Oligo automation, in the context of molecular biology and biotechnology, refers to the process of automating the synthesis and handling of short DNA or RNA molecules called oligonucleotides (oligos). Oligos are essential tools in various laboratory applications, including DNA sequencing, PCR (Polymerase Chain Reaction), DNA cloning, and gene expression analysis.
Oligo automation involves the use of specialized instruments and robotic systems to perform tasks related to oligonucleotide synthesis, purification, handling, and quality control. Some common aspects of oligo automation include:
1. Synthesis: Automated synthesizers are used to create oligonucleotides with precise sequences. These machines add one nucleotide at a time, ensuring accuracy and consistency.
2. Purification: After synthesis, automated purification systems are employed to remove impurities and ensure that the oligonucleotides are of high purity.
3. Quality Control: Automation systems can perform quality control checks, such as analyzing the length, sequence, and concentration of oligonucleotides, to verify their integrity.
4. Liquid Handling: Robotic platforms are used for accurate liquid handling and dispensing of oligonucleotide samples, reducing the risk of contamination and human error.
5. Library Preparation: In high-throughput sequencing applications, automation is crucial for preparing libraries of DNA fragments that include adapters and barcodes.
6. Plate Filling: Automation is used to transfer oligonucleotides into microplates for storage and distribution.
Oligo automation offers several advantages, including increased efficiency, reproducibility, and reduced labor costs. It is particularly important in high-throughput research and diagnostic laboratories where a large number of oligos are needed for various applications. Automation ensures that oligonucleotides are produced accurately and consistently, contributing to the success of molecular biology experiments and assays.
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GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
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