TRMT12 cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMT12
Entrez Gene ID	100468536
Full Name	tRNA methyltransferase 12 homolog
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019809863.1	XP_019665422.1	tRNA wybutosine-synthesizing protein 2 homolog

Pathways from BioSystems
Gene Ontology

The following TRMT12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc99515	XM_019809863.1 Latest version!	Ailuropoda melanoleuca tRNA methyltransferase 12 homolog (TRMT12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMT12 ( XM_019809863.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019665422.1 Ailuropoda melanoleuca tRNA methyltransferase 12 homolog (TRMT12), mRNA.

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CloneID	OAc99515
Clone ID Related Accession (Same CDS sequence)	XM_019809863.1
Accession Version	XM_019809863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	tRNA wybutosine-synthesizing protein 2 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003219020.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGG AAGGTGGGAA GCCCGCGGCT GTCGTCGCGG TTGTGACTGA GTCTCGGTTT 
ACCCAGCGAT ACAGGGAATA TCTCGAGAAG CAGAAACTCC TGGATAGACA GCACCGTGTA 
GAAAAGATGC CGGATGGCAC GGTGGCGCTG CCCGTGGTGG GAGAGGCTCT CCCTGAGCAG 
TACCTGCAGG AGCTGAGGGA TCGTGTGGCT CCAGGCAGCA CCTGTAAAGT AACACAGCTC 
CTGGATCCTG TTCCCTCAAA GAAGGCCCAG GGTTGTTCAC CCGCCCAAAG GCTGTGTCTT 
GAAGTGAGTC GCTGGGTAGA GGGCCGGGGA GTGACGTGGT CAGCCGAGTT GGAGGCTGAC 
TTGCCCCGAT CTTGGCAACG ACATGGTGAC CTCTTGTTGC TAAGCGAGGA CTGTTTCCAA 
GCCAAGCAAT GGAAAAATCT GGAACCAGAA CTCTGGGAGG CTGTTGCGTC GGCACTTGGC 
GTCCATCGTG TGGCTAAACG AGGGCGGGTA TCACCAGATG GCACTCGAAC ACCAGCAGTG 
ACTCTGCTGC TGGGCGCCCA TGGCTGGGTA GAGCATGTGG ATAATGGGAT CCGATATGAG 
TTTGACGTGA CCCGGTGCAT GTTCTCCTTC GGAAACATCA CTGAGAAACT TCGAGTGGCA 
TCGCTGCCCT GTGCTGGAGA AGTGCTGGTG GATCTCTATG CAGGGATTGG TTATTTTACG 
TTGCCCTTCC TAGTCCATGC TGGTGCTGCC TTCGTCCATG CCTGTGAGTG GAACCCCCAT 
GCTGTAGTTG CTCTGAGAAA GAACCTTGAT ATCAATGGAG TAGCAGATCG GTGCCAAATA 
CACTTTGGGG ATAACAGAAA ACTGAAGCTC TCAAACATTG CAGACAGGGT GAACCTGGGA 
CTAATTCCTA GTTCTGAAGA AGGCTGGCCC ATTGCCTGCC AAGTGTTAAG ACGGGATGCT 
GGGGGCATTT TGCATATCCA CCAAAATGTG GAATCTTACC CAGGCAAGAA TCTCCAGCCT 
CCTGGAGGCA GTGAAATGGA GAAGGAGCAC TGGCCTTATC CTCAGCAAAT TATCACCAAC 
GAGTGTACAA ATGGAGCAAC CAGGGATTCT AGGAGAAAAA CACTATCAGC TACCACCAAA 
CCAGAGTGGC AGAGGTGGGC GGAATCTGCA GAAATTCGTA TTGCCACCCT TCTTCAGCAG 
GTGCATGGGA CACGGTGGAA GACACAAATC CTGCACATCC AACCAGTGAA ATCCTATGCT 
CCCCACGTGG ATCACATAGT GTTGGATTTG GAATGCCGCC CCTGTCCTCT AGTTGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019665422.1
CDS	477..1796
Translation	MEREGGKPAAVVAVVTESRFTQRYREYLEKQKLLDRQHRVEKMPDGTVALPVVGEALPEQYLQELRDRVAPGSTCKVTQLLDPVPSKKAQGCSPAQRLCLEVSRWVEGRGVTWSAELEADLPRSWQRHGDLLLLSEDCFQAKQWKNLEPELWEAVASALGVHRVAKRGRVSPDGTRTPAVTLLLGAHGWVEHVDNGIRYEFDVTRCMFSFGNITEKLRVASLPCAGEVLVDLYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRKNLDINGVADRCQIHFGDNRKLKLSNIADRVNLGLIPSSEEGWPIACQVLRRDAGGILHIHQNVESYPGKNLQPPGGSEMEKEHWPYPQQIITNECTNGATRDSRRKTLSATTKPEWQRWAESAEIRIATLLQQVHGTRWKTQILHIQPVKSYAPHVDHIVLDLECRPCPLVG

Target ORF information:

RefSeq Version	XM_019809863.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca tRNA methyltransferase 12 homolog (TRMT12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019809863.1

ORF Insert Sequence:

ATGGAAAGGG AAGGTGGGAA GCCCGCGGCT GTCGTCGCGG TTGTGACTGA GTCTCGGTTT 
ACCCAGCGAT ACAGGGAATA TCTCGAGAAG CAGAAACTCC TGGATAGACA GCACCGTGTA 
GAAAAGATGC CGGATGGCAC GGTGGCGCTG CCCGTGGTGG GAGAGGCTCT CCCTGAGCAG 
TACCTGCAGG AGCTGAGGGA TCGTGTGGCT CCAGGCAGCA CCTGTAAAGT AACACAGCTC 
CTGGATCCTG TTCCCTCAAA GAAGGCCCAG GGTTGTTCAC CCGCCCAAAG GCTGTGTCTT 
GAAGTGAGTC GCTGGGTAGA GGGCCGGGGA GTGACGTGGT CAGCCGAGTT GGAGGCTGAC 
TTGCCCCGAT CTTGGCAACG ACATGGTGAC CTCTTGTTGC TAAGCGAGGA CTGTTTCCAA 
GCCAAGCAAT GGAAAAATCT GGAACCAGAA CTCTGGGAGG CTGTTGCGTC GGCACTTGGC 
GTCCATCGTG TGGCTAAACG AGGGCGGGTA TCACCAGATG GCACTCGAAC ACCAGCAGTG 
ACTCTGCTGC TGGGCGCCCA TGGCTGGGTA GAGCATGTGG ATAATGGGAT CCGATATGAG 
TTTGACGTGA CCCGGTGCAT GTTCTCCTTC GGAAACATCA CTGAGAAACT TCGAGTGGCA 
TCGCTGCCCT GTGCTGGAGA AGTGCTGGTG GATCTCTATG CAGGGATTGG TTATTTTACG 
TTGCCCTTCC TAGTCCATGC TGGTGCTGCC TTCGTCCATG CCTGTGAGTG GAACCCCCAT 
GCTGTAGTTG CTCTGAGAAA GAACCTTGAT ATCAATGGAG TAGCAGATCG GTGCCAAATA 
CACTTTGGGG ATAACAGAAA ACTGAAGCTC TCAAACATTG CAGACAGGGT GAACCTGGGA 
CTAATTCCTA GTTCTGAAGA AGGCTGGCCC ATTGCCTGCC AAGTGTTAAG ACGGGATGCT 
GGGGGCATTT TGCATATCCA CCAAAATGTG GAATCTTACC CAGGCAAGAA TCTCCAGCCT 
CCTGGAGGCA GTGAAATGGA GAAGGAGCAC TGGCCTTATC CTCAGCAAAT TATCACCAAC 
GAGTGTACAA ATGGAGCAAC CAGGGATTCT AGGAGAAAAA CACTATCAGC TACCACCAAA 
CCAGAGTGGC AGAGGTGGGC GGAATCTGCA GAAATTCGTA TTGCCACCCT TCTTCAGCAG 
GTGCATGGGA CACGGTGGAA GACACAAATC CTGCACATCC AACCAGTGAA ATCCTATGCT 
CCCCACGTGG ATCACATAGT GTTGGATTTG GAATGCCGCC CCTGTCCTCT AGTTGGGTAG