vps-54 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	vps-54
Entrez Gene ID	179473
Full Name	Vacuolar protein sorting-associated protein 54
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001269298.1	NP_001256227.1	Vacuolar protein sorting-associated protein 54
NM_001269297.1	NP_001256226.1	Vacuolar protein sorting-associated protein 54
NM_001269297.2	NP_001256226.1	Vacuolar protein sorting-associated protein 54
NM_001269298.2	NP_001256227.1	Vacuolar protein sorting-associated protein 54

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS54	NP_057600.2
Canis lupus familiaris (dog)	VPS54	XP_865972.2
	VPS54	XP_001087667.1
Bos taurus (cattle)	VPS54	NP_001098930.1
Mus musculus (house mouse)	Vps54	NP_620692.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008353	XP_317102.3
Pan troglodytes (chimpanzee)	VPS54	XP_515511.2
Rattus norvegicus (Norway rat)	Vps54	NP_775170.1
Gallus gallus (chicken)	VPS54	XP_419349.3
Oryza sativa (rice)	Os04g0212100	NP_001173796.1
Caenorhabditis elegans (roundworm)	vps-54	NP_001256226.1
Xenopus tropicalis (tropical clawed frog)	vps54	XP_004914765.1
Danio rerio (zebrafish)	vps54	NP_001124248.1
Drosophila melanogaster (fruit fly)	scat	NP_524905.1
Arabidopsis thaliana (thale cress)	VPS54	NP_849409.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vps-54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps-54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa346448	NM_001269298.2 Latest version!	Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa344963	NM_001269297.2 Latest version!	Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa344963	NM_001269297.1	Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCa346448	NM_001269298.1	Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps-54 ( NM_001269298.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256227.1 Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), partial mRNA.

Price & Availability↑

CloneID	OCa346448
Clone ID Related Accession (Same CDS sequence)	NM_001269298.1 , NM_001269298.2
Accession Version	NM_001269298.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Vacuolar protein sorting-associated protein 54
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGATGCATG ACGTTGTGGA TGGAAGATTG GCTGGAAAAT TAGTTGCAAA AACTGATGAT 
TTTTGGCAAG TCGTCCGATC GTACAGTGGC CTCCAGGAAC AACTTGCCAA CGCGTTGCAA 
TGTGTGATGG TTGTTCGAAA AAACTTGAAA AGAGTCGACG AACTAGTATG CGACCAGTCA 
AAAAAGATTG TAGAAGTTCA TGAAAGATAC GAACAAAAAA AGAAACTGCT GGCAAAACTT 
CATGATATTT CATGTCTCCG AGAAGCCCAA TCAACAGTTC AAATGATGTT ATCACAAGGA 
GATTACCCAA AGGCTATAGA ATGCATTGAA ACGAGTCTAG ATGTTCTCTC AAAGGAATTG 
AACGGCGTCA CTTGTTTCCG ACATCTTGCA TCACAGCTTC GAGAGCTTTA TACAGTAATT 
GGACGTATGA TGAATGAAGA TTTTGCATCA TTAATACAAA AAGAGCTCGG TGTGAAGCCA 
GAAGCTGGAA CGATGATTCA AGCCGAAGGA GAACTTTCTG CTGTCCTACT TGGATTGATG 
AGAATGCGAA AGTACTCATT TATTGCAGTT TTAAGAGAGG AAATTATTGA AGGAGTCAAA 
AGTGTAATGC GACAAGTTAT TAAAAATCAA ATTCTAAATA ATGGAGTTGA TTTGAACGAT 
TTTGATCCTA GTTTGACACA GTTGGGAGAG CCAGTACGAA GAATGAAACA TGCTGATTTT 
TTGAAAACTG TGAAAGCTGT TATGGATGAA GAGTTCTACT TTTGTAAACG ATTGGAAGCT 
CTACAAGATA TTCTACTGGA AACTGTTGAA CGAGCAAATC CTCTACATCG ACATGGCTCT 
GAAGATATTA TAGTTGAGAG AATCGAAGAA GCAAAAGATA TTCACGAATC TGAGTCTGAC 
GATGAAGTTG GTACAACTTT TTCCAAATCA GCTAGTTCAG GAGGATTCTC CGTCGGTGGT 
TCGGCTTTAT CCTCTAACGC GTCAGCTACC ACTCTTCTTT CGATTGAAGT CAGATCAGAA 
GCCTTTTTAC GACACGTGCT CCCATTGATC GCAGAATTCG GTCATCAATG TGCGCAACAA 
CGGATTTCAC GATTGCTCAT TGCTCGGGCC AAAAATGCAT CAGTGACAGA AGCTACTACA 
CCTACCCAAC TATCTGAATG CATTAAACTC GTTAAAGAAT TTCAATCCAA GTGTGACAAG 
GAAGGATGGT ATTCCACTCA GAATCAAAAA GTTGGAGGTC TTGGAAGATC TGTTAACAAG 
CTTTCTATGG ATTACATTGA AAAATTCCAT GCAGCTCGAA AGATTCGTAT TGGAAATATG 
CTTGACACAG AACTTTGGAA AGCGACTGAT GTTTCGATTG TGGATCAGAA CATTGTCGAC 
ATGGCGATGG AAACGGGCCA ATTAAGAAAC ACAAAGCGAA TAGATGATGG TCCAATAAAA 
AAATCATTCA AAAGAACAGA AAGCGCTGTG ACAATTGATT CAACAACATC TACTTCAAAT 
CAAATTCAAG GTGTAATTGT TGATGAAGAG AACTATGTTG TTGTTGGATC ATCAATAACA 
ATGATCCAGC TCCTTTCTGA CTACTGTGAA GCCATATCTG AAATGCCATC ATTTTCCCAA 
GACTGGAATT CGCGAGTTGT GGAATTACTG AAAACATTTA ACAGTCGGTG TTGTCAATTG 
ATTTTAGGTG CAGGTGCCCT ACAATTGGTT GGATTAAAGA CTATATCTGT TCGAAATTTA 
GCACTCGCAG GTCGATCACT CGAGTTAGTA TGCCGCTTCA TTCCGATGGT TCATGATGAG 
ATGGACAGAG TTCTTCCTGA AAATCGAAAA AGTCTGCTTC GGTACTTCAA GCAGGTTGAA 
AGTGAATACC GCGATCACGT CAACGAAATT GCTGCCAAAT TAATAAGTGT AATAGCTCAT 
TATACAACAA ATTGTCTTGG AATGTGGGAT GTGAAAGGAG TGATTCCATC GCCAGAATTT 
CAACAAATTT GTCGCCATAT GCTGAAATTC CACAATGGAC TGGTTGGCAT CATGCCAAGA 
GATCAGATCG AGGCGTTGTT CCGTCAAGTA CACGAAAACT TTAAAGCCAA CTTACGAGAA 
CACGTCACTG GAATGGGTAT CACACCTCAT GACCCTTTAA AATACGGATA TGTCACAAAA 
GATTACATGT TTTATCAACA AAATGTGAAA AACATGGAAA GTTGCCGTAA TTTGGAGCTC 
GAGTCGCTTA ATGACATTAT GTTTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256227.1
CDS	1..2250
Translation	MMHDVVDGRLAGKLVAKTDDFWQVVRSYSGLQEQLANALQCVMVVRKNLKRVDELVCDQSKKIVEVHERYEQKKKLLAKLHDISCLREAQSTVQMMLSQGDYPKAIECIETSLDVLSKELNGVTCFRHLASQLRELYTVIGRMMNEDFASLIQKELGVKPEAGTMIQAEGELSAVLLGLMRMRKYSFIAVLREEIIEGVKSVMRQVIKNQILNNGVDLNDFDPSLTQLGEPVRRMKHADFLKTVKAVMDEEFYFCKRLEALQDILLETVERANPLHRHGSEDIIVERIEEAKDIHESESDDEVGTTFSKSASSGGFSVGGSALSSNASATTLLSIEVRSEAFLRHVLPLIAEFGHQCAQQRISRLLIARAKNASVTEATTPTQLSECIKLVKEFQSKCDKEGWYSTQNQKVGGLGRSVNKLSMDYIEKFHAARKIRIGNMLDTELWKATDVSIVDQNIVDMAMETGQLRNTKRIDDGPIKKSFKRTESAVTIDSTTSTSNQIQGVIVDEENYVVVGSSITMIQLLSDYCEAISEMPSFSQDWNSRVVELLKTFNSRCCQLILGAGALQLVGLKTISVRNLALAGRSLELVCRFIPMVHDEMDRVLPENRKSLLRYFKQVESEYRDHVNEIAAKLISVIAHYTTNCLGMWDVKGVIPSPEFQQICRHMLKFHNGLVGIMPRDQIEALFRQVHENFKANLREHVTGMGITPHDPLKYGYVTKDYMFYQQNVKNMESCRNLELESLNDIMFE

Target ORF information:

RefSeq Version	NM_001269298.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269298.1

ORF Insert Sequence:

ATGATGCATG ACGTTGTGGA TGGAAGATTG GCTGGAAAAT TAGTTGCAAA AACTGATGAT 
TTTTGGCAAG TCGTCCGATC GTACAGTGGC CTCCAGGAAC AACTTGCCAA CGCGTTGCAA 
TGTGTGATGG TTGTTCGAAA AAACTTGAAA AGAGTCGACG AACTAGTATG CGACCAGTCA 
AAAAAGATTG TAGAAGTTCA TGAAAGATAC GAACAAAAAA AGAAACTGCT GGCAAAACTT 
CATGATATTT CATGTCTCCG AGAAGCCCAA TCAACAGTTC AAATGATGTT ATCACAAGGA 
GATTACCCAA AGGCTATAGA ATGCATTGAA ACGAGTCTAG ATGTTCTCTC AAAGGAATTG 
AACGGCGTCA CTTGTTTCCG ACATCTTGCA TCACAGCTTC GAGAGCTTTA TACAGTAATT 
GGACGTATGA TGAATGAAGA TTTTGCATCA TTAATACAAA AAGAGCTCGG TGTGAAGCCA 
GAAGCTGGAA CGATGATTCA AGCCGAAGGA GAACTTTCTG CTGTCCTACT TGGATTGATG 
AGAATGCGAA AGTACTCATT TATTGCAGTT TTAAGAGAGG AAATTATTGA AGGAGTCAAA 
AGTGTAATGC GACAAGTTAT TAAAAATCAA ATTCTAAATA ATGGAGTTGA TTTGAACGAT 
TTTGATCCTA GTTTGACACA GTTGGGAGAG CCAGTACGAA GAATGAAACA TGCTGATTTT 
TTGAAAACTG TGAAAGCTGT TATGGATGAA GAGTTCTACT TTTGTAAACG ATTGGAAGCT 
CTACAAGATA TTCTACTGGA AACTGTTGAA CGAGCAAATC CTCTACATCG ACATGGCTCT 
GAAGATATTA TAGTTGAGAG AATCGAAGAA GCAAAAGATA TTCACGAATC TGAGTCTGAC 
GATGAAGTTG GTACAACTTT TTCCAAATCA GCTAGTTCAG GAGGATTCTC CGTCGGTGGT 
TCGGCTTTAT CCTCTAACGC GTCAGCTACC ACTCTTCTTT CGATTGAAGT CAGATCAGAA 
GCCTTTTTAC GACACGTGCT CCCATTGATC GCAGAATTCG GTCATCAATG TGCGCAACAA 
CGGATTTCAC GATTGCTCAT TGCTCGGGCC AAAAATGCAT CAGTGACAGA AGCTACTACA 
CCTACCCAAC TATCTGAATG CATTAAACTC GTTAAAGAAT TTCAATCCAA GTGTGACAAG 
GAAGGATGGT ATTCCACTCA GAATCAAAAA GTTGGAGGTC TTGGAAGATC TGTTAACAAG 
CTTTCTATGG ATTACATTGA AAAATTCCAT GCAGCTCGAA AGATTCGTAT TGGAAATATG 
CTTGACACAG AACTTTGGAA AGCGACTGAT GTTTCGATTG TGGATCAGAA CATTGTCGAC 
ATGGCGATGG AAACGGGCCA ATTAAGAAAC ACAAAGCGAA TAGATGATGG TCCAATAAAA 
AAATCATTCA AAAGAACAGA AAGCGCTGTG ACAATTGATT CAACAACATC TACTTCAAAT 
CAAATTCAAG GTGTAATTGT TGATGAAGAG AACTATGTTG TTGTTGGATC ATCAATAACA 
ATGATCCAGC TCCTTTCTGA CTACTGTGAA GCCATATCTG AAATGCCATC ATTTTCCCAA 
GACTGGAATT CGCGAGTTGT GGAATTACTG AAAACATTTA ACAGTCGGTG TTGTCAATTG 
ATTTTAGGTG CAGGTGCCCT ACAATTGGTT GGATTAAAGA CTATATCTGT TCGAAATTTA 
GCACTCGCAG GTCGATCACT CGAGTTAGTA TGCCGCTTCA TTCCGATGGT TCATGATGAG 
ATGGACAGAG TTCTTCCTGA AAATCGAAAA AGTCTGCTTC GGTACTTCAA GCAGGTTGAA 
AGTGAATACC GCGATCACGT CAACGAAATT GCTGCCAAAT TAATAAGTGT AATAGCTCAT 
TATACAACAA ATTGTCTTGG AATGTGGGAT GTGAAAGGAG TGATTCCATC GCCAGAATTT 
CAACAAATTT GTCGCCATAT GCTGAAATTC CACAATGGAC TGGTTGGCAT CATGCCAAGA 
GATCAGATCG AGGCGTTGTT CCGTCAAGTA CACGAAAACT TTAAAGCCAA CTTACGAGAA 
CACGTCACTG GAATGGGTAT CACACCTCAT GACCCTTTAA AATACGGATA TGTCACAAAA 
GATTACATGT TTTATCAACA AAATGTGAAA AACATGGAAA GTTGCCGTAA TTTGGAGCTC 
GAGTCGCTTA ATGACATTAT GTTTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps-54 ( NM_001269298.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256227.1 Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), mRNA.

Price & Availability↑

CloneID	OCa346448
Clone ID Related Accession (Same CDS sequence)	NM_001269298.1 , NM_001269298.2
Accession Version	NM_001269298.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Vacuolar protein sorting-associated protein 54
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269298.1.

ATGATGCATG ACGTTGTGGA TGGAAGATTG GCTGGAAAAT TAGTTGCAAA AACTGATGAT 
TTTTGGCAAG TCGTCCGATC GTACAGTGGC CTCCAGGAAC AACTTGCCAA CGCGTTGCAA 
TGTGTGATGG TTGTTCGAAA AAACTTGAAA AGAGTCGACG AACTAGTATG CGACCAGTCA 
AAAAAGATTG TAGAAGTTCA TGAAAGATAC GAACAAAAAA AGAAACTGCT GGCAAAACTT 
CATGATATTT CATGTCTCCG AGAAGCCCAA TCAACAGTTC AAATGATGTT ATCACAAGGA 
GATTACCCAA AGGCTATAGA ATGCATTGAA ACGAGTCTAG ATGTTCTCTC AAAGGAATTG 
AACGGCGTCA CTTGTTTCCG ACATCTTGCA TCACAGCTTC GAGAGCTTTA TACAGTAATT 
GGACGTATGA TGAATGAAGA TTTTGCATCA TTAATACAAA AAGAGCTCGG TGTGAAGCCA 
GAAGCTGGAA CGATGATTCA AGCCGAAGGA GAACTTTCTG CTGTCCTACT TGGATTGATG 
AGAATGCGAA AGTACTCATT TATTGCAGTT TTAAGAGAGG AAATTATTGA AGGAGTCAAA 
AGTGTAATGC GACAAGTTAT TAAAAATCAA ATTCTAAATA ATGGAGTTGA TTTGAACGAT 
TTTGATCCTA GTTTGACACA GTTGGGAGAG CCAGTACGAA GAATGAAACA TGCTGATTTT 
TTGAAAACTG TGAAAGCTGT TATGGATGAA GAGTTCTACT TTTGTAAACG ATTGGAAGCT 
CTACAAGATA TTCTACTGGA AACTGTTGAA CGAGCAAATC CTCTACATCG ACATGGCTCT 
GAAGATATTA TAGTTGAGAG AATCGAAGAA GCAAAAGATA TTCACGAATC TGAGTCTGAC 
GATGAAGTTG GTACAACTTT TTCCAAATCA GCTAGTTCAG GAGGATTCTC CGTCGGTGGT 
TCGGCTTTAT CCTCTAACGC GTCAGCTACC ACTCTTCTTT CGATTGAAGT CAGATCAGAA 
GCCTTTTTAC GACACGTGCT CCCATTGATC GCAGAATTCG GTCATCAATG TGCGCAACAA 
CGGATTTCAC GATTGCTCAT TGCTCGGGCC AAAAATGCAT CAGTGACAGA AGCTACTACA 
CCTACCCAAC TATCTGAATG CATTAAACTC GTTAAAGAAT TTCAATCCAA GTGTGACAAG 
GAAGGATGGT ATTCCACTCA GAATCAAAAA GTTGGAGGTC TTGGAAGATC TGTTAACAAG 
CTTTCTATGG ATTACATTGA AAAATTCCAT GCAGCTCGAA AGATTCGTAT TGGAAATATG 
CTTGACACAG AACTTTGGAA AGCGACTGAT GTTTCGATTG TGGATCAGAA CATTGTCGAC 
ATGGCGATGG AAACGGGCCA ATTAAGAAAC ACAAAGCGAA TAGATGATGG TCCAATAAAA 
AAATCATTCA AAAGAACAGA AAGCGCTGTG ACAATTGATT CAACAACATC TACTTCAAAT 
CAAATTCAAG GTGTAATTGT TGATGAAGAG AACTATGTTG TTGTTGGATC ATCAATAACA 
ATGATCCAGC TCCTTTCTGA CTACTGTGAA GCCATATCTG AAATGCCATC ATTTTCCCAA 
GACTGGAATT CGCGAGTTGT GGAATTACTG AAAACATTTA ACAGTCGGTG TTGTCAATTG 
ATTTTAGGTG CAGGTGCCCT ACAATTGGTT GGATTAAAGA CTATATCTGT TCGAAATTTA 
GCACTCGCAG GTCGATCACT CGAGTTAGTA TGCCGCTTCA TTCCGATGGT TCATGATGAG 
ATGGACAGAG TTCTTCCTGA AAATCGAAAA AGTCTGCTTC GGTACTTCAA GCAGGTTGAA 
AGTGAATACC GCGATCACGT CAACGAAATT GCTGCCAAAT TAATAAGTGT AATAGCTCAT 
TATACAACAA ATTGTCTTGG AATGTGGGAT GTGAAAGGAG TGATTCCATC GCCAGAATTT 
CAACAAATTT GTCGCCATAT GCTGAAATTC CACAATGGAC TGGTTGGCAT CATGCCAAGA 
GATCAGATCG AGGCGTTGTT CCGTCAAGTA CACGAAAACT TTAAAGCCAA CTTACGAGAA 
CACGTCACTG GAATGGGTAT CACACCTCAT GACCCTTTAA AATACGGATA TGTCACAAAA 
GATTACATGT TTTATCAACA AAATGTGAAA AACATGGAAA GTTGCCGTAA TTTGGAGCTC 
GAGTCGCTTA ATGACATTAT GTTTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256227.1
CDS	4..2253
Translation	MMHDVVDGRLAGKLVAKTDDFWQVVRSYSGLQEQLANALQCVMVVRKNLKRVDELVCDQSKKIVEVHERYEQKKKLLAKLHDISCLREAQSTVQMMLSQGDYPKAIECIETSLDVLSKELNGVTCFRHLASQLRELYTVIGRMMNEDFASLIQKELGVKPEAGTMIQAEGELSAVLLGLMRMRKYSFIAVLREEIIEGVKSVMRQVIKNQILNNGVDLNDFDPSLTQLGEPVRRMKHADFLKTVKAVMDEEFYFCKRLEALQDILLETVERANPLHRHGSEDIIVERIEEAKDIHESESDDEVGTTFSKSASSGGFSVGGSALSSNASATTLLSIEVRSEAFLRHVLPLIAEFGHQCAQQRISRLLIARAKNASVTEATTPTQLSECIKLVKEFQSKCDKEGWYSTQNQKVGGLGRSVNKLSMDYIEKFHAARKIRIGNMLDTELWKATDVSIVDQNIVDMAMETGQLRNTKRIDDGPIKKSFKRTESAVTIDSTTSTSNQIQGVIVDEENYVVVGSSITMIQLLSDYCEAISEMPSFSQDWNSRVVELLKTFNSRCCQLILGAGALQLVGLKTISVRNLALAGRSLELVCRFIPMVHDEMDRVLPENRKSLLRYFKQVESEYRDHVNEIAAKLISVIAHYTTNCLGMWDVKGVIPSPEFQQICRHMLKFHNGLVGIMPRDQIEALFRQVHENFKANLREHVTGMGITPHDPLKYGYVTKDYMFYQQNVKNMESCRNLELESLNDIMFE

Target ORF information:

RefSeq Version	NM_001269298.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269298.2

ORF Insert Sequence:

ATGATGCATG ACGTTGTGGA TGGAAGATTG GCTGGAAAAT TAGTTGCAAA AACTGATGAT 
TTTTGGCAAG TCGTCCGATC GTACAGTGGC CTCCAGGAAC AACTTGCCAA CGCGTTGCAA 
TGTGTGATGG TTGTTCGAAA AAACTTGAAA AGAGTCGACG AACTAGTATG CGACCAGTCA 
AAAAAGATTG TAGAAGTTCA TGAAAGATAC GAACAAAAAA AGAAACTGCT GGCAAAACTT 
CATGATATTT CATGTCTCCG AGAAGCCCAA TCAACAGTTC AAATGATGTT ATCACAAGGA 
GATTACCCAA AGGCTATAGA ATGCATTGAA ACGAGTCTAG ATGTTCTCTC AAAGGAATTG 
AACGGCGTCA CTTGTTTCCG ACATCTTGCA TCACAGCTTC GAGAGCTTTA TACAGTAATT 
GGACGTATGA TGAATGAAGA TTTTGCATCA TTAATACAAA AAGAGCTCGG TGTGAAGCCA 
GAAGCTGGAA CGATGATTCA AGCCGAAGGA GAACTTTCTG CTGTCCTACT TGGATTGATG 
AGAATGCGAA AGTACTCATT TATTGCAGTT TTAAGAGAGG AAATTATTGA AGGAGTCAAA 
AGTGTAATGC GACAAGTTAT TAAAAATCAA ATTCTAAATA ATGGAGTTGA TTTGAACGAT 
TTTGATCCTA GTTTGACACA GTTGGGAGAG CCAGTACGAA GAATGAAACA TGCTGATTTT 
TTGAAAACTG TGAAAGCTGT TATGGATGAA GAGTTCTACT TTTGTAAACG ATTGGAAGCT 
CTACAAGATA TTCTACTGGA AACTGTTGAA CGAGCAAATC CTCTACATCG ACATGGCTCT 
GAAGATATTA TAGTTGAGAG AATCGAAGAA GCAAAAGATA TTCACGAATC TGAGTCTGAC 
GATGAAGTTG GTACAACTTT TTCCAAATCA GCTAGTTCAG GAGGATTCTC CGTCGGTGGT 
TCGGCTTTAT CCTCTAACGC GTCAGCTACC ACTCTTCTTT CGATTGAAGT CAGATCAGAA 
GCCTTTTTAC GACACGTGCT CCCATTGATC GCAGAATTCG GTCATCAATG TGCGCAACAA 
CGGATTTCAC GATTGCTCAT TGCTCGGGCC AAAAATGCAT CAGTGACAGA AGCTACTACA 
CCTACCCAAC TATCTGAATG CATTAAACTC GTTAAAGAAT TTCAATCCAA GTGTGACAAG 
GAAGGATGGT ATTCCACTCA GAATCAAAAA GTTGGAGGTC TTGGAAGATC TGTTAACAAG 
CTTTCTATGG ATTACATTGA AAAATTCCAT GCAGCTCGAA AGATTCGTAT TGGAAATATG 
CTTGACACAG AACTTTGGAA AGCGACTGAT GTTTCGATTG TGGATCAGAA CATTGTCGAC 
ATGGCGATGG AAACGGGCCA ATTAAGAAAC ACAAAGCGAA TAGATGATGG TCCAATAAAA 
AAATCATTCA AAAGAACAGA AAGCGCTGTG ACAATTGATT CAACAACATC TACTTCAAAT 
CAAATTCAAG GTGTAATTGT TGATGAAGAG AACTATGTTG TTGTTGGATC ATCAATAACA 
ATGATCCAGC TCCTTTCTGA CTACTGTGAA GCCATATCTG AAATGCCATC ATTTTCCCAA 
GACTGGAATT CGCGAGTTGT GGAATTACTG AAAACATTTA ACAGTCGGTG TTGTCAATTG 
ATTTTAGGTG CAGGTGCCCT ACAATTGGTT GGATTAAAGA CTATATCTGT TCGAAATTTA 
GCACTCGCAG GTCGATCACT CGAGTTAGTA TGCCGCTTCA TTCCGATGGT TCATGATGAG 
ATGGACAGAG TTCTTCCTGA AAATCGAAAA AGTCTGCTTC GGTACTTCAA GCAGGTTGAA 
AGTGAATACC GCGATCACGT CAACGAAATT GCTGCCAAAT TAATAAGTGT AATAGCTCAT 
TATACAACAA ATTGTCTTGG AATGTGGGAT GTGAAAGGAG TGATTCCATC GCCAGAATTT 
CAACAAATTT GTCGCCATAT GCTGAAATTC CACAATGGAC TGGTTGGCAT CATGCCAAGA 
GATCAGATCG AGGCGTTGTT CCGTCAAGTA CACGAAAACT TTAAAGCCAA CTTACGAGAA 
CACGTCACTG GAATGGGTAT CACACCTCAT GACCCTTTAA AATACGGATA TGTCACAAAA 
GATTACATGT TTTATCAACA AAATGTGAAA AACATGGAAA GTTGCCGTAA TTTGGAGCTC 
GAGTCGCTTA ATGACATTAT GTTTGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps-54 ( NM_001269297.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256226.1 Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), partial mRNA.

Price & Availability↑

CloneID	OCa344963
Clone ID Related Accession (Same CDS sequence)	NM_001269297.1 , NM_001269297.2
Accession Version	NM_001269297.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Vacuolar protein sorting-associated protein 54
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGAAATCAA TGGATGACCG AACAGTAGGT CTTATATCTG CAAGTTCATC AAGAGCTTCA 
CTCAAAATTC AACCGGAGAT TGGTTATCCA AGAATATGTG ATTATTGTCA GCCAATTATC 
GAGCTTATGA CGGCTTCCGA GTTTGCACGA CATATTCGAC AAGATCATAC GACAAAAGAA 
GGTGGTAGCT TTCTTTGTAG ATACGGAGAA CATGGAGTAT GTCAGAAACT ACCACTGGAA 
GGAGTATGTG ATCTTGATTT TGAAGCCCAC ATTCGTCGTT GTCACACTTC TTCTCAACCA 
GCTGGATATT CAAGTCCAAC ATCTTCATTG GTAGCAAGTT ATACTGAAGA TAGTGAAGAA 
ACAGCTAGTT TGAGAAGTAT ACGATTGACA AGTGATCGAG ATACTCCAAC AATTGAAAAG 
AAAAAATTCA CTCTTCACAG TTTCACTCAA AATCTGTCAG CAGTTCTTGC CGATCCATCA 
CGATCTCGAA GTGACCTAAC AACGTTCTTC ACACGCCATT GGGGTGACAG TTTTGTTCCA 
ACACAACCGG TACCACAATC TAAAAGACTC GCTCGGCTCG CTGATTCGGC TTTCGATAGT 
TATTGTCAAT CTGCTGGAGA GTCCTATCGT CGATATCAGG CAATCAAAAG AGCTCTACGT 
TTGAGTCACG CCGATGGAGG AGTTGGAATT GGAGATGAAA GACAAGATGC GGAAGATCTG 
CCTACGATAT TCATCGACCC CCGATTTACA CTTGGCGACA GTTCTACTTT TTCGGATGTG 
TTCACAGTTC CTGCCAATGA TGATTTGGAT GCATTGAAAC AAACTTTATC AGGTAGAAAT 
GTGATTCCAG CTACACCTTT AGAAATTGCT ACCAACAGGA AACCTGGAGA ATTTCGAGAC 
TACGAAGCTC TTCAAAACAG ATTGGAAATG ATGCATGACG TTGTGGATGG AAGATTGGCT 
GGAAAATTAG TTGCAAAAAC TGATGATTTT TGGCAAGTCG TCCGATCGTA CAGTGGCCTC 
CAGGAACAAC TTGCCAACGC GTTGCAATGT GTGATGGTTG TTCGAAAAAA CTTGAAAAGA 
GTCGACGAAC TAGTATGCGA CCAGTCAAAA AAGATTGTAG AAGTTCATGA AAGATACGAA 
CAAAAAAAGA AACTGCTGGC AAAACTTCAT GATATTTCAT GTCTCCGAGA AGCCCAATCA 
ACAGTTCAAA TGATGTTATC ACAAGGAGAT TACCCAAAGG CTATAGAATG CATTGAAACG 
AGTCTAGATG TTCTCTCAAA GGAATTGAAC GGCGTCACTT GTTTCCGACA TCTTGCATCA 
CAGCTTCGAG AGCTTTATAC AGTAATTGGA CGTATGATGA ATGAAGATTT TGCATCATTA 
ATACAAAAAG AGCTCGGTGT GAAGCCAGAA GCTGGAACGA TGATTCAAGC CGAAGGAGAA 
CTTTCTGCTG TCCTACTTGG ATTGATGAGA ATGCGAAAGT ACTCATTTAT TGCAGTTTTA 
AGAGAGGAAA TTATTGAAGG AGTCAAAAGT GTAATGCGAC AAGTTATTAA AAATCAAATT 
CTAAATAATG GAGTTGATTT GAACGATTTT GATCCTAGTT TGACACAGTT GGGAGAGCCA 
GTACGAAGAA TGAAACATGC TGATTTTTTG AAAACTGTGA AAGCTGTTAT GGATGAAGAG 
TTCTACTTTT GTAAACGATT GGAAGCTCTA CAAGATATTC TACTGGAAAC TGTTGAACGA 
GCAAATCCTC TACATCGACA TGGCTCTGAA GATATTATAG TTGAGAGAAT CGAAGAAGCA 
AAAGATATTC ACGAATCTGA GTCTGACGAT GAAGTTGGTA CAACTTTTTC CAAATCAGCT 
AGTTCAGGAG GATTCTCCGT CGGTGGTTCG GCTTTATCCT CTAACGCGTC AGCTACCACT 
CTTCTTTCGA TTGAAGTCAG ATCAGAAGCC TTTTTACGAC ACGTGCTCCC ATTGATCGCA 
GAATTCGGTC ATCAATGTGC GCAACAACGG ATTTCACGAT TGCTCATTGC TCGGGCCAAA 
AATGCATCAG TGACAGAAGC TACTACACCT ACCCAACTAT CTGAATGCAT TAAACTCGTT 
AAAGAATTTC AATCCAAGTG TGACAAGGAA GGATGGTATT CCACTCAGAA TCAAAAAGTT 
GGAGGTCTTG GAAGATCTGT TAACAAGCTT TCTATGGATT ACATTGAAAA ATTCCATGCA 
GCTCGAAAGA TTCGTATTGG AAATATGCTT GACACAGAAC TTTGGAAAGC GACTGATGTT 
TCGATTGTGG ATCAGAACAT TGTCGACATG GCGATGGAAA CGGGCCAATT AAGAAACACA 
AAGCGAATAG ATGATGGTCC AATAAAAAAA TCATTCAAAA GAACAGAAAG CGCTGTGACA 
ATTGATTCAA CAACATCTAC TTCAAATCAA ATTCAAGGTG TAATTGTTGA TGAAGAGAAC 
TATGTTGTTG TTGGATCATC AATAACAATG ATCCAGCTCC TTTCTGACTA CTGTGAAGCC 
ATATCTGAAA TGCCATCATT TTCCCAAGAC TGGAATTCGC GAGTTGTGGA ATTACTGAAA 
ACATTTAACA GTCGGTGTTG TCAATTGATT TTAGGTGCAG GTGCCCTACA ATTGGTTGGA 
TTAAAGACTA TATCTGTTCG AAATTTAGCA CTCGCAGGTC GATCACTCGA GTTAGTATGC 
CGCTTCATTC CGATGGTTCA TGATGAGATG GACAGAGTTC TTCCTGAAAA TCGAAAAAGT 
CTGCTTCGGT ACTTCAAGCA GGTTGAAAGT GAATACCGCG ATCACGTCAA CGAAATTGCT 
GCCAAATTAA TAAGTGTAAT AGCTCATTAT ACAACAAATT GTCTTGGAAT GTGGGATGTG 
AAAGGAGTGA TTCCATCGCC AGAATTTCAA CAAATTTGTC GCCATATGCT GAAATTCCAC 
AATGGACTGG TTGGCATCAT GCCAAGAGAT CAGATCGAGG CGTTGTTCCG TCAAGTACAC 
GAAAACTTTA AAGCCAACTT ACGAGAACAC GTCACTGGAA TGGGTATCAC ACCTCATGAC 
CCTTTAAAAT ACGGATATGT CACAAAAGAT TACATGTTTT ATCAACAAAA TGTGAAAAAC 
ATGGAAAGTT GCCGTAATTT GGAGCTCGAG TCGCTTAATG ACATTATGTT TGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256226.1
CDS	1..3177
Translation	MKSMDDRTVGLISASSSRASLKIQPEIGYPRICDYCQPIIELMTASEFARHIRQDHTTKEGGSFLCRYGEHGVCQKLPLEGVCDLDFEAHIRRCHTSSQPAGYSSPTSSLVASYTEDSEETASLRSIRLTSDRDTPTIEKKKFTLHSFTQNLSAVLADPSRSRSDLTTFFTRHWGDSFVPTQPVPQSKRLARLADSAFDSYCQSAGESYRRYQAIKRALRLSHADGGVGIGDERQDAEDLPTIFIDPRFTLGDSSTFSDVFTVPANDDLDALKQTLSGRNVIPATPLEIATNRKPGEFRDYEALQNRLEMMHDVVDGRLAGKLVAKTDDFWQVVRSYSGLQEQLANALQCVMVVRKNLKRVDELVCDQSKKIVEVHERYEQKKKLLAKLHDISCLREAQSTVQMMLSQGDYPKAIECIETSLDVLSKELNGVTCFRHLASQLRELYTVIGRMMNEDFASLIQKELGVKPEAGTMIQAEGELSAVLLGLMRMRKYSFIAVLREEIIEGVKSVMRQVIKNQILNNGVDLNDFDPSLTQLGEPVRRMKHADFLKTVKAVMDEEFYFCKRLEALQDILLETVERANPLHRHGSEDIIVERIEEAKDIHESESDDEVGTTFSKSASSGGFSVGGSALSSNASATTLLSIEVRSEAFLRHVLPLIAEFGHQCAQQRISRLLIARAKNASVTEATTPTQLSECIKLVKEFQSKCDKEGWYSTQNQKVGGLGRSVNKLSMDYIEKFHAARKIRIGNMLDTELWKATDVSIVDQNIVDMAMETGQLRNTKRIDDGPIKKSFKRTESAVTIDSTTSTSNQIQGVIVDEENYVVVGSSITMIQLLSDYCEAISEMPSFSQDWNSRVVELLKTFNSRCCQLILGAGALQLVGLKTISVRNLALAGRSLELVCRFIPMVHDEMDRVLPENRKSLLRYFKQVESEYRDHVNEIAAKLISVIAHYTTNCLGMWDVKGVIPSPEFQQICRHMLKFHNGLVGIMPRDQIEALFRQVHENFKANLREHVTGMGITPHDPLKYGYVTKDYMFYQQNVKNMESCRNLELESLNDIMFE

Target ORF information:

RefSeq Version	NM_001269297.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269297.1

ORF Insert Sequence:

ATGAAATCAA TGGATGACCG AACAGTAGGT CTTATATCTG CAAGTTCATC AAGAGCTTCA 
CTCAAAATTC AACCGGAGAT TGGTTATCCA AGAATATGTG ATTATTGTCA GCCAATTATC 
GAGCTTATGA CGGCTTCCGA GTTTGCACGA CATATTCGAC AAGATCATAC GACAAAAGAA 
GGTGGTAGCT TTCTTTGTAG ATACGGAGAA CATGGAGTAT GTCAGAAACT ACCACTGGAA 
GGAGTATGTG ATCTTGATTT TGAAGCCCAC ATTCGTCGTT GTCACACTTC TTCTCAACCA 
GCTGGATATT CAAGTCCAAC ATCTTCATTG GTAGCAAGTT ATACTGAAGA TAGTGAAGAA 
ACAGCTAGTT TGAGAAGTAT ACGATTGACA AGTGATCGAG ATACTCCAAC AATTGAAAAG 
AAAAAATTCA CTCTTCACAG TTTCACTCAA AATCTGTCAG CAGTTCTTGC CGATCCATCA 
CGATCTCGAA GTGACCTAAC AACGTTCTTC ACACGCCATT GGGGTGACAG TTTTGTTCCA 
ACACAACCGG TACCACAATC TAAAAGACTC GCTCGGCTCG CTGATTCGGC TTTCGATAGT 
TATTGTCAAT CTGCTGGAGA GTCCTATCGT CGATATCAGG CAATCAAAAG AGCTCTACGT 
TTGAGTCACG CCGATGGAGG AGTTGGAATT GGAGATGAAA GACAAGATGC GGAAGATCTG 
CCTACGATAT TCATCGACCC CCGATTTACA CTTGGCGACA GTTCTACTTT TTCGGATGTG 
TTCACAGTTC CTGCCAATGA TGATTTGGAT GCATTGAAAC AAACTTTATC AGGTAGAAAT 
GTGATTCCAG CTACACCTTT AGAAATTGCT ACCAACAGGA AACCTGGAGA ATTTCGAGAC 
TACGAAGCTC TTCAAAACAG ATTGGAAATG ATGCATGACG TTGTGGATGG AAGATTGGCT 
GGAAAATTAG TTGCAAAAAC TGATGATTTT TGGCAAGTCG TCCGATCGTA CAGTGGCCTC 
CAGGAACAAC TTGCCAACGC GTTGCAATGT GTGATGGTTG TTCGAAAAAA CTTGAAAAGA 
GTCGACGAAC TAGTATGCGA CCAGTCAAAA AAGATTGTAG AAGTTCATGA AAGATACGAA 
CAAAAAAAGA AACTGCTGGC AAAACTTCAT GATATTTCAT GTCTCCGAGA AGCCCAATCA 
ACAGTTCAAA TGATGTTATC ACAAGGAGAT TACCCAAAGG CTATAGAATG CATTGAAACG 
AGTCTAGATG TTCTCTCAAA GGAATTGAAC GGCGTCACTT GTTTCCGACA TCTTGCATCA 
CAGCTTCGAG AGCTTTATAC AGTAATTGGA CGTATGATGA ATGAAGATTT TGCATCATTA 
ATACAAAAAG AGCTCGGTGT GAAGCCAGAA GCTGGAACGA TGATTCAAGC CGAAGGAGAA 
CTTTCTGCTG TCCTACTTGG ATTGATGAGA ATGCGAAAGT ACTCATTTAT TGCAGTTTTA 
AGAGAGGAAA TTATTGAAGG AGTCAAAAGT GTAATGCGAC AAGTTATTAA AAATCAAATT 
CTAAATAATG GAGTTGATTT GAACGATTTT GATCCTAGTT TGACACAGTT GGGAGAGCCA 
GTACGAAGAA TGAAACATGC TGATTTTTTG AAAACTGTGA AAGCTGTTAT GGATGAAGAG 
TTCTACTTTT GTAAACGATT GGAAGCTCTA CAAGATATTC TACTGGAAAC TGTTGAACGA 
GCAAATCCTC TACATCGACA TGGCTCTGAA GATATTATAG TTGAGAGAAT CGAAGAAGCA 
AAAGATATTC ACGAATCTGA GTCTGACGAT GAAGTTGGTA CAACTTTTTC CAAATCAGCT 
AGTTCAGGAG GATTCTCCGT CGGTGGTTCG GCTTTATCCT CTAACGCGTC AGCTACCACT 
CTTCTTTCGA TTGAAGTCAG ATCAGAAGCC TTTTTACGAC ACGTGCTCCC ATTGATCGCA 
GAATTCGGTC ATCAATGTGC GCAACAACGG ATTTCACGAT TGCTCATTGC TCGGGCCAAA 
AATGCATCAG TGACAGAAGC TACTACACCT ACCCAACTAT CTGAATGCAT TAAACTCGTT 
AAAGAATTTC AATCCAAGTG TGACAAGGAA GGATGGTATT CCACTCAGAA TCAAAAAGTT 
GGAGGTCTTG GAAGATCTGT TAACAAGCTT TCTATGGATT ACATTGAAAA ATTCCATGCA 
GCTCGAAAGA TTCGTATTGG AAATATGCTT GACACAGAAC TTTGGAAAGC GACTGATGTT 
TCGATTGTGG ATCAGAACAT TGTCGACATG GCGATGGAAA CGGGCCAATT AAGAAACACA 
AAGCGAATAG ATGATGGTCC AATAAAAAAA TCATTCAAAA GAACAGAAAG CGCTGTGACA 
ATTGATTCAA CAACATCTAC TTCAAATCAA ATTCAAGGTG TAATTGTTGA TGAAGAGAAC 
TATGTTGTTG TTGGATCATC AATAACAATG ATCCAGCTCC TTTCTGACTA CTGTGAAGCC 
ATATCTGAAA TGCCATCATT TTCCCAAGAC TGGAATTCGC GAGTTGTGGA ATTACTGAAA 
ACATTTAACA GTCGGTGTTG TCAATTGATT TTAGGTGCAG GTGCCCTACA ATTGGTTGGA 
TTAAAGACTA TATCTGTTCG AAATTTAGCA CTCGCAGGTC GATCACTCGA GTTAGTATGC 
CGCTTCATTC CGATGGTTCA TGATGAGATG GACAGAGTTC TTCCTGAAAA TCGAAAAAGT 
CTGCTTCGGT ACTTCAAGCA GGTTGAAAGT GAATACCGCG ATCACGTCAA CGAAATTGCT 
GCCAAATTAA TAAGTGTAAT AGCTCATTAT ACAACAAATT GTCTTGGAAT GTGGGATGTG 
AAAGGAGTGA TTCCATCGCC AGAATTTCAA CAAATTTGTC GCCATATGCT GAAATTCCAC 
AATGGACTGG TTGGCATCAT GCCAAGAGAT CAGATCGAGG CGTTGTTCCG TCAAGTACAC 
GAAAACTTTA AAGCCAACTT ACGAGAACAC GTCACTGGAA TGGGTATCAC ACCTCATGAC 
CCTTTAAAAT ACGGATATGT CACAAAAGAT TACATGTTTT ATCAACAAAA TGTGAAAAAC 
ATGGAAAGTT GCCGTAATTT GGAGCTCGAG TCGCTTAATG ACATTATGTT TGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps-54 ( NM_001269297.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256226.1 Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), mRNA.

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CloneID	OCa344963
Clone ID Related Accession (Same CDS sequence)	NM_001269297.1 , NM_001269297.2
Accession Version	NM_001269297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Vacuolar protein sorting-associated protein 54
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269297.1.

ATGAAATCAA TGGATGACCG AACAGTAGGT CTTATATCTG CAAGTTCATC AAGAGCTTCA 
CTCAAAATTC AACCGGAGAT TGGTTATCCA AGAATATGTG ATTATTGTCA GCCAATTATC 
GAGCTTATGA CGGCTTCCGA GTTTGCACGA CATATTCGAC AAGATCATAC GACAAAAGAA 
GGTGGTAGCT TTCTTTGTAG ATACGGAGAA CATGGAGTAT GTCAGAAACT ACCACTGGAA 
GGAGTATGTG ATCTTGATTT TGAAGCCCAC ATTCGTCGTT GTCACACTTC TTCTCAACCA 
GCTGGATATT CAAGTCCAAC ATCTTCATTG GTAGCAAGTT ATACTGAAGA TAGTGAAGAA 
ACAGCTAGTT TGAGAAGTAT ACGATTGACA AGTGATCGAG ATACTCCAAC AATTGAAAAG 
AAAAAATTCA CTCTTCACAG TTTCACTCAA AATCTGTCAG CAGTTCTTGC CGATCCATCA 
CGATCTCGAA GTGACCTAAC AACGTTCTTC ACACGCCATT GGGGTGACAG TTTTGTTCCA 
ACACAACCGG TACCACAATC TAAAAGACTC GCTCGGCTCG CTGATTCGGC TTTCGATAGT 
TATTGTCAAT CTGCTGGAGA GTCCTATCGT CGATATCAGG CAATCAAAAG AGCTCTACGT 
TTGAGTCACG CCGATGGAGG AGTTGGAATT GGAGATGAAA GACAAGATGC GGAAGATCTG 
CCTACGATAT TCATCGACCC CCGATTTACA CTTGGCGACA GTTCTACTTT TTCGGATGTG 
TTCACAGTTC CTGCCAATGA TGATTTGGAT GCATTGAAAC AAACTTTATC AGGTAGAAAT 
GTGATTCCAG CTACACCTTT AGAAATTGCT ACCAACAGGA AACCTGGAGA ATTTCGAGAC 
TACGAAGCTC TTCAAAACAG ATTGGAAATG ATGCATGACG TTGTGGATGG AAGATTGGCT 
GGAAAATTAG TTGCAAAAAC TGATGATTTT TGGCAAGTCG TCCGATCGTA CAGTGGCCTC 
CAGGAACAAC TTGCCAACGC GTTGCAATGT GTGATGGTTG TTCGAAAAAA CTTGAAAAGA 
GTCGACGAAC TAGTATGCGA CCAGTCAAAA AAGATTGTAG AAGTTCATGA AAGATACGAA 
CAAAAAAAGA AACTGCTGGC AAAACTTCAT GATATTTCAT GTCTCCGAGA AGCCCAATCA 
ACAGTTCAAA TGATGTTATC ACAAGGAGAT TACCCAAAGG CTATAGAATG CATTGAAACG 
AGTCTAGATG TTCTCTCAAA GGAATTGAAC GGCGTCACTT GTTTCCGACA TCTTGCATCA 
CAGCTTCGAG AGCTTTATAC AGTAATTGGA CGTATGATGA ATGAAGATTT TGCATCATTA 
ATACAAAAAG AGCTCGGTGT GAAGCCAGAA GCTGGAACGA TGATTCAAGC CGAAGGAGAA 
CTTTCTGCTG TCCTACTTGG ATTGATGAGA ATGCGAAAGT ACTCATTTAT TGCAGTTTTA 
AGAGAGGAAA TTATTGAAGG AGTCAAAAGT GTAATGCGAC AAGTTATTAA AAATCAAATT 
CTAAATAATG GAGTTGATTT GAACGATTTT GATCCTAGTT TGACACAGTT GGGAGAGCCA 
GTACGAAGAA TGAAACATGC TGATTTTTTG AAAACTGTGA AAGCTGTTAT GGATGAAGAG 
TTCTACTTTT GTAAACGATT GGAAGCTCTA CAAGATATTC TACTGGAAAC TGTTGAACGA 
GCAAATCCTC TACATCGACA TGGCTCTGAA GATATTATAG TTGAGAGAAT CGAAGAAGCA 
AAAGATATTC ACGAATCTGA GTCTGACGAT GAAGTTGGTA CAACTTTTTC CAAATCAGCT 
AGTTCAGGAG GATTCTCCGT CGGTGGTTCG GCTTTATCCT CTAACGCGTC AGCTACCACT 
CTTCTTTCGA TTGAAGTCAG ATCAGAAGCC TTTTTACGAC ACGTGCTCCC ATTGATCGCA 
GAATTCGGTC ATCAATGTGC GCAACAACGG ATTTCACGAT TGCTCATTGC TCGGGCCAAA 
AATGCATCAG TGACAGAAGC TACTACACCT ACCCAACTAT CTGAATGCAT TAAACTCGTT 
AAAGAATTTC AATCCAAGTG TGACAAGGAA GGATGGTATT CCACTCAGAA TCAAAAAGTT 
GGAGGTCTTG GAAGATCTGT TAACAAGCTT TCTATGGATT ACATTGAAAA ATTCCATGCA 
GCTCGAAAGA TTCGTATTGG AAATATGCTT GACACAGAAC TTTGGAAAGC GACTGATGTT 
TCGATTGTGG ATCAGAACAT TGTCGACATG GCGATGGAAA CGGGCCAATT AAGAAACACA 
AAGCGAATAG ATGATGGTCC AATAAAAAAA TCATTCAAAA GAACAGAAAG CGCTGTGACA 
ATTGATTCAA CAACATCTAC TTCAAATCAA ATTCAAGGTG TAATTGTTGA TGAAGAGAAC 
TATGTTGTTG TTGGATCATC AATAACAATG ATCCAGCTCC TTTCTGACTA CTGTGAAGCC 
ATATCTGAAA TGCCATCATT TTCCCAAGAC TGGAATTCGC GAGTTGTGGA ATTACTGAAA 
ACATTTAACA GTCGGTGTTG TCAATTGATT TTAGGTGCAG GTGCCCTACA ATTGGTTGGA 
TTAAAGACTA TATCTGTTCG AAATTTAGCA CTCGCAGGTC GATCACTCGA GTTAGTATGC 
CGCTTCATTC CGATGGTTCA TGATGAGATG GACAGAGTTC TTCCTGAAAA TCGAAAAAGT 
CTGCTTCGGT ACTTCAAGCA GGTTGAAAGT GAATACCGCG ATCACGTCAA CGAAATTGCT 
GCCAAATTAA TAAGTGTAAT AGCTCATTAT ACAACAAATT GTCTTGGAAT GTGGGATGTG 
AAAGGAGTGA TTCCATCGCC AGAATTTCAA CAAATTTGTC GCCATATGCT GAAATTCCAC 
AATGGACTGG TTGGCATCAT GCCAAGAGAT CAGATCGAGG CGTTGTTCCG TCAAGTACAC 
GAAAACTTTA AAGCCAACTT ACGAGAACAC GTCACTGGAA TGGGTATCAC ACCTCATGAC 
CCTTTAAAAT ACGGATATGT CACAAAAGAT TACATGTTTT ATCAACAAAA TGTGAAAAAC 
ATGGAAAGTT GCCGTAATTT GGAGCTCGAG TCGCTTAATG ACATTATGTT TGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256226.1
CDS	14..3190
Translation	MKSMDDRTVGLISASSSRASLKIQPEIGYPRICDYCQPIIELMTASEFARHIRQDHTTKEGGSFLCRYGEHGVCQKLPLEGVCDLDFEAHIRRCHTSSQPAGYSSPTSSLVASYTEDSEETASLRSIRLTSDRDTPTIEKKKFTLHSFTQNLSAVLADPSRSRSDLTTFFTRHWGDSFVPTQPVPQSKRLARLADSAFDSYCQSAGESYRRYQAIKRALRLSHADGGVGIGDERQDAEDLPTIFIDPRFTLGDSSTFSDVFTVPANDDLDALKQTLSGRNVIPATPLEIATNRKPGEFRDYEALQNRLEMMHDVVDGRLAGKLVAKTDDFWQVVRSYSGLQEQLANALQCVMVVRKNLKRVDELVCDQSKKIVEVHERYEQKKKLLAKLHDISCLREAQSTVQMMLSQGDYPKAIECIETSLDVLSKELNGVTCFRHLASQLRELYTVIGRMMNEDFASLIQKELGVKPEAGTMIQAEGELSAVLLGLMRMRKYSFIAVLREEIIEGVKSVMRQVIKNQILNNGVDLNDFDPSLTQLGEPVRRMKHADFLKTVKAVMDEEFYFCKRLEALQDILLETVERANPLHRHGSEDIIVERIEEAKDIHESESDDEVGTTFSKSASSGGFSVGGSALSSNASATTLLSIEVRSEAFLRHVLPLIAEFGHQCAQQRISRLLIARAKNASVTEATTPTQLSECIKLVKEFQSKCDKEGWYSTQNQKVGGLGRSVNKLSMDYIEKFHAARKIRIGNMLDTELWKATDVSIVDQNIVDMAMETGQLRNTKRIDDGPIKKSFKRTESAVTIDSTTSTSNQIQGVIVDEENYVVVGSSITMIQLLSDYCEAISEMPSFSQDWNSRVVELLKTFNSRCCQLILGAGALQLVGLKTISVRNLALAGRSLELVCRFIPMVHDEMDRVLPENRKSLLRYFKQVESEYRDHVNEIAAKLISVIAHYTTNCLGMWDVKGVIPSPEFQQICRHMLKFHNGLVGIMPRDQIEALFRQVHENFKANLREHVTGMGITPHDPLKYGYVTKDYMFYQQNVKNMESCRNLELESLNDIMFE

Target ORF information:

RefSeq Version	NM_001269297.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Vacuolar protein sorting-associated protein 54 (vps-54), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269297.2

ORF Insert Sequence:

ATGAAATCAA TGGATGACCG AACAGTAGGT CTTATATCTG CAAGTTCATC AAGAGCTTCA 
CTCAAAATTC AACCGGAGAT TGGTTATCCA AGAATATGTG ATTATTGTCA GCCAATTATC 
GAGCTTATGA CGGCTTCCGA GTTTGCACGA CATATTCGAC AAGATCATAC GACAAAAGAA 
GGTGGTAGCT TTCTTTGTAG ATACGGAGAA CATGGAGTAT GTCAGAAACT ACCACTGGAA 
GGAGTATGTG ATCTTGATTT TGAAGCCCAC ATTCGTCGTT GTCACACTTC TTCTCAACCA 
GCTGGATATT CAAGTCCAAC ATCTTCATTG GTAGCAAGTT ATACTGAAGA TAGTGAAGAA 
ACAGCTAGTT TGAGAAGTAT ACGATTGACA AGTGATCGAG ATACTCCAAC AATTGAAAAG 
AAAAAATTCA CTCTTCACAG TTTCACTCAA AATCTGTCAG CAGTTCTTGC CGATCCATCA 
CGATCTCGAA GTGACCTAAC AACGTTCTTC ACACGCCATT GGGGTGACAG TTTTGTTCCA 
ACACAACCGG TACCACAATC TAAAAGACTC GCTCGGCTCG CTGATTCGGC TTTCGATAGT 
TATTGTCAAT CTGCTGGAGA GTCCTATCGT CGATATCAGG CAATCAAAAG AGCTCTACGT 
TTGAGTCACG CCGATGGAGG AGTTGGAATT GGAGATGAAA GACAAGATGC GGAAGATCTG 
CCTACGATAT TCATCGACCC CCGATTTACA CTTGGCGACA GTTCTACTTT TTCGGATGTG 
TTCACAGTTC CTGCCAATGA TGATTTGGAT GCATTGAAAC AAACTTTATC AGGTAGAAAT 
GTGATTCCAG CTACACCTTT AGAAATTGCT ACCAACAGGA AACCTGGAGA ATTTCGAGAC 
TACGAAGCTC TTCAAAACAG ATTGGAAATG ATGCATGACG TTGTGGATGG AAGATTGGCT 
GGAAAATTAG TTGCAAAAAC TGATGATTTT TGGCAAGTCG TCCGATCGTA CAGTGGCCTC 
CAGGAACAAC TTGCCAACGC GTTGCAATGT GTGATGGTTG TTCGAAAAAA CTTGAAAAGA 
GTCGACGAAC TAGTATGCGA CCAGTCAAAA AAGATTGTAG AAGTTCATGA AAGATACGAA 
CAAAAAAAGA AACTGCTGGC AAAACTTCAT GATATTTCAT GTCTCCGAGA AGCCCAATCA 
ACAGTTCAAA TGATGTTATC ACAAGGAGAT TACCCAAAGG CTATAGAATG CATTGAAACG 
AGTCTAGATG TTCTCTCAAA GGAATTGAAC GGCGTCACTT GTTTCCGACA TCTTGCATCA 
CAGCTTCGAG AGCTTTATAC AGTAATTGGA CGTATGATGA ATGAAGATTT TGCATCATTA 
ATACAAAAAG AGCTCGGTGT GAAGCCAGAA GCTGGAACGA TGATTCAAGC CGAAGGAGAA 
CTTTCTGCTG TCCTACTTGG ATTGATGAGA ATGCGAAAGT ACTCATTTAT TGCAGTTTTA 
AGAGAGGAAA TTATTGAAGG AGTCAAAAGT GTAATGCGAC AAGTTATTAA AAATCAAATT 
CTAAATAATG GAGTTGATTT GAACGATTTT GATCCTAGTT TGACACAGTT GGGAGAGCCA 
GTACGAAGAA TGAAACATGC TGATTTTTTG AAAACTGTGA AAGCTGTTAT GGATGAAGAG 
TTCTACTTTT GTAAACGATT GGAAGCTCTA CAAGATATTC TACTGGAAAC TGTTGAACGA 
GCAAATCCTC TACATCGACA TGGCTCTGAA GATATTATAG TTGAGAGAAT CGAAGAAGCA 
AAAGATATTC ACGAATCTGA GTCTGACGAT GAAGTTGGTA CAACTTTTTC CAAATCAGCT 
AGTTCAGGAG GATTCTCCGT CGGTGGTTCG GCTTTATCCT CTAACGCGTC AGCTACCACT 
CTTCTTTCGA TTGAAGTCAG ATCAGAAGCC TTTTTACGAC ACGTGCTCCC ATTGATCGCA 
GAATTCGGTC ATCAATGTGC GCAACAACGG ATTTCACGAT TGCTCATTGC TCGGGCCAAA 
AATGCATCAG TGACAGAAGC TACTACACCT ACCCAACTAT CTGAATGCAT TAAACTCGTT 
AAAGAATTTC AATCCAAGTG TGACAAGGAA GGATGGTATT CCACTCAGAA TCAAAAAGTT 
GGAGGTCTTG GAAGATCTGT TAACAAGCTT TCTATGGATT ACATTGAAAA ATTCCATGCA 
GCTCGAAAGA TTCGTATTGG AAATATGCTT GACACAGAAC TTTGGAAAGC GACTGATGTT 
TCGATTGTGG ATCAGAACAT TGTCGACATG GCGATGGAAA CGGGCCAATT AAGAAACACA 
AAGCGAATAG ATGATGGTCC AATAAAAAAA TCATTCAAAA GAACAGAAAG CGCTGTGACA 
ATTGATTCAA CAACATCTAC TTCAAATCAA ATTCAAGGTG TAATTGTTGA TGAAGAGAAC 
TATGTTGTTG TTGGATCATC AATAACAATG ATCCAGCTCC TTTCTGACTA CTGTGAAGCC 
ATATCTGAAA TGCCATCATT TTCCCAAGAC TGGAATTCGC GAGTTGTGGA ATTACTGAAA 
ACATTTAACA GTCGGTGTTG TCAATTGATT TTAGGTGCAG GTGCCCTACA ATTGGTTGGA 
TTAAAGACTA TATCTGTTCG AAATTTAGCA CTCGCAGGTC GATCACTCGA GTTAGTATGC 
CGCTTCATTC CGATGGTTCA TGATGAGATG GACAGAGTTC TTCCTGAAAA TCGAAAAAGT 
CTGCTTCGGT ACTTCAAGCA GGTTGAAAGT GAATACCGCG ATCACGTCAA CGAAATTGCT 
GCCAAATTAA TAAGTGTAAT AGCTCATTAT ACAACAAATT GTCTTGGAAT GTGGGATGTG 
AAAGGAGTGA TTCCATCGCC AGAATTTCAA CAAATTTGTC GCCATATGCT GAAATTCCAC 
AATGGACTGG TTGGCATCAT GCCAAGAGAT CAGATCGAGG CGTTGTTCCG TCAAGTACAC 
GAAAACTTTA AAGCCAACTT ACGAGAACAC GTCACTGGAA TGGGTATCAC ACCTCATGAC 
CCTTTAAAAT ACGGATATGT CACAAAAGAT TACATGTTTT ATCAACAAAA TGTGAAAAAC 
ATGGAAAGTT GCCGTAATTT GGAGCTCGAG TCGCTTAATG ACATTATGTT TGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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vps-54 cDNA ORF clone, Caenorhabditis elegans

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