCCTGC TCTCAAAGAA 
GTGATTTGTT ACTGCAAAGA GAGTCGTTAC CTGGACCAGT GGGTGCCTGT GATTAACCTG 
CCAGAGCGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012816522.1
CDS199..570
Translation

Target ORF information:

RefSeq Version XM_012961068.2
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis growth arrest and DNA damage inducible alpha (gadd45a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012961068.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGACTCTGG AGGAGCAGCT GTCTGGGGAT CAGAGCATTG GAAAGGACCC AGACAACGTT 
GTGCTCTGTT TGTTGGCTAC TGACGATACC TACGATCAGG ATATCGCTTT ACAGATCCAC 
TTCACCCTGA TCCAGGCATT TTGCTGTGAG AATGACATCA ACATCCTGAG AGTGAGCAAC 
ATGAGCCGGC TAGCTGAGAT CCTGGGCGGC TCTGACAAGC CTGGGGAACC TGCTGATCTG 
CATTGTATTC TAATCAACAG CCCACATGAA TTACAGGTGA AGGATCCTGC TCTCAAAGAA 
GTGATTTGTT ACTGCAAAGA GAGTCGTTAC CTGGACCAGT GGGTGCCTGT GATTAACCTG 
CCAGAGCGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OXa27578
Clone ID Related Accession (Same CDS sequence) XM_012961068.2 , XM_012961068.3
Accession Version XM_012961068.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 372bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-17
Organism Xenopus tropicalis(tropical clawed frog)
Product growth arrest and DNA damage-inducible protein GADD45 alpha isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
ATGACTCTGG AGGAGCAGCT GTCTGGGGAT CAGAGCATTG GAAAGGACCC AGACAACGTT 
GTGCTCTGTT TGTTGGCTAC TGACGATACC TACGATCAGG ATATCGCTTT ACAGATCCAC 
TTCACCCTGA TCCAGGCATT TTGCTGTGAG AATGACATCA ACATCCTGAG AGTGAGCAAC 
ATGAGCCGGC TAGCTGAGAT CCTGGGCGGC TCTGACAAGC CTGGGGAACC TGCTGATCTG 
CATTGTATTC TAATCAACAG CCCACATGAA TTACAGGTGA AGGATCCTGC TCTCAAAGAA 
GTGATTTGTT ACTGCAAAGA GAGTCGTTAC CTGGACCAGT GGGTGCCTGT GATTAACCTG 
CCAGAGCGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_012816522.1
CDS257..628
Translation

Target ORF information:

RefSeq Version XM_012961068.3
Organism Xenopus tropicalis(tropical clawed frog)
Definition Xenopus tropicalis growth arrest and DNA damage inducible alpha (gadd45a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_012961068.3

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGACTCTGG AGGAGCAGCT GTCTGGGGAT CAGAGCATTG GAAAGGACCC AGACAACGTT 
GTGCTCTGTT TGTTGGCTAC TGACGATACC TACGATCAGG ATATCGCTTT ACAGATCCAC 
TTCACCCTGA TCCAGGCATT TTGCTGTGAG AATGACATCA ACATCCTGAG AGTGAGCAAC 
ATGAGCCGGC TAGCTGAGAT CCTGGGCGGC TCTGACAAGC CTGGGGAACC TGCTGATCTG 
CATTGTATTC TAATCAACAG CCCACATGAA TTACAGGTGA AGGATCCTGC TCTCAAAGAA 
GTGATTTGTT ACTGCAAAGA GAGTCGTTAC CTGGACCAGT GGGTGCCTGT GATTAACCTG 
CCAGAGCGAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gadd45a is an RNA binding protein and is localized in nuclear speckles.
PloS one6(1)e14500(2011 Jan)
Sytnikova YA,Kubarenko AV,Sch?fer A,Weber AN,Niehrs C


book

Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.
Nature445(7128)671-5(2007 Feb)
Barreto G,Sch?fer A,Marhold J,Stach D,Swaminathan SK,Handa V,D?derlein G,Maltry N,Wu W,Lyko F,Niehrs C


book

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P