LRP6 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following LRP6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRP6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa19703 XM_005207028.4
Latest version!
Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19703 XM_015463446.1
Latest version!
Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19704 XM_002687783.6
Latest version!
Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19704 XM_015463447.1
Latest version!
Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19705 XM_015463448.1
Latest version!
Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19705 XM_005207029.4
Latest version!
Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19706 XM_015463449.1
Latest version!
Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19706 XM_003586111.5
Latest version!
Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OBa19705 XM_005207029.3 Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19703 XM_005207028.3 Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19704 XM_002687783.5 Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa19706 XM_003586111.4 Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa19703
    Clone ID Related Accession (Same CDS sequence) XM_005207028.3 , XM_015463446.1 , XM_005207028.4
    Accession Version XM_005207028.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103936.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAACCCCCAA CATGTTCTCC TCAGCAGTTT ACTTGTTTCA CGGGAGAAAT TGACTGTATC
    CCTGTGGCTT GGCGGTGTGA TGGGTTTACT GAATGTGAAG ACCACAGTGA TGAACTCAAT
    TGTCCTGTGT GCTCAGAGTC CCAGTTCCAG TGCGCCAGTG GACAGTGTAT CGATGGTGCC
    CTGCGATGCA ACGGAGACGC GAACTGCCAG GATAAGTCAG ATGAGAAGAA CTGTGAAGTA
    CTCTGTTTAA TTGATCAGTT CCGCTGTGCC AATGGTCAAT GCATTGGAAA ACACAAGAAA
    TGTGATCATA ATTTGGATTG CAGTGACAAA TCAGATGAAC TGGATTGTTA TCCAACTGAG
    GAGCCAGCAC CACAGGCCAC CAATACAGTG GGTTCTGTTA TTGGAGTAAT TGTTACCATC
    TTTGTGTCTG GAACCATATA CTTTATCTGC CAGAGGATGT TGTGTCCACG GATGAAGGGC
    GATGGGGAAA CCATGACTAA CGACTATGTA GTTCATGGAC CAGCTTCTGT GCCCCTTGGT
    TACGTGCCAC ACCCCAGTTC TTTGTCAGGA TCTCTTCCAG GAATGTCTCG TGGTAAATCA
    ATGATCAGTT CCCTCAGTAT CATGGGGGGG AGCAGTGGAC CCCCTTATGA CCGAGCCCAT
    GTCACAGGAG CATCGTCAAG TAGTTCATCA AGCACCAAAG GCACTTACTT CCCTGCAATT
    TTGAACCCTC CGCCATCCCC GGCCACAGAG CGATCACATT ACACCATGGA ATTTGGTTAT
    TCTTCTAACA GCCCTTCCAC TCACAGGTCC TACAGCTACA GGCCGTACAG CTACCGGCAC
    TTTGCGCCCC CCACCACGCC CTGCAGCACA GATGTGTGTG ACAGTGACTA TGCTCCTAGC
    CGAAGGATGA CCTCAGTGGT GACGGCCAAG GGCTACACTA GTGACTTGAA CTATGACTCA
    GAGCCTGTGC CCCCACCTCC CACGCCCCGA AGCCAGTACT TGTCAGCAGA AGAGAACTAT
    GAAAGCTGCC CGCCTTCTCC ATACACGGAG AGGAGCTACT CTCATCACCT CTACCCACCG
    CCACCCTCTC CCTGTACAGA CTCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207085.1
    CDS126..5075
    Translation

    Target ORF information:

    RefSeq Version XM_005207028.3
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207028.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAACCCCCAA CATGTTCTCC TCAGCAGTTT ACTTGTTTCA CGGGAGAAAT TGACTGTATC
    CCTGTGGCTT GGCGGTGTGA TGGGTTTACT GAATGTGAAG ACCACAGTGA TGAACTCAAT
    TGTCCTGTGT GCTCAGAGTC CCAGTTCCAG TGCGCCAGTG GACAGTGTAT CGATGGTGCC
    CTGCGATGCA ACGGAGACGC GAACTGCCAG GATAAGTCAG ATGAGAAGAA CTGTGAAGTA
    CTCTGTTTAA TTGATCAGTT CCGCTGTGCC AATGGTCAAT GCATTGGAAA ACACAAGAAA
    TGTGATCATA ATTTGGATTG CAGTGACAAA TCAGATGAAC TGGATTGTTA TCCAACTGAG
    GAGCCAGCAC CACAGGCCAC CAATACAGTG GGTTCTGTTA TTGGAGTAAT TGTTACCATC
    TTTGTGTCTG GAACCATATA CTTTATCTGC CAGAGGATGT TGTGTCCACG GATGAAGGGC
    GATGGGGAAA CCATGACTAA CGACTATGTA GTTCATGGAC CAGCTTCTGT GCCCCTTGGT
    TACGTGCCAC ACCCCAGTTC TTTGTCAGGA TCTCTTCCAG GAATGTCTCG TGGTAAATCA
    ATGATCAGTT CCCTCAGTAT CATGGGGGGG AGCAGTGGAC CCCCTTATGA CCGAGCCCAT
    GTCACAGGAG CATCGTCAAG TAGTTCATCA AGCACCAAAG GCACTTACTT CCCTGCAATT
    TTGAACCCTC CGCCATCCCC GGCCACAGAG CGATCACATT ACACCATGGA ATTTGGTTAT
    TCTTCTAACA GCCCTTCCAC TCACAGGTCC TACAGCTACA GGCCGTACAG CTACCGGCAC
    TTTGCGCCCC CCACCACGCC CTGCAGCACA GATGTGTGTG ACAGTGACTA TGCTCCTAGC
    CGAAGGATGA CCTCAGTGGT GACGGCCAAG GGCTACACTA GTGACTTGAA CTATGACTCA
    GAGCCTGTGC CCCCACCTCC CACGCCCCGA AGCCAGTACT TGTCAGCAGA AGAGAACTAT
    GAAAGCTGCC CGCCTTCTCC ATACACGGAG AGGAGCTACT CTCATCACCT CTACCCACCG
    CCACCCTCTC CCTGTACAGA CTCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19703
    Clone ID Related Accession (Same CDS sequence) XM_005207028.3 , XM_015463446.1 , XM_005207028.4
    Accession Version XM_015463446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644840.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAACCCCCAA CATGTTCTCC TCAGCAGTTT ACTTGTTTCA CGGGAGAAAT TGACTGTATC
    CCTGTGGCTT GGCGGTGTGA TGGGTTTACT GAATGTGAAG ACCACAGTGA TGAACTCAAT
    TGTCCTGTGT GCTCAGAGTC CCAGTTCCAG TGCGCCAGTG GACAGTGTAT CGATGGTGCC
    CTGCGATGCA ACGGAGACGC GAACTGCCAG GATAAGTCAG ATGAGAAGAA CTGTGAAGTA
    CTCTGTTTAA TTGATCAGTT CCGCTGTGCC AATGGTCAAT GCATTGGAAA ACACAAGAAA
    TGTGATCATA ATTTGGATTG CAGTGACAAA TCAGATGAAC TGGATTGTTA TCCAACTGAG
    GAGCCAGCAC CACAGGCCAC CAATACAGTG GGTTCTGTTA TTGGAGTAAT TGTTACCATC
    TTTGTGTCTG GAACCATATA CTTTATCTGC CAGAGGATGT TGTGTCCACG GATGAAGGGC
    GATGGGGAAA CCATGACTAA CGACTATGTA GTTCATGGAC CAGCTTCTGT GCCCCTTGGT
    TACGTGCCAC ACCCCAGTTC TTTGTCAGGA TCTCTTCCAG GAATGTCTCG TGGTAAATCA
    ATGATCAGTT CCCTCAGTAT CATGGGGGGG AGCAGTGGAC CCCCTTATGA CCGAGCCCAT
    GTCACAGGAG CATCGTCAAG TAGTTCATCA AGCACCAAAG GCACTTACTT CCCTGCAATT
    TTGAACCCTC CGCCATCCCC GGCCACAGAG CGATCACATT ACACCATGGA ATTTGGTTAT
    TCTTCTAACA GCCCTTCCAC TCACAGGTCC TACAGCTACA GGCCGTACAG CTACCGGCAC
    TTTGCGCCCC CCACCACGCC CTGCAGCACA GATGTGTGTG ACAGTGACTA TGCTCCTAGC
    CGAAGGATGA CCTCAGTGGT GACGGCCAAG GGCTACACTA GTGACTTGAA CTATGACTCA
    GAGCCTGTGC CCCCACCTCC CACGCCCCGA AGCCAGTACT TGTCAGCAGA AGAGAACTAT
    GAAAGCTGCC CGCCTTCTCC ATACACGGAG AGGAGCTACT CTCATCACCT CTACCCACCG
    CCACCCTCTC CCTGTACAGA CTCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318932.1
    CDS126..5075
    Translation

    Target ORF information:

    RefSeq Version XM_015463446.1
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAACCCCCAA CATGTTCTCC TCAGCAGTTT ACTTGTTTCA CGGGAGAAAT TGACTGTATC
    CCTGTGGCTT GGCGGTGTGA TGGGTTTACT GAATGTGAAG ACCACAGTGA TGAACTCAAT
    TGTCCTGTGT GCTCAGAGTC CCAGTTCCAG TGCGCCAGTG GACAGTGTAT CGATGGTGCC
    CTGCGATGCA ACGGAGACGC GAACTGCCAG GATAAGTCAG ATGAGAAGAA CTGTGAAGTA
    CTCTGTTTAA TTGATCAGTT CCGCTGTGCC AATGGTCAAT GCATTGGAAA ACACAAGAAA
    TGTGATCATA ATTTGGATTG CAGTGACAAA TCAGATGAAC TGGATTGTTA TCCAACTGAG
    GAGCCAGCAC CACAGGCCAC CAATACAGTG GGTTCTGTTA TTGGAGTAAT TGTTACCATC
    TTTGTGTCTG GAACCATATA CTTTATCTGC CAGAGGATGT TGTGTCCACG GATGAAGGGC
    GATGGGGAAA CCATGACTAA CGACTATGTA GTTCATGGAC CAGCTTCTGT GCCCCTTGGT
    TACGTGCCAC ACCCCAGTTC TTTGTCAGGA TCTCTTCCAG GAATGTCTCG TGGTAAATCA
    ATGATCAGTT CCCTCAGTAT CATGGGGGGG AGCAGTGGAC CCCCTTATGA CCGAGCCCAT
    GTCACAGGAG CATCGTCAAG TAGTTCATCA AGCACCAAAG GCACTTACTT CCCTGCAATT
    TTGAACCCTC CGCCATCCCC GGCCACAGAG CGATCACATT ACACCATGGA ATTTGGTTAT
    TCTTCTAACA GCCCTTCCAC TCACAGGTCC TACAGCTACA GGCCGTACAG CTACCGGCAC
    TTTGCGCCCC CCACCACGCC CTGCAGCACA GATGTGTGTG ACAGTGACTA TGCTCCTAGC
    CGAAGGATGA CCTCAGTGGT GACGGCCAAG GGCTACACTA GTGACTTGAA CTATGACTCA
    GAGCCTGTGC CCCCACCTCC CACGCCCCGA AGCCAGTACT TGTCAGCAGA AGAGAACTAT
    GAAAGCTGCC CGCCTTCTCC ATACACGGAG AGGAGCTACT CTCATCACCT CTACCCACCG
    CCACCCTCTC CCTGTACAGA CTCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19703
    Clone ID Related Accession (Same CDS sequence) XM_005207028.3 , XM_015463446.1 , XM_005207028.4
    Accession Version XM_005207028.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207028.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAACCCCCAA CATGTTCTCC TCAGCAGTTT ACTTGTTTCA CGGGAGAAAT TGACTGTATC
    CCTGTGGCTT GGCGGTGTGA TGGGTTTACT GAATGTGAAG ACCACAGTGA TGAACTCAAT
    TGTCCTGTGT GCTCAGAGTC CCAGTTCCAG TGCGCCAGTG GACAGTGTAT CGATGGTGCC
    CTGCGATGCA ACGGAGACGC GAACTGCCAG GATAAGTCAG ATGAGAAGAA CTGTGAAGTA
    CTCTGTTTAA TTGATCAGTT CCGCTGTGCC AATGGTCAAT GCATTGGAAA ACACAAGAAA
    TGTGATCATA ATTTGGATTG CAGTGACAAA TCAGATGAAC TGGATTGTTA TCCAACTGAG
    GAGCCAGCAC CACAGGCCAC CAATACAGTG GGTTCTGTTA TTGGAGTAAT TGTTACCATC
    TTTGTGTCTG GAACCATATA CTTTATCTGC CAGAGGATGT TGTGTCCACG GATGAAGGGC
    GATGGGGAAA CCATGACTAA CGACTATGTA GTTCATGGAC CAGCTTCTGT GCCCCTTGGT
    TACGTGCCAC ACCCCAGTTC TTTGTCAGGA TCTCTTCCAG GAATGTCTCG TGGTAAATCA
    ATGATCAGTT CCCTCAGTAT CATGGGGGGG AGCAGTGGAC CCCCTTATGA CCGAGCCCAT
    GTCACAGGAG CATCGTCAAG TAGTTCATCA AGCACCAAAG GCACTTACTT CCCTGCAATT
    TTGAACCCTC CGCCATCCCC GGCCACAGAG CGATCACATT ACACCATGGA ATTTGGTTAT
    TCTTCTAACA GCCCTTCCAC TCACAGGTCC TACAGCTACA GGCCGTACAG CTACCGGCAC
    TTTGCGCCCC CCACCACGCC CTGCAGCACA GATGTGTGTG ACAGTGACTA TGCTCCTAGC
    CGAAGGATGA CCTCAGTGGT GACGGCCAAG GGCTACACTA GTGACTTGAA CTATGACTCA
    GAGCCTGTGC CCCCACCTCC CACGCCCCGA AGCCAGTACT TGTCAGCAGA AGAGAACTAT
    GAAAGCTGCC CGCCTTCTCC ATACACGGAG AGGAGCTACT CTCATCACCT CTACCCACCG
    CCACCCTCTC CCTGTACAGA CTCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207085.1
    CDS141..5090
    Translation

    Target ORF information:

    RefSeq Version XM_005207028.4
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207028.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAACCCCCAA CATGTTCTCC TCAGCAGTTT ACTTGTTTCA CGGGAGAAAT TGACTGTATC
    CCTGTGGCTT GGCGGTGTGA TGGGTTTACT GAATGTGAAG ACCACAGTGA TGAACTCAAT
    TGTCCTGTGT GCTCAGAGTC CCAGTTCCAG TGCGCCAGTG GACAGTGTAT CGATGGTGCC
    CTGCGATGCA ACGGAGACGC GAACTGCCAG GATAAGTCAG ATGAGAAGAA CTGTGAAGTA
    CTCTGTTTAA TTGATCAGTT CCGCTGTGCC AATGGTCAAT GCATTGGAAA ACACAAGAAA
    TGTGATCATA ATTTGGATTG CAGTGACAAA TCAGATGAAC TGGATTGTTA TCCAACTGAG
    GAGCCAGCAC CACAGGCCAC CAATACAGTG GGTTCTGTTA TTGGAGTAAT TGTTACCATC
    TTTGTGTCTG GAACCATATA CTTTATCTGC CAGAGGATGT TGTGTCCACG GATGAAGGGC
    GATGGGGAAA CCATGACTAA CGACTATGTA GTTCATGGAC CAGCTTCTGT GCCCCTTGGT
    TACGTGCCAC ACCCCAGTTC TTTGTCAGGA TCTCTTCCAG GAATGTCTCG TGGTAAATCA
    ATGATCAGTT CCCTCAGTAT CATGGGGGGG AGCAGTGGAC CCCCTTATGA CCGAGCCCAT
    GTCACAGGAG CATCGTCAAG TAGTTCATCA AGCACCAAAG GCACTTACTT CCCTGCAATT
    TTGAACCCTC CGCCATCCCC GGCCACAGAG CGATCACATT ACACCATGGA ATTTGGTTAT
    TCTTCTAACA GCCCTTCCAC TCACAGGTCC TACAGCTACA GGCCGTACAG CTACCGGCAC
    TTTGCGCCCC CCACCACGCC CTGCAGCACA GATGTGTGTG ACAGTGACTA TGCTCCTAGC
    CGAAGGATGA CCTCAGTGGT GACGGCCAAG GGCTACACTA GTGACTTGAA CTATGACTCA
    GAGCCTGTGC CCCCACCTCC CACGCCCCGA AGCCAGTACT TGTCAGCAGA AGAGAACTAT
    GAAAGCTGCC CGCCTTCTCC ATACACGGAG AGGAGCTACT CTCATCACCT CTACCCACCG
    CCACCCTCTC CCTGTACAGA CTCCTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19704
    Clone ID Related Accession (Same CDS sequence) XM_015463447.1 , XM_002687783.6 , XM_002687783.5
    Accession Version XM_015463447.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644840.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAACCCCC AACATGTTCT CCTCAGCAGT TTACTTGTTT CACGGGAGAA
    ATTGACTGTA TCCCTGTGGC TTGGCGGTGT GATGGGTTTA CTGAATGTGA AGACCACAGT
    GATGAACTCA ATTGTCCTGT GTGCTCAGAG TCCCAGTTCC AGTGCGCCAG TGGACAGTGT
    ATCGATGGTG CCCTGCGATG CAACGGAGAC GCGAACTGCC AGGATAAGTC AGATGAGAAG
    AACTGTGAAG TACTCTGTTT AATTGATCAG TTCCGCTGTG CCAATGGTCA ATGCATTGGA
    AAACACAAGA AATGTGATCA TAATTTGGAT TGCAGTGACA AATCAGATGA ACTGGATTGT
    TATCCAACTG AGGAGCCAGC ACCACAGGCC ACCAATACAG TGGGTTCTGT TATTGGAGTA
    ATTGTTACCA TCTTTGTGTC TGGAACCATA TACTTTATCT GCCAGAGGAT GTTGTGTCCA
    CGGATGAAGG GCGATGGGGA AACCATGACT AACGACTATG TAGTTCATGG ACCAGCTTCT
    GTGCCCCTTG GTTACGTGCC ACACCCCAGT TCTTTGTCAG GATCTCTTCC AGGAATGTCT
    CGTGGTAAAT CAATGATCAG TTCCCTCAGT ATCATGGGGG GGAGCAGTGG ACCCCCTTAT
    GACCGAGCCC ATGTCACAGG AGCATCGTCA AGTAGTTCAT CAAGCACCAA AGGCACTTAC
    TTCCCTGCAA TTTTGAACCC TCCGCCATCC CCGGCCACAG AGCGATCACA TTACACCATG
    GAATTTGGTT ATTCTTCTAA CAGCCCTTCC ACTCACAGGT CCTACAGCTA CAGGCCGTAC
    AGCTACCGGC ACTTTGCGCC CCCCACCACG CCCTGCAGCA CAGATGTGTG TGACAGTGAC
    TATGCTCCTA GCCGAAGGAT GACCTCAGTG GTGACGGCCA AGGGCTACAC TAGTGACTTG
    AACTATGACT CAGAGCCTGT GCCCCCACCT CCCACGCCCC GAAGCCAGTA CTTGTCAGCA
    GAAGAGAACT ATGAAAGCTG CCCGCCTTCT CCATACACGG AGAGGAGCTA CTCTCATCAC
    CTCTACCCAC CGCCACCCTC TCCCTGTACA GACTCCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318933.1
    CDS125..4966
    Translation

    Target ORF information:

    RefSeq Version XM_015463447.1
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAACCCCC AACATGTTCT CCTCAGCAGT TTACTTGTTT CACGGGAGAA
    ATTGACTGTA TCCCTGTGGC TTGGCGGTGT GATGGGTTTA CTGAATGTGA AGACCACAGT
    GATGAACTCA ATTGTCCTGT GTGCTCAGAG TCCCAGTTCC AGTGCGCCAG TGGACAGTGT
    ATCGATGGTG CCCTGCGATG CAACGGAGAC GCGAACTGCC AGGATAAGTC AGATGAGAAG
    AACTGTGAAG TACTCTGTTT AATTGATCAG TTCCGCTGTG CCAATGGTCA ATGCATTGGA
    AAACACAAGA AATGTGATCA TAATTTGGAT TGCAGTGACA AATCAGATGA ACTGGATTGT
    TATCCAACTG AGGAGCCAGC ACCACAGGCC ACCAATACAG TGGGTTCTGT TATTGGAGTA
    ATTGTTACCA TCTTTGTGTC TGGAACCATA TACTTTATCT GCCAGAGGAT GTTGTGTCCA
    CGGATGAAGG GCGATGGGGA AACCATGACT AACGACTATG TAGTTCATGG ACCAGCTTCT
    GTGCCCCTTG GTTACGTGCC ACACCCCAGT TCTTTGTCAG GATCTCTTCC AGGAATGTCT
    CGTGGTAAAT CAATGATCAG TTCCCTCAGT ATCATGGGGG GGAGCAGTGG ACCCCCTTAT
    GACCGAGCCC ATGTCACAGG AGCATCGTCA AGTAGTTCAT CAAGCACCAA AGGCACTTAC
    TTCCCTGCAA TTTTGAACCC TCCGCCATCC CCGGCCACAG AGCGATCACA TTACACCATG
    GAATTTGGTT ATTCTTCTAA CAGCCCTTCC ACTCACAGGT CCTACAGCTA CAGGCCGTAC
    AGCTACCGGC ACTTTGCGCC CCCCACCACG CCCTGCAGCA CAGATGTGTG TGACAGTGAC
    TATGCTCCTA GCCGAAGGAT GACCTCAGTG GTGACGGCCA AGGGCTACAC TAGTGACTTG
    AACTATGACT CAGAGCCTGT GCCCCCACCT CCCACGCCCC GAAGCCAGTA CTTGTCAGCA
    GAAGAGAACT ATGAAAGCTG CCCGCCTTCT CCATACACGG AGAGGAGCTA CTCTCATCAC
    CTCTACCCAC CGCCACCCTC TCCCTGTACA GACTCCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19704
    Clone ID Related Accession (Same CDS sequence) XM_015463447.1 , XM_002687783.6 , XM_002687783.5
    Accession Version XM_002687783.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002687783.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAACCCCC AACATGTTCT CCTCAGCAGT TTACTTGTTT CACGGGAGAA
    ATTGACTGTA TCCCTGTGGC TTGGCGGTGT GATGGGTTTA CTGAATGTGA AGACCACAGT
    GATGAACTCA ATTGTCCTGT GTGCTCAGAG TCCCAGTTCC AGTGCGCCAG TGGACAGTGT
    ATCGATGGTG CCCTGCGATG CAACGGAGAC GCGAACTGCC AGGATAAGTC AGATGAGAAG
    AACTGTGAAG TACTCTGTTT AATTGATCAG TTCCGCTGTG CCAATGGTCA ATGCATTGGA
    AAACACAAGA AATGTGATCA TAATTTGGAT TGCAGTGACA AATCAGATGA ACTGGATTGT
    TATCCAACTG AGGAGCCAGC ACCACAGGCC ACCAATACAG TGGGTTCTGT TATTGGAGTA
    ATTGTTACCA TCTTTGTGTC TGGAACCATA TACTTTATCT GCCAGAGGAT GTTGTGTCCA
    CGGATGAAGG GCGATGGGGA AACCATGACT AACGACTATG TAGTTCATGG ACCAGCTTCT
    GTGCCCCTTG GTTACGTGCC ACACCCCAGT TCTTTGTCAG GATCTCTTCC AGGAATGTCT
    CGTGGTAAAT CAATGATCAG TTCCCTCAGT ATCATGGGGG GGAGCAGTGG ACCCCCTTAT
    GACCGAGCCC ATGTCACAGG AGCATCGTCA AGTAGTTCAT CAAGCACCAA AGGCACTTAC
    TTCCCTGCAA TTTTGAACCC TCCGCCATCC CCGGCCACAG AGCGATCACA TTACACCATG
    GAATTTGGTT ATTCTTCTAA CAGCCCTTCC ACTCACAGGT CCTACAGCTA CAGGCCGTAC
    AGCTACCGGC ACTTTGCGCC CCCCACCACG CCCTGCAGCA CAGATGTGTG TGACAGTGAC
    TATGCTCCTA GCCGAAGGAT GACCTCAGTG GTGACGGCCA AGGGCTACAC TAGTGACTTG
    AACTATGACT CAGAGCCTGT GCCCCCACCT CCCACGCCCC GAAGCCAGTA CTTGTCAGCA
    GAAGAGAACT ATGAAAGCTG CCCGCCTTCT CCATACACGG AGAGGAGCTA CTCTCATCAC
    CTCTACCCAC CGCCACCCTC TCCCTGTACA GACTCCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002687829.2
    CDS253..5094
    Translation

    Target ORF information:

    RefSeq Version XM_002687783.6
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002687783.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAACCCCC AACATGTTCT CCTCAGCAGT TTACTTGTTT CACGGGAGAA
    ATTGACTGTA TCCCTGTGGC TTGGCGGTGT GATGGGTTTA CTGAATGTGA AGACCACAGT
    GATGAACTCA ATTGTCCTGT GTGCTCAGAG TCCCAGTTCC AGTGCGCCAG TGGACAGTGT
    ATCGATGGTG CCCTGCGATG CAACGGAGAC GCGAACTGCC AGGATAAGTC AGATGAGAAG
    AACTGTGAAG TACTCTGTTT AATTGATCAG TTCCGCTGTG CCAATGGTCA ATGCATTGGA
    AAACACAAGA AATGTGATCA TAATTTGGAT TGCAGTGACA AATCAGATGA ACTGGATTGT
    TATCCAACTG AGGAGCCAGC ACCACAGGCC ACCAATACAG TGGGTTCTGT TATTGGAGTA
    ATTGTTACCA TCTTTGTGTC TGGAACCATA TACTTTATCT GCCAGAGGAT GTTGTGTCCA
    CGGATGAAGG GCGATGGGGA AACCATGACT AACGACTATG TAGTTCATGG ACCAGCTTCT
    GTGCCCCTTG GTTACGTGCC ACACCCCAGT TCTTTGTCAG GATCTCTTCC AGGAATGTCT
    CGTGGTAAAT CAATGATCAG TTCCCTCAGT ATCATGGGGG GGAGCAGTGG ACCCCCTTAT
    GACCGAGCCC ATGTCACAGG AGCATCGTCA AGTAGTTCAT CAAGCACCAA AGGCACTTAC
    TTCCCTGCAA TTTTGAACCC TCCGCCATCC CCGGCCACAG AGCGATCACA TTACACCATG
    GAATTTGGTT ATTCTTCTAA CAGCCCTTCC ACTCACAGGT CCTACAGCTA CAGGCCGTAC
    AGCTACCGGC ACTTTGCGCC CCCCACCACG CCCTGCAGCA CAGATGTGTG TGACAGTGAC
    TATGCTCCTA GCCGAAGGAT GACCTCAGTG GTGACGGCCA AGGGCTACAC TAGTGACTTG
    AACTATGACT CAGAGCCTGT GCCCCCACCT CCCACGCCCC GAAGCCAGTA CTTGTCAGCA
    GAAGAGAACT ATGAAAGCTG CCCGCCTTCT CCATACACGG AGAGGAGCTA CTCTCATCAC
    CTCTACCCAC CGCCACCCTC TCCCTGTACA GACTCCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19704
    Clone ID Related Accession (Same CDS sequence) XM_015463447.1 , XM_002687783.6 , XM_002687783.5
    Accession Version XM_002687783.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103936.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002687783.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAACCCCC AACATGTTCT CCTCAGCAGT TTACTTGTTT CACGGGAGAA
    ATTGACTGTA TCCCTGTGGC TTGGCGGTGT GATGGGTTTA CTGAATGTGA AGACCACAGT
    GATGAACTCA ATTGTCCTGT GTGCTCAGAG TCCCAGTTCC AGTGCGCCAG TGGACAGTGT
    ATCGATGGTG CCCTGCGATG CAACGGAGAC GCGAACTGCC AGGATAAGTC AGATGAGAAG
    AACTGTGAAG TACTCTGTTT AATTGATCAG TTCCGCTGTG CCAATGGTCA ATGCATTGGA
    AAACACAAGA AATGTGATCA TAATTTGGAT TGCAGTGACA AATCAGATGA ACTGGATTGT
    TATCCAACTG AGGAGCCAGC ACCACAGGCC ACCAATACAG TGGGTTCTGT TATTGGAGTA
    ATTGTTACCA TCTTTGTGTC TGGAACCATA TACTTTATCT GCCAGAGGAT GTTGTGTCCA
    CGGATGAAGG GCGATGGGGA AACCATGACT AACGACTATG TAGTTCATGG ACCAGCTTCT
    GTGCCCCTTG GTTACGTGCC ACACCCCAGT TCTTTGTCAG GATCTCTTCC AGGAATGTCT
    CGTGGTAAAT CAATGATCAG TTCCCTCAGT ATCATGGGGG GGAGCAGTGG ACCCCCTTAT
    GACCGAGCCC ATGTCACAGG AGCATCGTCA AGTAGTTCAT CAAGCACCAA AGGCACTTAC
    TTCCCTGCAA TTTTGAACCC TCCGCCATCC CCGGCCACAG AGCGATCACA TTACACCATG
    GAATTTGGTT ATTCTTCTAA CAGCCCTTCC ACTCACAGGT CCTACAGCTA CAGGCCGTAC
    AGCTACCGGC ACTTTGCGCC CCCCACCACG CCCTGCAGCA CAGATGTGTG TGACAGTGAC
    TATGCTCCTA GCCGAAGGAT GACCTCAGTG GTGACGGCCA AGGGCTACAC TAGTGACTTG
    AACTATGACT CAGAGCCTGT GCCCCCACCT CCCACGCCCC GAAGCCAGTA CTTGTCAGCA
    GAAGAGAACT ATGAAAGCTG CCCGCCTTCT CCATACACGG AGAGGAGCTA CTCTCATCAC
    CTCTACCCAC CGCCACCCTC TCCCTGTACA GACTCCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002687829.2
    CDS125..4966
    Translation

    Target ORF information:

    RefSeq Version XM_002687783.5
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002687783.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAACCCCC AACATGTTCT CCTCAGCAGT TTACTTGTTT CACGGGAGAA
    ATTGACTGTA TCCCTGTGGC TTGGCGGTGT GATGGGTTTA CTGAATGTGA AGACCACAGT
    GATGAACTCA ATTGTCCTGT GTGCTCAGAG TCCCAGTTCC AGTGCGCCAG TGGACAGTGT
    ATCGATGGTG CCCTGCGATG CAACGGAGAC GCGAACTGCC AGGATAAGTC AGATGAGAAG
    AACTGTGAAG TACTCTGTTT AATTGATCAG TTCCGCTGTG CCAATGGTCA ATGCATTGGA
    AAACACAAGA AATGTGATCA TAATTTGGAT TGCAGTGACA AATCAGATGA ACTGGATTGT
    TATCCAACTG AGGAGCCAGC ACCACAGGCC ACCAATACAG TGGGTTCTGT TATTGGAGTA
    ATTGTTACCA TCTTTGTGTC TGGAACCATA TACTTTATCT GCCAGAGGAT GTTGTGTCCA
    CGGATGAAGG GCGATGGGGA AACCATGACT AACGACTATG TAGTTCATGG ACCAGCTTCT
    GTGCCCCTTG GTTACGTGCC ACACCCCAGT TCTTTGTCAG GATCTCTTCC AGGAATGTCT
    CGTGGTAAAT CAATGATCAG TTCCCTCAGT ATCATGGGGG GGAGCAGTGG ACCCCCTTAT
    GACCGAGCCC ATGTCACAGG AGCATCGTCA AGTAGTTCAT CAAGCACCAA AGGCACTTAC
    TTCCCTGCAA TTTTGAACCC TCCGCCATCC CCGGCCACAG AGCGATCACA TTACACCATG
    GAATTTGGTT ATTCTTCTAA CAGCCCTTCC ACTCACAGGT CCTACAGCTA CAGGCCGTAC
    AGCTACCGGC ACTTTGCGCC CCCCACCACG CCCTGCAGCA CAGATGTGTG TGACAGTGAC
    TATGCTCCTA GCCGAAGGAT GACCTCAGTG GTGACGGCCA AGGGCTACAC TAGTGACTTG
    AACTATGACT CAGAGCCTGT GCCCCCACCT CCCACGCCCC GAAGCCAGTA CTTGTCAGCA
    GAAGAGAACT ATGAAAGCTG CCCGCCTTCT CCATACACGG AGAGGAGCTA CTCTCATCAC
    CTCTACCCAC CGCCACCCTC TCCCTGTACA GACTCCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19705
    Clone ID Related Accession (Same CDS sequence) XM_005207029.4 , XM_015463448.1 , XM_005207029.3
    Accession Version XM_005207029.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207029.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAGTCCCAGT TCCAGTGCGC CAGTGGACAG TGTATCGATG GTGCCCTGCG ATGCAACGGA
    GACGCGAACT GCCAGGATAA GTCAGATGAG AAGAACTGTG AAGTACTCTG TTTAATTGAT
    CAGTTCCGCT GTGCCAATGG TCAATGCATT GGAAAACACA AGAAATGTGA TCATAATTTG
    GATTGCAGTG ACAAATCAGA TGAACTGGAT TGTTATCCAA CTGAGGAGCC AGCACCACAG
    GCCACCAATA CAGTGGGTTC TGTTATTGGA GTAATTGTTA CCATCTTTGT GTCTGGAACC
    ATATACTTTA TCTGCCAGAG GATGTTGTGT CCACGGATGA AGGGCGATGG GGAAACCATG
    ACTAACGACT ATGTAGTTCA TGGACCAGCT TCTGTGCCCC TTGGTTACGT GCCACACCCC
    AGTTCTTTGT CAGGATCTCT TCCAGGAATG TCTCGTGGTA AATCAATGAT CAGTTCCCTC
    AGTATCATGG GGGGGAGCAG TGGACCCCCT TATGACCGAG CCCATGTCAC AGGAGCATCG
    TCAAGTAGTT CATCAAGCAC CAAAGGCACT TACTTCCCTG CAATTTTGAA CCCTCCGCCA
    TCCCCGGCCA CAGAGCGATC ACATTACACC ATGGAATTTG GTTATTCTTC TAACAGCCCT
    TCCACTCACA GGTCCTACAG CTACAGGCCG TACAGCTACC GGCACTTTGC GCCCCCCACC
    ACGCCCTGCA GCACAGATGT GTGTGACAGT GACTATGCTC CTAGCCGAAG GATGACCTCA
    GTGGTGACGG CCAAGGGCTA CACTAGTGAC TTGAACTATG ACTCAGAGCC TGTGCCCCCA
    CCTCCCACGC CCCGAAGCCA GTACTTGTCA GCAGAAGAGA ACTATGAAAG CTGCCCGCCT
    TCTCCATACA CGGAGAGGAG CTACTCTCAT CACCTCTACC CACCGCCACC CTCTCCCTGT
    ACAGACTCCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207086.1
    CDS141..4955
    Translation

    Target ORF information:

    RefSeq Version XM_005207029.4
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207029.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAGTCCCAGT TCCAGTGCGC CAGTGGACAG TGTATCGATG GTGCCCTGCG ATGCAACGGA
    GACGCGAACT GCCAGGATAA GTCAGATGAG AAGAACTGTG AAGTACTCTG TTTAATTGAT
    CAGTTCCGCT GTGCCAATGG TCAATGCATT GGAAAACACA AGAAATGTGA TCATAATTTG
    GATTGCAGTG ACAAATCAGA TGAACTGGAT TGTTATCCAA CTGAGGAGCC AGCACCACAG
    GCCACCAATA CAGTGGGTTC TGTTATTGGA GTAATTGTTA CCATCTTTGT GTCTGGAACC
    ATATACTTTA TCTGCCAGAG GATGTTGTGT CCACGGATGA AGGGCGATGG GGAAACCATG
    ACTAACGACT ATGTAGTTCA TGGACCAGCT TCTGTGCCCC TTGGTTACGT GCCACACCCC
    AGTTCTTTGT CAGGATCTCT TCCAGGAATG TCTCGTGGTA AATCAATGAT CAGTTCCCTC
    AGTATCATGG GGGGGAGCAG TGGACCCCCT TATGACCGAG CCCATGTCAC AGGAGCATCG
    TCAAGTAGTT CATCAAGCAC CAAAGGCACT TACTTCCCTG CAATTTTGAA CCCTCCGCCA
    TCCCCGGCCA CAGAGCGATC ACATTACACC ATGGAATTTG GTTATTCTTC TAACAGCCCT
    TCCACTCACA GGTCCTACAG CTACAGGCCG TACAGCTACC GGCACTTTGC GCCCCCCACC
    ACGCCCTGCA GCACAGATGT GTGTGACAGT GACTATGCTC CTAGCCGAAG GATGACCTCA
    GTGGTGACGG CCAAGGGCTA CACTAGTGAC TTGAACTATG ACTCAGAGCC TGTGCCCCCA
    CCTCCCACGC CCCGAAGCCA GTACTTGTCA GCAGAAGAGA ACTATGAAAG CTGCCCGCCT
    TCTCCATACA CGGAGAGGAG CTACTCTCAT CACCTCTACC CACCGCCACC CTCTCCCTGT
    ACAGACTCCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19705
    Clone ID Related Accession (Same CDS sequence) XM_005207029.4 , XM_015463448.1 , XM_005207029.3
    Accession Version XM_015463448.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644840.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAGTCCCAGT TCCAGTGCGC CAGTGGACAG TGTATCGATG GTGCCCTGCG ATGCAACGGA
    GACGCGAACT GCCAGGATAA GTCAGATGAG AAGAACTGTG AAGTACTCTG TTTAATTGAT
    CAGTTCCGCT GTGCCAATGG TCAATGCATT GGAAAACACA AGAAATGTGA TCATAATTTG
    GATTGCAGTG ACAAATCAGA TGAACTGGAT TGTTATCCAA CTGAGGAGCC AGCACCACAG
    GCCACCAATA CAGTGGGTTC TGTTATTGGA GTAATTGTTA CCATCTTTGT GTCTGGAACC
    ATATACTTTA TCTGCCAGAG GATGTTGTGT CCACGGATGA AGGGCGATGG GGAAACCATG
    ACTAACGACT ATGTAGTTCA TGGACCAGCT TCTGTGCCCC TTGGTTACGT GCCACACCCC
    AGTTCTTTGT CAGGATCTCT TCCAGGAATG TCTCGTGGTA AATCAATGAT CAGTTCCCTC
    AGTATCATGG GGGGGAGCAG TGGACCCCCT TATGACCGAG CCCATGTCAC AGGAGCATCG
    TCAAGTAGTT CATCAAGCAC CAAAGGCACT TACTTCCCTG CAATTTTGAA CCCTCCGCCA
    TCCCCGGCCA CAGAGCGATC ACATTACACC ATGGAATTTG GTTATTCTTC TAACAGCCCT
    TCCACTCACA GGTCCTACAG CTACAGGCCG TACAGCTACC GGCACTTTGC GCCCCCCACC
    ACGCCCTGCA GCACAGATGT GTGTGACAGT GACTATGCTC CTAGCCGAAG GATGACCTCA
    GTGGTGACGG CCAAGGGCTA CACTAGTGAC TTGAACTATG ACTCAGAGCC TGTGCCCCCA
    CCTCCCACGC CCCGAAGCCA GTACTTGTCA GCAGAAGAGA ACTATGAAAG CTGCCCGCCT
    TCTCCATACA CGGAGAGGAG CTACTCTCAT CACCTCTACC CACCGCCACC CTCTCCCTGT
    ACAGACTCCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318934.1
    CDS126..4940
    Translation

    Target ORF information:

    RefSeq Version XM_015463448.1
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAGTCCCAGT TCCAGTGCGC CAGTGGACAG TGTATCGATG GTGCCCTGCG ATGCAACGGA
    GACGCGAACT GCCAGGATAA GTCAGATGAG AAGAACTGTG AAGTACTCTG TTTAATTGAT
    CAGTTCCGCT GTGCCAATGG TCAATGCATT GGAAAACACA AGAAATGTGA TCATAATTTG
    GATTGCAGTG ACAAATCAGA TGAACTGGAT TGTTATCCAA CTGAGGAGCC AGCACCACAG
    GCCACCAATA CAGTGGGTTC TGTTATTGGA GTAATTGTTA CCATCTTTGT GTCTGGAACC
    ATATACTTTA TCTGCCAGAG GATGTTGTGT CCACGGATGA AGGGCGATGG GGAAACCATG
    ACTAACGACT ATGTAGTTCA TGGACCAGCT TCTGTGCCCC TTGGTTACGT GCCACACCCC
    AGTTCTTTGT CAGGATCTCT TCCAGGAATG TCTCGTGGTA AATCAATGAT CAGTTCCCTC
    AGTATCATGG GGGGGAGCAG TGGACCCCCT TATGACCGAG CCCATGTCAC AGGAGCATCG
    TCAAGTAGTT CATCAAGCAC CAAAGGCACT TACTTCCCTG CAATTTTGAA CCCTCCGCCA
    TCCCCGGCCA CAGAGCGATC ACATTACACC ATGGAATTTG GTTATTCTTC TAACAGCCCT
    TCCACTCACA GGTCCTACAG CTACAGGCCG TACAGCTACC GGCACTTTGC GCCCCCCACC
    ACGCCCTGCA GCACAGATGT GTGTGACAGT GACTATGCTC CTAGCCGAAG GATGACCTCA
    GTGGTGACGG CCAAGGGCTA CACTAGTGAC TTGAACTATG ACTCAGAGCC TGTGCCCCCA
    CCTCCCACGC CCCGAAGCCA GTACTTGTCA GCAGAAGAGA ACTATGAAAG CTGCCCGCCT
    TCTCCATACA CGGAGAGGAG CTACTCTCAT CACCTCTACC CACCGCCACC CTCTCCCTGT
    ACAGACTCCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19705
    Clone ID Related Accession (Same CDS sequence) XM_005207029.4 , XM_015463448.1 , XM_005207029.3
    Accession Version XM_005207029.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103936.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAGTCCCAGT TCCAGTGCGC CAGTGGACAG TGTATCGATG GTGCCCTGCG ATGCAACGGA
    GACGCGAACT GCCAGGATAA GTCAGATGAG AAGAACTGTG AAGTACTCTG TTTAATTGAT
    CAGTTCCGCT GTGCCAATGG TCAATGCATT GGAAAACACA AGAAATGTGA TCATAATTTG
    GATTGCAGTG ACAAATCAGA TGAACTGGAT TGTTATCCAA CTGAGGAGCC AGCACCACAG
    GCCACCAATA CAGTGGGTTC TGTTATTGGA GTAATTGTTA CCATCTTTGT GTCTGGAACC
    ATATACTTTA TCTGCCAGAG GATGTTGTGT CCACGGATGA AGGGCGATGG GGAAACCATG
    ACTAACGACT ATGTAGTTCA TGGACCAGCT TCTGTGCCCC TTGGTTACGT GCCACACCCC
    AGTTCTTTGT CAGGATCTCT TCCAGGAATG TCTCGTGGTA AATCAATGAT CAGTTCCCTC
    AGTATCATGG GGGGGAGCAG TGGACCCCCT TATGACCGAG CCCATGTCAC AGGAGCATCG
    TCAAGTAGTT CATCAAGCAC CAAAGGCACT TACTTCCCTG CAATTTTGAA CCCTCCGCCA
    TCCCCGGCCA CAGAGCGATC ACATTACACC ATGGAATTTG GTTATTCTTC TAACAGCCCT
    TCCACTCACA GGTCCTACAG CTACAGGCCG TACAGCTACC GGCACTTTGC GCCCCCCACC
    ACGCCCTGCA GCACAGATGT GTGTGACAGT GACTATGCTC CTAGCCGAAG GATGACCTCA
    GTGGTGACGG CCAAGGGCTA CACTAGTGAC TTGAACTATG ACTCAGAGCC TGTGCCCCCA
    CCTCCCACGC CCCGAAGCCA GTACTTGTCA GCAGAAGAGA ACTATGAAAG CTGCCCGCCT
    TCTCCATACA CGGAGAGGAG CTACTCTCAT CACCTCTACC CACCGCCACC CTCTCCCTGT
    ACAGACTCCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207086.1
    CDS126..4940
    Translation

    Target ORF information:

    RefSeq Version XM_005207029.3
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207029.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGT GTTGTGAATT ATCTGAGAGA GTTCCTTCAG
    TGTGAAGTTA TTTTTGGTGG CATCACTCTG GGATGTGTTT ATCCTTTTGG GCACAACCAC
    TTTGCTCATT TATGTTGGTT CATGTACTGG ACAGACTGGG GAGAAGTACC AAAGATAGAA
    CGTGCTGGAA TGGATGGTTC TGATCGTTTC GTCATAATAA ACACTGAAAT TTACTGGCCA
    AATGGACTGA CTTTGGATTA TGAGGAACGG AAGCTTTATT GGGCAGATGC AAAACTTAAT
    TTCATTCACC GATCAAATCT GGATGGAACA AATCGGCAGG CGGTGGTTAA AGGTTCCCTT
    CCACATCCTT TTGCCCTGAC GTTATTTGAG GACACATTGT ACTGGACTGA CTGGAATACA
    CATTCCATTT TGGCTTGCAA CAAGTACACT GGCGAGGGTC TGCGTGAAAT CCATTCTAAC
    ATCTTCTCTC CCATGGATAT ACATGTCTTC AGCCAACAGA GGCAGCCAAA TGCTACAAAT
    CCATGTGGAA TTGATAATGG TGGTTGTTCC CACTTGTGTT TGATGTCTCC AGTCAAGCCT
    TTTTATCAGT GTGCTTGCCC AACTGGGGTC ACACTCCTGG AGAACGGAAA AACCTGCAAA
    GATGGTGCCA CTGAATTACT GCTTCTAGCC CGAAGGACAG ACTTGAGACG CATTTCTTTG
    GATACACCAG ATTTCACTGA CATTGTTCTA CAGTTAGAAG ACATCCGCCA TGCCATTGCC
    ATAGATTATG ATCCCGTGGA AGGCTATATC TACTGGACTG ATGATGAAGT GAGGGCCATA
    CGCCGCTCCT TCATAGATGG ATCTGGCAGT CAGTTTGTGG TCACTGCCCA AATTGCCCAT
    CCTGATGGTA TTGCTGTTGA TTGGGTTGCC CGAAATCTTT ATTGGACAGA CACTGGCACT
    GATCGGATAG AAGTGACAAG GCTCAACGGG ACCATGAGGA AGATCTTGAT TTCAGAGGAC
    TTAGAGGAAC CCCGGGCTAT TGTGCTAGAT CCCATGGTTG GGTACATGTA TTGGACTGAC
    TGGGGAGAAA TCCCGAAAAT TGAGCGAGCA GCTTTGGATG GTTCTGATCG AGTAGTGTTG
    GTTAACACTT CTCTTGGTTG GCCAAATGGT TTAGCCTTGG ATTATGATGA AGGCAAAATA
    TACTGGGGAG ATGCCAAAAC AGACAAAATT GAGGTTATGA ATACCGATGG GACTGGGAGA
    CGAGTGCTCG TGGAAGACAA AATCCCCCAC ATATTTGGGT TTACCTTGTT GGGTGACTAT
    GTCTACTGGA CTGACTGGCA GAGGCGGAGC ATCGAAAGAG TACACAAACG AAGCGCAGAG
    CGGGAAGTCA TCATCGATCA GCTCCCTGAC CTCATGGGCC TGAAGGCCAC GAATGTTCAT
    CGAGTCATTG GTTCCAACCC CTGCGCTGAG GATAATGGGG GATGTAGCCA TCTCTGCCTC
    TATAGACCCC AGGGCCTTCG CTGTGCCTGC CCCATTGGCT TTGAACTCAT CAGTGACATG
    AAGACCTGCA TTGTCCCAGA GGCTTTCCTT CTGTTTTCAC GAAGAGCTGA CATCAGGAGA
    ATTTCTTTGG AAACTAATAA TAATAACGTG GCCATTCCAC TCACTGGTGT CAAAGAAGCT
    TCTGCTTTGG ATTTTGACGT GACAGACAAC CGGATTTACT GGACTGACAT ATCACTCAAG
    ACTATCAGCA GAGCCTTTAT GAATGGCAGT GCCCTGGAAC ATGTGGTAGA ATTTGGCTTA
    GATTATCCGG AAGGCATGGC TGTAGACTGG CTTGGGAAGA ACTTGTACTG GGCAGACACA
    GGAACCAATC GAATTGAGGT GTCAAAGTTG GATGGGCAAC ACCGACAAGT TTTGGTGTGG
    AAAGACCTAG ATAGTCCCAG AGCTCTTGCG TTGGACCCCG CTGAAGGATT TATGTATTGG
    ACCGAATGGG GTGGAAAACC TAAGATAGAC AGAGCTGCCA TGGACGGAAG TGAACGTACC
    ACGCTGGTTC CCAATGTGGG GCGGGCAAAC GGCCTGACCA TTGACTATGC TAAAAGGAGG
    CTTTATTGGA CAGACCTGGA CACCAACTTA ATAGAGTCTT CAAATATGCT TGGACTCAAC
    CGTGAAGTTA TAGCAGATGA CTTGCCTCAT CCTTTTGGCT TAACTCAGTA CCAAGATTAC
    ATCTACTGGA CGGACTGGAG CCGACGCAGC ATTGAGCGTG CCAACAAAAC CAGTGGCCAA
    AACCGCACCA TCATTCAAGG CCACTTGGAT TATGTGATGG ACATCCTCGT GTTTCACTCC
    TCACGGCAGG CAGGATGGAA CGAGTGTGCT TCCAGCAACG GGCACTGCTC CCACCTCTGC
    TTGGCCGTGC CAGTTGGAGG TTTCGTTTGT GGATGCCCTG CCCACTACTC TCTGAATGCT
    GACAACAGGA CTTGCAGTGC TCCAACTACT TTCCTGCTCT TCAGTCAGAA GAGTGCCATC
    AACCGCATGG TGATTGATGA ACAGCAGAGC CCTGACATCA TCCTTCCCAT CCACAGCCTT
    CGGAATGTCC GGGCCATTGA CTATGACCCC CTGGACAAGC AACTCTATTG GATTGATTCA
    CGACAAAACA TGATCCGAAA AGCACAAGAA GATGGCAGCC AGGGCTTTAC TGTGGTTGTG
    AGCTCAGTTC CGAATCAAAA CCTAGAAATC CAGCCCTATG ACCTCAGCAT TGATATTTAT
    AGCCGCTACA TCTATTGGAC TTGCGAGGCC ACCAATGTCA TTAATGTAAC GAGATTAGAT
    GGGAGATCAA TTGGAGTGGT GCTAAAGGGC GAGCAAGACA GACCTCGAGC CATTGTGGTA
    AACCCAGAAA AAGGGTATAT GTATTTTACC AATCTTCAGG AAAGGTCTCC CAAAATTGAA
    CGAGCTGCTT TAGATGGAAC AGAACGAGAG GTCCTCTTTT TCAGTGGTTT AAGTAAACCA
    GTTGCTTTAG CTCTTGATAG CAGATTGGGC AAACTCTTCT GGGCTGATTC AGATCTCCGA
    CGAATTGAAA GCAGTGATCT TTCAGGGGCC AACCGAATAG TATTAGAAGA CACCAACATT
    CTACAGCCTG TGGGACTGAC TGTGTTTGAA AACTGGCTGT ACTGGATCGA TAAGCAGCAG
    CAAATGATTG AAAAAATTGA CATGACAGGT CGAGAGGGTA GAACCAAAGT CCAGGCTCGA
    ATTGCCCAGC TTAGTGACAT TCACGCGGTA AAGGAGCTGA ATCTTCAGGA ATATAGACAG
    CACCCTTGTG CTCAGGATAA TGGTGGCTGT TCACATATCT GTCTTGTAAA GGGAGATGGT
    ACTACAAGGT GTTCTTGCCC CATGCACTTG GTTCTTCTTC AGGATGAGCT ATTATGTGGA
    GAGTCCCAGT TCCAGTGCGC CAGTGGACAG TGTATCGATG GTGCCCTGCG ATGCAACGGA
    GACGCGAACT GCCAGGATAA GTCAGATGAG AAGAACTGTG AAGTACTCTG TTTAATTGAT
    CAGTTCCGCT GTGCCAATGG TCAATGCATT GGAAAACACA AGAAATGTGA TCATAATTTG
    GATTGCAGTG ACAAATCAGA TGAACTGGAT TGTTATCCAA CTGAGGAGCC AGCACCACAG
    GCCACCAATA CAGTGGGTTC TGTTATTGGA GTAATTGTTA CCATCTTTGT GTCTGGAACC
    ATATACTTTA TCTGCCAGAG GATGTTGTGT CCACGGATGA AGGGCGATGG GGAAACCATG
    ACTAACGACT ATGTAGTTCA TGGACCAGCT TCTGTGCCCC TTGGTTACGT GCCACACCCC
    AGTTCTTTGT CAGGATCTCT TCCAGGAATG TCTCGTGGTA AATCAATGAT CAGTTCCCTC
    AGTATCATGG GGGGGAGCAG TGGACCCCCT TATGACCGAG CCCATGTCAC AGGAGCATCG
    TCAAGTAGTT CATCAAGCAC CAAAGGCACT TACTTCCCTG CAATTTTGAA CCCTCCGCCA
    TCCCCGGCCA CAGAGCGATC ACATTACACC ATGGAATTTG GTTATTCTTC TAACAGCCCT
    TCCACTCACA GGTCCTACAG CTACAGGCCG TACAGCTACC GGCACTTTGC GCCCCCCACC
    ACGCCCTGCA GCACAGATGT GTGTGACAGT GACTATGCTC CTAGCCGAAG GATGACCTCA
    GTGGTGACGG CCAAGGGCTA CACTAGTGAC TTGAACTATG ACTCAGAGCC TGTGCCCCCA
    CCTCCCACGC CCCGAAGCCA GTACTTGTCA GCAGAAGAGA ACTATGAAAG CTGCCCGCCT
    TCTCCATACA CGGAGAGGAG CTACTCTCAT CACCTCTACC CACCGCCACC CTCTCCCTGT
    ACAGACTCCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19706
    Clone ID Related Accession (Same CDS sequence) XM_015463449.1 , XM_003586111.4 , XM_003586111.5
    Accession Version XM_015463449.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644840.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAGTCCCA GTTCCAGTGC GCCAGTGGAC AGTGTATCGA TGGTGCCCTG
    CGATGCAACG GAGACGCGAA CTGCCAGGAT AAGTCAGATG AGAAGAACTG TGAAGTACTC
    TGTTTAATTG ATCAGTTCCG CTGTGCCAAT GGTCAATGCA TTGGAAAACA CAAGAAATGT
    GATCATAATT TGGATTGCAG TGACAAATCA GATGAACTGG ATTGTTATCC AACTGAGGAG
    CCAGCACCAC AGGCCACCAA TACAGTGGGT TCTGTTATTG GAGTAATTGT TACCATCTTT
    GTGTCTGGAA CCATATACTT TATCTGCCAG AGGATGTTGT GTCCACGGAT GAAGGGCGAT
    GGGGAAACCA TGACTAACGA CTATGTAGTT CATGGACCAG CTTCTGTGCC CCTTGGTTAC
    GTGCCACACC CCAGTTCTTT GTCAGGATCT CTTCCAGGAA TGTCTCGTGG TAAATCAATG
    ATCAGTTCCC TCAGTATCAT GGGGGGGAGC AGTGGACCCC CTTATGACCG AGCCCATGTC
    ACAGGAGCAT CGTCAAGTAG TTCATCAAGC ACCAAAGGCA CTTACTTCCC TGCAATTTTG
    AACCCTCCGC CATCCCCGGC CACAGAGCGA TCACATTACA CCATGGAATT TGGTTATTCT
    TCTAACAGCC CTTCCACTCA CAGGTCCTAC AGCTACAGGC CGTACAGCTA CCGGCACTTT
    GCGCCCCCCA CCACGCCCTG CAGCACAGAT GTGTGTGACA GTGACTATGC TCCTAGCCGA
    AGGATGACCT CAGTGGTGAC GGCCAAGGGC TACACTAGTG ACTTGAACTA TGACTCAGAG
    CCTGTGCCCC CACCTCCCAC GCCCCGAAGC CAGTACTTGT CAGCAGAAGA GAACTATGAA
    AGCTGCCCGC CTTCTCCATA CACGGAGAGG AGCTACTCTC ATCACCTCTA CCCACCGCCA
    CCCTCTCCCT GTACAGACTC CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318935.1
    CDS125..4831
    Translation

    Target ORF information:

    RefSeq Version XM_015463449.1
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAGTCCCA GTTCCAGTGC GCCAGTGGAC AGTGTATCGA TGGTGCCCTG
    CGATGCAACG GAGACGCGAA CTGCCAGGAT AAGTCAGATG AGAAGAACTG TGAAGTACTC
    TGTTTAATTG ATCAGTTCCG CTGTGCCAAT GGTCAATGCA TTGGAAAACA CAAGAAATGT
    GATCATAATT TGGATTGCAG TGACAAATCA GATGAACTGG ATTGTTATCC AACTGAGGAG
    CCAGCACCAC AGGCCACCAA TACAGTGGGT TCTGTTATTG GAGTAATTGT TACCATCTTT
    GTGTCTGGAA CCATATACTT TATCTGCCAG AGGATGTTGT GTCCACGGAT GAAGGGCGAT
    GGGGAAACCA TGACTAACGA CTATGTAGTT CATGGACCAG CTTCTGTGCC CCTTGGTTAC
    GTGCCACACC CCAGTTCTTT GTCAGGATCT CTTCCAGGAA TGTCTCGTGG TAAATCAATG
    ATCAGTTCCC TCAGTATCAT GGGGGGGAGC AGTGGACCCC CTTATGACCG AGCCCATGTC
    ACAGGAGCAT CGTCAAGTAG TTCATCAAGC ACCAAAGGCA CTTACTTCCC TGCAATTTTG
    AACCCTCCGC CATCCCCGGC CACAGAGCGA TCACATTACA CCATGGAATT TGGTTATTCT
    TCTAACAGCC CTTCCACTCA CAGGTCCTAC AGCTACAGGC CGTACAGCTA CCGGCACTTT
    GCGCCCCCCA CCACGCCCTG CAGCACAGAT GTGTGTGACA GTGACTATGC TCCTAGCCGA
    AGGATGACCT CAGTGGTGAC GGCCAAGGGC TACACTAGTG ACTTGAACTA TGACTCAGAG
    CCTGTGCCCC CACCTCCCAC GCCCCGAAGC CAGTACTTGT CAGCAGAAGA GAACTATGAA
    AGCTGCCCGC CTTCTCCATA CACGGAGAGG AGCTACTCTC ATCACCTCTA CCCACCGCCA
    CCCTCTCCCT GTACAGACTC CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19706
    Clone ID Related Accession (Same CDS sequence) XM_015463449.1 , XM_003586111.4 , XM_003586111.5
    Accession Version XM_003586111.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103936.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586111.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAGTCCCA GTTCCAGTGC GCCAGTGGAC AGTGTATCGA TGGTGCCCTG
    CGATGCAACG GAGACGCGAA CTGCCAGGAT AAGTCAGATG AGAAGAACTG TGAAGTACTC
    TGTTTAATTG ATCAGTTCCG CTGTGCCAAT GGTCAATGCA TTGGAAAACA CAAGAAATGT
    GATCATAATT TGGATTGCAG TGACAAATCA GATGAACTGG ATTGTTATCC AACTGAGGAG
    CCAGCACCAC AGGCCACCAA TACAGTGGGT TCTGTTATTG GAGTAATTGT TACCATCTTT
    GTGTCTGGAA CCATATACTT TATCTGCCAG AGGATGTTGT GTCCACGGAT GAAGGGCGAT
    GGGGAAACCA TGACTAACGA CTATGTAGTT CATGGACCAG CTTCTGTGCC CCTTGGTTAC
    GTGCCACACC CCAGTTCTTT GTCAGGATCT CTTCCAGGAA TGTCTCGTGG TAAATCAATG
    ATCAGTTCCC TCAGTATCAT GGGGGGGAGC AGTGGACCCC CTTATGACCG AGCCCATGTC
    ACAGGAGCAT CGTCAAGTAG TTCATCAAGC ACCAAAGGCA CTTACTTCCC TGCAATTTTG
    AACCCTCCGC CATCCCCGGC CACAGAGCGA TCACATTACA CCATGGAATT TGGTTATTCT
    TCTAACAGCC CTTCCACTCA CAGGTCCTAC AGCTACAGGC CGTACAGCTA CCGGCACTTT
    GCGCCCCCCA CCACGCCCTG CAGCACAGAT GTGTGTGACA GTGACTATGC TCCTAGCCGA
    AGGATGACCT CAGTGGTGAC GGCCAAGGGC TACACTAGTG ACTTGAACTA TGACTCAGAG
    CCTGTGCCCC CACCTCCCAC GCCCCGAAGC CAGTACTTGT CAGCAGAAGA GAACTATGAA
    AGCTGCCCGC CTTCTCCATA CACGGAGAGG AGCTACTCTC ATCACCTCTA CCCACCGCCA
    CCCTCTCCCT GTACAGACTC CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586159.1
    CDS125..4831
    Translation

    Target ORF information:

    RefSeq Version XM_003586111.4
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586111.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAGTCCCA GTTCCAGTGC GCCAGTGGAC AGTGTATCGA TGGTGCCCTG
    CGATGCAACG GAGACGCGAA CTGCCAGGAT AAGTCAGATG AGAAGAACTG TGAAGTACTC
    TGTTTAATTG ATCAGTTCCG CTGTGCCAAT GGTCAATGCA TTGGAAAACA CAAGAAATGT
    GATCATAATT TGGATTGCAG TGACAAATCA GATGAACTGG ATTGTTATCC AACTGAGGAG
    CCAGCACCAC AGGCCACCAA TACAGTGGGT TCTGTTATTG GAGTAATTGT TACCATCTTT
    GTGTCTGGAA CCATATACTT TATCTGCCAG AGGATGTTGT GTCCACGGAT GAAGGGCGAT
    GGGGAAACCA TGACTAACGA CTATGTAGTT CATGGACCAG CTTCTGTGCC CCTTGGTTAC
    GTGCCACACC CCAGTTCTTT GTCAGGATCT CTTCCAGGAA TGTCTCGTGG TAAATCAATG
    ATCAGTTCCC TCAGTATCAT GGGGGGGAGC AGTGGACCCC CTTATGACCG AGCCCATGTC
    ACAGGAGCAT CGTCAAGTAG TTCATCAAGC ACCAAAGGCA CTTACTTCCC TGCAATTTTG
    AACCCTCCGC CATCCCCGGC CACAGAGCGA TCACATTACA CCATGGAATT TGGTTATTCT
    TCTAACAGCC CTTCCACTCA CAGGTCCTAC AGCTACAGGC CGTACAGCTA CCGGCACTTT
    GCGCCCCCCA CCACGCCCTG CAGCACAGAT GTGTGTGACA GTGACTATGC TCCTAGCCGA
    AGGATGACCT CAGTGGTGAC GGCCAAGGGC TACACTAGTG ACTTGAACTA TGACTCAGAG
    CCTGTGCCCC CACCTCCCAC GCCCCGAAGC CAGTACTTGT CAGCAGAAGA GAACTATGAA
    AGCTGCCCGC CTTCTCCATA CACGGAGAGG AGCTACTCTC ATCACCTCTA CCCACCGCCA
    CCCTCTCCCT GTACAGACTC CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19706
    Clone ID Related Accession (Same CDS sequence) XM_015463449.1 , XM_003586111.4 , XM_003586111.5
    Accession Version XM_003586111.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product low-density lipoprotein receptor-related protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586111.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAGTCCCA GTTCCAGTGC GCCAGTGGAC AGTGTATCGA TGGTGCCCTG
    CGATGCAACG GAGACGCGAA CTGCCAGGAT AAGTCAGATG AGAAGAACTG TGAAGTACTC
    TGTTTAATTG ATCAGTTCCG CTGTGCCAAT GGTCAATGCA TTGGAAAACA CAAGAAATGT
    GATCATAATT TGGATTGCAG TGACAAATCA GATGAACTGG ATTGTTATCC AACTGAGGAG
    CCAGCACCAC AGGCCACCAA TACAGTGGGT TCTGTTATTG GAGTAATTGT TACCATCTTT
    GTGTCTGGAA CCATATACTT TATCTGCCAG AGGATGTTGT GTCCACGGAT GAAGGGCGAT
    GGGGAAACCA TGACTAACGA CTATGTAGTT CATGGACCAG CTTCTGTGCC CCTTGGTTAC
    GTGCCACACC CCAGTTCTTT GTCAGGATCT CTTCCAGGAA TGTCTCGTGG TAAATCAATG
    ATCAGTTCCC TCAGTATCAT GGGGGGGAGC AGTGGACCCC CTTATGACCG AGCCCATGTC
    ACAGGAGCAT CGTCAAGTAG TTCATCAAGC ACCAAAGGCA CTTACTTCCC TGCAATTTTG
    AACCCTCCGC CATCCCCGGC CACAGAGCGA TCACATTACA CCATGGAATT TGGTTATTCT
    TCTAACAGCC CTTCCACTCA CAGGTCCTAC AGCTACAGGC CGTACAGCTA CCGGCACTTT
    GCGCCCCCCA CCACGCCCTG CAGCACAGAT GTGTGTGACA GTGACTATGC TCCTAGCCGA
    AGGATGACCT CAGTGGTGAC GGCCAAGGGC TACACTAGTG ACTTGAACTA TGACTCAGAG
    CCTGTGCCCC CACCTCCCAC GCCCCGAAGC CAGTACTTGT CAGCAGAAGA GAACTATGAA
    AGCTGCCCGC CTTCTCCATA CACGGAGAGG AGCTACTCTC ATCACCTCTA CCCACCGCCA
    CCCTCTCCCT GTACAGACTC CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586159.1
    CDS200..4906
    Translation

    Target ORF information:

    RefSeq Version XM_003586111.5
    Organism Bos taurus(cattle)
    Definition Bos taurus LDL receptor related protein 6 (LRP6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586111.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGCCG TCCTGAGGAG CCTCCTGGCC TGCAGTTTCT GCGTGCTCCT GAGAGCGGCC 
    CCTTTGTTGC TTTATGCAAA CAGACGGGAC TTGCGATTGG TTGATGCTGC AAATGGCAAA
    GAGAATGCTA CGATCGTAGT TGGAGGCTTG GAGGATGCAG CTGCGTTGGA CTTTGTGTTT
    AGTCATGGCT TGATATACTG GAGTGATGTC AGCGAAGAGG CCATTAAACG AATAGAGTTC
    AACAAAACTG AGAGTGTACA GAATGTTGTT GTTTCTGGAT TATTGTCCCC TGATGGGCTG
    GCATGTGATT GGCTTGGAGA AAAATTGTAC TGGACAGATT CTGAAACTAA TCGTATTGAA
    GTTTCTAATT TAGATGGATC TTTACGAAAA GTTTTATTTT GGCAAGAGTT GGATCAACCT
    AGAGCAATTG CCTTAGATCC TTCAAGTGGG TTCATGTACT GGACAGACTG GGGAGAAGTA
    CCAAAGATAG AACGTGCTGG AATGGATGGT TCTGATCGTT TCGTCATAAT AAACACTGAA
    ATTTACTGGC CAAATGGACT GACTTTGGAT TATGAGGAAC GGAAGCTTTA TTGGGCAGAT
    GCAAAACTTA ATTTCATTCA CCGATCAAAT CTGGATGGAA CAAATCGGCA GGCGGTGGTT
    AAAGGTTCCC TTCCACATCC TTTTGCCCTG ACGTTATTTG AGGACACATT GTACTGGACT
    GACTGGAATA CACATTCCAT TTTGGCTTGC AACAAGTACA CTGGCGAGGG TCTGCGTGAA
    ATCCATTCTA ACATCTTCTC TCCCATGGAT ATACATGTCT TCAGCCAACA GAGGCAGCCA
    AATGCTACAA ATCCATGTGG AATTGATAAT GGTGGTTGTT CCCACTTGTG TTTGATGTCT
    CCAGTCAAGC CTTTTTATCA GTGTGCTTGC CCAACTGGGG TCACACTCCT GGAGAACGGA
    AAAACCTGCA AAGATGGTGC CACTGAATTA CTGCTTCTAG CCCGAAGGAC AGACTTGAGA
    CGCATTTCTT TGGATACACC AGATTTCACT GACATTGTTC TACAGTTAGA AGACATCCGC
    CATGCCATTG CCATAGATTA TGATCCCGTG GAAGGCTATA TCTACTGGAC TGATGATGAA
    GTGAGGGCCA TACGCCGCTC CTTCATAGAT GGATCTGGCA GTCAGTTTGT GGTCACTGCC
    CAAATTGCCC ATCCTGATGG TATTGCTGTT GATTGGGTTG CCCGAAATCT TTATTGGACA
    GACACTGGCA CTGATCGGAT AGAAGTGACA AGGCTCAACG GGACCATGAG GAAGATCTTG
    ATTTCAGAGG ACTTAGAGGA ACCCCGGGCT ATTGTGCTAG ATCCCATGGT TGGGTACATG
    TATTGGACTG ACTGGGGAGA AATCCCGAAA ATTGAGCGAG CAGCTTTGGA TGGTTCTGAT
    CGAGTAGTGT TGGTTAACAC TTCTCTTGGT TGGCCAAATG GTTTAGCCTT GGATTATGAT
    GAAGGCAAAA TATACTGGGG AGATGCCAAA ACAGACAAAA TTGAGGTTAT GAATACCGAT
    GGGACTGGGA GACGAGTGCT CGTGGAAGAC AAAATCCCCC ACATATTTGG GTTTACCTTG
    TTGGGTGACT ATGTCTACTG GACTGACTGG CAGAGGCGGA GCATCGAAAG AGTACACAAA
    CGAAGCGCAG AGCGGGAAGT CATCATCGAT CAGCTCCCTG ACCTCATGGG CCTGAAGGCC
    ACGAATGTTC ATCGAGTCAT TGGTTCCAAC CCCTGCGCTG AGGATAATGG GGGATGTAGC
    CATCTCTGCC TCTATAGACC CCAGGGCCTT CGCTGTGCCT GCCCCATTGG CTTTGAACTC
    ATCAGTGACA TGAAGACCTG CATTGTCCCA GAGGCTTTCC TTCTGTTTTC ACGAAGAGCT
    GACATCAGGA GAATTTCTTT GGAAACTAAT AATAATAACG TGGCCATTCC ACTCACTGGT
    GTCAAAGAAG CTTCTGCTTT GGATTTTGAC GTGACAGACA ACCGGATTTA CTGGACTGAC
    ATATCACTCA AGACTATCAG CAGAGCCTTT ATGAATGGCA GTGCCCTGGA ACATGTGGTA
    GAATTTGGCT TAGATTATCC GGAAGGCATG GCTGTAGACT GGCTTGGGAA GAACTTGTAC
    TGGGCAGACA CAGGAACCAA TCGAATTGAG GTGTCAAAGT TGGATGGGCA ACACCGACAA
    GTTTTGGTGT GGAAAGACCT AGATAGTCCC AGAGCTCTTG CGTTGGACCC CGCTGAAGGA
    TTTATGTATT GGACCGAATG GGGTGGAAAA CCTAAGATAG ACAGAGCTGC CATGGACGGA
    AGTGAACGTA CCACGCTGGT TCCCAATGTG GGGCGGGCAA ACGGCCTGAC CATTGACTAT
    GCTAAAAGGA GGCTTTATTG GACAGACCTG GACACCAACT TAATAGAGTC TTCAAATATG
    CTTGGACTCA ACCGTGAAGT TATAGCAGAT GACTTGCCTC ATCCTTTTGG CTTAACTCAG
    TACCAAGATT ACATCTACTG GACGGACTGG AGCCGACGCA GCATTGAGCG TGCCAACAAA
    ACCAGTGGCC AAAACCGCAC CATCATTCAA GGCCACTTGG ATTATGTGAT GGACATCCTC
    GTGTTTCACT CCTCACGGCA GGCAGGATGG AACGAGTGTG CTTCCAGCAA CGGGCACTGC
    TCCCACCTCT GCTTGGCCGT GCCAGTTGGA GGTTTCGTTT GTGGATGCCC TGCCCACTAC
    TCTCTGAATG CTGACAACAG GACTTGCAGT GCTCCAACTA CTTTCCTGCT CTTCAGTCAG
    AAGAGTGCCA TCAACCGCAT GGTGATTGAT GAACAGCAGA GCCCTGACAT CATCCTTCCC
    ATCCACAGCC TTCGGAATGT CCGGGCCATT GACTATGACC CCCTGGACAA GCAACTCTAT
    TGGATTGATT CACGACAAAA CATGATCCGA AAAGCACAAG AAGATGGCAG CCAGGGCTTT
    ACTGTGGTTG TGAGCTCAGT TCCGAATCAA AACCTAGAAA TCCAGCCCTA TGACCTCAGC
    ATTGATATTT ATAGCCGCTA CATCTATTGG ACTTGCGAGG CCACCAATGT CATTAATGTA
    ACGAGATTAG ATGGGAGATC AATTGGAGTG GTGCTAAAGG GCGAGCAAGA CAGACCTCGA
    GCCATTGTGG TAAACCCAGA AAAAGGGTAT ATGTATTTTA CCAATCTTCA GGAAAGGTCT
    CCCAAAATTG AACGAGCTGC TTTAGATGGA ACAGAACGAG AGGTCCTCTT TTTCAGTGGT
    TTAAGTAAAC CAGTTGCTTT AGCTCTTGAT AGCAGATTGG GCAAACTCTT CTGGGCTGAT
    TCAGATCTCC GACGAATTGA AAGCAGTGAT CTTTCAGGGG CCAACCGAAT AGTATTAGAA
    GACACCAACA TTCTACAGCC TGTGGGACTG ACTGTGTTTG AAAACTGGCT GTACTGGATC
    GATAAGCAGC AGCAAATGAT TGAAAAAATT GACATGACAG GTCGAGAGGG TAGAACCAAA
    GTCCAGGCTC GAATTGCCCA GCTTAGTGAC ATTCACGCGG TAAAGGAGCT GAATCTTCAG
    GAATATAGAC AGCACCCTTG TGCTCAGGAT AATGGTGGCT GTTCACATAT CTGTCTTGTA
    AAGGGAGATG GTACTACAAG GTGTTCTTGC CCCATGCACT TGGTTCTTCT TCAGGATGAG
    CTATTATGTG GAGAGTCCCA GTTCCAGTGC GCCAGTGGAC AGTGTATCGA TGGTGCCCTG
    CGATGCAACG GAGACGCGAA CTGCCAGGAT AAGTCAGATG AGAAGAACTG TGAAGTACTC
    TGTTTAATTG ATCAGTTCCG CTGTGCCAAT GGTCAATGCA TTGGAAAACA CAAGAAATGT
    GATCATAATT TGGATTGCAG TGACAAATCA GATGAACTGG ATTGTTATCC AACTGAGGAG
    CCAGCACCAC AGGCCACCAA TACAGTGGGT TCTGTTATTG GAGTAATTGT TACCATCTTT
    GTGTCTGGAA CCATATACTT TATCTGCCAG AGGATGTTGT GTCCACGGAT GAAGGGCGAT
    GGGGAAACCA TGACTAACGA CTATGTAGTT CATGGACCAG CTTCTGTGCC CCTTGGTTAC
    GTGCCACACC CCAGTTCTTT GTCAGGATCT CTTCCAGGAA TGTCTCGTGG TAAATCAATG
    ATCAGTTCCC TCAGTATCAT GGGGGGGAGC AGTGGACCCC CTTATGACCG AGCCCATGTC
    ACAGGAGCAT CGTCAAGTAG TTCATCAAGC ACCAAAGGCA CTTACTTCCC TGCAATTTTG
    AACCCTCCGC CATCCCCGGC CACAGAGCGA TCACATTACA CCATGGAATT TGGTTATTCT
    TCTAACAGCC CTTCCACTCA CAGGTCCTAC AGCTACAGGC CGTACAGCTA CCGGCACTTT
    GCGCCCCCCA CCACGCCCTG CAGCACAGAT GTGTGTGACA GTGACTATGC TCCTAGCCGA
    AGGATGACCT CAGTGGTGAC GGCCAAGGGC TACACTAGTG ACTTGAACTA TGACTCAGAG
    CCTGTGCCCC CACCTCCCAC GCCCCGAAGC CAGTACTTGT CAGCAGAAGA GAACTATGAA
    AGCTGCCCGC CTTCTCCATA CACGGAGAGG AGCTACTCTC ATCACCTCTA CCCACCGCCA
    CCCTCTCCCT GTACAGACTC CTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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