EMC1 cDNA ORF clone, Canis lupus familiaris(Dog)

  • Gene

  • Clones

  • gRNAs

The following EMC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe03711 XM_005617887.3
Latest version!
Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03712 XM_005617888.3
Latest version!
Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03713 XM_005617889.3
Latest version!
Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03714 XM_005617890.3
Latest version!
Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03715 XM_005617891.3
Latest version!
Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03716 XM_850160.5
Latest version!
Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03718 XM_005617893.3
Latest version!
Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
OCe03712 XM_005617888.2 Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03715 XM_005617891.2 Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03711 XM_005617887.2 Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03713 XM_005617889.2 Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03716 XM_850160.4 Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03718 XM_005617893.2 Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe03714 XM_005617890.2 Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe03711
    Clone ID Related Accession (Same CDS sequence) XM_005617887.2 , XM_005617887.3
    Accession Version XM_005617887.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER membrane protein complex subunit 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGCA
    ATTCAAGACG GCTTACTGGG GATGTTCCTG AAACGCCTCT CGTCCCAGCT CATCCTGCTG
    CAGGCGTGGA CTTCTCACCT CTGGAAGATG TTTTATGATG CTCGGAAGCC CCGCAGTCAG
    ATTAAGAATG AGATCAACAT TGACACCCTA GCCAGAGATG AATTTAATCT CCAGAAGATG
    ATGGTGATGG TGACAGCCTC GGGCAAGCTT TTTGGCATTG AGAGCAGTTC TGGCACCATC
    CTGTGGAAAC AGTATCTCCC CAATGTCAAG CCAGACTCCT CCTTTAAACT GATGGTCCAG
    AGGACTACTG CCCATTTCCC CCACCCCCCG CAGTGCACCC TCCTGGTAAA GGACAAGGAC
    ACGGGCATGA GTTCTCTGTA TGTCTTCAAT CCCATCTTTG GGAAGTGGAG TCAGGTGGCT
    CCCCCAGTGC TGAAGCGTCC CATCTTGCAG TCTTTGCTTC TCCCCATCAT GGATCAAGAC
    TATGCCAAGG TGCTGCTGTT GATCGACGAC GAGTATAAGG TCACAGCGTT CCCAGCCACT
    CGGAATGTCT TGAGACAGCT ACATGAGCTC GCCCCTTCCA TCTTCTTCTA TTTGGTGGAT
    GCAGAACAGG GACGGCTGTG TGGATACCGA CTTCGAAAGG ATCTTACCAC TGAGCTAAGT
    TGGGAGCTGA CTATTCCCCC AGAAGTCCAG CGCATTGTCA AGGTGAAGGG GAAGCGCAGC
    AGTGAGCACG TTCATTCCCA GGGTCGAGTG ATGGGGGACC GCAGCGTGCT CTATAAGAGC
    CTGAACCCCA ACCTGCTGGC CGTCGTGACA GAGAGCACAG ACGTACACCA TGAGCGCACC
    TTCATTGGCA TCTTCCTCGT GGATGGTGTC ACCGGGCGCA TCATCCATTC CTCCGTGCAG
    AGGAAGGCCA AGGGCCCCGT CCACATCGTG CACTCAGAGA ACTGGGTGGT GTACCAGTAC
    TGGAATACCA AGGCCCGGCG CAACGAGTTC ACTGCGCTAG AGCTCTACGA GGGCACCGAG
    CAGTACAATG CCACGGCCTT CAGTTCCTTG GACCGCCCCC AGCTGCCCCA GGTCCTCCAG
    CAGTCCTATA TCTTCCCCTC CTCCATCAGT GCCATGGAAG CCACCATCAC CGAGCGTGGC
    ATCACCAGCC GGCACCTGCT TATTGGGCTA CCTTCTGGAG CAATCCTTTC CCTTCCTAAA
    GCCTTGCTGG ATCCCCGCCG TCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACCTA
    ATCCCATATT CCCCAGACGT GCAGATACAC GCAGAGCGAT TCATTAACTA CAACCAGACG
    GTTTCTCGAA TGCGAGGTAT CTACACAGCT CCCTCAGGCC TGGAGTCCAC TTGTTTGGTT
    GTGGCCTATG GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGATGTC
    TTGAAGGATG ACTATGACTA TGTGCTGATC AGCAGTGTCC TCTTTGGCCT GGTCTTTGCC
    ACCATGATCA CTAAGAGACT AGCACAGGTG AAACTCCTGA ATCGGGCCTG GCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617944.1
    CDS18..3014
    Translation

    Target ORF information:

    RefSeq Version XM_005617887.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617887.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGCA
    ATTCAAGACG GCTTACTGGG GATGTTCCTG AAACGCCTCT CGTCCCAGCT CATCCTGCTG
    CAGGCGTGGA CTTCTCACCT CTGGAAGATG TTTTATGATG CTCGGAAGCC CCGCAGTCAG
    ATTAAGAATG AGATCAACAT TGACACCCTA GCCAGAGATG AATTTAATCT CCAGAAGATG
    ATGGTGATGG TGACAGCCTC GGGCAAGCTT TTTGGCATTG AGAGCAGTTC TGGCACCATC
    CTGTGGAAAC AGTATCTCCC CAATGTCAAG CCAGACTCCT CCTTTAAACT GATGGTCCAG
    AGGACTACTG CCCATTTCCC CCACCCCCCG CAGTGCACCC TCCTGGTAAA GGACAAGGAC
    ACGGGCATGA GTTCTCTGTA TGTCTTCAAT CCCATCTTTG GGAAGTGGAG TCAGGTGGCT
    CCCCCAGTGC TGAAGCGTCC CATCTTGCAG TCTTTGCTTC TCCCCATCAT GGATCAAGAC
    TATGCCAAGG TGCTGCTGTT GATCGACGAC GAGTATAAGG TCACAGCGTT CCCAGCCACT
    CGGAATGTCT TGAGACAGCT ACATGAGCTC GCCCCTTCCA TCTTCTTCTA TTTGGTGGAT
    GCAGAACAGG GACGGCTGTG TGGATACCGA CTTCGAAAGG ATCTTACCAC TGAGCTAAGT
    TGGGAGCTGA CTATTCCCCC AGAAGTCCAG CGCATTGTCA AGGTGAAGGG GAAGCGCAGC
    AGTGAGCACG TTCATTCCCA GGGTCGAGTG ATGGGGGACC GCAGCGTGCT CTATAAGAGC
    CTGAACCCCA ACCTGCTGGC CGTCGTGACA GAGAGCACAG ACGTACACCA TGAGCGCACC
    TTCATTGGCA TCTTCCTCGT GGATGGTGTC ACCGGGCGCA TCATCCATTC CTCCGTGCAG
    AGGAAGGCCA AGGGCCCCGT CCACATCGTG CACTCAGAGA ACTGGGTGGT GTACCAGTAC
    TGGAATACCA AGGCCCGGCG CAACGAGTTC ACTGCGCTAG AGCTCTACGA GGGCACCGAG
    CAGTACAATG CCACGGCCTT CAGTTCCTTG GACCGCCCCC AGCTGCCCCA GGTCCTCCAG
    CAGTCCTATA TCTTCCCCTC CTCCATCAGT GCCATGGAAG CCACCATCAC CGAGCGTGGC
    ATCACCAGCC GGCACCTGCT TATTGGGCTA CCTTCTGGAG CAATCCTTTC CCTTCCTAAA
    GCCTTGCTGG ATCCCCGCCG TCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACCTA
    ATCCCATATT CCCCAGACGT GCAGATACAC GCAGAGCGAT TCATTAACTA CAACCAGACG
    GTTTCTCGAA TGCGAGGTAT CTACACAGCT CCCTCAGGCC TGGAGTCCAC TTGTTTGGTT
    GTGGCCTATG GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGATGTC
    TTGAAGGATG ACTATGACTA TGTGCTGATC AGCAGTGTCC TCTTTGGCCT GGTCTTTGCC
    ACCATGATCA CTAAGAGACT AGCACAGGTG AAACTCCTGA ATCGGGCCTG GCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03711
    Clone ID Related Accession (Same CDS sequence) XM_005617887.2 , XM_005617887.3
    Accession Version XM_005617887.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER membrane protein complex subunit 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGCA
    ATTCAAGACG GCTTACTGGG GATGTTCCTG AAACGCCTCT CGTCCCAGCT CATCCTGCTG
    CAGGCGTGGA CTTCTCACCT CTGGAAGATG TTTTATGATG CTCGGAAGCC CCGCAGTCAG
    ATTAAGAATG AGATCAACAT TGACACCCTA GCCAGAGATG AATTTAATCT CCAGAAGATG
    ATGGTGATGG TGACAGCCTC GGGCAAGCTT TTTGGCATTG AGAGCAGTTC TGGCACCATC
    CTGTGGAAAC AGTATCTCCC CAATGTCAAG CCAGACTCCT CCTTTAAACT GATGGTCCAG
    AGGACTACTG CCCATTTCCC CCACCCCCCG CAGTGCACCC TCCTGGTAAA GGACAAGGAC
    ACGGGCATGA GTTCTCTGTA TGTCTTCAAT CCCATCTTTG GGAAGTGGAG TCAGGTGGCT
    CCCCCAGTGC TGAAGCGTCC CATCTTGCAG TCTTTGCTTC TCCCCATCAT GGATCAAGAC
    TATGCCAAGG TGCTGCTGTT GATCGACGAC GAGTATAAGG TCACAGCGTT CCCAGCCACT
    CGGAATGTCT TGAGACAGCT ACATGAGCTC GCCCCTTCCA TCTTCTTCTA TTTGGTGGAT
    GCAGAACAGG GACGGCTGTG TGGATACCGA CTTCGAAAGG ATCTTACCAC TGAGCTAAGT
    TGGGAGCTGA CTATTCCCCC AGAAGTCCAG CGCATTGTCA AGGTGAAGGG GAAGCGCAGC
    AGTGAGCACG TTCATTCCCA GGGTCGAGTG ATGGGGGACC GCAGCGTGCT CTATAAGAGC
    CTGAACCCCA ACCTGCTGGC CGTCGTGACA GAGAGCACAG ACGTACACCA TGAGCGCACC
    TTCATTGGCA TCTTCCTCGT GGATGGTGTC ACCGGGCGCA TCATCCATTC CTCCGTGCAG
    AGGAAGGCCA AGGGCCCCGT CCACATCGTG CACTCAGAGA ACTGGGTGGT GTACCAGTAC
    TGGAATACCA AGGCCCGGCG CAACGAGTTC ACTGCGCTAG AGCTCTACGA GGGCACCGAG
    CAGTACAATG CCACGGCCTT CAGTTCCTTG GACCGCCCCC AGCTGCCCCA GGTCCTCCAG
    CAGTCCTATA TCTTCCCCTC CTCCATCAGT GCCATGGAAG CCACCATCAC CGAGCGTGGC
    ATCACCAGCC GGCACCTGCT TATTGGGCTA CCTTCTGGAG CAATCCTTTC CCTTCCTAAA
    GCCTTGCTGG ATCCCCGCCG TCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACCTA
    ATCCCATATT CCCCAGACGT GCAGATACAC GCAGAGCGAT TCATTAACTA CAACCAGACG
    GTTTCTCGAA TGCGAGGTAT CTACACAGCT CCCTCAGGCC TGGAGTCCAC TTGTTTGGTT
    GTGGCCTATG GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGATGTC
    TTGAAGGATG ACTATGACTA TGTGCTGATC AGCAGTGTCC TCTTTGGCCT GGTCTTTGCC
    ACCATGATCA CTAAGAGACT AGCACAGGTG AAACTCCTGA ATCGGGCCTG GCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617944.1
    CDS32..3028
    Translation

    Target ORF information:

    RefSeq Version XM_005617887.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617887.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGCA
    ATTCAAGACG GCTTACTGGG GATGTTCCTG AAACGCCTCT CGTCCCAGCT CATCCTGCTG
    CAGGCGTGGA CTTCTCACCT CTGGAAGATG TTTTATGATG CTCGGAAGCC CCGCAGTCAG
    ATTAAGAATG AGATCAACAT TGACACCCTA GCCAGAGATG AATTTAATCT CCAGAAGATG
    ATGGTGATGG TGACAGCCTC GGGCAAGCTT TTTGGCATTG AGAGCAGTTC TGGCACCATC
    CTGTGGAAAC AGTATCTCCC CAATGTCAAG CCAGACTCCT CCTTTAAACT GATGGTCCAG
    AGGACTACTG CCCATTTCCC CCACCCCCCG CAGTGCACCC TCCTGGTAAA GGACAAGGAC
    ACGGGCATGA GTTCTCTGTA TGTCTTCAAT CCCATCTTTG GGAAGTGGAG TCAGGTGGCT
    CCCCCAGTGC TGAAGCGTCC CATCTTGCAG TCTTTGCTTC TCCCCATCAT GGATCAAGAC
    TATGCCAAGG TGCTGCTGTT GATCGACGAC GAGTATAAGG TCACAGCGTT CCCAGCCACT
    CGGAATGTCT TGAGACAGCT ACATGAGCTC GCCCCTTCCA TCTTCTTCTA TTTGGTGGAT
    GCAGAACAGG GACGGCTGTG TGGATACCGA CTTCGAAAGG ATCTTACCAC TGAGCTAAGT
    TGGGAGCTGA CTATTCCCCC AGAAGTCCAG CGCATTGTCA AGGTGAAGGG GAAGCGCAGC
    AGTGAGCACG TTCATTCCCA GGGTCGAGTG ATGGGGGACC GCAGCGTGCT CTATAAGAGC
    CTGAACCCCA ACCTGCTGGC CGTCGTGACA GAGAGCACAG ACGTACACCA TGAGCGCACC
    TTCATTGGCA TCTTCCTCGT GGATGGTGTC ACCGGGCGCA TCATCCATTC CTCCGTGCAG
    AGGAAGGCCA AGGGCCCCGT CCACATCGTG CACTCAGAGA ACTGGGTGGT GTACCAGTAC
    TGGAATACCA AGGCCCGGCG CAACGAGTTC ACTGCGCTAG AGCTCTACGA GGGCACCGAG
    CAGTACAATG CCACGGCCTT CAGTTCCTTG GACCGCCCCC AGCTGCCCCA GGTCCTCCAG
    CAGTCCTATA TCTTCCCCTC CTCCATCAGT GCCATGGAAG CCACCATCAC CGAGCGTGGC
    ATCACCAGCC GGCACCTGCT TATTGGGCTA CCTTCTGGAG CAATCCTTTC CCTTCCTAAA
    GCCTTGCTGG ATCCCCGCCG TCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACCTA
    ATCCCATATT CCCCAGACGT GCAGATACAC GCAGAGCGAT TCATTAACTA CAACCAGACG
    GTTTCTCGAA TGCGAGGTAT CTACACAGCT CCCTCAGGCC TGGAGTCCAC TTGTTTGGTT
    GTGGCCTATG GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGATGTC
    TTGAAGGATG ACTATGACTA TGTGCTGATC AGCAGTGTCC TCTTTGGCCT GGTCTTTGCC
    ACCATGATCA CTAAGAGACT AGCACAGGTG AAACTCCTGA ATCGGGCCTG GCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03712
    Clone ID Related Accession (Same CDS sequence) XM_005617888.3 , XM_005617888.2
    Accession Version XM_005617888.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER membrane protein complex subunit 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617888.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGAAGCCTAG CAGTTCTGAA GATGGGTCAC TGGGGAGCTT TGCGGAAAAG
    CCCAGTCCCC AGGACTCATT GACTTGCTTC AACCAGACCT ACACCATTAA TCTCTACTTA
    GTGGAGACCG GTCGGCGGTT GCTCGACACC TCCATCACCT TCAGTTTGGA ACAGAACGGC
    ACTCGGCCAG AGCGGCTCTA CATCCAGGTG TTCTTGAAGA AGGACGACTC TGTGGGCTAC
    CGGGCCTTGG TACAGACAGA GGATCACCTG CTCCTCTTCC TGCAGCAGCT GGCAGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGCAATT
    CAAGACGGCT TACTGGGGAT GTTCCTGAAA CGCCTCTCGT CCCAGCTCAT CCTGCTGCAG
    GCGTGGACTT CTCACCTCTG GAAGATGTTT TATGATGCTC GGAAGCCCCG CAGTCAGATT
    AAGAATGAGA TCAACATTGA CACCCTAGCC AGAGATGAAT TTAATCTCCA GAAGATGATG
    GTGATGGTGA CAGCCTCGGG CAAGCTTTTT GGCATTGAGA GCAGTTCTGG CACCATCCTG
    TGGAAACAGT ATCTCCCCAA TGTCAAGCCA GACTCCTCCT TTAAACTGAT GGTCCAGAGG
    ACTACTGCCC ATTTCCCCCA CCCCCCGCAG TGCACCCTCC TGGTAAAGGA CAAGGACACG
    GGCATGAGTT CTCTGTATGT CTTCAATCCC ATCTTTGGGA AGTGGAGTCA GGTGGCTCCC
    CCAGTGCTGA AGCGTCCCAT CTTGCAGTCT TTGCTTCTCC CCATCATGGA TCAAGACTAT
    GCCAAGGTGC TGCTGTTGAT CGACGACGAG TATAAGGTCA CAGCGTTCCC AGCCACTCGG
    AATGTCTTGA GACAGCTACA TGAGCTCGCC CCTTCCATCT TCTTCTATTT GGTGGATGCA
    GAACAGGGAC GGCTGTGTGG ATACCGACTT CGAAAGGATC TTACCACTGA GCTAAGTTGG
    GAGCTGACTA TTCCCCCAGA AGTCCAGCGC ATTGTCAAGG TGAAGGGGAA GCGCAGCAGT
    GAGCACGTTC ATTCCCAGGG TCGAGTGATG GGGGACCGCA GCGTGCTCTA TAAGAGCCTG
    AACCCCAACC TGCTGGCCGT CGTGACAGAG AGCACAGACG TACACCATGA GCGCACCTTC
    ATTGGCATCT TCCTCGTGGA TGGTGTCACC GGGCGCATCA TCCATTCCTC CGTGCAGAGG
    AAGGCCAAGG GCCCCGTCCA CATCGTGCAC TCAGAGAACT GGGTGGTGTA CCAGTACTGG
    AATACCAAGG CCCGGCGCAA CGAGTTCACT GCGCTAGAGC TCTACGAGGG CACCGAGCAG
    TACAATGCCA CGGCCTTCAG TTCCTTGGAC CGCCCCCAGC TGCCCCAGGT CCTCCAGCAG
    TCCTATATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCCA CCATCACCGA GCGTGGCATC
    ACCAGCCGGC ACCTGCTTAT TGGGCTACCT TCTGGAGCAA TCCTTTCCCT TCCTAAAGCC
    TTGCTGGATC CCCGCCGTCC CGAGATCCCA ACAGAACAAA GCAGAGAGGA GAACCTAATC
    CCATATTCCC CAGACGTGCA GATACACGCA GAGCGATTCA TTAACTACAA CCAGACGGTT
    TCTCGAATGC GAGGTATCTA CACAGCTCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG
    GCCTATGGTT TGGACATTTA CCAAACTCGA GTCTACCCAT CCAAGCAGTT TGATGTCTTG
    AAGGATGACT ATGACTATGT GCTGATCAGC AGTGTCCTCT TTGGCCTGGT CTTTGCCACC
    ATGATCACTA AGAGACTAGC ACAGGTGAAA CTCCTGAATC GGGCCTGGCG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617945.1
    CDS32..3025
    Translation

    Target ORF information:

    RefSeq Version XM_005617888.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617888.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGAAGCCTAG CAGTTCTGAA GATGGGTCAC TGGGGAGCTT TGCGGAAAAG
    CCCAGTCCCC AGGACTCATT GACTTGCTTC AACCAGACCT ACACCATTAA TCTCTACTTA
    GTGGAGACCG GTCGGCGGTT GCTCGACACC TCCATCACCT TCAGTTTGGA ACAGAACGGC
    ACTCGGCCAG AGCGGCTCTA CATCCAGGTG TTCTTGAAGA AGGACGACTC TGTGGGCTAC
    CGGGCCTTGG TACAGACAGA GGATCACCTG CTCCTCTTCC TGCAGCAGCT GGCAGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGCAATT
    CAAGACGGCT TACTGGGGAT GTTCCTGAAA CGCCTCTCGT CCCAGCTCAT CCTGCTGCAG
    GCGTGGACTT CTCACCTCTG GAAGATGTTT TATGATGCTC GGAAGCCCCG CAGTCAGATT
    AAGAATGAGA TCAACATTGA CACCCTAGCC AGAGATGAAT TTAATCTCCA GAAGATGATG
    GTGATGGTGA CAGCCTCGGG CAAGCTTTTT GGCATTGAGA GCAGTTCTGG CACCATCCTG
    TGGAAACAGT ATCTCCCCAA TGTCAAGCCA GACTCCTCCT TTAAACTGAT GGTCCAGAGG
    ACTACTGCCC ATTTCCCCCA CCCCCCGCAG TGCACCCTCC TGGTAAAGGA CAAGGACACG
    GGCATGAGTT CTCTGTATGT CTTCAATCCC ATCTTTGGGA AGTGGAGTCA GGTGGCTCCC
    CCAGTGCTGA AGCGTCCCAT CTTGCAGTCT TTGCTTCTCC CCATCATGGA TCAAGACTAT
    GCCAAGGTGC TGCTGTTGAT CGACGACGAG TATAAGGTCA CAGCGTTCCC AGCCACTCGG
    AATGTCTTGA GACAGCTACA TGAGCTCGCC CCTTCCATCT TCTTCTATTT GGTGGATGCA
    GAACAGGGAC GGCTGTGTGG ATACCGACTT CGAAAGGATC TTACCACTGA GCTAAGTTGG
    GAGCTGACTA TTCCCCCAGA AGTCCAGCGC ATTGTCAAGG TGAAGGGGAA GCGCAGCAGT
    GAGCACGTTC ATTCCCAGGG TCGAGTGATG GGGGACCGCA GCGTGCTCTA TAAGAGCCTG
    AACCCCAACC TGCTGGCCGT CGTGACAGAG AGCACAGACG TACACCATGA GCGCACCTTC
    ATTGGCATCT TCCTCGTGGA TGGTGTCACC GGGCGCATCA TCCATTCCTC CGTGCAGAGG
    AAGGCCAAGG GCCCCGTCCA CATCGTGCAC TCAGAGAACT GGGTGGTGTA CCAGTACTGG
    AATACCAAGG CCCGGCGCAA CGAGTTCACT GCGCTAGAGC TCTACGAGGG CACCGAGCAG
    TACAATGCCA CGGCCTTCAG TTCCTTGGAC CGCCCCCAGC TGCCCCAGGT CCTCCAGCAG
    TCCTATATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCCA CCATCACCGA GCGTGGCATC
    ACCAGCCGGC ACCTGCTTAT TGGGCTACCT TCTGGAGCAA TCCTTTCCCT TCCTAAAGCC
    TTGCTGGATC CCCGCCGTCC CGAGATCCCA ACAGAACAAA GCAGAGAGGA GAACCTAATC
    CCATATTCCC CAGACGTGCA GATACACGCA GAGCGATTCA TTAACTACAA CCAGACGGTT
    TCTCGAATGC GAGGTATCTA CACAGCTCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG
    GCCTATGGTT TGGACATTTA CCAAACTCGA GTCTACCCAT CCAAGCAGTT TGATGTCTTG
    AAGGATGACT ATGACTATGT GCTGATCAGC AGTGTCCTCT TTGGCCTGGT CTTTGCCACC
    ATGATCACTA AGAGACTAGC ACAGGTGAAA CTCCTGAATC GGGCCTGGCG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03712
    Clone ID Related Accession (Same CDS sequence) XM_005617888.3 , XM_005617888.2
    Accession Version XM_005617888.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER membrane protein complex subunit 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGAAGCCTAG CAGTTCTGAA GATGGGTCAC TGGGGAGCTT TGCGGAAAAG
    CCCAGTCCCC AGGACTCATT GACTTGCTTC AACCAGACCT ACACCATTAA TCTCTACTTA
    GTGGAGACCG GTCGGCGGTT GCTCGACACC TCCATCACCT TCAGTTTGGA ACAGAACGGC
    ACTCGGCCAG AGCGGCTCTA CATCCAGGTG TTCTTGAAGA AGGACGACTC TGTGGGCTAC
    CGGGCCTTGG TACAGACAGA GGATCACCTG CTCCTCTTCC TGCAGCAGCT GGCAGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGCAATT
    CAAGACGGCT TACTGGGGAT GTTCCTGAAA CGCCTCTCGT CCCAGCTCAT CCTGCTGCAG
    GCGTGGACTT CTCACCTCTG GAAGATGTTT TATGATGCTC GGAAGCCCCG CAGTCAGATT
    AAGAATGAGA TCAACATTGA CACCCTAGCC AGAGATGAAT TTAATCTCCA GAAGATGATG
    GTGATGGTGA CAGCCTCGGG CAAGCTTTTT GGCATTGAGA GCAGTTCTGG CACCATCCTG
    TGGAAACAGT ATCTCCCCAA TGTCAAGCCA GACTCCTCCT TTAAACTGAT GGTCCAGAGG
    ACTACTGCCC ATTTCCCCCA CCCCCCGCAG TGCACCCTCC TGGTAAAGGA CAAGGACACG
    GGCATGAGTT CTCTGTATGT CTTCAATCCC ATCTTTGGGA AGTGGAGTCA GGTGGCTCCC
    CCAGTGCTGA AGCGTCCCAT CTTGCAGTCT TTGCTTCTCC CCATCATGGA TCAAGACTAT
    GCCAAGGTGC TGCTGTTGAT CGACGACGAG TATAAGGTCA CAGCGTTCCC AGCCACTCGG
    AATGTCTTGA GACAGCTACA TGAGCTCGCC CCTTCCATCT TCTTCTATTT GGTGGATGCA
    GAACAGGGAC GGCTGTGTGG ATACCGACTT CGAAAGGATC TTACCACTGA GCTAAGTTGG
    GAGCTGACTA TTCCCCCAGA AGTCCAGCGC ATTGTCAAGG TGAAGGGGAA GCGCAGCAGT
    GAGCACGTTC ATTCCCAGGG TCGAGTGATG GGGGACCGCA GCGTGCTCTA TAAGAGCCTG
    AACCCCAACC TGCTGGCCGT CGTGACAGAG AGCACAGACG TACACCATGA GCGCACCTTC
    ATTGGCATCT TCCTCGTGGA TGGTGTCACC GGGCGCATCA TCCATTCCTC CGTGCAGAGG
    AAGGCCAAGG GCCCCGTCCA CATCGTGCAC TCAGAGAACT GGGTGGTGTA CCAGTACTGG
    AATACCAAGG CCCGGCGCAA CGAGTTCACT GCGCTAGAGC TCTACGAGGG CACCGAGCAG
    TACAATGCCA CGGCCTTCAG TTCCTTGGAC CGCCCCCAGC TGCCCCAGGT CCTCCAGCAG
    TCCTATATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCCA CCATCACCGA GCGTGGCATC
    ACCAGCCGGC ACCTGCTTAT TGGGCTACCT TCTGGAGCAA TCCTTTCCCT TCCTAAAGCC
    TTGCTGGATC CCCGCCGTCC CGAGATCCCA ACAGAACAAA GCAGAGAGGA GAACCTAATC
    CCATATTCCC CAGACGTGCA GATACACGCA GAGCGATTCA TTAACTACAA CCAGACGGTT
    TCTCGAATGC GAGGTATCTA CACAGCTCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG
    GCCTATGGTT TGGACATTTA CCAAACTCGA GTCTACCCAT CCAAGCAGTT TGATGTCTTG
    AAGGATGACT ATGACTATGT GCTGATCAGC AGTGTCCTCT TTGGCCTGGT CTTTGCCACC
    ATGATCACTA AGAGACTAGC ACAGGTGAAA CTCCTGAATC GGGCCTGGCG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617945.1
    CDS18..3011
    Translation

    Target ORF information:

    RefSeq Version XM_005617888.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617888.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGAAGCCTAG CAGTTCTGAA GATGGGTCAC TGGGGAGCTT TGCGGAAAAG
    CCCAGTCCCC AGGACTCATT GACTTGCTTC AACCAGACCT ACACCATTAA TCTCTACTTA
    GTGGAGACCG GTCGGCGGTT GCTCGACACC TCCATCACCT TCAGTTTGGA ACAGAACGGC
    ACTCGGCCAG AGCGGCTCTA CATCCAGGTG TTCTTGAAGA AGGACGACTC TGTGGGCTAC
    CGGGCCTTGG TACAGACAGA GGATCACCTG CTCCTCTTCC TGCAGCAGCT GGCAGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGCAATT
    CAAGACGGCT TACTGGGGAT GTTCCTGAAA CGCCTCTCGT CCCAGCTCAT CCTGCTGCAG
    GCGTGGACTT CTCACCTCTG GAAGATGTTT TATGATGCTC GGAAGCCCCG CAGTCAGATT
    AAGAATGAGA TCAACATTGA CACCCTAGCC AGAGATGAAT TTAATCTCCA GAAGATGATG
    GTGATGGTGA CAGCCTCGGG CAAGCTTTTT GGCATTGAGA GCAGTTCTGG CACCATCCTG
    TGGAAACAGT ATCTCCCCAA TGTCAAGCCA GACTCCTCCT TTAAACTGAT GGTCCAGAGG
    ACTACTGCCC ATTTCCCCCA CCCCCCGCAG TGCACCCTCC TGGTAAAGGA CAAGGACACG
    GGCATGAGTT CTCTGTATGT CTTCAATCCC ATCTTTGGGA AGTGGAGTCA GGTGGCTCCC
    CCAGTGCTGA AGCGTCCCAT CTTGCAGTCT TTGCTTCTCC CCATCATGGA TCAAGACTAT
    GCCAAGGTGC TGCTGTTGAT CGACGACGAG TATAAGGTCA CAGCGTTCCC AGCCACTCGG
    AATGTCTTGA GACAGCTACA TGAGCTCGCC CCTTCCATCT TCTTCTATTT GGTGGATGCA
    GAACAGGGAC GGCTGTGTGG ATACCGACTT CGAAAGGATC TTACCACTGA GCTAAGTTGG
    GAGCTGACTA TTCCCCCAGA AGTCCAGCGC ATTGTCAAGG TGAAGGGGAA GCGCAGCAGT
    GAGCACGTTC ATTCCCAGGG TCGAGTGATG GGGGACCGCA GCGTGCTCTA TAAGAGCCTG
    AACCCCAACC TGCTGGCCGT CGTGACAGAG AGCACAGACG TACACCATGA GCGCACCTTC
    ATTGGCATCT TCCTCGTGGA TGGTGTCACC GGGCGCATCA TCCATTCCTC CGTGCAGAGG
    AAGGCCAAGG GCCCCGTCCA CATCGTGCAC TCAGAGAACT GGGTGGTGTA CCAGTACTGG
    AATACCAAGG CCCGGCGCAA CGAGTTCACT GCGCTAGAGC TCTACGAGGG CACCGAGCAG
    TACAATGCCA CGGCCTTCAG TTCCTTGGAC CGCCCCCAGC TGCCCCAGGT CCTCCAGCAG
    TCCTATATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCCA CCATCACCGA GCGTGGCATC
    ACCAGCCGGC ACCTGCTTAT TGGGCTACCT TCTGGAGCAA TCCTTTCCCT TCCTAAAGCC
    TTGCTGGATC CCCGCCGTCC CGAGATCCCA ACAGAACAAA GCAGAGAGGA GAACCTAATC
    CCATATTCCC CAGACGTGCA GATACACGCA GAGCGATTCA TTAACTACAA CCAGACGGTT
    TCTCGAATGC GAGGTATCTA CACAGCTCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG
    GCCTATGGTT TGGACATTTA CCAAACTCGA GTCTACCCAT CCAAGCAGTT TGATGTCTTG
    AAGGATGACT ATGACTATGT GCTGATCAGC AGTGTCCTCT TTGGCCTGGT CTTTGCCACC
    ATGATCACTA AGAGACTAGC ACAGGTGAAA CTCCTGAATC GGGCCTGGCG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03713
    Clone ID Related Accession (Same CDS sequence) XM_005617889.3 , XM_005617889.2
    Accession Version XM_005617889.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER membrane protein complex subunit 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGCAATT
    CAAGACGGCT TACTGGGGAT GTTCCTGAAA CGCCTCTCGT CCCAGCTCAT CCTGCTGCAG
    GCGTGGACTT CTCACCTCTG GAAGATGTTT TATGATGCTC GGAAGCCCCG CAGTCAGATT
    AAGAATGAGA TCAACATTGA CACCCTAGCC AGAGATGAAT TTAATCTCCA GAAGATGATG
    GTGATGGTGA CAGCCTCGGG CAAGCTTTTT GGCATTGAGA GCAGTTCTGG CACCATCCTG
    TGGAAACAGT ATCTCCCCAA TGTCAAGCCA GACTCCTCCT TTAAACTGAT GGTCCAGAGG
    ACTACTGCCC ATTTCCCCCA CCCCCCGCAG TGCACCCTCC TGGTAAAGGA CAAGGACACG
    GGCATGAGTT CTCTGTATGT CTTCAATCCC ATCTTTGGGA AGTGGAGTCA GGTGGCTCCC
    CCAGTGCTGA AGCGTCCCAT CTTGCAGTCT TTGCTTCTCC CCATCATGGA TCAAGACTAT
    GCCAAGGTGC TGCTGTTGAT CGACGACGAG TATAAGGTCA CAGCGTTCCC AGCCACTCGG
    AATGTCTTGA GACAGCTACA TGAGCTCGCC CCTTCCATCT TCTTCTATTT GGTGGATGCA
    GAACAGGGAC GGCTGTGTGG ATACCGACTT CGAAAGGATC TTACCACTGA GCTAAGTTGG
    GAGCTGACTA TTCCCCCAGA AGTCCAGCGC ATTGTCAAGG TGAAGGGGAA GCGCAGCAGT
    GAGCACGTTC ATTCCCAGGG TCGAGTGATG GGGGACCGCA GCGTGCTCTA TAAGAGCCTG
    AACCCCAACC TGCTGGCCGT CGTGACAGAG AGCACAGACG TACACCATGA GCGCACCTTC
    ATTGGCATCT TCCTCGTGGA TGGTGTCACC GGGCGCATCA TCCATTCCTC CGTGCAGAGG
    AAGGCCAAGG GCCCCGTCCA CATCGTGCAC TCAGAGAACT GGGTGGTGTA CCAGTACTGG
    AATACCAAGG CCCGGCGCAA CGAGTTCACT GCGCTAGAGC TCTACGAGGG CACCGAGCAG
    TACAATGCCA CGGCCTTCAG TTCCTTGGAC CGCCCCCAGC TGCCCCAGGT CCTCCAGCAG
    TCCTATATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCCA CCATCACCGA GCGTGGCATC
    ACCAGCCGGC ACCTGCTTAT TGGGCTACCT TCTGGAGCAA TCCTTTCCCT TCCTAAAGCC
    TTGCTGGATC CCCGCCGTCC CGAGATCCCA ACAGAACAAA GCAGAGAGGA GAACCTAATC
    CCATATTCCC CAGACGTGCA GATACACGCA GAGCGATTCA TTAACTACAA CCAGACGGTT
    TCTCGAATGC GAGGTATCTA CACAGCTCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG
    GCCTATGGTT TGGACATTTA CCAAACTCGA GTCTACCCAT CCAAGCAGTT TGATGTCTTG
    AAGGATGACT ATGACTATGT GCTGATCAGC AGTGTCCTCT TTGGCCTGGT CTTTGCCACC
    ATGATCACTA AGAGACTAGC ACAGGTGAAA CTCCTGAATC GGGCCTGGCG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617946.1
    CDS32..3025
    Translation

    Target ORF information:

    RefSeq Version XM_005617889.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617889.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGCAATT
    CAAGACGGCT TACTGGGGAT GTTCCTGAAA CGCCTCTCGT CCCAGCTCAT CCTGCTGCAG
    GCGTGGACTT CTCACCTCTG GAAGATGTTT TATGATGCTC GGAAGCCCCG CAGTCAGATT
    AAGAATGAGA TCAACATTGA CACCCTAGCC AGAGATGAAT TTAATCTCCA GAAGATGATG
    GTGATGGTGA CAGCCTCGGG CAAGCTTTTT GGCATTGAGA GCAGTTCTGG CACCATCCTG
    TGGAAACAGT ATCTCCCCAA TGTCAAGCCA GACTCCTCCT TTAAACTGAT GGTCCAGAGG
    ACTACTGCCC ATTTCCCCCA CCCCCCGCAG TGCACCCTCC TGGTAAAGGA CAAGGACACG
    GGCATGAGTT CTCTGTATGT CTTCAATCCC ATCTTTGGGA AGTGGAGTCA GGTGGCTCCC
    CCAGTGCTGA AGCGTCCCAT CTTGCAGTCT TTGCTTCTCC CCATCATGGA TCAAGACTAT
    GCCAAGGTGC TGCTGTTGAT CGACGACGAG TATAAGGTCA CAGCGTTCCC AGCCACTCGG
    AATGTCTTGA GACAGCTACA TGAGCTCGCC CCTTCCATCT TCTTCTATTT GGTGGATGCA
    GAACAGGGAC GGCTGTGTGG ATACCGACTT CGAAAGGATC TTACCACTGA GCTAAGTTGG
    GAGCTGACTA TTCCCCCAGA AGTCCAGCGC ATTGTCAAGG TGAAGGGGAA GCGCAGCAGT
    GAGCACGTTC ATTCCCAGGG TCGAGTGATG GGGGACCGCA GCGTGCTCTA TAAGAGCCTG
    AACCCCAACC TGCTGGCCGT CGTGACAGAG AGCACAGACG TACACCATGA GCGCACCTTC
    ATTGGCATCT TCCTCGTGGA TGGTGTCACC GGGCGCATCA TCCATTCCTC CGTGCAGAGG
    AAGGCCAAGG GCCCCGTCCA CATCGTGCAC TCAGAGAACT GGGTGGTGTA CCAGTACTGG
    AATACCAAGG CCCGGCGCAA CGAGTTCACT GCGCTAGAGC TCTACGAGGG CACCGAGCAG
    TACAATGCCA CGGCCTTCAG TTCCTTGGAC CGCCCCCAGC TGCCCCAGGT CCTCCAGCAG
    TCCTATATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCCA CCATCACCGA GCGTGGCATC
    ACCAGCCGGC ACCTGCTTAT TGGGCTACCT TCTGGAGCAA TCCTTTCCCT TCCTAAAGCC
    TTGCTGGATC CCCGCCGTCC CGAGATCCCA ACAGAACAAA GCAGAGAGGA GAACCTAATC
    CCATATTCCC CAGACGTGCA GATACACGCA GAGCGATTCA TTAACTACAA CCAGACGGTT
    TCTCGAATGC GAGGTATCTA CACAGCTCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG
    GCCTATGGTT TGGACATTTA CCAAACTCGA GTCTACCCAT CCAAGCAGTT TGATGTCTTG
    AAGGATGACT ATGACTATGT GCTGATCAGC AGTGTCCTCT TTGGCCTGGT CTTTGCCACC
    ATGATCACTA AGAGACTAGC ACAGGTGAAA CTCCTGAATC GGGCCTGGCG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03713
    Clone ID Related Accession (Same CDS sequence) XM_005617889.3 , XM_005617889.2
    Accession Version XM_005617889.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER membrane protein complex subunit 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGCAATT
    CAAGACGGCT TACTGGGGAT GTTCCTGAAA CGCCTCTCGT CCCAGCTCAT CCTGCTGCAG
    GCGTGGACTT CTCACCTCTG GAAGATGTTT TATGATGCTC GGAAGCCCCG CAGTCAGATT
    AAGAATGAGA TCAACATTGA CACCCTAGCC AGAGATGAAT TTAATCTCCA GAAGATGATG
    GTGATGGTGA CAGCCTCGGG CAAGCTTTTT GGCATTGAGA GCAGTTCTGG CACCATCCTG
    TGGAAACAGT ATCTCCCCAA TGTCAAGCCA GACTCCTCCT TTAAACTGAT GGTCCAGAGG
    ACTACTGCCC ATTTCCCCCA CCCCCCGCAG TGCACCCTCC TGGTAAAGGA CAAGGACACG
    GGCATGAGTT CTCTGTATGT CTTCAATCCC ATCTTTGGGA AGTGGAGTCA GGTGGCTCCC
    CCAGTGCTGA AGCGTCCCAT CTTGCAGTCT TTGCTTCTCC CCATCATGGA TCAAGACTAT
    GCCAAGGTGC TGCTGTTGAT CGACGACGAG TATAAGGTCA CAGCGTTCCC AGCCACTCGG
    AATGTCTTGA GACAGCTACA TGAGCTCGCC CCTTCCATCT TCTTCTATTT GGTGGATGCA
    GAACAGGGAC GGCTGTGTGG ATACCGACTT CGAAAGGATC TTACCACTGA GCTAAGTTGG
    GAGCTGACTA TTCCCCCAGA AGTCCAGCGC ATTGTCAAGG TGAAGGGGAA GCGCAGCAGT
    GAGCACGTTC ATTCCCAGGG TCGAGTGATG GGGGACCGCA GCGTGCTCTA TAAGAGCCTG
    AACCCCAACC TGCTGGCCGT CGTGACAGAG AGCACAGACG TACACCATGA GCGCACCTTC
    ATTGGCATCT TCCTCGTGGA TGGTGTCACC GGGCGCATCA TCCATTCCTC CGTGCAGAGG
    AAGGCCAAGG GCCCCGTCCA CATCGTGCAC TCAGAGAACT GGGTGGTGTA CCAGTACTGG
    AATACCAAGG CCCGGCGCAA CGAGTTCACT GCGCTAGAGC TCTACGAGGG CACCGAGCAG
    TACAATGCCA CGGCCTTCAG TTCCTTGGAC CGCCCCCAGC TGCCCCAGGT CCTCCAGCAG
    TCCTATATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCCA CCATCACCGA GCGTGGCATC
    ACCAGCCGGC ACCTGCTTAT TGGGCTACCT TCTGGAGCAA TCCTTTCCCT TCCTAAAGCC
    TTGCTGGATC CCCGCCGTCC CGAGATCCCA ACAGAACAAA GCAGAGAGGA GAACCTAATC
    CCATATTCCC CAGACGTGCA GATACACGCA GAGCGATTCA TTAACTACAA CCAGACGGTT
    TCTCGAATGC GAGGTATCTA CACAGCTCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG
    GCCTATGGTT TGGACATTTA CCAAACTCGA GTCTACCCAT CCAAGCAGTT TGATGTCTTG
    AAGGATGACT ATGACTATGT GCTGATCAGC AGTGTCCTCT TTGGCCTGGT CTTTGCCACC
    ATGATCACTA AGAGACTAGC ACAGGTGAAA CTCCTGAATC GGGCCTGGCG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617946.1
    CDS18..3011
    Translation

    Target ORF information:

    RefSeq Version XM_005617889.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617889.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGCAATT
    CAAGACGGCT TACTGGGGAT GTTCCTGAAA CGCCTCTCGT CCCAGCTCAT CCTGCTGCAG
    GCGTGGACTT CTCACCTCTG GAAGATGTTT TATGATGCTC GGAAGCCCCG CAGTCAGATT
    AAGAATGAGA TCAACATTGA CACCCTAGCC AGAGATGAAT TTAATCTCCA GAAGATGATG
    GTGATGGTGA CAGCCTCGGG CAAGCTTTTT GGCATTGAGA GCAGTTCTGG CACCATCCTG
    TGGAAACAGT ATCTCCCCAA TGTCAAGCCA GACTCCTCCT TTAAACTGAT GGTCCAGAGG
    ACTACTGCCC ATTTCCCCCA CCCCCCGCAG TGCACCCTCC TGGTAAAGGA CAAGGACACG
    GGCATGAGTT CTCTGTATGT CTTCAATCCC ATCTTTGGGA AGTGGAGTCA GGTGGCTCCC
    CCAGTGCTGA AGCGTCCCAT CTTGCAGTCT TTGCTTCTCC CCATCATGGA TCAAGACTAT
    GCCAAGGTGC TGCTGTTGAT CGACGACGAG TATAAGGTCA CAGCGTTCCC AGCCACTCGG
    AATGTCTTGA GACAGCTACA TGAGCTCGCC CCTTCCATCT TCTTCTATTT GGTGGATGCA
    GAACAGGGAC GGCTGTGTGG ATACCGACTT CGAAAGGATC TTACCACTGA GCTAAGTTGG
    GAGCTGACTA TTCCCCCAGA AGTCCAGCGC ATTGTCAAGG TGAAGGGGAA GCGCAGCAGT
    GAGCACGTTC ATTCCCAGGG TCGAGTGATG GGGGACCGCA GCGTGCTCTA TAAGAGCCTG
    AACCCCAACC TGCTGGCCGT CGTGACAGAG AGCACAGACG TACACCATGA GCGCACCTTC
    ATTGGCATCT TCCTCGTGGA TGGTGTCACC GGGCGCATCA TCCATTCCTC CGTGCAGAGG
    AAGGCCAAGG GCCCCGTCCA CATCGTGCAC TCAGAGAACT GGGTGGTGTA CCAGTACTGG
    AATACCAAGG CCCGGCGCAA CGAGTTCACT GCGCTAGAGC TCTACGAGGG CACCGAGCAG
    TACAATGCCA CGGCCTTCAG TTCCTTGGAC CGCCCCCAGC TGCCCCAGGT CCTCCAGCAG
    TCCTATATCT TCCCCTCCTC CATCAGTGCC ATGGAAGCCA CCATCACCGA GCGTGGCATC
    ACCAGCCGGC ACCTGCTTAT TGGGCTACCT TCTGGAGCAA TCCTTTCCCT TCCTAAAGCC
    TTGCTGGATC CCCGCCGTCC CGAGATCCCA ACAGAACAAA GCAGAGAGGA GAACCTAATC
    CCATATTCCC CAGACGTGCA GATACACGCA GAGCGATTCA TTAACTACAA CCAGACGGTT
    TCTCGAATGC GAGGTATCTA CACAGCTCCC TCAGGCCTGG AGTCCACTTG TTTGGTTGTG
    GCCTATGGTT TGGACATTTA CCAAACTCGA GTCTACCCAT CCAAGCAGTT TGATGTCTTG
    AAGGATGACT ATGACTATGT GCTGATCAGC AGTGTCCTCT TTGGCCTGGT CTTTGCCACC
    ATGATCACTA AGAGACTAGC ACAGGTGAAA CTCCTGAATC GGGCCTGGCG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03714
    Clone ID Related Accession (Same CDS sequence) XM_005617890.3 , XM_005617890.2
    Accession Version XM_005617890.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER membrane protein complex subunit 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617890.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGAC
    GGCTTACTGG GGATGTTCCT GAAACGCCTC TCGTCCCAGC TCATCCTGCT GCAGGCGTGG
    ACTTCTCACC TCTGGAAGAT GTTTTATGAT GCTCGGAAGC CCCGCAGTCA GATTAAGAAT
    GAGATCAACA TTGACACCCT AGCCAGAGAT GAATTTAATC TCCAGAAGAT GATGGTGATG
    GTGACAGCCT CGGGCAAGCT TTTTGGCATT GAGAGCAGTT CTGGCACCAT CCTGTGGAAA
    CAGTATCTCC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGGACTACT
    GCCCATTTCC CCCACCCCCC GCAGTGCACC CTCCTGGTAA AGGACAAGGA CACGGGCATG
    AGTTCTCTGT ATGTCTTCAA TCCCATCTTT GGGAAGTGGA GTCAGGTGGC TCCCCCAGTG
    CTGAAGCGTC CCATCTTGCA GTCTTTGCTT CTCCCCATCA TGGATCAAGA CTATGCCAAG
    GTGCTGCTGT TGATCGACGA CGAGTATAAG GTCACAGCGT TCCCAGCCAC TCGGAATGTC
    TTGAGACAGC TACATGAGCT CGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAACAG
    GGACGGCTGT GTGGATACCG ACTTCGAAAG GATCTTACCA CTGAGCTAAG TTGGGAGCTG
    ACTATTCCCC CAGAAGTCCA GCGCATTGTC AAGGTGAAGG GGAAGCGCAG CAGTGAGCAC
    GTTCATTCCC AGGGTCGAGT GATGGGGGAC CGCAGCGTGC TCTATAAGAG CCTGAACCCC
    AACCTGCTGG CCGTCGTGAC AGAGAGCACA GACGTACACC ATGAGCGCAC CTTCATTGGC
    ATCTTCCTCG TGGATGGTGT CACCGGGCGC ATCATCCATT CCTCCGTGCA GAGGAAGGCC
    AAGGGCCCCG TCCACATCGT GCACTCAGAG AACTGGGTGG TGTACCAGTA CTGGAATACC
    AAGGCCCGGC GCAACGAGTT CACTGCGCTA GAGCTCTACG AGGGCACCGA GCAGTACAAT
    GCCACGGCCT TCAGTTCCTT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT
    ATCTTCCCCT CCTCCATCAG TGCCATGGAA GCCACCATCA CCGAGCGTGG CATCACCAGC
    CGGCACCTGC TTATTGGGCT ACCTTCTGGA GCAATCCTTT CCCTTCCTAA AGCCTTGCTG
    GATCCCCGCC GTCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACCT AATCCCATAT
    TCCCCAGACG TGCAGATACA CGCAGAGCGA TTCATTAACT ACAACCAGAC GGTTTCTCGA
    ATGCGAGGTA TCTACACAGC TCCCTCAGGC CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT
    GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGATGT CTTGAAGGAT
    GACTATGACT ATGTGCTGAT CAGCAGTGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC
    ACTAAGAGAC TAGCACAGGT GAAACTCCTG AATCGGGCCT GGCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617947.1
    CDS32..3019
    Translation

    Target ORF information:

    RefSeq Version XM_005617890.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617890.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGAC
    GGCTTACTGG GGATGTTCCT GAAACGCCTC TCGTCCCAGC TCATCCTGCT GCAGGCGTGG
    ACTTCTCACC TCTGGAAGAT GTTTTATGAT GCTCGGAAGC CCCGCAGTCA GATTAAGAAT
    GAGATCAACA TTGACACCCT AGCCAGAGAT GAATTTAATC TCCAGAAGAT GATGGTGATG
    GTGACAGCCT CGGGCAAGCT TTTTGGCATT GAGAGCAGTT CTGGCACCAT CCTGTGGAAA
    CAGTATCTCC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGGACTACT
    GCCCATTTCC CCCACCCCCC GCAGTGCACC CTCCTGGTAA AGGACAAGGA CACGGGCATG
    AGTTCTCTGT ATGTCTTCAA TCCCATCTTT GGGAAGTGGA GTCAGGTGGC TCCCCCAGTG
    CTGAAGCGTC CCATCTTGCA GTCTTTGCTT CTCCCCATCA TGGATCAAGA CTATGCCAAG
    GTGCTGCTGT TGATCGACGA CGAGTATAAG GTCACAGCGT TCCCAGCCAC TCGGAATGTC
    TTGAGACAGC TACATGAGCT CGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAACAG
    GGACGGCTGT GTGGATACCG ACTTCGAAAG GATCTTACCA CTGAGCTAAG TTGGGAGCTG
    ACTATTCCCC CAGAAGTCCA GCGCATTGTC AAGGTGAAGG GGAAGCGCAG CAGTGAGCAC
    GTTCATTCCC AGGGTCGAGT GATGGGGGAC CGCAGCGTGC TCTATAAGAG CCTGAACCCC
    AACCTGCTGG CCGTCGTGAC AGAGAGCACA GACGTACACC ATGAGCGCAC CTTCATTGGC
    ATCTTCCTCG TGGATGGTGT CACCGGGCGC ATCATCCATT CCTCCGTGCA GAGGAAGGCC
    AAGGGCCCCG TCCACATCGT GCACTCAGAG AACTGGGTGG TGTACCAGTA CTGGAATACC
    AAGGCCCGGC GCAACGAGTT CACTGCGCTA GAGCTCTACG AGGGCACCGA GCAGTACAAT
    GCCACGGCCT TCAGTTCCTT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT
    ATCTTCCCCT CCTCCATCAG TGCCATGGAA GCCACCATCA CCGAGCGTGG CATCACCAGC
    CGGCACCTGC TTATTGGGCT ACCTTCTGGA GCAATCCTTT CCCTTCCTAA AGCCTTGCTG
    GATCCCCGCC GTCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACCT AATCCCATAT
    TCCCCAGACG TGCAGATACA CGCAGAGCGA TTCATTAACT ACAACCAGAC GGTTTCTCGA
    ATGCGAGGTA TCTACACAGC TCCCTCAGGC CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT
    GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGATGT CTTGAAGGAT
    GACTATGACT ATGTGCTGAT CAGCAGTGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC
    ACTAAGAGAC TAGCACAGGT GAAACTCCTG AATCGGGCCT GGCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03714
    Clone ID Related Accession (Same CDS sequence) XM_005617890.3 , XM_005617890.2
    Accession Version XM_005617890.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER membrane protein complex subunit 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617890.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGAC
    GGCTTACTGG GGATGTTCCT GAAACGCCTC TCGTCCCAGC TCATCCTGCT GCAGGCGTGG
    ACTTCTCACC TCTGGAAGAT GTTTTATGAT GCTCGGAAGC CCCGCAGTCA GATTAAGAAT
    GAGATCAACA TTGACACCCT AGCCAGAGAT GAATTTAATC TCCAGAAGAT GATGGTGATG
    GTGACAGCCT CGGGCAAGCT TTTTGGCATT GAGAGCAGTT CTGGCACCAT CCTGTGGAAA
    CAGTATCTCC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGGACTACT
    GCCCATTTCC CCCACCCCCC GCAGTGCACC CTCCTGGTAA AGGACAAGGA CACGGGCATG
    AGTTCTCTGT ATGTCTTCAA TCCCATCTTT GGGAAGTGGA GTCAGGTGGC TCCCCCAGTG
    CTGAAGCGTC CCATCTTGCA GTCTTTGCTT CTCCCCATCA TGGATCAAGA CTATGCCAAG
    GTGCTGCTGT TGATCGACGA CGAGTATAAG GTCACAGCGT TCCCAGCCAC TCGGAATGTC
    TTGAGACAGC TACATGAGCT CGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAACAG
    GGACGGCTGT GTGGATACCG ACTTCGAAAG GATCTTACCA CTGAGCTAAG TTGGGAGCTG
    ACTATTCCCC CAGAAGTCCA GCGCATTGTC AAGGTGAAGG GGAAGCGCAG CAGTGAGCAC
    GTTCATTCCC AGGGTCGAGT GATGGGGGAC CGCAGCGTGC TCTATAAGAG CCTGAACCCC
    AACCTGCTGG CCGTCGTGAC AGAGAGCACA GACGTACACC ATGAGCGCAC CTTCATTGGC
    ATCTTCCTCG TGGATGGTGT CACCGGGCGC ATCATCCATT CCTCCGTGCA GAGGAAGGCC
    AAGGGCCCCG TCCACATCGT GCACTCAGAG AACTGGGTGG TGTACCAGTA CTGGAATACC
    AAGGCCCGGC GCAACGAGTT CACTGCGCTA GAGCTCTACG AGGGCACCGA GCAGTACAAT
    GCCACGGCCT TCAGTTCCTT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT
    ATCTTCCCCT CCTCCATCAG TGCCATGGAA GCCACCATCA CCGAGCGTGG CATCACCAGC
    CGGCACCTGC TTATTGGGCT ACCTTCTGGA GCAATCCTTT CCCTTCCTAA AGCCTTGCTG
    GATCCCCGCC GTCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACCT AATCCCATAT
    TCCCCAGACG TGCAGATACA CGCAGAGCGA TTCATTAACT ACAACCAGAC GGTTTCTCGA
    ATGCGAGGTA TCTACACAGC TCCCTCAGGC CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT
    GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGATGT CTTGAAGGAT
    GACTATGACT ATGTGCTGAT CAGCAGTGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC
    ACTAAGAGAC TAGCACAGGT GAAACTCCTG AATCGGGCCT GGCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617947.1
    CDS18..3005
    Translation

    Target ORF information:

    RefSeq Version XM_005617890.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617890.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGAC
    GGCTTACTGG GGATGTTCCT GAAACGCCTC TCGTCCCAGC TCATCCTGCT GCAGGCGTGG
    ACTTCTCACC TCTGGAAGAT GTTTTATGAT GCTCGGAAGC CCCGCAGTCA GATTAAGAAT
    GAGATCAACA TTGACACCCT AGCCAGAGAT GAATTTAATC TCCAGAAGAT GATGGTGATG
    GTGACAGCCT CGGGCAAGCT TTTTGGCATT GAGAGCAGTT CTGGCACCAT CCTGTGGAAA
    CAGTATCTCC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGGACTACT
    GCCCATTTCC CCCACCCCCC GCAGTGCACC CTCCTGGTAA AGGACAAGGA CACGGGCATG
    AGTTCTCTGT ATGTCTTCAA TCCCATCTTT GGGAAGTGGA GTCAGGTGGC TCCCCCAGTG
    CTGAAGCGTC CCATCTTGCA GTCTTTGCTT CTCCCCATCA TGGATCAAGA CTATGCCAAG
    GTGCTGCTGT TGATCGACGA CGAGTATAAG GTCACAGCGT TCCCAGCCAC TCGGAATGTC
    TTGAGACAGC TACATGAGCT CGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAACAG
    GGACGGCTGT GTGGATACCG ACTTCGAAAG GATCTTACCA CTGAGCTAAG TTGGGAGCTG
    ACTATTCCCC CAGAAGTCCA GCGCATTGTC AAGGTGAAGG GGAAGCGCAG CAGTGAGCAC
    GTTCATTCCC AGGGTCGAGT GATGGGGGAC CGCAGCGTGC TCTATAAGAG CCTGAACCCC
    AACCTGCTGG CCGTCGTGAC AGAGAGCACA GACGTACACC ATGAGCGCAC CTTCATTGGC
    ATCTTCCTCG TGGATGGTGT CACCGGGCGC ATCATCCATT CCTCCGTGCA GAGGAAGGCC
    AAGGGCCCCG TCCACATCGT GCACTCAGAG AACTGGGTGG TGTACCAGTA CTGGAATACC
    AAGGCCCGGC GCAACGAGTT CACTGCGCTA GAGCTCTACG AGGGCACCGA GCAGTACAAT
    GCCACGGCCT TCAGTTCCTT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT
    ATCTTCCCCT CCTCCATCAG TGCCATGGAA GCCACCATCA CCGAGCGTGG CATCACCAGC
    CGGCACCTGC TTATTGGGCT ACCTTCTGGA GCAATCCTTT CCCTTCCTAA AGCCTTGCTG
    GATCCCCGCC GTCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACCT AATCCCATAT
    TCCCCAGACG TGCAGATACA CGCAGAGCGA TTCATTAACT ACAACCAGAC GGTTTCTCGA
    ATGCGAGGTA TCTACACAGC TCCCTCAGGC CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT
    GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGATGT CTTGAAGGAT
    GACTATGACT ATGTGCTGAT CAGCAGTGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC
    ACTAAGAGAC TAGCACAGGT GAAACTCCTG AATCGGGCCT GGCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03715
    Clone ID Related Accession (Same CDS sequence) XM_005617891.2 , XM_005617891.3
    Accession Version XM_005617891.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER membrane protein complex subunit 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGAAGCCTAG CAGTTCTGAA GATGGGTCAC TGGGGAGCTT TGCGGAAAAG
    CCCAGTCCCC AGGACTCATT GACTTGCTTC AACCAGACCT ACACCATTAA TCTCTACTTA
    GTGGAGACCG GTCGGCGGTT GCTCGACACC TCCATCACCT TCAGTTTGGA ACAGAACGGC
    ACTCGGCCAG AGCGGCTCTA CATCCAGGTG TTCTTGAAGA AGGACGACTC TGTGGGCTAC
    CGGGCCTTGG TACAGACAGA GGATCACCTG CTCCTCTTCC TGCAGCAGCT GGCAGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGACGGC
    TTACTGGGGA TGTTCCTGAA ACGCCTCTCG TCCCAGCTCA TCCTGCTGCA GGCGTGGACT
    TCTCACCTCT GGAAGATGTT TTATGATGCT CGGAAGCCCC GCAGTCAGAT TAAGAATGAG
    ATCAACATTG ACACCCTAGC CAGAGATGAA TTTAATCTCC AGAAGATGAT GGTGATGGTG
    ACAGCCTCGG GCAAGCTTTT TGGCATTGAG AGCAGTTCTG GCACCATCCT GTGGAAACAG
    TATCTCCCCA ATGTCAAGCC AGACTCCTCC TTTAAACTGA TGGTCCAGAG GACTACTGCC
    CATTTCCCCC ACCCCCCGCA GTGCACCCTC CTGGTAAAGG ACAAGGACAC GGGCATGAGT
    TCTCTGTATG TCTTCAATCC CATCTTTGGG AAGTGGAGTC AGGTGGCTCC CCCAGTGCTG
    AAGCGTCCCA TCTTGCAGTC TTTGCTTCTC CCCATCATGG ATCAAGACTA TGCCAAGGTG
    CTGCTGTTGA TCGACGACGA GTATAAGGTC ACAGCGTTCC CAGCCACTCG GAATGTCTTG
    AGACAGCTAC ATGAGCTCGC CCCTTCCATC TTCTTCTATT TGGTGGATGC AGAACAGGGA
    CGGCTGTGTG GATACCGACT TCGAAAGGAT CTTACCACTG AGCTAAGTTG GGAGCTGACT
    ATTCCCCCAG AAGTCCAGCG CATTGTCAAG GTGAAGGGGA AGCGCAGCAG TGAGCACGTT
    CATTCCCAGG GTCGAGTGAT GGGGGACCGC AGCGTGCTCT ATAAGAGCCT GAACCCCAAC
    CTGCTGGCCG TCGTGACAGA GAGCACAGAC GTACACCATG AGCGCACCTT CATTGGCATC
    TTCCTCGTGG ATGGTGTCAC CGGGCGCATC ATCCATTCCT CCGTGCAGAG GAAGGCCAAG
    GGCCCCGTCC ACATCGTGCA CTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATACCAAG
    GCCCGGCGCA ACGAGTTCAC TGCGCTAGAG CTCTACGAGG GCACCGAGCA GTACAATGCC
    ACGGCCTTCA GTTCCTTGGA CCGCCCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTATATC
    TTCCCCTCCT CCATCAGTGC CATGGAAGCC ACCATCACCG AGCGTGGCAT CACCAGCCGG
    CACCTGCTTA TTGGGCTACC TTCTGGAGCA ATCCTTTCCC TTCCTAAAGC CTTGCTGGAT
    CCCCGCCGTC CCGAGATCCC AACAGAACAA AGCAGAGAGG AGAACCTAAT CCCATATTCC
    CCAGACGTGC AGATACACGC AGAGCGATTC ATTAACTACA ACCAGACGGT TTCTCGAATG
    CGAGGTATCT ACACAGCTCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTATGGT
    TTGGACATTT ACCAAACTCG AGTCTACCCA TCCAAGCAGT TTGATGTCTT GAAGGATGAC
    TATGACTATG TGCTGATCAG CAGTGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACT
    AAGAGACTAG CACAGGTGAA ACTCCTGAAT CGGGCCTGGC GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617948.1
    CDS18..3002
    Translation

    Target ORF information:

    RefSeq Version XM_005617891.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617891.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGAAGCCTAG CAGTTCTGAA GATGGGTCAC TGGGGAGCTT TGCGGAAAAG
    CCCAGTCCCC AGGACTCATT GACTTGCTTC AACCAGACCT ACACCATTAA TCTCTACTTA
    GTGGAGACCG GTCGGCGGTT GCTCGACACC TCCATCACCT TCAGTTTGGA ACAGAACGGC
    ACTCGGCCAG AGCGGCTCTA CATCCAGGTG TTCTTGAAGA AGGACGACTC TGTGGGCTAC
    CGGGCCTTGG TACAGACAGA GGATCACCTG CTCCTCTTCC TGCAGCAGCT GGCAGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGACGGC
    TTACTGGGGA TGTTCCTGAA ACGCCTCTCG TCCCAGCTCA TCCTGCTGCA GGCGTGGACT
    TCTCACCTCT GGAAGATGTT TTATGATGCT CGGAAGCCCC GCAGTCAGAT TAAGAATGAG
    ATCAACATTG ACACCCTAGC CAGAGATGAA TTTAATCTCC AGAAGATGAT GGTGATGGTG
    ACAGCCTCGG GCAAGCTTTT TGGCATTGAG AGCAGTTCTG GCACCATCCT GTGGAAACAG
    TATCTCCCCA ATGTCAAGCC AGACTCCTCC TTTAAACTGA TGGTCCAGAG GACTACTGCC
    CATTTCCCCC ACCCCCCGCA GTGCACCCTC CTGGTAAAGG ACAAGGACAC GGGCATGAGT
    TCTCTGTATG TCTTCAATCC CATCTTTGGG AAGTGGAGTC AGGTGGCTCC CCCAGTGCTG
    AAGCGTCCCA TCTTGCAGTC TTTGCTTCTC CCCATCATGG ATCAAGACTA TGCCAAGGTG
    CTGCTGTTGA TCGACGACGA GTATAAGGTC ACAGCGTTCC CAGCCACTCG GAATGTCTTG
    AGACAGCTAC ATGAGCTCGC CCCTTCCATC TTCTTCTATT TGGTGGATGC AGAACAGGGA
    CGGCTGTGTG GATACCGACT TCGAAAGGAT CTTACCACTG AGCTAAGTTG GGAGCTGACT
    ATTCCCCCAG AAGTCCAGCG CATTGTCAAG GTGAAGGGGA AGCGCAGCAG TGAGCACGTT
    CATTCCCAGG GTCGAGTGAT GGGGGACCGC AGCGTGCTCT ATAAGAGCCT GAACCCCAAC
    CTGCTGGCCG TCGTGACAGA GAGCACAGAC GTACACCATG AGCGCACCTT CATTGGCATC
    TTCCTCGTGG ATGGTGTCAC CGGGCGCATC ATCCATTCCT CCGTGCAGAG GAAGGCCAAG
    GGCCCCGTCC ACATCGTGCA CTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATACCAAG
    GCCCGGCGCA ACGAGTTCAC TGCGCTAGAG CTCTACGAGG GCACCGAGCA GTACAATGCC
    ACGGCCTTCA GTTCCTTGGA CCGCCCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTATATC
    TTCCCCTCCT CCATCAGTGC CATGGAAGCC ACCATCACCG AGCGTGGCAT CACCAGCCGG
    CACCTGCTTA TTGGGCTACC TTCTGGAGCA ATCCTTTCCC TTCCTAAAGC CTTGCTGGAT
    CCCCGCCGTC CCGAGATCCC AACAGAACAA AGCAGAGAGG AGAACCTAAT CCCATATTCC
    CCAGACGTGC AGATACACGC AGAGCGATTC ATTAACTACA ACCAGACGGT TTCTCGAATG
    CGAGGTATCT ACACAGCTCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTATGGT
    TTGGACATTT ACCAAACTCG AGTCTACCCA TCCAAGCAGT TTGATGTCTT GAAGGATGAC
    TATGACTATG TGCTGATCAG CAGTGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACT
    AAGAGACTAG CACAGGTGAA ACTCCTGAAT CGGGCCTGGC GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03715
    Clone ID Related Accession (Same CDS sequence) XM_005617891.2 , XM_005617891.3
    Accession Version XM_005617891.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER membrane protein complex subunit 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGAAGCCTAG CAGTTCTGAA GATGGGTCAC TGGGGAGCTT TGCGGAAAAG
    CCCAGTCCCC AGGACTCATT GACTTGCTTC AACCAGACCT ACACCATTAA TCTCTACTTA
    GTGGAGACCG GTCGGCGGTT GCTCGACACC TCCATCACCT TCAGTTTGGA ACAGAACGGC
    ACTCGGCCAG AGCGGCTCTA CATCCAGGTG TTCTTGAAGA AGGACGACTC TGTGGGCTAC
    CGGGCCTTGG TACAGACAGA GGATCACCTG CTCCTCTTCC TGCAGCAGCT GGCAGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGACGGC
    TTACTGGGGA TGTTCCTGAA ACGCCTCTCG TCCCAGCTCA TCCTGCTGCA GGCGTGGACT
    TCTCACCTCT GGAAGATGTT TTATGATGCT CGGAAGCCCC GCAGTCAGAT TAAGAATGAG
    ATCAACATTG ACACCCTAGC CAGAGATGAA TTTAATCTCC AGAAGATGAT GGTGATGGTG
    ACAGCCTCGG GCAAGCTTTT TGGCATTGAG AGCAGTTCTG GCACCATCCT GTGGAAACAG
    TATCTCCCCA ATGTCAAGCC AGACTCCTCC TTTAAACTGA TGGTCCAGAG GACTACTGCC
    CATTTCCCCC ACCCCCCGCA GTGCACCCTC CTGGTAAAGG ACAAGGACAC GGGCATGAGT
    TCTCTGTATG TCTTCAATCC CATCTTTGGG AAGTGGAGTC AGGTGGCTCC CCCAGTGCTG
    AAGCGTCCCA TCTTGCAGTC TTTGCTTCTC CCCATCATGG ATCAAGACTA TGCCAAGGTG
    CTGCTGTTGA TCGACGACGA GTATAAGGTC ACAGCGTTCC CAGCCACTCG GAATGTCTTG
    AGACAGCTAC ATGAGCTCGC CCCTTCCATC TTCTTCTATT TGGTGGATGC AGAACAGGGA
    CGGCTGTGTG GATACCGACT TCGAAAGGAT CTTACCACTG AGCTAAGTTG GGAGCTGACT
    ATTCCCCCAG AAGTCCAGCG CATTGTCAAG GTGAAGGGGA AGCGCAGCAG TGAGCACGTT
    CATTCCCAGG GTCGAGTGAT GGGGGACCGC AGCGTGCTCT ATAAGAGCCT GAACCCCAAC
    CTGCTGGCCG TCGTGACAGA GAGCACAGAC GTACACCATG AGCGCACCTT CATTGGCATC
    TTCCTCGTGG ATGGTGTCAC CGGGCGCATC ATCCATTCCT CCGTGCAGAG GAAGGCCAAG
    GGCCCCGTCC ACATCGTGCA CTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATACCAAG
    GCCCGGCGCA ACGAGTTCAC TGCGCTAGAG CTCTACGAGG GCACCGAGCA GTACAATGCC
    ACGGCCTTCA GTTCCTTGGA CCGCCCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTATATC
    TTCCCCTCCT CCATCAGTGC CATGGAAGCC ACCATCACCG AGCGTGGCAT CACCAGCCGG
    CACCTGCTTA TTGGGCTACC TTCTGGAGCA ATCCTTTCCC TTCCTAAAGC CTTGCTGGAT
    CCCCGCCGTC CCGAGATCCC AACAGAACAA AGCAGAGAGG AGAACCTAAT CCCATATTCC
    CCAGACGTGC AGATACACGC AGAGCGATTC ATTAACTACA ACCAGACGGT TTCTCGAATG
    CGAGGTATCT ACACAGCTCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTATGGT
    TTGGACATTT ACCAAACTCG AGTCTACCCA TCCAAGCAGT TTGATGTCTT GAAGGATGAC
    TATGACTATG TGCTGATCAG CAGTGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACT
    AAGAGACTAG CACAGGTGAA ACTCCTGAAT CGGGCCTGGC GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617948.1
    CDS32..3016
    Translation

    Target ORF information:

    RefSeq Version XM_005617891.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617891.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGAAGCCTAG CAGTTCTGAA GATGGGTCAC TGGGGAGCTT TGCGGAAAAG
    CCCAGTCCCC AGGACTCATT GACTTGCTTC AACCAGACCT ACACCATTAA TCTCTACTTA
    GTGGAGACCG GTCGGCGGTT GCTCGACACC TCCATCACCT TCAGTTTGGA ACAGAACGGC
    ACTCGGCCAG AGCGGCTCTA CATCCAGGTG TTCTTGAAGA AGGACGACTC TGTGGGCTAC
    CGGGCCTTGG TACAGACAGA GGATCACCTG CTCCTCTTCC TGCAGCAGCT GGCAGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGACGGC
    TTACTGGGGA TGTTCCTGAA ACGCCTCTCG TCCCAGCTCA TCCTGCTGCA GGCGTGGACT
    TCTCACCTCT GGAAGATGTT TTATGATGCT CGGAAGCCCC GCAGTCAGAT TAAGAATGAG
    ATCAACATTG ACACCCTAGC CAGAGATGAA TTTAATCTCC AGAAGATGAT GGTGATGGTG
    ACAGCCTCGG GCAAGCTTTT TGGCATTGAG AGCAGTTCTG GCACCATCCT GTGGAAACAG
    TATCTCCCCA ATGTCAAGCC AGACTCCTCC TTTAAACTGA TGGTCCAGAG GACTACTGCC
    CATTTCCCCC ACCCCCCGCA GTGCACCCTC CTGGTAAAGG ACAAGGACAC GGGCATGAGT
    TCTCTGTATG TCTTCAATCC CATCTTTGGG AAGTGGAGTC AGGTGGCTCC CCCAGTGCTG
    AAGCGTCCCA TCTTGCAGTC TTTGCTTCTC CCCATCATGG ATCAAGACTA TGCCAAGGTG
    CTGCTGTTGA TCGACGACGA GTATAAGGTC ACAGCGTTCC CAGCCACTCG GAATGTCTTG
    AGACAGCTAC ATGAGCTCGC CCCTTCCATC TTCTTCTATT TGGTGGATGC AGAACAGGGA
    CGGCTGTGTG GATACCGACT TCGAAAGGAT CTTACCACTG AGCTAAGTTG GGAGCTGACT
    ATTCCCCCAG AAGTCCAGCG CATTGTCAAG GTGAAGGGGA AGCGCAGCAG TGAGCACGTT
    CATTCCCAGG GTCGAGTGAT GGGGGACCGC AGCGTGCTCT ATAAGAGCCT GAACCCCAAC
    CTGCTGGCCG TCGTGACAGA GAGCACAGAC GTACACCATG AGCGCACCTT CATTGGCATC
    TTCCTCGTGG ATGGTGTCAC CGGGCGCATC ATCCATTCCT CCGTGCAGAG GAAGGCCAAG
    GGCCCCGTCC ACATCGTGCA CTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATACCAAG
    GCCCGGCGCA ACGAGTTCAC TGCGCTAGAG CTCTACGAGG GCACCGAGCA GTACAATGCC
    ACGGCCTTCA GTTCCTTGGA CCGCCCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTATATC
    TTCCCCTCCT CCATCAGTGC CATGGAAGCC ACCATCACCG AGCGTGGCAT CACCAGCCGG
    CACCTGCTTA TTGGGCTACC TTCTGGAGCA ATCCTTTCCC TTCCTAAAGC CTTGCTGGAT
    CCCCGCCGTC CCGAGATCCC AACAGAACAA AGCAGAGAGG AGAACCTAAT CCCATATTCC
    CCAGACGTGC AGATACACGC AGAGCGATTC ATTAACTACA ACCAGACGGT TTCTCGAATG
    CGAGGTATCT ACACAGCTCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTATGGT
    TTGGACATTT ACCAAACTCG AGTCTACCCA TCCAAGCAGT TTGATGTCTT GAAGGATGAC
    TATGACTATG TGCTGATCAG CAGTGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACT
    AAGAGACTAG CACAGGTGAA ACTCCTGAAT CGGGCCTGGC GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03716
    Clone ID Related Accession (Same CDS sequence) XM_850160.5 , XM_850160.4
    Accession Version XM_850160.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER membrane protein complex subunit 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_850160.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGACGGC
    TTACTGGGGA TGTTCCTGAA ACGCCTCTCG TCCCAGCTCA TCCTGCTGCA GGCGTGGACT
    TCTCACCTCT GGAAGATGTT TTATGATGCT CGGAAGCCCC GCAGTCAGAT TAAGAATGAG
    ATCAACATTG ACACCCTAGC CAGAGATGAA TTTAATCTCC AGAAGATGAT GGTGATGGTG
    ACAGCCTCGG GCAAGCTTTT TGGCATTGAG AGCAGTTCTG GCACCATCCT GTGGAAACAG
    TATCTCCCCA ATGTCAAGCC AGACTCCTCC TTTAAACTGA TGGTCCAGAG GACTACTGCC
    CATTTCCCCC ACCCCCCGCA GTGCACCCTC CTGGTAAAGG ACAAGGACAC GGGCATGAGT
    TCTCTGTATG TCTTCAATCC CATCTTTGGG AAGTGGAGTC AGGTGGCTCC CCCAGTGCTG
    AAGCGTCCCA TCTTGCAGTC TTTGCTTCTC CCCATCATGG ATCAAGACTA TGCCAAGGTG
    CTGCTGTTGA TCGACGACGA GTATAAGGTC ACAGCGTTCC CAGCCACTCG GAATGTCTTG
    AGACAGCTAC ATGAGCTCGC CCCTTCCATC TTCTTCTATT TGGTGGATGC AGAACAGGGA
    CGGCTGTGTG GATACCGACT TCGAAAGGAT CTTACCACTG AGCTAAGTTG GGAGCTGACT
    ATTCCCCCAG AAGTCCAGCG CATTGTCAAG GTGAAGGGGA AGCGCAGCAG TGAGCACGTT
    CATTCCCAGG GTCGAGTGAT GGGGGACCGC AGCGTGCTCT ATAAGAGCCT GAACCCCAAC
    CTGCTGGCCG TCGTGACAGA GAGCACAGAC GTACACCATG AGCGCACCTT CATTGGCATC
    TTCCTCGTGG ATGGTGTCAC CGGGCGCATC ATCCATTCCT CCGTGCAGAG GAAGGCCAAG
    GGCCCCGTCC ACATCGTGCA CTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATACCAAG
    GCCCGGCGCA ACGAGTTCAC TGCGCTAGAG CTCTACGAGG GCACCGAGCA GTACAATGCC
    ACGGCCTTCA GTTCCTTGGA CCGCCCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTATATC
    TTCCCCTCCT CCATCAGTGC CATGGAAGCC ACCATCACCG AGCGTGGCAT CACCAGCCGG
    CACCTGCTTA TTGGGCTACC TTCTGGAGCA ATCCTTTCCC TTCCTAAAGC CTTGCTGGAT
    CCCCGCCGTC CCGAGATCCC AACAGAACAA AGCAGAGAGG AGAACCTAAT CCCATATTCC
    CCAGACGTGC AGATACACGC AGAGCGATTC ATTAACTACA ACCAGACGGT TTCTCGAATG
    CGAGGTATCT ACACAGCTCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTATGGT
    TTGGACATTT ACCAAACTCG AGTCTACCCA TCCAAGCAGT TTGATGTCTT GAAGGATGAC
    TATGACTATG TGCTGATCAG CAGTGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACT
    AAGAGACTAG CACAGGTGAA ACTCCTGAAT CGGGCCTGGC GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_855253.2
    CDS32..3016
    Translation

    Target ORF information:

    RefSeq Version XM_850160.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_850160.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGACGGC
    TTACTGGGGA TGTTCCTGAA ACGCCTCTCG TCCCAGCTCA TCCTGCTGCA GGCGTGGACT
    TCTCACCTCT GGAAGATGTT TTATGATGCT CGGAAGCCCC GCAGTCAGAT TAAGAATGAG
    ATCAACATTG ACACCCTAGC CAGAGATGAA TTTAATCTCC AGAAGATGAT GGTGATGGTG
    ACAGCCTCGG GCAAGCTTTT TGGCATTGAG AGCAGTTCTG GCACCATCCT GTGGAAACAG
    TATCTCCCCA ATGTCAAGCC AGACTCCTCC TTTAAACTGA TGGTCCAGAG GACTACTGCC
    CATTTCCCCC ACCCCCCGCA GTGCACCCTC CTGGTAAAGG ACAAGGACAC GGGCATGAGT
    TCTCTGTATG TCTTCAATCC CATCTTTGGG AAGTGGAGTC AGGTGGCTCC CCCAGTGCTG
    AAGCGTCCCA TCTTGCAGTC TTTGCTTCTC CCCATCATGG ATCAAGACTA TGCCAAGGTG
    CTGCTGTTGA TCGACGACGA GTATAAGGTC ACAGCGTTCC CAGCCACTCG GAATGTCTTG
    AGACAGCTAC ATGAGCTCGC CCCTTCCATC TTCTTCTATT TGGTGGATGC AGAACAGGGA
    CGGCTGTGTG GATACCGACT TCGAAAGGAT CTTACCACTG AGCTAAGTTG GGAGCTGACT
    ATTCCCCCAG AAGTCCAGCG CATTGTCAAG GTGAAGGGGA AGCGCAGCAG TGAGCACGTT
    CATTCCCAGG GTCGAGTGAT GGGGGACCGC AGCGTGCTCT ATAAGAGCCT GAACCCCAAC
    CTGCTGGCCG TCGTGACAGA GAGCACAGAC GTACACCATG AGCGCACCTT CATTGGCATC
    TTCCTCGTGG ATGGTGTCAC CGGGCGCATC ATCCATTCCT CCGTGCAGAG GAAGGCCAAG
    GGCCCCGTCC ACATCGTGCA CTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATACCAAG
    GCCCGGCGCA ACGAGTTCAC TGCGCTAGAG CTCTACGAGG GCACCGAGCA GTACAATGCC
    ACGGCCTTCA GTTCCTTGGA CCGCCCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTATATC
    TTCCCCTCCT CCATCAGTGC CATGGAAGCC ACCATCACCG AGCGTGGCAT CACCAGCCGG
    CACCTGCTTA TTGGGCTACC TTCTGGAGCA ATCCTTTCCC TTCCTAAAGC CTTGCTGGAT
    CCCCGCCGTC CCGAGATCCC AACAGAACAA AGCAGAGAGG AGAACCTAAT CCCATATTCC
    CCAGACGTGC AGATACACGC AGAGCGATTC ATTAACTACA ACCAGACGGT TTCTCGAATG
    CGAGGTATCT ACACAGCTCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTATGGT
    TTGGACATTT ACCAAACTCG AGTCTACCCA TCCAAGCAGT TTGATGTCTT GAAGGATGAC
    TATGACTATG TGCTGATCAG CAGTGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACT
    AAGAGACTAG CACAGGTGAA ACTCCTGAAT CGGGCCTGGC GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03716
    Clone ID Related Accession (Same CDS sequence) XM_850160.5 , XM_850160.4
    Accession Version XM_850160.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2985bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER membrane protein complex subunit 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_850160.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGACGGC
    TTACTGGGGA TGTTCCTGAA ACGCCTCTCG TCCCAGCTCA TCCTGCTGCA GGCGTGGACT
    TCTCACCTCT GGAAGATGTT TTATGATGCT CGGAAGCCCC GCAGTCAGAT TAAGAATGAG
    ATCAACATTG ACACCCTAGC CAGAGATGAA TTTAATCTCC AGAAGATGAT GGTGATGGTG
    ACAGCCTCGG GCAAGCTTTT TGGCATTGAG AGCAGTTCTG GCACCATCCT GTGGAAACAG
    TATCTCCCCA ATGTCAAGCC AGACTCCTCC TTTAAACTGA TGGTCCAGAG GACTACTGCC
    CATTTCCCCC ACCCCCCGCA GTGCACCCTC CTGGTAAAGG ACAAGGACAC GGGCATGAGT
    TCTCTGTATG TCTTCAATCC CATCTTTGGG AAGTGGAGTC AGGTGGCTCC CCCAGTGCTG
    AAGCGTCCCA TCTTGCAGTC TTTGCTTCTC CCCATCATGG ATCAAGACTA TGCCAAGGTG
    CTGCTGTTGA TCGACGACGA GTATAAGGTC ACAGCGTTCC CAGCCACTCG GAATGTCTTG
    AGACAGCTAC ATGAGCTCGC CCCTTCCATC TTCTTCTATT TGGTGGATGC AGAACAGGGA
    CGGCTGTGTG GATACCGACT TCGAAAGGAT CTTACCACTG AGCTAAGTTG GGAGCTGACT
    ATTCCCCCAG AAGTCCAGCG CATTGTCAAG GTGAAGGGGA AGCGCAGCAG TGAGCACGTT
    CATTCCCAGG GTCGAGTGAT GGGGGACCGC AGCGTGCTCT ATAAGAGCCT GAACCCCAAC
    CTGCTGGCCG TCGTGACAGA GAGCACAGAC GTACACCATG AGCGCACCTT CATTGGCATC
    TTCCTCGTGG ATGGTGTCAC CGGGCGCATC ATCCATTCCT CCGTGCAGAG GAAGGCCAAG
    GGCCCCGTCC ACATCGTGCA CTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATACCAAG
    GCCCGGCGCA ACGAGTTCAC TGCGCTAGAG CTCTACGAGG GCACCGAGCA GTACAATGCC
    ACGGCCTTCA GTTCCTTGGA CCGCCCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTATATC
    TTCCCCTCCT CCATCAGTGC CATGGAAGCC ACCATCACCG AGCGTGGCAT CACCAGCCGG
    CACCTGCTTA TTGGGCTACC TTCTGGAGCA ATCCTTTCCC TTCCTAAAGC CTTGCTGGAT
    CCCCGCCGTC CCGAGATCCC AACAGAACAA AGCAGAGAGG AGAACCTAAT CCCATATTCC
    CCAGACGTGC AGATACACGC AGAGCGATTC ATTAACTACA ACCAGACGGT TTCTCGAATG
    CGAGGTATCT ACACAGCTCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTATGGT
    TTGGACATTT ACCAAACTCG AGTCTACCCA TCCAAGCAGT TTGATGTCTT GAAGGATGAC
    TATGACTATG TGCTGATCAG CAGTGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACT
    AAGAGACTAG CACAGGTGAA ACTCCTGAAT CGGGCCTGGC GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_855253.2
    CDS18..3002
    Translation

    Target ORF information:

    RefSeq Version XM_850160.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_850160.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGGGAAG
    GTGGTGCTGT GGGGCCGGGA GGAGTCGCTG GCGGAGGTGG TATGCCTGGA GATGGTGGAT
    CTGCCCCTGA CTGGTGCGCA GGCTGAGCTG GAAGGAGAAT TTGGCAAGAA GGCAGACGGC
    TTACTGGGGA TGTTCCTGAA ACGCCTCTCG TCCCAGCTCA TCCTGCTGCA GGCGTGGACT
    TCTCACCTCT GGAAGATGTT TTATGATGCT CGGAAGCCCC GCAGTCAGAT TAAGAATGAG
    ATCAACATTG ACACCCTAGC CAGAGATGAA TTTAATCTCC AGAAGATGAT GGTGATGGTG
    ACAGCCTCGG GCAAGCTTTT TGGCATTGAG AGCAGTTCTG GCACCATCCT GTGGAAACAG
    TATCTCCCCA ATGTCAAGCC AGACTCCTCC TTTAAACTGA TGGTCCAGAG GACTACTGCC
    CATTTCCCCC ACCCCCCGCA GTGCACCCTC CTGGTAAAGG ACAAGGACAC GGGCATGAGT
    TCTCTGTATG TCTTCAATCC CATCTTTGGG AAGTGGAGTC AGGTGGCTCC CCCAGTGCTG
    AAGCGTCCCA TCTTGCAGTC TTTGCTTCTC CCCATCATGG ATCAAGACTA TGCCAAGGTG
    CTGCTGTTGA TCGACGACGA GTATAAGGTC ACAGCGTTCC CAGCCACTCG GAATGTCTTG
    AGACAGCTAC ATGAGCTCGC CCCTTCCATC TTCTTCTATT TGGTGGATGC AGAACAGGGA
    CGGCTGTGTG GATACCGACT TCGAAAGGAT CTTACCACTG AGCTAAGTTG GGAGCTGACT
    ATTCCCCCAG AAGTCCAGCG CATTGTCAAG GTGAAGGGGA AGCGCAGCAG TGAGCACGTT
    CATTCCCAGG GTCGAGTGAT GGGGGACCGC AGCGTGCTCT ATAAGAGCCT GAACCCCAAC
    CTGCTGGCCG TCGTGACAGA GAGCACAGAC GTACACCATG AGCGCACCTT CATTGGCATC
    TTCCTCGTGG ATGGTGTCAC CGGGCGCATC ATCCATTCCT CCGTGCAGAG GAAGGCCAAG
    GGCCCCGTCC ACATCGTGCA CTCAGAGAAC TGGGTGGTGT ACCAGTACTG GAATACCAAG
    GCCCGGCGCA ACGAGTTCAC TGCGCTAGAG CTCTACGAGG GCACCGAGCA GTACAATGCC
    ACGGCCTTCA GTTCCTTGGA CCGCCCCCAG CTGCCCCAGG TCCTCCAGCA GTCCTATATC
    TTCCCCTCCT CCATCAGTGC CATGGAAGCC ACCATCACCG AGCGTGGCAT CACCAGCCGG
    CACCTGCTTA TTGGGCTACC TTCTGGAGCA ATCCTTTCCC TTCCTAAAGC CTTGCTGGAT
    CCCCGCCGTC CCGAGATCCC AACAGAACAA AGCAGAGAGG AGAACCTAAT CCCATATTCC
    CCAGACGTGC AGATACACGC AGAGCGATTC ATTAACTACA ACCAGACGGT TTCTCGAATG
    CGAGGTATCT ACACAGCTCC CTCAGGCCTG GAGTCCACTT GTTTGGTTGT GGCCTATGGT
    TTGGACATTT ACCAAACTCG AGTCTACCCA TCCAAGCAGT TTGATGTCTT GAAGGATGAC
    TATGACTATG TGCTGATCAG CAGTGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACT
    AAGAGACTAG CACAGGTGAA ACTCCTGAAT CGGGCCTGGC GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03718
    Clone ID Related Accession (Same CDS sequence) XM_005617893.2 , XM_005617893.3
    Accession Version XM_005617893.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ER membrane protein complex subunit 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGCA
    ATTCAAGACG GCTTACTGGG GATGTTCCTG AAACGCCTCT CGTCCCAGCT CATCCTGCTG
    CAGGCGTGGA CTTCTCACCT CTGGAAGATG TTTTATGATG CTCGGAAGCC CCGCAGTCAG
    ATTAAGAATG AGATCAACAT TGACACCCTA GCCAGAGATG AATTTAATCT CCAGAAGATG
    ATGGTGATGG TGACAGCCTC GGGCAAGCTT TTTGGCATTG AGAGCAGTTC TGGCACCATC
    CTGTGGAAAC AGTATCTCCC CAATGTCAAG CCAGACTCCT CCTTTAAACT GATGGTCCAG
    AGGACTACTG CCCATTTCCC CCACCCCCCG CAGTGCACCC TCCTGGTAAA GGACAAGGAC
    ACGGGCATGA GTTCTCTGTA TGTCTTCAAT CCCATCTTTG GGAAGTGGAG TCAGGTGGCT
    CCCCCAGTGC TGAAGCGTCC CATCTTGCAG TCTTTGCTTC TCCCCATCAT GGATCAAGAC
    TATGCCAAGG TGCTGCTGTT GATCGACGAC GAGTATAAGG ATCTTACCAC TGAGCTAAGT
    TGGGAGCTGA CTATTCCCCC AGAAGTCCAG CGCATTGTCA AGGTGAAGGG GAAGCGCAGC
    AGTGAGCACG TTCATTCCCA GGGTCGAGTG ATGGGGGACC GCAGCGTGCT CTATAAGAGC
    CTGAACCCCA ACCTGCTGGC CGTCGTGACA GAGAGCACAG ACGTACACCA TGAGCGCACC
    TTCATTGGCA TCTTCCTCGT GGATGGTGTC ACCGGGCGCA TCATCCATTC CTCCGTGCAG
    AGGAAGGCCA AGGGCCCCGT CCACATCGTG CACTCAGAGA ACTGGGTGGT GTACCAGTAC
    TGGAATACCA AGGCCCGGCG CAACGAGTTC ACTGCGCTAG AGCTCTACGA GGGCACCGAG
    CAGTACAATG CCACGGCCTT CAGTTCCTTG GACCGCCCCC AGCTGCCCCA GGTCCTCCAG
    CAGTCCTATA TCTTCCCCTC CTCCATCAGT GCCATGGAAG CCACCATCAC CGAGCGTGGC
    ATCACCAGCC GGCACCTGCT TATTGGGCTA CCTTCTGGAG CAATCCTTTC CCTTCCTAAA
    GCCTTGCTGG ATCCCCGCCG TCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACCTA
    ATCCCATATT CCCCAGACGT GCAGATACAC GCAGAGCGAT TCATTAACTA CAACCAGACG
    GTTTCTCGAA TGCGAGGTAT CTACACAGCT CCCTCAGGCC TGGAGTCCAC TTGTTTGGTT
    GTGGCCTATG GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGATGTC
    TTGAAGGATG ACTATGACTA TGTGCTGATC AGCAGTGTCC TCTTTGGCCT GGTCTTTGCC
    ACCATGATCA CTAAGAGACT AGCACAGGTG AAACTCCTGA ATCGGGCCTG GCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617950.1
    CDS18..2894
    Translation

    Target ORF information:

    RefSeq Version XM_005617893.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617893.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGCA
    ATTCAAGACG GCTTACTGGG GATGTTCCTG AAACGCCTCT CGTCCCAGCT CATCCTGCTG
    CAGGCGTGGA CTTCTCACCT CTGGAAGATG TTTTATGATG CTCGGAAGCC CCGCAGTCAG
    ATTAAGAATG AGATCAACAT TGACACCCTA GCCAGAGATG AATTTAATCT CCAGAAGATG
    ATGGTGATGG TGACAGCCTC GGGCAAGCTT TTTGGCATTG AGAGCAGTTC TGGCACCATC
    CTGTGGAAAC AGTATCTCCC CAATGTCAAG CCAGACTCCT CCTTTAAACT GATGGTCCAG
    AGGACTACTG CCCATTTCCC CCACCCCCCG CAGTGCACCC TCCTGGTAAA GGACAAGGAC
    ACGGGCATGA GTTCTCTGTA TGTCTTCAAT CCCATCTTTG GGAAGTGGAG TCAGGTGGCT
    CCCCCAGTGC TGAAGCGTCC CATCTTGCAG TCTTTGCTTC TCCCCATCAT GGATCAAGAC
    TATGCCAAGG TGCTGCTGTT GATCGACGAC GAGTATAAGG ATCTTACCAC TGAGCTAAGT
    TGGGAGCTGA CTATTCCCCC AGAAGTCCAG CGCATTGTCA AGGTGAAGGG GAAGCGCAGC
    AGTGAGCACG TTCATTCCCA GGGTCGAGTG ATGGGGGACC GCAGCGTGCT CTATAAGAGC
    CTGAACCCCA ACCTGCTGGC CGTCGTGACA GAGAGCACAG ACGTACACCA TGAGCGCACC
    TTCATTGGCA TCTTCCTCGT GGATGGTGTC ACCGGGCGCA TCATCCATTC CTCCGTGCAG
    AGGAAGGCCA AGGGCCCCGT CCACATCGTG CACTCAGAGA ACTGGGTGGT GTACCAGTAC
    TGGAATACCA AGGCCCGGCG CAACGAGTTC ACTGCGCTAG AGCTCTACGA GGGCACCGAG
    CAGTACAATG CCACGGCCTT CAGTTCCTTG GACCGCCCCC AGCTGCCCCA GGTCCTCCAG
    CAGTCCTATA TCTTCCCCTC CTCCATCAGT GCCATGGAAG CCACCATCAC CGAGCGTGGC
    ATCACCAGCC GGCACCTGCT TATTGGGCTA CCTTCTGGAG CAATCCTTTC CCTTCCTAAA
    GCCTTGCTGG ATCCCCGCCG TCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACCTA
    ATCCCATATT CCCCAGACGT GCAGATACAC GCAGAGCGAT TCATTAACTA CAACCAGACG
    GTTTCTCGAA TGCGAGGTAT CTACACAGCT CCCTCAGGCC TGGAGTCCAC TTGTTTGGTT
    GTGGCCTATG GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGATGTC
    TTGAAGGATG ACTATGACTA TGTGCTGATC AGCAGTGTCC TCTTTGGCCT GGTCTTTGCC
    ACCATGATCA CTAAGAGACT AGCACAGGTG AAACTCCTGA ATCGGGCCTG GCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03718
    Clone ID Related Accession (Same CDS sequence) XM_005617893.2 , XM_005617893.3
    Accession Version XM_005617893.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ER membrane protein complex subunit 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGCA
    ATTCAAGACG GCTTACTGGG GATGTTCCTG AAACGCCTCT CGTCCCAGCT CATCCTGCTG
    CAGGCGTGGA CTTCTCACCT CTGGAAGATG TTTTATGATG CTCGGAAGCC CCGCAGTCAG
    ATTAAGAATG AGATCAACAT TGACACCCTA GCCAGAGATG AATTTAATCT CCAGAAGATG
    ATGGTGATGG TGACAGCCTC GGGCAAGCTT TTTGGCATTG AGAGCAGTTC TGGCACCATC
    CTGTGGAAAC AGTATCTCCC CAATGTCAAG CCAGACTCCT CCTTTAAACT GATGGTCCAG
    AGGACTACTG CCCATTTCCC CCACCCCCCG CAGTGCACCC TCCTGGTAAA GGACAAGGAC
    ACGGGCATGA GTTCTCTGTA TGTCTTCAAT CCCATCTTTG GGAAGTGGAG TCAGGTGGCT
    CCCCCAGTGC TGAAGCGTCC CATCTTGCAG TCTTTGCTTC TCCCCATCAT GGATCAAGAC
    TATGCCAAGG TGCTGCTGTT GATCGACGAC GAGTATAAGG ATCTTACCAC TGAGCTAAGT
    TGGGAGCTGA CTATTCCCCC AGAAGTCCAG CGCATTGTCA AGGTGAAGGG GAAGCGCAGC
    AGTGAGCACG TTCATTCCCA GGGTCGAGTG ATGGGGGACC GCAGCGTGCT CTATAAGAGC
    CTGAACCCCA ACCTGCTGGC CGTCGTGACA GAGAGCACAG ACGTACACCA TGAGCGCACC
    TTCATTGGCA TCTTCCTCGT GGATGGTGTC ACCGGGCGCA TCATCCATTC CTCCGTGCAG
    AGGAAGGCCA AGGGCCCCGT CCACATCGTG CACTCAGAGA ACTGGGTGGT GTACCAGTAC
    TGGAATACCA AGGCCCGGCG CAACGAGTTC ACTGCGCTAG AGCTCTACGA GGGCACCGAG
    CAGTACAATG CCACGGCCTT CAGTTCCTTG GACCGCCCCC AGCTGCCCCA GGTCCTCCAG
    CAGTCCTATA TCTTCCCCTC CTCCATCAGT GCCATGGAAG CCACCATCAC CGAGCGTGGC
    ATCACCAGCC GGCACCTGCT TATTGGGCTA CCTTCTGGAG CAATCCTTTC CCTTCCTAAA
    GCCTTGCTGG ATCCCCGCCG TCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACCTA
    ATCCCATATT CCCCAGACGT GCAGATACAC GCAGAGCGAT TCATTAACTA CAACCAGACG
    GTTTCTCGAA TGCGAGGTAT CTACACAGCT CCCTCAGGCC TGGAGTCCAC TTGTTTGGTT
    GTGGCCTATG GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGATGTC
    TTGAAGGATG ACTATGACTA TGTGCTGATC AGCAGTGTCC TCTTTGGCCT GGTCTTTGCC
    ACCATGATCA CTAAGAGACT AGCACAGGTG AAACTCCTGA ATCGGGCCTG GCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617950.1
    CDS32..2908
    Translation

    Target ORF information:

    RefSeq Version XM_005617893.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ER membrane protein complex subunit 1 (EMC1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617893.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGGCGG CGGCGGCGGC CGGTCTCCGC TACTGGCTCT GGGCCGCCCT GCTTGTCCTT 
    GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTCGACT GGAGACAGCA GTATGTCGGG
    AAACTCAAGT TTGCCTCCTT GGAGTTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCTACA
    GAGAAGAATG TGATTGCAGC ATTAAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT
    GACAAGGGCA CAGCAGAAGG GGCAGTGGAT GCTATGCTGC TCTACGGACA GGATGCAATC
    ACTGTGTCCA ATGGAGGCCG GATCATGCGT TCCTGGGAGA CTAACATTGG GGGTCTGAAC
    TGGGAGATTA CCTTGGACAG TGGCAGTTTC CAGGCACTTG GGCTGGTGGG TCTGCAGGAA
    TCAGTGAGGT ACATTGCGGT CCTGAAGAAG ACCACTCTTG CCCTGCATCA TCTCTCCAGC
    GGACACCTGA AGTGGGTGGA ACACCTCCCC GAAAGTGACA GCATCCATTA CCAGATGATG
    TATTCCTACG GTTCTGGGGT GGTGTGGGCC CTTGGAGTTG TTCCCTTCAG CCACGTGAAC
    ATTGTCAAGT TTAATGTGGA AGATGGAGAG ATTGTTCAGC AGGTCAGGGT GTCAACCCCC
    TGGCTTCAGA GTCTTACTGG AGCCTGTGGT GTGGTGGATG AGGCTGTCCT GGTGTGCCCT
    GACCCAAGCT CACGGTCCCT CCAGACCTTG GCCCTGGAGA CAGAGTGGGA GTTGAGGCAG
    ATACCACTGC AGTCTCTGGA TTTAGAATTT GCAAGTGGAT TCCAACCCCG GGTCCTTCCC
    ACACAGCCCA ACCCAGTGGA TCCTTCTCGG GCCCAGTTCT TCCTGCAGTT GTCCCCAAGC
    CACTATGCTC TGCTGCACTA CCGTCACGGA GCCCTGAGTC TGCTCAAAAA CTTCCCACAG
    GCTGCGCTGG TGAGCTTTGC CACCACTGGA GAGAAGACGG TGGCTGCAGT GGTGACCTGT
    CGCAGCGAAG TGCAGAAGCC TAGCAGTTCT GAAGATGGGT CACTGGGGAG CTTTGCGGAA
    AAGCCCAGTC CCCAGGACTC ATTGACTTGC TTCAACCAGA CCTACACCAT TAATCTCTAC
    TTAGTGGAGA CCGGTCGGCG GTTGCTCGAC ACCTCCATCA CCTTCAGTTT GGAACAGAAC
    GGCACTCGGC CAGAGCGGCT CTACATCCAG GTGTTCTTGA AGAAGGACGA CTCTGTGGGC
    TACCGGGCCT TGGTACAGAC AGAGGATCAC CTGCTCCTCT TCCTGCAGCA GCTGGCAGGG
    AAGGTGGTGC TGTGGGGCCG GGAGGAGTCG CTGGCGGAGG TGGTATGCCT GGAGATGGTG
    GATCTGCCCC TGACTGGTGC GCAGGCTGAG CTGGAAGGAG AATTTGGCAA GAAGGCAGCA
    ATTCAAGACG GCTTACTGGG GATGTTCCTG AAACGCCTCT CGTCCCAGCT CATCCTGCTG
    CAGGCGTGGA CTTCTCACCT CTGGAAGATG TTTTATGATG CTCGGAAGCC CCGCAGTCAG
    ATTAAGAATG AGATCAACAT TGACACCCTA GCCAGAGATG AATTTAATCT CCAGAAGATG
    ATGGTGATGG TGACAGCCTC GGGCAAGCTT TTTGGCATTG AGAGCAGTTC TGGCACCATC
    CTGTGGAAAC AGTATCTCCC CAATGTCAAG CCAGACTCCT CCTTTAAACT GATGGTCCAG
    AGGACTACTG CCCATTTCCC CCACCCCCCG CAGTGCACCC TCCTGGTAAA GGACAAGGAC
    ACGGGCATGA GTTCTCTGTA TGTCTTCAAT CCCATCTTTG GGAAGTGGAG TCAGGTGGCT
    CCCCCAGTGC TGAAGCGTCC CATCTTGCAG TCTTTGCTTC TCCCCATCAT GGATCAAGAC
    TATGCCAAGG TGCTGCTGTT GATCGACGAC GAGTATAAGG ATCTTACCAC TGAGCTAAGT
    TGGGAGCTGA CTATTCCCCC AGAAGTCCAG CGCATTGTCA AGGTGAAGGG GAAGCGCAGC
    AGTGAGCACG TTCATTCCCA GGGTCGAGTG ATGGGGGACC GCAGCGTGCT CTATAAGAGC
    CTGAACCCCA ACCTGCTGGC CGTCGTGACA GAGAGCACAG ACGTACACCA TGAGCGCACC
    TTCATTGGCA TCTTCCTCGT GGATGGTGTC ACCGGGCGCA TCATCCATTC CTCCGTGCAG
    AGGAAGGCCA AGGGCCCCGT CCACATCGTG CACTCAGAGA ACTGGGTGGT GTACCAGTAC
    TGGAATACCA AGGCCCGGCG CAACGAGTTC ACTGCGCTAG AGCTCTACGA GGGCACCGAG
    CAGTACAATG CCACGGCCTT CAGTTCCTTG GACCGCCCCC AGCTGCCCCA GGTCCTCCAG
    CAGTCCTATA TCTTCCCCTC CTCCATCAGT GCCATGGAAG CCACCATCAC CGAGCGTGGC
    ATCACCAGCC GGCACCTGCT TATTGGGCTA CCTTCTGGAG CAATCCTTTC CCTTCCTAAA
    GCCTTGCTGG ATCCCCGCCG TCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACCTA
    ATCCCATATT CCCCAGACGT GCAGATACAC GCAGAGCGAT TCATTAACTA CAACCAGACG
    GTTTCTCGAA TGCGAGGTAT CTACACAGCT CCCTCAGGCC TGGAGTCCAC TTGTTTGGTT
    GTGGCCTATG GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGATGTC
    TTGAAGGATG ACTATGACTA TGTGCTGATC AGCAGTGTCC TCTTTGGCCT GGTCTTTGCC
    ACCATGATCA CTAAGAGACT AGCACAGGTG AAACTCCTGA ATCGGGCCTG GCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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