VPS54 cDNA ORF clone, Homo sapiens(human)

The following VPS54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu28180 NM_016516.3
Latest version!
Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu28195 NM_001005739.2
Latest version!
Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu35432 XM_006712029.3
Latest version!
Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu89567 XM_017004316.1
Latest version!
Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OHu28180 NM_016516.2 Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu28195 NM_001005739.1 Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu28180
    Clone ID Related Accession (Same CDS sequence) NM_016516.2 , NM_016516.3
    Accession Version NM_016516.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 54 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092588.2, BC030275.1, AL514510.3, AF102177.1, BC041868.1, AK002205.1 and AW452246.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_016516.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.194861.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000272322.9/ ENSP00000272322.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT
    CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT
    AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC
    ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT
    CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT
    AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG
    CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT
    TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT
    TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT
    GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG
    TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA
    CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA
    AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG
    CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA
    ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA
    TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT
    TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGGAA
    AGACTAATAT CTCTTGTATT TGGACTTTTA AAACAAAGAA AGCTTAATTT TTTAGAAATC
    TATGGTGAAA AAATGGTTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA
    GTTTCACAAA CAGAAGAAAT AGACACAGAT GTTGTTGTGA AGCTTGCAGA TCAGATGAGA
    ATGTTGAATT TTCCCCAGTG GTTTGATCTG CTCAAGGATA TTTTCTCTAA GTTTACAATT
    TTCCTACAGA GAGTGAAGGC AACATTAAAT ATCATTCACA GTGTTGTTCT CTCAGTTCTT
    GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAGAA TGCTGCAAAA
    GATAATTCAC TGGACACAGA GGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT
    GCATTCGGTG AGGGTGAGCT AACACCTATA GCAGTTGACA CTACCTCTCA AAGAAATGCA
    TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AGCCAGAATG TACTACTGAT
    TCTTCATCCA GCAAAGAGCA CACATCATCA TCTGCTATTC CAGGAGGTGT GGATATTATG
    GTCAGTGAAG ATATGAAATT AACTGACTCA GAGCTAGGAA AGCTGGCAAA TAATATCCAG
    GAATTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CTGTCAAATT TCTCATGTCA
    AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCCATGG AATTCATAAC ACTTTCTAGA
    TTAATGGAAA CATTCATTTT AGACACCGAA CAGATCTGTG GAAGAAAAAG CACGTCATTA
    CTTGGAGCAC TTCAGAGCCA AGCTATTAAG TTTGTAAATA GGTTTCATGA AGAGAGAAAA
    ACCAAGCTCA GCCTCCTCTT AGACAATGAG CGCTGGAAGC AAGCAGATGT TCCTGCAGAA
    TTTCAGGATC TTGTTGATTC TCTGTCAGAT GGGAAGATTG CTTTACCTGA AAAAAAATCA
    GGAGCCACAG AAGAAAGGAA ACCAGCTGAA GTTCTTATTG TCGAGGGACA ACAGTATGCA
    GTTGTTGGAA CCGTATTGCT GTTAATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT
    AACATCCCAT CTGTTACTAC TGACATGCTT ACTCGTCTGT CAGATTTATT GAAGTACTTC
    AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA
    ACGATAACTA CAAAAAATTT GGCTCTTTCT TCACGATGTT TGCAGTTAAT TGTGCACTAC
    ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTACCAC CTAAGCAATA TAGCATGCTT
    AGGCATTTTG ATCATATCAC TAAGGACTAC CATGATCACA TAGCTGAAAT ATCAGCTAAG
    CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAGGCT
    CCTGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCACGAAGCT
    ATATTTGATC TCCTTCCAGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT
    TATAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT
    CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA
    GGCCTTAAAG ATTTGGACCT AAATATGGCC GAAATTTGGG AGCAGAAGAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_057600.2
    CDS512..3445
    Translation

    Target ORF information:

    RefSeq Version NM_016516.3
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_016516.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT
    CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT
    AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC
    ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT
    CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT
    AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG
    CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT
    TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT
    TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT
    GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG
    TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA
    CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA
    AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG
    CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA
    ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA
    TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT
    TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGGAA
    AGACTAATAT CTCTTGTATT TGGACTTTTA AAACAAAGAA AGCTTAATTT TTTAGAAATC
    TATGGTGAAA AAATGGTTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA
    GTTTCACAAA CAGAAGAAAT AGACACAGAT GTTGTTGTGA AGCTTGCAGA TCAGATGAGA
    ATGTTGAATT TTCCCCAGTG GTTTGATCTG CTCAAGGATA TTTTCTCTAA GTTTACAATT
    TTCCTACAGA GAGTGAAGGC AACATTAAAT ATCATTCACA GTGTTGTTCT CTCAGTTCTT
    GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAGAA TGCTGCAAAA
    GATAATTCAC TGGACACAGA GGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT
    GCATTCGGTG AGGGTGAGCT AACACCTATA GCAGTTGACA CTACCTCTCA AAGAAATGCA
    TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AGCCAGAATG TACTACTGAT
    TCTTCATCCA GCAAAGAGCA CACATCATCA TCTGCTATTC CAGGAGGTGT GGATATTATG
    GTCAGTGAAG ATATGAAATT AACTGACTCA GAGCTAGGAA AGCTGGCAAA TAATATCCAG
    GAATTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CTGTCAAATT TCTCATGTCA
    AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCCATGG AATTCATAAC ACTTTCTAGA
    TTAATGGAAA CATTCATTTT AGACACCGAA CAGATCTGTG GAAGAAAAAG CACGTCATTA
    CTTGGAGCAC TTCAGAGCCA AGCTATTAAG TTTGTAAATA GGTTTCATGA AGAGAGAAAA
    ACCAAGCTCA GCCTCCTCTT AGACAATGAG CGCTGGAAGC AAGCAGATGT TCCTGCAGAA
    TTTCAGGATC TTGTTGATTC TCTGTCAGAT GGGAAGATTG CTTTACCTGA AAAAAAATCA
    GGAGCCACAG AAGAAAGGAA ACCAGCTGAA GTTCTTATTG TCGAGGGACA ACAGTATGCA
    GTTGTTGGAA CCGTATTGCT GTTAATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT
    AACATCCCAT CTGTTACTAC TGACATGCTT ACTCGTCTGT CAGATTTATT GAAGTACTTC
    AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA
    ACGATAACTA CAAAAAATTT GGCTCTTTCT TCACGATGTT TGCAGTTAAT TGTGCACTAC
    ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTACCAC CTAAGCAATA TAGCATGCTT
    AGGCATTTTG ATCATATCAC TAAGGACTAC CATGATCACA TAGCTGAAAT ATCAGCTAAG
    CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAGGCT
    CCTGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCACGAAGCT
    ATATTTGATC TCCTTCCAGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT
    TATAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT
    CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA
    GGCCTTAAAG ATTTGGACCT AAATATGGCC GAAATTTGGG AGCAGAAGAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28195
    Clone ID Related Accession (Same CDS sequence) NM_001005739.1 , NM_001005739.2
    Accession Version NM_001005739.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-15
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 54 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC030275.1, AL514510.3, BC041868.1, AK002205.1 and AW452246.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in one of the 5' coding exons compared to transcript variant 1, resulting in a shorter isoform (2) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.54900.1, BC030275.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TATATCATTC CAAAGTAAAT
    CTCCCAGCAG CATTAAACGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA
    ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC
    AGCAAGGAAC ATTTTACAGT ATATCAACAG GAAATCTCTC AGAGAGAGAA GATTCATGAG
    AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTCGAAA GGACTCTTTT ACATACTCAT
    GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC
    TTGGATGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT
    GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTTC AAGAAAAGCT GAGCCATTAT
    CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT
    CATGCAATGA CCTCTCAACA CGAGTTGCAG GACTACCTCA GGAAAACTTC CCAGGCTGTA
    AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTCCAC
    ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA GCTGAAGTTA
    ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TATTATCTAC TTCTGAATTT
    GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAGGA ACTTCAGGGC
    ATTCACAGTT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA
    ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ACTGGAAGAT
    GACTGTCAAG TTTTAGAAGA GGAAAGACTA ATATCTCTTG TATTTGGACT TTTAAAACAA
    AGAAAGCTTA ATTTTTTAGA AATCTATGGT GAAAAAATGG TTATTACAGC AAAGAATATC
    ATTAAACAGT GTGTGATTAA TAAAGTTTCA CAAACAGAAG AAATAGACAC AGATGTTGTT
    GTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCCC AGTGGTTTGA TCTGCTCAAG
    GATATTTTCT CTAAGTTTAC AATTTTCCTA CAGAGAGTGA AGGCAACATT AAATATCATT
    CACAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT
    TCACAACAGA AGAATGCTGC AAAAGATAAT TCACTGGACA CAGAGGTGGC TTATTTAATC
    CATGAAGGCA TGTTTATAAG TGATGCATTC GGTGAGGGTG AGCTAACACC TATAGCAGTT
    GACACTACCT CTCAAAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA
    TCCGAGCCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCACACATC ATCATCTGCT
    ATTCCAGGAG GTGTGGATAT TATGGTCAGT GAAGATATGA AATTAACTGA CTCAGAGCTA
    GGAAAGCTGG CAAATAATAT CCAGGAATTA TTATATAGTG CCTCAGATAT ATGCCATGAT
    CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCC
    ATGGAATTCA TAACACTTTC TAGATTAATG GAAACATTCA TTTTAGACAC CGAACAGATC
    TGTGGAAGAA AAAGCACGTC ATTACTTGGA GCACTTCAGA GCCAAGCTAT TAAGTTTGTA
    AATAGGTTTC ATGAAGAGAG AAAAACCAAG CTCAGCCTCC TCTTAGACAA TGAGCGCTGG
    AAGCAAGCAG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTCTGTC AGATGGGAAG
    ATTGCTTTAC CTGAAAAAAA ATCAGGAGCC ACAGAAGAAA GGAAACCAGC TGAAGTTCTT
    ATTGTCGAGG GACAACAGTA TGCAGTTGTT GGAACCGTAT TGCTGTTAAT AAGAATTATC
    CTTGAATATT GCCAGTGTGT GGATAACATC CCATCTGTTA CTACTGACAT GCTTACTCGT
    CTGTCAGATT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT
    GCACTGCAAG TTGTTGGACT AAAAACGATA ACTACAAAAA ATTTGGCTCT TTCTTCACGA
    TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTA
    CCACCTAAGC AATATAGCAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAT
    CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG
    TTATCTAAGT ATGAAGTGAA GGCTCCTGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG
    CAAATGACAA AAATGCACGA AGCTATATTT GATCTCCTTC CAGAAGAACA AACACAGATG
    TTATTTTTAA GAATTAATGC AAGTTATAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA
    AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC
    ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATTTGG ACCTAAATAT GGCCGAAATT
    TGGGAGCAGA AGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001005739.1
    CDS164..3061
    Translation

    Target ORF information:

    RefSeq Version NM_001005739.1
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001005739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TATATCATTC CAAAGTAAAT
    CTCCCAGCAG CATTAAACGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA
    ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC
    AGCAAGGAAC ATTTTACAGT ATATCAACAG GAAATCTCTC AGAGAGAGAA GATTCATGAG
    AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTCGAAA GGACTCTTTT ACATACTCAT
    GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC
    TTGGATGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT
    GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTTC AAGAAAAGCT GAGCCATTAT
    CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT
    CATGCAATGA CCTCTCAACA CGAGTTGCAG GACTACCTCA GGAAAACTTC CCAGGCTGTA
    AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTCCAC
    ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA GCTGAAGTTA
    ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TATTATCTAC TTCTGAATTT
    GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAGGA ACTTCAGGGC
    ATTCACAGTT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA
    ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ACTGGAAGAT
    GACTGTCAAG TTTTAGAAGA GGAAAGACTA ATATCTCTTG TATTTGGACT TTTAAAACAA
    AGAAAGCTTA ATTTTTTAGA AATCTATGGT GAAAAAATGG TTATTACAGC AAAGAATATC
    ATTAAACAGT GTGTGATTAA TAAAGTTTCA CAAACAGAAG AAATAGACAC AGATGTTGTT
    GTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCCC AGTGGTTTGA TCTGCTCAAG
    GATATTTTCT CTAAGTTTAC AATTTTCCTA CAGAGAGTGA AGGCAACATT AAATATCATT
    CACAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT
    TCACAACAGA AGAATGCTGC AAAAGATAAT TCACTGGACA CAGAGGTGGC TTATTTAATC
    CATGAAGGCA TGTTTATAAG TGATGCATTC GGTGAGGGTG AGCTAACACC TATAGCAGTT
    GACACTACCT CTCAAAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA
    TCCGAGCCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCACACATC ATCATCTGCT
    ATTCCAGGAG GTGTGGATAT TATGGTCAGT GAAGATATGA AATTAACTGA CTCAGAGCTA
    GGAAAGCTGG CAAATAATAT CCAGGAATTA TTATATAGTG CCTCAGATAT ATGCCATGAT
    CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCC
    ATGGAATTCA TAACACTTTC TAGATTAATG GAAACATTCA TTTTAGACAC CGAACAGATC
    TGTGGAAGAA AAAGCACGTC ATTACTTGGA GCACTTCAGA GCCAAGCTAT TAAGTTTGTA
    AATAGGTTTC ATGAAGAGAG AAAAACCAAG CTCAGCCTCC TCTTAGACAA TGAGCGCTGG
    AAGCAAGCAG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTCTGTC AGATGGGAAG
    ATTGCTTTAC CTGAAAAAAA ATCAGGAGCC ACAGAAGAAA GGAAACCAGC TGAAGTTCTT
    ATTGTCGAGG GACAACAGTA TGCAGTTGTT GGAACCGTAT TGCTGTTAAT AAGAATTATC
    CTTGAATATT GCCAGTGTGT GGATAACATC CCATCTGTTA CTACTGACAT GCTTACTCGT
    CTGTCAGATT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT
    GCACTGCAAG TTGTTGGACT AAAAACGATA ACTACAAAAA ATTTGGCTCT TTCTTCACGA
    TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTA
    CCACCTAAGC AATATAGCAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAT
    CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG
    TTATCTAAGT ATGAAGTGAA GGCTCCTGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG
    CAAATGACAA AAATGCACGA AGCTATATTT GATCTCCTTC CAGAAGAACA AACACAGATG
    TTATTTTTAA GAATTAATGC AAGTTATAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA
    AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC
    ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATTTGG ACCTAAATAT GGCCGAAATT
    TGGGAGCAGA AGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28195
    Clone ID Related Accession (Same CDS sequence) NM_001005739.1 , NM_001005739.2
    Accession Version NM_001005739.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-02
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 54 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092588.2, BC030275.1, AL514510.3, BC041868.1, AK002205.1 and AW452246.1. On May 31, 2019 this sequence version replaced NM_001005739.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in one of the 5' coding exons compared to transcript variant 1, resulting in a shorter isoform (2) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.54900.1, BC030275.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TATATCATTC CAAAGTAAAT
    CTCCCAGCAG CATTAAACGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA
    ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC
    AGCAAGGAAC ATTTTACAGT ATATCAACAG GAAATCTCTC AGAGAGAGAA GATTCATGAG
    AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTCGAAA GGACTCTTTT ACATACTCAT
    GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC
    TTGGATGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT
    GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTTC AAGAAAAGCT GAGCCATTAT
    CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT
    CATGCAATGA CCTCTCAACA CGAGTTGCAG GACTACCTCA GGAAAACTTC CCAGGCTGTA
    AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTCCAC
    ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA GCTGAAGTTA
    ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TATTATCTAC TTCTGAATTT
    GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAGGA ACTTCAGGGC
    ATTCACAGTT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA
    ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ACTGGAAGAT
    GACTGTCAAG TTTTAGAAGA GGAAAGACTA ATATCTCTTG TATTTGGACT TTTAAAACAA
    AGAAAGCTTA ATTTTTTAGA AATCTATGGT GAAAAAATGG TTATTACAGC AAAGAATATC
    ATTAAACAGT GTGTGATTAA TAAAGTTTCA CAAACAGAAG AAATAGACAC AGATGTTGTT
    GTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCCC AGTGGTTTGA TCTGCTCAAG
    GATATTTTCT CTAAGTTTAC AATTTTCCTA CAGAGAGTGA AGGCAACATT AAATATCATT
    CACAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT
    TCACAACAGA AGAATGCTGC AAAAGATAAT TCACTGGACA CAGAGGTGGC TTATTTAATC
    CATGAAGGCA TGTTTATAAG TGATGCATTC GGTGAGGGTG AGCTAACACC TATAGCAGTT
    GACACTACCT CTCAAAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA
    TCCGAGCCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCACACATC ATCATCTGCT
    ATTCCAGGAG GTGTGGATAT TATGGTCAGT GAAGATATGA AATTAACTGA CTCAGAGCTA
    GGAAAGCTGG CAAATAATAT CCAGGAATTA TTATATAGTG CCTCAGATAT ATGCCATGAT
    CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCC
    ATGGAATTCA TAACACTTTC TAGATTAATG GAAACATTCA TTTTAGACAC CGAACAGATC
    TGTGGAAGAA AAAGCACGTC ATTACTTGGA GCACTTCAGA GCCAAGCTAT TAAGTTTGTA
    AATAGGTTTC ATGAAGAGAG AAAAACCAAG CTCAGCCTCC TCTTAGACAA TGAGCGCTGG
    AAGCAAGCAG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTCTGTC AGATGGGAAG
    ATTGCTTTAC CTGAAAAAAA ATCAGGAGCC ACAGAAGAAA GGAAACCAGC TGAAGTTCTT
    ATTGTCGAGG GACAACAGTA TGCAGTTGTT GGAACCGTAT TGCTGTTAAT AAGAATTATC
    CTTGAATATT GCCAGTGTGT GGATAACATC CCATCTGTTA CTACTGACAT GCTTACTCGT
    CTGTCAGATT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT
    GCACTGCAAG TTGTTGGACT AAAAACGATA ACTACAAAAA ATTTGGCTCT TTCTTCACGA
    TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTA
    CCACCTAAGC AATATAGCAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAT
    CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG
    TTATCTAAGT ATGAAGTGAA GGCTCCTGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG
    CAAATGACAA AAATGCACGA AGCTATATTT GATCTCCTTC CAGAAGAACA AACACAGATG
    TTATTTTTAA GAATTAATGC AAGTTATAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA
    AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC
    ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATTTGG ACCTAAATAT GGCCGAAATT
    TGGGAGCAGA AGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001005739.1
    CDS512..3409
    Translation

    Target ORF information:

    RefSeq Version NM_001005739.2
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001005739.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGTTAC AGATCAACAT AGATGGACTG TATATCATTC CAAAGTAAAT
    CTCCCAGCAG CATTAAACGA TCCTAGATTA GCAAAAAGAG AATCTGACTT CTTCACAAAA
    ACATGGGGAT TGGACTTTGT GGACACTGAA GTCATACCTT CATTCTACCT CCCACAGATC
    AGCAAGGAAC ATTTTACAGT ATATCAACAG GAAATCTCTC AGAGAGAGAA GATTCATGAG
    AGATGCAAGA ATATTTGTCC TCCTAAAGAT ACCTTCGAAA GGACTCTTTT ACATACTCAT
    GATAAATCCA GGACAGATCT GGAGCAAGTA CCTAAGATTT TTATGAAACC AGATTTTGCC
    TTGGATGATT CCTTAACTTT TAATTCAGTT TTACCATGGT CTCATTTTAA TACTGCTGGT
    GGAAAAGGAA ATCGTGATGC AGCTTCCTCA AAGTTGCTTC AAGAAAAGCT GAGCCATTAT
    CTGGATATTG TGGAAGTAAA CATTGCTCAC CAGATCTCTC TACGTTCAGA AGCATTTTTT
    CATGCAATGA CCTCTCAACA CGAGTTGCAG GACTACCTCA GGAAAACTTC CCAGGCTGTA
    AAAATGCTTC GAGATAAAAT TGCACAGATT GATAAAGTAA TGTGTGAAGG ATCACTCCAC
    ATTTTAAGAC TGGCACTTAC CAGAAATAAT TGTGTTAAAG TATACAATAA GCTGAAGTTA
    ATGGCCACTG TACACCAGAC TCAGCCTACA GTACAGGTGT TATTATCTAC TTCTGAATTT
    GTTGGAGCAT TGGACTTAAT AGCAACAACA CAAGAGGTTC TACAGCAGGA ACTTCAGGGC
    ATTCACAGTT TCCGGCATTT GGGATCACAG CTTTGTGAAT TAGAAAAACT GATAGATAAA
    ATGATGATTG CAGAATTTTC TACTTATTCT CACAGTGACT TAAATAGACC ACTGGAAGAT
    GACTGTCAAG TTTTAGAAGA GGAAAGACTA ATATCTCTTG TATTTGGACT TTTAAAACAA
    AGAAAGCTTA ATTTTTTAGA AATCTATGGT GAAAAAATGG TTATTACAGC AAAGAATATC
    ATTAAACAGT GTGTGATTAA TAAAGTTTCA CAAACAGAAG AAATAGACAC AGATGTTGTT
    GTGAAGCTTG CAGATCAGAT GAGAATGTTG AATTTTCCCC AGTGGTTTGA TCTGCTCAAG
    GATATTTTCT CTAAGTTTAC AATTTTCCTA CAGAGAGTGA AGGCAACATT AAATATCATT
    CACAGTGTTG TTCTCTCAGT TCTTGACAAA AACCAAAGGA CTAGAGAATT GGAAGAGATT
    TCACAACAGA AGAATGCTGC AAAAGATAAT TCACTGGACA CAGAGGTGGC TTATTTAATC
    CATGAAGGCA TGTTTATAAG TGATGCATTC GGTGAGGGTG AGCTAACACC TATAGCAGTT
    GACACTACCT CTCAAAGAAA TGCATCTCCA AATAGTGAGC CCTGCAGCAG TGATTCTGTA
    TCCGAGCCAG AATGTACTAC TGATTCTTCA TCCAGCAAAG AGCACACATC ATCATCTGCT
    ATTCCAGGAG GTGTGGATAT TATGGTCAGT GAAGATATGA AATTAACTGA CTCAGAGCTA
    GGAAAGCTGG CAAATAATAT CCAGGAATTA TTATATAGTG CCTCAGATAT ATGCCATGAT
    CGAGCTGTCA AATTTCTCAT GTCAAGAGCA AAGGATGGTT TTCTTGAGAA GCTAAATTCC
    ATGGAATTCA TAACACTTTC TAGATTAATG GAAACATTCA TTTTAGACAC CGAACAGATC
    TGTGGAAGAA AAAGCACGTC ATTACTTGGA GCACTTCAGA GCCAAGCTAT TAAGTTTGTA
    AATAGGTTTC ATGAAGAGAG AAAAACCAAG CTCAGCCTCC TCTTAGACAA TGAGCGCTGG
    AAGCAAGCAG ATGTTCCTGC AGAATTTCAG GATCTTGTTG ATTCTCTGTC AGATGGGAAG
    ATTGCTTTAC CTGAAAAAAA ATCAGGAGCC ACAGAAGAAA GGAAACCAGC TGAAGTTCTT
    ATTGTCGAGG GACAACAGTA TGCAGTTGTT GGAACCGTAT TGCTGTTAAT AAGAATTATC
    CTTGAATATT GCCAGTGTGT GGATAACATC CCATCTGTTA CTACTGACAT GCTTACTCGT
    CTGTCAGATT TATTGAAGTA CTTCAATTCA AGAAGTTGCC AGTTAGTTCT TGGAGCTGGT
    GCACTGCAAG TTGTTGGACT AAAAACGATA ACTACAAAAA ATTTGGCTCT TTCTTCACGA
    TGTTTGCAGT TAATTGTGCA CTACATTCCT GTGATCCGGG CTCATTTTGA AGCTCGACTA
    CCACCTAAGC AATATAGCAT GCTTAGGCAT TTTGATCATA TCACTAAGGA CTACCATGAT
    CACATAGCTG AAATATCAGC TAAGCTTGTA GCGATAATGG ATAGCTTATT TGACAAGCTG
    TTATCTAAGT ATGAAGTGAA GGCTCCTGTT CCTTCTGCCT GTTTCAGGAA TATTTGTAAG
    CAAATGACAA AAATGCACGA AGCTATATTT GATCTCCTTC CAGAAGAACA AACACAGATG
    TTATTTTTAA GAATTAATGC AAGTTATAAA CTCCACTTGA AAAAGCAGTT ATCTCACTTA
    AATGTGATAA ATGATGGAGG ACCTCAAAAT GGGTTGGTCA CAGCAGATGT AGCTTTTTAC
    ACTGGAAATC TTCAAGCCTT AAAAGGCCTT AAAGATTTGG ACCTAAATAT GGCCGAAATT
    TGGGAGCAGA AGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28180
    Clone ID Related Accession (Same CDS sequence) NM_016516.2 , NM_016516.3
    Accession Version NM_016516.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-30
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 54 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC030275.1, AL514510.3, AF102177.1, BC041868.1, AK002205.1 and AW452246.1. This sequence is a reference standard in the RefSeqGene project. On Oct 15, 2004 this sequence version replaced NM_016516.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.194861.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT
    CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT
    AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC
    ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT
    CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT
    AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG
    CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT
    TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT
    TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT
    GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG
    TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA
    CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA
    AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG
    CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA
    ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA
    TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT
    TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGGAA
    AGACTAATAT CTCTTGTATT TGGACTTTTA AAACAAAGAA AGCTTAATTT TTTAGAAATC
    TATGGTGAAA AAATGGTTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA
    GTTTCACAAA CAGAAGAAAT AGACACAGAT GTTGTTGTGA AGCTTGCAGA TCAGATGAGA
    ATGTTGAATT TTCCCCAGTG GTTTGATCTG CTCAAGGATA TTTTCTCTAA GTTTACAATT
    TTCCTACAGA GAGTGAAGGC AACATTAAAT ATCATTCACA GTGTTGTTCT CTCAGTTCTT
    GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAGAA TGCTGCAAAA
    GATAATTCAC TGGACACAGA GGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT
    GCATTCGGTG AGGGTGAGCT AACACCTATA GCAGTTGACA CTACCTCTCA AAGAAATGCA
    TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AGCCAGAATG TACTACTGAT
    TCTTCATCCA GCAAAGAGCA CACATCATCA TCTGCTATTC CAGGAGGTGT GGATATTATG
    GTCAGTGAAG ATATGAAATT AACTGACTCA GAGCTAGGAA AGCTGGCAAA TAATATCCAG
    GAATTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CTGTCAAATT TCTCATGTCA
    AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCCATGG AATTCATAAC ACTTTCTAGA
    TTAATGGAAA CATTCATTTT AGACACCGAA CAGATCTGTG GAAGAAAAAG CACGTCATTA
    CTTGGAGCAC TTCAGAGCCA AGCTATTAAG TTTGTAAATA GGTTTCATGA AGAGAGAAAA
    ACCAAGCTCA GCCTCCTCTT AGACAATGAG CGCTGGAAGC AAGCAGATGT TCCTGCAGAA
    TTTCAGGATC TTGTTGATTC TCTGTCAGAT GGGAAGATTG CTTTACCTGA AAAAAAATCA
    GGAGCCACAG AAGAAAGGAA ACCAGCTGAA GTTCTTATTG TCGAGGGACA ACAGTATGCA
    GTTGTTGGAA CCGTATTGCT GTTAATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT
    AACATCCCAT CTGTTACTAC TGACATGCTT ACTCGTCTGT CAGATTTATT GAAGTACTTC
    AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA
    ACGATAACTA CAAAAAATTT GGCTCTTTCT TCACGATGTT TGCAGTTAAT TGTGCACTAC
    ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTACCAC CTAAGCAATA TAGCATGCTT
    AGGCATTTTG ATCATATCAC TAAGGACTAC CATGATCACA TAGCTGAAAT ATCAGCTAAG
    CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAGGCT
    CCTGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCACGAAGCT
    ATATTTGATC TCCTTCCAGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT
    TATAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT
    CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA
    GGCCTTAAAG ATTTGGACCT AAATATGGCC GAAATTTGGG AGCAGAAGAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_057600.2
    CDS164..3097
    Translation

    Target ORF information:

    RefSeq Version NM_016516.2
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_016516.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT
    CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT
    AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC
    ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT
    CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT
    AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG
    CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT
    TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT
    TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT
    GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG
    TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA
    CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA
    AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG
    CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA
    ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA
    TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT
    TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGGAA
    AGACTAATAT CTCTTGTATT TGGACTTTTA AAACAAAGAA AGCTTAATTT TTTAGAAATC
    TATGGTGAAA AAATGGTTAT TACAGCAAAG AATATCATTA AACAGTGTGT GATTAATAAA
    GTTTCACAAA CAGAAGAAAT AGACACAGAT GTTGTTGTGA AGCTTGCAGA TCAGATGAGA
    ATGTTGAATT TTCCCCAGTG GTTTGATCTG CTCAAGGATA TTTTCTCTAA GTTTACAATT
    TTCCTACAGA GAGTGAAGGC AACATTAAAT ATCATTCACA GTGTTGTTCT CTCAGTTCTT
    GACAAAAACC AAAGGACTAG AGAATTGGAA GAGATTTCAC AACAGAAGAA TGCTGCAAAA
    GATAATTCAC TGGACACAGA GGTGGCTTAT TTAATCCATG AAGGCATGTT TATAAGTGAT
    GCATTCGGTG AGGGTGAGCT AACACCTATA GCAGTTGACA CTACCTCTCA AAGAAATGCA
    TCTCCAAATA GTGAGCCCTG CAGCAGTGAT TCTGTATCCG AGCCAGAATG TACTACTGAT
    TCTTCATCCA GCAAAGAGCA CACATCATCA TCTGCTATTC CAGGAGGTGT GGATATTATG
    GTCAGTGAAG ATATGAAATT AACTGACTCA GAGCTAGGAA AGCTGGCAAA TAATATCCAG
    GAATTATTAT ATAGTGCCTC AGATATATGC CATGATCGAG CTGTCAAATT TCTCATGTCA
    AGAGCAAAGG ATGGTTTTCT TGAGAAGCTA AATTCCATGG AATTCATAAC ACTTTCTAGA
    TTAATGGAAA CATTCATTTT AGACACCGAA CAGATCTGTG GAAGAAAAAG CACGTCATTA
    CTTGGAGCAC TTCAGAGCCA AGCTATTAAG TTTGTAAATA GGTTTCATGA AGAGAGAAAA
    ACCAAGCTCA GCCTCCTCTT AGACAATGAG CGCTGGAAGC AAGCAGATGT TCCTGCAGAA
    TTTCAGGATC TTGTTGATTC TCTGTCAGAT GGGAAGATTG CTTTACCTGA AAAAAAATCA
    GGAGCCACAG AAGAAAGGAA ACCAGCTGAA GTTCTTATTG TCGAGGGACA ACAGTATGCA
    GTTGTTGGAA CCGTATTGCT GTTAATAAGA ATTATCCTTG AATATTGCCA GTGTGTGGAT
    AACATCCCAT CTGTTACTAC TGACATGCTT ACTCGTCTGT CAGATTTATT GAAGTACTTC
    AATTCAAGAA GTTGCCAGTT AGTTCTTGGA GCTGGTGCAC TGCAAGTTGT TGGACTAAAA
    ACGATAACTA CAAAAAATTT GGCTCTTTCT TCACGATGTT TGCAGTTAAT TGTGCACTAC
    ATTCCTGTGA TCCGGGCTCA TTTTGAAGCT CGACTACCAC CTAAGCAATA TAGCATGCTT
    AGGCATTTTG ATCATATCAC TAAGGACTAC CATGATCACA TAGCTGAAAT ATCAGCTAAG
    CTTGTAGCGA TAATGGATAG CTTATTTGAC AAGCTGTTAT CTAAGTATGA AGTGAAGGCT
    CCTGTTCCTT CTGCCTGTTT CAGGAATATT TGTAAGCAAA TGACAAAAAT GCACGAAGCT
    ATATTTGATC TCCTTCCAGA AGAACAAACA CAGATGTTAT TTTTAAGAAT TAATGCAAGT
    TATAAACTCC ACTTGAAAAA GCAGTTATCT CACTTAAATG TGATAAATGA TGGAGGACCT
    CAAAATGGGT TGGTCACAGC AGATGTAGCT TTTTACACTG GAAATCTTCA AGCCTTAAAA
    GGCCTTAAAG ATTTGGACCT AAATATGGCC GAAATTTGGG AGCAGAAGAG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35432
    Clone ID Related Accession (Same CDS sequence) XM_006712029.3
    Accession Version XM_006712029.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT
    CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT
    AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC
    ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT
    CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT
    AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG
    CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT
    TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT
    TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT
    GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG
    TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA
    CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA
    AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG
    CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA
    ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA
    TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT
    TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGTGT
    GTGATTAATA AAGTTTCACA AACAGAAGAA ATAGACACAG ATGTTGTTGT GAAGCTTGCA
    GATCAGATGA GAATGTTGAA TTTTCCCCAG TGGTTTGATC TGCTCAAGGA TATTTTCTCT
    AAGTTTACAA TTTTCCTACA GAGAGTGAAG GCAACATTAA ATATCATTCA CAGTGTTGTT
    CTCTCAGTTC TTGACAAAAA CCAAAGGACT AGAGAATTGG AAGAGATTTC ACAACAGAAG
    AATGCTGCAA AAGATAATTC ACTGGACACA GAGGTGGCTT ATTTAATCCA TGAAGGCATG
    TTTATAAGTG ATGCATTCGG TGAGGGTGAG CTAACACCTA TAGCAGTTGA CACTACCTCT
    CAAAGAAATG CATCTCCAAA TAGTGAGCCC TGCAGCAGTG ATTCTGTATC CGAGCCAGAA
    TGTACTACTG ATTCTTCATC CAGCAAAGAG CACACATCAT CATCTGCTAT TCCAGGAGGT
    GTGGATATTA TGGTCAGTGA AGATATGAAA TTAACTGACT CAGAGCTAGG AAAGCTGGCA
    AATAATATCC AGGAATTATT ATATAGTGCC TCAGATATAT GCCATGATCG AGCTGTCAAA
    TTTCTCATGT CAAGAGCAAA GGATGGTTTT CTTGAGAAGC TAAATTCCAT GGAATTCATA
    ACACTTTCTA GATTAATGGA AACATTCATT TTAGACACCG AACAGATCTG TGGAAGAAAA
    AGCACGTCAT TACTTGGAGC ACTTCAGAGC CAAGCTATTA AGTTTGTAAA TAGGTTTCAT
    GAAGAGAGAA AAACCAAGCT CAGCCTCCTC TTAGACAATG AGCGCTGGAA GCAAGCAGAT
    GTTCCTGCAG AATTTCAGGA TCTTGTTGAT TCTCTGTCAG ATGGGAAGAT TGCTTTACCT
    GAAAAAAAAT CAGGAGCCAC AGAAGAAAGG AAACCAGCTG AAGTTCTTAT TGTCGAGGGA
    CAACAGTATG CAGTTGTTGG AACCGTATTG CTGTTAATAA GAATTATCCT TGAATATTGC
    CAGTGTGTGG ATAACATCCC ATCTGTTACT ACTGACATGC TTACTCGTCT GTCAGATTTA
    TTGAAGTACT TCAATTCAAG AAGTTGCCAG TTAGTTCTTG GAGCTGGTGC ACTGCAAGTT
    GTTGGACTAA AAACGATAAC TACAAAAAAT TTGGCTCTTT CTTCACGATG TTTGCAGTTA
    ATTGTGCACT ACATTCCTGT GATCCGGGCT CATTTTGAAG CTCGACTACC ACCTAAGCAA
    TATAGCATGC TTAGGCATTT TGATCATATC ACTAAGGACT ACCATGATCA CATAGCTGAA
    ATATCAGCTA AGCTTGTAGC GATAATGGAT AGCTTATTTG ACAAGCTGTT ATCTAAGTAT
    GAAGTGAAGG CTCCTGTTCC TTCTGCCTGT TTCAGGAATA TTTGTAAGCA AATGACAAAA
    ATGCACGAAG CTATATTTGA TCTCCTTCCA GAAGAACAAA CACAGATGTT ATTTTTAAGA
    ATTAATGCAA GTTATAAACT CCACTTGAAA AAGCAGTTAT CTCACTTAAA TGTGATAAAT
    GATGGAGGAC CTCAAAATGG GTTGGTCACA GCAGATGTAG CTTTTTACAC TGGAAATCTT
    CAAGCCTTAA AAGGCCTTAA AGATTTGGAC CTAAATATGG CCGAAATTTG GGAGCAGAAG
    AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712092.1
    CDS132..2957
    Translation

    Target ORF information:

    RefSeq Version XM_006712029.3
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS54, GARP complex subunit (VPS54), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712029.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCTTCAA GCCACAGTTC TTCACCAGTG CCTCAAGGAA GCAGCAGTGA TGTTTTCTTT 
    AAAATAGAGG TAGATCCGTC AAAACACATT CGACCTGTGC CATCACTGCC AGATGTGTGT
    CCCAAGGAAC CCACAGGTGA TTCACATAGT TTATATGTTG CCCCATCTCT AGTTACAGAT
    CAACATAGAT GGACTGTATA TCATTCCAAA GTAAATCTCC CAGCAGCATT AAACGATCCT
    AGATTAGCAA AAAGAGAATC TGACTTCTTC ACAAAAACAT GGGGATTGGA CTTTGTGGAC
    ACTGAAGTCA TACCTTCATT CTACCTCCCA CAGATCAGCA AGGAACATTT TACAGTATAT
    CAACAGGAAA TCTCTCAGAG AGAGAAGATT CATGAGAGAT GCAAGAATAT TTGTCCTCCT
    AAAGATACCT TCGAAAGGAC TCTTTTACAT ACTCATGATA AATCCAGGAC AGATCTGGAG
    CAAGTACCTA AGATTTTTAT GAAACCAGAT TTTGCCTTGG ATGATTCCTT AACTTTTAAT
    TCAGTTTTAC CATGGTCTCA TTTTAATACT GCTGGTGGAA AAGGAAATCG TGATGCAGCT
    TCCTCAAAGT TGCTTCAAGA AAAGCTGAGC CATTATCTGG ATATTGTGGA AGTAAACATT
    GCTCACCAGA TCTCTCTACG TTCAGAAGCA TTTTTTCATG CAATGACCTC TCAACACGAG
    TTGCAGGACT ACCTCAGGAA AACTTCCCAG GCTGTAAAAA TGCTTCGAGA TAAAATTGCA
    CAGATTGATA AAGTAATGTG TGAAGGATCA CTCCACATTT TAAGACTGGC ACTTACCAGA
    AATAATTGTG TTAAAGTATA CAATAAGCTG AAGTTAATGG CCACTGTACA CCAGACTCAG
    CCTACAGTAC AGGTGTTATT ATCTACTTCT GAATTTGTTG GAGCATTGGA CTTAATAGCA
    ACAACACAAG AGGTTCTACA GCAGGAACTT CAGGGCATTC ACAGTTTCCG GCATTTGGGA
    TCACAGCTTT GTGAATTAGA AAAACTGATA GATAAAATGA TGATTGCAGA ATTTTCTACT
    TATTCTCACA GTGACTTAAA TAGACCACTG GAAGATGACT GTCAAGTTTT AGAAGAGTGT
    GTGATTAATA AAGTTTCACA AACAGAAGAA ATAGACACAG ATGTTGTTGT GAAGCTTGCA
    GATCAGATGA GAATGTTGAA TTTTCCCCAG TGGTTTGATC TGCTCAAGGA TATTTTCTCT
    AAGTTTACAA TTTTCCTACA GAGAGTGAAG GCAACATTAA ATATCATTCA CAGTGTTGTT
    CTCTCAGTTC TTGACAAAAA CCAAAGGACT AGAGAATTGG AAGAGATTTC ACAACAGAAG
    AATGCTGCAA AAGATAATTC ACTGGACACA GAGGTGGCTT ATTTAATCCA TGAAGGCATG
    TTTATAAGTG ATGCATTCGG TGAGGGTGAG CTAACACCTA TAGCAGTTGA CACTACCTCT
    CAAAGAAATG CATCTCCAAA TAGTGAGCCC TGCAGCAGTG ATTCTGTATC CGAGCCAGAA
    TGTACTACTG ATTCTTCATC CAGCAAAGAG CACACATCAT CATCTGCTAT TCCAGGAGGT
    GTGGATATTA TGGTCAGTGA AGATATGAAA TTAACTGACT CAGAGCTAGG AAAGCTGGCA
    AATAATATCC AGGAATTATT ATATAGTGCC TCAGATATAT GCCATGATCG AGCTGTCAAA
    TTTCTCATGT CAAGAGCAAA GGATGGTTTT CTTGAGAAGC TAAATTCCAT GGAATTCATA
    ACACTTTCTA GATTAATGGA AACATTCATT TTAGACACCG AACAGATCTG TGGAAGAAAA
    AGCACGTCAT TACTTGGAGC ACTTCAGAGC CAAGCTATTA AGTTTGTAAA TAGGTTTCAT
    GAAGAGAGAA AAACCAAGCT CAGCCTCCTC TTAGACAATG AGCGCTGGAA GCAAGCAGAT
    GTTCCTGCAG AATTTCAGGA TCTTGTTGAT TCTCTGTCAG ATGGGAAGAT TGCTTTACCT
    GAAAAAAAAT CAGGAGCCAC AGAAGAAAGG AAACCAGCTG AAGTTCTTAT TGTCGAGGGA
    CAACAGTATG CAGTTGTTGG AACCGTATTG CTGTTAATAA GAATTATCCT TGAATATTGC
    CAGTGTGTGG ATAACATCCC ATCTGTTACT ACTGACATGC TTACTCGTCT GTCAGATTTA
    TTGAAGTACT TCAATTCAAG AAGTTGCCAG TTAGTTCTTG GAGCTGGTGC ACTGCAAGTT
    GTTGGACTAA AAACGATAAC TACAAAAAAT TTGGCTCTTT CTTCACGATG TTTGCAGTTA
    ATTGTGCACT ACATTCCTGT GATCCGGGCT CATTTTGAAG CTCGACTACC ACCTAAGCAA
    TATAGCATGC TTAGGCATTT TGATCATATC ACTAAGGACT ACCATGATCA CATAGCTGAA
    ATATCAGCTA AGCTTGTAGC GATAATGGAT AGCTTATTTG ACAAGCTGTT ATCTAAGTAT
    GAAGTGAAGG CTCCTGTTCC TTCTGCCTGT TTCAGGAATA TTTGTAAGCA AATGACAAAA
    ATGCACGAAG CTATATTTGA TCTCCTTCCA GAAGAACAAA CACAGATGTT ATTTTTAAGA
    ATTAATGCAA GTTATAAACT CCACTTGAAA AAGCAGTTAT CTCACTTAAA TGTGATAAAT
    GATGGAGGAC CTCAAAATGG GTTGGTCACA GCAGATGTAG CTTTTTACAC TGGAAATCTT
    CAAGCCTTAA AAGGCCTTAA AGATTTGGAC CTAAATATGG CCGAAATTTG GGAGCAGAAG
    AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89567
    Clone ID Related Accession (Same CDS sequence) XM_017004316.1
    Accession Version XM_017004316.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product vacuolar protein sorting-associated protein 54 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGTGTCC CAAGGAACCC ACAGAGAGAG AAGATTCATG AGAGATGCAA GAATATTTGT 
    CCTCCTAAAG ATACCTTCGA AAGGACTCTT TTACATACTC ATGATAAATC CAGGACAGAT
    CTGGAGCAAG TACCTAAGAT TTTTATGAAA CCAGATTTTG CCTTGGATGA TTCCTTAACT
    TTTAATTCAG TTTTACCATG GTCTCATTTT AATACTGCTG GTGGAAAAGG AAATCGTGAT
    GCAGCTTCCT CAAAGTTGCT TCAAGAAAAG CTGAGCCATT ATCTGGATAT TGTGGAAGTA
    AACATTGCTC ACCAGATCTC TCTACGTTCA GAAGCATTTT TTCATGCAAT GACCTCTCAA
    CACGAGTTGC AGGACTACCT CAGGAAAACT TCCCAGGCTG TAAAAATGCT TCGAGATAAA
    ATTGCACAGA TTGATAAAGT AATGTGTGAA GGATCACTCC ACATTTTAAG ACTGGCACTT
    ACCAGAAATA ATTGTGTTAA AGTATACAAT AAGCTGAAGT TAATGGCCAC TGTACACCAG
    ACTCAGCCTA CAGTACAGGT GTTATTATCT ACTTCTGAAT TTGTTGGAGC ATTGGACTTA
    ATAGCAACAA CACAAGAGGT TCTACAGCAG GAACTTCAGG GCATTCACAG TTTCCGGCAT
    TTGGGATCAC AGCTTTGTGA ATTAGAAAAA CTGATAGATA AAATGATGAT TGCAGAATTT
    TCTACTTATT CTCACAGTGA CTTAAATAGA CCACTGGAAG ATGACTGTCA AGTTTTAGAA
    GAGGAAAGAC TAATATCTCT TGTATTTGGA CTTTTAAAAC AAAGAAAGCT TAATTTTTTA
    GAAATCTATG GTGAAAAAAT GGTTATTACA GCAAAGAATA TCATTAAACA GTGTGTGATT
    AATAAAGTTT CACAAACAGA AGAAATAGAC ACAGATGTTG TTGTGAAGCT TGCAGATCAG
    ATGAGAATGT TGAATTTTCC CCAGTGGTTT GATCTGCTCA AGGATATTTT CTCTAAGTTT
    ACAATTTTCC TACAGAGAGT GAAGGCAACA TTAAATATCA TTCACAGTGT TGTTCTCTCA
    GTTCTTGACA AAAACCAAAG GACTAGAGAA TTGGAAGAGA TTTCACAACA GAAGAATGCT
    GCAAAAGATA ATTCACTGGA CACAGAGGTG GCTTATTTAA TCCATGAAGG CATGTTTATA
    AGTGATGCAT TCGGTGAGGG TGAGCTAACA CCTATAGCAG TTGACACTAC CTCTCAAAGA
    AATGCATCTC CAAATAGTGA GCCCTGCAGC AGTGATTCTG TATCCGAGCC AGAATGTACT
    ACTGATTCTT CATCCAGCAA AGAGCACACA TCATCATCTG CTATTCCAGG AGGTGTGGAT
    ATTATGGTCA GTGAAGATAT GAAATTAACT GACTCAGAGC TAGGAAAGCT GGCAAATAAT
    ATCCAGGAAT TATTATATAG TGCCTCAGAT ATATGCCATG ATCGAGCTGT CAAATTTCTC
    ATGTCAAGAG CAAAGGATGG TTTTCTTGAG AAGCTAAATT CCATGGAATT CATAACACTT
    TCTAGATTAA TGGAAACATT CATTTTAGAC ACCGAACAGA TCTGTGGAAG AAAAAGCACG
    TCATTACTTG GAGCACTTCA GAGCCAAGCT ATTAAGTTTG TAAATAGGTT TCATGAAGAG
    AGAAAAACCA AGCTCAGCCT CCTCTTAGAC AATGAGCGCT GGAAGCAAGC AGATGTTCCT
    GCAGAATTTC AGGATCTTGT TGATTCTCTG TCAGATGGGA AGATTGCTTT ACCTGAAAAA
    AAATCAGGAG CCACAGAAGA AAGGAAACCA GCTGAAGTTC TTATTGTCGA GGGACAACAG
    TATGCAGTTG TTGGAACCGT ATTGCTGTTA ATAAGAATTA TCCTTGAATA TTGCCAGTGT
    GTGGATAACA TCCCATCTGT TACTACTGAC ATGCTTACTC GTCTGTCAGA TTTATTGAAG
    TACTTCAATT CAAGAAGTTG CCAGTTAGTT CTTGGAGCTG GTGCACTGCA AGTTGTTGGA
    CTAAAAACGA TAACTACAAA AAATTTGGCT CTTTCTTCAC GATGTTTGCA GTTAATTGTG
    CACTACATTC CTGTGATCCG GGCTCATTTT GAAGCTCGAC TACCACCTAA GCAATATAGC
    ATGCTTAGGC ATTTTGATCA TATCACTAAG GACTACCATG ATCACATAGC TGAAATATCA
    GCTAAGCTTG TAGCGATAAT GGATAGCTTA TTTGACAAGC TGTTATCTAA GTATGAAGTG
    AAGGCTCCTG TTCCTTCTGC CTGTTTCAGG AATATTTGTA AGCAAATGAC AAAAATGCAC
    GAAGCTATAT TTGATCTCCT TCCAGAAGAA CAAACACAGA TGTTATTTTT AAGAATTAAT
    GCAAGTTATA AACTCCACTT GAAAAAGCAG TTATCTCACT TAAATGTGAT AAATGATGGA
    GGACCTCAAA ATGGGTTGGT CACAGCAGAT GTAGCTTTTT ACACTGGAAA TCTTCAAGCC
    TTAAAAGGCC TTAAAGATTT GGACCTAAAT ATGGCCGAAA TTTGGGAGCA GAAGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859805.1
    CDS198..2777
    Translation

    Target ORF information:

    RefSeq Version XM_017004316.1
    Organism Homo sapiens(human)
    Definition Homo sapiens VPS54 subunit of GARP complex (VPS54), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGTGTCC CAAGGAACCC ACAGAGAGAG AAGATTCATG AGAGATGCAA GAATATTTGT 
    CCTCCTAAAG ATACCTTCGA AAGGACTCTT TTACATACTC ATGATAAATC CAGGACAGAT
    CTGGAGCAAG TACCTAAGAT TTTTATGAAA CCAGATTTTG CCTTGGATGA TTCCTTAACT
    TTTAATTCAG TTTTACCATG GTCTCATTTT AATACTGCTG GTGGAAAAGG AAATCGTGAT
    GCAGCTTCCT CAAAGTTGCT TCAAGAAAAG CTGAGCCATT ATCTGGATAT TGTGGAAGTA
    AACATTGCTC ACCAGATCTC TCTACGTTCA GAAGCATTTT TTCATGCAAT GACCTCTCAA
    CACGAGTTGC AGGACTACCT CAGGAAAACT TCCCAGGCTG TAAAAATGCT TCGAGATAAA
    ATTGCACAGA TTGATAAAGT AATGTGTGAA GGATCACTCC ACATTTTAAG ACTGGCACTT
    ACCAGAAATA ATTGTGTTAA AGTATACAAT AAGCTGAAGT TAATGGCCAC TGTACACCAG
    ACTCAGCCTA CAGTACAGGT GTTATTATCT ACTTCTGAAT TTGTTGGAGC ATTGGACTTA
    ATAGCAACAA CACAAGAGGT TCTACAGCAG GAACTTCAGG GCATTCACAG TTTCCGGCAT
    TTGGGATCAC AGCTTTGTGA ATTAGAAAAA CTGATAGATA AAATGATGAT TGCAGAATTT
    TCTACTTATT CTCACAGTGA CTTAAATAGA CCACTGGAAG ATGACTGTCA AGTTTTAGAA
    GAGGAAAGAC TAATATCTCT TGTATTTGGA CTTTTAAAAC AAAGAAAGCT TAATTTTTTA
    GAAATCTATG GTGAAAAAAT GGTTATTACA GCAAAGAATA TCATTAAACA GTGTGTGATT
    AATAAAGTTT CACAAACAGA AGAAATAGAC ACAGATGTTG TTGTGAAGCT TGCAGATCAG
    ATGAGAATGT TGAATTTTCC CCAGTGGTTT GATCTGCTCA AGGATATTTT CTCTAAGTTT
    ACAATTTTCC TACAGAGAGT GAAGGCAACA TTAAATATCA TTCACAGTGT TGTTCTCTCA
    GTTCTTGACA AAAACCAAAG GACTAGAGAA TTGGAAGAGA TTTCACAACA GAAGAATGCT
    GCAAAAGATA ATTCACTGGA CACAGAGGTG GCTTATTTAA TCCATGAAGG CATGTTTATA
    AGTGATGCAT TCGGTGAGGG TGAGCTAACA CCTATAGCAG TTGACACTAC CTCTCAAAGA
    AATGCATCTC CAAATAGTGA GCCCTGCAGC AGTGATTCTG TATCCGAGCC AGAATGTACT
    ACTGATTCTT CATCCAGCAA AGAGCACACA TCATCATCTG CTATTCCAGG AGGTGTGGAT
    ATTATGGTCA GTGAAGATAT GAAATTAACT GACTCAGAGC TAGGAAAGCT GGCAAATAAT
    ATCCAGGAAT TATTATATAG TGCCTCAGAT ATATGCCATG ATCGAGCTGT CAAATTTCTC
    ATGTCAAGAG CAAAGGATGG TTTTCTTGAG AAGCTAAATT CCATGGAATT CATAACACTT
    TCTAGATTAA TGGAAACATT CATTTTAGAC ACCGAACAGA TCTGTGGAAG AAAAAGCACG
    TCATTACTTG GAGCACTTCA GAGCCAAGCT ATTAAGTTTG TAAATAGGTT TCATGAAGAG
    AGAAAAACCA AGCTCAGCCT CCTCTTAGAC AATGAGCGCT GGAAGCAAGC AGATGTTCCT
    GCAGAATTTC AGGATCTTGT TGATTCTCTG TCAGATGGGA AGATTGCTTT ACCTGAAAAA
    AAATCAGGAG CCACAGAAGA AAGGAAACCA GCTGAAGTTC TTATTGTCGA GGGACAACAG
    TATGCAGTTG TTGGAACCGT ATTGCTGTTA ATAAGAATTA TCCTTGAATA TTGCCAGTGT
    GTGGATAACA TCCCATCTGT TACTACTGAC ATGCTTACTC GTCTGTCAGA TTTATTGAAG
    TACTTCAATT CAAGAAGTTG CCAGTTAGTT CTTGGAGCTG GTGCACTGCA AGTTGTTGGA
    CTAAAAACGA TAACTACAAA AAATTTGGCT CTTTCTTCAC GATGTTTGCA GTTAATTGTG
    CACTACATTC CTGTGATCCG GGCTCATTTT GAAGCTCGAC TACCACCTAA GCAATATAGC
    ATGCTTAGGC ATTTTGATCA TATCACTAAG GACTACCATG ATCACATAGC TGAAATATCA
    GCTAAGCTTG TAGCGATAAT GGATAGCTTA TTTGACAAGC TGTTATCTAA GTATGAAGTG
    AAGGCTCCTG TTCCTTCTGC CTGTTTCAGG AATATTTGTA AGCAAATGAC AAAAATGCAC
    GAAGCTATAT TTGATCTCCT TCCAGAAGAA CAAACACAGA TGTTATTTTT AAGAATTAAT
    GCAAGTTATA AACTCCACTT GAAAAAGCAG TTATCTCACT TAAATGTGAT AAATGATGGA
    GGACCTCAAA ATGGGTTGGT CACAGCAGAT GTAGCTTTTT ACACTGGAAA TCTTCAAGCC
    TTAAAAGGCC TTAAAGATTT GGACCTAAAT ATGGCCGAAA TTTGGGAGCA GAAGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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