NEFL cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFL
Entrez Gene ID	100482068
Full Name	neurofilament light
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019794347.1	XP_019649906.1	neurofilament light polypeptide

Pathways from BioSystems
Gene Ontology

The following NEFL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc89353	XM_019794347.1 Latest version!	Ailuropoda melanoleuca neurofilament light (NEFL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFL ( XM_019794347.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019649906.1 Ailuropoda melanoleuca neurofilament light (NEFL), mRNA.

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CloneID	OAc89353
Clone ID Related Accession (Same CDS sequence)	XM_019794347.1
Accession Version	XM_019794347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	neurofilament light polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217339.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCT TCAGCTACGA GCCGTACTAC TCGACCTCCT ACAAGCGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCCGCTTAC 
TCCAGCTACT CCGCGCCGGT CTCCTCCTCG CTGTCCGTGC GCCGAAGCTA CTCCTCCAGC 
TCCGGCTCCT TGATGCCCAG TCTCGAGAAC CTCGACCTGA GCCAGGTAGC CGCTATCAGC 
AACGACCTCA AGTCAATCCG CACCCAGGAG AAAGCGCAGC TGCAGGACCT CAACGACCGC 
TTCGCCAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT GCTGGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCCTCCC GCTTCCGGGC GCTGTACGAG 
CAGGAGATCC GCGACCTGCG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCACTC 
CAGGGCGAGC GCGAAGGGCT GGAGGAGACT TTGCGCAACC TGCAGGCGCG CTATGAAGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGGCTGATGG AAGCGCGCAA AGGAGCCGAC 
GAGGCCGCTC TCGCCCGCGC CGAGCTCGAA AAACGCATTG ACAGCCTGAT GGACGAAATC 
GCCTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCGCA GATCCAGTAC 
GCTCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC TGCGCTCAAG 
GACATCCGTG CTCAGTACGA GAAGCTGGCT GCCAAGAACA TGCAGAACGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACGGT GCTGACCGAG AGCGCCGCCA AGAACACCGA CGCGGTGCGC 
GCCGCCAAGG ACGAGGTGTC CGAGAGCCGC CGCCTGCTCA AGGCCAAGAC CCTGGAGATC 
GAAGCATGCC GGGGCATGAA CGAAGCGCTG GAGAAGCAGC TGCAGGAGCT GGAGGACAAG 
CAGAACGCCG ATATTAGTGC TATGCAGGAC ACAATCAACA AATTAGAAAA TGAATTGCGA 
ACCACGAAGA GTGAAATGGC ACGATACCTT AAAGAATACC AAGACCTCCT CAATGTGAAG 
ATGGCTTTGG ACATTGAGAT TGCAGCTTAT AGGAAACTCT TGGAGGGTGA GGAGACCCGG 
CTCAGTTTTA CCAGCGTGGG AAGCATAACC AGCGGCTACT CCCAGAGCTC TCAGGTCTTT 
GGCCGATCTG CCTATGGTGG GTTACAGACC AGCTCCTACT TGATGTCTGC CCGCTCCTTC 
CCGTCTTATT ACACCAGCCA CGTCCAGGAG GAGCAGATCG AGGTGGAGGA GACCATTGAG 
GCTGCAAAGG CCGAGGAAGC CAAGGACGAA CCTGCCTCTG AAGGAGAAGC CGAGGAGGAG 
GAGAAGGAGA AGGAAGAGGC TGCTGAAGAG GAAGGAGCTG AAGAGGAAGA AGCTGCCAAG 
GAAGAATCTG AGGAGGCAAA GGAGGAAGAG GAAGGAGGTG AAGGCGAAGA AGGAGAAGAA 
ACCAAAGAAG CCGAGGAGGA GGAGAAAAAA GATGACGGTG CTGGGGAGGA ACAAGCAACT 
AAAAAGAAAG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019649906.1
CDS	325..1959
Translation	MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSARSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPASEGEAEEEEKEKEEAAEEEGAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKDDGAGEEQATKKKD

Target ORF information:

RefSeq Version	XM_019794347.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca neurofilament light (NEFL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019794347.1

ORF Insert Sequence:

ATGAGTTCCT TCAGCTACGA GCCGTACTAC TCGACCTCCT ACAAGCGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCACCGCGCG CTCCGCTTAC 
TCCAGCTACT CCGCGCCGGT CTCCTCCTCG CTGTCCGTGC GCCGAAGCTA CTCCTCCAGC 
TCCGGCTCCT TGATGCCCAG TCTCGAGAAC CTCGACCTGA GCCAGGTAGC CGCTATCAGC 
AACGACCTCA AGTCAATCCG CACCCAGGAG AAAGCGCAGC TGCAGGACCT CAACGACCGC 
TTCGCCAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT GCTGGAAGCC 
GAGCTGCTGG TGCTGCGCCA GAAGCACTCC GAGCCCTCCC GCTTCCGGGC GCTGTACGAG 
CAGGAGATCC GCGACCTGCG CCTGGCGGCG GAAGACGCCA CCAACGAGAA GCAGGCACTC 
CAGGGCGAGC GCGAAGGGCT GGAGGAGACT TTGCGCAACC TGCAGGCGCG CTATGAAGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGGCTGATGG AAGCGCGCAA AGGAGCCGAC 
GAGGCCGCTC TCGCCCGCGC CGAGCTCGAA AAACGCATTG ACAGCCTGAT GGACGAAATC 
GCCTTTCTGA AGAAAGTGCA CGAAGAGGAG ATCGCCGAGC TGCAGGCGCA GATCCAGTAC 
GCTCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC TGCGCTCAAG 
GACATCCGTG CTCAGTACGA GAAGCTGGCT GCCAAGAACA TGCAGAACGC CGAAGAGTGG 
TTCAAGAGCC GCTTCACGGT GCTGACCGAG AGCGCCGCCA AGAACACCGA CGCGGTGCGC 
GCCGCCAAGG ACGAGGTGTC CGAGAGCCGC CGCCTGCTCA AGGCCAAGAC CCTGGAGATC 
GAAGCATGCC GGGGCATGAA CGAAGCGCTG GAGAAGCAGC TGCAGGAGCT GGAGGACAAG 
CAGAACGCCG ATATTAGTGC TATGCAGGAC ACAATCAACA AATTAGAAAA TGAATTGCGA 
ACCACGAAGA GTGAAATGGC ACGATACCTT AAAGAATACC AAGACCTCCT CAATGTGAAG 
ATGGCTTTGG ACATTGAGAT TGCAGCTTAT AGGAAACTCT TGGAGGGTGA GGAGACCCGG 
CTCAGTTTTA CCAGCGTGGG AAGCATAACC AGCGGCTACT CCCAGAGCTC TCAGGTCTTT 
GGCCGATCTG CCTATGGTGG GTTACAGACC AGCTCCTACT TGATGTCTGC CCGCTCCTTC 
CCGTCTTATT ACACCAGCCA CGTCCAGGAG GAGCAGATCG AGGTGGAGGA GACCATTGAG 
GCTGCAAAGG CCGAGGAAGC CAAGGACGAA CCTGCCTCTG AAGGAGAAGC CGAGGAGGAG 
GAGAAGGAGA AGGAAGAGGC TGCTGAAGAG GAAGGAGCTG AAGAGGAAGA AGCTGCCAAG 
GAAGAATCTG AGGAGGCAAA GGAGGAAGAG GAAGGAGGTG AAGGCGAAGA AGGAGAAGAA 
ACCAAAGAAG CCGAGGAGGA GGAGAAAAAA GATGACGGTG CTGGGGAGGA ACAAGCAACT 
AAAAAGAAAG ATTGA