FTO cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	FTO
Entrez Gene ID	618622
Full Name	FTO, alpha-ketoglutarate dependent dioxygenase
General protein information	Preferred Names FTO, alpha-ketoglutarate dependent dioxygenase Names alpha-ketoglutarate-dependent dioxygenase FTO fat mass and obesity associated
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015475778.1	XP_015331264.1	alpha-ketoglutarate-dependent dioxygenase FTO isoform X2
NM_001098142.1	NP_001091611.1	alpha-ketoglutarate-dependent dioxygenase FTO
XM_015475778.2	XP_015331264.1	alpha-ketoglutarate-dependent dioxygenase FTO isoform X2
XM_024978596.1	XP_024834364.1	alpha-ketoglutarate-dependent dioxygenase FTO isoform X3
XM_010814570.2	XP_010812872.1	alpha-ketoglutarate-dependent dioxygenase FTO isoform X1
XM_010814570.3	XP_010812872.1	alpha-ketoglutarate-dependent dioxygenase FTO isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	fto	NP_001017273.1
Homo sapiens (human)	FTO	NP_001073901.1
Pan troglodytes (chimpanzee)	FTO	XP_510968.2
Gallus gallus (chicken)	FTO	NP_001172076.1
Bos taurus (cattle)	FTO	NP_001091611.1
Rattus norvegicus (Norway rat)	Fto	NP_001034802.1
	FTO	XP_001092038.1
Danio rerio (zebrafish)	fto	XP_001345910.4
Canis lupus familiaris (dog)	FTO	XP_535301.4
Mus musculus (house mouse)	Fto	NP_036066.2

Gene Ontology
Bibliography

Related articles in PubMed

FTO gene variants are associated with growth and carcass traits in cattle.
Jevsinek Skok D, Kunej T, Kovac M, Malovrh S, Potocnik K, Petric N, Zgur S, Dovc P, Horvat S
Animal genetics47(2)219-22(2016 Apr)

Impact of variation at the FTO locus on milk fat yield in Holstein dairy cattle.
Zielke LG, Bortfeldt RH, Reissmann M, Tetens J, Thaller G, Brockmann GA
PloS one8(5)e63406(2013)

Allelic polymorphism detected in the bovine FTO gene.
Zhang B, Zhang Y, Zhang L, Wang J, Li Z, Chen H
Molecular biotechnology49(3)257-62(2011 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

34 FTO polymorphisms revealed significant association of FTO variants with lean meat percentage in Simmental and Brown cattle breeds.
Title: FTO gene variants are associated with growth and carcass traits in cattle.

association signals not only provided evidence for at least two causative mutations in the FTO locus with a functional effect on milk but also milk protein yield
Title: Impact of variation at the FTO locus on milk fat yield in Holstein dairy cattle.

Haplotype frequencies and linkage disequilibrium (LD) coefficients of FTO single nucleotide polymorphisms in three Chinese indigenous cattle breeds were analyzed.
Title: Allelic polymorphism detected in the bovine FTO gene.

The following FTO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FTO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41243	NM_001098142.1 Latest version!	Bos taurus FTO alpha-ketoglutarate dependent dioxygenase (FTO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa28519	XM_010814570.3 Latest version!	Bos taurus FTO, alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa225211	XM_024978596.1 Latest version!	Bos taurus FTO, alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa93376	XM_015475778.2 Latest version!	Bos taurus FTO, alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OBa93376	XM_015475778.1	Bos taurus fat mass and obesity associated (FTO), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa28519	XM_010814570.2	Bos taurus fat mass and obesity associated (FTO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FTO ( NM_001098142.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001091611.1 Bos taurus FTO alpha-ketoglutarate dependent dioxygenase (FTO), mRNA.

Price & Availability↑

CloneID	OBa41243
Clone ID Related Accession (Same CDS sequence)	NM_001098142.1
Accession Version	NM_001098142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-29
Organism	Bos taurus(cattle)
Product	alpha-ketoglutarate-dependent dioxygenase FTO
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140478.1. On May 23, 2007 this sequence version replaced XM_870955.2. ##Evidence-Data-START## Transcript exon combination :: BC140478.1, HM777021.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCGGA CCCCGACGGC CGAGGAACGA GAGCGCGAAG CTAAGAAACT GAGGCTTCTT 
GAAGAGCTTG AAGACACGTG GCTTCCCTAC CTGACCCCCA AAGACGATGA ATTCTATCAG 
CAGTGGCAAC TGAAATATCC TAAACTAATT CTCCGAGAAG CTGCCAGCGT GCCTGAGTTG 
CTCCATAAAG AGGTTCAACA AGCCTTTCTC ACGCTGCACA AGCACGGCTG TTTATTTCGG 
GATCTGGTGA GGATCCAGGG CAAAGACTTG CTCACTCCAG TCTCTCGCAT CCTCATCGGT 
AACCCCGGCT GCACCTACAA GTACCTGAAC ACCAGGCTCT TCACGGTACC CTGGCCCGTG 
AAAGGCTCTG ATGCAAAGTA CAATGAGGCC GAAATAGCTG CCGCCTGTCA AACGTTCCTC 
AAGCTCAACA GCTACCTGCA GGTAGAGACC ATCCAGGCTT TGGAAGAGCT TGCTGCCAAG 
GAGAAAGCCA ACATCGATGC CGTGCCAGTG TGCATAGGTC CAGATTTCCC CAGGGTTGGC 
ATGGGGTCCT CCTTTGACGG GCACGATGAG ATTGACATGA AGAACCGAGC AGCGTACAAC 
GTCACTTTGT TGAATTTCAT GGATCCCCAG AAGATGCCAT ACCTGAAAGA GGAACCGTAT 
TTTGGCATGG GGAAAATGGC CGTGAGCTGG CATCATGATG AAAATCTGGT GGACAGGTCA 
GCGGTGGCAG TGTACAGTTA TAGCTGTGAA GGCCCTGAAG AGGAAAGTGA GGACGACCCT 
CAGCTTGAAG GCAGAGATCC TGATATTTGG CACGTTGGTT TTAAGATCTC GTGGGACATA 
GAGACACCTG GTTTGGCGAT ACCCCTTCAC CAAGGAGACT GCTATTTTAT GCTTGATGAT 
CTCAATGCCA CCCACCAACA CTGTGTTTTG GCTGGTTTAC CACCTCGGTT TAGTTCCACC 
CACCGAGTGG CAGAGTGCTC AACAGGGACC TTGGAGTACA TCTTACAGCG CTGCCAGGTG 
GCCCTGCAGA ATGTCCGCGA GGAGGCAGAC AACGGTGAAA TCTCTTTGAA ATCCTTGGAG 
TCAGTGGTTT TGAAACAAGG AGAAGAAATC CACAACGAGG TCGAGTTTGA ATGGCTGAGA 
CAGTTTTGGT TTCAAGGCAG TCGATACAAA AAGTGCACTG ACTGGTGGTG TCAGCCCATG 
AGTCAGCTGG AAGAGATGTG GAGAAAGATG GAGTGGTTGA CAAGCGCTGT GCTTCGCGAA 
GTTAGAAGAG AGGGGGTCCC CATGGAACAA AAGAATGAGA TGTTGACATC CATCCTCGCC 
TCGATCACCA CTCGCCAGAA CCTGAGGAGA GAATGGCATG CCAGGTGCCA GTCTCGAATT 
GCCCGAACTT TACCCGCTGA TCAGAAGCCA GAATGCCGGC CGTACTGGGA AAAGGGTGAT 
CCTTCGATGC CTCTGCCATT TGATCTCACA GAGATCGTTT CTGAACTCAG AGGTCTGCTT 
CTGGAAACCA GGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091611.1
CDS	19..1536
Translation	MKRTPTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREAASVPELLHKEVQQAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSDAKYNEAEIAAACQTFLKLNSYLQVETIQALEELAAKEKANIDAVPVCIGPDFPRVGMGSSFDGHDEIDMKNRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDPQLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGLPPRFSSTHRVAECSTGTLEYILQRCQVALQNVREEADNGEISLKSLESVVLKQGEEIHNEVEFEWLRQFWFQGSRYKKCTDWWCQPMSQLEEMWRKMEWLTSAVLREVRREGVPMEQKNEMLTSILASITTRQNLRREWHARCQSRIARTLPADQKPECRPYWEKGDPSMPLPFDLTEIVSELRGLLLETRP

Target ORF information:

RefSeq Version	NM_001098142.1
Organism	Bos taurus(cattle)
Definition	Bos taurus FTO alpha-ketoglutarate dependent dioxygenase (FTO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098142.1

ORF Insert Sequence:

ATGAAGCGGA CCCCGACGGC CGAGGAACGA GAGCGCGAAG CTAAGAAACT GAGGCTTCTT 
GAAGAGCTTG AAGACACGTG GCTTCCCTAC CTGACCCCCA AAGACGATGA ATTCTATCAG 
CAGTGGCAAC TGAAATATCC TAAACTAATT CTCCGAGAAG CTGCCAGCGT GCCTGAGTTG 
CTCCATAAAG AGGTTCAACA AGCCTTTCTC ACGCTGCACA AGCACGGCTG TTTATTTCGG 
GATCTGGTGA GGATCCAGGG CAAAGACTTG CTCACTCCAG TCTCTCGCAT CCTCATCGGT 
AACCCCGGCT GCACCTACAA GTACCTGAAC ACCAGGCTCT TCACGGTACC CTGGCCCGTG 
AAAGGCTCTG ATGCAAAGTA CAATGAGGCC GAAATAGCTG CCGCCTGTCA AACGTTCCTC 
AAGCTCAACA GCTACCTGCA GGTAGAGACC ATCCAGGCTT TGGAAGAGCT TGCTGCCAAG 
GAGAAAGCCA ACATCGATGC CGTGCCAGTG TGCATAGGTC CAGATTTCCC CAGGGTTGGC 
ATGGGGTCCT CCTTTGACGG GCACGATGAG ATTGACATGA AGAACCGAGC AGCGTACAAC 
GTCACTTTGT TGAATTTCAT GGATCCCCAG AAGATGCCAT ACCTGAAAGA GGAACCGTAT 
TTTGGCATGG GGAAAATGGC CGTGAGCTGG CATCATGATG AAAATCTGGT GGACAGGTCA 
GCGGTGGCAG TGTACAGTTA TAGCTGTGAA GGCCCTGAAG AGGAAAGTGA GGACGACCCT 
CAGCTTGAAG GCAGAGATCC TGATATTTGG CACGTTGGTT TTAAGATCTC GTGGGACATA 
GAGACACCTG GTTTGGCGAT ACCCCTTCAC CAAGGAGACT GCTATTTTAT GCTTGATGAT 
CTCAATGCCA CCCACCAACA CTGTGTTTTG GCTGGTTTAC CACCTCGGTT TAGTTCCACC 
CACCGAGTGG CAGAGTGCTC AACAGGGACC TTGGAGTACA TCTTACAGCG CTGCCAGGTG 
GCCCTGCAGA ATGTCCGCGA GGAGGCAGAC AACGGTGAAA TCTCTTTGAA ATCCTTGGAG 
TCAGTGGTTT TGAAACAAGG AGAAGAAATC CACAACGAGG TCGAGTTTGA ATGGCTGAGA 
CAGTTTTGGT TTCAAGGCAG TCGATACAAA AAGTGCACTG ACTGGTGGTG TCAGCCCATG 
AGTCAGCTGG AAGAGATGTG GAGAAAGATG GAGTGGTTGA CAAGCGCTGT GCTTCGCGAA 
GTTAGAAGAG AGGGGGTCCC CATGGAACAA AAGAATGAGA TGTTGACATC CATCCTCGCC 
TCGATCACCA CTCGCCAGAA CCTGAGGAGA GAATGGCATG CCAGGTGCCA GTCTCGAATT 
GCCCGAACTT TACCCGCTGA TCAGAAGCCA GAATGCCGGC CGTACTGGGA AAAGGGTGAT 
CCTTCGATGC CTCTGCCATT TGATCTCACA GAGATCGTTT CTGAACTCAG AGGTCTGCTT 
CTGGAAACCA GGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FTO ( XM_010814570.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812872.1 Bos taurus fat mass and obesity associated (FTO), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28519
Clone ID Related Accession (Same CDS sequence)	XM_010814570.2 , XM_010814570.3
Accession Version	XM_010814570.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alpha-ketoglutarate-dependent dioxygenase FTO isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGTGCCAGT CTCGAATTGC CCGAACTTTA CCCGCTGATC AGAAGCCAGA ATGCCGGCCG 
TACTGGGAAA AGGGTGATCC TTCGATGCCT CTGCCATTTG ATCTCACAGA GATCGTTTCT 
GAACTCAGAG GTCTGCTTCT GGAAACCAGG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812872.1
CDS	206..1741
Translation	MHRRDKHRKTRKQIKVICFEEKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREAASVPELLHKEVQQAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSDAKYNEAEIAAACQTFLKLNSYLQVETIQALEELAAKEKANIDAVPVCIGPDFPRVGMGSSFDGHDEIDMKNRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDPQLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGLPPRFSSTHRVAECSTGTLEYILQRCQVALQNVREEADNGEISLKSLESVVLKQGEEIHNEVEFEWLRQFWFQGSRYKKCTDWWCQPMSQLEEMWRKMEWLTSAVLREVRREGVPMEQKNEMLTSILASITTRQNLRREWHARCQSRIARTLPADQKPECRPYWEKGDPSMPLPFDLTEIVSELRGLLLETRP

Target ORF information:

RefSeq Version	XM_010814570.2
Organism	Bos taurus(cattle)
Definition	Bos taurus fat mass and obesity associated (FTO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814570.2

ORF Insert Sequence:

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGTGCCAGT CTCGAATTGC CCGAACTTTA CCCGCTGATC AGAAGCCAGA ATGCCGGCCG 
TACTGGGAAA AGGGTGATCC TTCGATGCCT CTGCCATTTG ATCTCACAGA GATCGTTTCT 
GAACTCAGAG GTCTGCTTCT GGAAACCAGG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FTO ( XM_010814570.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010812872.1 Bos taurus FTO, alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28519
Clone ID Related Accession (Same CDS sequence)	XM_010814570.2 , XM_010814570.3
Accession Version	XM_010814570.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	alpha-ketoglutarate-dependent dioxygenase FTO isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814570.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGTGCCAGT CTCGAATTGC CCGAACTTTA CCCGCTGATC AGAAGCCAGA ATGCCGGCCG 
TACTGGGAAA AGGGTGATCC TTCGATGCCT CTGCCATTTG ATCTCACAGA GATCGTTTCT 
GAACTCAGAG GTCTGCTTCT GGAAACCAGG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010812872.1
CDS	160..1695
Translation	MHRRDKHRKTRKQIKVICFEEKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREAASVPELLHKEVQQAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSDAKYNEAEIAAACQTFLKLNSYLQVETIQALEELAAKEKANIDAVPVCIGPDFPRVGMGSSFDGHDEIDMKNRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDPQLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGLPPRFSSTHRVAECSTGTLEYILQRCQVALQNVREEADNGEISLKSLESVVLKQGEEIHNEVEFEWLRQFWFQGSRYKKCTDWWCQPMSQLEEMWRKMEWLTSAVLREVRREGVPMEQKNEMLTSILASITTRQNLRREWHARCQSRIARTLPADQKPECRPYWEKGDPSMPLPFDLTEIVSELRGLLLETRP

Target ORF information:

RefSeq Version	XM_010814570.3
Organism	Bos taurus(cattle)
Definition	Bos taurus FTO, alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814570.3

ORF Insert Sequence:

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGTGCCAGT CTCGAATTGC CCGAACTTTA CCCGCTGATC AGAAGCCAGA ATGCCGGCCG 
TACTGGGAAA AGGGTGATCC TTCGATGCCT CTGCCATTTG ATCTCACAGA GATCGTTTCT 
GAACTCAGAG GTCTGCTTCT GGAAACCAGG CCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FTO ( XM_024978596.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834364.1 Bos taurus FTO, alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa225211
Clone ID Related Accession (Same CDS sequence)	XM_024978596.1
Accession Version	XM_024978596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	alpha-ketoglutarate-dependent dioxygenase FTO isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834364.1
CDS	160..1548
Translation	MHRRDKHRKTRKQIKVICFEEKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREAASVPELLHKEVQQAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSDAKYNEAEIAAACQTFLKLNSYLQVETIQALEELAAKEKANIDAVPVCIGPDFPRVGMGSSFDGHDEIDMKNRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDPQLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGLPPRFSSTHRVAECSTGTLEYILQRCQVALQNVREEADNGEISLKSLESVVLKQGEEIHNEVEFEWLRQFWFQGSRYKKCTDWWCQPMSQLEEMWRKMEWLTSAVLREVRREGVPMEQKNEMLTSILASITTRQNLRREWHARL

Target ORF information:

RefSeq Version	XM_024978596.1
Organism	Bos taurus(cattle)
Definition	Bos taurus FTO, alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978596.1

ORF Insert Sequence:

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FTO ( XM_015475778.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331264.1 Bos taurus fat mass and obesity associated (FTO), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa93376
Clone ID Related Accession (Same CDS sequence)	XM_015475778.1 , XM_015475778.2
Accession Version	XM_015475778.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	alpha-ketoglutarate-dependent dioxygenase FTO isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGGTCTTCA ACCTTGTGGA AAGGGCATCA AGGTACCACT GGAAATCCAA GCCACCACTT 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331264.1
CDS	209..1654
Translation	MHRRDKHRKTRKQIKVICFEEKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREAASVPELLHKEVQQAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSDAKYNEAEIAAACQTFLKLNSYLQVETIQALEELAAKEKANIDAVPVCIGPDFPRVGMGSSFDGHDEIDMKNRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDPQLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGLPPRFSSTHRVAECSTGTLEYILQRCQVALQNVREEADNGEISLKSLESVVLKQGEEIHNEVEFEWLRQFWFQGSRYKKCTDWWCQPMSQLEEMWRKMEWLTSAVLREVRREGVPMEQKNEMLTSILASITTRQNLRREWHARVFNLVERASRYHWKSKPPLG

Target ORF information:

RefSeq Version	XM_015475778.1
Organism	Bos taurus(cattle)
Definition	Bos taurus fat mass and obesity associated (FTO), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475778.1

ORF Insert Sequence:

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGGTCTTCA ACCTTGTGGA AAGGGCATCA AGGTACCACT GGAAATCCAA GCCACCACTT 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FTO ( XM_015475778.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331264.1 Bos taurus FTO, alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa93376
Clone ID Related Accession (Same CDS sequence)	XM_015475778.1 , XM_015475778.2
Accession Version	XM_015475778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	alpha-ketoglutarate-dependent dioxygenase FTO isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGGTCTTCA ACCTTGTGGA AAGGGCATCA AGGTACCACT GGAAATCCAA GCCACCACTT 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331264.1
CDS	160..1605
Translation	MHRRDKHRKTRKQIKVICFEEKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREAASVPELLHKEVQQAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSDAKYNEAEIAAACQTFLKLNSYLQVETIQALEELAAKEKANIDAVPVCIGPDFPRVGMGSSFDGHDEIDMKNRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGPEEESEDDPQLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGLPPRFSSTHRVAECSTGTLEYILQRCQVALQNVREEADNGEISLKSLESVVLKQGEEIHNEVEFEWLRQFWFQGSRYKKCTDWWCQPMSQLEEMWRKMEWLTSAVLREVRREGVPMEQKNEMLTSILASITTRQNLRREWHARVFNLVERASRYHWKSKPPLG

Target ORF information:

RefSeq Version	XM_015475778.2
Organism	Bos taurus(cattle)
Definition	Bos taurus FTO, alpha-ketoglutarate dependent dioxygenase (FTO), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475778.2

ORF Insert Sequence:

ATGCACAGAA GGGACAAGCA TAGAAAGACC AGAAAGCAAA TAAAAGTCAT CTGCTTTGAA 
GAGAAACTGA GGCTTCTTGA AGAGCTTGAA GACACGTGGC TTCCCTACCT GACCCCCAAA 
GACGATGAAT TCTATCAGCA GTGGCAACTG AAATATCCTA AACTAATTCT CCGAGAAGCT 
GCCAGCGTGC CTGAGTTGCT CCATAAAGAG GTTCAACAAG CCTTTCTCAC GCTGCACAAG 
CACGGCTGTT TATTTCGGGA TCTGGTGAGG ATCCAGGGCA AAGACTTGCT CACTCCAGTC 
TCTCGCATCC TCATCGGTAA CCCCGGCTGC ACCTACAAGT ACCTGAACAC CAGGCTCTTC 
ACGGTACCCT GGCCCGTGAA AGGCTCTGAT GCAAAGTACA ATGAGGCCGA AATAGCTGCC 
GCCTGTCAAA CGTTCCTCAA GCTCAACAGC TACCTGCAGG TAGAGACCAT CCAGGCTTTG 
GAAGAGCTTG CTGCCAAGGA GAAAGCCAAC ATCGATGCCG TGCCAGTGTG CATAGGTCCA 
GATTTCCCCA GGGTTGGCAT GGGGTCCTCC TTTGACGGGC ACGATGAGAT TGACATGAAG 
AACCGAGCAG CGTACAACGT CACTTTGTTG AATTTCATGG ATCCCCAGAA GATGCCATAC 
CTGAAAGAGG AACCGTATTT TGGCATGGGG AAAATGGCCG TGAGCTGGCA TCATGATGAA 
AATCTGGTGG ACAGGTCAGC GGTGGCAGTG TACAGTTATA GCTGTGAAGG CCCTGAAGAG 
GAAAGTGAGG ACGACCCTCA GCTTGAAGGC AGAGATCCTG ATATTTGGCA CGTTGGTTTT 
AAGATCTCGT GGGACATAGA GACACCTGGT TTGGCGATAC CCCTTCACCA AGGAGACTGC 
TATTTTATGC TTGATGATCT CAATGCCACC CACCAACACT GTGTTTTGGC TGGTTTACCA 
CCTCGGTTTA GTTCCACCCA CCGAGTGGCA GAGTGCTCAA CAGGGACCTT GGAGTACATC 
TTACAGCGCT GCCAGGTGGC CCTGCAGAAT GTCCGCGAGG AGGCAGACAA CGGTGAAATC 
TCTTTGAAAT CCTTGGAGTC AGTGGTTTTG AAACAAGGAG AAGAAATCCA CAACGAGGTC 
GAGTTTGAAT GGCTGAGACA GTTTTGGTTT CAAGGCAGTC GATACAAAAA GTGCACTGAC 
TGGTGGTGTC AGCCCATGAG TCAGCTGGAA GAGATGTGGA GAAAGATGGA GTGGTTGACA 
AGCGCTGTGC TTCGCGAAGT TAGAAGAGAG GGGGTCCCCA TGGAACAAAA GAATGAGATG 
TTGACATCCA TCCTCGCCTC GATCACCACT CGCCAGAACC TGAGGAGAGA ATGGCATGCC 
AGGGTCTTCA ACCTTGTGGA AAGGGCATCA AGGTACCACT GGAAATCCAA GCCACCACTT 
GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FTO gene variants are associated with growth and carcass traits in cattle.
Animal genetics47(2)219-22(2016 Apr)
Jevsinek Skok D,Kunej T,Kovac M,Malovrh S,Potocnik K,Petric N,Zgur S,Dovc P,Horvat S

Impact of variation at the FTO locus on milk fat yield in Holstein dairy cattle.
PloS one8(5)e63406(2013)
Zielke LG,Bortfeldt RH,Reissmann M,Tetens J,Thaller G,Brockmann GA

Allelic polymorphism detected in the bovine FTO gene.
Molecular biotechnology49(3)257-62(2011 Nov)
Zhang B,Zhang Y,Zhang L,Wang J,Li Z,Chen H

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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FTO cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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