RCN1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	RCN1
Entrez Gene ID	839135
Full Name	ARM repeat superfamily protein
Synonyms	ATB BETA BETA,EER1,ENHANCED ETHYLENE RESPONSE 1,F2J7.19,F2J7_19,PHOSPHOPROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT A,REGA,ROOTS CURL IN NPA,SERINE/THREONINE PROTEIN PHOSPHATASE TYPE 2A REGULATORY SUBUNIT A
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_102360.4	NP_173920.1	ARM repeat superfamily protein

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ACR279C	NP_983681.1
Neurospora crassa	NCU00488	XP_001728513.1
Bos taurus (cattle)	PPP2R1A	NP_001032554.1
Mus musculus (house mouse)	Ppp2r1a	NP_058587.1
Drosophila melanogaster (fruit fly)	Pp2A-29B	NP_001027225.1
Arabidopsis thaliana (thale cress)	RCN1	NP_173920.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009105	XP_319856.4
Kluyveromyces lactis	KLLA0F07689g	XP_455428.1
Schizosaccharomyces pombe (fission yeast)	paa1	NP_594948.1
Homo sapiens (human)	PPP2R1A	NP_055040.2
Pan troglodytes (chimpanzee)	PPP2R1A	XP_001174546.1
	PPP2R1A	XP_001116604.1
Canis lupus familiaris (dog)	LOC484335	XP_850993.1
Rattus norvegicus (Norway rat)	Ppp2r1a	NP_476481.1
Caenorhabditis elegans (roundworm)	paa-1	NP_498162.2
Magnaporthe oryzae (rice blast fungus)	MGG_05671	XP_003710570.1
Xenopus tropicalis (tropical clawed frog)	ppp2r1a	NP_989405.1
Saccharomyces cerevisiae (baker's yeast)	TPD3	NP_009386.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Protein phosphorylation plays a key role in ethylene signaling, which is mediated by the activities of ethylene receptors, CTR1, and RCN1.
Title: Stable isotope metabolic labeling-based quantitative phosphoproteomic analysis of Arabidopsis mutants reveals ethylene-regulated time-dependent phosphoproteins and putative substrates of constitutive triple response 1 kinase.

RCN1 controls signaling between methyl jasmonate and abscisic acid. MeJA and ABA failed to induce stomatal closure in RCN1 mutants.
Title: Roles of RCN1, regulatory A subunit of protein phosphatase 2A, in methyl jasmonate signaling and signal crosstalk between methyl jasmonate and abscisic acid.

This study showed that hypocotyl and root growth exhibit different requirements for RCN1-mediated regulation of PP2A activity.
Title: Specificity of RCN1-mediated protein phosphatase 2A regulation in meristem organization and stress response in roots.

The RCN1 protein affects overall hypocotyl elongation via negative regulation of ethylene synthesis in etiolated seedlings.
Title: RCN1-regulated phosphatase activity and EIN2 modulate hypocotyl gravitropism by a mechanism that does not require ethylene signaling.

The following RCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb24467	NM_102360.4 Latest version!	Arabidopsis thaliana ARM repeat superfamily protein (RCN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RCN1 ( NM_102360.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_173920.1 Arabidopsis thaliana ARM repeat superfamily protein (RCN1), mRNA.

Price & Availability↑

CloneID	OAb24467
Clone ID Related Accession (Same CDS sequence)	NM_102360.4
Accession Version	NM_102360.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ARM repeat superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_102360.3.

ATGGCTATGG TAGATGAACC GTTGTATCCC ATTGCTGTGC TTATAGATGA GCTTAAGAAT 
GATGATATTC AGCTTCGTTT GAACTCGATC CGTCGCTTAT CTACTATAGC TCGTGCTCTT 
GGAGAGGAGC GTACAAGGAA GGAGTTAATC CCTTTTTTGA GTGAGAATAG TGACGATGAC 
GATGAGGTGC TTCTTGCAAT GGCTGAGGAG TTAGGAGTTT TTATTCCGTT TGTTGGAGGA 
ATTGAGTTTG CGCATGTTCT TCTTCCTCCT TTGGAATCTC TATGTACTGT TGAAGAGACT 
TGTGTGAGAG AAAAAGCTGT GGAATCGCTT TGTAAGATTG GATCTCAGAT GAAAGAGAAT 
GATCTTGTTG AATCTTTTGT TCCTCTTGTG AAGAGGTTAG CGGGTGGTGA ATGGTTTGCA 
GCAAGAGTTT CTGCATGTGG TATATTTCAT GTTGCATACC AAGGTTGCAC TGATGTGTTG 
AAGACTGAGT TACGGGCTAC TTATAGCCAG TTGTGCAAAG ATGATATGCC AATGGTGCGA 
AGAGCTGCTG CATCTAACCT GGGGAAATTT GCTACAACTG TCGAGTCTAC CTTTTTGATT 
GCTGAGATCA TGACTATGTT CGATGATCTT ACTAAAGATG ACCAAGATTC TGTGAGACTA 
TTGGCTGTTG AAGGGTGTGC AGCTCTTGGA AAGTTGTTGG AACCTCAGGA TTGTGTTGCA 
CGCATTTTAC CTGTTATTGT TAATTTCTCT CAGGATAAAT CTTGGAGGGT GCGCTACATG 
GTTGCTAATC AGCTATATGA ACTTTGTGAG GCAGTGGGTC CTGATTGCAC GAGGACGGAT 
TTGGTTCCAG CATATGTGAG ATTGCTAAGG GACAATGAGG CTGAAGTGCG AATAGCAGCA 
GCGGGAAAAG TGACCAAGTT CTGTCGGCTT TTGAATCCAG AGCTTGCGAT TCAGCACATC 
CTTCCTTGTG TGAAGGAATT ATCATCGGAT TCTTCTCAAC ATGTCCGCTC TGCTCTAGCT 
TCAGTAATAA TGGGGATGGC TCCTATCCTT GGGAAGGACT CAACCATTGA GCATCTGTTA 
CCAATTTTTC TTTCCCTTTT GAAAGATGAA TTTCCTGATG TGCGCCTTAA CATCATAAGC 
AAGTTAGATC AAGTCAACCA GGTTATTGGA ATTGATCTAC TATCTCAATC CTTGTTACCG 
GCCATTGTAG AACTTGCTGA GGATCGGCAC TGGAGAGTCC GACTTGCAAT AATAGAGTAT 
GTTCCACTGT TGGCCAGCCA GTTAGGTATA GGATTTTTCG ATGACAAGCT CGGAGCCCTT 
TGCATGCAAT GGCTGCAAGA CAAGGTCTAC TCTATCCGCG AAGCTGCAGC AAACAACCTA 
AAGCGCCTCG CAGAGGAGTT TGGTCCTGAA TGGGCAATGC AGCACTTAGT TCCCCAGGTA 
TTGGACATGG TCAACAATCC GCACTACCTA CACAGGATGA TGGTTCTACG CGCAATATCT 
CTCATGGCGC CTGTAATGGG ATCAGAAATC ACATGCTCTA AGTTTCTTCC TGTGGTTGTT 
GAAGCATCAA AAGACAGAGT TCCAAACATC AAGTTCAACG TTGCCAAACT TCTGCAATCC 
CTCATCCCCA TAGTCGACCA ATCAGTGGTG GACAAAACAA TCCGTCAGTG TTTGGTGGAC 
CTGAGCGAAG ACCCTGATGT TGATGTTCGT TATTTTGCAA ATCAAGCACT TAATTCCATC 
GATGGTTCCA CAGCAGCACA ATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_173920.1
CDS	176..1942
Translation	MAMVDEPLYPIAVLIDELKNDDIQLRLNSIRRLSTIARALGEERTRKELIPFLSENSDDDDEVLLAMAEELGVFIPFVGGIEFAHVLLPPLESLCTVEETCVREKAVESLCKIGSQMKENDLVESFVPLVKRLAGGEWFAARVSACGIFHVAYQGCTDVLKTELRATYSQLCKDDMPMVRRAAASNLGKFATTVESTFLIAEIMTMFDDLTKDDQDSVRLLAVEGCAALGKLLEPQDCVARILPVIVNFSQDKSWRVRYMVANQLYELCEAVGPDCTRTDLVPAYVRLLRDNEAEVRIAAAGKVTKFCRLLNPELAIQHILPCVKELSSDSSQHVRSALASVIMGMAPILGKDSTIEHLLPIFLSLLKDEFPDVRLNIISKLDQVNQVIGIDLLSQSLLPAIVELAEDRHWRVRLAIIEYVPLLASQLGIGFFDDKLGALCMQWLQDKVYSIREAAANNLKRLAEEFGPEWAMQHLVPQVLDMVNNPHYLHRMMVLRAISLMAPVMGSEITCSKFLPVVVEASKDRVPNIKFNVAKLLQSLIPIVDQSVVDKTIRQCLVDLSEDPDVDVRYFANQALNSIDGSTAAQS

Target ORF information:

RefSeq Version	NM_102360.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ARM repeat superfamily protein (RCN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_102360.4

ORF Insert Sequence:

ATGGCTATGG TAGATGAACC GTTGTATCCC ATTGCTGTGC TTATAGATGA GCTTAAGAAT 
GATGATATTC AGCTTCGTTT GAACTCGATC CGTCGCTTAT CTACTATAGC TCGTGCTCTT 
GGAGAGGAGC GTACAAGGAA GGAGTTAATC CCTTTTTTGA GTGAGAATAG TGACGATGAC 
GATGAGGTGC TTCTTGCAAT GGCTGAGGAG TTAGGAGTTT TTATTCCGTT TGTTGGAGGA 
ATTGAGTTTG CGCATGTTCT TCTTCCTCCT TTGGAATCTC TATGTACTGT TGAAGAGACT 
TGTGTGAGAG AAAAAGCTGT GGAATCGCTT TGTAAGATTG GATCTCAGAT GAAAGAGAAT 
GATCTTGTTG AATCTTTTGT TCCTCTTGTG AAGAGGTTAG CGGGTGGTGA ATGGTTTGCA 
GCAAGAGTTT CTGCATGTGG TATATTTCAT GTTGCATACC AAGGTTGCAC TGATGTGTTG 
AAGACTGAGT TACGGGCTAC TTATAGCCAG TTGTGCAAAG ATGATATGCC AATGGTGCGA 
AGAGCTGCTG CATCTAACCT GGGGAAATTT GCTACAACTG TCGAGTCTAC CTTTTTGATT 
GCTGAGATCA TGACTATGTT CGATGATCTT ACTAAAGATG ACCAAGATTC TGTGAGACTA 
TTGGCTGTTG AAGGGTGTGC AGCTCTTGGA AAGTTGTTGG AACCTCAGGA TTGTGTTGCA 
CGCATTTTAC CTGTTATTGT TAATTTCTCT CAGGATAAAT CTTGGAGGGT GCGCTACATG 
GTTGCTAATC AGCTATATGA ACTTTGTGAG GCAGTGGGTC CTGATTGCAC GAGGACGGAT 
TTGGTTCCAG CATATGTGAG ATTGCTAAGG GACAATGAGG CTGAAGTGCG AATAGCAGCA 
GCGGGAAAAG TGACCAAGTT CTGTCGGCTT TTGAATCCAG AGCTTGCGAT TCAGCACATC 
CTTCCTTGTG TGAAGGAATT ATCATCGGAT TCTTCTCAAC ATGTCCGCTC TGCTCTAGCT 
TCAGTAATAA TGGGGATGGC TCCTATCCTT GGGAAGGACT CAACCATTGA GCATCTGTTA 
CCAATTTTTC TTTCCCTTTT GAAAGATGAA TTTCCTGATG TGCGCCTTAA CATCATAAGC 
AAGTTAGATC AAGTCAACCA GGTTATTGGA ATTGATCTAC TATCTCAATC CTTGTTACCG 
GCCATTGTAG AACTTGCTGA GGATCGGCAC TGGAGAGTCC GACTTGCAAT AATAGAGTAT 
GTTCCACTGT TGGCCAGCCA GTTAGGTATA GGATTTTTCG ATGACAAGCT CGGAGCCCTT 
TGCATGCAAT GGCTGCAAGA CAAGGTCTAC TCTATCCGCG AAGCTGCAGC AAACAACCTA 
AAGCGCCTCG CAGAGGAGTT TGGTCCTGAA TGGGCAATGC AGCACTTAGT TCCCCAGGTA 
TTGGACATGG TCAACAATCC GCACTACCTA CACAGGATGA TGGTTCTACG CGCAATATCT 
CTCATGGCGC CTGTAATGGG ATCAGAAATC ACATGCTCTA AGTTTCTTCC TGTGGTTGTT 
GAAGCATCAA AAGACAGAGT TCCAAACATC AAGTTCAACG TTGCCAAACT TCTGCAATCC 
CTCATCCCCA TAGTCGACCA ATCAGTGGTG GACAAAACAA TCCGTCAGTG TTTGGTGGAC 
CTGAGCGAAG ACCCTGATGT TGATGTTCGT TATTTTGCAA ATCAAGCACT TAATTCCATC 
GATGGTTCCA CAGCAGCACA ATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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RCN1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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