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Rabbit monoclonal antibody

Introduction

Rabbit monoclonal antibodies (RabMAbs) are high-affinity, highly specific antibodies derived from rabbits. Compared to traditional murine monoclonal antibodies, rabbit antibodies offer enhanced immune responses, superior specificity for complex epitopes, and improved recognition of post-translational modifications. These properties make them valuable in research, diagnostics, and therapeutic development.

Characteristics of Rabbit Monoclonal Antibodies

  • High Affinity: Rabbit immune systems generate antibodies with affinities typically 10- to 100-fold higher than those from mice. This high affinity enables the sensitive detection of low-abundance targets.
  • Broad Epitope Recognition: Rabbits recognize a wider range of epitopes, including those poorly immunogenic in mice. Enhanced ability to bind small molecules, peptides, and subtle structural differences like post-translational modifications.
  • Improved Specificity: Reduced cross-reactivity compared to mouse antibodies, leading to cleaner results in applications like Western blotting and immunohistochemistry.
  • Stability: Rabbit monoclonal antibodies are structurally stable, maintaining activity under challenging experimental conditions.

Production of Rabbit Monoclonal Antibodies

  • Immunization: Rabbits are immunized with the target antigen, producing a diverse pool of B cells that generate high-affinity antibodies.
  • Isolation of Antibody-Producing B Cells: Rabbit B cells are harvested from peripheral blood, spleen, or bone marrow. B cell populations are enriched based on antigen specificity.
  • Fusion or Recombinant Techniques:
    • Hybridoma Technology: B cells are fused with rabbit myeloma cells to create hybridomas, capable of continuous antibody production.
    • Phage Display or Single B Cell Cloning: Antibody genes are cloned from individual rabbit B cells and expressed in recombinant systems.
  • Expression and Purification: Selected monoclonal antibodies are expressed in host systems (e.g., mammalian cells) and purified using affinity chromatography.
  • Characterization: Binding affinity, epitope specificity, and isotype are evaluated to confirm antibody quality and functionality.

Advantages of Rabbit Monoclonal Antibodies

  • Enhanced Epitope Recognition: Ability to generate antibodies against epitopes that are weakly immunogenic or not recognized by mice, including phosphoepitopes and small molecule modifications.
  • High Sensitivity and Signal Clarity: Superior affinity and specificity reduce background noise in assays, ensuring high signal-to-noise ratios.
  • Robust Performance in Diverse Applications: RabMAbs perform consistently across techniques such as ELISA, flow cytometry, and immunohistochemistry.
  • Ease of Humanization: Rabbit antibodies can be humanized for therapeutic applications to reduce immunogenicity in humans.

Applications of Rabbit Monoclonal Antibodies

  • Research: Clean signals with minimal cross-reactivity make RabMAbs ideal for protein detection in Western blotting, immunohistochemistry, and ChIP (Chromatin Immunoprecipitation).
  • Diagnostics: Used in ELISA and lateral flow assays for detecting disease biomarkers, including cancer antigens, infectious agents, and inflammatory markers.
  • Therapeutics: Rabbit antibodies are being developed into therapeutic agents for cancer, autoimmune diseases, and infectious diseases.
  • Post-Translational Modification Studies: Recognize phosphorylated, acetylated, and methylated epitopes, enabling detailed analyses of cellular signaling pathways.

Challenges in Rabbit Monoclonal Antibody Production

  • Technical Complexity: Hybridoma generation in rabbits is more challenging than in mice, requiring specialized techniques.
  • Cost and Time: Rabbit antibody development is more expensive and time-intensive compared to mouse systems.
  • Reproducibility: Early methods suffered from batch-to-batch variability; recombinant expression has largely addressed this issue.

Advances in Rabbit Monoclonal Antibody Technology

  • Recombinant Antibody Platforms: Single B cell cloning and phage display enable the production of highly specific rabbit antibodies in a reproducible manner.
  • High-Throughput Screening: Advanced technologies allow rapid identification of high-affinity antibodies from diverse rabbit B cell repertoires.
  • Humanization Techniques: Genetic engineering tools improve the development of rabbit-derived therapeutic antibodies with reduced immunogenicity.

GenScript Services for Rabbit Monoclonal Antibodies

  • Custom RabMAb Development: GenScript provides tailored solutions, including antigen design, rabbit immunization, and hybridoma or recombinant antibody generation.
  • High-Affinity Antibody Screening: Advanced screening platforms ensure the selection of antibodies with superior specificity and affinity.

Conclusion

Rabbit monoclonal antibodies represent a significant advancement in antibody technology, offering superior affinity, specificity, and versatility for various applications. Their ability to recognize complex and challenging epitopes makes them invaluable in research, diagnostics, and therapeutic development. Ongoing innovations in production technologies, such as recombinant platforms and high-throughput screening, continue to enhance the accessibility and utility of RabMAbs in modern science and medicine.


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Service and Products

Antibody Production Services

Comprehensive custom antibody production services including monoclonal and polyclonal antibody production services with industry leading turnaround times.

Polyclonal Antibody Services

The fastest custom polyclonal antibody production services in the industry with unmatched guarantees.

Custom Monoclonal Antibody Development Services

Developing custom monoclonal antibodies with standard or fully custom protocols including everything from antigen synthesis to antibody scale-up options.

Phospho-Specific Antibody Services

Guaranteed ELISA titer of ≥ 1:64,000 and < 10% cross reactivity with non-phospho peptide.

Immunoassay Development Services

Our custom immunoassay development service offering comprehensive high-throughput assay development with fast turnaround and high efficiency.

Large-scale Antibody Manufacturing

Our large-scale antibody manufacturing services offer polyclonal and monoclonal antibody production of gram quantities for industrial antibody yields.

COVID-19 Reagent Antibodies

On demand SARS-CoV-2 control mAbs ideal for diagnostics, therapeutic, and vaccine development.

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