hmg3 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	hmg3
Entrez Gene ID	542455
Full Name	high mobility group protein 3
Synonyms	GRMZM2G156785,HMG11,HMGc2
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
XM_008679570.2	XP_008677792.1	high mobility group protein 3 isoform X1
NM_001112013.1	NP_001105483.1	high mobility group protein 3
XM_008679571.2	XP_008677793.1	high mobility group protein 3 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The B73 maize genome: complexity, diversity, and dynamics.
Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C, Zhang J, Fulton L, Graves TA, Minx P, Reily AD, Courtney L, Kruchowski SS, Tomlinson C, Strong C, Delehaunty K, Fronick C, Courtney B, Rock SM, Belter E, Du F, Kim K, Abbott RM, Cotton M, Levy A, Marchetto P, Ochoa K, Jackson SM, Gillam B, Chen W, Yan L, Higginbotham J, Cardenas M, Waligorski J, Applebaum E, Phelps L, Falcone J, Kanchi K, Thane T, Scimone A, Thane N, Henke J, Wang T, Ruppert J, Shah N, Rotter K, Hodges J, Ingenthron E, Cordes M, Kohlberg S, Sgro J, Delgado B, Mead K, Chinwalla A, Leonard S, Crouse K, Collura K, Kudrna D, Currie J, He R, Angelova A, Rajasekar S, Mueller T, Lomeli R, Scara G, Ko A, Delaney K, Wissotski M, Lopez G, Campos D, Braidotti M, Ashley E, Golser W, Kim H, Lee S, Lin J, Dujmic Z, Kim W, Talag J, Zuccolo A, Fan C, Sebastian A, Kramer M, Spiegel L, Nascimento L, Zutavern T, Miller B, Ambroise C, Muller S, Spooner W, Narechania A, Ren L, Wei S, Kumari S, Faga B, Levy MJ, McMahan L, Van Buren P, Vaughn MW, Ying K, Yeh CT, Emrich SJ, Jia Y, Kalyanaraman A, Hsia AP, Barbazuk WB, Baucom RS, Brutnell TP, Carpita NC, Chaparro C, Chia JM, Deragon JM, Estill JC, Fu Y, Jeddeloh JA, Han Y, Lee H, Li P, Lisch DR, Liu S, Liu Z, Nagel DH, McCann MC, SanMiguel P, Myers AM, Nettleton D, Nguyen J, Penning BW, Ponnala L, Schneider KL, Schwartz DC, Sharma A, Soderlund C, Springer NM, Sun Q, Wang H, Waterman M, Westerman R, Wolfgruber TK, Yang L, Yu Y, Zhang L, Zhou S, Zhu Q, Bennetzen JL, Dawe RK, Jiang J, Jiang N, Presting GG, Wessler SR, Aluru S, Martienssen RA, Clifton SW, McCombie WR, Wing RA, Wilson RK
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)

The maize HMGA protein is localized to the nucleolus and can be acetylated in vitro at its globular domain, and phosphorylation by CDK reduces its binding activity to AT-rich DNA.
Zhao J, Paul LK, Grafi G
Biochimica et biophysica acta1789(11-12)751-7(2009 Nov-Dec)

Characterization of the maize endosperm transcriptome and its comparison to the rice genome.
Lai J, Dey N, Kim CS, Bharti AK, Rudd S, Mayer KF, Larkins BA, Becraft P, Messing J
Genome research14(10A)1932-7(2004 Oct)

Maize chromosomal HMGc. Two closely related structure-specific DNA-binding proteins specify a second type of plant high mobility group box protein.
Grasser KD, Grimm R, Ritt C
The Journal of biological chemistry271(51)32900-6(1996 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that ZmHMGA participates in nucleolar function and that its role may be regulated posttranslationally by phosphorylation and acetylation.
Title: The maize HMGA protein is localized to the nucleolus and can be acetylated in vitro at its globular domain, and phosphorylation by CDK reduces its binding activity to AT-rich DNA.

The following hmg3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hmg3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OZa00477	NM_001112013.1 Latest version!	Zea mays high mobility group protein 3 (LOC542455), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OZa22559	XM_008679571.2 Latest version!	Zea mays high mobility group protein 3 (hmg3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OZa22559	XM_008679570.2 Latest version!	Zea mays high mobility group protein 3 (hmg3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hmg3 ( NM_001112013.1 ) cDNA ORF clone, Zea mays -> NP_001105483.1 Zea mays high mobility group protein 3 (LOC542455), mRNA.

Price & Availability↑

CloneID	OZa00477
Clone ID Related Accession (Same CDS sequence)	NM_001112013.1
Accession Version	NM_001112013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-05
Organism	Zea mays
Product	high mobility group protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y08298.1. ##Evidence-Data-START## Transcript exon combination :: Y08298.1, BT063089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780136, SAMN00780138 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGGCA AGGCCAACGC CTCCAAGAAG GATGAGGCCA GGCTCCGGGC CGGCGGCGGC 
GGCGCCGGAA AGCGCAAGAA GGCCGCCGCG AGCGGCAAGC CCAAGCGCCC TCCCTCCGCC 
TTCTTCGTCT TCATGTCTGA ATTCAGGCAG GAGTACCAGG CGCAGCACCC TGGCAACAAG 
AGCGTTGCGG CCGTGAGCAA GGCAGCAGGG GAAAAGTGGC GCTCTATGTC AGAGCAAGAG 
AAGCAACCCT ATGTGGACCA AGCTGGGCAG AAGAAGCAGG ACTATGAGAA GACTAAGGCA 
AATATTGAGA AGAGCACAAG CTCAAAGAAG GCTAAGACTG ATGACGACGA TGGCTCGAAG 
TCCGAGGTCG ATGATGAGGA TGGCGGTAGT GACGAGGATA ACGACGACGA CGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001105483.1
CDS	1..417
Translation	MKGKANASKKDEARLRAGGGGAGKRKKAAASGKPKRPPSAFFVFMSEFRQEYQAQHPGNKSVAAVSKAAGEKWRSMSEQEKQPYVDQAGQKKQDYEKTKANIEKSTSSKKAKTDDDDGSKSEVDDEDGGSDEDNDDDE

Target ORF information:

RefSeq Version	NM_001112013.1
Organism	Zea mays
Definition	Zea mays high mobility group protein 3 (LOC542455), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112013.1

ORF Insert Sequence:

ATGAAGGGCA AGGCCAACGC CTCCAAGAAG GATGAGGCCA GGCTCCGGGC CGGCGGCGGC 
GGCGCCGGAA AGCGCAAGAA GGCCGCCGCG AGCGGCAAGC CCAAGCGCCC TCCCTCCGCC 
TTCTTCGTCT TCATGTCTGA ATTCAGGCAG GAGTACCAGG CGCAGCACCC TGGCAACAAG 
AGCGTTGCGG CCGTGAGCAA GGCAGCAGGG GAAAAGTGGC GCTCTATGTC AGAGCAAGAG 
AAGCAACCCT ATGTGGACCA AGCTGGGCAG AAGAAGCAGG ACTATGAGAA GACTAAGGCA 
AATATTGAGA AGAGCACAAG CTCAAAGAAG GCTAAGACTG ATGACGACGA TGGCTCGAAG 
TCCGAGGTCG ATGATGAGGA TGGCGGTAGT GACGAGGATA ACGACGACGA CGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmg3 ( XM_008679570.2 ) cDNA ORF clone, Zea mays -> XP_008677792.1 Zea mays high mobility group protein 3 (hmg3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OZa22559
Clone ID Related Accession (Same CDS sequence)	XM_008679570.2 , XM_008679571.2
Accession Version	XM_008679570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Zea mays
Product	high mobility group protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024462.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2017 this sequence version replaced XM_008679570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGCA AGGCCAACGC CTCCAAGAAG GATGAGGCCA GGCTCCGGGC CGGCGGCGGC 
GGCGCCGGAA AGCGCAAGAA GGCCGCCGCG AGCGGCAAGC CCAAGCGCCC TCCCTCCGCC 
TTCTTCGTCT TCATGTCTGA ATTCAGGCAG GAGTACCAGG CGCAGCACCC TGGCAACAAG 
AGCGTTGCGG CCGTGAGCAA GGCAGCAGGG GAAAAGTGGC GCTCTATGTC AGAGCAAGAG 
AAGCAACCCT ATGTGGACCA AGCTGGGCAG AAGAAGCAGG ACTATGAGAA GACTAAGGCA 
AATATTGAGA AGGAGAGCAC AAGCTCAAAG AAGGCTAAGA CTGATGACGA CGATGGCTCG 
AAGTCCGAGG TCGATGATGA GGATGGCGGT AGTGACGAGG ATAACGACGA CGACGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008677792.1
CDS	217..636
Translation	MKGKANASKKDEARLRAGGGGAGKRKKAAASGKPKRPPSAFFVFMSEFRQEYQAQHPGNKSVAAVSKAAGEKWRSMSEQEKQPYVDQAGQKKQDYEKTKANIEKESTSSKKAKTDDDDGSKSEVDDEDGGSDEDNDDDE

Target ORF information:

RefSeq Version	XM_008679570.2
Organism	Zea mays
Definition	Zea mays high mobility group protein 3 (hmg3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008679570.2

ORF Insert Sequence:

ATGAAGGGCA AGGCCAACGC CTCCAAGAAG GATGAGGCCA GGCTCCGGGC CGGCGGCGGC 
GGCGCCGGAA AGCGCAAGAA GGCCGCCGCG AGCGGCAAGC CCAAGCGCCC TCCCTCCGCC 
TTCTTCGTCT TCATGTCTGA ATTCAGGCAG GAGTACCAGG CGCAGCACCC TGGCAACAAG 
AGCGTTGCGG CCGTGAGCAA GGCAGCAGGG GAAAAGTGGC GCTCTATGTC AGAGCAAGAG 
AAGCAACCCT ATGTGGACCA AGCTGGGCAG AAGAAGCAGG ACTATGAGAA GACTAAGGCA 
AATATTGAGA AGGAGAGCAC AAGCTCAAAG AAGGCTAAGA CTGATGACGA CGATGGCTCG 
AAGTCCGAGG TCGATGATGA GGATGGCGGT AGTGACGAGG ATAACGACGA CGACGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hmg3 ( XM_008679571.2 ) cDNA ORF clone, Zea mays -> XP_008677793.1 Zea mays high mobility group protein 3 (hmg3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OZa22559
Clone ID Related Accession (Same CDS sequence)	XM_008679570.2 , XM_008679571.2
Accession Version	XM_008679571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Zea mays
Product	high mobility group protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024462.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2017 this sequence version replaced XM_008679571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGCA AGGCCAACGC CTCCAAGAAG GATGAGGCCA GGCTCCGGGC CGGCGGCGGC 
GGCGCCGGAA AGCGCAAGAA GGCCGCCGCG AGCGGCAAGC CCAAGCGCCC TCCCTCCGCC 
TTCTTCGTCT TCATGTCTGA ATTCAGGCAG GAGTACCAGG CGCAGCACCC TGGCAACAAG 
AGCGTTGCGG CCGTGAGCAA GGCAGCAGGG GAAAAGTGGC GCTCTATGTC AGAGCAAGAG 
AAGCAACCCT ATGTGGACCA AGCTGGGCAG AAGAAGCAGG ACTATGAGAA GACTAAGGCA 
AATATTGAGA AGGAGAGCAC AAGCTCAAAG AAGGCTAAGA CTGATGACGA CGATGGCTCG 
AAGTCCGAGG TCGATGATGA GGATGGCGGT AGTGACGAGG ATAACGACGA CGACGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008677793.1
CDS	217..636
Translation	MKGKANASKKDEARLRAGGGGAGKRKKAAASGKPKRPPSAFFVFMSEFRQEYQAQHPGNKSVAAVSKAAGEKWRSMSEQEKQPYVDQAGQKKQDYEKTKANIEKESTSSKKAKTDDDDGSKSEVDDEDGGSDEDNDDDE

Target ORF information:

RefSeq Version	XM_008679571.2
Organism	Zea mays
Definition	Zea mays high mobility group protein 3 (hmg3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008679571.2

ORF Insert Sequence:

ATGAAGGGCA AGGCCAACGC CTCCAAGAAG GATGAGGCCA GGCTCCGGGC CGGCGGCGGC 
GGCGCCGGAA AGCGCAAGAA GGCCGCCGCG AGCGGCAAGC CCAAGCGCCC TCCCTCCGCC 
TTCTTCGTCT TCATGTCTGA ATTCAGGCAG GAGTACCAGG CGCAGCACCC TGGCAACAAG 
AGCGTTGCGG CCGTGAGCAA GGCAGCAGGG GAAAAGTGGC GCTCTATGTC AGAGCAAGAG 
AAGCAACCCT ATGTGGACCA AGCTGGGCAG AAGAAGCAGG ACTATGAGAA GACTAAGGCA 
AATATTGAGA AGGAGAGCAC AAGCTCAAAG AAGGCTAAGA CTGATGACGA CGATGGCTCG 
AAGTCCGAGG TCGATGATGA GGATGGCGGT AGTGACGAGG ATAACGACGA CGACGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The B73 maize genome: complexity, diversity, and dynamics.
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)
Schnable PS,Ware D,Fulton RS,Stein JC,Wei F,Pasternak S,Liang C,Zhang J,Fulton L,Graves TA,Minx P,Reily AD,Courtney L,Kruchowski SS,Tomlinson C,Strong C,Delehaunty K,Fronick C,Courtney B,Rock SM,Belter E,Du F,Kim K,Abbott RM,Cotton M,Levy A,Marchetto P,Ochoa K,Jackson SM,Gillam B,Chen W,Yan L,Higginbotham J,Cardenas M,Waligorski J,Applebaum E,Phelps L,Falcone J,Kanchi K,Thane T,Scimone A,Thane N,Henke J,Wang T,Ruppert J,Shah N,Rotter K,Hodges J,Ingenthron E,Cordes M,Kohlberg S,Sgro J,Delgado B,Mead K,Chinwalla A,Leonard S,Crouse K,Collura K,Kudrna D,Currie J,He R,Angelova A,Rajasekar S,Mueller T,Lomeli R,Scara G,Ko A,Delaney K,Wissotski M,Lopez G,Campos D,Braidotti M,Ashley E,Golser W,Kim H,Lee S,Lin J,Dujmic Z,Kim W,Talag J,Zuccolo A,Fan C,Sebastian A,Kramer M,Spiegel L,Nascimento L,Zutavern T,Miller B,Ambroise C,Muller S,Spooner W,Narechania A,Ren L,Wei S,Kumari S,Faga B,Levy MJ,McMahan L,Van Buren P,Vaughn MW,Ying K,Yeh CT,Emrich SJ,Jia Y,Kalyanaraman A,Hsia AP,Barbazuk WB,Baucom RS,Brutnell TP,Carpita NC,Chaparro C,Chia JM,Deragon JM,Estill JC,Fu Y,Jeddeloh JA,Han Y,Lee H,Li P,Lisch DR,Liu S,Liu Z,Nagel DH,McCann MC,SanMiguel P,Myers AM,Nettleton D,Nguyen J,Penning BW,Ponnala L,Schneider KL,Schwartz DC,Sharma A,Soderlund C,Springer NM,Sun Q,Wang H,Waterman M,Westerman R,Wolfgruber TK,Yang L,Yu Y,Zhang L,Zhou S,Zhu Q,Bennetzen JL,Dawe RK,Jiang J,Jiang N,Presting GG,Wessler SR,Aluru S,Martienssen RA,Clifton SW,McCombie WR,Wing RA,Wilson RK

The maize HMGA protein is localized to the nucleolus and can be acetylated in vitro at its globular domain, and phosphorylation by CDK reduces its binding activity to AT-rich DNA.
Biochimica et biophysica acta1789(11-12)751-7(2009 Nov-Dec)
Zhao J,Paul LK,Grafi G

Characterization of the maize endosperm transcriptome and its comparison to the rice genome.
Genome research14(10A)1932-7(2004 Oct)
Lai J,Dey N,Kim CS,Bharti AK,Rudd S,Mayer KF,Larkins BA,Becraft P,Messing J

Maize chromosomal HMGc. Two closely related structure-specific DNA-binding proteins specify a second type of plant high mobility group box protein.
The Journal of biological chemistry271(51)32900-6(1996 Dec)
Grasser KD,Grimm R,Ritt C

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hmg3 cDNA ORF clone, Zea mays

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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