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PLCG2 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol PLCG2
Entrez Gene ID 100337091
Full Name phospholipase C gamma 2
General protein information
Preferred Names

phospholipase C gamma 2

Names

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015467274.1 XP_015322760.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
XM_024978892.1 XP_024834660.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
XM_010814382.2 XP_010812684.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2

Pathways from BioSystems


Homologies


Gallus gallus (chicken) PLCG2 XP_004944314.1
Homo sapiens (human) PLCG2 NP_002652.2
Pan troglodytes (chimpanzee) PLCG2 XP_001148089.1
PLCG2 XP_001111717.1
Mus musculus (house mouse) Plcg2 NP_758489.1
Arabidopsis thaliana (thale cress) PLC5 NP_200677.2
Xenopus tropicalis (tropical clawed frog) plcg2 XP_002934994.2
Bos taurus (cattle) PLCG2 XP_003587251.2
Danio rerio (zebrafish) plcg2 NP_001038433.1
Canis lupus familiaris (dog) PLCG2 XP_005620713.1
Rattus norvegicus (Norway rat) Plcg2 NP_058864.1

Gene Ontology

The following PLCG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225422 XM_024978892.1
Latest version!		 Bos taurus phospholipase C gamma 2 (PLCG2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa60130 XM_010814382.2
Latest version!		 Bos taurus phospholipase C gamma 2 (PLCG2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa60130 XM_015467274.1
Latest version!		 Bos taurus phospholipase C gamma 2 (PLCG2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa225422
Clone ID Related Accession (Same CDS sequence) XM_024978892.1
Accession Version XM_024978892.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGAGTTACC CTGTTTCTGC AGCCTCCTTG TTCACCCGCT TCCTCCTCGC CTCCCCCACT 
GGGCAGCAGA CGACGGCGAG CACCATGGCC AACGTGGACA CCCTCCCGGA GTATGAGAAG 
AGTCAGATCA AGCGGGCCCT GGAGCTGGGC ACCGTCATGA CCGTGTTCAG CTTCCGCAAG 
TCCACCCCGG AGCGGAGGAC CGTCCAGGTG ATCATGGAGA CGCGGCAGGT GGCCTGGAGC 
AAGACGGCCG ACAAGATCGA GGGCTTCCTG GACATCATGG AGATAAAGGA GATCCGGCCG 
GGGAAGAGCT CCAAGGATTT CGAGCGCGCG AAAGCGGTCC GCCAGAAGGA AGAATGCTGC 
TTCACCGTCC TGTATGGCTC CCAGTTTATC CTCAGCACGC TCAGCCTGGC AGCCGACTCG 
AAGGAGGATG CCGCTAAGTG GCTGTCTGGC CTGAAGATCT TACACCAGGA AGTGATGAGC 
GCGTCCACCC CCACCATCAT CGAGAGCTGG CTGAGAAAGC AGATTTATTC TGTGGATCAA 
ACCAGGAGAA ACAGCATCAG CCTCCGGGAA TTGAAGACCA TCTTGCCCCT GGTCAACTTC 
AAAGTGAGCA GTGCCAAGTT CCTCAAAGAT AAGTTCCTGG AAATAGGCGC ACACAAAGAT 
GAACTCAGCT TTGAACAGTT CCATCTCTTC TATAAAAAAC TTATGTTTGA ACAGCAAAAA 
TCGATCCTTG ACGAATTCAA AAAGGATTCT TCCGTGTTCC TCTTGGGCAA CACCGACCGG 
CCAGACGCCT CTGCTGTCCA CCTGCACGAC TTTCAGCGGT TTCTCTTGCA TGAACAGCAG 
GAGCTTTGGG CTCAGGATCT GAACAAAGTC CGTGAGCGGA TGACAAAGTT TATTGACGAC 
ACCATGAGGG AAACTGCTGA ACCCTTCCTG TTTGTGGATG AGTTCCTCAC GTACCTGTTT 
TCCCGGGAGA ACAGCATCTG GGACGAGAAG TACGACGTGG TGGACATGCA GGACATGAAC 
AACCCCTTGT CTCATTACTG GATCTCCTCG TCGCACAACA CGTACCTCAC GGGGGACCAG 
CTGCGGAGTG AGTCGTCCAC GGAGGCCTAC ATCCGCTGCC TGCGGATGGG CTGCCGCTGC 
ATCGAGCTGG ACTGCTGGGA CGGGCCCGAT GGCAAGCCCA TCATCTACCA CGGCTGGACG 
CGGACCACCA AGATCAAGTT CGACGACGTT GTGCAGGCCA TCAAAGACCA CGCCTTTGTC 
ACCTCCAGCT TCCCCGTGAT CCTGTCCATC GAGGAGCACT GCTGTGTGGA GCAGCAGCGC 
CACATGGCCA GGGTGTTCAA GGAAGTGTTT GGAGACCTGC TGCTGACCAA ACCCAAGGAG 
GCCAGTGCCG ACCAGCTGCC GTCGCCCAGC CAGCTGCGCG GGAAGATCAT CATCAAGCAT 
AAGAAGCTGG GCCCCCGCGG TGACGTGGAC GTCAACATGG AGGACAAGAA GGACGAGCAC 
AAGCAGCAGG GCGAACTGCA CATGTGGGAC CCCATCGACC AGAAATGGAC TCGGCACTAC 
TGTGCTGTTG CTGATGCCAA GCTGTCCTTC AGCGATGACA TTGAACAGAC TGTGGAAGAG 
GAGCTGCCCC TGGATATACC CCCAACAGAG CTGCATTTTG GGGAGAAGTG GTTCCACAAG 
AAGGTGGAGA AGAGGACAAG TGCCGAGAAG CTGCTACAGG AATACTGCGC CGAGACAGGG 
GGCAAGGATG GGACCTTCTT GGTGCGGGAG AGTGAGACCT TCCCCAACGA CTACACCTTG 
TCCTTCTGGC GGTCGGGCCG CGTGCAGCAC TGCCGGATCC GCTCCACCAT GGAGGGTGGG 
ACCATGAAGT ACTACCTGAC CGACAACCTC ATGTTCACCA GCATCTACGC CCTCATCCAG 
CACTACCGAG AGACACACCT GCGCTGTGCC GAGTTCGAGC TCCGGCTGAC CGACCCCGTG 
CCCAACCCCA ACCCGCACGA GTCCAAGCCG TGGTACTACG ATGGGCTGAG CCGTGGAGAA 
GCGGAGGACA TGCTGATGCG GATCCCCCGG GACGGGGCCT TCCTGATTCG GAAGCGGGAA 
GGTACCGACT CCTACGCCAT CACCTTCAGG GCCAGGGGCA AGGTGAAGCA CTGTCGCATC 
AACCGAGACG GCCGGCACTT CGTGCTGGGC ACCTCCGCCT ACTTCGAGAG CCTCGTGGAG 
CTGGTCAGTT ACTACGAGAA GCACGCGCTC TACCGGAAGA TGAAGCTGCG CTACCCCGTG 
ACCCCCGAGC TCCTGGAGCG CTACAACACG GAAAGAGATA TAAACTCCCT CTACGATGTC 
AGCAGGATGT ATGTGGATCC CAGTGAAATC AATCCTTCAA TGCCTCAGAG AACCGTGAAA 
GCTCTGTATG ACTACAGGGC CAAGCAGAGT GACGAGCTGA GCTTTTGCCG CGGTGCCCTC 
ATCCACAACG TCTCCAAGGA GCCTGGGGGC TGGTGGAAAG GAGACTATGG GACCAGAATC 
CAGCAGTACT TCCCGTCCAA TTACGTCGAG GACATTTCAC CAACAGAAGG GGAGGAGGTG 
GATAAGCAGA TTATTGAAGA CAATCCCTTA GGGTCCCTTT GCAGAGGAAT CCTGAATCTC 
AACATCTATA ATGTTGTAAA AGCCCCACAT GGGAAGAACC AGAAGCCTTT CGTCTTTATC 
TTGGAGCCCA AGAAGCAGGG CGACCCTCCG GTAGAGTTTG CCACGGACAG CGTGGAGGAG 
CTGTTTGAGT GGTTCCAGAG CATCCGGGAG ATCACCTGGA AGATGGACGC CAAGGAGAAC 
AACATGAAGT ACTGGGAGAA GAACCAGTCC ATCGCCATCG AGCTCTCGGA CCTGGTCGTC 
TACTGCAAGC CAACCAGCAA GACCAAGGAC AACCTCGAAA ACCCTGACTT CCGAGAAATC 
CGCTCCTTCG TGGAGACGAA GGCTGACAGT ATCGTCAGAC AGAAGCCCAG CGATCTCCTG 
AAATACAATC AAAAGGGCCT GACCCGTGTG TACCCCAAAG GACAGCGGGT GGACTCGTCC 
AACTATGACC CCTTCCGCCT CTGGCTGTGC GGCTCCCAGA TGGTGGCACT CAATTTCCAG 
ACTCCAGATA AGTACATGCA GATGAACCAC GCCCTGTTCT CCCTCAACGG GCGGACGGGC 
TACGTCCTGC AGCCTGAGAG CATGAGGGCG GAGAAGTACG ACCCCATGCC ACCCGAGTCC 
CAGAGGAAGA TCCAGATGAC CCTGACGGTC AAGGTGCTGG GGGCCCGTCA CCTCCCCAAG 
CCCGGCCGCA GCATCGCGTG CCCCTTCGTG GAGGTGGAGA TATGCGGAGC GGAGTACGAC 
AGCAACAAGT TTAAGACGAC GGTTGTGAAT GACAATGGCC TCAGCCCTGT TTGGACCCCA 
ACGCAGGAGA AGGTGACGTT TGAGATTTAT GACCCCAACC TTGCATTTCT GCGCTTTGTG 
GTTTATGAGG AAGATATGTT CAGCGATCCC AACTTTCTGG CCCATGCCAC GTACCCCATT 
AAAGGAATCA AGTCAGGATT TAGATCTGTT CCTCTGAAGA ACGGCTACAG TGAAGACATT 
GAGCTGGCGT CCCTCCTGGT TTTCTGTGAG ATGCGGCCAG TCCTGGAGAG CGAAGAGGAG 
CTGTATTCCT CCTGCCGCCA GCTGCGGAGG CGGCAAGAAG AACTGAACAA CCAGCTCTTT 
CTGTACGACA CCCACCAGAA CCTGCGCAGC GCCACCCGCG ATGCCCTGGT CAGAGAATTC 
AACGTCAACG AGAACCAGCT GCAGCTGTAC CAGGAGAAGT GCAACCGCAG GTTAAGAGAG 
AAGAGAGTCA GCAACAGCAA GTTTTACTCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024834660.1
CDS16..3888
Translation

Target ORF information:

RefSeq Version XM_024978892.1
Organism Bos taurus(cattle)
Definition Bos taurus phospholipase C gamma 2 (PLCG2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024978892.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGAGTTACC CTGTTTCTGC AGCCTCCTTG TTCACCCGCT TCCTCCTCGC CTCCCCCACT 
GGGCAGCAGA CGACGGCGAG CACCATGGCC AACGTGGACA CCCTCCCGGA GTATGAGAAG 
AGTCAGATCA AGCGGGCCCT GGAGCTGGGC ACCGTCATGA CCGTGTTCAG CTTCCGCAAG 
TCCACCCCGG AGCGGAGGAC CGTCCAGGTG ATCATGGAGA CGCGGCAGGT GGCCTGGAGC 
AAGACGGCCG ACAAGATCGA GGGCTTCCTG GACATCATGG AGATAAAGGA GATCCGGCCG 
GGGAAGAGCT CCAAGGATTT CGAGCGCGCG AAAGCGGTCC GCCAGAAGGA AGAATGCTGC 
TTCACCGTCC TGTATGGCTC CCAGTTTATC CTCAGCACGC TCAGCCTGGC AGCCGACTCG 
AAGGAGGATG CCGCTAAGTG GCTGTCTGGC CTGAAGATCT TACACCAGGA AGTGATGAGC 
GCGTCCACCC CCACCATCAT CGAGAGCTGG CTGAGAAAGC AGATTTATTC TGTGGATCAA 
ACCAGGAGAA ACAGCATCAG CCTCCGGGAA TTGAAGACCA TCTTGCCCCT GGTCAACTTC 
AAAGTGAGCA GTGCCAAGTT CCTCAAAGAT AAGTTCCTGG AAATAGGCGC ACACAAAGAT 
GAACTCAGCT TTGAACAGTT CCATCTCTTC TATAAAAAAC TTATGTTTGA ACAGCAAAAA 
TCGATCCTTG ACGAATTCAA AAAGGATTCT TCCGTGTTCC TCTTGGGCAA CACCGACCGG 
CCAGACGCCT CTGCTGTCCA CCTGCACGAC TTTCAGCGGT TTCTCTTGCA TGAACAGCAG 
GAGCTTTGGG CTCAGGATCT GAACAAAGTC CGTGAGCGGA TGACAAAGTT TATTGACGAC 
ACCATGAGGG AAACTGCTGA ACCCTTCCTG TTTGTGGATG AGTTCCTCAC GTACCTGTTT 
TCCCGGGAGA ACAGCATCTG GGACGAGAAG TACGACGTGG TGGACATGCA GGACATGAAC 
AACCCCTTGT CTCATTACTG GATCTCCTCG TCGCACAACA CGTACCTCAC GGGGGACCAG 
CTGCGGAGTG AGTCGTCCAC GGAGGCCTAC ATCCGCTGCC TGCGGATGGG CTGCCGCTGC 
ATCGAGCTGG ACTGCTGGGA CGGGCCCGAT GGCAAGCCCA TCATCTACCA CGGCTGGACG 
CGGACCACCA AGATCAAGTT CGACGACGTT GTGCAGGCCA TCAAAGACCA CGCCTTTGTC 
ACCTCCAGCT TCCCCGTGAT CCTGTCCATC GAGGAGCACT GCTGTGTGGA GCAGCAGCGC 
CACATGGCCA GGGTGTTCAA GGAAGTGTTT GGAGACCTGC TGCTGACCAA ACCCAAGGAG 
GCCAGTGCCG ACCAGCTGCC GTCGCCCAGC CAGCTGCGCG GGAAGATCAT CATCAAGCAT 
AAGAAGCTGG GCCCCCGCGG TGACGTGGAC GTCAACATGG AGGACAAGAA GGACGAGCAC 
AAGCAGCAGG GCGAACTGCA CATGTGGGAC CCCATCGACC AGAAATGGAC TCGGCACTAC 
TGTGCTGTTG CTGATGCCAA GCTGTCCTTC AGCGATGACA TTGAACAGAC TGTGGAAGAG 
GAGCTGCCCC TGGATATACC CCCAACAGAG CTGCATTTTG GGGAGAAGTG GTTCCACAAG 
AAGGTGGAGA AGAGGACAAG TGCCGAGAAG CTGCTACAGG AATACTGCGC CGAGACAGGG 
GGCAAGGATG GGACCTTCTT GGTGCGGGAG AGTGAGACCT TCCCCAACGA CTACACCTTG 
TCCTTCTGGC GGTCGGGCCG CGTGCAGCAC TGCCGGATCC GCTCCACCAT GGAGGGTGGG 
ACCATGAAGT ACTACCTGAC CGACAACCTC ATGTTCACCA GCATCTACGC CCTCATCCAG 
CACTACCGAG AGACACACCT GCGCTGTGCC GAGTTCGAGC TCCGGCTGAC CGACCCCGTG 
CCCAACCCCA ACCCGCACGA GTCCAAGCCG TGGTACTACG ATGGGCTGAG CCGTGGAGAA 
GCGGAGGACA TGCTGATGCG GATCCCCCGG GACGGGGCCT TCCTGATTCG GAAGCGGGAA 
GGTACCGACT CCTACGCCAT CACCTTCAGG GCCAGGGGCA AGGTGAAGCA CTGTCGCATC 
AACCGAGACG GCCGGCACTT CGTGCTGGGC ACCTCCGCCT ACTTCGAGAG CCTCGTGGAG 
CTGGTCAGTT ACTACGAGAA GCACGCGCTC TACCGGAAGA TGAAGCTGCG CTACCCCGTG 
ACCCCCGAGC TCCTGGAGCG CTACAACACG GAAAGAGATA TAAACTCCCT CTACGATGTC 
AGCAGGATGT ATGTGGATCC CAGTGAAATC AATCCTTCAA TGCCTCAGAG AACCGTGAAA 
GCTCTGTATG ACTACAGGGC CAAGCAGAGT GACGAGCTGA GCTTTTGCCG CGGTGCCCTC 
ATCCACAACG TCTCCAAGGA GCCTGGGGGC TGGTGGAAAG GAGACTATGG GACCAGAATC 
CAGCAGTACT TCCCGTCCAA TTACGTCGAG GACATTTCAC CAACAGAAGG GGAGGAGGTG 
GATAAGCAGA TTATTGAAGA CAATCCCTTA GGGTCCCTTT GCAGAGGAAT CCTGAATCTC 
AACATCTATA ATGTTGTAAA AGCCCCACAT GGGAAGAACC AGAAGCCTTT CGTCTTTATC 
TTGGAGCCCA AGAAGCAGGG CGACCCTCCG GTAGAGTTTG CCACGGACAG CGTGGAGGAG 
CTGTTTGAGT GGTTCCAGAG CATCCGGGAG ATCACCTGGA AGATGGACGC CAAGGAGAAC 
AACATGAAGT ACTGGGAGAA GAACCAGTCC ATCGCCATCG AGCTCTCGGA CCTGGTCGTC 
TACTGCAAGC CAACCAGCAA GACCAAGGAC AACCTCGAAA ACCCTGACTT CCGAGAAATC 
CGCTCCTTCG TGGAGACGAA GGCTGACAGT ATCGTCAGAC AGAAGCCCAG CGATCTCCTG 
AAATACAATC AAAAGGGCCT GACCCGTGTG TACCCCAAAG GACAGCGGGT GGACTCGTCC 
AACTATGACC CCTTCCGCCT CTGGCTGTGC GGCTCCCAGA TGGTGGCACT CAATTTCCAG 
ACTCCAGATA AGTACATGCA GATGAACCAC GCCCTGTTCT CCCTCAACGG GCGGACGGGC 
TACGTCCTGC AGCCTGAGAG CATGAGGGCG GAGAAGTACG ACCCCATGCC ACCCGAGTCC 
CAGAGGAAGA TCCAGATGAC CCTGACGGTC AAGGTGCTGG GGGCCCGTCA CCTCCCCAAG 
CCCGGCCGCA GCATCGCGTG CCCCTTCGTG GAGGTGGAGA TATGCGGAGC GGAGTACGAC 
AGCAACAAGT TTAAGACGAC GGTTGTGAAT GACAATGGCC TCAGCCCTGT TTGGACCCCA 
ACGCAGGAGA AGGTGACGTT TGAGATTTAT GACCCCAACC TTGCATTTCT GCGCTTTGTG 
GTTTATGAGG AAGATATGTT CAGCGATCCC AACTTTCTGG CCCATGCCAC GTACCCCATT 
AAAGGAATCA AGTCAGGATT TAGATCTGTT CCTCTGAAGA ACGGCTACAG TGAAGACATT 
GAGCTGGCGT CCCTCCTGGT TTTCTGTGAG ATGCGGCCAG TCCTGGAGAG CGAAGAGGAG 
CTGTATTCCT CCTGCCGCCA GCTGCGGAGG CGGCAAGAAG AACTGAACAA CCAGCTCTTT 
CTGTACGACA CCCACCAGAA CCTGCGCAGC GCCACCCGCG ATGCCCTGGT CAGAGAATTC 
AACGTCAACG AGAACCAGCT GCAGCTGTAC CAGGAGAAGT GCAACCGCAG GTTAAGAGAG 
AAGAGAGTCA GCAACAGCAA GTTTTACTCA TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa60130
Clone ID Related Accession (Same CDS sequence) XM_015467274.1 , XM_010814382.2
Accession Version XM_015467274.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646380.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCGAGCA CCATGGCCAA CGTGGACACC CTCCCGGAGT ATGAGAAGAG TCAGATCAAG 
CGGGCCCTGG AGCTGGGCAC CGTCATGACC GTGTTCAGCT TCCGCAAGTC CACCCCGGAG 
CGGAGGACCG TCCAGGTGAT CATGGAGACG CGGCAGGTGG CCTGGAGCAA GACGGCCGAC 
AAGATCGAGG GCTTCCTGGA CATCATGGAG ATAAAGGAGA TCCGGCCGGG GAAGAGCTCC 
AAGGATTTCG AGCGCGCGAA AGCGGTCCGC CAGAAGGAAG AATGCTGCTT CACCGTCCTG 
TATGGCTCCC AGTTTATCCT CAGCACGCTC AGCCTGGCAG CCGACTCGAA GGAGGATGCC 
GCTAAGTGGC TGTCTGGCCT GAAGATCTTA CACCAGGAAG TGATGAGCGC GTCCACCCCC 
ACCATCATCG AGAGCTGGCT GAGAAAGCAG ATTTATTCTG TGGATCAAAC CAGGAGAAAC 
AGCATCAGCC TCCGGGAATT GAAGACCATC TTGCCCCTGG TCAACTTCAA AGTGAGCAGT 
GCCAAGTTCC TCAAAGATAA GTTCCTGGAA ATAGGCGCAC ACAAAGATGA ACTCAGCTTT 
GAACAGTTCC ATCTCTTCTA TAAAAAACTT ATGTTTGAAC AGCAAAAATC GATCCTTGAC 
GAATTCAAAA AGGATTCTTC CGTGTTCCTC TTGGGCAACA CCGACCGGCC AGACGCCTCT 
GCTGTCCACC TGCACGACTT TCAGCGGTTT CTCTTGCATG AACAGCAGGA GCTTTGGGCT 
CAGGATCTGA ACAAAGTCCG TGAGCGGATG ACAAAGTTTA TTGACGACAC CATGAGGGAA 
ACTGCTGAAC CCTTCCTGTT TGTGGATGAG TTCCTCACGT ACCTGTTTTC CCGGGAGAAC 
AGCATCTGGG ACGAGAAGTA CGACGTGGTG GACATGCAGG ACATGAACAA CCCCTTGTCT 
CATTACTGGA TCTCCTCGTC GCACAACACG TACCTCACGG GGGACCAGCT GCGGAGTGAG 
TCGTCCACGG AGGCCTACAT CCGCTGCCTG CGGATGGGCT GCCGCTGCAT CGAGCTGGAC 
TGCTGGGACG GGCCCGATGG CAAGCCCATC ATCTACCACG GCTGGACGCG GACCACCAAG 
ATCAAGTTCG ACGACGTTGT GCAGGCCATC AAAGACCACG CCTTTGTCAC CTCCAGCTTC 
CCCGTGATCC TGTCCATCGA GGAGCACTGC TGTGTGGAGC AGCAGCGCCA CATGGCCAGG 
GTGTTCAAGG AAGTGTTTGG AGACCTGCTG CTGACCAAAC CCAAGGAGGC CAGTGCCGAC 
CAGCTGCCGT CGCCCAGCCA GCTGCGCGGG AAGATCATCA TCAAGCATAA GAAGCTGGGC 
CCCCGCGGTG ACGTGGACGT CAACATGGAG GACAAGAAGG ACGAGCACAA GCAGCAGGGC 
GAACTGCACA TGTGGGACCC CATCGACCAG AAATGGACTC GGCACTACTG TGCTGTTGCT 
GATGCCAAGC TGTCCTTCAG CGATGACATT GAACAGACTG TGGAAGAGGA GCTGCCCCTG 
GATATACCCC CAACAGAGCT GCATTTTGGG GAGAAGTGGT TCCACAAGAA GGTGGAGAAG 
AGGACAAGTG CCGAGAAGCT GCTACAGGAA TACTGCGCCG AGACAGGGGG CAAGGATGGG 
ACCTTCTTGG TGCGGGAGAG TGAGACCTTC CCCAACGACT ACACCTTGTC CTTCTGGCGG 
TCGGGCCGCG TGCAGCACTG CCGGATCCGC TCCACCATGG AGGGTGGGAC CATGAAGTAC 
TACCTGACCG ACAACCTCAT GTTCACCAGC ATCTACGCCC TCATCCAGCA CTACCGAGAG 
ACACACCTGC GCTGTGCCGA GTTCGAGCTC CGGCTGACCG ACCCCGTGCC CAACCCCAAC 
CCGCACGAGT CCAAGCCGTG GTACTACGAT GGGCTGAGCC GTGGAGAAGC GGAGGACATG 
CTGATGCGGA TCCCCCGGGA CGGGGCCTTC CTGATTCGGA AGCGGGAAGG TACCGACTCC 
TACGCCATCA CCTTCAGGGC CAGGGGCAAG GTGAAGCACT GTCGCATCAA CCGAGACGGC 
CGGCACTTCG TGCTGGGCAC CTCCGCCTAC TTCGAGAGCC TCGTGGAGCT GGTCAGTTAC 
TACGAGAAGC ACGCGCTCTA CCGGAAGATG AAGCTGCGCT ACCCCGTGAC CCCCGAGCTC 
CTGGAGCGCT ACAACACGGA AAGAGATATA AACTCCCTCT ACGATGTCAG CAGGATGTAT 
GTGGATCCCA GTGAAATCAA TCCTTCAATG CCTCAGAGAA CCGTGAAAGC TCTGTATGAC 
TACAGGGCCA AGCAGAGTGA CGAGCTGAGC TTTTGCCGCG GTGCCCTCAT CCACAACGTC 
TCCAAGGAGC CTGGGGGCTG GTGGAAAGGA GACTATGGGA CCAGAATCCA GCAGTACTTC 
CCGTCCAATT ACGTCGAGGA CATTTCACCA ACAGAAGGGG AGGAGGTGGA TAAGCAGATT 
ATTGAAGACA ATCCCTTAGG GTCCCTTTGC AGAGGAATCC TGAATCTCAA CATAAGGAAT 
GTTGTAAAAG CCCCACATGG GAAGAACCAG AAGCCTTTCG TCTTTATCTT GGAGCCCAAG 
AAGCAGGGCG ACCCTCCGGT AGAGTTTGCC ACGGACAGCG TGGAGGAGCT GTTTGAGTGG 
TTCCAGAGCA TCCGGGAGAT CACCTGGAAG ATGGACGCCA AGGAGAACAA CATGAAGTAC 
TGGGAGAAGA ACCAGTCCAT CGCCATCGAG CTCTCGGACC TGGTCGTCTA CTGCAAGCCA 
ACCAGCAAGA CCAAGGACAA CCTCGAAAAC CCTGACTTCC GAGAAATCCG CTCCTTCGTG 
GAGACGAAGG CTGACAGTAT CGTCAGACAG AAGCCCAGCG ATCTCCTGAA ATACAATCAA 
AAGGGCCTGA CCCGTGTGTA CCCCAAAGGA CAGCGGGTGG ACTCGTCCAA CTATGACCCC 
TTCCGCCTCT GGCTGTGCGG CTCCCAGATG GTGGCACTCA ATTTCCAGAC TCCAGATAAG 
TACATGCAGA TGAACCACGC CCTGTTCTCC CTCAACGGGC GGACGGGCTA CGTCCTGCAG 
CCTGAGAGCA TGAGGGCGGA GAAGTACGAC CCCATGCCAC CCGAGTCCCA GAGGAAGATC 
CAGATGACCC TGACGGTCAA GGTGCTGGGG GCCCGTCACC TCCCCAAGCC CGGCCGCAGC 
ATCGCGTGCC CCTTCGTGGA GGTGGAGATA TGCGGAGCGG AGTACGACAG CAACAAGTTT 
AAGACGACGG TTGTGAATGA CAATGGCCTC AGCCCTGTTT GGACCCCAAC GCAGGAGAAG 
GTGACGTTTG AGATTTATGA CCCCAACCTT GCATTTCTGC GCTTTGTGGT TTATGAGGAA 
GATATGTTCA GCGATCCCAA CTTTCTGGCC CATGCCACGT ACCCCATTAA AGGAATCAAG 
TCAGGATTTA GATCTGTTCC TCTGAAGAAC GGCTACAGTG AAGACATTGA GCTGGCGTCC 
CTCCTGGTTT TCTGTGAGAT GCGGCCAGTC CTGGAGAGCG AAGAGGAGCT GTATTCCTCC 
TGCCGCCAGC TGCGGAGGCG GCAAGAAGAA CTGAACAACC AGCTCTTTCT GTACGACACC 
CACCAGAACC TGCGCAGCGC CACCCGCGAT GCCCTGGTCA GAGAATTCAA CGTCAACGAG 
AACCAGCTGC AGCTGTACCA GGAGAAGTGC AACCGCAGGT TAAGAGAGAA GAGAGTCAGC 
AACAGCAAGT TTTACTCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015322760.1
CDS166..3966
Translation

Target ORF information:

RefSeq Version XM_015467274.1
Organism Bos taurus(cattle)
Definition Bos taurus phospholipase C gamma 2 (PLCG2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015467274.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCGAGCA CCATGGCCAA CGTGGACACC CTCCCGGAGT ATGAGAAGAG TCAGATCAAG 
CGGGCCCTGG AGCTGGGCAC CGTCATGACC GTGTTCAGCT TCCGCAAGTC CACCCCGGAG 
CGGAGGACCG TCCAGGTGAT CATGGAGACG CGGCAGGTGG CCTGGAGCAA GACGGCCGAC 
AAGATCGAGG GCTTCCTGGA CATCATGGAG ATAAAGGAGA TCCGGCCGGG GAAGAGCTCC 
AAGGATTTCG AGCGCGCGAA AGCGGTCCGC CAGAAGGAAG AATGCTGCTT CACCGTCCTG 
TATGGCTCCC AGTTTATCCT CAGCACGCTC AGCCTGGCAG CCGACTCGAA GGAGGATGCC 
GCTAAGTGGC TGTCTGGCCT GAAGATCTTA CACCAGGAAG TGATGAGCGC GTCCACCCCC 
ACCATCATCG AGAGCTGGCT GAGAAAGCAG ATTTATTCTG TGGATCAAAC CAGGAGAAAC 
AGCATCAGCC TCCGGGAATT GAAGACCATC TTGCCCCTGG TCAACTTCAA AGTGAGCAGT 
GCCAAGTTCC TCAAAGATAA GTTCCTGGAA ATAGGCGCAC ACAAAGATGA ACTCAGCTTT 
GAACAGTTCC ATCTCTTCTA TAAAAAACTT ATGTTTGAAC AGCAAAAATC GATCCTTGAC 
GAATTCAAAA AGGATTCTTC CGTGTTCCTC TTGGGCAACA CCGACCGGCC AGACGCCTCT 
GCTGTCCACC TGCACGACTT TCAGCGGTTT CTCTTGCATG AACAGCAGGA GCTTTGGGCT 
CAGGATCTGA ACAAAGTCCG TGAGCGGATG ACAAAGTTTA TTGACGACAC CATGAGGGAA 
ACTGCTGAAC CCTTCCTGTT TGTGGATGAG TTCCTCACGT ACCTGTTTTC CCGGGAGAAC 
AGCATCTGGG ACGAGAAGTA CGACGTGGTG GACATGCAGG ACATGAACAA CCCCTTGTCT 
CATTACTGGA TCTCCTCGTC GCACAACACG TACCTCACGG GGGACCAGCT GCGGAGTGAG 
TCGTCCACGG AGGCCTACAT CCGCTGCCTG CGGATGGGCT GCCGCTGCAT CGAGCTGGAC 
TGCTGGGACG GGCCCGATGG CAAGCCCATC ATCTACCACG GCTGGACGCG GACCACCAAG 
ATCAAGTTCG ACGACGTTGT GCAGGCCATC AAAGACCACG CCTTTGTCAC CTCCAGCTTC 
CCCGTGATCC TGTCCATCGA GGAGCACTGC TGTGTGGAGC AGCAGCGCCA CATGGCCAGG 
GTGTTCAAGG AAGTGTTTGG AGACCTGCTG CTGACCAAAC CCAAGGAGGC CAGTGCCGAC 
CAGCTGCCGT CGCCCAGCCA GCTGCGCGGG AAGATCATCA TCAAGCATAA GAAGCTGGGC 
CCCCGCGGTG ACGTGGACGT CAACATGGAG GACAAGAAGG ACGAGCACAA GCAGCAGGGC 
GAACTGCACA TGTGGGACCC CATCGACCAG AAATGGACTC GGCACTACTG TGCTGTTGCT 
GATGCCAAGC TGTCCTTCAG CGATGACATT GAACAGACTG TGGAAGAGGA GCTGCCCCTG 
GATATACCCC CAACAGAGCT GCATTTTGGG GAGAAGTGGT TCCACAAGAA GGTGGAGAAG 
AGGACAAGTG CCGAGAAGCT GCTACAGGAA TACTGCGCCG AGACAGGGGG CAAGGATGGG 
ACCTTCTTGG TGCGGGAGAG TGAGACCTTC CCCAACGACT ACACCTTGTC CTTCTGGCGG 
TCGGGCCGCG TGCAGCACTG CCGGATCCGC TCCACCATGG AGGGTGGGAC CATGAAGTAC 
TACCTGACCG ACAACCTCAT GTTCACCAGC ATCTACGCCC TCATCCAGCA CTACCGAGAG 
ACACACCTGC GCTGTGCCGA GTTCGAGCTC CGGCTGACCG ACCCCGTGCC CAACCCCAAC 
CCGCACGAGT CCAAGCCGTG GTACTACGAT GGGCTGAGCC GTGGAGAAGC GGAGGACATG 
CTGATGCGGA TCCCCCGGGA CGGGGCCTTC CTGATTCGGA AGCGGGAAGG TACCGACTCC 
TACGCCATCA CCTTCAGGGC CAGGGGCAAG GTGAAGCACT GTCGCATCAA CCGAGACGGC 
CGGCACTTCG TGCTGGGCAC CTCCGCCTAC TTCGAGAGCC TCGTGGAGCT GGTCAGTTAC 
TACGAGAAGC ACGCGCTCTA CCGGAAGATG AAGCTGCGCT ACCCCGTGAC CCCCGAGCTC 
CTGGAGCGCT ACAACACGGA AAGAGATATA AACTCCCTCT ACGATGTCAG CAGGATGTAT 
GTGGATCCCA GTGAAATCAA TCCTTCAATG CCTCAGAGAA CCGTGAAAGC TCTGTATGAC 
TACAGGGCCA AGCAGAGTGA CGAGCTGAGC TTTTGCCGCG GTGCCCTCAT CCACAACGTC 
TCCAAGGAGC CTGGGGGCTG GTGGAAAGGA GACTATGGGA CCAGAATCCA GCAGTACTTC 
CCGTCCAATT ACGTCGAGGA CATTTCACCA ACAGAAGGGG AGGAGGTGGA TAAGCAGATT 
ATTGAAGACA ATCCCTTAGG GTCCCTTTGC AGAGGAATCC TGAATCTCAA CATAAGGAAT 
GTTGTAAAAG CCCCACATGG GAAGAACCAG AAGCCTTTCG TCTTTATCTT GGAGCCCAAG 
AAGCAGGGCG ACCCTCCGGT AGAGTTTGCC ACGGACAGCG TGGAGGAGCT GTTTGAGTGG 
TTCCAGAGCA TCCGGGAGAT CACCTGGAAG ATGGACGCCA AGGAGAACAA CATGAAGTAC 
TGGGAGAAGA ACCAGTCCAT CGCCATCGAG CTCTCGGACC TGGTCGTCTA CTGCAAGCCA 
ACCAGCAAGA CCAAGGACAA CCTCGAAAAC CCTGACTTCC GAGAAATCCG CTCCTTCGTG 
GAGACGAAGG CTGACAGTAT CGTCAGACAG AAGCCCAGCG ATCTCCTGAA ATACAATCAA 
AAGGGCCTGA CCCGTGTGTA CCCCAAAGGA CAGCGGGTGG ACTCGTCCAA CTATGACCCC 
TTCCGCCTCT GGCTGTGCGG CTCCCAGATG GTGGCACTCA ATTTCCAGAC TCCAGATAAG 
TACATGCAGA TGAACCACGC CCTGTTCTCC CTCAACGGGC GGACGGGCTA CGTCCTGCAG 
CCTGAGAGCA TGAGGGCGGA GAAGTACGAC CCCATGCCAC CCGAGTCCCA GAGGAAGATC 
CAGATGACCC TGACGGTCAA GGTGCTGGGG GCCCGTCACC TCCCCAAGCC CGGCCGCAGC 
ATCGCGTGCC CCTTCGTGGA GGTGGAGATA TGCGGAGCGG AGTACGACAG CAACAAGTTT 
AAGACGACGG TTGTGAATGA CAATGGCCTC AGCCCTGTTT GGACCCCAAC GCAGGAGAAG 
GTGACGTTTG AGATTTATGA CCCCAACCTT GCATTTCTGC GCTTTGTGGT TTATGAGGAA 
GATATGTTCA GCGATCCCAA CTTTCTGGCC CATGCCACGT ACCCCATTAA AGGAATCAAG 
TCAGGATTTA GATCTGTTCC TCTGAAGAAC GGCTACAGTG AAGACATTGA GCTGGCGTCC 
CTCCTGGTTT TCTGTGAGAT GCGGCCAGTC CTGGAGAGCG AAGAGGAGCT GTATTCCTCC 
TGCCGCCAGC TGCGGAGGCG GCAAGAAGAA CTGAACAACC AGCTCTTTCT GTACGACACC 
CACCAGAACC TGCGCAGCGC CACCCGCGAT GCCCTGGTCA GAGAATTCAA CGTCAACGAG 
AACCAGCTGC AGCTGTACCA GGAGAAGTGC AACCGCAGGT TAAGAGAGAA GAGAGTCAGC 
AACAGCAAGT TTTACTCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa60130
Clone ID Related Accession (Same CDS sequence) XM_015467274.1 , XM_010814382.2
Accession Version XM_010814382.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCGAGCA CCATGGCCAA CGTGGACACC CTCCCGGAGT ATGAGAAGAG TCAGATCAAG 
CGGGCCCTGG AGCTGGGCAC CGTCATGACC GTGTTCAGCT TCCGCAAGTC CACCCCGGAG 
CGGAGGACCG TCCAGGTGAT CATGGAGACG CGGCAGGTGG CCTGGAGCAA GACGGCCGAC 
AAGATCGAGG GCTTCCTGGA CATCATGGAG ATAAAGGAGA TCCGGCCGGG GAAGAGCTCC 
AAGGATTTCG AGCGCGCGAA AGCGGTCCGC CAGAAGGAAG AATGCTGCTT CACCGTCCTG 
TATGGCTCCC AGTTTATCCT CAGCACGCTC AGCCTGGCAG CCGACTCGAA GGAGGATGCC 
GCTAAGTGGC TGTCTGGCCT GAAGATCTTA CACCAGGAAG TGATGAGCGC GTCCACCCCC 
ACCATCATCG AGAGCTGGCT GAGAAAGCAG ATTTATTCTG TGGATCAAAC CAGGAGAAAC 
AGCATCAGCC TCCGGGAATT GAAGACCATC TTGCCCCTGG TCAACTTCAA AGTGAGCAGT 
GCCAAGTTCC TCAAAGATAA GTTCCTGGAA ATAGGCGCAC ACAAAGATGA ACTCAGCTTT 
GAACAGTTCC ATCTCTTCTA TAAAAAACTT ATGTTTGAAC AGCAAAAATC GATCCTTGAC 
GAATTCAAAA AGGATTCTTC CGTGTTCCTC TTGGGCAACA CCGACCGGCC AGACGCCTCT 
GCTGTCCACC TGCACGACTT TCAGCGGTTT CTCTTGCATG AACAGCAGGA GCTTTGGGCT 
CAGGATCTGA ACAAAGTCCG TGAGCGGATG ACAAAGTTTA TTGACGACAC CATGAGGGAA 
ACTGCTGAAC CCTTCCTGTT TGTGGATGAG TTCCTCACGT ACCTGTTTTC CCGGGAGAAC 
AGCATCTGGG ACGAGAAGTA CGACGTGGTG GACATGCAGG ACATGAACAA CCCCTTGTCT 
CATTACTGGA TCTCCTCGTC GCACAACACG TACCTCACGG GGGACCAGCT GCGGAGTGAG 
TCGTCCACGG AGGCCTACAT CCGCTGCCTG CGGATGGGCT GCCGCTGCAT CGAGCTGGAC 
TGCTGGGACG GGCCCGATGG CAAGCCCATC ATCTACCACG GCTGGACGCG GACCACCAAG 
ATCAAGTTCG ACGACGTTGT GCAGGCCATC AAAGACCACG CCTTTGTCAC CTCCAGCTTC 
CCCGTGATCC TGTCCATCGA GGAGCACTGC TGTGTGGAGC AGCAGCGCCA CATGGCCAGG 
GTGTTCAAGG AAGTGTTTGG AGACCTGCTG CTGACCAAAC CCAAGGAGGC CAGTGCCGAC 
CAGCTGCCGT CGCCCAGCCA GCTGCGCGGG AAGATCATCA TCAAGCATAA GAAGCTGGGC 
CCCCGCGGTG ACGTGGACGT CAACATGGAG GACAAGAAGG ACGAGCACAA GCAGCAGGGC 
GAACTGCACA TGTGGGACCC CATCGACCAG AAATGGACTC GGCACTACTG TGCTGTTGCT 
GATGCCAAGC TGTCCTTCAG CGATGACATT GAACAGACTG TGGAAGAGGA GCTGCCCCTG 
GATATACCCC CAACAGAGCT GCATTTTGGG GAGAAGTGGT TCCACAAGAA GGTGGAGAAG 
AGGACAAGTG CCGAGAAGCT GCTACAGGAA TACTGCGCCG AGACAGGGGG CAAGGATGGG 
ACCTTCTTGG TGCGGGAGAG TGAGACCTTC CCCAACGACT ACACCTTGTC CTTCTGGCGG 
TCGGGCCGCG TGCAGCACTG CCGGATCCGC TCCACCATGG AGGGTGGGAC CATGAAGTAC 
TACCTGACCG ACAACCTCAT GTTCACCAGC ATCTACGCCC TCATCCAGCA CTACCGAGAG 
ACACACCTGC GCTGTGCCGA GTTCGAGCTC CGGCTGACCG ACCCCGTGCC CAACCCCAAC 
CCGCACGAGT CCAAGCCGTG GTACTACGAT GGGCTGAGCC GTGGAGAAGC GGAGGACATG 
CTGATGCGGA TCCCCCGGGA CGGGGCCTTC CTGATTCGGA AGCGGGAAGG TACCGACTCC 
TACGCCATCA CCTTCAGGGC CAGGGGCAAG GTGAAGCACT GTCGCATCAA CCGAGACGGC 
CGGCACTTCG TGCTGGGCAC CTCCGCCTAC TTCGAGAGCC TCGTGGAGCT GGTCAGTTAC 
TACGAGAAGC ACGCGCTCTA CCGGAAGATG AAGCTGCGCT ACCCCGTGAC CCCCGAGCTC 
CTGGAGCGCT ACAACACGGA AAGAGATATA AACTCCCTCT ACGATGTCAG CAGGATGTAT 
GTGGATCCCA GTGAAATCAA TCCTTCAATG CCTCAGAGAA CCGTGAAAGC TCTGTATGAC 
TACAGGGCCA AGCAGAGTGA CGAGCTGAGC TTTTGCCGCG GTGCCCTCAT CCACAACGTC 
TCCAAGGAGC CTGGGGGCTG GTGGAAAGGA GACTATGGGA CCAGAATCCA GCAGTACTTC 
CCGTCCAATT ACGTCGAGGA CATTTCACCA ACAGAAGGGG AGGAGGTGGA TAAGCAGATT 
ATTGAAGACA ATCCCTTAGG GTCCCTTTGC AGAGGAATCC TGAATCTCAA CATAAGGAAT 
GTTGTAAAAG CCCCACATGG GAAGAACCAG AAGCCTTTCG TCTTTATCTT GGAGCCCAAG 
AAGCAGGGCG ACCCTCCGGT AGAGTTTGCC ACGGACAGCG TGGAGGAGCT GTTTGAGTGG 
TTCCAGAGCA TCCGGGAGAT CACCTGGAAG ATGGACGCCA AGGAGAACAA CATGAAGTAC 
TGGGAGAAGA ACCAGTCCAT CGCCATCGAG CTCTCGGACC TGGTCGTCTA CTGCAAGCCA 
ACCAGCAAGA CCAAGGACAA CCTCGAAAAC CCTGACTTCC GAGAAATCCG CTCCTTCGTG 
GAGACGAAGG CTGACAGTAT CGTCAGACAG AAGCCCAGCG ATCTCCTGAA ATACAATCAA 
AAGGGCCTGA CCCGTGTGTA CCCCAAAGGA CAGCGGGTGG ACTCGTCCAA CTATGACCCC 
TTCCGCCTCT GGCTGTGCGG CTCCCAGATG GTGGCACTCA ATTTCCAGAC TCCAGATAAG 
TACATGCAGA TGAACCACGC CCTGTTCTCC CTCAACGGGC GGACGGGCTA CGTCCTGCAG 
CCTGAGAGCA TGAGGGCGGA GAAGTACGAC CCCATGCCAC CCGAGTCCCA GAGGAAGATC 
CAGATGACCC TGACGGTCAA GGTGCTGGGG GCCCGTCACC TCCCCAAGCC CGGCCGCAGC 
ATCGCGTGCC CCTTCGTGGA GGTGGAGATA TGCGGAGCGG AGTACGACAG CAACAAGTTT 
AAGACGACGG TTGTGAATGA CAATGGCCTC AGCCCTGTTT GGACCCCAAC GCAGGAGAAG 
GTGACGTTTG AGATTTATGA CCCCAACCTT GCATTTCTGC GCTTTGTGGT TTATGAGGAA 
GATATGTTCA GCGATCCCAA CTTTCTGGCC CATGCCACGT ACCCCATTAA AGGAATCAAG 
TCAGGATTTA GATCTGTTCC TCTGAAGAAC GGCTACAGTG AAGACATTGA GCTGGCGTCC 
CTCCTGGTTT TCTGTGAGAT GCGGCCAGTC CTGGAGAGCG AAGAGGAGCT GTATTCCTCC 
TGCCGCCAGC TGCGGAGGCG GCAAGAAGAA CTGAACAACC AGCTCTTTCT GTACGACACC 
CACCAGAACC TGCGCAGCGC CACCCGCGAT GCCCTGGTCA GAGAATTCAA CGTCAACGAG 
AACCAGCTGC AGCTGTACCA GGAGAAGTGC AACCGCAGGT TAAGAGAGAA GAGAGTCAGC 
AACAGCAAGT TTTACTCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010812684.1
CDS166..3966
Translation

Target ORF information:

RefSeq Version XM_010814382.2
Organism Bos taurus(cattle)
Definition Bos taurus phospholipase C gamma 2 (PLCG2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010814382.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCGAGCA CCATGGCCAA CGTGGACACC CTCCCGGAGT ATGAGAAGAG TCAGATCAAG 
CGGGCCCTGG AGCTGGGCAC CGTCATGACC GTGTTCAGCT TCCGCAAGTC CACCCCGGAG 
CGGAGGACCG TCCAGGTGAT CATGGAGACG CGGCAGGTGG CCTGGAGCAA GACGGCCGAC 
AAGATCGAGG GCTTCCTGGA CATCATGGAG ATAAAGGAGA TCCGGCCGGG GAAGAGCTCC 
AAGGATTTCG AGCGCGCGAA AGCGGTCCGC CAGAAGGAAG AATGCTGCTT CACCGTCCTG 
TATGGCTCCC AGTTTATCCT CAGCACGCTC AGCCTGGCAG CCGACTCGAA GGAGGATGCC 
GCTAAGTGGC TGTCTGGCCT GAAGATCTTA CACCAGGAAG TGATGAGCGC GTCCACCCCC 
ACCATCATCG AGAGCTGGCT GAGAAAGCAG ATTTATTCTG TGGATCAAAC CAGGAGAAAC 
AGCATCAGCC TCCGGGAATT GAAGACCATC TTGCCCCTGG TCAACTTCAA AGTGAGCAGT 
GCCAAGTTCC TCAAAGATAA GTTCCTGGAA ATAGGCGCAC ACAAAGATGA ACTCAGCTTT 
GAACAGTTCC ATCTCTTCTA TAAAAAACTT ATGTTTGAAC AGCAAAAATC GATCCTTGAC 
GAATTCAAAA AGGATTCTTC CGTGTTCCTC TTGGGCAACA CCGACCGGCC AGACGCCTCT 
GCTGTCCACC TGCACGACTT TCAGCGGTTT CTCTTGCATG AACAGCAGGA GCTTTGGGCT 
CAGGATCTGA ACAAAGTCCG TGAGCGGATG ACAAAGTTTA TTGACGACAC CATGAGGGAA 
ACTGCTGAAC CCTTCCTGTT TGTGGATGAG TTCCTCACGT ACCTGTTTTC CCGGGAGAAC 
AGCATCTGGG ACGAGAAGTA CGACGTGGTG GACATGCAGG ACATGAACAA CCCCTTGTCT 
CATTACTGGA TCTCCTCGTC GCACAACACG TACCTCACGG GGGACCAGCT GCGGAGTGAG 
TCGTCCACGG AGGCCTACAT CCGCTGCCTG CGGATGGGCT GCCGCTGCAT CGAGCTGGAC 
TGCTGGGACG GGCCCGATGG CAAGCCCATC ATCTACCACG GCTGGACGCG GACCACCAAG 
ATCAAGTTCG ACGACGTTGT GCAGGCCATC AAAGACCACG CCTTTGTCAC CTCCAGCTTC 
CCCGTGATCC TGTCCATCGA GGAGCACTGC TGTGTGGAGC AGCAGCGCCA CATGGCCAGG 
GTGTTCAAGG AAGTGTTTGG AGACCTGCTG CTGACCAAAC CCAAGGAGGC CAGTGCCGAC 
CAGCTGCCGT CGCCCAGCCA GCTGCGCGGG AAGATCATCA TCAAGCATAA GAAGCTGGGC 
CCCCGCGGTG ACGTGGACGT CAACATGGAG GACAAGAAGG ACGAGCACAA GCAGCAGGGC 
GAACTGCACA TGTGGGACCC CATCGACCAG AAATGGACTC GGCACTACTG TGCTGTTGCT 
GATGCCAAGC TGTCCTTCAG CGATGACATT GAACAGACTG TGGAAGAGGA GCTGCCCCTG 
GATATACCCC CAACAGAGCT GCATTTTGGG GAGAAGTGGT TCCACAAGAA GGTGGAGAAG 
AGGACAAGTG CCGAGAAGCT GCTACAGGAA TACTGCGCCG AGACAGGGGG CAAGGATGGG 
ACCTTCTTGG TGCGGGAGAG TGAGACCTTC CCCAACGACT ACACCTTGTC CTTCTGGCGG 
TCGGGCCGCG TGCAGCACTG CCGGATCCGC TCCACCATGG AGGGTGGGAC CATGAAGTAC 
TACCTGACCG ACAACCTCAT GTTCACCAGC ATCTACGCCC TCATCCAGCA CTACCGAGAG 
ACACACCTGC GCTGTGCCGA GTTCGAGCTC CGGCTGACCG ACCCCGTGCC CAACCCCAAC 
CCGCACGAGT CCAAGCCGTG GTACTACGAT GGGCTGAGCC GTGGAGAAGC GGAGGACATG 
CTGATGCGGA TCCCCCGGGA CGGGGCCTTC CTGATTCGGA AGCGGGAAGG TACCGACTCC 
TACGCCATCA CCTTCAGGGC CAGGGGCAAG GTGAAGCACT GTCGCATCAA CCGAGACGGC 
CGGCACTTCG TGCTGGGCAC CTCCGCCTAC TTCGAGAGCC TCGTGGAGCT GGTCAGTTAC 
TACGAGAAGC ACGCGCTCTA CCGGAAGATG AAGCTGCGCT ACCCCGTGAC CCCCGAGCTC 
CTGGAGCGCT ACAACACGGA AAGAGATATA AACTCCCTCT ACGATGTCAG CAGGATGTAT 
GTGGATCCCA GTGAAATCAA TCCTTCAATG CCTCAGAGAA CCGTGAAAGC TCTGTATGAC 
TACAGGGCCA AGCAGAGTGA CGAGCTGAGC TTTTGCCGCG GTGCCCTCAT CCACAACGTC 
TCCAAGGAGC CTGGGGGCTG GTGGAAAGGA GACTATGGGA CCAGAATCCA GCAGTACTTC 
CCGTCCAATT ACGTCGAGGA CATTTCACCA ACAGAAGGGG AGGAGGTGGA TAAGCAGATT 
ATTGAAGACA ATCCCTTAGG GTCCCTTTGC AGAGGAATCC TGAATCTCAA CATAAGGAAT 
GTTGTAAAAG CCCCACATGG GAAGAACCAG AAGCCTTTCG TCTTTATCTT GGAGCCCAAG 
AAGCAGGGCG ACCCTCCGGT AGAGTTTGCC ACGGACAGCG TGGAGGAGCT GTTTGAGTGG 
TTCCAGAGCA TCCGGGAGAT CACCTGGAAG ATGGACGCCA AGGAGAACAA CATGAAGTAC 
TGGGAGAAGA ACCAGTCCAT CGCCATCGAG CTCTCGGACC TGGTCGTCTA CTGCAAGCCA 
ACCAGCAAGA CCAAGGACAA CCTCGAAAAC CCTGACTTCC GAGAAATCCG CTCCTTCGTG 
GAGACGAAGG CTGACAGTAT CGTCAGACAG AAGCCCAGCG ATCTCCTGAA ATACAATCAA 
AAGGGCCTGA CCCGTGTGTA CCCCAAAGGA CAGCGGGTGG ACTCGTCCAA CTATGACCCC 
TTCCGCCTCT GGCTGTGCGG CTCCCAGATG GTGGCACTCA ATTTCCAGAC TCCAGATAAG 
TACATGCAGA TGAACCACGC CCTGTTCTCC CTCAACGGGC GGACGGGCTA CGTCCTGCAG 
CCTGAGAGCA TGAGGGCGGA GAAGTACGAC CCCATGCCAC CCGAGTCCCA GAGGAAGATC 
CAGATGACCC TGACGGTCAA GGTGCTGGGG GCCCGTCACC TCCCCAAGCC CGGCCGCAGC 
ATCGCGTGCC CCTTCGTGGA GGTGGAGATA TGCGGAGCGG AGTACGACAG CAACAAGTTT 
AAGACGACGG TTGTGAATGA CAATGGCCTC AGCCCTGTTT GGACCCCAAC GCAGGAGAAG 
GTGACGTTTG AGATTTATGA CCCCAACCTT GCATTTCTGC GCTTTGTGGT TTATGAGGAA 
GATATGTTCA GCGATCCCAA CTTTCTGGCC CATGCCACGT ACCCCATTAA AGGAATCAAG 
TCAGGATTTA GATCTGTTCC TCTGAAGAAC GGCTACAGTG AAGACATTGA GCTGGCGTCC 
CTCCTGGTTT TCTGTGAGAT GCGGCCAGTC CTGGAGAGCG AAGAGGAGCT GTATTCCTCC 
TGCCGCCAGC TGCGGAGGCG GCAAGAAGAA CTGAACAACC AGCTCTTTCT GTACGACACC 
CACCAGAACC TGCGCAGCGC CACCCGCGAT GCCCTGGTCA GAGAATTCAA CGTCAACGAG 
AACCAGCTGC AGCTGTACCA GGAGAAGTGC AACCGCAGGT TAAGAGAGAA GAGAGTCAGC 
AACAGCAAGT TTTACTCATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed