LOC100560101 cDNA ORF clone, Anolis carolinensis(green anole)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100560101
Entrez Gene ID	100560101
Full Name	nucleolar protein 58
Gene Type	protein-coding
Organism	Anolis carolinensis(green anole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003230328.2	XP_003230376.2	nucleolar protein 58

Pathways from BioSystems
Gene Ontology

The following LOC100560101 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100560101 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn22023	XM_003230328.2 Latest version!	Anolis carolinensis nucleolar protein 58 (LOC100560101), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100560101 ( XM_003230328.2 ) cDNA ORF clone, Anolis carolinensis(green anole) -> XP_003230376.2 Anolis carolinensis nucleolar protein 58 (LOC100560101), mRNA.

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CloneID	OAn22023
Clone ID Related Accession (Same CDS sequence)	XM_003230328.2
Accession Version	XM_003230328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-30
Organism	Anolis carolinensis(green anole)
Product	nucleolar protein 58
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003340562.1) and transcript sequence (GAFK01002415.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2014 this sequence version replaced XM_003230328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Anolis carolinensis Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1451 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGGTTC TCTTCGAGAC GGCGGCCGGG TACGCCATTT TCAAGGTTCT GAATGAGAAG 
AAGCTCCAAG AAATTGATAG TTTATGGAAA GAGTTCGAAA CTCCTGAAAA AGCTAATAAA 
ATTGTGAAAT TGAAACACTT TGAGAAGTTT CAGGACACTA CAGAAGCACT TGCAGCATCG 
ACAGCTCTGG TGGAAGGCAA GATTAGCAAG AATTTTAAAA AAATCTTAAA GAAGATAGTC 
GCCAAAGATG CCCATGAACA GTTGGCTGTG GCAGATGCCA AACTGGGTGG TGTCATAAAG 
GACAAGCTGA ACTTGAGCTG TATCCACAGC CCAATGGTCA CTGAACTGAT GAGAGGCATT 
CGTACACAAA TGGAAGGTCT TATCAGTGGT CTTCCAGCAA AAGAGATGGC AGCTATGTGT 
TTAGGATTGG CACATAGCCT CTCCCGATAC AAGTTGAAGT TCAGTCCAGA CAAAGTTGAC 
ACCATGATTG TCCAAGCAAT CTCCCTTCTT GATGACTTGG ACAAAGAACT AAATAACTAC 
ATCATGCGCT GTAGAGAATG GTATGGTTGG CACTTTCCAG AACTGGGCAA AATTATCACA 
GATAACCTAA CCTACTGTAA ATGTGTACAG GAAATTGGTG ACAGAAAAAA CTTTTCCTGT 
TCTGATCTGT CTGAGATTAT TCCCGAGGAA ATTGAAGAGG AAGTGAAAGC AGCTGCAGAG 
ATCTCCATGG GAACTGAAGT GTCTGAAGAA GATATAAGCA ACATCCAGCA CTTGTGTGAC 
CAGGTTATCG AGATCTCAGA CTATCGAGCA CAGCTATATG ATTATCTAAA AAACAGGATG 
ATGGCCATTG CACCTAATCT TACAGTTATG GTGGGAGAGC TTGTTGGAGC CCGGCTTATT 
GCACATGCAG GTTCCCTTTT GAATCTGGCT AAGCATCCAG CATCAACTGT TCAAATTCTG 
GGTGCCGAAA AAGCACTGTT TAGAGCTTTG AAGACAAAAC ATGACACTCC CAAGTTTGGT 
CTGATTTATC ATGCTTCCCT TGTGGGACAA ACAACAGCCA AAAACAAAGG AAAGATCTCA 
CGAATGCTGG CTGCGAAAAC AGCGTTGACT ATTCGTTATG ATGCCCTTGG AGAAAGTACC 
AGTGCTGAAA TGGGTGCTGA GAACAGATTA AAAGTAGAAG CAAGACTAAG GCACTTGGAA 
GAGAAAGGGA TAAGACGAAT AAGTGGCACA GGAAAAGCTA TGGCCAGGGC AGACAAATAT 
CAACATAAGA GTGAAGTGAA GACGTACGAC CCTTCTGGGG AATCCACCCT TCCTCGGGTC 
CCAAAAAAAC GCAAAATCGA AGCTGAGGAG GAAGAGGAGG TACCAGCTCC CAAGAAAGCA 
AAGAAGACTA AAGTAGTAGA AATTAAAGAA GAGGAGGCAG CAGTAGAGGA TGAGCAAAAT 
GATAGTGGAA GTGTCAAAAA GAAGAAAAAG AAAAAGGATA AAAAACAGGA AGCTCAAGAA 
GAAGCAGTTT GTGTGGAGGA AGATGAAGAA GAGCCATCAA CCAGTACAGC AGTAGAGAAT 
ACAGAGAAGA AGAAAAAGAA GAAGAAGAAA ATGAAAGAGG AGCCTGAAGA TGATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003230376.2
CDS	181..1800
Translation	MLVLFETAAGYAIFKVLNEKKLQEIDSLWKEFETPEKANKIVKLKHFEKFQDTTEALAASTALVEGKISKNFKKILKKIVAKDAHEQLAVADAKLGGVIKDKLNLSCIHSPMVTELMRGIRTQMEGLISGLPAKEMAAMCLGLAHSLSRYKLKFSPDKVDTMIVQAISLLDDLDKELNNYIMRCREWYGWHFPELGKIITDNLTYCKCVQEIGDRKNFSCSDLSEIIPEEIEEEVKAAAEISMGTEVSEEDISNIQHLCDQVIEISDYRAQLYDYLKNRMMAIAPNLTVMVGELVGARLIAHAGSLLNLAKHPASTVQILGAEKALFRALKTKHDTPKFGLIYHASLVGQTTAKNKGKISRMLAAKTALTIRYDALGESTSAEMGAENRLKVEARLRHLEEKGIRRISGTGKAMARADKYQHKSEVKTYDPSGESTLPRVPKKRKIEAEEEEEVPAPKKAKKTKVVEIKEEEAAVEDEQNDSGSVKKKKKKKDKKQEAQEEAVCVEEDEEEPSTSTAVENTEKKKKKKKKMKEEPEDDD

Target ORF information:

RefSeq Version	XM_003230328.2
Organism	Anolis carolinensis(green anole)
Definition	Anolis carolinensis nucleolar protein 58 (LOC100560101), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003230328.2

ORF Insert Sequence:

ATGCTGGTTC TCTTCGAGAC GGCGGCCGGG TACGCCATTT TCAAGGTTCT GAATGAGAAG 
AAGCTCCAAG AAATTGATAG TTTATGGAAA GAGTTCGAAA CTCCTGAAAA AGCTAATAAA 
ATTGTGAAAT TGAAACACTT TGAGAAGTTT CAGGACACTA CAGAAGCACT TGCAGCATCG 
ACAGCTCTGG TGGAAGGCAA GATTAGCAAG AATTTTAAAA AAATCTTAAA GAAGATAGTC 
GCCAAAGATG CCCATGAACA GTTGGCTGTG GCAGATGCCA AACTGGGTGG TGTCATAAAG 
GACAAGCTGA ACTTGAGCTG TATCCACAGC CCAATGGTCA CTGAACTGAT GAGAGGCATT 
CGTACACAAA TGGAAGGTCT TATCAGTGGT CTTCCAGCAA AAGAGATGGC AGCTATGTGT 
TTAGGATTGG CACATAGCCT CTCCCGATAC AAGTTGAAGT TCAGTCCAGA CAAAGTTGAC 
ACCATGATTG TCCAAGCAAT CTCCCTTCTT GATGACTTGG ACAAAGAACT AAATAACTAC 
ATCATGCGCT GTAGAGAATG GTATGGTTGG CACTTTCCAG AACTGGGCAA AATTATCACA 
GATAACCTAA CCTACTGTAA ATGTGTACAG GAAATTGGTG ACAGAAAAAA CTTTTCCTGT 
TCTGATCTGT CTGAGATTAT TCCCGAGGAA ATTGAAGAGG AAGTGAAAGC AGCTGCAGAG 
ATCTCCATGG GAACTGAAGT GTCTGAAGAA GATATAAGCA ACATCCAGCA CTTGTGTGAC 
CAGGTTATCG AGATCTCAGA CTATCGAGCA CAGCTATATG ATTATCTAAA AAACAGGATG 
ATGGCCATTG CACCTAATCT TACAGTTATG GTGGGAGAGC TTGTTGGAGC CCGGCTTATT 
GCACATGCAG GTTCCCTTTT GAATCTGGCT AAGCATCCAG CATCAACTGT TCAAATTCTG 
GGTGCCGAAA AAGCACTGTT TAGAGCTTTG AAGACAAAAC ATGACACTCC CAAGTTTGGT 
CTGATTTATC ATGCTTCCCT TGTGGGACAA ACAACAGCCA AAAACAAAGG AAAGATCTCA 
CGAATGCTGG CTGCGAAAAC AGCGTTGACT ATTCGTTATG ATGCCCTTGG AGAAAGTACC 
AGTGCTGAAA TGGGTGCTGA GAACAGATTA AAAGTAGAAG CAAGACTAAG GCACTTGGAA 
GAGAAAGGGA TAAGACGAAT AAGTGGCACA GGAAAAGCTA TGGCCAGGGC AGACAAATAT 
CAACATAAGA GTGAAGTGAA GACGTACGAC CCTTCTGGGG AATCCACCCT TCCTCGGGTC 
CCAAAAAAAC GCAAAATCGA AGCTGAGGAG GAAGAGGAGG TACCAGCTCC CAAGAAAGCA 
AAGAAGACTA AAGTAGTAGA AATTAAAGAA GAGGAGGCAG CAGTAGAGGA TGAGCAAAAT 
GATAGTGGAA GTGTCAAAAA GAAGAAAAAG AAAAAGGATA AAAAACAGGA AGCTCAAGAA 
GAAGCAGTTT GTGTGGAGGA AGATGAAGAA GAGCCATCAA CCAGTACAGC AGTAGAGAAT 
ACAGAGAAGA AGAAAAAGAA GAAGAAGAAA ATGAAAGAGG AGCCTGAAGA TGATGACTGA