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Protein expression systems are essential tools in biotechnology and life sciences, enabling the synthesis of recombinant proteins for use in therapeutics, diagnostics, and research. These systems utilize host organisms such as bacteria, mammalian cells, or insect cells to produce proteins with desired characteristics.
E. coli is a widely used bacterial host for recombinant protein production, offering simplicity and cost-effectiveness.
CHO cells are mammalian systems renowned for their ability to perform human-like PTMs, particularly glycosylation, crucial for therapeutic proteins.
The Baculovirus/Insect Cell System is a versatile eukaryotic expression platform leveraging the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to infect insect cells, such as Spodoptera frugiperda (Sf9 or Sf21) or Trichoplusia ni (Hi-5).
Protein expression systems form the backbone of modern molecular biology and biopharmaceutical production. While bacterial systems like E. coli provide rapid, cost-effective solutions, mammalian systems such as CHO cells are essential for producing complex proteins with human-like modifications. The Baculovirus/Insect Cell Expression System complements bacterial and mammalian platforms by providing a balance between PTM capability and cost-efficiency. Continuous optimization of these systems for efficiency and specificity remains a critical area of innovation in biotechnology and life sciences.
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
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If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]