VPS35 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS35
Entrez Gene ID	106888269
Full Name	VPS35, retromer complex component
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014942374.1	XP_014797860.1	vacuolar protein sorting-associated protein 35

Pathways from BioSystems
Gene Ontology

The following VPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa65309	XM_014942374.1 Latest version!	Calidris pugnax VPS35 retromer complex component (VPS35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS35 ( XM_014942374.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014797860.1 Calidris pugnax VPS35 retromer complex component (VPS35), mRNA.

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CloneID	OCa65309
Clone ID Related Accession (Same CDS sequence)	XM_014942374.1
Accession Version	XM_014942374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	vacuolar protein sorting-associated protein 35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090842.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAT GCTTGGACAA AAACAAGCTC ATGGATGCTC TGAAACATGC TTCCAACATG 
CTTGGTGAGC TGCGGACTTC TATGTTATCT CCAAAGAGCT ATTACGAGCT CTATATGGCA 
ATTTCTGATG AACTGCACTA CTTGGAAGTC TACCTGACAG ATGAATTTGC CAAAGGAAGG 
AAAGTGGCAG ACCTTTATGA GCTAGTACAG TATGCTGGAA ATATTATTCC AAGACTGTAT 
CTCCTGATAA CAGTAGGTGT TGTCTATGTC AAGTCATTTC CACAGTCCAG GAAAGATATT 
TTGAAAGACC TGGTGGAGAT GTGCCGTGGA GTACAACATC CTCTTAGAGG CCTCTTTCTT 
AGGAACTATC TCCTGCAGTG TACCAGGAAT ATCTTGCCAG ATGAAGGCGA GCAAGCAGAT 
GAAGAAACCA CTGGAGACAT CAGTGATTCA ATGGATTTTG TGCTGCTGAA CTTTGCTGAG 
ATGAACAAAC TTTGGGTGCG AATGCAACAC CAGGGCCATA GTCGGGACAG AGAGAAAAGG 
GAGCGAGAGA GGCAGGAACT GAGAATCCTG GTAGGAACAA ACCTGGTTCG CCTCAGTCAG 
TTGGAAGGTG TTAATGTGGA GCGATACAAG CAGATTGTTC TGCCTGGAAT ATTGGAGCAA 
GTTGTAAACT GTAGAGATGC TTTAGCTCAG GAGTACCTCA TGGAGTGCAT CATACAGGTT 
TTCCCAGATG AATTTCACCT CCAAACCCTG AACCCATTTC TCAGAGCCTG TGCTGAGCTG 
CACCAAAATG TGAATGTGAA AAATATAATT ATTGCTTTAA TTGACAGGTT AGCTTTGTTT 
GCACATCGTG AAGATGGACC TGGTATCCCA GCAGATATCA AACTGTTTGA TATCTTTTCA 
CAGCAGGTTG CTACTGTTAT ACAGTCTCGC CAGGACATGC CCTCAGAGGA TGTGGTATCT 
TTGCAAGTTT CTCTCATTAA CTTGGCTATG AAGTGTTACC CGGACCGTGT TGACTATGTT 
GATAAGGTGT TGGAGACAAC TGTGGAAATA TTCAATAAAC TTAATCTGGA ACATATTGCA 
ACAAGCAGTG CTGTTTCAAA GGAGCTGACT AGACTTTTGA AAATACCCGT TGATACTTAC 
AACAATATCC TGACAGTTCT GAAATTAAAG CATTTCCACC CACTCTTTGA ATACTTTGAT 
TACGAATCCA GGAAGAGCAT GAGTTGTTAC GTGCTTAGCA ATGTATTGGA TTATAACACA 
GAAATTGTGT CCCAAGAACA GGTTGATGCT ATCATGAATT TGGTATCGAC GCTGATCCAG 
GATCAGCCGG ATCAGCCTGC TGAAGATCCT GACCCTGAGG ACTTTGCTGA TGAGCAGAGC 
CTTGTGGGAA GATTTATTCA TCTTCTGCGT TCAGATGACC CTGACCAACA GTATCTGATC 
CTGAACACTG CCCGGAAACA CTTTGGTGCA GGTGGGAACC AGCGTATTCG TTTTACACTG 
CCCCCCTTGG TTTTTGCTGC GTACCAGCTT GCTTTTCGCT ACAAAGAGAA CTCCAAAGTG 
GATGATAAAT GGGAAAAGAA GTGCCAGAAG ATCTTCTCGT TTGCTCATCA AACCATCAGT 
GCTCTGATCA AAGCCGAACT GGCGGAGCTG CCTCTCCGGC TCTTCCTACA AGGAGCACTG 
GCTGCAGGAG AGATTGGCTT TGAAAATCAT GAAACTGTGG CCTATGAATT CATGTCCCAG 
GCCTTCTCTC TATATGAAGA TGAAATCAGT GATTCAAAAG CACAGCTGGC TGCAATCACC 
TTGATAATTG GGACTTTTGA GAGGATGAAG TGCTTCAGTG AGGAGAACCA TGAGCCTTTG 
AGGACTCAGT GTGCTCTGGC AGCCTCTAAG CTCCTTAAGA AGCCAGACCA ATGCCGGGCT 
GTGAGCACTT GTGCCCATCT GTTCTGGTCT GGCCGAAATA CTGACAAGAA TGGAGAGGAG 
CTTCATGGAG GAAAAAGAGT GATGGAATGC CTAAAGAAGG CTTTGAAGAT AGCAAACCAG 
TGCATGGACC CTTCTCTGCA AGTCCAACTT TTCATAGAAA TTCTGAATAG ATATATCTAT 
TTTTATGAAA AGGAAAACGA GGCGGTGACA ATTCAGGTTT TGAATCAGCT TATACAGAAG 
ATAAGAGAAG ATCTCCCAAA CCTTGAGTCT ACTGAAGAAA CAGAACAAAT TAACAAACAC 
TTTCACAACA CACTGGAACA CTTGCGTCTG AGGAGGGAAT CACCGGAATC TGAGGGGCCA 
ATTTATGAAG GTCTTGTTCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014797860.1
CDS	85..2388
Translation	MKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEQADEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLPGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQEQVDAIMNLVSTLIQDQPDQPAEDPDPEDFADEQSLVGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQCRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENEAVTIQVLNQLIQKIREDLPNLESTEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLVL

Target ORF information:

RefSeq Version	XM_014942374.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax VPS35 retromer complex component (VPS35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014942374.1

ORF Insert Sequence:

ATGAAGAGAT GCTTGGACAA AAACAAGCTC ATGGATGCTC TGAAACATGC TTCCAACATG 
CTTGGTGAGC TGCGGACTTC TATGTTATCT CCAAAGAGCT ATTACGAGCT CTATATGGCA 
ATTTCTGATG AACTGCACTA CTTGGAAGTC TACCTGACAG ATGAATTTGC CAAAGGAAGG 
AAAGTGGCAG ACCTTTATGA GCTAGTACAG TATGCTGGAA ATATTATTCC AAGACTGTAT 
CTCCTGATAA CAGTAGGTGT TGTCTATGTC AAGTCATTTC CACAGTCCAG GAAAGATATT 
TTGAAAGACC TGGTGGAGAT GTGCCGTGGA GTACAACATC CTCTTAGAGG CCTCTTTCTT 
AGGAACTATC TCCTGCAGTG TACCAGGAAT ATCTTGCCAG ATGAAGGCGA GCAAGCAGAT 
GAAGAAACCA CTGGAGACAT CAGTGATTCA ATGGATTTTG TGCTGCTGAA CTTTGCTGAG 
ATGAACAAAC TTTGGGTGCG AATGCAACAC CAGGGCCATA GTCGGGACAG AGAGAAAAGG 
GAGCGAGAGA GGCAGGAACT GAGAATCCTG GTAGGAACAA ACCTGGTTCG CCTCAGTCAG 
TTGGAAGGTG TTAATGTGGA GCGATACAAG CAGATTGTTC TGCCTGGAAT ATTGGAGCAA 
GTTGTAAACT GTAGAGATGC TTTAGCTCAG GAGTACCTCA TGGAGTGCAT CATACAGGTT 
TTCCCAGATG AATTTCACCT CCAAACCCTG AACCCATTTC TCAGAGCCTG TGCTGAGCTG 
CACCAAAATG TGAATGTGAA AAATATAATT ATTGCTTTAA TTGACAGGTT AGCTTTGTTT 
GCACATCGTG AAGATGGACC TGGTATCCCA GCAGATATCA AACTGTTTGA TATCTTTTCA 
CAGCAGGTTG CTACTGTTAT ACAGTCTCGC CAGGACATGC CCTCAGAGGA TGTGGTATCT 
TTGCAAGTTT CTCTCATTAA CTTGGCTATG AAGTGTTACC CGGACCGTGT TGACTATGTT 
GATAAGGTGT TGGAGACAAC TGTGGAAATA TTCAATAAAC TTAATCTGGA ACATATTGCA 
ACAAGCAGTG CTGTTTCAAA GGAGCTGACT AGACTTTTGA AAATACCCGT TGATACTTAC 
AACAATATCC TGACAGTTCT GAAATTAAAG CATTTCCACC CACTCTTTGA ATACTTTGAT 
TACGAATCCA GGAAGAGCAT GAGTTGTTAC GTGCTTAGCA ATGTATTGGA TTATAACACA 
GAAATTGTGT CCCAAGAACA GGTTGATGCT ATCATGAATT TGGTATCGAC GCTGATCCAG 
GATCAGCCGG ATCAGCCTGC TGAAGATCCT GACCCTGAGG ACTTTGCTGA TGAGCAGAGC 
CTTGTGGGAA GATTTATTCA TCTTCTGCGT TCAGATGACC CTGACCAACA GTATCTGATC 
CTGAACACTG CCCGGAAACA CTTTGGTGCA GGTGGGAACC AGCGTATTCG TTTTACACTG 
CCCCCCTTGG TTTTTGCTGC GTACCAGCTT GCTTTTCGCT ACAAAGAGAA CTCCAAAGTG 
GATGATAAAT GGGAAAAGAA GTGCCAGAAG ATCTTCTCGT TTGCTCATCA AACCATCAGT 
GCTCTGATCA AAGCCGAACT GGCGGAGCTG CCTCTCCGGC TCTTCCTACA AGGAGCACTG 
GCTGCAGGAG AGATTGGCTT TGAAAATCAT GAAACTGTGG CCTATGAATT CATGTCCCAG 
GCCTTCTCTC TATATGAAGA TGAAATCAGT GATTCAAAAG CACAGCTGGC TGCAATCACC 
TTGATAATTG GGACTTTTGA GAGGATGAAG TGCTTCAGTG AGGAGAACCA TGAGCCTTTG 
AGGACTCAGT GTGCTCTGGC AGCCTCTAAG CTCCTTAAGA AGCCAGACCA ATGCCGGGCT 
GTGAGCACTT GTGCCCATCT GTTCTGGTCT GGCCGAAATA CTGACAAGAA TGGAGAGGAG 
CTTCATGGAG GAAAAAGAGT GATGGAATGC CTAAAGAAGG CTTTGAAGAT AGCAAACCAG 
TGCATGGACC CTTCTCTGCA AGTCCAACTT TTCATAGAAA TTCTGAATAG ATATATCTAT 
TTTTATGAAA AGGAAAACGA GGCGGTGACA ATTCAGGTTT TGAATCAGCT TATACAGAAG 
ATAAGAGAAG ATCTCCCAAA CCTTGAGTCT ACTGAAGAAA CAGAACAAAT TAACAAACAC 
TTTCACAACA CACTGGAACA CTTGCGTCTG AGGAGGGAAT CACCGGAATC TGAGGGGCCA 
ATTTATGAAG GTCTTGTTCT TTAG