fto cDNA ORF clone, Danio rerio(zebrafish)

The following fto gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fto cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa13251 XM_001345874.7
Latest version!
Danio rerio fat mass and obesity associated (fto), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa41921 XM_009303351.3
Latest version!
Danio rerio fat mass and obesity associated (fto), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa144930 XM_021471998.1
Latest version!
Danio rerio fat mass and obesity associated (fto), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
ODa144931 XM_021471999.1
Latest version!
Danio rerio fat mass and obesity associated (fto), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
ODa41921 XM_009303351.2 Danio rerio fat mass and obesity associated (fto), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa13251 XM_001345874.6 Danio rerio fat mass and obesity associated (fto), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa13251
    Clone ID Related Accession (Same CDS sequence) XM_001345874.6 , XM_001345874.7
    Accession Version XM_001345874.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alpha-ketoglutarate-dependent dioxygenase FTO isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001345874.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCTT CGGTGAAGGA GGACGTGGAA
    AAAGAGAAGA CGTGTTGGCG AGTTGGTCTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGAATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001345910.4
    CDS140..1879
    Translation

    Target ORF information:

    RefSeq Version XM_001345874.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio fat mass and obesity associated (fto), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001345874.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCTT CGGTGAAGGA GGACGTGGAA
    AAAGAGAAGA CGTGTTGGCG AGTTGGTCTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGAATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13251
    Clone ID Related Accession (Same CDS sequence) XM_001345874.6 , XM_001345874.7
    Accession Version XM_001345874.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-ketoglutarate-dependent dioxygenase FTO isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001345874.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCTT CGGTGAAGGA GGACGTGGAA
    AAAGAGAAGA CGTGTTGGCG AGTTGGTCTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGAATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001345910.4
    CDS172..1911
    Translation

    Target ORF information:

    RefSeq Version XM_001345874.7
    Organism Danio rerio(zebrafish)
    Definition Danio rerio fat mass and obesity associated (fto), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001345874.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCTT CGGTGAAGGA GGACGTGGAA
    AAAGAGAAGA CGTGTTGGCG AGTTGGTCTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGAATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41921
    Clone ID Related Accession (Same CDS sequence) XM_009303351.3 , XM_009303351.2
    Accession Version XM_009303351.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-ketoglutarate-dependent dioxygenase FTO isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCTT CGGTGAAGGA GGACGTGGAA
    AAAGAGAAGA CGTGTTGGCG AGTTGGTCTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGGTGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301626.1
    CDS173..1915
    Translation

    Target ORF information:

    RefSeq Version XM_009303351.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio fat mass and obesity associated (fto), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303351.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCTT CGGTGAAGGA GGACGTGGAA
    AAAGAGAAGA CGTGTTGGCG AGTTGGTCTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGGTGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41921
    Clone ID Related Accession (Same CDS sequence) XM_009303351.3 , XM_009303351.2
    Accession Version XM_009303351.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alpha-ketoglutarate-dependent dioxygenase FTO isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCTT CGGTGAAGGA GGACGTGGAA
    AAAGAGAAGA CGTGTTGGCG AGTTGGTCTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGGTGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301626.1
    CDS140..1882
    Translation

    Target ORF information:

    RefSeq Version XM_009303351.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio fat mass and obesity associated (fto), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303351.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCTT CGGTGAAGGA GGACGTGGAA
    AAAGAGAAGA CGTGTTGGCG AGTTGGTCTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGGTGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144930
    Clone ID Related Accession (Same CDS sequence) XM_021471998.1
    Accession Version XM_021471998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-ketoglutarate-dependent dioxygenase FTO isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394700.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCCT CGGTGAAGGA GGACGTGGAG
    AAAGAGAAGA CGTGTTGGCG AGTTGGTTTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGGTGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327673.1
    CDS174..1916
    Translation

    Target ORF information:

    RefSeq Version XM_021471998.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio fat mass and obesity associated (fto), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCCT CGGTGAAGGA GGACGTGGAG
    AAAGAGAAGA CGTGTTGGCG AGTTGGTTTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGGTGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144931
    Clone ID Related Accession (Same CDS sequence) XM_021471999.1
    Accession Version XM_021471999.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-ketoglutarate-dependent dioxygenase FTO isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394700.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCCT CGGTGAAGGA GGACGTGGAG
    AAAGAGAAGA CGTGTTGGCG AGTTGGTTTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGAATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327674.1
    CDS174..1913
    Translation

    Target ORF information:

    RefSeq Version XM_021471999.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio fat mass and obesity associated (fto), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAACCGA GGCAGCGTAA ACAGTACTTC AGGAACATGA AAAGATCGGA TGACAGTGAG 
    AGGGAAAAGA GGAGAAAGAG GCGAAGGCTT CTACAGGAAC TAGGAGAGCA GAGGATTCCA
    TATCTTTCCC CAACAGACCC AGGATTTCAG GACCTGTGGG ACTCCAGTTA TGCGGGCCTG
    GCTTTGCGGC AGTCTGGTAC CCTTCCAGAA GGTCTCCATG AGAAGGTTCA ATCTGCTCTG
    TTAACTCTAC AGCGGCATGG ATGTCTCCTC CGAGATCTTG TCCGAGTTCG AGACCGGGAC
    GTCTTCACGG CCGTTTCACG CGCTCTCGTG GGTCAGCCGG GCTACACGTA CCGTTATCTG
    GACACTCGTC TGTTCACCAT CCCCTGGCAC TGTGAAGGAG AAGAGGGCCA GAAAGATGAG
    AAAGGCAAAC CTTGCTGTGA CTCCGACCTG AGGGACGCTT GTAAAGCATT ATGGGAGCTC
    AATCAGTTCT TTTGCTCGGA TGTAAAGCAG CAGACAAATG CAAGAGGTGT CAAGCGTACC
    CGCAGTGACA CTGAAAACAG CGAAGATGCG CCAGGGGAGG GAATGTGCGA AGAAGAGAGT
    GTTAAGGACA GGTTAGTTGA GGAGAAAACC ATAGAGGAGG AGGAGGAAGA CAGTGGACAG
    GGCTGCTCTC ATTCCTCACC TCCTTCATCC ACTCCGCCAG CTGCTCAGGC CAGCCTGGTT
    CAGTTTAACG TCACGCTAAT CAACTACATG AACCCTGCAG CCATGACCCA GCTGAAGGAA
    GAGCCATATT ACGGCATGGG CAAAATGGCA GTCGGTTGGC ATCATGATGA GAACCTGGTT
    CCTCTCTCAC CAGTTGCCGT TTACAGCTAT AGCTGCCCAG CAGAGCCAAA GAATGAAGGA
    GTTACTGAGA AAGACGGGGA GGGAAAGAGC AAAGAAAAAG AAAGAGAGGC CAAAGGTGAA
    GGAACAAGTA CAGAAGAAGT GAAGAAAGAA GGAGAAGCCT CGGTGAAGGA GGACGTGGAG
    AAAGAGAAGA CGTGTTGGCG AGTTGGTTTG AAGGTGGCCT GGGACATCCA CACACCAGGT
    CTGGCTTTGC CTCTCCAATC TGGAGACTGC TACTATATGA CAGATGATCT GAACCGGACA
    CATCAGCACT GTGTACTGGC TGGTGACACA GCTAGATTCA GCTCAACTCA CAGAGTCGCA
    CAGTGTTGTA CGGGTACACT GGACTACATA CAGAAACGTT GCTCTGAGGC CTTGGAGAAT
    TTACACAGCG ACCCCGAGAA GAACGCCAAG AGCCTGATTT CCCTCCTGCC CTCCATCCTC
    CAACGCATTG AGGATATCCA CAACGAGGTG GAGTTTGAGT GGCTGAGGCA GTACTGGTTC
    CAGGGCCGGC GTTACGCTCG ATTCTGCAGC TGGTGGACTA AACCCATGGA GCAGATGGAG
    AAAGATTGGA AGGAAATGGA GAGAATGACT CAGCTGCTGT TGGTGGTGGT TGAGGATGAG
    GCCACGGCAC AGGAGAACAG AAGAGAGATG GCAGACGTGT TACTAAATGC GCTGACCGAC
    AGACAGCAGC ACAGACAGAC ATGGAGAGAC AGATGCCAGT CCAGCCTGGC GCAGACTTTG
    CCCCCTGAGG AAGCACCTGT GGACAGACCC TATTGGAGCA ATGATGATCC TGACATGCCC
    CTCCCCTTTG ACCTCTCTGA CATCATCAAC CGCGTTGAGT CGCTTCTATG GAGAATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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