LOC100578862 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100578862
Entrez Gene ID	100578862
Full Name	dynein assembly factor 1, axonemal homolog
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_026445192.1	XP_026300977.1	DNA ligase 1 isoform X2
XM_016916231.1	XP_016771720.1	dynein assembly factor 1, axonemal homolog
XM_026445191.1	XP_026300976.1	DNA ligase 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100578862 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100578862 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd27351	XM_026445191.1 Latest version!	Apis mellifera DNA ligase 1 (LOC100578862), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHd27352	XM_026445192.1 Latest version!	Apis mellifera DNA ligase 1 (LOC100578862), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHd20052	XM_016916231.1 Latest version!	Apis mellifera dynein assembly factor 1, axonemal homolog (LOC100578862), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100578862 ( XM_026445191.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026300976.1 Apis mellifera DNA ligase 1 (LOC100578862), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHd27351
Clone ID Related Accession (Same CDS sequence)	XM_026445191.1
Accession Version	XM_026445191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	DNA ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037651.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGGG GGCCCGAGGA AGAGGCGGCC GAGAGGAAAC GGTGGATCGA GGCGGAGCAG 
AAGAAAATCA ATGATAGCGT GCTCGCCCTG ATAAACAAAA GGAAGCAGTG CAAACCGGTC 
GAGACGTCCG AAAAGGAGGC GGAGGATAAG AAAAGGACGA AGGAGGACGA AGAAGTAGCC 
ACGACCACGG TTGTCTGCAC GAGCGACGAA CTGCTCACTC TGGAGAGGAA GAAAAAGTTG 
GAGGTGTCCT CATCCTCCGA CAGCTCCTCC GCGTCCTCGT CGTCGGGCGA AGAGGAGGAT 
GACGCGGCGA CGCAAAAATC GAGGATCGAG GAAAGCGGCG GCGGAGGAGG GACGGCGGAG 
GAAGGGGGGG AAGAAGCTTC TTCGCCCGAT CGAGGCGACG AAATTTTGTT ACCCTGGAAA 
ACTCAGGTTC CCGCTCGTAG GAGCGTTAAA AAGTTGGTGG AGGTGATAGA GGAGGACGCG 
AAGGAATTCG TGGCCGGCGA TACGATGGGA ATTTTGGAAG AACGAAATGG AAAGATCGAT 
GATCCACCGA CGATCGATCG CGAGATGATT GCAGGGAACG AGAACGAGAT CATTCCATCC 
TGTTCCGAGG AGGAAGGAAA GTTTAAAAGT GTCTCGGTTT CTTGCAACAT TTTGAACACG 
AGGAGCGATG AGAGGAGGAG GGAGGATAAC GTCGAATATC CGTCCAAAGA TATCCTCGAC 
AATTATCGAA GAAAGGACGA TCCGCATCCT TTGAGCGGGC AGTTGAGCAG TATCAGGGAG 
GACATGAAGG AGTTCTGCGT CGAAATGGAC AAATTCGTGG AGAGGAACAA GTTTGTGGTC 
AAAGATAGCG ATAAAACGAA AAAGGGGGAG TCGTCGGTGG GCGAGGAGAA AGATTCGTGC 
AAATGGTGGG ACACGAAGGA GAGGAAGTTG AAGGTGAAAG AGATCTTGAG GCAGAGAGAG 
GAAGCGGAGA GATCGAAAAT TGTCAAGAGG GTCGAAATTG TCGAGGTTGA TAAAGAAGAA 
GAAAAAGAAG AGGAAAAAGG AGAAGAAGAA GAAGAAGAAG AAGAAGCTTC GAGGAATGTT 
AAAGAAGTGG TTAAAGACGA GAAGAACGTT CCATCTTGCC AAGGTGTGTA CGATCTGTTG 
AATCTGAAGA AGTGTCCTGA AATTTTGTTG AAGGATGTGA AAGCTCGGGT TAAAACCGAC 
GAAGACGATG CTGCCACGTT GAAATGCGAA TCCGTTAAAA ACGACAACAG CAAGGATCGC 
CTCTCGGGAT TGTACAATTC GTTGTTCAAC GAGATGAATT ATAGAAGCGG GGTCGAGAAA 
AATCGATCGA GGAAATCGCT CAGTTCCCAA GAATTGTTGA CGTTGGAGAA AATAGACGAA 
ACAGCCGAGG AAACAACGCG GTCCGCGAGT TGTATCGACA TAATTTCCTC GGATGTTGAT 
AATTCAGAGA TGATCAGCGT TAAGAGCGAG CCTGTTGGTA TAAAAATCGT GGAAGCAAGC 
AACGTGACCG CCCTAAATTC CACCGACGAC GAGGTGTCGG ACAGCGAGTC TGTTAAAACG 
ATAATTAACA ATTACGATAA GCCGAGAGGA ATATACGAAG AGGTGAACGC GAGTACGAGC 
CAGGATATGC CAGGGGAGCG AGGGGAAAAT AATTGTGGAT GGGACAAGCC TGATAATGCG 
AAGAAAATTG TCGAAGATAA CGATGAGATC CAATCGTCGG AGGAGAAGGA ATCCGCCAAG 
GTGTCCTCTT CCACGAATAT CGTCGAAAAA TCTTCCAAAT CGCACAAAAG ATCGCGCGAC 
TGCGAATGCC AAGACGTGGC CAGTAAAAAG TTCCACTTGA TCGAGGAGAT GGAAATGCAA 
GAAAATGCAA CAGGCAGCGA AAAGATGGTG GATGGAATAT CTGAAAAATG TAAACGGCAT 
TTCATAAAAG AGGCAAGGGA GTTCGTGAAG AAGGAATCGC CTCTGATAGA TCGGTGCATA 
CAGGATTTGA TAAGTAAAAA ATCGAAGGGG AATTGGGATT TGAAGAATAA TCAAGAGGAA 
TTTTTAACTT GTACAGCTTT GAACATCGCC ACCTCCGACA TATTTTCAAA CAAAAGTTGG 
GAGAATTCGG ATAAGAGAGT CGGGTCAACG TCGAACGAGC AACATCGAGC GATGGAGGCG 
AAAAAATTGT TCGACGTTCA AAATTCTGAA GATAAACCAG AAATTGGAGA TTCATCGATC 
GACAAACAAG TTGATATAAA ATCGGTCGAT CATCTTTTGA AGCAATCCTC AATTGCCGAA 
AAGCAGTCGA AAATGTGCAC GGATCTGTAC AAACAATTCT GCAAACATCT GGAAGAGATA 
GACAGTAAGA GAAAGCTTTT GATCGAGCCG GACTTCATGA AAAAAAATAA ATCGGATCAC 
GAGGAAAAGA AACGTGACGT TCTATCGCCA AGGGAGACCA AACAGTCGAA AGAGGAACAA 
ACAAAATCAT TGATCGAGGT AATTTCGGAG GACGCGACCA CGAGCGTGGA GGAACTCGAA 
GTTGACAAGT TAGTTTTCGA TTCTGATCCT GTGATGGATG CAGAGTTGAA AGATAAAATA 
TTAAAAACAA TTAATACTCC GAAAAGCGAG GAGGAGAGAG AAAAGGGAAA GAGATCGGCC 
GATAAGTTGA TGAAGATTTC CAGGGAAGCT ATGGCCAAGG GGAAATCCTT GCTCGACCCA 
TCTTTCCCTA TCTGCGGTCA GAAAAACTAT TTCAAGGACA GTAGGAGATT TTTCATGAAT 
TTGCTGGAGG ATGATTTTTC TTCCTCGGAA AAAGACGAGG ATCCAACGAA GAATATCGAC 
GAAACGGAAT CGATCATGTT CAGAGAGAAT TCAGTTGCAT TGATAGAAGA AATAGAGAAA 
TTATCGTCGA TCGAAGATAA CGGTATACAG GGTAATAATG TTGTTGATAT TGGAAAAGAA 
AACGTTACGA TTATTTCAGA GAACTCTGTG ATGAAAATAG AAGAAATCGA GAAATTATCA 
CCGACCGAAG GCAACAGTAT TCAAGATGGT GAAATTCTCG ATATGGAAAA AGAAAACGTG 
AACAGAGTGA GGAAAAGTTT GGAGATGCAG ATTGTTCAAG GAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026300976.1
CDS	51..3158
Translation	MRGGPEEEAAERKRWIEAEQKKINDSVLALINKRKQCKPVETSEKEAEDKKRTKEDEEVATTTVVCTSDELLTLERKKKLEVSSSSDSSSASSSSGEEEDDAATQKSRIEESGGGGGTAEEGGEEASSPDRGDEILLPWKTQVPARRSVKKLVEVIEEDAKEFVAGDTMGILEERNGKIDDPPTIDREMIAGNENEIIPSCSEEEGKFKSVSVSCNILNTRSDERRREDNVEYPSKDILDNYRRKDDPHPLSGQLSSIREDMKEFCVEMDKFVERNKFVVKDSDKTKKGESSVGEEKDSCKWWDTKERKLKVKEILRQREEAERSKIVKRVEIVEVDKEEEKEEEKGEEEEEEEEASRNVKEVVKDEKNVPSCQGVYDLLNLKKCPEILLKDVKARVKTDEDDAATLKCESVKNDNSKDRLSGLYNSLFNEMNYRSGVEKNRSRKSLSSQELLTLEKIDETAEETTRSASCIDIISSDVDNSEMISVKSEPVGIKIVEASNVTALNSTDDEVSDSESVKTIINNYDKPRGIYEEVNASTSQDMPGERGENNCGWDKPDNAKKIVEDNDEIQSSEEKESAKVSSSTNIVEKSSKSHKRSRDCECQDVASKKFHLIEEMEMQENATGSEKMVDGISEKCKRHFIKEAREFVKKESPLIDRCIQDLISKKSKGNWDLKNNQEEFLTCTALNIATSDIFSNKSWENSDKRVGSTSNEQHRAMEAKKLFDVQNSEDKPEIGDSSIDKQVDIKSVDHLLKQSSIAEKQSKMCTDLYKQFCKHLEEIDSKRKLLIEPDFMKKNKSDHEEKKRDVLSPRETKQSKEEQTKSLIEVISEDATTSVEELEVDKLVFDSDPVMDAELKDKILKTINTPKSEEEREKGKRSADKLMKISREAMAKGKSLLDPSFPICGQKNYFKDSRRFFMNLLEDDFSSSEKDEDPTKNIDETESIMFRENSVALIEEIEKLSSIEDNGIQGNNVVDIGKENVTIISENSVMKIEEIEKLSPTEGNSIQDGEILDMEKENVNRVRKSLEMQIVQGN

Target ORF information:

RefSeq Version	XM_026445191.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera DNA ligase 1 (LOC100578862), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026445191.1

ORF Insert Sequence:

ATGCGAGGGG GGCCCGAGGA AGAGGCGGCC GAGAGGAAAC GGTGGATCGA GGCGGAGCAG 
AAGAAAATCA ATGATAGCGT GCTCGCCCTG ATAAACAAAA GGAAGCAGTG CAAACCGGTC 
GAGACGTCCG AAAAGGAGGC GGAGGATAAG AAAAGGACGA AGGAGGACGA AGAAGTAGCC 
ACGACCACGG TTGTCTGCAC GAGCGACGAA CTGCTCACTC TGGAGAGGAA GAAAAAGTTG 
GAGGTGTCCT CATCCTCCGA CAGCTCCTCC GCGTCCTCGT CGTCGGGCGA AGAGGAGGAT 
GACGCGGCGA CGCAAAAATC GAGGATCGAG GAAAGCGGCG GCGGAGGAGG GACGGCGGAG 
GAAGGGGGGG AAGAAGCTTC TTCGCCCGAT CGAGGCGACG AAATTTTGTT ACCCTGGAAA 
ACTCAGGTTC CCGCTCGTAG GAGCGTTAAA AAGTTGGTGG AGGTGATAGA GGAGGACGCG 
AAGGAATTCG TGGCCGGCGA TACGATGGGA ATTTTGGAAG AACGAAATGG AAAGATCGAT 
GATCCACCGA CGATCGATCG CGAGATGATT GCAGGGAACG AGAACGAGAT CATTCCATCC 
TGTTCCGAGG AGGAAGGAAA GTTTAAAAGT GTCTCGGTTT CTTGCAACAT TTTGAACACG 
AGGAGCGATG AGAGGAGGAG GGAGGATAAC GTCGAATATC CGTCCAAAGA TATCCTCGAC 
AATTATCGAA GAAAGGACGA TCCGCATCCT TTGAGCGGGC AGTTGAGCAG TATCAGGGAG 
GACATGAAGG AGTTCTGCGT CGAAATGGAC AAATTCGTGG AGAGGAACAA GTTTGTGGTC 
AAAGATAGCG ATAAAACGAA AAAGGGGGAG TCGTCGGTGG GCGAGGAGAA AGATTCGTGC 
AAATGGTGGG ACACGAAGGA GAGGAAGTTG AAGGTGAAAG AGATCTTGAG GCAGAGAGAG 
GAAGCGGAGA GATCGAAAAT TGTCAAGAGG GTCGAAATTG TCGAGGTTGA TAAAGAAGAA 
GAAAAAGAAG AGGAAAAAGG AGAAGAAGAA GAAGAAGAAG AAGAAGCTTC GAGGAATGTT 
AAAGAAGTGG TTAAAGACGA GAAGAACGTT CCATCTTGCC AAGGTGTGTA CGATCTGTTG 
AATCTGAAGA AGTGTCCTGA AATTTTGTTG AAGGATGTGA AAGCTCGGGT TAAAACCGAC 
GAAGACGATG CTGCCACGTT GAAATGCGAA TCCGTTAAAA ACGACAACAG CAAGGATCGC 
CTCTCGGGAT TGTACAATTC GTTGTTCAAC GAGATGAATT ATAGAAGCGG GGTCGAGAAA 
AATCGATCGA GGAAATCGCT CAGTTCCCAA GAATTGTTGA CGTTGGAGAA AATAGACGAA 
ACAGCCGAGG AAACAACGCG GTCCGCGAGT TGTATCGACA TAATTTCCTC GGATGTTGAT 
AATTCAGAGA TGATCAGCGT TAAGAGCGAG CCTGTTGGTA TAAAAATCGT GGAAGCAAGC 
AACGTGACCG CCCTAAATTC CACCGACGAC GAGGTGTCGG ACAGCGAGTC TGTTAAAACG 
ATAATTAACA ATTACGATAA GCCGAGAGGA ATATACGAAG AGGTGAACGC GAGTACGAGC 
CAGGATATGC CAGGGGAGCG AGGGGAAAAT AATTGTGGAT GGGACAAGCC TGATAATGCG 
AAGAAAATTG TCGAAGATAA CGATGAGATC CAATCGTCGG AGGAGAAGGA ATCCGCCAAG 
GTGTCCTCTT CCACGAATAT CGTCGAAAAA TCTTCCAAAT CGCACAAAAG ATCGCGCGAC 
TGCGAATGCC AAGACGTGGC CAGTAAAAAG TTCCACTTGA TCGAGGAGAT GGAAATGCAA 
GAAAATGCAA CAGGCAGCGA AAAGATGGTG GATGGAATAT CTGAAAAATG TAAACGGCAT 
TTCATAAAAG AGGCAAGGGA GTTCGTGAAG AAGGAATCGC CTCTGATAGA TCGGTGCATA 
CAGGATTTGA TAAGTAAAAA ATCGAAGGGG AATTGGGATT TGAAGAATAA TCAAGAGGAA 
TTTTTAACTT GTACAGCTTT GAACATCGCC ACCTCCGACA TATTTTCAAA CAAAAGTTGG 
GAGAATTCGG ATAAGAGAGT CGGGTCAACG TCGAACGAGC AACATCGAGC GATGGAGGCG 
AAAAAATTGT TCGACGTTCA AAATTCTGAA GATAAACCAG AAATTGGAGA TTCATCGATC 
GACAAACAAG TTGATATAAA ATCGGTCGAT CATCTTTTGA AGCAATCCTC AATTGCCGAA 
AAGCAGTCGA AAATGTGCAC GGATCTGTAC AAACAATTCT GCAAACATCT GGAAGAGATA 
GACAGTAAGA GAAAGCTTTT GATCGAGCCG GACTTCATGA AAAAAAATAA ATCGGATCAC 
GAGGAAAAGA AACGTGACGT TCTATCGCCA AGGGAGACCA AACAGTCGAA AGAGGAACAA 
ACAAAATCAT TGATCGAGGT AATTTCGGAG GACGCGACCA CGAGCGTGGA GGAACTCGAA 
GTTGACAAGT TAGTTTTCGA TTCTGATCCT GTGATGGATG CAGAGTTGAA AGATAAAATA 
TTAAAAACAA TTAATACTCC GAAAAGCGAG GAGGAGAGAG AAAAGGGAAA GAGATCGGCC 
GATAAGTTGA TGAAGATTTC CAGGGAAGCT ATGGCCAAGG GGAAATCCTT GCTCGACCCA 
TCTTTCCCTA TCTGCGGTCA GAAAAACTAT TTCAAGGACA GTAGGAGATT TTTCATGAAT 
TTGCTGGAGG ATGATTTTTC TTCCTCGGAA AAAGACGAGG ATCCAACGAA GAATATCGAC 
GAAACGGAAT CGATCATGTT CAGAGAGAAT TCAGTTGCAT TGATAGAAGA AATAGAGAAA 
TTATCGTCGA TCGAAGATAA CGGTATACAG GGTAATAATG TTGTTGATAT TGGAAAAGAA 
AACGTTACGA TTATTTCAGA GAACTCTGTG ATGAAAATAG AAGAAATCGA GAAATTATCA 
CCGACCGAAG GCAACAGTAT TCAAGATGGT GAAATTCTCG ATATGGAAAA AGAAAACGTG 
AACAGAGTGA GGAAAAGTTT GGAGATGCAG ATTGTTCAAG GAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100578862 ( XM_026445192.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026300977.1 Apis mellifera DNA ligase 1 (LOC100578862), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHd27352
Clone ID Related Accession (Same CDS sequence)	XM_026445192.1
Accession Version	XM_026445192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	DNA ligase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037651.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGGG GGCCCGAGGA AGAGGCGGCC GAGAGGAAAC GGTGGATCGA GGCGGAGCAG 
AAGAAAATCA ATGATAGCGT GCTCGCCCTG ATAAACAAAA GGAAGCAGTG CAAACCGGTC 
GAGACGTCCG AAAAGGAGGC GGAGGATAAG AAAAGGACGA AGGAGGACGA AGAAGTAGCC 
ACGACCACGG TTGTCTGCAC GAGCGACGAA CTGCTCACTC TGGAGAGGAA GAAAAAGTTG 
GAGGTGTCCT CATCCTCCGA CAGCTCCTCC GCGTCCTCGT CGTCGGGCGA AGAGGAGGAT 
GACGCGGCGA CGCAAAAATC GAGGATCGAG GAAAGCGGCG GCGGAGGAGG GACGGCGGAG 
GAAGGGGGGG AAGAAGCTTC TTCGCCCGAT CGAGGCGACG AAATTTTGTT ACCCTGGAAA 
ACTCAGGTTC CCGCTCGTAG GAGCGTTAAA AAGTTGGTGG AGGTGATAGA GGAGGACGCG 
AAGGAATTCG TGGCCGGCGA TACGATGGGA ATTTTGGAAG AACGAAATGG AAAGATCGAT 
GATCCACCGA CGATCGATCG CGAGATGATT GCAGGGAACG AGAACGAGAT CATTCCATCC 
TGTTCCGAGG AGGAAGGAAA GTTTAAAAGT GTCTCGGTTT CTTGCAACAT TTTGAACACG 
AGGAGCGATG AGAGGAGGAG GGAGGATAAC GTCGAATATC CGTCCAAAGA TATCCTCGAC 
AATTATCGAA GAAAGGACGA TCCGCATCCT TTGAGCGGGC AGTTGAGCAG TATCAGGGAG 
GACATGAAGG AGTTCTGCGT CGAAATGGAC AAATTCGTGG AGAGGAACAA GTTTGTGGTC 
AAAGATAGCG ATAAAACGAA AAAGGGGGAG TCGTCGGTGG GCGAGGAGAA AGATTCGTGC 
AAATGGTGGG ACACGAAGGA GAGGAAGTTG AAGGTGAAAG AGATCTTGAG GCAGAGAGAG 
GAAGCGGAGA GATCGAAAAT TGTCAAGAGG GTCGAAATTG TCGAGGTTGA TAAAGAAGAA 
GAAAAAGAAG AGGAAAAAGG AGAAGAAGAA GAAGAAGAAG AAGAAGCTTC GAGGAATGTT 
AAAGAAGTGG TTAAAGACGA GAAGAACGTT CCATCTTGCC AAGGTGTGTA CGATCTGTTG 
AATCTGAAGA AGTGTCCTGA AATTTTGTTG AAGGATGTGA AAGCTCGGGT TAAAACCGAC 
GAAGACGATG CTGCCACGTT GAAATGCGAA TCCGTTAAAA ACGACAACAG CAAGGATCGC 
CTCTCGGGAT TGTACAATTC GTTGTTCAAC GAGATGAATT ATAGAAGCGG GGTCGAGAAA 
AATCGATCGA GGAAATCGCT CAGTTCCCAA GAATTGTTGA CGTTGGAGAA AATAGACGAA 
ACAGCCGAGG AAACAACGCG GTCCGCGAGT TGTATCGACA TAATTTCCTC GGATGTTGAT 
AATTCAGAGA TGATCAGCGT TAAGAGCGAG CCTGTTGGTA TAAAAATCGT GGAAGCAAGC 
AACGTGACCG CCCTAAATTC CACCGACGAC GAGGTGTCGG ACAGCGAGTC TGTTAAAACG 
ATAATTAACA ATTACGATAA GCCGAGAGGA ATATACGAAG AGGTGAACGC GAGTACGAGC 
CAGGATATGC CAGGGGAGCG AGGGGAAAAT AATTGTGGAT GGGACAAGCC TGATAATGCG 
AAGAAAATTG TCGAAGATAA CGATGAGATC CAATCGTCGG AGGAGAAGGA ATCCGCCAAG 
GTGTCCTCTT CCACGAATAT CGTCGAAAAA TCTTCCAAAT CGCACAAAAG ATCGCGCGAC 
TGCGAATGCC AAGACGTGGC CAGTAAAAAG TTCCACTTGA TCGAGGAGAT GGAAATGCAA 
GAAAATGCAA CAGGCAGCGA AAAGATGGTG GATGGAATAT CTGAAAAATG TAAACGGCAT 
TTCATAAAAG AGGCAAGGGA GTTCGTGAAG AAGGAATCGC CTCTGATAGA TCGGTGCATA 
CAGGATTTGA TAAGTAAAAA ATCGAAGGGG AATTGGGATT TGAAGAATAA TCAAGAGGAA 
TTTTTAACTT GTACAGCTTT GAACATCGCC ACCTCCGACA TATTTTCAAA CAAAAGTTGG 
GAGAATTCGG ATAAGAGAGT CGGGTCAACG TCGAACGAGC AACATCGAGC GATGGAGGCG 
AAAAAATTGT TCGACGTTCA AAATTCTGAA GATAAACCAG AAATTGGAGA TTCATCGATC 
GACAAACAAG TTGATATAAA ATCGGTCGAT CATCTTTTGA AGCAATCCTC AATTGCCGAA 
AAGCAGTCGA AAATGTGCAC GGATCTGTAC AAACAATTCT GCAAACATCT GGAAGAGATA 
GACAGTAAGA GAAAGCTTTT GATCGAGCCG GACTTCATGA AAAAAAATAA ATCGGATCAC 
GAGGAAAAGA AACGTGACGT TCTATCGCCA AGGGAGACCA AACAGTCGAA AGAGGAACAA 
ACAAAATCAT TGATCGAGGT AATTTCGGAG GACGCGACCA CGAGCGTGGA GGAACTCGAA 
GTTGACAAGT TAGTTTTCGA TTCTGATCCT GTGATGGATG CAGAGTTGAA AGATAAAATA 
TTAAAAACAA TTAATACTCC GAAAAGCGAG GAGGAGAGAG AAAAGGGAAA GAGATCGGCC 
GATAAGTTGA TGAAGATTTC CAGGGAAGCT ATGGCCAAGG GGAAATCCTT GCTCGACCCA 
TCTTTCCCTA TCTGCGGTCA GAAAAACTAT TTCAAGGACA GTAGGAGATT TTTCATGAAT 
TTGCTGGAGG ATGATTTTTC TTCCTCGGAA AAAGACGAGG ATCCAACGAA GAATATCGAC 
GAAACGGAAT CGATCATGTT CAGAGAGAAT TCAGTTGCAT TGATAGAAGA AATAGAGAAA 
TTATCGTCGA TCGAAGATAA CGAGAACTCT GTGATGAAAA TAGAAGAAAT CGAGAAATTA 
TCACCGACCG AAGGCAACAG TATTCAAGAT GGTGAAATTC TCGATATGGA AAAAGAAAAC 
GTGAACAGAG TGAGGAAAAG TTTGGAGATG CAGATTGTTC AAGGAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026300977.1
CDS	51..3101
Translation	MRGGPEEEAAERKRWIEAEQKKINDSVLALINKRKQCKPVETSEKEAEDKKRTKEDEEVATTTVVCTSDELLTLERKKKLEVSSSSDSSSASSSSGEEEDDAATQKSRIEESGGGGGTAEEGGEEASSPDRGDEILLPWKTQVPARRSVKKLVEVIEEDAKEFVAGDTMGILEERNGKIDDPPTIDREMIAGNENEIIPSCSEEEGKFKSVSVSCNILNTRSDERRREDNVEYPSKDILDNYRRKDDPHPLSGQLSSIREDMKEFCVEMDKFVERNKFVVKDSDKTKKGESSVGEEKDSCKWWDTKERKLKVKEILRQREEAERSKIVKRVEIVEVDKEEEKEEEKGEEEEEEEEASRNVKEVVKDEKNVPSCQGVYDLLNLKKCPEILLKDVKARVKTDEDDAATLKCESVKNDNSKDRLSGLYNSLFNEMNYRSGVEKNRSRKSLSSQELLTLEKIDETAEETTRSASCIDIISSDVDNSEMISVKSEPVGIKIVEASNVTALNSTDDEVSDSESVKTIINNYDKPRGIYEEVNASTSQDMPGERGENNCGWDKPDNAKKIVEDNDEIQSSEEKESAKVSSSTNIVEKSSKSHKRSRDCECQDVASKKFHLIEEMEMQENATGSEKMVDGISEKCKRHFIKEAREFVKKESPLIDRCIQDLISKKSKGNWDLKNNQEEFLTCTALNIATSDIFSNKSWENSDKRVGSTSNEQHRAMEAKKLFDVQNSEDKPEIGDSSIDKQVDIKSVDHLLKQSSIAEKQSKMCTDLYKQFCKHLEEIDSKRKLLIEPDFMKKNKSDHEEKKRDVLSPRETKQSKEEQTKSLIEVISEDATTSVEELEVDKLVFDSDPVMDAELKDKILKTINTPKSEEEREKGKRSADKLMKISREAMAKGKSLLDPSFPICGQKNYFKDSRRFFMNLLEDDFSSSEKDEDPTKNIDETESIMFRENSVALIEEIEKLSSIEDNENSVMKIEEIEKLSPTEGNSIQDGEILDMEKENVNRVRKSLEMQIVQGN

Target ORF information:

RefSeq Version	XM_026445192.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera DNA ligase 1 (LOC100578862), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026445192.1

ORF Insert Sequence:

ATGCGAGGGG GGCCCGAGGA AGAGGCGGCC GAGAGGAAAC GGTGGATCGA GGCGGAGCAG 
AAGAAAATCA ATGATAGCGT GCTCGCCCTG ATAAACAAAA GGAAGCAGTG CAAACCGGTC 
GAGACGTCCG AAAAGGAGGC GGAGGATAAG AAAAGGACGA AGGAGGACGA AGAAGTAGCC 
ACGACCACGG TTGTCTGCAC GAGCGACGAA CTGCTCACTC TGGAGAGGAA GAAAAAGTTG 
GAGGTGTCCT CATCCTCCGA CAGCTCCTCC GCGTCCTCGT CGTCGGGCGA AGAGGAGGAT 
GACGCGGCGA CGCAAAAATC GAGGATCGAG GAAAGCGGCG GCGGAGGAGG GACGGCGGAG 
GAAGGGGGGG AAGAAGCTTC TTCGCCCGAT CGAGGCGACG AAATTTTGTT ACCCTGGAAA 
ACTCAGGTTC CCGCTCGTAG GAGCGTTAAA AAGTTGGTGG AGGTGATAGA GGAGGACGCG 
AAGGAATTCG TGGCCGGCGA TACGATGGGA ATTTTGGAAG AACGAAATGG AAAGATCGAT 
GATCCACCGA CGATCGATCG CGAGATGATT GCAGGGAACG AGAACGAGAT CATTCCATCC 
TGTTCCGAGG AGGAAGGAAA GTTTAAAAGT GTCTCGGTTT CTTGCAACAT TTTGAACACG 
AGGAGCGATG AGAGGAGGAG GGAGGATAAC GTCGAATATC CGTCCAAAGA TATCCTCGAC 
AATTATCGAA GAAAGGACGA TCCGCATCCT TTGAGCGGGC AGTTGAGCAG TATCAGGGAG 
GACATGAAGG AGTTCTGCGT CGAAATGGAC AAATTCGTGG AGAGGAACAA GTTTGTGGTC 
AAAGATAGCG ATAAAACGAA AAAGGGGGAG TCGTCGGTGG GCGAGGAGAA AGATTCGTGC 
AAATGGTGGG ACACGAAGGA GAGGAAGTTG AAGGTGAAAG AGATCTTGAG GCAGAGAGAG 
GAAGCGGAGA GATCGAAAAT TGTCAAGAGG GTCGAAATTG TCGAGGTTGA TAAAGAAGAA 
GAAAAAGAAG AGGAAAAAGG AGAAGAAGAA GAAGAAGAAG AAGAAGCTTC GAGGAATGTT 
AAAGAAGTGG TTAAAGACGA GAAGAACGTT CCATCTTGCC AAGGTGTGTA CGATCTGTTG 
AATCTGAAGA AGTGTCCTGA AATTTTGTTG AAGGATGTGA AAGCTCGGGT TAAAACCGAC 
GAAGACGATG CTGCCACGTT GAAATGCGAA TCCGTTAAAA ACGACAACAG CAAGGATCGC 
CTCTCGGGAT TGTACAATTC GTTGTTCAAC GAGATGAATT ATAGAAGCGG GGTCGAGAAA 
AATCGATCGA GGAAATCGCT CAGTTCCCAA GAATTGTTGA CGTTGGAGAA AATAGACGAA 
ACAGCCGAGG AAACAACGCG GTCCGCGAGT TGTATCGACA TAATTTCCTC GGATGTTGAT 
AATTCAGAGA TGATCAGCGT TAAGAGCGAG CCTGTTGGTA TAAAAATCGT GGAAGCAAGC 
AACGTGACCG CCCTAAATTC CACCGACGAC GAGGTGTCGG ACAGCGAGTC TGTTAAAACG 
ATAATTAACA ATTACGATAA GCCGAGAGGA ATATACGAAG AGGTGAACGC GAGTACGAGC 
CAGGATATGC CAGGGGAGCG AGGGGAAAAT AATTGTGGAT GGGACAAGCC TGATAATGCG 
AAGAAAATTG TCGAAGATAA CGATGAGATC CAATCGTCGG AGGAGAAGGA ATCCGCCAAG 
GTGTCCTCTT CCACGAATAT CGTCGAAAAA TCTTCCAAAT CGCACAAAAG ATCGCGCGAC 
TGCGAATGCC AAGACGTGGC CAGTAAAAAG TTCCACTTGA TCGAGGAGAT GGAAATGCAA 
GAAAATGCAA CAGGCAGCGA AAAGATGGTG GATGGAATAT CTGAAAAATG TAAACGGCAT 
TTCATAAAAG AGGCAAGGGA GTTCGTGAAG AAGGAATCGC CTCTGATAGA TCGGTGCATA 
CAGGATTTGA TAAGTAAAAA ATCGAAGGGG AATTGGGATT TGAAGAATAA TCAAGAGGAA 
TTTTTAACTT GTACAGCTTT GAACATCGCC ACCTCCGACA TATTTTCAAA CAAAAGTTGG 
GAGAATTCGG ATAAGAGAGT CGGGTCAACG TCGAACGAGC AACATCGAGC GATGGAGGCG 
AAAAAATTGT TCGACGTTCA AAATTCTGAA GATAAACCAG AAATTGGAGA TTCATCGATC 
GACAAACAAG TTGATATAAA ATCGGTCGAT CATCTTTTGA AGCAATCCTC AATTGCCGAA 
AAGCAGTCGA AAATGTGCAC GGATCTGTAC AAACAATTCT GCAAACATCT GGAAGAGATA 
GACAGTAAGA GAAAGCTTTT GATCGAGCCG GACTTCATGA AAAAAAATAA ATCGGATCAC 
GAGGAAAAGA AACGTGACGT TCTATCGCCA AGGGAGACCA AACAGTCGAA AGAGGAACAA 
ACAAAATCAT TGATCGAGGT AATTTCGGAG GACGCGACCA CGAGCGTGGA GGAACTCGAA 
GTTGACAAGT TAGTTTTCGA TTCTGATCCT GTGATGGATG CAGAGTTGAA AGATAAAATA 
TTAAAAACAA TTAATACTCC GAAAAGCGAG GAGGAGAGAG AAAAGGGAAA GAGATCGGCC 
GATAAGTTGA TGAAGATTTC CAGGGAAGCT ATGGCCAAGG GGAAATCCTT GCTCGACCCA 
TCTTTCCCTA TCTGCGGTCA GAAAAACTAT TTCAAGGACA GTAGGAGATT TTTCATGAAT 
TTGCTGGAGG ATGATTTTTC TTCCTCGGAA AAAGACGAGG ATCCAACGAA GAATATCGAC 
GAAACGGAAT CGATCATGTT CAGAGAGAAT TCAGTTGCAT TGATAGAAGA AATAGAGAAA 
TTATCGTCGA TCGAAGATAA CGAGAACTCT GTGATGAAAA TAGAAGAAAT CGAGAAATTA 
TCACCGACCG AAGGCAACAG TATTCAAGAT GGTGAAATTC TCGATATGGA AAAAGAAAAC 
GTGAACAGAG TGAGGAAAAG TTTGGAGATG CAGATTGTTC AAGGAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100578862 ( XM_016916231.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016771720.1 Apis mellifera dynein assembly factor 1, axonemal homolog (LOC100578862), mRNA.

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CloneID	OHd20052
Clone ID Related Accession (Same CDS sequence)	XM_016916231.1
Accession Version	XM_016916231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	dynein assembly factor 1, axonemal homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007083.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGT CCTTCTTGAT TCTGAAGCCT GAGGAAGAAG AAGAATATAT CGATATCGTT 
CTCCTTGTTT CCACCGAAGA GCAGATGATA TCTGGCAAAG TTGGGCCTGG AATCGAAATG 
GTGTCCCTTG ACGGTGAGCA CTTCTTTGAT GTTCTCCAGC CCATTGACGA CCACGCAGGG 
CACGGATCCC ATCCTGAGCT TGATCACCTG GCAGTCGAAG TCTCTAACAA GATACCGAAG 
GATTCGTCGG TGGATTTCGA CGTGCTTTCC TCCTCGTCGT TGGACGTTCT CACGAAGATG 
GGCGTAGAAT TACAAAAGAA CGAGAGCTCG TCGGAGGAAC AGGCGAACAT GGCGACCGCG 
TCTACCCCGG AGCCCATACC GGAAGTCGAC AACGATGACG ACTCTAACTG GCTCTCGTAT 
TTCGACTGCA ACGCCATATT CGACGTCACC TACGAGAATC AATCATACTA CAACTCGACC 
AAAGCCGAGA AGGACAGCGA CAGCGATTTG GACGACACTT TGACATTCGA CGACGCGGAG 
GAGGGCGAGG CCCTCGTCCT CGATGAGAAC GACACGTATC CGAACTCTTA TCAACAATCG 
TTCGAGGAGA CTTGCGAGAA GAAGATGGAC GACAATAATT TCGTCGTCTC TGACAAGGTT 
TCCATACTCG AGATGAATCA AGACTCGCGC CAAGTGGCCG AGAATATCGA GTCGAACGAA 
GGGGCGAAGG AGGAGGACGA GGAGAAGGAT CGAAGTAATC TCGATTCGAG CGATCGGATC 
GACGAAAAAC TCGAAACTTC GGCGAGGATC GAGTCGGTGA CAATCGAGAA GATCGAAGAC 
GTCCCTTCGA GGGACGAGGA GGAGACGGTT GACGAGGGCA AGATAATAGA GTTGAAAACG 
GAGAGGAGAA TGGACGATTT CTCGAGCGAT TCCGAGGACG AGTACGAGGG GAATCTGATT 
TATTCCTCGC CAAACGTGAA CATATCCGCG TATTTCAAAT ACAGGGACAG TATGAAGGAG 
CCCAAGTACG AGGCGACTAT AGATCAGTTG AACGCCATGT ACTCGAAGGA ATCGAGTAAA 
TCCGTGATTT TGGAGGAAAT CGTGGAGGAG CCGTCCCTCG AGGAGGCGGG GAAAAACTCG 
GCTGCGAACG AAACGAAGGA AGACGAAGCG AAGAAGGAGG ACGAAGCGAA GAAGGAGGAC 
GAAGGGAAGG AGGCGAATTT CACGGGGACG ATTCCTTTGG ATCAAAGGAA AGGATCCAAG 
TTCGTGGAAT TGAATTCCCC CGAGGAGTAT TTGGAGAGAT TGGCGGAGAT CACGGAGTCG 
GATTGTCCGA GAACCGAGGA GGAGGCTCAC GAGAAGTTGA AGAGGATCGC CGAGGGGAAG 
GCGGAGATAG AGAACAGGAA GAACGAGGCT CTGGAGGATC TGTCGGTGGA GTTCGATAAG 
ATCGAGAAAC TCGTCGCGGA GACCAAGTCG TCCGCGGACC AAGAATACGA CAGCGGATCA 
GACGAGAGCT CGGACGAGAC GAGGGAGGAT ATCGATATAG GCAAGGATAT AGAGCTGCCT 
TTGACCAAGG ACCAAGTAGC GGAGAGCTTT AAATTCAAAA CTATTCAAAA GAGCGTGGAG 
GAGGAGGAGA AGCGTCGCGC GGAATTGTTG CAAGAATGCC TCCAAGTGAT TCCAAAAATA 
CCAGAAACGG AGGAGGATCT CGTGGACGAG CGAGATATCC AAGATACGAT CGCGAAAGAG 
AAGGTGGACG ACGATATCCT GTCGGAGGAG GCCTCGAAAG AGGAGGAGGA GGAGAAGGAG 
GAGAAGGAGA AGGAAGAGGA GGAGGTGAAG GAGGAGGAAG GCGAGAGCGC GAAGACGATC 
GTCGACGAGG CCTCGACGAC GGAAAGCAAG TCAACGGTGC AAGGCATCGT CCAAGACATT 
ATCGACGAGA CCGAGGATTC GTTGTTTTGG CGATTCAATA AGGAGCCGGA GAGGACTTAC 
ATCAAGGGGA AGGTGTACGA TTTCGACGAG AAGAAACACG GCGCCAGAAT GACCGAGGAG 
TTTTTGAAAA AGCACTGCAA GCAGCATAAA CTTTATCAAA CACCGCATCT GAACGACGTA 
CTTTATCTTC ATTACAAAGG TTTCTCTTTC ATCGAGAACC TCGAGAAGTA TACAGGCTTG 
AAGTGTTTGT GGCTGGAGAA CAACGGGATC AGGGAGATCG CGAATCTGGA GAATCAGGGC 
GAGCTCAAGT GCTTGTTCCT TCACAATAAT TTGATCAGCA GGATCGAGAA TCTCGAGTAC 
CTGACCAAGC TCGACACTCT GAACCTCTCT TACAACACTA TACGAAGGAT TGAGAATCTC 
GATAGCCTCA AGTTCCTGAA TACTCTAAAC CTGTCGCACA ACTACTTGCA AAGTACCGGT 
GACATTGAGC ATCTCCGATT GCTGGACAGC CTCTCGGTAC TGGATATTTC TCACAACAGG 
ATAGACACCG ACGAAGTCGT TAATATATTG GGAGACATGA AAGAGTTGCG CGTCGTGTCC 
CTGATGGGAA ATCCGATCCT GAAGGCGATC AAACTGTATC GCAAGACAAT GATCCTCAAG 
TGTAAGAATC TCAAGTATCT AGACGATAGA CCGGTATTTC CAAGAGATCG TGCCTGCGCC 
GAGGCTTGGA TGCGAGGGGG GCCCGAGGAA GAGGCGGCCG AGAGGAAACG GTGGATCGAG 
GCGGAGCAGA AGAAAATCAA TGATAGCGTG CTCGCCCTGA TAAACAAAAG GAAGCAGTGC 
AAACCGGTCG AGACGTCCGA AAAGGAGGCG GAGGATAAGA AAAGGACGAA GGAGGACGAA 
GAAGTAGCCA CGACCACGGT TGTCTGCACG AGCGACGAAC TGCTCACTCT GGAGAGGAAG 
AAAAAGTTGG AGGTGTCCTC ATCCTCCGAC AGCTCCTCCG CGTCCTCGTC GTCGGGCGAA 
GAGGAGGATG ACGCGGCGAC GCAAAAATCG AGGATCGAGG AAAGCGGCGG CGGAGGAGGG 
ACGGCGGAGG AAGGGGGGGA AGAAGCTTCT TCGCCCGATC GAGGCGACGA AATTTTGTTA 
CCCTGGAAAA CTCAGGTTCC CGCTCGTAGG AGCGTTAAAA AGTTGGTGGA GGTGATAGAG 
GAGGACGCGA AGGAATTCGT GGCCGGCGAT ACGATGGGAA TTTTGGAAGA ACGAAATGGA 
AAGATCGATG ATCCACCGAC GATCGATCGC GAGATGATTG CAGGGAACGA GAACGAGATC 
ATTCCATCCT GTTCCGAGGA GGAAGGAAAG TTTAAAAGTG TCTCGGTTTC TTGCAACATT 
TTGAACACGA GGAGCGATGA GAGGAGGAGG GAGGATAACG TCGAATATCC GTCCAAAGAT 
ATCCTCGACA ATTATCGAAG AAAGGACGAT CCGCATCCTT TGAGCGGGCA GTTGAGCAGT 
ATCAGGGAGG ACATGAAGGA GTTCTGCGTC GAAATGGACA AATTCGTGGA GAGGAACAAG 
TTTGTGGTCA AAGATAGCGA TAAAACGAAA AAGGGGGAGT CGTCGGTGGG CGAGGAGAAA 
GATTCGTGCA AATGGTGGGA CACGAAGGAG AGGAAGTTGA AGGTGAAAGA GATCTTGAGG 
CAGAGAGAGG AAGCGGAGAG ATCGAAAATT GTCAAGAGGG TCGAAATTGT CGAGGTTGAT 
AAAGAAGAAG AAAAAGAAGA GGAAAAAGGA GAAGAAGAAG AAGAAGAAGA AGAAGCTTCG 
AGGAATGTTA AAGAAGTGGT TAAAGACGAG AAGAACGTTC CATCTTGCCA AGGTGTGTAC 
GATCTGTTGA ATCTGAAGAA GTGTCCTGAA ATTTTGTTGA AGGATGTGAA AGCTCGGGTT 
AAAACCGACG AAGACGATGC TGCCACGTTG AAATGCGAAT CCGTTAAAAA CGACAACAGC 
AAGGATCGCC TCTCGGGATT GTACAATTCG TTGTTCAACG AGATGAATTA TAGAAGCGGG 
GTCGAGAAAA ATCGATCGAG GAAATCGCTC AGTTCCCAAG AATTGTTGAC GTTGGAGAAA 
ATAGACGAAA CAGCCGAGGA AACAACGCGG TCCGCGAGTT GTATCGACAT AATTTCCTCG 
GATGTTGATA ATTCAGAGAT GATCAGCGTT AAGAGCGAGC CTGTTGGTAT AAAAATCGTG 
GAAGCAAGCA ACGTGACCGC CCTAAATTCC ACCGACGACG AGGTGTCGGA CAGCGAGTCT 
GTTAAAACGA TAATTAACAA TTACGATAAG CCGAGAGGAA TATACGAAGA GGTGAACGCG 
AGTACGAGCC AGGATATGCC AGGGGAGCGA GGGGAAAATA ATTGTGGATG GGACAAGCCT 
GATAATGCGA AGAAAATTGT CGAAGATAAC GATGAGATCC AATCGTCGGA GGAGAAGGAA 
TCCGCCAAGG TGTCCTCTTC CACGAATATC GTCGAAAAAT CTTCCAAATC GCACAAAAGA 
TCGCGCGACT GCGAATGCCA AGACGTGGCC AGTAAAAAGT TCCACTTGAT CGAGGAGATG 
GAAATGCAAG AAAATGCAAC AGGCAGCGAA AAGATGGTGG ATGGAATATC TGAAAAATGT 
AAACGGCATT TCATAAAAGA GGCAAGGGAG TTCGTGAAGA AGGAATCGCC TCTGATAGAT 
CGGTGCATAG AGGATTTGAT AAGTAAAAAA TCGAAGGGGA ATTGGGATTT GAAGAATAAT 
CAAGAGGAAT TTTTAACTTG TACAGCTTTG AACATCGCCA CCTCCGACAT ATTTTCAAAC 
AAAAGTTGGG AGAATTCGGA TAAGAGGGTC AGGTCAACGT CGAACGAGCA ACATCGAGCG 
ATGGAGGCGA AAAAATTGTT CGACGTTCAA AATTCTGAAG ATAAACGAGA AGTTGGAGAT 
TCATCGATCG ACAAACAAGT CGATATAAAA TCGGTCGATC ATCTTTTGAA GCAATCCTCA 
ATTGCCGAAA AGCAGTCGAA AATGTGCACG GATCTGTACA AACAATTCTG CAAACATCTG 
GAAGAGATAG ACAGTAAGAG AAAGCTTTTG ATCGAGCCGG ACTTCATGAA AAAAAATAAA 
TCGGATCACG AGGAAAAGAA ACGTGACGTT CTATCGCCAA GGGAGACCAA ACAGTCGAAA 
GAGGAACAAA CAAAATCATT GATCGAGGTA ATTTCGGAGG ACGCGACCAC GAGCGTGGAG 
GAACTCGAAG TTGACAAGTT AGTTTTCGAT TCTGATCCTG TGATGGATGC AGAGTTGAAA 
GATAAAATAT TAAAAACAAT TAATACTCCG AAAAGCGAGG AGGAGAGAGA AAAGGGAAAG 
AGATCGGCCG ATAAGTTGAT GAAGATTTCC AGGGAAGCTA TGGCCAAGGG GAAATCCTTG 
CTCGACCCAT CTTTCCCTAT CTGCGGTCAG AAAAACTATT TCAAGGACAG TAGGAGATTT 
TTCATGAATT TGCTGGAGGA TGATTTTTCT TCCTCGGAAA AAGACGAGGA TCCAACGAAG 
AATATCGACG AAACGGAATC GATCATGTTC AGAGAGAATT CAGTTGCATT GATAGAAGAA 
ATAGAGAAAT TATCGTCGAT CGAAGATAAC GAGAACTCTG TGATGAAAAT AGAAGAAATC 
GAGAAATTAT CACCGACCGA AGGCAACAGT ATTCAAGATG GTGAAATTCT CGATATGGAA 
AAAGAAAACG TGAACAGAGT GAGGAAAAGT TTGGAGATGC AGATTGTTCA AGGAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016771720.1
CDS	43..5742
Translation	MNVSFLILKPEEEEEYIDIVLLVSTEEQMISGKVGPGIEMVSLDGEHFFDVLQPIDDHAGHGSHPELDHLAVEVSNKIPKDSSVDFDVLSSSSLDVLTKMGVELQKNESSSEEQANMATASTPEPIPEVDNDDDSNWLSYFDCNAIFDVTYENQSYYNSTKAEKDSDSDLDDTLTFDDAEEGEALVLDENDTYPNSYQQSFEETCEKKMDDNNFVVSDKVSILEMNQDSRQVAENIESNEGAKEEDEEKDRSNLDSSDRIDEKLETSARIESVTIEKIEDVPSRDEEETVDEGKIIELKTERRMDDFSSDSEDEYEGNLIYSSPNVNISAYFKYRDSMKEPKYEATIDQLNAMYSKESSKSVILEEIVEEPSLEEAGKNSAANETKEDEAKKEDEAKKEDEGKEANFTGTIPLDQRKGSKFVELNSPEEYLERLAEITESDCPRTEEEAHEKLKRIAEGKAEIENRKNEALEDLSVEFDKIEKLVAETKSSADQEYDSGSDESSDETREDIDIGKDIELPLTKDQVAESFKFKTIQKSVEEEEKRRAELLQECLQVIPKIPETEEDLVDERDIQDTIAKEKVDDDILSEEASKEEEEEKEEKEKEEEEVKEEEGESAKTIVDEASTTESKSTVQGIVQDIIDETEDSLFWRFNKEPERTYIKGKVYDFDEKKHGARMTEEFLKKHCKQHKLYQTPHLNDVLYLHYKGFSFIENLEKYTGLKCLWLENNGIREIANLENQGELKCLFLHNNLISRIENLEYLTKLDTLNLSYNTIRRIENLDSLKFLNTLNLSHNYLQSTGDIEHLRLLDSLSVLDISHNRIDTDEVVNILGDMKELRVVSLMGNPILKAIKLYRKTMILKCKNLKYLDDRPVFPRDRACAEAWMRGGPEEEAAERKRWIEAEQKKINDSVLALINKRKQCKPVETSEKEAEDKKRTKEDEEVATTTVVCTSDELLTLERKKKLEVSSSSDSSSASSSSGEEEDDAATQKSRIEESGGGGGTAEEGGEEASSPDRGDEILLPWKTQVPARRSVKKLVEVIEEDAKEFVAGDTMGILEERNGKIDDPPTIDREMIAGNENEIIPSCSEEEGKFKSVSVSCNILNTRSDERRREDNVEYPSKDILDNYRRKDDPHPLSGQLSSIREDMKEFCVEMDKFVERNKFVVKDSDKTKKGESSVGEEKDSCKWWDTKERKLKVKEILRQREEAERSKIVKRVEIVEVDKEEEKEEEKGEEEEEEEEASRNVKEVVKDEKNVPSCQGVYDLLNLKKCPEILLKDVKARVKTDEDDAATLKCESVKNDNSKDRLSGLYNSLFNEMNYRSGVEKNRSRKSLSSQELLTLEKIDETAEETTRSASCIDIISSDVDNSEMISVKSEPVGIKIVEASNVTALNSTDDEVSDSESVKTIINNYDKPRGIYEEVNASTSQDMPGERGENNCGWDKPDNAKKIVEDNDEIQSSEEKESAKVSSSTNIVEKSSKSHKRSRDCECQDVASKKFHLIEEMEMQENATGSEKMVDGISEKCKRHFIKEAREFVKKESPLIDRCIEDLISKKSKGNWDLKNNQEEFLTCTALNIATSDIFSNKSWENSDKRVRSTSNEQHRAMEAKKLFDVQNSEDKREVGDSSIDKQVDIKSVDHLLKQSSIAEKQSKMCTDLYKQFCKHLEEIDSKRKLLIEPDFMKKNKSDHEEKKRDVLSPRETKQSKEEQTKSLIEVISEDATTSVEELEVDKLVFDSDPVMDAELKDKILKTINTPKSEEEREKGKRSADKLMKISREAMAKGKSLLDPSFPICGQKNYFKDSRRFFMNLLEDDFSSSEKDEDPTKNIDETESIMFRENSVALIEEIEKLSSIEDNENSVMKIEEIEKLSPTEGNSIQDGEILDMEKENVNRVRKSLEMQIVQGN

Target ORF information:

RefSeq Version	XM_016916231.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera dynein assembly factor 1, axonemal homolog (LOC100578862), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016916231.1

ORF Insert Sequence:

ATGAACGTGT CCTTCTTGAT TCTGAAGCCT GAGGAAGAAG AAGAATATAT CGATATCGTT 
CTCCTTGTTT CCACCGAAGA GCAGATGATA TCTGGCAAAG TTGGGCCTGG AATCGAAATG 
GTGTCCCTTG ACGGTGAGCA CTTCTTTGAT GTTCTCCAGC CCATTGACGA CCACGCAGGG 
CACGGATCCC ATCCTGAGCT TGATCACCTG GCAGTCGAAG TCTCTAACAA GATACCGAAG 
GATTCGTCGG TGGATTTCGA CGTGCTTTCC TCCTCGTCGT TGGACGTTCT CACGAAGATG 
GGCGTAGAAT TACAAAAGAA CGAGAGCTCG TCGGAGGAAC AGGCGAACAT GGCGACCGCG 
TCTACCCCGG AGCCCATACC GGAAGTCGAC AACGATGACG ACTCTAACTG GCTCTCGTAT 
TTCGACTGCA ACGCCATATT CGACGTCACC TACGAGAATC AATCATACTA CAACTCGACC 
AAAGCCGAGA AGGACAGCGA CAGCGATTTG GACGACACTT TGACATTCGA CGACGCGGAG 
GAGGGCGAGG CCCTCGTCCT CGATGAGAAC GACACGTATC CGAACTCTTA TCAACAATCG 
TTCGAGGAGA CTTGCGAGAA GAAGATGGAC GACAATAATT TCGTCGTCTC TGACAAGGTT 
TCCATACTCG AGATGAATCA AGACTCGCGC CAAGTGGCCG AGAATATCGA GTCGAACGAA 
GGGGCGAAGG AGGAGGACGA GGAGAAGGAT CGAAGTAATC TCGATTCGAG CGATCGGATC 
GACGAAAAAC TCGAAACTTC GGCGAGGATC GAGTCGGTGA CAATCGAGAA GATCGAAGAC 
GTCCCTTCGA GGGACGAGGA GGAGACGGTT GACGAGGGCA AGATAATAGA GTTGAAAACG 
GAGAGGAGAA TGGACGATTT CTCGAGCGAT TCCGAGGACG AGTACGAGGG GAATCTGATT 
TATTCCTCGC CAAACGTGAA CATATCCGCG TATTTCAAAT ACAGGGACAG TATGAAGGAG 
CCCAAGTACG AGGCGACTAT AGATCAGTTG AACGCCATGT ACTCGAAGGA ATCGAGTAAA 
TCCGTGATTT TGGAGGAAAT CGTGGAGGAG CCGTCCCTCG AGGAGGCGGG GAAAAACTCG 
GCTGCGAACG AAACGAAGGA AGACGAAGCG AAGAAGGAGG ACGAAGCGAA GAAGGAGGAC 
GAAGGGAAGG AGGCGAATTT CACGGGGACG ATTCCTTTGG ATCAAAGGAA AGGATCCAAG 
TTCGTGGAAT TGAATTCCCC CGAGGAGTAT TTGGAGAGAT TGGCGGAGAT CACGGAGTCG 
GATTGTCCGA GAACCGAGGA GGAGGCTCAC GAGAAGTTGA AGAGGATCGC CGAGGGGAAG 
GCGGAGATAG AGAACAGGAA GAACGAGGCT CTGGAGGATC TGTCGGTGGA GTTCGATAAG 
ATCGAGAAAC TCGTCGCGGA GACCAAGTCG TCCGCGGACC AAGAATACGA CAGCGGATCA 
GACGAGAGCT CGGACGAGAC GAGGGAGGAT ATCGATATAG GCAAGGATAT AGAGCTGCCT 
TTGACCAAGG ACCAAGTAGC GGAGAGCTTT AAATTCAAAA CTATTCAAAA GAGCGTGGAG 
GAGGAGGAGA AGCGTCGCGC GGAATTGTTG CAAGAATGCC TCCAAGTGAT TCCAAAAATA 
CCAGAAACGG AGGAGGATCT CGTGGACGAG CGAGATATCC AAGATACGAT CGCGAAAGAG 
AAGGTGGACG ACGATATCCT GTCGGAGGAG GCCTCGAAAG AGGAGGAGGA GGAGAAGGAG 
GAGAAGGAGA AGGAAGAGGA GGAGGTGAAG GAGGAGGAAG GCGAGAGCGC GAAGACGATC 
GTCGACGAGG CCTCGACGAC GGAAAGCAAG TCAACGGTGC AAGGCATCGT CCAAGACATT 
ATCGACGAGA CCGAGGATTC GTTGTTTTGG CGATTCAATA AGGAGCCGGA GAGGACTTAC 
ATCAAGGGGA AGGTGTACGA TTTCGACGAG AAGAAACACG GCGCCAGAAT GACCGAGGAG 
TTTTTGAAAA AGCACTGCAA GCAGCATAAA CTTTATCAAA CACCGCATCT GAACGACGTA 
CTTTATCTTC ATTACAAAGG TTTCTCTTTC ATCGAGAACC TCGAGAAGTA TACAGGCTTG 
AAGTGTTTGT GGCTGGAGAA CAACGGGATC AGGGAGATCG CGAATCTGGA GAATCAGGGC 
GAGCTCAAGT GCTTGTTCCT TCACAATAAT TTGATCAGCA GGATCGAGAA TCTCGAGTAC 
CTGACCAAGC TCGACACTCT GAACCTCTCT TACAACACTA TACGAAGGAT TGAGAATCTC 
GATAGCCTCA AGTTCCTGAA TACTCTAAAC CTGTCGCACA ACTACTTGCA AAGTACCGGT 
GACATTGAGC ATCTCCGATT GCTGGACAGC CTCTCGGTAC TGGATATTTC TCACAACAGG 
ATAGACACCG ACGAAGTCGT TAATATATTG GGAGACATGA AAGAGTTGCG CGTCGTGTCC 
CTGATGGGAA ATCCGATCCT GAAGGCGATC AAACTGTATC GCAAGACAAT GATCCTCAAG 
TGTAAGAATC TCAAGTATCT AGACGATAGA CCGGTATTTC CAAGAGATCG TGCCTGCGCC 
GAGGCTTGGA TGCGAGGGGG GCCCGAGGAA GAGGCGGCCG AGAGGAAACG GTGGATCGAG 
GCGGAGCAGA AGAAAATCAA TGATAGCGTG CTCGCCCTGA TAAACAAAAG GAAGCAGTGC 
AAACCGGTCG AGACGTCCGA AAAGGAGGCG GAGGATAAGA AAAGGACGAA GGAGGACGAA 
GAAGTAGCCA CGACCACGGT TGTCTGCACG AGCGACGAAC TGCTCACTCT GGAGAGGAAG 
AAAAAGTTGG AGGTGTCCTC ATCCTCCGAC AGCTCCTCCG CGTCCTCGTC GTCGGGCGAA 
GAGGAGGATG ACGCGGCGAC GCAAAAATCG AGGATCGAGG AAAGCGGCGG CGGAGGAGGG 
ACGGCGGAGG AAGGGGGGGA AGAAGCTTCT TCGCCCGATC GAGGCGACGA AATTTTGTTA 
CCCTGGAAAA CTCAGGTTCC CGCTCGTAGG AGCGTTAAAA AGTTGGTGGA GGTGATAGAG 
GAGGACGCGA AGGAATTCGT GGCCGGCGAT ACGATGGGAA TTTTGGAAGA ACGAAATGGA 
AAGATCGATG ATCCACCGAC GATCGATCGC GAGATGATTG CAGGGAACGA GAACGAGATC 
ATTCCATCCT GTTCCGAGGA GGAAGGAAAG TTTAAAAGTG TCTCGGTTTC TTGCAACATT 
TTGAACACGA GGAGCGATGA GAGGAGGAGG GAGGATAACG TCGAATATCC GTCCAAAGAT 
ATCCTCGACA ATTATCGAAG AAAGGACGAT CCGCATCCTT TGAGCGGGCA GTTGAGCAGT 
ATCAGGGAGG ACATGAAGGA GTTCTGCGTC GAAATGGACA AATTCGTGGA GAGGAACAAG 
TTTGTGGTCA AAGATAGCGA TAAAACGAAA AAGGGGGAGT CGTCGGTGGG CGAGGAGAAA 
GATTCGTGCA AATGGTGGGA CACGAAGGAG AGGAAGTTGA AGGTGAAAGA GATCTTGAGG 
CAGAGAGAGG AAGCGGAGAG ATCGAAAATT GTCAAGAGGG TCGAAATTGT CGAGGTTGAT 
AAAGAAGAAG AAAAAGAAGA GGAAAAAGGA GAAGAAGAAG AAGAAGAAGA AGAAGCTTCG 
AGGAATGTTA AAGAAGTGGT TAAAGACGAG AAGAACGTTC CATCTTGCCA AGGTGTGTAC 
GATCTGTTGA ATCTGAAGAA GTGTCCTGAA ATTTTGTTGA AGGATGTGAA AGCTCGGGTT 
AAAACCGACG AAGACGATGC TGCCACGTTG AAATGCGAAT CCGTTAAAAA CGACAACAGC 
AAGGATCGCC TCTCGGGATT GTACAATTCG TTGTTCAACG AGATGAATTA TAGAAGCGGG 
GTCGAGAAAA ATCGATCGAG GAAATCGCTC AGTTCCCAAG AATTGTTGAC GTTGGAGAAA 
ATAGACGAAA CAGCCGAGGA AACAACGCGG TCCGCGAGTT GTATCGACAT AATTTCCTCG 
GATGTTGATA ATTCAGAGAT GATCAGCGTT AAGAGCGAGC CTGTTGGTAT AAAAATCGTG 
GAAGCAAGCA ACGTGACCGC CCTAAATTCC ACCGACGACG AGGTGTCGGA CAGCGAGTCT 
GTTAAAACGA TAATTAACAA TTACGATAAG CCGAGAGGAA TATACGAAGA GGTGAACGCG 
AGTACGAGCC AGGATATGCC AGGGGAGCGA GGGGAAAATA ATTGTGGATG GGACAAGCCT 
GATAATGCGA AGAAAATTGT CGAAGATAAC GATGAGATCC AATCGTCGGA GGAGAAGGAA 
TCCGCCAAGG TGTCCTCTTC CACGAATATC GTCGAAAAAT CTTCCAAATC GCACAAAAGA 
TCGCGCGACT GCGAATGCCA AGACGTGGCC AGTAAAAAGT TCCACTTGAT CGAGGAGATG 
GAAATGCAAG AAAATGCAAC AGGCAGCGAA AAGATGGTGG ATGGAATATC TGAAAAATGT 
AAACGGCATT TCATAAAAGA GGCAAGGGAG TTCGTGAAGA AGGAATCGCC TCTGATAGAT 
CGGTGCATAG AGGATTTGAT AAGTAAAAAA TCGAAGGGGA ATTGGGATTT GAAGAATAAT 
CAAGAGGAAT TTTTAACTTG TACAGCTTTG AACATCGCCA CCTCCGACAT ATTTTCAAAC 
AAAAGTTGGG AGAATTCGGA TAAGAGGGTC AGGTCAACGT CGAACGAGCA ACATCGAGCG 
ATGGAGGCGA AAAAATTGTT CGACGTTCAA AATTCTGAAG ATAAACGAGA AGTTGGAGAT 
TCATCGATCG ACAAACAAGT CGATATAAAA TCGGTCGATC ATCTTTTGAA GCAATCCTCA 
ATTGCCGAAA AGCAGTCGAA AATGTGCACG GATCTGTACA AACAATTCTG CAAACATCTG 
GAAGAGATAG ACAGTAAGAG AAAGCTTTTG ATCGAGCCGG ACTTCATGAA AAAAAATAAA 
TCGGATCACG AGGAAAAGAA ACGTGACGTT CTATCGCCAA GGGAGACCAA ACAGTCGAAA 
GAGGAACAAA CAAAATCATT GATCGAGGTA ATTTCGGAGG ACGCGACCAC GAGCGTGGAG 
GAACTCGAAG TTGACAAGTT AGTTTTCGAT TCTGATCCTG TGATGGATGC AGAGTTGAAA 
GATAAAATAT TAAAAACAAT TAATACTCCG AAAAGCGAGG AGGAGAGAGA AAAGGGAAAG 
AGATCGGCCG ATAAGTTGAT GAAGATTTCC AGGGAAGCTA TGGCCAAGGG GAAATCCTTG 
CTCGACCCAT CTTTCCCTAT CTGCGGTCAG AAAAACTATT TCAAGGACAG TAGGAGATTT 
TTCATGAATT TGCTGGAGGA TGATTTTTCT TCCTCGGAAA AAGACGAGGA TCCAACGAAG 
AATATCGACG AAACGGAATC GATCATGTTC AGAGAGAATT CAGTTGCATT GATAGAAGAA 
ATAGAGAAAT TATCGTCGAT CGAAGATAAC GAGAACTCTG TGATGAAAAT AGAAGAAATC 
GAGAAATTAT CACCGACCGA AGGCAACAGT ATTCAAGATG GTGAAATTCT CGATATGGAA 
AAAGAAAACG TGAACAGAGT GAGGAAAAGT TTGGAGATGC AGATTGTTCA AGGAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100578862 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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