NELFA cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	NELFA
Entrez Gene ID	100464055
Full Name	negative elongation factor complex member A
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019796754.1	XP_019652313.1	LOW QUALITY PROTEIN: negative elongation factor A

Pathways from BioSystems
Gene Ontology

The following NELFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc90867	XM_019796754.1 Latest version!	Ailuropoda melanoleuca negative elongation factor complex member A (NELFA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELFA ( XM_019796754.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019652313.1 Ailuropoda melanoleuca negative elongation factor complex member A (NELFA), mRNA.

Price & Availability↑

CloneID	OAc90867
Clone ID Related Accession (Same CDS sequence)	XM_019796754.1
Accession Version	XM_019796754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	LOW QUALITY PROTEIN: negative elongation factor A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217437.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGAAGGGCG CCCTGGCGGA GATTATCCAG CTCGCCACCC TGGACTCGGA CCCCTGGGTT 
CTCATGGTAG CTGACATCCT GAAGTCCTTT CCCGACACAG GCTCGCTTAA CCTTGACCTC 
GAAGAGCAGA ATCCCAACGT TCAAGATATT TTAGGAGAAC TTAGAGAAAA GGTGAACGAG 
TGTGAAGCGT CCGCCATGCT GCCACTGGAG TGCCAGTACT TGAATAAAAA CGCCCTGACG 
ACCCTCGCCG GACCCCTCAC TCCCCCAGTG AAACATTTCC AGTTAAAAAG GAAACCGAAG 
AGCGCCACGC TGAGGGCAGA GCTCCTGCAG AAATCTACCG AGACGGCCCA GCAGCTGAAG 
AGGAGCGCGG GGGTGCCCTT CCACGCCAAG GGCCGGGGGC TGCTCCGGAA GATGGACACC 
ACAACCCCGC TCAAAGGCAT CCCGAAGCAG GCACCCTTCA GAAGTCCCAC CACCCCCAGT 
GTCTTCAGCC CTGCCGGGAA CCGGACCCCC ATCCCACCTT CGAGGACTCC TCTGCGGAAG 
GAGAGGGGAG TGAAGCTGCT TGACATTTCT GAGCTGGATA TGGTGGGTGC CGGCCGAGAG 
GCAAAGAGGA GGAGGAAGAC GCTGGATGCG GAAGTGGTGG AAAAGCCGGC CAAGGAGGAG 
ACCGTCGTGG AGAACGCCAC CCCCGATTAC GCAGCTGGCC TGGTGTCCAC ACAGAAACTT 
GGGTCCTTGA ACAGCGAGCC CGCGCTGCCC TCCACAAGCT ACCTGCCCGC CACACCCAGC 
GCGGTCCCGG CCTCGTCCTA CGTCCCCAGC TCCGAGAGCC CGGCAGCACC GTCTTCTCGG 
GAAGCCGGCC TGNCNNNNNN NNCGGAGGAA GCCAGTACCC CCAGCCCCGC GCTGCCGGCG 
CAGTTCAAGC AGAGGGCCCC CATGTACAAC AGCGGCCCAA GCCCAGCCGC ATCTGCGCCT 
TCGACGCCCA CCTCTCCCCT GACACCCACC ACACCCCCAG CCGTCACCCC GGCCGCCCAG 
ACACCTCCGG TGGCCATGGT GGCCCCACAG ACCCAGCCCC CTGCCCAGCA GCAGCCCAAG 
AAGAACCTGT CCCTCACGAG AGAGCAGATG TTCGCTGCGC AGGAGATGTT CAAGACGGCC 
AACAAAGTCA CACGGCCCGA GAAGGCCCTC ATCCTGGGCT TCATGGCCGG CTCCCGAGAG 
AACCCGTGCC AGGAGCAGGG CGACGTGATC CAGATCAAGC TCAGCGAGCA CACGGAGGAC 
CTGCCCAAGT CGGACGGCCA GGGCAGCACC ACCATGCTGG TGGACACCGT GTTCGAGATG 
AACTATGCCA CGGGCCAGTG GACGCGCTTC AAGAAGTACA AGCCCATGGC CAACGTGTCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019652313.1
CDS	26..1408
Translation	MKGALAEIIQLATLDSDPWVLMVADILKSFPDTGSLNLDLEEQNPNVQDILGELREKVNECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTTPSVFSPAGNRTPIPPSRTPLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNSEPALPSTSYLPATPSAVPASSYVPSSESPAAPSSREAGLnullnullnullnullEEASTPSPALPAQFKQRAPMYNSGPSPAASAPSTPTSPLTPTTPPAVTPAAQTPPVAMVAPQTQPPAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLPKSDGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMANVS

Target ORF information:

RefSeq Version	XM_019796754.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca negative elongation factor complex member A (NELFA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019796754.1

ORF Insert Sequence:

ATGAAGGGCG CCCTGGCGGA GATTATCCAG CTCGCCACCC TGGACTCGGA CCCCTGGGTT 
CTCATGGTAG CTGACATCCT GAAGTCCTTT CCCGACACAG GCTCGCTTAA CCTTGACCTC 
GAAGAGCAGA ATCCCAACGT TCAAGATATT TTAGGAGAAC TTAGAGAAAA GGTGAACGAG 
TGTGAAGCGT CCGCCATGCT GCCACTGGAG TGCCAGTACT TGAATAAAAA CGCCCTGACG 
ACCCTCGCCG GACCCCTCAC TCCCCCAGTG AAACATTTCC AGTTAAAAAG GAAACCGAAG 
AGCGCCACGC TGAGGGCAGA GCTCCTGCAG AAATCTACCG AGACGGCCCA GCAGCTGAAG 
AGGAGCGCGG GGGTGCCCTT CCACGCCAAG GGCCGGGGGC TGCTCCGGAA GATGGACACC 
ACAACCCCGC TCAAAGGCAT CCCGAAGCAG GCACCCTTCA GAAGTCCCAC CACCCCCAGT 
GTCTTCAGCC CTGCCGGGAA CCGGACCCCC ATCCCACCTT CGAGGACTCC TCTGCGGAAG 
GAGAGGGGAG TGAAGCTGCT TGACATTTCT GAGCTGGATA TGGTGGGTGC CGGCCGAGAG 
GCAAAGAGGA GGAGGAAGAC GCTGGATGCG GAAGTGGTGG AAAAGCCGGC CAAGGAGGAG 
ACCGTCGTGG AGAACGCCAC CCCCGATTAC GCAGCTGGCC TGGTGTCCAC ACAGAAACTT 
GGGTCCTTGA ACAGCGAGCC CGCGCTGCCC TCCACAAGCT ACCTGCCCGC CACACCCAGC 
GCGGTCCCGG CCTCGTCCTA CGTCCCCAGC TCCGAGAGCC CGGCAGCACC GTCTTCTCGG 
GAAGCCGGCC TGNCNNNNNN NNCGGAGGAA GCCAGTACCC CCAGCCCCGC GCTGCCGGCG 
CAGTTCAAGC AGAGGGCCCC CATGTACAAC AGCGGCCCAA GCCCAGCCGC ATCTGCGCCT 
TCGACGCCCA CCTCTCCCCT GACACCCACC ACACCCCCAG CCGTCACCCC GGCCGCCCAG 
ACACCTCCGG TGGCCATGGT GGCCCCACAG ACCCAGCCCC CTGCCCAGCA GCAGCCCAAG 
AAGAACCTGT CCCTCACGAG AGAGCAGATG TTCGCTGCGC AGGAGATGTT CAAGACGGCC 
AACAAAGTCA CACGGCCCGA GAAGGCCCTC ATCCTGGGCT TCATGGCCGG CTCCCGAGAG 
AACCCGTGCC AGGAGCAGGG CGACGTGATC CAGATCAAGC TCAGCGAGCA CACGGAGGAC 
CTGCCCAAGT CGGACGGCCA GGGCAGCACC ACCATGCTGG TGGACACCGT GTTCGAGATG 
AACTATGCCA CGGGCCAGTG GACGCGCTTC AAGAAGTACA AGCCCATGGC CAACGTGTCC 
TAG