RAPGEF2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF2
Entrez Gene ID	106889557
Full Name	Rap guanine nucleotide exchange factor 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014944711.1	XP_014800197.1	rap guanine nucleotide exchange factor 2 isoform X2
XM_014944701.1	XP_014800187.1	rap guanine nucleotide exchange factor 2 isoform X1
XM_014944719.1	XP_014800205.1	rap guanine nucleotide exchange factor 2 isoform X3
XM_014944726.1	XP_014800212.1	rap guanine nucleotide exchange factor 2 isoform X4
XM_014944735.1	XP_014800221.1	rap guanine nucleotide exchange factor 2 isoform X5

Pathways from BioSystems
Gene Ontology

The following RAPGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa58504	XM_014944701.1 Latest version!	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa58507	XM_014944726.1 Latest version!	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa58508	XM_014944735.1 Latest version!	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa58505	XM_014944711.1 Latest version!	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa58506	XM_014944719.1 Latest version!	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF2 ( XM_014944701.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014800187.1 Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa58504
Clone ID Related Accession (Same CDS sequence)	XM_014944701.1
Accession Version	XM_014944701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	rap guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTCA GTGGGTTGCC AGAGACAGCA GTGGATTCTG AGGATGATGA TGATGAAGAA 
GACATTGAAA GGGCATCAGA TCCTTTGATG AGTAGGGATA TTGTTAGAGA CTGCTTGGAG 
AAAGACCCAA TAGACAGGAC AGATGATGAC ATTGAACAAC TACTGGAATT CATGCATCAA 
TTGCCTGCTT TTGCCAACAT GACAATGTCA GTGAGGAGAG AGCTCTGTGC AGTGATGGTG 
TTTGCAGTTG TGGAGAGAGC AGGAACTATT GTACTAAACG ATGGGGAAGA GCTGGATTCC 
TGGTCAGTCA TTTTGAATGG GTCTGTGGAG GTGACATACC CTGATGGAAG AACAGAGATA 
CTCTGCATGG GGAACAGTTT TGGTGTTTCC CCTACCATGG AAAAGGAATA TATGAAAGGA 
GTGATGAGAA CCAAAGTGGA TGACTGCCAG TTTGTTTGTA TAGCCCAGCA AGATTATTGC 
CGCATCCTCA ATCAAGTGGA AAAGAACATG CAGAAGGTAG AAGAGGAAGG GGAGATTGTG 
ATGGTGAAAG AGCACAGGGA GCTTGATCGC ACTGGAACAA GAAAAGGTCA CATTGTCATC 
AAGGGAACAG CTGAAAGGTT AACAATGCAT TTGGTGGAAG AACATTCTGT GGTAGACCCA 
ACATTTATAG AAGACTTCCT GTTGACATAT CGGACCTTTC TTTCCAGCCC AATGGAAGTG 
GGCAAGAAGT TGTTGGAGTG GTTCAATGAC CCCAGCCTCA GGGATAAGGT TACACGGGTA 
GTATTGTTGT GGGTGAACAA TCACTTCAAT GATTTTGAAG GAGATCCTGC TATGACTCGA 
TTTCTGGAAG AATTTGAGAA CAATTTGGAG AGGGAGAAAA TGGGTGGACA TTTGAGGCTG 
TTAAATATTG CTTGTGCTGC TAAAGCTAAA AGAAGATTGA TAACCTTAAC AAAGCCATCT 
CGAGAAGCCC CTTTGCCTTT TATTTTGCTG GGAGGATCAG AAAAGGGATT TGGAATCTTT 
GTTGACAGTG TCGATTTTGG TAGCAAAGCT ACAGAAGCAG GCTTGAAACG TGGAGACCAG 
ATACTGGAAG TGAATGGTCA AAATTTTGAA AACATTCAGC TGTCAAAAGC CATGGAAATC 
CTTAGAAATA ACACTCATCT GTCTATCACT GTGAAAACCA ATTTATTTGT TTTTAAAGAA 
CTCTTAACGC GGTTGTCAGA GGAAAAAAGA AATGGTGCTC CCCACCTGCC TAAAATTGGT 
GATATTAAGA AGGCGAGTCG TTATTCCATC CCAGACCTTG CTGTTGATGT GGAGCAAGTT 
ATAGGATTAG AAAAAGTAAA TAAGAAAAGT AAAGCCAACA CAGTTGGGGG AAGAAATAAA 
TTAAAGAAGA TACTTGACAA GACTCGAATC AGTATTCTGC CTCAGAAACC CTACAATGAC 
ATCGGAATTG GCCAGTCTCA GGATGACAGC ATTGTGGGAC TGAGGCAAAC AAAGCACATT 
CCTCCTGCTT TACCTGTCAG TGGAACCTTG TCATCCAGTA ATCCTGATCT ACTGCAGTCT 
CATCACCGCA TCTTAGACTT CAATACTACT CCAGACTTAC CAGATCAAGT TCTGAGGGTT 
TTCAAGGCAG ACCAGCAAAG TCGCTACATC ATGATCAGTA AGGACACAAC AGCGAAAGAA 
GTGGTCATCC AGGCCATCAG GGAGTTCGCT CTCACCACGA CCCCTGACGC GTATTCACTG 
TGTGAAGTCT CTGTCACACC TGAGGGTGTC ATCAAGCAAA GGAGGCTTCC TGATCAGTTA 
TCCAAGCTTG CTGATAGGAT ACAGCTGAGT GGCAGGTACT ACCTGAAGAA CAACATGGAA 
ACAGAAACTC TTTGTTCAGA TGAAGATGCT CAAGAGTTAC TACGAGAAAG TCAAATTTCC 
CTGCTACAGC TCAGTACTGT TGAGGTGGCT ACCCAACTCT CCATGAGAAA CTTTGAACTA 
TTCCGTAATA TTGAACCCAC GGAATACATA GATGACTTGT TTAAACTCAA ATCAAAAACA 
GGTTGCACTA ATCTAAAAAA GTTTGAAGAG GTGATAAATC AAGAAACATT TTGGGTGGCT 
TCTGAGATTC TAAGAGAAAC CAACCAGTTG AAAAGGATGA AGATCATTAA GCATTTCATT 
AAGATAGCAC TGCACTGCAG AGAATGCAAG AACTTCAACT CAATGTTTGC CATCATTAGT 
GGCCTGAATT TGGCACCAGT TGCAAGGCTC CGAACTACTT GGGAAAAGCT TCCAAGCAAG 
TACGAGAAAC TGTTTCAAGA TCTACAGGAC TTGTTTGATC CATCTAGGAA TATGGCAAAA 
TACCGTAATG TCCTTAACAG CCAAAACCTG CAGCCCCCCA TTATCCCCCT GTTTCCTGTT 
ATCAAAAAAG ACCTTACGTT CCTTCATGAA GGAAATGATT CAAAAGTAGA AGGCTTGGTC 
AATTTTGAGA AGCTGAGAAT GATTGCGAAG GAGATACGCC ATGTTGGTCG CATGGCATCC 
GTGAACATGG ATCCTGCTTT AATGTTTAGA ACAAGGAAGA AGAAATGGAG GAGTTTGGGG 
TCTCTCAGCC AGGGCAGTAC GAACGCAGCG GTGCTGGATG TTGCGCAAAC AGGGGGTCAC 
AAAAAGCGGG TCCGTCGCAG CTCCTTCCTT AATGCTAAAA AACTGTATGA AGATGCTCAA 
ATGGCACGGA AGGTAAAGCA GTATCTCTCA AACTTGGACC TGGAAATGGA TGAAGAGAGC 
CTCCAGACAC TGTCTCTGCA GTGTGAGCCA GCCACCAACA CACTGCCGAA GAATACAGGA 
GACAAGCGAT CTGGAAAGTC CGAGACGTCA CCGGTGGCTC CCCGGGCAGG CATCCAGCAG 
AAAGTGCAGC AGCAGCAGCA ACATAAAGTA AACCAAGCAT TGCAGGTCCC TGCTGTATCT 
CTGTATCCCT CTCGCAAGAA GGTGCCAGTC AAAGACCTCC CACCATTCGG CATTAACTCC 
CCACAAGCTT TAAAGAAAAT TCTTTCATTA TCTGAAGAAG GAAGTTTGGA TCGTCACAAG 
AAGCAATCTG AAGACACAGT CTCAAGTGCA TCTTCACAGC TGTCTTCTCC TCCCACTTCA 
CCACAGAGCT CTCCAAGGAA AGGCTATACT TTGGCTCCTA GTGGCACGGT GGATAACTTC 
TCCGATTCTG GTCACAGTGA AATTTCTTCC AGATCAAGTA TTGTCAGCAA TTCCTCTTTT 
GACTCTATGC CGGTCTCCCT GCATGATGAG AGGAGACAGA GGCATTCTGT CAGCATTGTG 
GAGACAAATC TTGGTGTGGG AAGGATTGAT AGAAGAATCA TGATTGAACC AGATCAATAC 
AGCTTAGGCT CATATGCACC ATTGTCGGAG ACCAGAGGCC TGTATGCTGG AGCAACGGTG 
CTTTCTTCTC CTAGTACAGA AGAACTGTCA CAGGACCAGG GGGACAGAGC TTCACTTGAT 
GCAGCTGACA GTGGCCGTGG TAGCTGGACT TCTTGTTCAA GTGGTTCTCA TGACAATATA 
CAAACAATAC AGCACCAGAG AAGCTGGGAG ACTCTGCCTT TTGGACACAC TCATTTCGAT 
AGTTCAGGCG ATGGGGCAGG ACTCTGGGCC TCAGGCAGTC ATATGGACCA GCTGATGTTC 
CCCGATCATG GCACAAAGTA TGGCAGGCAA AGTCAAGGTA GGGAGGGCCT TGATCAAGCA 
CAGTCCAGAG CAAGCTGGGC ATCCTCAACA GGATACTGGG GAGAGGACTC AGAAGGTGAT 
ACAGGTACCA TAAAGCGGAG GGGTGGGAAG GATGTTTCAA TTGAAGCTGA AACCAGTAGC 
ATAACATCTG TACCAGCAGA GGAGTCAAAG CCAGCTCCCG TGCCCACTCA TATAGCAGTA 
TCACCAAGTA GTACAAAGGG GGTTATTGCA CGAAAAGAGG GTCGATATCG GGAACCACCT 
CCAACTCCTC CTGGTTATGT AGGAATACCT ATTGCTGAGT TTGCAGAAGG TGGCTCCCAT 
CCAACCAGAA AGCCTCCAGA TTACAACATA GCACTTCAGA GGTCTAGAAT GGTGGCACGG 
ACATGTGAGA CTCATGGGAC ATCAACTCCG CAGCAGCAGT CACATGGCCA TTCATCTAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCAAATGAGT CAGATCCACG TCTTGCTCAC 
TATCCCTCTC AAGGGTTTTC CACAGAGGAA GATGAAGATG AACAAGTCTC TGCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014800187.1
CDS	153..4412
Translation	MDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPVPTHIAVSPSSTKGVIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSSSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014944701.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014944701.1

ORF Insert Sequence:

ATGGATCTCA GTGGGTTGCC AGAGACAGCA GTGGATTCTG AGGATGATGA TGATGAAGAA 
GACATTGAAA GGGCATCAGA TCCTTTGATG AGTAGGGATA TTGTTAGAGA CTGCTTGGAG 
AAAGACCCAA TAGACAGGAC AGATGATGAC ATTGAACAAC TACTGGAATT CATGCATCAA 
TTGCCTGCTT TTGCCAACAT GACAATGTCA GTGAGGAGAG AGCTCTGTGC AGTGATGGTG 
TTTGCAGTTG TGGAGAGAGC AGGAACTATT GTACTAAACG ATGGGGAAGA GCTGGATTCC 
TGGTCAGTCA TTTTGAATGG GTCTGTGGAG GTGACATACC CTGATGGAAG AACAGAGATA 
CTCTGCATGG GGAACAGTTT TGGTGTTTCC CCTACCATGG AAAAGGAATA TATGAAAGGA 
GTGATGAGAA CCAAAGTGGA TGACTGCCAG TTTGTTTGTA TAGCCCAGCA AGATTATTGC 
CGCATCCTCA ATCAAGTGGA AAAGAACATG CAGAAGGTAG AAGAGGAAGG GGAGATTGTG 
ATGGTGAAAG AGCACAGGGA GCTTGATCGC ACTGGAACAA GAAAAGGTCA CATTGTCATC 
AAGGGAACAG CTGAAAGGTT AACAATGCAT TTGGTGGAAG AACATTCTGT GGTAGACCCA 
ACATTTATAG AAGACTTCCT GTTGACATAT CGGACCTTTC TTTCCAGCCC AATGGAAGTG 
GGCAAGAAGT TGTTGGAGTG GTTCAATGAC CCCAGCCTCA GGGATAAGGT TACACGGGTA 
GTATTGTTGT GGGTGAACAA TCACTTCAAT GATTTTGAAG GAGATCCTGC TATGACTCGA 
TTTCTGGAAG AATTTGAGAA CAATTTGGAG AGGGAGAAAA TGGGTGGACA TTTGAGGCTG 
TTAAATATTG CTTGTGCTGC TAAAGCTAAA AGAAGATTGA TAACCTTAAC AAAGCCATCT 
CGAGAAGCCC CTTTGCCTTT TATTTTGCTG GGAGGATCAG AAAAGGGATT TGGAATCTTT 
GTTGACAGTG TCGATTTTGG TAGCAAAGCT ACAGAAGCAG GCTTGAAACG TGGAGACCAG 
ATACTGGAAG TGAATGGTCA AAATTTTGAA AACATTCAGC TGTCAAAAGC CATGGAAATC 
CTTAGAAATA ACACTCATCT GTCTATCACT GTGAAAACCA ATTTATTTGT TTTTAAAGAA 
CTCTTAACGC GGTTGTCAGA GGAAAAAAGA AATGGTGCTC CCCACCTGCC TAAAATTGGT 
GATATTAAGA AGGCGAGTCG TTATTCCATC CCAGACCTTG CTGTTGATGT GGAGCAAGTT 
ATAGGATTAG AAAAAGTAAA TAAGAAAAGT AAAGCCAACA CAGTTGGGGG AAGAAATAAA 
TTAAAGAAGA TACTTGACAA GACTCGAATC AGTATTCTGC CTCAGAAACC CTACAATGAC 
ATCGGAATTG GCCAGTCTCA GGATGACAGC ATTGTGGGAC TGAGGCAAAC AAAGCACATT 
CCTCCTGCTT TACCTGTCAG TGGAACCTTG TCATCCAGTA ATCCTGATCT ACTGCAGTCT 
CATCACCGCA TCTTAGACTT CAATACTACT CCAGACTTAC CAGATCAAGT TCTGAGGGTT 
TTCAAGGCAG ACCAGCAAAG TCGCTACATC ATGATCAGTA AGGACACAAC AGCGAAAGAA 
GTGGTCATCC AGGCCATCAG GGAGTTCGCT CTCACCACGA CCCCTGACGC GTATTCACTG 
TGTGAAGTCT CTGTCACACC TGAGGGTGTC ATCAAGCAAA GGAGGCTTCC TGATCAGTTA 
TCCAAGCTTG CTGATAGGAT ACAGCTGAGT GGCAGGTACT ACCTGAAGAA CAACATGGAA 
ACAGAAACTC TTTGTTCAGA TGAAGATGCT CAAGAGTTAC TACGAGAAAG TCAAATTTCC 
CTGCTACAGC TCAGTACTGT TGAGGTGGCT ACCCAACTCT CCATGAGAAA CTTTGAACTA 
TTCCGTAATA TTGAACCCAC GGAATACATA GATGACTTGT TTAAACTCAA ATCAAAAACA 
GGTTGCACTA ATCTAAAAAA GTTTGAAGAG GTGATAAATC AAGAAACATT TTGGGTGGCT 
TCTGAGATTC TAAGAGAAAC CAACCAGTTG AAAAGGATGA AGATCATTAA GCATTTCATT 
AAGATAGCAC TGCACTGCAG AGAATGCAAG AACTTCAACT CAATGTTTGC CATCATTAGT 
GGCCTGAATT TGGCACCAGT TGCAAGGCTC CGAACTACTT GGGAAAAGCT TCCAAGCAAG 
TACGAGAAAC TGTTTCAAGA TCTACAGGAC TTGTTTGATC CATCTAGGAA TATGGCAAAA 
TACCGTAATG TCCTTAACAG CCAAAACCTG CAGCCCCCCA TTATCCCCCT GTTTCCTGTT 
ATCAAAAAAG ACCTTACGTT CCTTCATGAA GGAAATGATT CAAAAGTAGA AGGCTTGGTC 
AATTTTGAGA AGCTGAGAAT GATTGCGAAG GAGATACGCC ATGTTGGTCG CATGGCATCC 
GTGAACATGG ATCCTGCTTT AATGTTTAGA ACAAGGAAGA AGAAATGGAG GAGTTTGGGG 
TCTCTCAGCC AGGGCAGTAC GAACGCAGCG GTGCTGGATG TTGCGCAAAC AGGGGGTCAC 
AAAAAGCGGG TCCGTCGCAG CTCCTTCCTT AATGCTAAAA AACTGTATGA AGATGCTCAA 
ATGGCACGGA AGGTAAAGCA GTATCTCTCA AACTTGGACC TGGAAATGGA TGAAGAGAGC 
CTCCAGACAC TGTCTCTGCA GTGTGAGCCA GCCACCAACA CACTGCCGAA GAATACAGGA 
GACAAGCGAT CTGGAAAGTC CGAGACGTCA CCGGTGGCTC CCCGGGCAGG CATCCAGCAG 
AAAGTGCAGC AGCAGCAGCA ACATAAAGTA AACCAAGCAT TGCAGGTCCC TGCTGTATCT 
CTGTATCCCT CTCGCAAGAA GGTGCCAGTC AAAGACCTCC CACCATTCGG CATTAACTCC 
CCACAAGCTT TAAAGAAAAT TCTTTCATTA TCTGAAGAAG GAAGTTTGGA TCGTCACAAG 
AAGCAATCTG AAGACACAGT CTCAAGTGCA TCTTCACAGC TGTCTTCTCC TCCCACTTCA 
CCACAGAGCT CTCCAAGGAA AGGCTATACT TTGGCTCCTA GTGGCACGGT GGATAACTTC 
TCCGATTCTG GTCACAGTGA AATTTCTTCC AGATCAAGTA TTGTCAGCAA TTCCTCTTTT 
GACTCTATGC CGGTCTCCCT GCATGATGAG AGGAGACAGA GGCATTCTGT CAGCATTGTG 
GAGACAAATC TTGGTGTGGG AAGGATTGAT AGAAGAATCA TGATTGAACC AGATCAATAC 
AGCTTAGGCT CATATGCACC ATTGTCGGAG ACCAGAGGCC TGTATGCTGG AGCAACGGTG 
CTTTCTTCTC CTAGTACAGA AGAACTGTCA CAGGACCAGG GGGACAGAGC TTCACTTGAT 
GCAGCTGACA GTGGCCGTGG TAGCTGGACT TCTTGTTCAA GTGGTTCTCA TGACAATATA 
CAAACAATAC AGCACCAGAG AAGCTGGGAG ACTCTGCCTT TTGGACACAC TCATTTCGAT 
AGTTCAGGCG ATGGGGCAGG ACTCTGGGCC TCAGGCAGTC ATATGGACCA GCTGATGTTC 
CCCGATCATG GCACAAAGTA TGGCAGGCAA AGTCAAGGTA GGGAGGGCCT TGATCAAGCA 
CAGTCCAGAG CAAGCTGGGC ATCCTCAACA GGATACTGGG GAGAGGACTC AGAAGGTGAT 
ACAGGTACCA TAAAGCGGAG GGGTGGGAAG GATGTTTCAA TTGAAGCTGA AACCAGTAGC 
ATAACATCTG TACCAGCAGA GGAGTCAAAG CCAGCTCCCG TGCCCACTCA TATAGCAGTA 
TCACCAAGTA GTACAAAGGG GGTTATTGCA CGAAAAGAGG GTCGATATCG GGAACCACCT 
CCAACTCCTC CTGGTTATGT AGGAATACCT ATTGCTGAGT TTGCAGAAGG TGGCTCCCAT 
CCAACCAGAA AGCCTCCAGA TTACAACATA GCACTTCAGA GGTCTAGAAT GGTGGCACGG 
ACATGTGAGA CTCATGGGAC ATCAACTCCG CAGCAGCAGT CACATGGCCA TTCATCTAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCAAATGAGT CAGATCCACG TCTTGCTCAC 
TATCCCTCTC AAGGGTTTTC CACAGAGGAA GATGAAGATG AACAAGTCTC TGCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014944726.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014800212.1 Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa58507
Clone ID Related Accession (Same CDS sequence)	XM_014944726.1
Accession Version	XM_014944726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	rap guanine nucleotide exchange factor 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCTTC CAAACTTCCT 
GCAGATTTCA CAAAGCTGCA CCTTACTGAC AGTCTCCACC CACAGGTGAC CCACGTTTCA 
TCTAGTCACT CAGGATGTAG CATCACTAGT GACTCAGGAA GCAGCAGCCT TTCTGATATC 
TACCAGGCTA CAGAAAGTGA GGCTGGTGAT ATGGATCTCA GTGGGTTGCC AGAGACAGCA 
GTGGATTCTG AGGATGATGA TGATGAAGAA GACATTGAAA GGGCATCAGA TCCTTTGATG 
AGTAGGGATA TTGTTAGAGA CTGCTTGGAG AAAGACCCAA TAGACAGGAC AGATGATGAC 
ATTGAACAAC TACTGGAATT CATGCATCAA TTGCCTGCTT TTGCCAACAT GACAATGTCA 
GTGAGGAGAG AGCTCTGTGC AGTGATGGTG TTTGCAGTTG TGGAGAGAGC AGGAACTATT 
GTACTAAACG ATGGGGAAGA GCTGGATTCC TGGTCAGTCA TTTTGAATGG GTCTGTGGAG 
GTGACATACC CTGATGGAAG AACAGAGATA CTCTGCATGG GGAACAGTTT TGGTGTTTCC 
CCTACCATGG AAAAGGAATA TATGAAAGGA GTGATGAGAA CCAAAGTGGA TGACTGCCAG 
TTTGTTTGTA TAGCCCAGCA AGATTATTGC CGCATCCTCA ATCAAGTGGA AAAGAACATG 
CAGAAGGTAG AAGAGGAAGG GGAGATTGTG ATGGTGAAAG AGCACAGGGA GCTTGATCGC 
ACTGGAACAA GAAAAGGTCA CATTGTCATC AAGGGAACAG CTGAAAGGTT AACAATGCAT 
TTGGTGGAAG AACATTCTGT GGTAGACCCA ACATTTATAG AAGACTTCCT GTTGACATAT 
CGGACCTTTC TTTCCAGCCC AATGGAAGTG GGCAAGAAGT TGTTGGAGTG GTTCAATGAC 
CCCAGCCTCA GGGATAAGGT TACACGGGTA GTATTGTTGT GGGTGAACAA TCACTTCAAT 
GATTTTGAAG GAGATCCTGC TATGACTCGA TTTCTGGAAG AATTTGAGAA CAATTTGGAG 
AGGGAGAAAA TGGGTGGACA TTTGAGGCTG TTAAATATTG CTTGTGCTGC TAAAGCTAAA 
AGAAGATTGA TAACCTTAAC AAAGCCATCT CGAGAAGCCC CTTTGCCTTT TATTTTGCTG 
GGAGGATCAG AAAAGGGATT TGGAATCTTT GTTGACAGTG TCGATTTTGG TAGCAAAGCT 
ACAGAAGCAG GCTTGAAACG TGGAGACCAG ATACTGGAAG TGAATGGTCA AAATTTTGAA 
AACATTCAGC TGTCAAAAGC CATGGAAATC CTTAGAAATA ACACTCATCT GTCTATCACT 
GTGAAAACCA ATTTATTTGT TTTTAAAGAA CTCTTAACGC GGTTGTCAGA GGAAAAAAGA 
AATGGTGCTC CCCACCTGCC TAAAATTGGT GATATTAAGA AGGCGAGTCG TTATTCCATC 
CCAGACCTTG CTGTTGATGT GGAGCAAGTT ATAGGATTAG AAAAAGTAAA TAAGAAAAGT 
AAAGCCAACA CAGTTGGGGG AAGAAATAAA TTAAAGAAGA TACTTGACAA GACTCGAATC 
AGTATTCTGC CTCAGAAACC CTACAATGAC ATCGGAATTG GCCAGTCTCA GGATGACAGC 
ATTGTGGGAC TGAGGCAAAC AAAGCACATT CCTCCTGCTT TACCTGTCAG TGGAACCTTG 
TCATCCAGTA ATCCTGATCT ACTGCAGTCT CATCACCGCA TCTTAGACTT CAATACTACT 
CCAGACTTAC CAGATCAAGT TCTGAGGGTT TTCAAGGCAG ACCAGCAAAG TCGCTACATC 
ATGATCAGTA AGGACACAAC AGCGAAAGAA GTGGTCATCC AGGCCATCAG GGAGTTCGCT 
CTCACCACGA CCCCTGACGC GTATTCACTG TGTGAAGTCT CTGTCACACC TGAGGGTGTC 
ATCAAGCAAA GGAGGCTTCC TGATCAGTTA TCCAAGCTTG CTGATAGGAT ACAGCTGAGT 
GGCAGGTACT ACCTGAAGAA CAACATGGAA ACAGAAACTC TTTGTTCAGA TGAAGATGCT 
CAAGAGTTAC TACGAGAAAG TCAAATTTCC CTGCTACAGC TCAGTACTGT TGAGGTGGCT 
ACCCAACTCT CCATGAGAAA CTTTGAACTA TTCCGTAATA TTGAACCCAC GGAATACATA 
GATGACTTGT TTAAACTCAA ATCAAAAACA GGTTGCACTA ATCTAAAAAA GTTTGAAGAG 
GTGATAAATC AAGAAACATT TTGGGTGGCT TCTGAGATTC TAAGAGAAAC CAACCAGTTG 
AAAAGGATGA AGATCATTAA GCATTTCATT AAGATAGCAC TGCACTGCAG AGAATGCAAG 
AACTTCAACT CAATGTTTGC CATCATTAGT GGCCTGAATT TGGCACCAGT TGCAAGGCTC 
CGAACTACTT GGGAAAAGCT TCCAAGCAAG TACGAGAAAC TGTTTCAAGA TCTACAGGAC 
TTGTTTGATC CATCTAGGAA TATGGCAAAA TACCGTAATG TCCTTAACAG CCAAAACCTG 
CAGCCCCCCA TTATCCCCCT GTTTCCTGTT ATCAAAAAAG ACCTTACGTT CCTTCATGAA 
GGAAATGATT CAAAAGTAGA AGGCTTGGTC AATTTTGAGA AGCTGAGAAT GATTGCGAAG 
GAGATACGCC ATGTTGGTCG CATGGCATCC GTGAACATGG ATCCTGCTTT AATGTTTAGA 
ACAAGGAAGA AGAAATGGAG GAGTTTGGGG TCTCTCAGCC AGGGCAGTAC GAACGCAGCG 
GTGCTGGATG TTGCGCAAAC AGGGGGTCAC AAAAAGCGGG TCCGTCGCAG CTCCTTCCTT 
AATGCTAAAA AACTGTATGA AGATGCTCAA ATGGCACGGA AGGTAAAGCA GTATCTCTCA 
AACTTGGACC TGGAAATGGA TGAAGAGAGC CTCCAGACAC TGTCTCTGCA GTGTGAGCCA 
GCCACCAACA CACTGCCGAA GAATACAGGA GACAAGCGAT CTGGAAAGTC CGAGACGTCA 
CCGGTGGCTC CCCGGGCAGG CATCCAGCAG AAAGTGCAGC AGCAGCAGCA ACATAAAGTA 
AACCAAGCAT TGCAGGTCCC TGCTGTATCT CTGTATCCCT CTCGCAAGAA GGTGCCAGTC 
AAAGACCTCC CACCATTCGG CATTAACTCC CCACAAGCTT TAAAGAAAAT TCTTTCATTA 
TCTGAAGAAG GAAGTTTGGA TCGTCACAAG AAGCAATCTG AAGACACAGT CTCAAGTGCA 
TCTTCACAGC TGTCTTCTCC TCCCACTTCA CCACAGAGCT CTCCAAGGAA AGGCTATACT 
TTGGCTCCTA GTGGCACGGT GGATAACTTC TCCGATTCTG GTCACAGTGA AATTTCTTCC 
AGATCAAGTA TTGTCAGCAA TTCCTCTTTT GACTCTATGC CGGTCTCCCT GCATGATGAG 
AGGAGACAGA GGCATTCTGT CAGCATTGTG GAGACAAATC TTGGTGTGGG AAGGATTGAT 
AGAAGAATCA TGATTGAACC AGATCAATAC AGCTTAGGCT CATATGCACC ATTGTCGGAG 
ACCAGAGGCC TGTATGCTGG AGCAACGGTG CTTTCTTCTC CTAGTACAGA AGAACTGTCA 
CAGGACCAGG GGGACAGAGC TTCACTTGAT GCAGCTGACA GTGGCCGTGG TAGCTGGACT 
TCTTGTTCAA GTGGTTCTCA TGACAATATA CAAACAATAC AGCACCAGAG AAGCTGGGAG 
ACTCTGCCTT TTGGACACAC TCATTTCGAT AGTTCAGGCG ATGGGGCAGG ACTCTGGGCC 
TCAGGCAGTC ATATGGACCA GCTGATGTTC CCCGATCATG GCACAAAGTA TGGCAGGCAA 
AGTCAAGGTA GGGAGGGCCT TGATCAAGCA CAGTCCAGAG CAAGCTGGGC ATCCTCAACA 
GGATACTGGG GAGAGGACTC AGAAGGTGAT ACAGGTACCA TAAAGCGGAG GGGTGGGAAG 
GATGTTTCAA TTGAAGCTGA AACCAGTAGC ATAACATCTG TACCAGCAGA GGAGTCAAAG 
CCAGCTCCCG TGCCCACTCA TATAGCAGTA TCACCAAGTA GTACAAAGGG GGTTATTGCA 
CGAAAAGAGG GTCGATATCG GGAACCACCT CCAACTCCTC CTGGTTATGT AGGAATACCT 
ATTGCTGAGT TTGCAGAAGG TGGCTCCCAT CCAACCAGAA AGCCTCCAGA TTACAACATA 
GCACTTCAGA GGTCTAGAAT GGTGGCACGG ACATGTGAGA CTCATGGGAC ATCAACTCCG 
CAGCAGCAGT CACATGGCCA TTCATCTAGC AGGCCTGTGA ACAAACCTCA GTGGCATAAA 
CCAAATGAGT CAGATCCACG TCTTGCTCAC TATCCCTCTC AAGGGTTTTC CACAGAGGAA 
GATGAAGATG AACAAGTCTC TGCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014800212.1
CDS	16..4485
Translation	MAFLVRCYANCLQPWSSKLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPVPTHIAVSPSSTKGVIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSSSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014944726.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014944726.1

ORF Insert Sequence:

ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCTTC CAAACTTCCT 
GCAGATTTCA CAAAGCTGCA CCTTACTGAC AGTCTCCACC CACAGGTGAC CCACGTTTCA 
TCTAGTCACT CAGGATGTAG CATCACTAGT GACTCAGGAA GCAGCAGCCT TTCTGATATC 
TACCAGGCTA CAGAAAGTGA GGCTGGTGAT ATGGATCTCA GTGGGTTGCC AGAGACAGCA 
GTGGATTCTG AGGATGATGA TGATGAAGAA GACATTGAAA GGGCATCAGA TCCTTTGATG 
AGTAGGGATA TTGTTAGAGA CTGCTTGGAG AAAGACCCAA TAGACAGGAC AGATGATGAC 
ATTGAACAAC TACTGGAATT CATGCATCAA TTGCCTGCTT TTGCCAACAT GACAATGTCA 
GTGAGGAGAG AGCTCTGTGC AGTGATGGTG TTTGCAGTTG TGGAGAGAGC AGGAACTATT 
GTACTAAACG ATGGGGAAGA GCTGGATTCC TGGTCAGTCA TTTTGAATGG GTCTGTGGAG 
GTGACATACC CTGATGGAAG AACAGAGATA CTCTGCATGG GGAACAGTTT TGGTGTTTCC 
CCTACCATGG AAAAGGAATA TATGAAAGGA GTGATGAGAA CCAAAGTGGA TGACTGCCAG 
TTTGTTTGTA TAGCCCAGCA AGATTATTGC CGCATCCTCA ATCAAGTGGA AAAGAACATG 
CAGAAGGTAG AAGAGGAAGG GGAGATTGTG ATGGTGAAAG AGCACAGGGA GCTTGATCGC 
ACTGGAACAA GAAAAGGTCA CATTGTCATC AAGGGAACAG CTGAAAGGTT AACAATGCAT 
TTGGTGGAAG AACATTCTGT GGTAGACCCA ACATTTATAG AAGACTTCCT GTTGACATAT 
CGGACCTTTC TTTCCAGCCC AATGGAAGTG GGCAAGAAGT TGTTGGAGTG GTTCAATGAC 
CCCAGCCTCA GGGATAAGGT TACACGGGTA GTATTGTTGT GGGTGAACAA TCACTTCAAT 
GATTTTGAAG GAGATCCTGC TATGACTCGA TTTCTGGAAG AATTTGAGAA CAATTTGGAG 
AGGGAGAAAA TGGGTGGACA TTTGAGGCTG TTAAATATTG CTTGTGCTGC TAAAGCTAAA 
AGAAGATTGA TAACCTTAAC AAAGCCATCT CGAGAAGCCC CTTTGCCTTT TATTTTGCTG 
GGAGGATCAG AAAAGGGATT TGGAATCTTT GTTGACAGTG TCGATTTTGG TAGCAAAGCT 
ACAGAAGCAG GCTTGAAACG TGGAGACCAG ATACTGGAAG TGAATGGTCA AAATTTTGAA 
AACATTCAGC TGTCAAAAGC CATGGAAATC CTTAGAAATA ACACTCATCT GTCTATCACT 
GTGAAAACCA ATTTATTTGT TTTTAAAGAA CTCTTAACGC GGTTGTCAGA GGAAAAAAGA 
AATGGTGCTC CCCACCTGCC TAAAATTGGT GATATTAAGA AGGCGAGTCG TTATTCCATC 
CCAGACCTTG CTGTTGATGT GGAGCAAGTT ATAGGATTAG AAAAAGTAAA TAAGAAAAGT 
AAAGCCAACA CAGTTGGGGG AAGAAATAAA TTAAAGAAGA TACTTGACAA GACTCGAATC 
AGTATTCTGC CTCAGAAACC CTACAATGAC ATCGGAATTG GCCAGTCTCA GGATGACAGC 
ATTGTGGGAC TGAGGCAAAC AAAGCACATT CCTCCTGCTT TACCTGTCAG TGGAACCTTG 
TCATCCAGTA ATCCTGATCT ACTGCAGTCT CATCACCGCA TCTTAGACTT CAATACTACT 
CCAGACTTAC CAGATCAAGT TCTGAGGGTT TTCAAGGCAG ACCAGCAAAG TCGCTACATC 
ATGATCAGTA AGGACACAAC AGCGAAAGAA GTGGTCATCC AGGCCATCAG GGAGTTCGCT 
CTCACCACGA CCCCTGACGC GTATTCACTG TGTGAAGTCT CTGTCACACC TGAGGGTGTC 
ATCAAGCAAA GGAGGCTTCC TGATCAGTTA TCCAAGCTTG CTGATAGGAT ACAGCTGAGT 
GGCAGGTACT ACCTGAAGAA CAACATGGAA ACAGAAACTC TTTGTTCAGA TGAAGATGCT 
CAAGAGTTAC TACGAGAAAG TCAAATTTCC CTGCTACAGC TCAGTACTGT TGAGGTGGCT 
ACCCAACTCT CCATGAGAAA CTTTGAACTA TTCCGTAATA TTGAACCCAC GGAATACATA 
GATGACTTGT TTAAACTCAA ATCAAAAACA GGTTGCACTA ATCTAAAAAA GTTTGAAGAG 
GTGATAAATC AAGAAACATT TTGGGTGGCT TCTGAGATTC TAAGAGAAAC CAACCAGTTG 
AAAAGGATGA AGATCATTAA GCATTTCATT AAGATAGCAC TGCACTGCAG AGAATGCAAG 
AACTTCAACT CAATGTTTGC CATCATTAGT GGCCTGAATT TGGCACCAGT TGCAAGGCTC 
CGAACTACTT GGGAAAAGCT TCCAAGCAAG TACGAGAAAC TGTTTCAAGA TCTACAGGAC 
TTGTTTGATC CATCTAGGAA TATGGCAAAA TACCGTAATG TCCTTAACAG CCAAAACCTG 
CAGCCCCCCA TTATCCCCCT GTTTCCTGTT ATCAAAAAAG ACCTTACGTT CCTTCATGAA 
GGAAATGATT CAAAAGTAGA AGGCTTGGTC AATTTTGAGA AGCTGAGAAT GATTGCGAAG 
GAGATACGCC ATGTTGGTCG CATGGCATCC GTGAACATGG ATCCTGCTTT AATGTTTAGA 
ACAAGGAAGA AGAAATGGAG GAGTTTGGGG TCTCTCAGCC AGGGCAGTAC GAACGCAGCG 
GTGCTGGATG TTGCGCAAAC AGGGGGTCAC AAAAAGCGGG TCCGTCGCAG CTCCTTCCTT 
AATGCTAAAA AACTGTATGA AGATGCTCAA ATGGCACGGA AGGTAAAGCA GTATCTCTCA 
AACTTGGACC TGGAAATGGA TGAAGAGAGC CTCCAGACAC TGTCTCTGCA GTGTGAGCCA 
GCCACCAACA CACTGCCGAA GAATACAGGA GACAAGCGAT CTGGAAAGTC CGAGACGTCA 
CCGGTGGCTC CCCGGGCAGG CATCCAGCAG AAAGTGCAGC AGCAGCAGCA ACATAAAGTA 
AACCAAGCAT TGCAGGTCCC TGCTGTATCT CTGTATCCCT CTCGCAAGAA GGTGCCAGTC 
AAAGACCTCC CACCATTCGG CATTAACTCC CCACAAGCTT TAAAGAAAAT TCTTTCATTA 
TCTGAAGAAG GAAGTTTGGA TCGTCACAAG AAGCAATCTG AAGACACAGT CTCAAGTGCA 
TCTTCACAGC TGTCTTCTCC TCCCACTTCA CCACAGAGCT CTCCAAGGAA AGGCTATACT 
TTGGCTCCTA GTGGCACGGT GGATAACTTC TCCGATTCTG GTCACAGTGA AATTTCTTCC 
AGATCAAGTA TTGTCAGCAA TTCCTCTTTT GACTCTATGC CGGTCTCCCT GCATGATGAG 
AGGAGACAGA GGCATTCTGT CAGCATTGTG GAGACAAATC TTGGTGTGGG AAGGATTGAT 
AGAAGAATCA TGATTGAACC AGATCAATAC AGCTTAGGCT CATATGCACC ATTGTCGGAG 
ACCAGAGGCC TGTATGCTGG AGCAACGGTG CTTTCTTCTC CTAGTACAGA AGAACTGTCA 
CAGGACCAGG GGGACAGAGC TTCACTTGAT GCAGCTGACA GTGGCCGTGG TAGCTGGACT 
TCTTGTTCAA GTGGTTCTCA TGACAATATA CAAACAATAC AGCACCAGAG AAGCTGGGAG 
ACTCTGCCTT TTGGACACAC TCATTTCGAT AGTTCAGGCG ATGGGGCAGG ACTCTGGGCC 
TCAGGCAGTC ATATGGACCA GCTGATGTTC CCCGATCATG GCACAAAGTA TGGCAGGCAA 
AGTCAAGGTA GGGAGGGCCT TGATCAAGCA CAGTCCAGAG CAAGCTGGGC ATCCTCAACA 
GGATACTGGG GAGAGGACTC AGAAGGTGAT ACAGGTACCA TAAAGCGGAG GGGTGGGAAG 
GATGTTTCAA TTGAAGCTGA AACCAGTAGC ATAACATCTG TACCAGCAGA GGAGTCAAAG 
CCAGCTCCCG TGCCCACTCA TATAGCAGTA TCACCAAGTA GTACAAAGGG GGTTATTGCA 
CGAAAAGAGG GTCGATATCG GGAACCACCT CCAACTCCTC CTGGTTATGT AGGAATACCT 
ATTGCTGAGT TTGCAGAAGG TGGCTCCCAT CCAACCAGAA AGCCTCCAGA TTACAACATA 
GCACTTCAGA GGTCTAGAAT GGTGGCACGG ACATGTGAGA CTCATGGGAC ATCAACTCCG 
CAGCAGCAGT CACATGGCCA TTCATCTAGC AGGCCTGTGA ACAAACCTCA GTGGCATAAA 
CCAAATGAGT CAGATCCACG TCTTGCTCAC TATCCCTCTC AAGGGTTTTC CACAGAGGAA 
GATGAAGATG AACAAGTCTC TGCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014944735.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014800221.1 Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

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CloneID	OCa58508
Clone ID Related Accession (Same CDS sequence)	XM_014944735.1
Accession Version	XM_014944735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	rap guanine nucleotide exchange factor 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAGCACTCA 
CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCATCTA GTCACTCAGG ATGTAGCATC ACTAGTGACT CAGGAAGCAG CAGCCTTTCT 
GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG 
ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT 
TTGATGAGTA GGGATATTGT TAGAGACTGC TTGGAGAAAG ACCCAATAGA CAGGACAGAT 
GATGACATTG AACAACTACT GGAATTCATG CATCAATTGC CTGCTTTTGC CAACATGACA 
ATGTCAGTGA GGAGAGAGCT CTGTGCAGTG ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA 
ACTATTGTAC TAAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTTT GAATGGGTCT 
GTGGAGGTGA CATACCCTGA TGGAAGAACA GAGATACTCT GCATGGGGAA CAGTTTTGGT 
GTTTCCCCTA CCATGGAAAA GGAATATATG AAAGGAGTGA TGAGAACCAA AGTGGATGAC 
TGCCAGTTTG TTTGTATAGC CCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAG 
AACATGCAGA AGGTAGAAGA GGAAGGGGAG ATTGTGATGG TGAAAGAGCA CAGGGAGCTT 
GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCTGA AAGGTTAACA 
ATGCATTTGG TGGAAGAACA TTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG 
ACATATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGCA AGAAGTTGTT GGAGTGGTTC 
AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC 
TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT 
TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG TGCTGCTAAA 
GCTAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATT 
TTGCTGGGAG GATCAGAAAA GGGATTTGGA ATCTTTGTTG ACAGTGTCGA TTTTGGTAGC 
AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAC TGGAAGTGAA TGGTCAAAAT 
TTTGAAAACA TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT 
ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTCT TAACGCGGTT GTCAGAGGAA 
AAAAGAAATG GTGCTCCCCA CCTGCCTAAA ATTGGTGATA TTAAGAAGGC GAGTCGTTAT 
TCCATCCCAG ACCTTGCTGT TGATGTGGAG CAAGTTATAG GATTAGAAAA AGTAAATAAG 
AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAATTAA AGAAGATACT TGACAAGACT 
CGAATCAGTA TTCTGCCTCA GAAACCCTAC AATGACATCG GAATTGGCCA GTCTCAGGAT 
GACAGCATTG TGGGACTGAG GCAAACAAAG CACATTCCTC CTGCTTTACC TGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATCTACTG CAGTCTCATC ACCGCATCTT AGACTTCAAT 
ACTACTCCAG ACTTACCAGA TCAAGTTCTG AGGGTTTTCA AGGCAGACCA GCAAAGTCGC 
TACATCATGA TCAGTAAGGA CACAACAGCG AAAGAAGTGG TCATCCAGGC CATCAGGGAG 
TTCGCTCTCA CCACGACCCC TGACGCGTAT TCACTGTGTG AAGTCTCTGT CACACCTGAG 
GGTGTCATCA AGCAAAGGAG GCTTCCTGAT CAGTTATCCA AGCTTGCTGA TAGGATACAG 
CTGAGTGGCA GGTACTACCT GAAGAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAAG AGTTACTACG AGAAAGTCAA ATTTCCCTGC TACAGCTCAG TACTGTTGAG 
GTGGCTACCC AACTCTCCAT GAGAAACTTT GAACTATTCC GTAATATTGA ACCCACGGAA 
TACATAGATG ACTTGTTTAA ACTCAAATCA AAAACAGGTT GCACTAATCT AAAAAAGTTT 
GAAGAGGTGA TAAATCAAGA AACATTTTGG GTGGCTTCTG AGATTCTAAG AGAAACCAAC 
CAGTTGAAAA GGATGAAGAT CATTAAGCAT TTCATTAAGA TAGCACTGCA CTGCAGAGAA 
TGCAAGAACT TCAACTCAAT GTTTGCCATC ATTAGTGGCC TGAATTTGGC ACCAGTTGCA 
AGGCTCCGAA CTACTTGGGA AAAGCTTCCA AGCAAGTACG AGAAACTGTT TCAAGATCTA 
CAGGACTTGT TTGATCCATC TAGGAATATG GCAAAATACC GTAATGTCCT TAACAGCCAA 
AACCTGCAGC CCCCCATTAT CCCCCTGTTT CCTGTTATCA AAAAAGACCT TACGTTCCTT 
CATGAAGGAA ATGATTCAAA AGTAGAAGGC TTGGTCAATT TTGAGAAGCT GAGAATGATT 
GCGAAGGAGA TACGCCATGT TGGTCGCATG GCATCCGTGA ACATGGATCC TGCTTTAATG 
TTTAGAACAA GGAAGAAGAA ATGGAGGAGT TTGGGGTCTC TCAGCCAGGG CAGTACGAAC 
GCAGCGGTGC TGGATGTTGC GCAAACAGGG GGTCACAAAA AGCGGGTCCG TCGCAGCTCC 
TTCCTTAATG CTAAAAAACT GTATGAAGAT GCTCAAATGG CACGGAAGGT AAAGCAGTAT 
CTCTCAAACT TGGACCTGGA AATGGATGAA GAGAGCCTCC AGACACTGTC TCTGCAGTGT 
GAGCCAGCCA CCAACACACT GCCGAAGAAT ACAGGAGACA AGCGATCTGG AAAGTCCGAG 
ACGTCACCGG TGGCTCCCCG GGCAGGCATC CAGCAGAAAG TGCAGCAGCA GCAGCAACAT 
AAAGTAAACC AAGCATTGCA GGTCCCTGCT GTATCTCTGT ATCCCTCTCG CAAGAAGGTG 
CCAGTCAAAG ACCTCCCACC ATTCGGCATT AACTCCCCAC AAGCTTTAAA GAAAATTCTT 
TCATTATCTG AAGAAGGAAG TTTGGATCGT CACAAGAAGC AATCTGAAGA CACAGTCTCA 
AGTGCATCTT CACAGCTGTC TTCTCCTCCC ACTTCACCAC AGAGCTCTCC AAGGAAAGGC 
TATACTTTGG CTCCTAGTGG CACGGTGGAT AACTTCTCCG ATTCTGGTCA CAGTGAAATT 
TCTTCCAGAT CAAGTATTGT CAGCAATTCC TCTTTTGACT CTATGCCGGT CTCCCTGCAT 
GATGAGAGGA GACAGAGGCA TTCTGTCAGC ATTGTGGAGA CAAATCTTGG TGTGGGAAGG 
ATTGATAGAA GAATCATGAT TGAACCAGAT CAATACAGCT TAGGCTCATA TGCACCATTG 
TCGGAGACCA GAGGCCTGTA TGCTGGAGCA ACGGTGCTTT CTTCTCCTAG TACAGAAGAA 
CTGTCACAGG ACCAGGGGGA CAGAGCTTCA CTTGATGCAG CTGACAGTGG CCGTGGTAGC 
TGGACTTCTT GTTCAAGTGG TTCTCATGAC AATATACAAA CAATACAGCA CCAGAGAAGC 
TGGGAGACTC TGCCTTTTGG ACACACTCAT TTCGATAGTT CAGGCGATGG GGCAGGACTC 
TGGGCCTCAG GCAGTCATAT GGACCAGCTG ATGTTCCCCG ATCATGGCAC AAAGTATGGC 
AGGCAAAGTC AAGGTAGGGA GGGCCTTGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC 
TCAACAGGAT ACTGGGGAGA GGACTCAGAA GGTGATACAG GTACCATAAA GCGGAGGGGT 
GGGAAGGATG TTTCAATTGA AGCTGAAACC AGTAGCATAA CATCTGTACC AGCAGAGGAG 
TCAAAGCCAG CTCCCGTGCC CACTCATATA GCAGTATCAC CAAGTAGTAC AAAGGGGGTT 
ATTGCACGAA AAGAGGGTCG ATATCGGGAA CCACCTCCAA CTCCTCCTGG TTATGTAGGA 
ATACCTATTG CTGAGTTTGC AGAAGGTGGC TCCCATCCAA CCAGAAAGCC TCCAGATTAC 
AACATAGCAC TTCAGAGGTC TAGAATGGTG GCACGGACAT GTGAGACTCA TGGGACATCA 
ACTCCGCAGC AGCAGTCACA TGGCCATTCA TCTAGCAGGC CTGTGAACAA ACCTCAGTGG 
CATAAACCAA ATGAGTCAGA TCCACGTCTT GCTCACTATC CCTCTCAAGG GTTTTCCACA 
GAGGAAGATG AAGATGAACA AGTCTCTGCT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014800221.1
CDS	438..4913
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPVPTHIAVSPSSTKGVIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSSSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014944735.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014944735.1

ORF Insert Sequence:

ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAGCACTCA 
CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCATCTA GTCACTCAGG ATGTAGCATC ACTAGTGACT CAGGAAGCAG CAGCCTTTCT 
GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTCAGTGG GTTGCCAGAG 
ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCAGATCCT 
TTGATGAGTA GGGATATTGT TAGAGACTGC TTGGAGAAAG ACCCAATAGA CAGGACAGAT 
GATGACATTG AACAACTACT GGAATTCATG CATCAATTGC CTGCTTTTGC CAACATGACA 
ATGTCAGTGA GGAGAGAGCT CTGTGCAGTG ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA 
ACTATTGTAC TAAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTTT GAATGGGTCT 
GTGGAGGTGA CATACCCTGA TGGAAGAACA GAGATACTCT GCATGGGGAA CAGTTTTGGT 
GTTTCCCCTA CCATGGAAAA GGAATATATG AAAGGAGTGA TGAGAACCAA AGTGGATGAC 
TGCCAGTTTG TTTGTATAGC CCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTGGAAAAG 
AACATGCAGA AGGTAGAAGA GGAAGGGGAG ATTGTGATGG TGAAAGAGCA CAGGGAGCTT 
GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCTGA AAGGTTAACA 
ATGCATTTGG TGGAAGAACA TTCTGTGGTA GACCCAACAT TTATAGAAGA CTTCCTGTTG 
ACATATCGGA CCTTTCTTTC CAGCCCAATG GAAGTGGGCA AGAAGTTGTT GGAGTGGTTC 
AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC 
TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTC TGGAAGAATT TGAGAACAAT 
TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG TGCTGCTAAA 
GCTAAAAGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATT 
TTGCTGGGAG GATCAGAAAA GGGATTTGGA ATCTTTGTTG ACAGTGTCGA TTTTGGTAGC 
AAAGCTACAG AAGCAGGCTT GAAACGTGGA GACCAGATAC TGGAAGTGAA TGGTCAAAAT 
TTTGAAAACA TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT 
ATCACTGTGA AAACCAATTT ATTTGTTTTT AAAGAACTCT TAACGCGGTT GTCAGAGGAA 
AAAAGAAATG GTGCTCCCCA CCTGCCTAAA ATTGGTGATA TTAAGAAGGC GAGTCGTTAT 
TCCATCCCAG ACCTTGCTGT TGATGTGGAG CAAGTTATAG GATTAGAAAA AGTAAATAAG 
AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAATTAA AGAAGATACT TGACAAGACT 
CGAATCAGTA TTCTGCCTCA GAAACCCTAC AATGACATCG GAATTGGCCA GTCTCAGGAT 
GACAGCATTG TGGGACTGAG GCAAACAAAG CACATTCCTC CTGCTTTACC TGTCAGTGGA 
ACCTTGTCAT CCAGTAATCC TGATCTACTG CAGTCTCATC ACCGCATCTT AGACTTCAAT 
ACTACTCCAG ACTTACCAGA TCAAGTTCTG AGGGTTTTCA AGGCAGACCA GCAAAGTCGC 
TACATCATGA TCAGTAAGGA CACAACAGCG AAAGAAGTGG TCATCCAGGC CATCAGGGAG 
TTCGCTCTCA CCACGACCCC TGACGCGTAT TCACTGTGTG AAGTCTCTGT CACACCTGAG 
GGTGTCATCA AGCAAAGGAG GCTTCCTGAT CAGTTATCCA AGCTTGCTGA TAGGATACAG 
CTGAGTGGCA GGTACTACCT GAAGAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAAG AGTTACTACG AGAAAGTCAA ATTTCCCTGC TACAGCTCAG TACTGTTGAG 
GTGGCTACCC AACTCTCCAT GAGAAACTTT GAACTATTCC GTAATATTGA ACCCACGGAA 
TACATAGATG ACTTGTTTAA ACTCAAATCA AAAACAGGTT GCACTAATCT AAAAAAGTTT 
GAAGAGGTGA TAAATCAAGA AACATTTTGG GTGGCTTCTG AGATTCTAAG AGAAACCAAC 
CAGTTGAAAA GGATGAAGAT CATTAAGCAT TTCATTAAGA TAGCACTGCA CTGCAGAGAA 
TGCAAGAACT TCAACTCAAT GTTTGCCATC ATTAGTGGCC TGAATTTGGC ACCAGTTGCA 
AGGCTCCGAA CTACTTGGGA AAAGCTTCCA AGCAAGTACG AGAAACTGTT TCAAGATCTA 
CAGGACTTGT TTGATCCATC TAGGAATATG GCAAAATACC GTAATGTCCT TAACAGCCAA 
AACCTGCAGC CCCCCATTAT CCCCCTGTTT CCTGTTATCA AAAAAGACCT TACGTTCCTT 
CATGAAGGAA ATGATTCAAA AGTAGAAGGC TTGGTCAATT TTGAGAAGCT GAGAATGATT 
GCGAAGGAGA TACGCCATGT TGGTCGCATG GCATCCGTGA ACATGGATCC TGCTTTAATG 
TTTAGAACAA GGAAGAAGAA ATGGAGGAGT TTGGGGTCTC TCAGCCAGGG CAGTACGAAC 
GCAGCGGTGC TGGATGTTGC GCAAACAGGG GGTCACAAAA AGCGGGTCCG TCGCAGCTCC 
TTCCTTAATG CTAAAAAACT GTATGAAGAT GCTCAAATGG CACGGAAGGT AAAGCAGTAT 
CTCTCAAACT TGGACCTGGA AATGGATGAA GAGAGCCTCC AGACACTGTC TCTGCAGTGT 
GAGCCAGCCA CCAACACACT GCCGAAGAAT ACAGGAGACA AGCGATCTGG AAAGTCCGAG 
ACGTCACCGG TGGCTCCCCG GGCAGGCATC CAGCAGAAAG TGCAGCAGCA GCAGCAACAT 
AAAGTAAACC AAGCATTGCA GGTCCCTGCT GTATCTCTGT ATCCCTCTCG CAAGAAGGTG 
CCAGTCAAAG ACCTCCCACC ATTCGGCATT AACTCCCCAC AAGCTTTAAA GAAAATTCTT 
TCATTATCTG AAGAAGGAAG TTTGGATCGT CACAAGAAGC AATCTGAAGA CACAGTCTCA 
AGTGCATCTT CACAGCTGTC TTCTCCTCCC ACTTCACCAC AGAGCTCTCC AAGGAAAGGC 
TATACTTTGG CTCCTAGTGG CACGGTGGAT AACTTCTCCG ATTCTGGTCA CAGTGAAATT 
TCTTCCAGAT CAAGTATTGT CAGCAATTCC TCTTTTGACT CTATGCCGGT CTCCCTGCAT 
GATGAGAGGA GACAGAGGCA TTCTGTCAGC ATTGTGGAGA CAAATCTTGG TGTGGGAAGG 
ATTGATAGAA GAATCATGAT TGAACCAGAT CAATACAGCT TAGGCTCATA TGCACCATTG 
TCGGAGACCA GAGGCCTGTA TGCTGGAGCA ACGGTGCTTT CTTCTCCTAG TACAGAAGAA 
CTGTCACAGG ACCAGGGGGA CAGAGCTTCA CTTGATGCAG CTGACAGTGG CCGTGGTAGC 
TGGACTTCTT GTTCAAGTGG TTCTCATGAC AATATACAAA CAATACAGCA CCAGAGAAGC 
TGGGAGACTC TGCCTTTTGG ACACACTCAT TTCGATAGTT CAGGCGATGG GGCAGGACTC 
TGGGCCTCAG GCAGTCATAT GGACCAGCTG ATGTTCCCCG ATCATGGCAC AAAGTATGGC 
AGGCAAAGTC AAGGTAGGGA GGGCCTTGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC 
TCAACAGGAT ACTGGGGAGA GGACTCAGAA GGTGATACAG GTACCATAAA GCGGAGGGGT 
GGGAAGGATG TTTCAATTGA AGCTGAAACC AGTAGCATAA CATCTGTACC AGCAGAGGAG 
TCAAAGCCAG CTCCCGTGCC CACTCATATA GCAGTATCAC CAAGTAGTAC AAAGGGGGTT 
ATTGCACGAA AAGAGGGTCG ATATCGGGAA CCACCTCCAA CTCCTCCTGG TTATGTAGGA 
ATACCTATTG CTGAGTTTGC AGAAGGTGGC TCCCATCCAA CCAGAAAGCC TCCAGATTAC 
AACATAGCAC TTCAGAGGTC TAGAATGGTG GCACGGACAT GTGAGACTCA TGGGACATCA 
ACTCCGCAGC AGCAGTCACA TGGCCATTCA TCTAGCAGGC CTGTGAACAA ACCTCAGTGG 
CATAAACCAA ATGAGTCAGA TCCACGTCTT GCTCACTATC CCTCTCAAGG GTTTTCCACA 
GAGGAAGATG AAGATGAACA AGTCTCTGCT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014944711.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014800197.1 Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

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CloneID	OCa58505
Clone ID Related Accession (Same CDS sequence)	XM_014944711.1
Accession Version	XM_014944711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	rap guanine nucleotide exchange factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGAGG 
AGCCCCCAGG ATTTGGAGAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCTAAT 
CTGAGAGAGC ATCAGCTAAG ACTGATGTGT GAAACTGTTA GATATGAAAG GCATGAAGCA 
AATGAAGTTC TGTACTACCC CGATGATATT GGTACCTGTT GGTATATCCT GCTGTCTGGT 
TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGGAGCA GCTTTGGCAA GCGTTCTGCA 
GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCCGAAAT GATTGTGGTG 
GATTACATGG ATGAAAATGA AGAGTATTTT CAACGTCAAG CTTCTCATAG GCAGTCTCGA 
AGAAGATTTA GGAAAATCAA CCAAAAAGGG GAACGACAAA CAATTATTGA TACTGTTGAC 
CCTTATCCTG TGGGGAAGCC ACCTCTACCT AGAGGCTACC ATACGCTTCC TGCAGATTTC 
ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCTAGTCAC 
TCAGGATGTA GCATCACTAG TGACTCAGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT 
ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT 
GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGGGAT 
ATTGTTAGAG ACTGCTTGGA GAAAGACCCA ATAGACAGGA CAGATGATGA CATTGAACAA 
CTACTGGAAT TCATGCATCA ATTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA 
GAGCTCTGTG CAGTGATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTAAAC 
GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTTTGAATG GGTCTGTGGA GGTGACATAC 
CCTGATGGAA GAACAGAGAT ACTCTGCATG GGGAACAGTT TTGGTGTTTC CCCTACCATG 
GAAAAGGAAT ATATGAAAGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT 
ATAGCCCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAGAACAT GCAGAAGGTA 
GAAGAGGAAG GGGAGATTGT GATGGTGAAA GAGCACAGGG AGCTTGATCG CACTGGAACA 
AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCTGAAAGGT TAACAATGCA TTTGGTGGAA 
GAACATTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACATA TCGGACCTTT 
CTTTCCAGCC CAATGGAAGT GGGCAAGAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC 
AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA 
GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA 
ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGTGCTG CTAAAGCTAA AAGAAGATTG 
ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATTTTGCT GGGAGGATCA 
GAAAAGGGAT TTGGAATCTT TGTTGACAGT GTCGATTTTG GTAGCAAAGC TACAGAAGCA 
GGCTTGAAAC GTGGAGACCA GATACTGGAA GTGAATGGTC AAAATTTTGA AAACATTCAG 
CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC 
AATTTATTTG TTTTTAAAGA ACTCTTAACG CGGTTGTCAG AGGAAAAAAG AAATGGTGCT 
CCCCACCTGC CTAAAATTGG TGATATTAAG AAGGCGAGTC GTTATTCCAT CCCAGACCTT 
GCTGTTGATG TGGAGCAAGT TATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC 
ACAGTTGGGG GAAGAAATAA ATTAAAGAAG ATACTTGACA AGACTCGAAT CAGTATTCTG 
CCTCAGAAAC CCTACAATGA CATCGGAATT GGCCAGTCTC AGGATGACAG CATTGTGGGA 
CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCTT GTCATCCAGT 
AATCCTGATC TACTGCAGTC TCATCACCGC ATCTTAGACT TCAATACTAC TCCAGACTTA 
CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GACCAGCAAA GTCGCTACAT CATGATCAGT 
AAGGACACAA CAGCGAAAGA AGTGGTCATC CAGGCCATCA GGGAGTTCGC TCTCACCACG 
ACCCCTGACG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT CATCAAGCAA 
AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGATAGGA TACAGCTGAG TGGCAGGTAC 
TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAAGAGTTA 
CTACGAGAAA GTCAAATTTC CCTGCTACAG CTCAGTACTG TTGAGGTGGC TACCCAACTC 
TCCATGAGAA ACTTTGAACT ATTCCGTAAT ATTGAACCCA CGGAATACAT AGATGACTTG 
TTTAAACTCA AATCAAAAAC AGGTTGCACT AATCTAAAAA AGTTTGAAGA GGTGATAAAT 
CAAGAAACAT TTTGGGTGGC TTCTGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG 
AAGATCATTA AGCATTTCAT TAAGATAGCA CTGCACTGCA GAGAATGCAA GAACTTCAAC 
TCAATGTTTG CCATCATTAG TGGCCTGAAT TTGGCACCAG TTGCAAGGCT CCGAACTACT 
TGGGAAAAGC TTCCAAGCAA GTACGAGAAA CTGTTTCAAG ATCTACAGGA CTTGTTTGAT 
CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAACCT GCAGCCCCCC 
ATTATCCCCC TGTTTCCTGT TATCAAAAAA GACCTTACGT TCCTTCATGA AGGAAATGAT 
TCAAAAGTAG AAGGCTTGGT CAATTTTGAG AAGCTGAGAA TGATTGCGAA GGAGATACGC 
CATGTTGGTC GCATGGCATC CGTGAACATG GATCCTGCTT TAATGTTTAG AACAAGGAAG 
AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CGAACGCAGC GGTGCTGGAT 
GTTGCGCAAA CAGGGGGTCA CAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAATGCTAAA 
AAACTGTATG AAGATGCTCA AATGGCACGG AAGGTAAAGC AGTATCTCTC AAACTTGGAC 
CTGGAAATGG ATGAAGAGAG CCTCCAGACA CTGTCTCTGC AGTGTGAGCC AGCCACCAAC 
ACACTGCCGA AGAATACAGG AGACAAGCGA TCTGGAAAGT CCGAGACGTC ACCGGTGGCT 
CCCCGGGCAG GCATCCAGCA GAAAGTGCAG CAGCAGCAGC AACATAAAGT AAACCAAGCA 
TTGCAGGTCC CTGCTGTATC TCTGTATCCC TCTCGCAAGA AGGTGCCAGT CAAAGACCTC 
CCACCATTCG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA 
GGAAGTTTGG ATCGTCACAA GAAGCAATCT GAAGACACAG TCTCAAGTGC ATCTTCACAG 
CTGTCTTCTC CTCCCACTTC ACCACAGAGC TCTCCAAGGA AAGGCTATAC TTTGGCTCCT 
AGTGGCACGG TGGATAACTT CTCCGATTCT GGTCACAGTG AAATTTCTTC CAGATCAAGT 
ATTGTCAGCA ATTCCTCTTT TGACTCTATG CCGGTCTCCC TGCATGATGA GAGGAGACAG 
AGGCATTCTG TCAGCATTGT GGAGACAAAT CTTGGTGTGG GAAGGATTGA TAGAAGAATC 
ATGATTGAAC CAGATCAATA CAGCTTAGGC TCATATGCAC CATTGTCGGA GACCAGAGGC 
CTGTATGCTG GAGCAACGGT GCTTTCTTCT CCTAGTACAG AAGAACTGTC ACAGGACCAG 
GGGGACAGAG CTTCACTTGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA 
AGTGGTTCTC ATGACAATAT ACAAACAATA CAGCACCAGA GAAGCTGGGA GACTCTGCCT 
TTTGGACACA CTCATTTCGA TAGTTCAGGC GATGGGGCAG GACTCTGGGC CTCAGGCAGT 
CATATGGACC AGCTGATGTT CCCCGATCAT GGCACAAAGT ATGGCAGGCA AAGTCAAGGT 
AGGGAGGGCC TTGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC AGGATACTGG 
GGAGAGGACT CAGAAGGTGA TACAGGTACC ATAAAGCGGA GGGGTGGGAA GGATGTTTCA 
ATTGAAGCTG AAACCAGTAG CATAACATCT GTACCAGCAG AGGAGTCAAA GCCAGCTCCC 
GTGCCCACTC ATATAGCAGT ATCACCAAGT AGTACAAAGG GGGTTATTGC ACGAAAAGAG 
GGTCGATATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG 
TTTGCAGAAG GTGGCTCCCA TCCAACCAGA AAGCCTCCAG ATTACAACAT AGCACTTCAG 
AGGTCTAGAA TGGTGGCACG GACATGTGAG ACTCATGGGA CATCAACTCC GCAGCAGCAG 
TCACATGGCC ATTCATCTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAATGAG 
TCAGATCCAC GTCTTGCTCA CTATCCCTCT CAAGGGTTTT CCACAGAGGA AGATGAAGAT 
GAACAAGTCT CTGCTGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014800197.1
CDS	115..5055
Translation	MASYVDNSFRQAVMKNPAERSPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPVPTHIAVSPSSTKGVIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSSSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014944711.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014944711.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGAGG 
AGCCCCCAGG ATTTGGAGAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCTAAT 
CTGAGAGAGC ATCAGCTAAG ACTGATGTGT GAAACTGTTA GATATGAAAG GCATGAAGCA 
AATGAAGTTC TGTACTACCC CGATGATATT GGTACCTGTT GGTATATCCT GCTGTCTGGT 
TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGGAGCA GCTTTGGCAA GCGTTCTGCA 
GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCCGAAAT GATTGTGGTG 
GATTACATGG ATGAAAATGA AGAGTATTTT CAACGTCAAG CTTCTCATAG GCAGTCTCGA 
AGAAGATTTA GGAAAATCAA CCAAAAAGGG GAACGACAAA CAATTATTGA TACTGTTGAC 
CCTTATCCTG TGGGGAAGCC ACCTCTACCT AGAGGCTACC ATACGCTTCC TGCAGATTTC 
ACAAAGCTGC ACCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC ATCTAGTCAC 
TCAGGATGTA GCATCACTAG TGACTCAGGA AGCAGCAGCC TTTCTGATAT CTACCAGGCT 
ACAGAAAGTG AGGCTGGTGA TATGGATCTC AGTGGGTTGC CAGAGACAGC AGTGGATTCT 
GAGGATGATG ATGATGAAGA AGACATTGAA AGGGCATCAG ATCCTTTGAT GAGTAGGGAT 
ATTGTTAGAG ACTGCTTGGA GAAAGACCCA ATAGACAGGA CAGATGATGA CATTGAACAA 
CTACTGGAAT TCATGCATCA ATTGCCTGCT TTTGCCAACA TGACAATGTC AGTGAGGAGA 
GAGCTCTGTG CAGTGATGGT GTTTGCAGTT GTGGAGAGAG CAGGAACTAT TGTACTAAAC 
GATGGGGAAG AGCTGGATTC CTGGTCAGTC ATTTTGAATG GGTCTGTGGA GGTGACATAC 
CCTGATGGAA GAACAGAGAT ACTCTGCATG GGGAACAGTT TTGGTGTTTC CCCTACCATG 
GAAAAGGAAT ATATGAAAGG AGTGATGAGA ACCAAAGTGG ATGACTGCCA GTTTGTTTGT 
ATAGCCCAGC AAGATTATTG CCGCATCCTC AATCAAGTGG AAAAGAACAT GCAGAAGGTA 
GAAGAGGAAG GGGAGATTGT GATGGTGAAA GAGCACAGGG AGCTTGATCG CACTGGAACA 
AGAAAAGGTC ACATTGTCAT CAAGGGAACA GCTGAAAGGT TAACAATGCA TTTGGTGGAA 
GAACATTCTG TGGTAGACCC AACATTTATA GAAGACTTCC TGTTGACATA TCGGACCTTT 
CTTTCCAGCC CAATGGAAGT GGGCAAGAAG TTGTTGGAGT GGTTCAATGA CCCCAGCCTC 
AGGGATAAGG TTACACGGGT AGTATTGTTG TGGGTGAACA ATCACTTCAA TGATTTTGAA 
GGAGATCCTG CTATGACTCG ATTTCTGGAA GAATTTGAGA ACAATTTGGA GAGGGAGAAA 
ATGGGTGGAC ATTTGAGGCT GTTAAATATT GCTTGTGCTG CTAAAGCTAA AAGAAGATTG 
ATAACCTTAA CAAAGCCATC TCGAGAAGCC CCTTTGCCTT TTATTTTGCT GGGAGGATCA 
GAAAAGGGAT TTGGAATCTT TGTTGACAGT GTCGATTTTG GTAGCAAAGC TACAGAAGCA 
GGCTTGAAAC GTGGAGACCA GATACTGGAA GTGAATGGTC AAAATTTTGA AAACATTCAG 
CTGTCAAAAG CCATGGAAAT CCTTAGAAAT AACACTCATC TGTCTATCAC TGTGAAAACC 
AATTTATTTG TTTTTAAAGA ACTCTTAACG CGGTTGTCAG AGGAAAAAAG AAATGGTGCT 
CCCCACCTGC CTAAAATTGG TGATATTAAG AAGGCGAGTC GTTATTCCAT CCCAGACCTT 
GCTGTTGATG TGGAGCAAGT TATAGGATTA GAAAAAGTAA ATAAGAAAAG TAAAGCCAAC 
ACAGTTGGGG GAAGAAATAA ATTAAAGAAG ATACTTGACA AGACTCGAAT CAGTATTCTG 
CCTCAGAAAC CCTACAATGA CATCGGAATT GGCCAGTCTC AGGATGACAG CATTGTGGGA 
CTGAGGCAAA CAAAGCACAT TCCTCCTGCT TTACCTGTCA GTGGAACCTT GTCATCCAGT 
AATCCTGATC TACTGCAGTC TCATCACCGC ATCTTAGACT TCAATACTAC TCCAGACTTA 
CCAGATCAAG TTCTGAGGGT TTTCAAGGCA GACCAGCAAA GTCGCTACAT CATGATCAGT 
AAGGACACAA CAGCGAAAGA AGTGGTCATC CAGGCCATCA GGGAGTTCGC TCTCACCACG 
ACCCCTGACG CGTATTCACT GTGTGAAGTC TCTGTCACAC CTGAGGGTGT CATCAAGCAA 
AGGAGGCTTC CTGATCAGTT ATCCAAGCTT GCTGATAGGA TACAGCTGAG TGGCAGGTAC 
TACCTGAAGA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAAGAGTTA 
CTACGAGAAA GTCAAATTTC CCTGCTACAG CTCAGTACTG TTGAGGTGGC TACCCAACTC 
TCCATGAGAA ACTTTGAACT ATTCCGTAAT ATTGAACCCA CGGAATACAT AGATGACTTG 
TTTAAACTCA AATCAAAAAC AGGTTGCACT AATCTAAAAA AGTTTGAAGA GGTGATAAAT 
CAAGAAACAT TTTGGGTGGC TTCTGAGATT CTAAGAGAAA CCAACCAGTT GAAAAGGATG 
AAGATCATTA AGCATTTCAT TAAGATAGCA CTGCACTGCA GAGAATGCAA GAACTTCAAC 
TCAATGTTTG CCATCATTAG TGGCCTGAAT TTGGCACCAG TTGCAAGGCT CCGAACTACT 
TGGGAAAAGC TTCCAAGCAA GTACGAGAAA CTGTTTCAAG ATCTACAGGA CTTGTTTGAT 
CCATCTAGGA ATATGGCAAA ATACCGTAAT GTCCTTAACA GCCAAAACCT GCAGCCCCCC 
ATTATCCCCC TGTTTCCTGT TATCAAAAAA GACCTTACGT TCCTTCATGA AGGAAATGAT 
TCAAAAGTAG AAGGCTTGGT CAATTTTGAG AAGCTGAGAA TGATTGCGAA GGAGATACGC 
CATGTTGGTC GCATGGCATC CGTGAACATG GATCCTGCTT TAATGTTTAG AACAAGGAAG 
AAGAAATGGA GGAGTTTGGG GTCTCTCAGC CAGGGCAGTA CGAACGCAGC GGTGCTGGAT 
GTTGCGCAAA CAGGGGGTCA CAAAAAGCGG GTCCGTCGCA GCTCCTTCCT TAATGCTAAA 
AAACTGTATG AAGATGCTCA AATGGCACGG AAGGTAAAGC AGTATCTCTC AAACTTGGAC 
CTGGAAATGG ATGAAGAGAG CCTCCAGACA CTGTCTCTGC AGTGTGAGCC AGCCACCAAC 
ACACTGCCGA AGAATACAGG AGACAAGCGA TCTGGAAAGT CCGAGACGTC ACCGGTGGCT 
CCCCGGGCAG GCATCCAGCA GAAAGTGCAG CAGCAGCAGC AACATAAAGT AAACCAAGCA 
TTGCAGGTCC CTGCTGTATC TCTGTATCCC TCTCGCAAGA AGGTGCCAGT CAAAGACCTC 
CCACCATTCG GCATTAACTC CCCACAAGCT TTAAAGAAAA TTCTTTCATT ATCTGAAGAA 
GGAAGTTTGG ATCGTCACAA GAAGCAATCT GAAGACACAG TCTCAAGTGC ATCTTCACAG 
CTGTCTTCTC CTCCCACTTC ACCACAGAGC TCTCCAAGGA AAGGCTATAC TTTGGCTCCT 
AGTGGCACGG TGGATAACTT CTCCGATTCT GGTCACAGTG AAATTTCTTC CAGATCAAGT 
ATTGTCAGCA ATTCCTCTTT TGACTCTATG CCGGTCTCCC TGCATGATGA GAGGAGACAG 
AGGCATTCTG TCAGCATTGT GGAGACAAAT CTTGGTGTGG GAAGGATTGA TAGAAGAATC 
ATGATTGAAC CAGATCAATA CAGCTTAGGC TCATATGCAC CATTGTCGGA GACCAGAGGC 
CTGTATGCTG GAGCAACGGT GCTTTCTTCT CCTAGTACAG AAGAACTGTC ACAGGACCAG 
GGGGACAGAG CTTCACTTGA TGCAGCTGAC AGTGGCCGTG GTAGCTGGAC TTCTTGTTCA 
AGTGGTTCTC ATGACAATAT ACAAACAATA CAGCACCAGA GAAGCTGGGA GACTCTGCCT 
TTTGGACACA CTCATTTCGA TAGTTCAGGC GATGGGGCAG GACTCTGGGC CTCAGGCAGT 
CATATGGACC AGCTGATGTT CCCCGATCAT GGCACAAAGT ATGGCAGGCA AAGTCAAGGT 
AGGGAGGGCC TTGATCAAGC ACAGTCCAGA GCAAGCTGGG CATCCTCAAC AGGATACTGG 
GGAGAGGACT CAGAAGGTGA TACAGGTACC ATAAAGCGGA GGGGTGGGAA GGATGTTTCA 
ATTGAAGCTG AAACCAGTAG CATAACATCT GTACCAGCAG AGGAGTCAAA GCCAGCTCCC 
GTGCCCACTC ATATAGCAGT ATCACCAAGT AGTACAAAGG GGGTTATTGC ACGAAAAGAG 
GGTCGATATC GGGAACCACC TCCAACTCCT CCTGGTTATG TAGGAATACC TATTGCTGAG 
TTTGCAGAAG GTGGCTCCCA TCCAACCAGA AAGCCTCCAG ATTACAACAT AGCACTTCAG 
AGGTCTAGAA TGGTGGCACG GACATGTGAG ACTCATGGGA CATCAACTCC GCAGCAGCAG 
TCACATGGCC ATTCATCTAG CAGGCCTGTG AACAAACCTC AGTGGCATAA ACCAAATGAG 
TCAGATCCAC GTCTTGCTCA CTATCCCTCT CAAGGGTTTT CCACAGAGGA AGATGAAGAT 
GAACAAGTCT CTGCTGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_014944719.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014800205.1 Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

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CloneID	OCa58506
Clone ID Related Accession (Same CDS sequence)	XM_014944719.1
Accession Version	XM_014944719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	rap guanine nucleotide exchange factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGAGG 
AGCCCCCAGG ATTTGGAGAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCTAAT 
CTGAGAGAGC ATCAGCTAAG ACTGATGTGT GAAACTGTTA GATATGAAAG GCATGAAGCA 
AATGAAGTTC TGTACTACCC CGATGATATT GGTACCTGTT GGTATATCCT GCTGTCTGGT 
TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGGAGCA GCTTTGGCAA GCGTTCTGCA 
GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCCGAAAT GATTGTGGTG 
GATTACATGG ATGAAAATGA AGAGTATTTT CAACGTCAAG CTTCTCATAG GCAGTCTCGA 
AGAAGATTTA GGAAAATCAA CCAAAAAGGG GAACGACAAA CAATTATTGA TACTGTTGAC 
CCTTATCCTG TGGGGAAGCC ACCTCTACCT AGAGGCTACC ATACGGAATG CACTAAACCT 
CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCAT CTAGTCACTC AGGATGTAGC ATCACTAGTG ACTCAGGAAG CAGCAGCCTT 
TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT 
CCTTTGATGA GTAGGGATAT TGTTAGAGAC TGCTTGGAGA AAGACCCAAT AGACAGGACA 
GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAAT TGCCTGCTTT TGCCAACATG 
ACAATGTCAG TGAGGAGAGA GCTCTGTGCA GTGATGGTGT TTGCAGTTGT GGAGAGAGCA 
GGAACTATTG TACTAAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TTTGAATGGG 
TCTGTGGAGG TGACATACCC TGATGGAAGA ACAGAGATAC TCTGCATGGG GAACAGTTTT 
GGTGTTTCCC CTACCATGGA AAAGGAATAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GACTGCCAGT TTGTTTGTAT AGCCCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAGAACATGC AGAAGGTAGA AGAGGAAGGG GAGATTGTGA TGGTGAAAGA GCACAGGGAG 
CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC TGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG 
TTGACATATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GCAAGAAGTT GTTGGAGTGG 
TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC 
AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGTGCTGCT 
AAAGCTAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT 
ATTTTGCTGG GAGGATCAGA AAAGGGATTT GGAATCTTTG TTGACAGTGT CGATTTTGGT 
AGCAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TACTGGAAGT GAATGGTCAA 
AATTTTGAAA ACATTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG 
TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TCTTAACGCG GTTGTCAGAG 
GAAAAAAGAA ATGGTGCTCC CCACCTGCCT AAAATTGGTG ATATTAAGAA GGCGAGTCGT 
TATTCCATCC CAGACCTTGC TGTTGATGTG GAGCAAGTTA TAGGATTAGA AAAAGTAAAT 
AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAAT TAAAGAAGAT ACTTGACAAG 
ACTCGAATCA GTATTCTGCC TCAGAAACCC TACAATGACA TCGGAATTGG CCAGTCTCAG 
GATGACAGCA TTGTGGGACT GAGGCAAACA AAGCACATTC CTCCTGCTTT ACCTGTCAGT 
GGAACCTTGT CATCCAGTAA TCCTGATCTA CTGCAGTCTC ATCACCGCAT CTTAGACTTC 
AATACTACTC CAGACTTACC AGATCAAGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGT 
CGCTACATCA TGATCAGTAA GGACACAACA GCGAAAGAAG TGGTCATCCA GGCCATCAGG 
GAGTTCGCTC TCACCACGAC CCCTGACGCG TATTCACTGT GTGAAGTCTC TGTCACACCT 
GAGGGTGTCA TCAAGCAAAG GAGGCTTCCT GATCAGTTAT CCAAGCTTGC TGATAGGATA 
CAGCTGAGTG GCAGGTACTA CCTGAAGAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AAGAGTTACT ACGAGAAAGT CAAATTTCCC TGCTACAGCT CAGTACTGTT 
GAGGTGGCTA CCCAACTCTC CATGAGAAAC TTTGAACTAT TCCGTAATAT TGAACCCACG 
GAATACATAG ATGACTTGTT TAAACTCAAA TCAAAAACAG GTTGCACTAA TCTAAAAAAG 
TTTGAAGAGG TGATAAATCA AGAAACATTT TGGGTGGCTT CTGAGATTCT AAGAGAAACC 
AACCAGTTGA AAAGGATGAA GATCATTAAG CATTTCATTA AGATAGCACT GCACTGCAGA 
GAATGCAAGA ACTTCAACTC AATGTTTGCC ATCATTAGTG GCCTGAATTT GGCACCAGTT 
GCAAGGCTCC GAACTACTTG GGAAAAGCTT CCAAGCAAGT ACGAGAAACT GTTTCAAGAT 
CTACAGGACT TGTTTGATCC ATCTAGGAAT ATGGCAAAAT ACCGTAATGT CCTTAACAGC 
CAAAACCTGC AGCCCCCCAT TATCCCCCTG TTTCCTGTTA TCAAAAAAGA CCTTACGTTC 
CTTCATGAAG GAAATGATTC AAAAGTAGAA GGCTTGGTCA ATTTTGAGAA GCTGAGAATG 
ATTGCGAAGG AGATACGCCA TGTTGGTCGC ATGGCATCCG TGAACATGGA TCCTGCTTTA 
ATGTTTAGAA CAAGGAAGAA GAAATGGAGG AGTTTGGGGT CTCTCAGCCA GGGCAGTACG 
AACGCAGCGG TGCTGGATGT TGCGCAAACA GGGGGTCACA AAAAGCGGGT CCGTCGCAGC 
TCCTTCCTTA ATGCTAAAAA ACTGTATGAA GATGCTCAAA TGGCACGGAA GGTAAAGCAG 
TATCTCTCAA ACTTGGACCT GGAAATGGAT GAAGAGAGCC TCCAGACACT GTCTCTGCAG 
TGTGAGCCAG CCACCAACAC ACTGCCGAAG AATACAGGAG ACAAGCGATC TGGAAAGTCC 
GAGACGTCAC CGGTGGCTCC CCGGGCAGGC ATCCAGCAGA AAGTGCAGCA GCAGCAGCAA 
CATAAAGTAA ACCAAGCATT GCAGGTCCCT GCTGTATCTC TGTATCCCTC TCGCAAGAAG 
GTGCCAGTCA AAGACCTCCC ACCATTCGGC ATTAACTCCC CACAAGCTTT AAAGAAAATT 
CTTTCATTAT CTGAAGAAGG AAGTTTGGAT CGTCACAAGA AGCAATCTGA AGACACAGTC 
TCAAGTGCAT CTTCACAGCT GTCTTCTCCT CCCACTTCAC CACAGAGCTC TCCAAGGAAA 
GGCTATACTT TGGCTCCTAG TGGCACGGTG GATAACTTCT CCGATTCTGG TCACAGTGAA 
ATTTCTTCCA GATCAAGTAT TGTCAGCAAT TCCTCTTTTG ACTCTATGCC GGTCTCCCTG 
CATGATGAGA GGAGACAGAG GCATTCTGTC AGCATTGTGG AGACAAATCT TGGTGTGGGA 
AGGATTGATA GAAGAATCAT GATTGAACCA GATCAATACA GCTTAGGCTC ATATGCACCA 
TTGTCGGAGA CCAGAGGCCT GTATGCTGGA GCAACGGTGC TTTCTTCTCC TAGTACAGAA 
GAACTGTCAC AGGACCAGGG GGACAGAGCT TCACTTGATG CAGCTGACAG TGGCCGTGGT 
AGCTGGACTT CTTGTTCAAG TGGTTCTCAT GACAATATAC AAACAATACA GCACCAGAGA 
AGCTGGGAGA CTCTGCCTTT TGGACACACT CATTTCGATA GTTCAGGCGA TGGGGCAGGA 
CTCTGGGCCT CAGGCAGTCA TATGGACCAG CTGATGTTCC CCGATCATGG CACAAAGTAT 
GGCAGGCAAA GTCAAGGTAG GGAGGGCCTT GATCAAGCAC AGTCCAGAGC AAGCTGGGCA 
TCCTCAACAG GATACTGGGG AGAGGACTCA GAAGGTGATA CAGGTACCAT AAAGCGGAGG 
GGTGGGAAGG ATGTTTCAAT TGAAGCTGAA ACCAGTAGCA TAACATCTGT ACCAGCAGAG 
GAGTCAAAGC CAGCTCCCGT GCCCACTCAT ATAGCAGTAT CACCAAGTAG TACAAAGGGG 
GTTATTGCAC GAAAAGAGGG TCGATATCGG GAACCACCTC CAACTCCTCC TGGTTATGTA 
GGAATACCTA TTGCTGAGTT TGCAGAAGGT GGCTCCCATC CAACCAGAAA GCCTCCAGAT 
TACAACATAG CACTTCAGAG GTCTAGAATG GTGGCACGGA CATGTGAGAC TCATGGGACA 
TCAACTCCGC AGCAGCAGTC ACATGGCCAT TCATCTAGCA GGCCTGTGAA CAAACCTCAG 
TGGCATAAAC CAAATGAGTC AGATCCACGT CTTGCTCACT ATCCCTCTCA AGGGTTTTCC 
ACAGAGGAAG ATGAAGATGA ACAAGTCTCT GCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014800205.1
CDS	115..5073
Translation	MASYVDNSFRQAVMKNPAERSPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKPQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTTTPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPVPTHIAVSPSSTKGVIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSSSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_014944719.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014944719.1

ORF Insert Sequence:

ATGGCGTCCT ACGTGGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAACCC GGCGGAGAGG 
AGCCCCCAGG ATTTGGAGAT AGTGTATTCC TATTTACATG GAATGGAAGC CTTGTCTAAT 
CTGAGAGAGC ATCAGCTAAG ACTGATGTGT GAAACTGTTA GATATGAAAG GCATGAAGCA 
AATGAAGTTC TGTACTACCC CGATGATATT GGTACCTGTT GGTATATCCT GCTGTCTGGT 
TCAGTCTTCA TTAAAGAATC TATGTTTCTT CCAAGGAGCA GCTTTGGCAA GCGTTCTGCA 
GGAAGCTTCA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CATCCGAAAT GATTGTGGTG 
GATTACATGG ATGAAAATGA AGAGTATTTT CAACGTCAAG CTTCTCATAG GCAGTCTCGA 
AGAAGATTTA GGAAAATCAA CCAAAAAGGG GAACGACAAA CAATTATTGA TACTGTTGAC 
CCTTATCCTG TGGGGAAGCC ACCTCTACCT AGAGGCTACC ATACGGAATG CACTAAACCT 
CAGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCAT CTAGTCACTC AGGATGTAGC ATCACTAGTG ACTCAGGAAG CAGCAGCCTT 
TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTCAG TGGGTTGCCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCAGAT 
CCTTTGATGA GTAGGGATAT TGTTAGAGAC TGCTTGGAGA AAGACCCAAT AGACAGGACA 
GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAAT TGCCTGCTTT TGCCAACATG 
ACAATGTCAG TGAGGAGAGA GCTCTGTGCA GTGATGGTGT TTGCAGTTGT GGAGAGAGCA 
GGAACTATTG TACTAAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TTTGAATGGG 
TCTGTGGAGG TGACATACCC TGATGGAAGA ACAGAGATAC TCTGCATGGG GAACAGTTTT 
GGTGTTTCCC CTACCATGGA AAAGGAATAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GACTGCCAGT TTGTTTGTAT AGCCCAGCAA GATTATTGCC GCATCCTCAA TCAAGTGGAA 
AAGAACATGC AGAAGGTAGA AGAGGAAGGG GAGATTGTGA TGGTGAAAGA GCACAGGGAG 
CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC TGAAAGGTTA 
ACAATGCATT TGGTGGAAGA ACATTCTGTG GTAGACCCAA CATTTATAGA AGACTTCCTG 
TTGACATATC GGACCTTTCT TTCCAGCCCA ATGGAAGTGG GCAAGAAGTT GTTGGAGTGG 
TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTCTGGAAGA ATTTGAGAAC 
AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGTGCTGCT 
AAAGCTAAAA GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT 
ATTTTGCTGG GAGGATCAGA AAAGGGATTT GGAATCTTTG TTGACAGTGT CGATTTTGGT 
AGCAAAGCTA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TACTGGAAGT GAATGGTCAA 
AATTTTGAAA ACATTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG 
TCTATCACTG TGAAAACCAA TTTATTTGTT TTTAAAGAAC TCTTAACGCG GTTGTCAGAG 
GAAAAAAGAA ATGGTGCTCC CCACCTGCCT AAAATTGGTG ATATTAAGAA GGCGAGTCGT 
TATTCCATCC CAGACCTTGC TGTTGATGTG GAGCAAGTTA TAGGATTAGA AAAAGTAAAT 
AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAAT TAAAGAAGAT ACTTGACAAG 
ACTCGAATCA GTATTCTGCC TCAGAAACCC TACAATGACA TCGGAATTGG CCAGTCTCAG 
GATGACAGCA TTGTGGGACT GAGGCAAACA AAGCACATTC CTCCTGCTTT ACCTGTCAGT 
GGAACCTTGT CATCCAGTAA TCCTGATCTA CTGCAGTCTC ATCACCGCAT CTTAGACTTC 
AATACTACTC CAGACTTACC AGATCAAGTT CTGAGGGTTT TCAAGGCAGA CCAGCAAAGT 
CGCTACATCA TGATCAGTAA GGACACAACA GCGAAAGAAG TGGTCATCCA GGCCATCAGG 
GAGTTCGCTC TCACCACGAC CCCTGACGCG TATTCACTGT GTGAAGTCTC TGTCACACCT 
GAGGGTGTCA TCAAGCAAAG GAGGCTTCCT GATCAGTTAT CCAAGCTTGC TGATAGGATA 
CAGCTGAGTG GCAGGTACTA CCTGAAGAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AAGAGTTACT ACGAGAAAGT CAAATTTCCC TGCTACAGCT CAGTACTGTT 
GAGGTGGCTA CCCAACTCTC CATGAGAAAC TTTGAACTAT TCCGTAATAT TGAACCCACG 
GAATACATAG ATGACTTGTT TAAACTCAAA TCAAAAACAG GTTGCACTAA TCTAAAAAAG 
TTTGAAGAGG TGATAAATCA AGAAACATTT TGGGTGGCTT CTGAGATTCT AAGAGAAACC 
AACCAGTTGA AAAGGATGAA GATCATTAAG CATTTCATTA AGATAGCACT GCACTGCAGA 
GAATGCAAGA ACTTCAACTC AATGTTTGCC ATCATTAGTG GCCTGAATTT GGCACCAGTT 
GCAAGGCTCC GAACTACTTG GGAAAAGCTT CCAAGCAAGT ACGAGAAACT GTTTCAAGAT 
CTACAGGACT TGTTTGATCC ATCTAGGAAT ATGGCAAAAT ACCGTAATGT CCTTAACAGC 
CAAAACCTGC AGCCCCCCAT TATCCCCCTG TTTCCTGTTA TCAAAAAAGA CCTTACGTTC 
CTTCATGAAG GAAATGATTC AAAAGTAGAA GGCTTGGTCA ATTTTGAGAA GCTGAGAATG 
ATTGCGAAGG AGATACGCCA TGTTGGTCGC ATGGCATCCG TGAACATGGA TCCTGCTTTA 
ATGTTTAGAA CAAGGAAGAA GAAATGGAGG AGTTTGGGGT CTCTCAGCCA GGGCAGTACG 
AACGCAGCGG TGCTGGATGT TGCGCAAACA GGGGGTCACA AAAAGCGGGT CCGTCGCAGC 
TCCTTCCTTA ATGCTAAAAA ACTGTATGAA GATGCTCAAA TGGCACGGAA GGTAAAGCAG 
TATCTCTCAA ACTTGGACCT GGAAATGGAT GAAGAGAGCC TCCAGACACT GTCTCTGCAG 
TGTGAGCCAG CCACCAACAC ACTGCCGAAG AATACAGGAG ACAAGCGATC TGGAAAGTCC 
GAGACGTCAC CGGTGGCTCC CCGGGCAGGC ATCCAGCAGA AAGTGCAGCA GCAGCAGCAA 
CATAAAGTAA ACCAAGCATT GCAGGTCCCT GCTGTATCTC TGTATCCCTC TCGCAAGAAG 
GTGCCAGTCA AAGACCTCCC ACCATTCGGC ATTAACTCCC CACAAGCTTT AAAGAAAATT 
CTTTCATTAT CTGAAGAAGG AAGTTTGGAT CGTCACAAGA AGCAATCTGA AGACACAGTC 
TCAAGTGCAT CTTCACAGCT GTCTTCTCCT CCCACTTCAC CACAGAGCTC TCCAAGGAAA 
GGCTATACTT TGGCTCCTAG TGGCACGGTG GATAACTTCT CCGATTCTGG TCACAGTGAA 
ATTTCTTCCA GATCAAGTAT TGTCAGCAAT TCCTCTTTTG ACTCTATGCC GGTCTCCCTG 
CATGATGAGA GGAGACAGAG GCATTCTGTC AGCATTGTGG AGACAAATCT TGGTGTGGGA 
AGGATTGATA GAAGAATCAT GATTGAACCA GATCAATACA GCTTAGGCTC ATATGCACCA 
TTGTCGGAGA CCAGAGGCCT GTATGCTGGA GCAACGGTGC TTTCTTCTCC TAGTACAGAA 
GAACTGTCAC AGGACCAGGG GGACAGAGCT TCACTTGATG CAGCTGACAG TGGCCGTGGT 
AGCTGGACTT CTTGTTCAAG TGGTTCTCAT GACAATATAC AAACAATACA GCACCAGAGA 
AGCTGGGAGA CTCTGCCTTT TGGACACACT CATTTCGATA GTTCAGGCGA TGGGGCAGGA 
CTCTGGGCCT CAGGCAGTCA TATGGACCAG CTGATGTTCC CCGATCATGG CACAAAGTAT 
GGCAGGCAAA GTCAAGGTAG GGAGGGCCTT GATCAAGCAC AGTCCAGAGC AAGCTGGGCA 
TCCTCAACAG GATACTGGGG AGAGGACTCA GAAGGTGATA CAGGTACCAT AAAGCGGAGG 
GGTGGGAAGG ATGTTTCAAT TGAAGCTGAA ACCAGTAGCA TAACATCTGT ACCAGCAGAG 
GAGTCAAAGC CAGCTCCCGT GCCCACTCAT ATAGCAGTAT CACCAAGTAG TACAAAGGGG 
GTTATTGCAC GAAAAGAGGG TCGATATCGG GAACCACCTC CAACTCCTCC TGGTTATGTA 
GGAATACCTA TTGCTGAGTT TGCAGAAGGT GGCTCCCATC CAACCAGAAA GCCTCCAGAT 
TACAACATAG CACTTCAGAG GTCTAGAATG GTGGCACGGA CATGTGAGAC TCATGGGACA 
TCAACTCCGC AGCAGCAGTC ACATGGCCAT TCATCTAGCA GGCCTGTGAA CAAACCTCAG 
TGGCATAAAC CAAATGAGTC AGATCCACGT CTTGCTCACT ATCCCTCTCA AGGGTTTTCC 
ACAGAGGAAG ATGAAGATGA ACAAGTCTCT GCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RAPGEF2 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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