ORF » Species Summary » Calidris pugnax » KCNMA1 cDNA ORF clone

KCNMA1 cDNA ORF clone, Calidris pugnax(ruff)

The following KCNMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa75476 XM_014956256.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75477 XM_014956257.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75478 XM_014956258.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75479 XM_014956259.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75480 XM_014956260.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75481 XM_014956261.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75482 XM_014956263.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75483 XM_014956264.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75484 XM_014956265.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75485 XM_014956266.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75486 XM_014956267.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75487 XM_014956268.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75488 XM_014956269.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75489 XM_014956270.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75490 XM_014956271.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75491 XM_014956272.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa75492 XM_014956274.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa75493 XM_014956275.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa75494 XM_014956276.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCa75495 XM_014956277.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75496 XM_014956278.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75497 XM_014956279.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75498 XM_014956280.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCa75499 XM_014956281.1
Latest version!		 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    KCNMA1 ( XM_014956256.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811742.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCa75476
    Clone ID Related Accession (Same CDS sequence) XM_014956256.1
    Accession Version XM_014956256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT 
    CAGGAAAAAA ACAGTGGAAC CCACATTCTA ATGATAACTG AACTGGTAAA TGATTCAAAT 
    GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGACA CAGAACTGTA TCTTACGCAG 
    CCCTTTGCAT GTGGAACTGC ATTTGCTGTC AGTGTGCTGG ACTCTCTCAT GAGCGCAACA 
    TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA 
    GAGCTGGAAG CACTGATTGC TGAGGAAAAT GCGTTGAGAG GAGGTTACAG CACGCCCCAG 
    ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGTTGG CTTTGTATGA TGGGCCGTTC 
    GCAGACCTAG GGGACGGAGG TTGTTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC 
    AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGATGCAC ATTTAAGTAC TCCCAGCCAA 
    TGCACTAAAC GTTATGTTAT CACAAACCCA CCGTATGAAT TTGAGCTTGT GCCGACAGAC 
    TTGATCTTCT GTTTGATGCA ATTTGACCAC AACGCTGGCC AGTCCCGGGC CAGTCTTTCT 
    CATTCCTCCC ATTCCTCCTA TTCTTCCAGT AAGAAGAGCT CATCAGTTCA CTCCATCCCA 
    TCAACAGCAA ACCGACCGAA CCGCCCCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA 
    ACAAGGATGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT 
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACTC ACATGGCCAA TCAGATCAAT 
    CAATATAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811742.1
    CDS1..3840
    Translation

    Target ORF information:

    RefSeq Version XM_014956256.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT 
    CAGGAAAAAA ACAGTGGAAC CCACATTCTA ATGATAACTG AACTGGTAAA TGATTCAAAT 
    GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGACA CAGAACTGTA TCTTACGCAG 
    CCCTTTGCAT GTGGAACTGC ATTTGCTGTC AGTGTGCTGG ACTCTCTCAT GAGCGCAACA 
    TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA 
    GAGCTGGAAG CACTGATTGC TGAGGAAAAT GCGTTGAGAG GAGGTTACAG CACGCCCCAG 
    ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGTTGG CTTTGTATGA TGGGCCGTTC 
    GCAGACCTAG GGGACGGAGG TTGTTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC 
    AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGATGCAC ATTTAAGTAC TCCCAGCCAA 
    TGCACTAAAC GTTATGTTAT CACAAACCCA CCGTATGAAT TTGAGCTTGT GCCGACAGAC 
    TTGATCTTCT GTTTGATGCA ATTTGACCAC AACGCTGGCC AGTCCCGGGC CAGTCTTTCT 
    CATTCCTCCC ATTCCTCCTA TTCTTCCAGT AAGAAGAGCT CATCAGTTCA CTCCATCCCA 
    TCAACAGCAA ACCGACCGAA CCGCCCCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA 
    ACAAGGATGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT TTACAGATGA ACCGGATAAT 
    GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACTC ACATGGCCAA TCAGATCAAT 
    CAATATAAAT CGACAAGCAG CTTGATACCA CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956257.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811743.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCa75477
    Clone ID Related Accession (Same CDS sequence) XM_014956257.1
    Accession Version XM_014956257.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCGC TCATCATCCC GGTGACCATG GAGGTGCCCT GCGATAGCCG CGGACAGCGC 
    ATGTGGTGGG CTTTCCTCGC CTCATCCATG GTCACTTTCT TTGGGGGACT GTTTATCATC 
    CTGCTGTGGA GGACCCTCAA GTACCTGTGG ACTGTCTGCT GCCACTGCGG GGTCAAAAAC 
    AAGGAAGCCC AGAAGATCAA TGGTGGTGGA GATACACAGG CAGATGGGGC CTGCAAACCA 
    ACAGATGAAA AAGAGGAGAA TGTGGCAGCA GAAGTGGGGT GGATGACCTC TGTGAAGGAT 
    TGGGCAGGAG TCATGATATC TGCCCAGACA TTAACTGGCA GAGTACTTGT TGTCTTAGTC 
    TTCGCTCTTA GTATTGGTGC TCTTGTAATA TACTTCATAG ATTCATCAAA CCCAATAGAA 
    TCCTGCCAGA ATTTCTACAA AGATTTCACG TTACAGATTG ACATGGCTTT CAATGTGTTC 
    TTCCTACTCT ACTTCGGCTT GCGTTTCATA GCAGCCAATG ACAAGCTATG GTTTTGGCTA 
    GAAGTTAACT CAGTAGTAGA TTTCTTCACG GTCCCTCCAG TGTTTGTGTC AGTATATTTA 
    AACAGAAGCT GGCTTGGTTT AAGATTTTTA AGAGCTCTTA GACTGATACA ATTTTCAGAA 
    ATTTTGCAGT TTCTGAATAT CCTTAAAACA AGTAACTCCA TCAAGCTAGT GAATCTGTGC 
    TCTATATTTA TCAGCACGTG GCTGACAGCA GCTGGGTTCA TACATTTGGT GGAGAACTCA 
    GGGGATCCAT GGGAAAATTT CCAAAATAAT CAACCGCTAA CATATTGGGA ATGTGTTTAT 
    TTACTGATGG TTACAATGTC CACTGTTGGC TATGGAGATG TTTATGCAAA AACAACCCTT 
    GGTCGCCTTT TCATGGTCTT CTTCATCCTT GGGGGATTGG CCATGTTTGC CAGCTACGTC 
    CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATATG GTGGTTCCTA TAGTGCGGTT 
    AGTGGAAGAA AGCACATAGT TGTCTGTGGT CACATAACAC TTGAAAGTGT GTCCAACTTC 
    CTGAAGGACT TCCTGCACAA GGATAGGGAT GATGTCAACG TAGAAATCGT CTTTCTGCAT 
    AATATCTCCC CTAACCTTGA GCTGGAAGCT CTTTTTAAAC GACACTTCAC TCAGGTGGAA 
    TTTTATCAGG GTTCAGTCCT TAATCCCCAT GACCTGGCGA GAGTGAAGAT AGAGTCAGCA 
    GATGCGTGCC TAATCCTTGC CAATAAATAC TGCGCTGACC CAGATGCTGA AGATGCCTCC 
    AATATTATGA GAGTTATTTC AATAAAGAAT TATCATCCGA AGATAAGAAT TATCACTCAG 
    ATGTTGCAGT ATCACAATAA GGCTCATCTG CTAAATATCC CAAGCTGGAA CTGGAAAGAA 
    GGGGATGATG CAATCTGTCT TGCTGAGCTC AAGCTGGGTT TCATAGCCCA GAGCTGCCTG 
    GCACCGGGCC TTTCAACAAT GTTAGCCAAC CTCTTTTCTA TGAGGTCATT CATAAAGATT 
    GAGGAGGATA CGTGGCAGAA ATACTACCTG GAAGGAGTTG CAAATGAAAT GTATACAGAA 
    TATCTGTCTA GTGCCTTTGT GGGTTTGTCC TTCCCTGCAG TTTGTGAACT GGTGTTTGCG 
    AAACTAAAGC TCTTAATGAT AGCCATAGAA TACAAGTCGG AAAAAAGAGA AAGCAGAAGC 
    CGAAAGCGTA TATTAATCAA TCCTGGAAAC CATGTCAAGA TTCAAGAAGG TACTTTAGGA 
    TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT TTTTTTACTG CAAGGCCTGT 
    CATGATGACA TCACGGATCC CAAAAGAATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
    TCCAAGATGT CCATCTACAA GAGAATGAAA CTGGCATGTT GTTTTGATTG CGGACGCTCT 
    GAGCGTGACT GCTCTTGCAT GTCAGGCAGT GTACATAGTA ACATGGACAC CCTTGAGAGA 
    GCCTTCCCAC TTTCTTCTGT CTCTGTTAAT GATTGCTCCA CCAGTTTACG TGCCTTTGAA 
    GATGAGCAGC CGTCGACACT ATCACCCAAA AAAAAGCAGC GAAACGGAGG CATGCGAAAT 
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAACGAG 
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACAGGGAT GTTTCATTGG 
    TGTCCAGCCA AGGACATCGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT 
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA 
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT 
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACACTGCA TAACTTCCCC 
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC 
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG 
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC 
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGTTTTACAC CCCCTGGGAT GGATAGGTCA 
    TCTCCAGACA ACAGTCCAGT CCATGGCCTG TTACGCCAAC CATCCATTAC AACAGGAGCC 
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC 
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA 
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ACACAGAACT GTATCTTACG 
    CAGCCCTTTG CATGTGGAAC TGCATTTGCT GTCAGTGTGC TGGACTCTCT CATGAGCGCA 
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA 
    CCAGAGCTGG AAGCACTGAT TGCTGAGGAA AATGCGTTGA GAGGAGGTTA CAGCACGCCC 
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCG 
    TTCGCAGACC TAGGGGACGG AGGTTGTTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA 
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGATG CACATTTAAG TACTCCCAGC 
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCGTATG AATTTGAGCT TGTGCCGACA 
    GACTTGATCT TCTGTTTGAT GCAATTTGAC CACAACGCTG GCCAGTCCCG GGCCAGTCTT 
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGTAAGAAGA GCTCATCAGT TCACTCCATC 
    CCATCAACAG CAAACCGACC GAACCGCCCC AAGACCAGGG ATTCCCGGGA AAAACAGAAT 
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT 
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CTCACATGGC CAATCAGATC 
    AATCAATATA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811743.1
    CDS285..4067
    Translation

    Target ORF information:

    RefSeq Version XM_014956257.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956257.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGCGC TCATCATCCC GGTGACCATG GAGGTGCCCT GCGATAGCCG CGGACAGCGC 
    ATGTGGTGGG CTTTCCTCGC CTCATCCATG GTCACTTTCT TTGGGGGACT GTTTATCATC 
    CTGCTGTGGA GGACCCTCAA GTACCTGTGG ACTGTCTGCT GCCACTGCGG GGTCAAAAAC 
    AAGGAAGCCC AGAAGATCAA TGGTGGTGGA GATACACAGG CAGATGGGGC CTGCAAACCA 
    ACAGATGAAA AAGAGGAGAA TGTGGCAGCA GAAGTGGGGT GGATGACCTC TGTGAAGGAT 
    TGGGCAGGAG TCATGATATC TGCCCAGACA TTAACTGGCA GAGTACTTGT TGTCTTAGTC 
    TTCGCTCTTA GTATTGGTGC TCTTGTAATA TACTTCATAG ATTCATCAAA CCCAATAGAA 
    TCCTGCCAGA ATTTCTACAA AGATTTCACG TTACAGATTG ACATGGCTTT CAATGTGTTC 
    TTCCTACTCT ACTTCGGCTT GCGTTTCATA GCAGCCAATG ACAAGCTATG GTTTTGGCTA 
    GAAGTTAACT CAGTAGTAGA TTTCTTCACG GTCCCTCCAG TGTTTGTGTC AGTATATTTA 
    AACAGAAGCT GGCTTGGTTT AAGATTTTTA AGAGCTCTTA GACTGATACA ATTTTCAGAA 
    ATTTTGCAGT TTCTGAATAT CCTTAAAACA AGTAACTCCA TCAAGCTAGT GAATCTGTGC 
    TCTATATTTA TCAGCACGTG GCTGACAGCA GCTGGGTTCA TACATTTGGT GGAGAACTCA 
    GGGGATCCAT GGGAAAATTT CCAAAATAAT CAACCGCTAA CATATTGGGA ATGTGTTTAT 
    TTACTGATGG TTACAATGTC CACTGTTGGC TATGGAGATG TTTATGCAAA AACAACCCTT 
    GGTCGCCTTT TCATGGTCTT CTTCATCCTT GGGGGATTGG CCATGTTTGC CAGCTACGTC 
    CCTGAAATCA TAGAGTTAAT AGGAAACCGC AAGAAATATG GTGGTTCCTA TAGTGCGGTT 
    AGTGGAAGAA AGCACATAGT TGTCTGTGGT CACATAACAC TTGAAAGTGT GTCCAACTTC 
    CTGAAGGACT TCCTGCACAA GGATAGGGAT GATGTCAACG TAGAAATCGT CTTTCTGCAT 
    AATATCTCCC CTAACCTTGA GCTGGAAGCT CTTTTTAAAC GACACTTCAC TCAGGTGGAA 
    TTTTATCAGG GTTCAGTCCT TAATCCCCAT GACCTGGCGA GAGTGAAGAT AGAGTCAGCA 
    GATGCGTGCC TAATCCTTGC CAATAAATAC TGCGCTGACC CAGATGCTGA AGATGCCTCC 
    AATATTATGA GAGTTATTTC AATAAAGAAT TATCATCCGA AGATAAGAAT TATCACTCAG 
    ATGTTGCAGT ATCACAATAA GGCTCATCTG CTAAATATCC CAAGCTGGAA CTGGAAAGAA 
    GGGGATGATG CAATCTGTCT TGCTGAGCTC AAGCTGGGTT TCATAGCCCA GAGCTGCCTG 
    GCACCGGGCC TTTCAACAAT GTTAGCCAAC CTCTTTTCTA TGAGGTCATT CATAAAGATT 
    GAGGAGGATA CGTGGCAGAA ATACTACCTG GAAGGAGTTG CAAATGAAAT GTATACAGAA 
    TATCTGTCTA GTGCCTTTGT GGGTTTGTCC TTCCCTGCAG TTTGTGAACT GGTGTTTGCG 
    AAACTAAAGC TCTTAATGAT AGCCATAGAA TACAAGTCGG AAAAAAGAGA AAGCAGAAGC 
    CGAAAGCGTA TATTAATCAA TCCTGGAAAC CATGTCAAGA TTCAAGAAGG TACTTTAGGA 
    TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT TTTTTTACTG CAAGGCCTGT 
    CATGATGACA TCACGGATCC CAAAAGAATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
    TCCAAGATGT CCATCTACAA GAGAATGAAA CTGGCATGTT GTTTTGATTG CGGACGCTCT 
    GAGCGTGACT GCTCTTGCAT GTCAGGCAGT GTACATAGTA ACATGGACAC CCTTGAGAGA 
    GCCTTCCCAC TTTCTTCTGT CTCTGTTAAT GATTGCTCCA CCAGTTTACG TGCCTTTGAA 
    GATGAGCAGC CGTCGACACT ATCACCCAAA AAAAAGCAGC GAAACGGAGG CATGCGAAAT 
    TCACCCAACT CCTCACCCAA ACTGATGAGG CATGACCCCT TGTTAATTCC TGGCAACGAG 
    CAGATTGACA ATATGGATGC CAATGTGAAA AAATATGACT CTACAGGGAT GTTTCATTGG 
    TGTCCAGCCA AGGACATCGA AAAGGTCATT TTGACTCGAA GTGAAGCAGC GATGACAGTT 
    TTAAGTGGGC ATGTAGTCGT TTGCATATTT GGGGATGTTA AATCTGCTTT GATTGGATTA 
    AGAAATTTAG TGATGCCATT ACGAGCCAGC AACTTTCACT ACCATGAACT CAAGCACATT 
    GTGTTTGTGG GTTCACTTGA ATACCTGAGA AGGGAATGGG AGACACTGCA TAACTTCCCC 
    AAGGTTTCAA TCTTGCCTGG TACACCATTA AGTCGGGCTG ATTTAAGGGC TGTCAACATC 
    AACCTCTGCG ACATGTGCGT TATCCTGTCA GCCAATCAGA ATAATATTGA TGATGCTTCG 
    CTGCAGGACA AGGAATGCAT CTTGGCGTCA CTCAACATCA AATCTATGCA GTTTGATGAC 
    AGCATTGGGG TCTTGCAGGC TAATTCCCAA GGTTTTACAC CCCCTGGGAT GGATAGGTCA 
    TCTCCAGACA ACAGTCCAGT CCATGGCCTG TTACGCCAAC CATCCATTAC AACAGGAGCC 
    AACATCCCTA TTATAACAGA GCTAGCTAAG CCTGGCAAAC TTCTGCCTTT GGTTTCAATC 
    AGTCAGGAAA AAAACAGTGG AACCCACATT CTAATGATAA CTGAACTGGT AAATGATTCA 
    AATGTTCAGT TCTTGGACCA AGATGATGAT GATGATCCAG ACACAGAACT GTATCTTACG 
    CAGCCCTTTG CATGTGGAAC TGCATTTGCT GTCAGTGTGC TGGACTCTCT CATGAGCGCA 
    ACATACTTCA ATGACAATAT CCTCACACTG ATACGGACAT TGGTAACAGG AGGAGCCACA 
    CCAGAGCTGG AAGCACTGAT TGCTGAGGAA AATGCGTTGA GAGGAGGTTA CAGCACGCCC 
    CAGACACTTG CAAACAGGGA CAGGTGTCGA GTTGCTCAGT TGGCTTTGTA TGATGGGCCG 
    TTCGCAGACC TAGGGGACGG AGGTTGTTAT GGAGATCTAT TTTGTAAAGC ACTGAAAACA 
    TACAATATGC TTTGCTTTGG AATTTATCGA TTAAGGGATG CACATTTAAG TACTCCCAGC 
    CAATGCACTA AACGTTATGT TATCACAAAC CCACCGTATG AATTTGAGCT TGTGCCGACA 
    GACTTGATCT TCTGTTTGAT GCAATTTGAC CACAACGCTG GCCAGTCCCG GGCCAGTCTT 
    TCTCATTCCT CCCATTCCTC CTATTCTTCC AGTAAGAAGA GCTCATCAGT TCACTCCATC 
    CCATCAACAG CAAACCGACC GAACCGCCCC AAGACCAGGG ATTCCCGGGA AAAACAGAAT 
    GCAACAAGGA TGAATAGAAT GGGCCAAGCA GAAAAGAAAT GGTTTACAGA TGAACCGGAT 
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CTCACATGGC CAATCAGATC 
    AATCAATATA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956258.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811744.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OCa75478
    Clone ID Related Accession (Same CDS sequence) XM_014956258.1
    Accession Version XM_014956258.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTGATCTATT CCAAGATGTC CATCTACAAG AGAATGAAAC TGGCATGTTG TTTTGATTGC 
    GGACGCTCTG AGCGTGACTG CTCTTGCATG TCAGGCAGTG TACATAGTAA CATGGACACC 
    CTTGAGAGAG CCTTCCCACT TTCTTCTGTC TCTGTTAATG ATTGCTCCAC CAGTTTACGT 
    GCCTTTGAAG ATGAGCAGCC GTCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
    ATGCGAAATT CACCCAACTC CTCACCCAAA CTGATGAGGC ATGACCCCTT GTTAATTCCT 
    GGCAACGAGC AGATTGACAA TATGGATGCC AATGTGAAAA AATATGACTC TACAGGGATG 
    TTTCATTGGT GTCCAGCCAA GGACATCGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
    ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
    ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
    AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
    AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACACCATTAA GTCGGGCTGA TTTAAGGGCT 
    GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
    GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
    TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GTTTTACACC CCCTGGGATG 
    GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGCCAACC ATCCATTACA 
    ACAGGAGCCA ACATCCCTAT TATAACAGAG CTAGTAAATG ATTCAAATGT TCAGTTCTTG 
    GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TTACGCAGCC CTTTGCATGT 
    GGAACTGCAT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
    AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
    CTGATTGCTG AGGAAAATGC GTTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
    AGGGACAGGT GTCGAGTTGC TCAGTTGGCT TTGTATGATG GGCCGTTCGC AGACCTAGGG 
    GACGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
    TTTGGAATTT ATCGATTAAG GGATGCACAT TTAAGTACTC CCAGCCAATG CACTAAACGT 
    TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
    TTGATGCAAT TTGACCACAA CGCTGGCCAG TCCCGGGCCA GTCTTTCTCA TTCCTCCCAT 
    TCCTCCTATT CTTCCAGTAA GAAGAGCTCA TCAGTTCACT CCATCCCATC AACAGCAAAC 
    CGACCGAACC GCCCCAAGAC CAGGGATTCC CGGGAAAAAC AGAATGCAAC AAGGATGAAT 
    AGAATGGGCC AAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC 
    ATTCAAATCA AGCCCATGAG CACTCACATG GCCAATCAGA TCAATCAATA TAAATCGACA 
    AGCAGCTTGA TACCACCAAT CAGAGAAGTT GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811744.1
    CDS37..3801
    Translation

    Target ORF information:

    RefSeq Version XM_014956258.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956258.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTGATCTATT CCAAGATGTC CATCTACAAG AGAATGAAAC TGGCATGTTG TTTTGATTGC 
    GGACGCTCTG AGCGTGACTG CTCTTGCATG TCAGGCAGTG TACATAGTAA CATGGACACC 
    CTTGAGAGAG CCTTCCCACT TTCTTCTGTC TCTGTTAATG ATTGCTCCAC CAGTTTACGT 
    GCCTTTGAAG ATGAGCAGCC GTCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
    ATGCGAAATT CACCCAACTC CTCACCCAAA CTGATGAGGC ATGACCCCTT GTTAATTCCT 
    GGCAACGAGC AGATTGACAA TATGGATGCC AATGTGAAAA AATATGACTC TACAGGGATG 
    TTTCATTGGT GTCCAGCCAA GGACATCGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
    ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
    ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
    AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
    AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACACCATTAA GTCGGGCTGA TTTAAGGGCT 
    GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
    GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
    TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GTTTTACACC CCCTGGGATG 
    GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGCCAACC ATCCATTACA 
    ACAGGAGCCA ACATCCCTAT TATAACAGAG CTAGTAAATG ATTCAAATGT TCAGTTCTTG 
    GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TTACGCAGCC CTTTGCATGT 
    GGAACTGCAT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
    AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
    CTGATTGCTG AGGAAAATGC GTTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
    AGGGACAGGT GTCGAGTTGC TCAGTTGGCT TTGTATGATG GGCCGTTCGC AGACCTAGGG 
    GACGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
    TTTGGAATTT ATCGATTAAG GGATGCACAT TTAAGTACTC CCAGCCAATG CACTAAACGT 
    TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
    TTGATGCAAT TTGACCACAA CGCTGGCCAG TCCCGGGCCA GTCTTTCTCA TTCCTCCCAT 
    TCCTCCTATT CTTCCAGTAA GAAGAGCTCA TCAGTTCACT CCATCCCATC AACAGCAAAC 
    CGACCGAACC GCCCCAAGAC CAGGGATTCC CGGGAAAAAC AGAATGCAAC AAGGATGAAT 
    AGAATGGGCC AAGAAAAGAA ATGGTTTACA GATGAACCGG ATAATGCCTA TCCCAGAAAC 
    ATTCAAATCA AGCCCATGAG CACTCACATG GCCAATCAGA TCAATCAATA TAAATCGACA 
    AGCAGCTTGA TACCACCAAT CAGAGAAGTT GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956259.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811745.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OCa75479
    Clone ID Related Accession (Same CDS sequence) XM_014956259.1
    Accession Version XM_014956259.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
    GCAGAAAAGA AATGGTTTAC AGATGAACCG GATAATGCCT ATCCCAGAAA CATTCAAATC 
    AAGCCCATGA GCACTCACAT GGCCAATCAG ATCAATCAAT ATAAATCGAC AAGCAGCTTG 
    ATACCACCAA TCAGAGAAGT TGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811745.1
    CDS1..3756
    Translation

    Target ORF information:

    RefSeq Version XM_014956259.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956259.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
    GCAGAAAAGA AATGGTTTAC AGATGAACCG GATAATGCCT ATCCCAGAAA CATTCAAATC 
    AAGCCCATGA GCACTCACAT GGCCAATCAG ATCAATCAAT ATAAATCGAC AAGCAGCTTG 
    ATACCACCAA TCAGAGAAGT TGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956260.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811746.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OCa75480
    Clone ID Related Accession (Same CDS sequence) XM_014956260.1
    Accession Version XM_014956260.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA 
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT 
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGACACAGA ACTGTATCTT 
    ACGCAGCCCT TTGCATGTGG AACTGCATTT GCTGTCAGTG TGCTGGACTC TCTCATGAGC 
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC 
    ACACCAGAGC TGGAAGCACT GATTGCTGAG GAAAATGCGT TGAGAGGAGG TTACAGCACG 
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG 
    CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT TATGGAGATC TATTTTGTAA AGCACTGAAA 
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ATGCACATTT AAGTACTCCC 
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCGT ATGAATTTGA GCTTGTGCCG 
    ACAGACTTGA TCTTCTGTTT GATGCAATTT GACCACAACG CTGGCCAGTC CCGGGCCAGT 
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGTAAGA AGAGCTCATC AGTTCACTCC 
    ATCCCATCAA CAGCAAACCG ACCGAACCGC CCCAAGACCA GGGATTCCCG GGAAAAACAG 
    AATGCAACAA GGATGAATAG AATGGGCCAA GCAGAAAAGA AATGGTTTAC AGATGAACCG 
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACTCACAT GGCCAATCAG 
    ATCAATCAAT ATAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA 
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811746.1
    CDS1..3666
    Translation

    Target ORF information:

    RefSeq Version XM_014956260.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956260.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA 
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT 
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGACACAGA ACTGTATCTT 
    ACGCAGCCCT TTGCATGTGG AACTGCATTT GCTGTCAGTG TGCTGGACTC TCTCATGAGC 
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC 
    ACACCAGAGC TGGAAGCACT GATTGCTGAG GAAAATGCGT TGAGAGGAGG TTACAGCACG 
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG 
    CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT TATGGAGATC TATTTTGTAA AGCACTGAAA 
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ATGCACATTT AAGTACTCCC 
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCGT ATGAATTTGA GCTTGTGCCG 
    ACAGACTTGA TCTTCTGTTT GATGCAATTT GACCACAACG CTGGCCAGTC CCGGGCCAGT 
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGTAAGA AGAGCTCATC AGTTCACTCC 
    ATCCCATCAA CAGCAAACCG ACCGAACCGC CCCAAGACCA GGGATTCCCG GGAAAAACAG 
    AATGCAACAA GGATGAATAG AATGGGCCAA GCAGAAAAGA AATGGTTTAC AGATGAACCG 
    GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACTCACAT GGCCAATCAG 
    ATCAATCAAT ATAAATCGAC AAGCAGCTTG ATACCACCAA TCAGAGAAGT TGAAGATGAA 
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956261.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811747.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OCa75481
    Clone ID Related Accession (Same CDS sequence) XM_014956261.1
    Accession Version XM_014956261.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA 
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT 
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGACACAGA ACTGTATCTT 
    ACGCAGCCCT TTGCATGTGG AACTGCATTT GCTGTCAGTG TGCTGGACTC TCTCATGAGC 
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC 
    ACACCAGAGC TGGAAGCACT GATTGCTGAG GAAAATGCGT TGAGAGGAGG TTACAGCACG 
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG 
    CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT TATGGAGATC TATTTTGTAA AGCACTGAAA 
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ATGCACATTT AAGTACTCCC 
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCGT ATGAATTTGA GCTTGTGCCG 
    ACAGACTTGA TCTTCTGTTT GATGCAATTT GACCACAACG CTGGCCAGTC CCGGGCCAGT 
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGTAAGA AGAGCTCATC AGTTCACTCC 
    ATCCCATCAA CAGCAAACCG ACCGAACCGC CCCAAGACCA GGGATTCCCG GGAAAAACAG 
    AATGCAACAA GGATGAATAG AATGGGCCAA GAAAAGAAAT GGTTTACAGA TGAACCGGAT 
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CTCACATGGC CAATCAGATC 
    AATCAATATA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811747.1
    CDS1..3663
    Translation

    Target ORF information:

    RefSeq Version XM_014956261.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956261.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA 
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT 
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGACACAGA ACTGTATCTT 
    ACGCAGCCCT TTGCATGTGG AACTGCATTT GCTGTCAGTG TGCTGGACTC TCTCATGAGC 
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC 
    ACACCAGAGC TGGAAGCACT GATTGCTGAG GAAAATGCGT TGAGAGGAGG TTACAGCACG 
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG 
    CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT TATGGAGATC TATTTTGTAA AGCACTGAAA 
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ATGCACATTT AAGTACTCCC 
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCGT ATGAATTTGA GCTTGTGCCG 
    ACAGACTTGA TCTTCTGTTT GATGCAATTT GACCACAACG CTGGCCAGTC CCGGGCCAGT 
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGTAAGA AGAGCTCATC AGTTCACTCC 
    ATCCCATCAA CAGCAAACCG ACCGAACCGC CCCAAGACCA GGGATTCCCG GGAAAAACAG 
    AATGCAACAA GGATGAATAG AATGGGCCAA GAAAAGAAAT GGTTTACAGA TGAACCGGAT 
    AATGCCTATC CCAGAAACAT TCAAATCAAG CCCATGAGCA CTCACATGGC CAATCAGATC 
    AATCAATATA AATCGACAAG CAGCTTGATA CCACCAATCA GAGAAGTTGA AGATGAATGT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956263.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811749.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OCa75482
    Clone ID Related Accession (Same CDS sequence) XM_014956263.1
    Accession Version XM_014956263.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3657bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGC TAAGCCTGGC AAACTTCTGC CTTTGGTTTC AATCAGTCAG 
    GAAAAAAACA GTGGAACCCA CATTCTAATG ATAACTGAAC TGGTAAATGA TTCAAATGTT 
    CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGACACAG AACTGTATCT TACGCAGCCC 
    TTTGCATGTG GAACTGCATT TGCTGTCAGT GTGCTGGACT CTCTCATGAG CGCAACATAC 
    TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG 
    CTGGAAGCAC TGATTGCTGA GGAAAATGCG TTGAGAGGAG GTTACAGCAC GCCCCAGACA 
    CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGTTGGCTT TGTATGATGG GCCGTTCGCA 
    GACCTAGGGG ACGGAGGTTG TTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT 
    ATGCTTTGCT TTGGAATTTA TCGATTAAGG GATGCACATT TAAGTACTCC CAGCCAATGC 
    ACTAAACGTT ATGTTATCAC AAACCCACCG TATGAATTTG AGCTTGTGCC GACAGACTTG 
    ATCTTCTGTT TGATGCAATT TGACCACAAC GCTGGCCAGT CCCGGGCCAG TCTTTCTCAT 
    TCCTCCCATT CCTCCTATTC TTCCAGTAAG AAGAGCTCAT CAGTTCACTC CATCCCATCA 
    ACAGCAAACC GACCGAACCG CCCCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA 
    AGGATGAATA GAATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC 
    TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACTCACA TGGCCAATCA GATCAATCAA 
    TATAAATCGA CAAGCAGCTT GATACCACCA ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811749.1
    CDS1..3657
    Translation

    Target ORF information:

    RefSeq Version XM_014956263.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956263.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGC TAAGCCTGGC AAACTTCTGC CTTTGGTTTC AATCAGTCAG 
    GAAAAAAACA GTGGAACCCA CATTCTAATG ATAACTGAAC TGGTAAATGA TTCAAATGTT 
    CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGACACAG AACTGTATCT TACGCAGCCC 
    TTTGCATGTG GAACTGCATT TGCTGTCAGT GTGCTGGACT CTCTCATGAG CGCAACATAC 
    TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG 
    CTGGAAGCAC TGATTGCTGA GGAAAATGCG TTGAGAGGAG GTTACAGCAC GCCCCAGACA 
    CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGTTGGCTT TGTATGATGG GCCGTTCGCA 
    GACCTAGGGG ACGGAGGTTG TTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT 
    ATGCTTTGCT TTGGAATTTA TCGATTAAGG GATGCACATT TAAGTACTCC CAGCCAATGC 
    ACTAAACGTT ATGTTATCAC AAACCCACCG TATGAATTTG AGCTTGTGCC GACAGACTTG 
    ATCTTCTGTT TGATGCAATT TGACCACAAC GCTGGCCAGT CCCGGGCCAG TCTTTCTCAT 
    TCCTCCCATT CCTCCTATTC TTCCAGTAAG AAGAGCTCAT CAGTTCACTC CATCCCATCA 
    ACAGCAAACC GACCGAACCG CCCCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA 
    AGGATGAATA GAATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAACC GGATAATGCC 
    TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACTCACA TGGCCAATCA GATCAATCAA 
    TATAAATCGA CAAGCAGCTT GATACCACCA ATCAGAGAAG TTGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956264.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811750.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OCa75483
    Clone ID Related Accession (Same CDS sequence) XM_014956264.1
    Accession Version XM_014956264.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3654bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGC TAAGCCTGGC AAACTTCTGC CTTTGGTTTC AATCAGTCAG 
    GAAAAAAACA GTGGAACCCA CATTCTAATG ATAACTGAAC TGGTAAATGA TTCAAATGTT 
    CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGACACAG AACTGTATCT TACGCAGCCC 
    TTTGCATGTG GAACTGCATT TGCTGTCAGT GTGCTGGACT CTCTCATGAG CGCAACATAC 
    TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG 
    CTGGAAGCAC TGATTGCTGA GGAAAATGCG TTGAGAGGAG GTTACAGCAC GCCCCAGACA 
    CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGTTGGCTT TGTATGATGG GCCGTTCGCA 
    GACCTAGGGG ACGGAGGTTG TTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT 
    ATGCTTTGCT TTGGAATTTA TCGATTAAGG GATGCACATT TAAGTACTCC CAGCCAATGC 
    ACTAAACGTT ATGTTATCAC AAACCCACCG TATGAATTTG AGCTTGTGCC GACAGACTTG 
    ATCTTCTGTT TGATGCAATT TGACCACAAC GCTGGCCAGT CCCGGGCCAG TCTTTCTCAT 
    TCCTCCCATT CCTCCTATTC TTCCAGTAAG AAGAGCTCAT CAGTTCACTC CATCCCATCA 
    ACAGCAAACC GACCGAACCG CCCCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA 
    AGGATGAATA GAATGGGCCA AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT 
    CCCAGAAACA TTCAAATCAA GCCCATGAGC ACTCACATGG CCAATCAGAT CAATCAATAT 
    AAATCGACAA GCAGCTTGAT ACCACCAATC AGAGAAGTTG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811750.1
    CDS1..3654
    Translation

    Target ORF information:

    RefSeq Version XM_014956264.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956264.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGC TAAGCCTGGC AAACTTCTGC CTTTGGTTTC AATCAGTCAG 
    GAAAAAAACA GTGGAACCCA CATTCTAATG ATAACTGAAC TGGTAAATGA TTCAAATGTT 
    CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGACACAG AACTGTATCT TACGCAGCCC 
    TTTGCATGTG GAACTGCATT TGCTGTCAGT GTGCTGGACT CTCTCATGAG CGCAACATAC 
    TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG 
    CTGGAAGCAC TGATTGCTGA GGAAAATGCG TTGAGAGGAG GTTACAGCAC GCCCCAGACA 
    CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGTTGGCTT TGTATGATGG GCCGTTCGCA 
    GACCTAGGGG ACGGAGGTTG TTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT 
    ATGCTTTGCT TTGGAATTTA TCGATTAAGG GATGCACATT TAAGTACTCC CAGCCAATGC 
    ACTAAACGTT ATGTTATCAC AAACCCACCG TATGAATTTG AGCTTGTGCC GACAGACTTG 
    ATCTTCTGTT TGATGCAATT TGACCACAAC GCTGGCCAGT CCCGGGCCAG TCTTTCTCAT 
    TCCTCCCATT CCTCCTATTC TTCCAGTAAG AAGAGCTCAT CAGTTCACTC CATCCCATCA 
    ACAGCAAACC GACCGAACCG CCCCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA 
    AGGATGAATA GAATGGGCCA AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT 
    CCCAGAAACA TTCAAATCAA GCCCATGAGC ACTCACATGG CCAATCAGAT CAATCAATAT 
    AAATCGACAA GCAGCTTGAT ACCACCAATC AGAGAAGTTG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956265.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811751.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OCa75484
    Clone ID Related Accession (Same CDS sequence) XM_014956265.1
    Accession Version XM_014956265.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTGATCTATT TTGAAGATGA GCAGCCGTCG ACACTATCAC CCAAAAAAAA GCAGCGAAAC 
    GGAGGCATGC GAAATTCACC CAACTCCTCA CCCAAACTGA TGAGGCATGA CCCCTTGTTA 
    ATTCCTGGCA ACGAGCAGAT TGACAATATG GATGCCAATG TGAAAAAATA TGACTCTACA 
    GGGATGTTTC ATTGGTGTCC AGCCAAGGAC ATCGAAAAGG TCATTTTGAC TCGAAGTGAA 
    GCAGCGATGA CAGTTTTAAG TGGGCATGTA GTCGTTTGCA TATTTGGGGA TGTTAAATCT 
    GCTTTGATTG GATTAAGAAA TTTAGTGATG CCATTACGAG CCAGCAACTT TCACTACCAT 
    GAACTCAAGC ACATTGTGTT TGTGGGTTCA CTTGAATACC TGAGAAGGGA ATGGGAGACA 
    CTGCATAACT TCCCCAAGGT TTCAATCTTG CCTGGTACAC CATTAAGTCG GGCTGATTTA 
    AGGGCTGTCA ACATCAACCT CTGCGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
    ATTGATGATG CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
    ATGCAGTTTG ATGACAGCAT TGGGGTCTTG CAGGCTAATT CCCAAGGTTT TACACCCCCT 
    GGGATGGATA GGTCATCTCC AGACAACAGT CCAGTCCATG GCCTGTTACG CCAACCATCC 
    ATTACAACAG GAGCCAACAT CCCTATTATA ACAGAGCTAG TAAATGATTC AAATGTTCAG 
    TTCTTGGACC AAGATGATGA TGATGATCCA GACACAGAAC TGTATCTTAC GCAGCCCTTT 
    GCATGTGGAA CTGCATTTGC TGTCAGTGTG CTGGACTCTC TCATGAGCGC AACATACTTC 
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG 
    GAAGCACTGA TTGCTGAGGA AAATGCGTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT 
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC GTTCGCAGAC 
    CTAGGGGACG GAGGTTGTTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG 
    CTTTGCTTTG GAATTTATCG ATTAAGGGAT GCACATTTAA GTACTCCCAG CCAATGCACT 
    AAACGTTATG TTATCACAAA CCCACCGTAT GAATTTGAGC TTGTGCCGAC AGACTTGATC 
    TTCTGTTTGA TGCAATTTGA CCACAACGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC 
    TCCCATTCCT CCTATTCTTC CAGTAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA 
    GCAAACCGAC CGAACCGCCC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG 
    ATGAATAGAA TGGGCCAAGC AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT 
    CCCAGAAACA TTCAAATCAA GCCCATGAGC ACTCACATGG CCAATCAGAT CAATCAATAT 
    AAATCGACAA GCAGCTTGAT ACCACCAATC AGAGAAGTTG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811751.1
    CDS55..3648
    Translation

    Target ORF information:

    RefSeq Version XM_014956265.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956265.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTGATCTATT TTGAAGATGA GCAGCCGTCG ACACTATCAC CCAAAAAAAA GCAGCGAAAC 
    GGAGGCATGC GAAATTCACC CAACTCCTCA CCCAAACTGA TGAGGCATGA CCCCTTGTTA 
    ATTCCTGGCA ACGAGCAGAT TGACAATATG GATGCCAATG TGAAAAAATA TGACTCTACA 
    GGGATGTTTC ATTGGTGTCC AGCCAAGGAC ATCGAAAAGG TCATTTTGAC TCGAAGTGAA 
    GCAGCGATGA CAGTTTTAAG TGGGCATGTA GTCGTTTGCA TATTTGGGGA TGTTAAATCT 
    GCTTTGATTG GATTAAGAAA TTTAGTGATG CCATTACGAG CCAGCAACTT TCACTACCAT 
    GAACTCAAGC ACATTGTGTT TGTGGGTTCA CTTGAATACC TGAGAAGGGA ATGGGAGACA 
    CTGCATAACT TCCCCAAGGT TTCAATCTTG CCTGGTACAC CATTAAGTCG GGCTGATTTA 
    AGGGCTGTCA ACATCAACCT CTGCGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
    ATTGATGATG CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
    ATGCAGTTTG ATGACAGCAT TGGGGTCTTG CAGGCTAATT CCCAAGGTTT TACACCCCCT 
    GGGATGGATA GGTCATCTCC AGACAACAGT CCAGTCCATG GCCTGTTACG CCAACCATCC 
    ATTACAACAG GAGCCAACAT CCCTATTATA ACAGAGCTAG TAAATGATTC AAATGTTCAG 
    TTCTTGGACC AAGATGATGA TGATGATCCA GACACAGAAC TGTATCTTAC GCAGCCCTTT 
    GCATGTGGAA CTGCATTTGC TGTCAGTGTG CTGGACTCTC TCATGAGCGC AACATACTTC 
    AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG 
    GAAGCACTGA TTGCTGAGGA AAATGCGTTG AGAGGAGGTT ACAGCACGCC CCAGACACTT 
    GCAAACAGGG ACAGGTGTCG AGTTGCTCAG TTGGCTTTGT ATGATGGGCC GTTCGCAGAC 
    CTAGGGGACG GAGGTTGTTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG 
    CTTTGCTTTG GAATTTATCG ATTAAGGGAT GCACATTTAA GTACTCCCAG CCAATGCACT 
    AAACGTTATG TTATCACAAA CCCACCGTAT GAATTTGAGC TTGTGCCGAC AGACTTGATC 
    TTCTGTTTGA TGCAATTTGA CCACAACGCT GGCCAGTCCC GGGCCAGTCT TTCTCATTCC 
    TCCCATTCCT CCTATTCTTC CAGTAAGAAG AGCTCATCAG TTCACTCCAT CCCATCAACA 
    GCAAACCGAC CGAACCGCCC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG 
    ATGAATAGAA TGGGCCAAGC AGAAAAGAAA TGGTTTACAG ATGAACCGGA TAATGCCTAT 
    CCCAGAAACA TTCAAATCAA GCCCATGAGC ACTCACATGG CCAATCAGAT CAATCAATAT 
    AAATCGACAA GCAGCTTGAT ACCACCAATC AGAGAAGTTG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956266.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811752.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID OCa75485
    Clone ID Related Accession (Same CDS sequence) XM_014956266.1
    Accession Version XM_014956266.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTTGAAGATG AGCAGCCGTC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
    AACGAGCAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
    CATTGGTGTC CAGCCAAGGA CATCGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TTACACCCCC TGGGATGGAT 
    AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GCCAACCATC CATTACAACA 
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC 
    CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTTA CGCAGCCCTT TGCATGTGGA 
    ACTGCATTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
    ATTGCTGAGG AAAATGCGTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CGTTCGCAGA CCTAGGGGAC 
    GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
    GGAATTTATC GATTAAGGGA TGCACATTTA AGTACTCCCA GCCAATGCAC TAAACGTTAT 
    GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
    ATGCAATTTG ACCACAACGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC 
    TCCTATTCTT CCAGTAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA 
    CCGAACCGCC CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
    ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT 
    CAAATCAAGC CCATGAGCAC TCACATGGCC AATCAGATCA ATCAATATAA ATCGACAAGC 
    AGCTTGATAC CACCAATCAG AGAAGTTGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811752.1
    CDS55..3636
    Translation

    Target ORF information:

    RefSeq Version XM_014956266.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956266.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTTGAAGATG AGCAGCCGTC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
    AACGAGCAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
    CATTGGTGTC CAGCCAAGGA CATCGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TTACACCCCC TGGGATGGAT 
    AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GCCAACCATC CATTACAACA 
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC 
    CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTTA CGCAGCCCTT TGCATGTGGA 
    ACTGCATTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
    ATTGCTGAGG AAAATGCGTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CGTTCGCAGA CCTAGGGGAC 
    GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
    GGAATTTATC GATTAAGGGA TGCACATTTA AGTACTCCCA GCCAATGCAC TAAACGTTAT 
    GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
    ATGCAATTTG ACCACAACGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC 
    TCCTATTCTT CCAGTAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA 
    CCGAACCGCC CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
    ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAACCGGATA ATGCCTATCC CAGAAACATT 
    CAAATCAAGC CCATGAGCAC TCACATGGCC AATCAGATCA ATCAATATAA ATCGACAAGC 
    AGCTTGATAC CACCAATCAG AGAAGTTGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956267.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811753.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID OCa75486
    Clone ID Related Accession (Same CDS sequence) XM_014956267.1
    Accession Version XM_014956267.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA 
    GATGATGATG ATGATCCAGA CACAGAACTG TATCTTACGC AGCCCTTTGC ATGTGGAACT 
    GCATTTGCTG TCAGTGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC 
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT 
    GCTGAGGAAA ATGCGTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC 
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCGT TCGCAGACCT AGGGGACGGA 
    GGTTGTTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA 
    ATTTATCGAT TAAGGGATGC ACATTTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT 
    ATCACAAACC CACCGTATGA ATTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG 
    CAATTTGACC ACAACGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC 
    TATTCTTCCA GTAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG 
    AACCGCCCCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG 
    GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA 
    ATCAAGCCCA TGAGCACTCA CATGGCCAAT CAGATCAATC AATATAAATC GACAAGCAGC 
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811753.1
    CDS55..3633
    Translation

    Target ORF information:

    RefSeq Version XM_014956267.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956267.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGTA AATGATTCAA ATGTTCAGTT CTTGGACCAA 
    GATGATGATG ATGATCCAGA CACAGAACTG TATCTTACGC AGCCCTTTGC ATGTGGAACT 
    GCATTTGCTG TCAGTGTGCT GGACTCTCTC ATGAGCGCAA CATACTTCAA TGACAATATC 
    CTCACACTGA TACGGACATT GGTAACAGGA GGAGCCACAC CAGAGCTGGA AGCACTGATT 
    GCTGAGGAAA ATGCGTTGAG AGGAGGTTAC AGCACGCCCC AGACACTTGC AAACAGGGAC 
    AGGTGTCGAG TTGCTCAGTT GGCTTTGTAT GATGGGCCGT TCGCAGACCT AGGGGACGGA 
    GGTTGTTATG GAGATCTATT TTGTAAAGCA CTGAAAACAT ACAATATGCT TTGCTTTGGA 
    ATTTATCGAT TAAGGGATGC ACATTTAAGT ACTCCCAGCC AATGCACTAA ACGTTATGTT 
    ATCACAAACC CACCGTATGA ATTTGAGCTT GTGCCGACAG ACTTGATCTT CTGTTTGATG 
    CAATTTGACC ACAACGCTGG CCAGTCCCGG GCCAGTCTTT CTCATTCCTC CCATTCCTCC 
    TATTCTTCCA GTAAGAAGAG CTCATCAGTT CACTCCATCC CATCAACAGC AAACCGACCG 
    AACCGCCCCA AGACCAGGGA TTCCCGGGAA AAACAGAATG CAACAAGGAT GAATAGAATG 
    GGCCAAGAAA AGAAATGGTT TACAGATGAA CCGGATAATG CCTATCCCAG AAACATTCAA 
    ATCAAGCCCA TGAGCACTCA CATGGCCAAT CAGATCAATC AATATAAATC GACAAGCAGC 
    TTGATACCAC CAATCAGAGA AGTTGAAGAT GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956268.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811754.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X12, mRNA.
    Price & Availability↑
    CloneID OCa75487
    Clone ID Related Accession (Same CDS sequence) XM_014956268.1
    Accession Version XM_014956268.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT 
    GATGATCCAG ACACAGAACT GTATCTTACG CAGCCCTTTG CATGTGGAAC TGCATTTGCT 
    GTCAGTGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG 
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCTGAGGAA 
    AATGCGTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA 
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCG TTCGCAGACC TAGGGGACGG AGGTTGTTAT 
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA 
    TTAAGGGATG CACATTTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC 
    CCACCGTATG AATTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTTGAC 
    CACAACGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC 
    AGTAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCCCC 
    AAGACCAGGG ATTCCCGGGA AAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCAAGCA 
    GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG 
    CCCATGAGCA CTCACATGGC CAATCAGATC AATCAATATA AATCGACAAG CAGCTTGATA 
    CCACCAATCA GAGAAGTTGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811754.1
    CDS1..3573
    Translation

    Target ORF information:

    RefSeq Version XM_014956268.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT 
    GATGATCCAG ACACAGAACT GTATCTTACG CAGCCCTTTG CATGTGGAAC TGCATTTGCT 
    GTCAGTGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG 
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCTGAGGAA 
    AATGCGTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA 
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCG TTCGCAGACC TAGGGGACGG AGGTTGTTAT 
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA 
    TTAAGGGATG CACATTTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC 
    CCACCGTATG AATTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTTGAC 
    CACAACGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC 
    AGTAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCCCC 
    AAGACCAGGG ATTCCCGGGA AAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCAAGCA 
    GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG 
    CCCATGAGCA CTCACATGGC CAATCAGATC AATCAATATA AATCGACAAG CAGCTTGATA 
    CCACCAATCA GAGAAGTTGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956269.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811755.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X13, mRNA.
    Price & Availability↑
    CloneID OCa75488
    Clone ID Related Accession (Same CDS sequence) XM_014956269.1
    Accession Version XM_014956269.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTTGAAGATG AGCAGCCGTC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
    AACGAGCAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
    CATTGGTGTC CAGCCAAGGA CATCGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TTACACCCCC TGGGATGGAT 
    AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GCCAACCATC CATTACAACA 
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT 
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
    CTTACGCAGC CCTTTGCATG TGGAACTGCA TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
    GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CGTTGAGAGG AGGTTACAGC 
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT 
    GGGCCGTTCG CAGACCTAGG GGACGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TTTAAGTACT 
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ACGCTGGCCA GTCCCGGGCC 
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGTA AGAAGAGCTC ATCAGTTCAC 
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCCCCAAGA CCAGGGATTC CCGGGAAAAA 
    CAGAACAGAA AAGAAATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811755.1
    CDS37..3546
    Translation

    Target ORF information:

    RefSeq Version XM_014956269.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTTGAAGATG AGCAGCCGTC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
    AACGAGCAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
    CATTGGTGTC CAGCCAAGGA CATCGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TTACACCCCC TGGGATGGAT 
    AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GCCAACCATC CATTACAACA 
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
    TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTAA TGATAACTGA ACTGGTAAAT 
    GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
    CTTACGCAGC CCTTTGCATG TGGAACTGCA TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
    AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
    GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CGTTGAGAGG AGGTTACAGC 
    ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGTTGGC TTTGTATGAT 
    GGGCCGTTCG CAGACCTAGG GGACGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
    AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TTTAAGTACT 
    CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
    CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ACGCTGGCCA GTCCCGGGCC 
    AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGTA AGAAGAGCTC ATCAGTTCAC 
    TCCATCCCAT CAACAGCAAA CCGACCGAAC CGCCCCAAGA CCAGGGATTC CCGGGAAAAA 
    CAGAACAGAA AAGAAATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956270.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811756.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X14, mRNA.
    Price & Availability↑
    CloneID OCa75489
    Clone ID Related Accession (Same CDS sequence) XM_014956270.1
    Accession Version XM_014956270.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA 
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT 
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGACACAGA ACTGTATCTT 
    ACGCAGCCCT TTGCATGTGG AACTGCATTT GCTGTCAGTG TGCTGGACTC TCTCATGAGC 
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC 
    ACACCAGAGC TGGAAGCACT GATTGCTGAG GAAAATGCGT TGAGAGGAGG TTACAGCACG 
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG 
    CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT TATGGAGATC TATTTTGTAA AGCACTGAAA 
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ATGCACATTT AAGTACTCCC 
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCGT ATGAATTTGA GCTTGTGCCG 
    ACAGACTTGA TCTTCTGTTT GATGCAATTT GACCACAACG CTGGCCAGTC CCGGGCCAGT 
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGTAAGA AGAGCTCATC AGTTCACTCC 
    ATCCCATCAA CAGCAAACCG ACCGAACCGC CCCAAGACCA GGGATTCCCG GGAAAAACAG 
    AAGTATGTGC AGGAAGATAG ACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811756.1
    CDS1..3507
    Translation

    Target ORF information:

    RefSeq Version XM_014956270.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956270.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA 
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT 
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGACACAGA ACTGTATCTT 
    ACGCAGCCCT TTGCATGTGG AACTGCATTT GCTGTCAGTG TGCTGGACTC TCTCATGAGC 
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC 
    ACACCAGAGC TGGAAGCACT GATTGCTGAG GAAAATGCGT TGAGAGGAGG TTACAGCACG 
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG 
    CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT TATGGAGATC TATTTTGTAA AGCACTGAAA 
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ATGCACATTT AAGTACTCCC 
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCGT ATGAATTTGA GCTTGTGCCG 
    ACAGACTTGA TCTTCTGTTT GATGCAATTT GACCACAACG CTGGCCAGTC CCGGGCCAGT 
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGTAAGA AGAGCTCATC AGTTCACTCC 
    ATCCCATCAA CAGCAAACCG ACCGAACCGC CCCAAGACCA GGGATTCCCG GGAAAAACAG 
    AAGTATGTGC AGGAAGATAG ACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956271.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811757.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X15, mRNA.
    Price & Availability↑
    CloneID OCa75490
    Clone ID Related Accession (Same CDS sequence) XM_014956271.1
    Accession Version XM_014956271.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA 
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT 
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGACACAGA ACTGTATCTT 
    ACGCAGCCCT TTGCATGTGG AACTGCATTT GCTGTCAGTG TGCTGGACTC TCTCATGAGC 
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC 
    ACACCAGAGC TGGAAGCACT GATTGCTGAG GAAAATGCGT TGAGAGGAGG TTACAGCACG 
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG 
    CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT TATGGAGATC TATTTTGTAA AGCACTGAAA 
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ATGCACATTT AAGTACTCCC 
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCGT ATGAATTTGA GCTTGTGCCG 
    ACAGACTTGA TCTTCTGTTT GATGCAATTT GACCACAACG CTGGCCAGTC CCGGGCCAGT 
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGTAAGA AGAGCTCATC AGTTCACTCC 
    ATCCCATCAA CAGCAAACCG ACCGAACCGC CCCAAGACCA GGGATTCCCG GGAAAAACAG 
    AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811757.1
    CDS1..3504
    Translation

    Target ORF information:

    RefSeq Version XM_014956271.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956271.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTTT 
    GAAGATGAGC AGCCGTCGAC ACTATCACCC AAAAAAAAGC AGCGAAACGG AGGCATGCGA 
    AATTCACCCA ACTCCTCACC CAAACTGATG AGGCATGACC CCTTGTTAAT TCCTGGCAAC 
    GAGCAGATTG ACAATATGGA TGCCAATGTG AAAAAATATG ACTCTACAGG GATGTTTCAT 
    TGGTGTCCAG CCAAGGACAT CGAAAAGGTC ATTTTGACTC GAAGTGAAGC AGCGATGACA 
    GTTTTAAGTG GGCATGTAGT CGTTTGCATA TTTGGGGATG TTAAATCTGC TTTGATTGGA 
    TTAAGAAATT TAGTGATGCC ATTACGAGCC AGCAACTTTC ACTACCATGA ACTCAAGCAC 
    ATTGTGTTTG TGGGTTCACT TGAATACCTG AGAAGGGAAT GGGAGACACT GCATAACTTC 
    CCCAAGGTTT CAATCTTGCC TGGTACACCA TTAAGTCGGG CTGATTTAAG GGCTGTCAAC 
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAATAATAT TGATGATGCT 
    TCGCTGCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA TCAAATCTAT GCAGTTTGAT 
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTTA CACCCCCTGG GATGGATAGG 
    TCATCTCCAG ACAACAGTCC AGTCCATGGC CTGTTACGCC AACCATCCAT TACAACAGGA 
    GCCAACATCC CTATTATAAC AGAGCTAGCT AAGCCTGGCA AACTTCTGCC TTTGGTTTCA 
    ATCAGTCAGG AAAAAAACAG TGGAACCCAC ATTCTAATGA TAACTGAACT GGTAAATGAT 
    TCAAATGTTC AGTTCTTGGA CCAAGATGAT GATGATGATC CAGACACAGA ACTGTATCTT 
    ACGCAGCCCT TTGCATGTGG AACTGCATTT GCTGTCAGTG TGCTGGACTC TCTCATGAGC 
    GCAACATACT TCAATGACAA TATCCTCACA CTGATACGGA CATTGGTAAC AGGAGGAGCC 
    ACACCAGAGC TGGAAGCACT GATTGCTGAG GAAAATGCGT TGAGAGGAGG TTACAGCACG 
    CCCCAGACAC TTGCAAACAG GGACAGGTGT CGAGTTGCTC AGTTGGCTTT GTATGATGGG 
    CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT TATGGAGATC TATTTTGTAA AGCACTGAAA 
    ACATACAATA TGCTTTGCTT TGGAATTTAT CGATTAAGGG ATGCACATTT AAGTACTCCC 
    AGCCAATGCA CTAAACGTTA TGTTATCACA AACCCACCGT ATGAATTTGA GCTTGTGCCG 
    ACAGACTTGA TCTTCTGTTT GATGCAATTT GACCACAACG CTGGCCAGTC CCGGGCCAGT 
    CTTTCTCATT CCTCCCATTC CTCCTATTCT TCCAGTAAGA AGAGCTCATC AGTTCACTCC 
    ATCCCATCAA CAGCAAACCG ACCGAACCGC CCCAAGACCA GGGATTCCCG GGAAAAACAG 
    AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956272.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811758.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X16, mRNA.
    Price & Availability↑
    CloneID OCa75491
    Clone ID Related Accession (Same CDS sequence) XM_014956272.1
    Accession Version XM_014956272.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTTGAAGATG AGCAGCCGTC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
    AACGAGCAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
    CATTGGTGTC CAGCCAAGGA CATCGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TTACACCCCC TGGGATGGAT 
    AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GCCAACCATC CATTACAACA 
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC 
    CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTTA CGCAGCCCTT TGCATGTGGA 
    ACTGCATTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
    ATTGCTGAGG AAAATGCGTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CGTTCGCAGA CCTAGGGGAC 
    GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
    GGAATTTATC GATTAAGGGA TGCACATTTA AGTACTCCCA GCCAATGCAC TAAACGTTAT 
    GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
    ATGCAATTTG ACCACAACGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC 
    TCCTATTCTT CCAGTAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA 
    CCGAACCGCC CCAAGACCAG GGATTCCCGG GAAAAACAGA ACAGAAAAGA AATGGTTTAC 
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811758.1
    CDS37..3462
    Translation

    Target ORF information:

    RefSeq Version XM_014956272.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956272.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTTGAAGATG AGCAGCCGTC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
    AACGAGCAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
    CATTGGTGTC CAGCCAAGGA CATCGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TTACACCCCC TGGGATGGAT 
    AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GCCAACCATC CATTACAACA 
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC 
    CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTTA CGCAGCCCTT TGCATGTGGA 
    ACTGCATTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
    ATTGCTGAGG AAAATGCGTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CGTTCGCAGA CCTAGGGGAC 
    GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
    GGAATTTATC GATTAAGGGA TGCACATTTA AGTACTCCCA GCCAATGCAC TAAACGTTAT 
    GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
    ATGCAATTTG ACCACAACGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC 
    TCCTATTCTT CCAGTAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA 
    CCGAACCGCC CCAAGACCAG GGATTCCCGG GAAAAACAGA ACAGAAAAGA AATGGTTTAC 
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956274.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811760.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X17, mRNA.
    Price & Availability↑
    CloneID OCa75492
    Clone ID Related Accession (Same CDS sequence) XM_014956274.1
    Accession Version XM_014956274.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTTGAAGATG AGCAGCCGTC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
    AACGAGCAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
    CATTGGTGTC CAGCCAAGGA CATCGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TTACACCCCC TGGGATGGAT 
    AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GCCAACCATC CATTACAACA 
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC 
    CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTTA CGCAGCCCTT TGCATGTGGA 
    ACTGCATTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
    ATTGCTGAGG AAAATGCGTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CGTTCGCAGA CCTAGGGGAC 
    GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
    GGAATTTATC GATTAAGGGA TGCACATTTA AGTACTCCCA GCCAATGCAC TAAACGTTAT 
    GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
    ATGCAATTTG ACCACAACGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC 
    TCCTATTCTT CCAGTAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA 
    CCGAACCGCC CCAAGACCAG GGATTCCCGG GAAAAACAGA AAAAAGAAAT GGTTTACAGA 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811760.1
    CDS55..3477
    Translation

    Target ORF information:

    RefSeq Version XM_014956274.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956274.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGAAGCC GAAAGCGTAT ATTAATCAAT CCTGGAAACC ATGTCAAGAT TCAAGAAGGT 
    ACTTTAGGAT TCTTCATTGC AAGTGATGCC AAAGAAGTAA AAAGGGCATT TTTTTACTGC 
    AAGGCCTGTC ATGATGACAT CACGGATCCC AAAAGAATAA AAAAATGTGG CTGCAAACGG 
    CTTGAAGATG AGCAGCCGTC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
    CGAAATTCAC CCAACTCCTC ACCCAAACTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
    AACGAGCAGA TTGACAATAT GGATGCCAAT GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
    CATTGGTGTC CAGCCAAGGA CATCGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
    ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
    GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
    CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
    TTCCCCAAGG TTTCAATCTT GCCTGGTACA CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
    GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TTACACCCCC TGGGATGGAT 
    AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GCCAACCATC CATTACAACA 
    GGAGCCAACA TCCCTATTAT AACAGAGCTA GTAAATGATT CAAATGTTCA GTTCTTGGAC 
    CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTTA CGCAGCCCTT TGCATGTGGA 
    ACTGCATTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
    ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
    ATTGCTGAGG AAAATGCGTT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
    GACAGGTGTC GAGTTGCTCA GTTGGCTTTG TATGATGGGC CGTTCGCAGA CCTAGGGGAC 
    GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
    GGAATTTATC GATTAAGGGA TGCACATTTA AGTACTCCCA GCCAATGCAC TAAACGTTAT 
    GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
    ATGCAATTTG ACCACAACGC TGGCCAGTCC CGGGCCAGTC TTTCTCATTC CTCCCATTCC 
    TCCTATTCTT CCAGTAAGAA GAGCTCATCA GTTCACTCCA TCCCATCAAC AGCAAACCGA 
    CCGAACCGCC CCAAGACCAG GGATTCCCGG GAAAAACAGA AAAAAGAAAT GGTTTACAGA 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956275.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811761.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X18, mRNA.
    Price & Availability↑
    CloneID OCa75493
    Clone ID Related Accession (Same CDS sequence) XM_014956275.1
    Accession Version XM_014956275.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT 
    GATGATCCAG ACACAGAACT GTATCTTACG CAGCCCTTTG CATGTGGAAC TGCATTTGCT 
    GTCAGTGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG 
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCTGAGGAA 
    AATGCGTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA 
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCG TTCGCAGACC TAGGGGACGG AGGTTGTTAT 
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA 
    TTAAGGGATG CACATTTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC 
    CCACCGTATG AATTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTTGAC 
    CACAACGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC 
    AGTAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCCCC 
    AAGACCAGGG ATTCCCGGGA AAAACAGAAG TATGTGCAGG AAGATAGACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811761.1
    CDS55..3468
    Translation

    Target ORF information:

    RefSeq Version XM_014956275.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT 
    GATGATCCAG ACACAGAACT GTATCTTACG CAGCCCTTTG CATGTGGAAC TGCATTTGCT 
    GTCAGTGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG 
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCTGAGGAA 
    AATGCGTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA 
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCG TTCGCAGACC TAGGGGACGG AGGTTGTTAT 
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA 
    TTAAGGGATG CACATTTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC 
    CCACCGTATG AATTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTTGAC 
    CACAACGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC 
    AGTAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCCCC 
    AAGACCAGGG ATTCCCGGGA AAAACAGAAG TATGTGCAGG AAGATAGACT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956276.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811762.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X19, mRNA.
    Price & Availability↑
    CloneID OCa75494
    Clone ID Related Accession (Same CDS sequence) XM_014956276.1
    Accession Version XM_014956276.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT 
    GATGATCCAG ACACAGAACT GTATCTTACG CAGCCCTTTG CATGTGGAAC TGCATTTGCT 
    GTCAGTGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG 
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCTGAGGAA 
    AATGCGTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA 
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCG TTCGCAGACC TAGGGGACGG AGGTTGTTAT 
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA 
    TTAAGGGATG CACATTTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC 
    CCACCGTATG AATTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTTGAC 
    CACAACGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC 
    AGTAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCCCC 
    AAGACCAGGG ATTCCCGGGA AAAACAGAAA AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811762.1
    CDS55..3465
    Translation

    Target ORF information:

    RefSeq Version XM_014956276.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT 
    GATGATCCAG ACACAGAACT GTATCTTACG CAGCCCTTTG CATGTGGAAC TGCATTTGCT 
    GTCAGTGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG 
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCTGAGGAA 
    AATGCGTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA 
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCG TTCGCAGACC TAGGGGACGG AGGTTGTTAT 
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA 
    TTAAGGGATG CACATTTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC 
    CCACCGTATG AATTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTTGAC 
    CACAACGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC 
    AGTAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCCCC 
    AAGACCAGGG ATTCCCGGGA AAAACAGAAA AAAGAAATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956277.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811763.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X20, mRNA.
    Price & Availability↑
    CloneID OCa75495
    Clone ID Related Accession (Same CDS sequence) XM_014956277.1
    Accession Version XM_014956277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT 
    GATGATCCAG ACACAGAACT GTATCTTACG CAGCCCTTTG CATGTGGAAC TGCATTTGCT 
    GTCAGTGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG 
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCTGAGGAA 
    AATGCGTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA 
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCG TTCGCAGACC TAGGGGACGG AGGTTGTTAT 
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA 
    TTAAGGGATG CACATTTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC 
    CCACCGTATG AATTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTTGAC 
    CACAACGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC 
    AGTAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCCCC 
    AAGACCAGGG ATTCCCGGGA AAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCAAGAA 
    AAGAAATGGT TTACAGATGA ACCGGATAAT GCCTATCCCA GAAACATTCA AATCAAGCCC 
    ATGAGCACTC ACATGGCCAA TCAGATCAAT CAATATAAAT CGACAAGCAG CTTGATACCA 
    CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811763.1
    CDS56..3625
    Translation

    Target ORF information:

    RefSeq Version XM_014956277.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT TGAAGATGAG 
    CAGCCGTCGA CACTATCACC CAAAAAAAAG CAGCGAAACG GAGGCATGCG AAATTCACCC 
    AACTCCTCAC CCAAACTGAT GAGGCATGAC CCCTTGTTAA TTCCTGGCAA CGAGCAGATT 
    GACAATATGG ATGCCAATGT GAAAAAATAT GACTCTACAG GGATGTTTCA TTGGTGTCCA 
    GCCAAGGACA TCGAAAAGGT CATTTTGACT CGAAGTGAAG CAGCGATGAC AGTTTTAAGT 
    GGGCATGTAG TCGTTTGCAT ATTTGGGGAT GTTAAATCTG CTTTGATTGG ATTAAGAAAT 
    TTAGTGATGC CATTACGAGC CAGCAACTTT CACTACCATG AACTCAAGCA CATTGTGTTT 
    GTGGGTTCAC TTGAATACCT GAGAAGGGAA TGGGAGACAC TGCATAACTT CCCCAAGGTT 
    TCAATCTTGC CTGGTACACC ATTAAGTCGG GCTGATTTAA GGGCTGTCAA CATCAACCTC 
    TGCGACATGT GCGTTATCCT GTCAGCCAAT CAGAATAATA TTGATGATGC TTCGCTGCAG 
    GACAAGGAAT GCATCTTGGC GTCACTCAAC ATCAAATCTA TGCAGTTTGA TGACAGCATT 
    GGGGTCTTGC AGGCTAATTC CCAAGGTTTT ACACCCCCTG GGATGGATAG GTCATCTCCA 
    GACAACAGTC CAGTCCATGG CCTGTTACGC CAACCATCCA TTACAACAGG AGCCAACATC 
    CCTATTATAA CAGAGCTAGT AAATGATTCA AATGTTCAGT TCTTGGACCA AGATGATGAT 
    GATGATCCAG ACACAGAACT GTATCTTACG CAGCCCTTTG CATGTGGAAC TGCATTTGCT 
    GTCAGTGTGC TGGACTCTCT CATGAGCGCA ACATACTTCA ATGACAATAT CCTCACACTG 
    ATACGGACAT TGGTAACAGG AGGAGCCACA CCAGAGCTGG AAGCACTGAT TGCTGAGGAA 
    AATGCGTTGA GAGGAGGTTA CAGCACGCCC CAGACACTTG CAAACAGGGA CAGGTGTCGA 
    GTTGCTCAGT TGGCTTTGTA TGATGGGCCG TTCGCAGACC TAGGGGACGG AGGTTGTTAT 
    GGAGATCTAT TTTGTAAAGC ACTGAAAACA TACAATATGC TTTGCTTTGG AATTTATCGA 
    TTAAGGGATG CACATTTAAG TACTCCCAGC CAATGCACTA AACGTTATGT TATCACAAAC 
    CCACCGTATG AATTTGAGCT TGTGCCGACA GACTTGATCT TCTGTTTGAT GCAATTTGAC 
    CACAACGCTG GCCAGTCCCG GGCCAGTCTT TCTCATTCCT CCCATTCCTC CTATTCTTCC 
    AGTAAGAAGA GCTCATCAGT TCACTCCATC CCATCAACAG CAAACCGACC GAACCGCCCC 
    AAGACCAGGG ATTCCCGGGA AAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCAAGAA 
    AAGAAATGGT TTACAGATGA ACCGGATAAT GCCTATCCCA GAAACATTCA AATCAAGCCC 
    ATGAGCACTC ACATGGCCAA TCAGATCAAT CAATATAAAT CGACAAGCAG CTTGATACCA 
    CCAATCAGAG AAGTTGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956278.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811764.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X21, mRNA.
    Price & Availability↑
    CloneID OCa75496
    Clone ID Related Accession (Same CDS sequence) XM_014956278.1
    Accession Version XM_014956278.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
    GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG 
    CCCATGAGCA CTCACATGGC CAATCAGATC AATCAATATA AATCGACAAG CAGCTTGATA 
    CCACCAATCA GAGAAGTTGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811764.1
    CDS37..3789
    Translation

    Target ORF information:

    RefSeq Version XM_014956278.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956278.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
    GAAAAGAAAT GGTTTACAGA TGAACCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG 
    CCCATGAGCA CTCACATGGC CAATCAGATC AATCAATATA AATCGACAAG CAGCTTGATA 
    CCACCAATCA GAGAAGTTGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956279.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811765.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X22, mRNA.
    Price & Availability↑
    CloneID OCa75497
    Clone ID Related Accession (Same CDS sequence) XM_014956279.1
    Accession Version XM_014956279.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AAGTATGTGC AGGAAGATAG ACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811765.1
    CDS37..3633
    Translation

    Target ORF information:

    RefSeq Version XM_014956279.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956279.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AAGTATGTGC AGGAAGATAG ACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956280.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811766.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X23, mRNA.
    Price & Availability↑
    CloneID OCa75498
    Clone ID Related Accession (Same CDS sequence) XM_014956280.1
    Accession Version XM_014956280.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811766.1
    CDS37..3630
    Translation

    Target ORF information:

    RefSeq Version XM_014956280.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956280.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AAAAAAGAAA TGGTTTACAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    KCNMA1 ( XM_014956281.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014811767.1 Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X24, mRNA.
    Price & Availability↑
    CloneID OCa75499
    Clone ID Related Accession (Same CDS sequence) XM_014956281.1
    Accession Version XM_014956281.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product calcium-activated potassium channel subunit alpha-1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091042.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014811767.1
    CDS37..3633
    Translation

    Target ORF information:

    RefSeq Version XM_014956281.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax potassium channel, calcium activated large conductance subfamily M alpha, member 1 (KCNMA1), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014956281.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAACA ATATCAACGC CAACAATCTC AACACAGACT CGTCCTCCTC CCCCGTCAAT 
    GTGCCCAAGA TGGATGCGCT CATCATCCCG GTGACCATGG AGGTGCCCTG CGATAGCCGC 
    GGACAGCGCA TGTGGTGGGC TTTCCTCGCC TCATCCATGG TCACTTTCTT TGGGGGACTG 
    TTTATCATCC TGCTGTGGAG GACCCTCAAG TACCTGTGGA CTGTCTGCTG CCACTGCGGG 
    GTCAAAAACA AGGAAGCCCA GAAGATCAAT GGTGGTGGAG ATACACAGGC AGATGGGGCC 
    TGCAAACCAA CAGATGAAAA AGAGGAGAAT GTGGCAGCAG AAGTGGGGTG GATGACCTCT 
    GTGAAGGATT GGGCAGGAGT CATGATATCT GCCCAGACAT TAACTGGCAG AGTACTTGTT 
    GTCTTAGTCT TCGCTCTTAG TATTGGTGCT CTTGTAATAT ACTTCATAGA TTCATCAAAC 
    CCAATAGAAT CCTGCCAGAA TTTCTACAAA GATTTCACGT TACAGATTGA CATGGCTTTC 
    AATGTGTTCT TCCTACTCTA CTTCGGCTTG CGTTTCATAG CAGCCAATGA CAAGCTATGG 
    TTTTGGCTAG AAGTTAACTC AGTAGTAGAT TTCTTCACGG TCCCTCCAGT GTTTGTGTCA 
    GTATATTTAA ACAGAAGCTG GCTTGGTTTA AGATTTTTAA GAGCTCTTAG ACTGATACAA 
    TTTTCAGAAA TTTTGCAGTT TCTGAATATC CTTAAAACAA GTAACTCCAT CAAGCTAGTG 
    AATCTGTGCT CTATATTTAT CAGCACGTGG CTGACAGCAG CTGGGTTCAT ACATTTGGTG 
    GAGAACTCAG GGGATCCATG GGAAAATTTC CAAAATAATC AACCGCTAAC ATATTGGGAA 
    TGTGTTTATT TACTGATGGT TACAATGTCC ACTGTTGGCT ATGGAGATGT TTATGCAAAA 
    ACAACCCTTG GTCGCCTTTT CATGGTCTTC TTCATCCTTG GGGGATTGGC CATGTTTGCC 
    AGCTACGTCC CTGAAATCAT AGAGTTAATA GGAAACCGCA AGAAATATGG TGGTTCCTAT 
    AGTGCGGTTA GTGGAAGAAA GCACATAGTT GTCTGTGGTC ACATAACACT TGAAAGTGTG 
    TCCAACTTCC TGAAGGACTT CCTGCACAAG GATAGGGATG ATGTCAACGT AGAAATCGTC 
    TTTCTGCATA ATATCTCCCC TAACCTTGAG CTGGAAGCTC TTTTTAAACG ACACTTCACT 
    CAGGTGGAAT TTTATCAGGG TTCAGTCCTT AATCCCCATG ACCTGGCGAG AGTGAAGATA 
    GAGTCAGCAG ATGCGTGCCT AATCCTTGCC AATAAATACT GCGCTGACCC AGATGCTGAA 
    GATGCCTCCA ATATTATGAG AGTTATTTCA ATAAAGAATT ATCATCCGAA GATAAGAATT 
    ATCACTCAGA TGTTGCAGTA TCACAATAAG GCTCATCTGC TAAATATCCC AAGCTGGAAC 
    TGGAAAGAAG GGGATGATGC AATCTGTCTT GCTGAGCTCA AGCTGGGTTT CATAGCCCAG 
    AGCTGCCTGG CACCGGGCCT TTCAACAATG TTAGCCAACC TCTTTTCTAT GAGGTCATTC 
    ATAAAGATTG AGGAGGATAC GTGGCAGAAA TACTACCTGG AAGGAGTTGC AAATGAAATG 
    TATACAGAAT ATCTGTCTAG TGCCTTTGTG GGTTTGTCCT TCCCTGCAGT TTGTGAACTG 
    GTGTTTGCGA AACTAAAGCT CTTAATGATA GCCATAGAAT ACAAGTCGGA AAAAAGAGAA 
    AGCAGTATAT TAATCAATCC TGGAAACCAT GTCAAGATTC AAGAAGGTAC TTTAGGATTC 
    TTCATTGCAA GTGATGCCAA AGAAGTAAAA AGGGCATTTT TTTACTGCAA GGCCTGTCAT 
    GATGACATCA CGGATCCCAA AAGAATAAAA AAATGTGGCT GCAAACGGCT GATCTATTCC 
    AAGATGTCCA TCTACAAGAG AATGAAACTG GCATGTTGTT TTGATTGCGG ACGCTCTGAG 
    CGTGACTGCT CTTGCATGTC AGGCAGTGTA CATAGTAACA TGGACACCCT TGAGAGAGCC 
    TTCCCACTTT CTTCTGTCTC TGTTAATGAT TGCTCCACCA GTTTACGTGC CTTTGAAGAT 
    GAGCAGCCGT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GCGAAATTCA 
    CCCAACTCCT CACCCAAACT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAACGAGCAG 
    ATTGACAATA TGGATGCCAA TGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
    CCAGCCAAGG ACATCGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
    AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
    AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
    TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
    GTTTCAATCT TGCCTGGTAC ACCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
    CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
    CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
    ATTGGGGTCT TGCAGGCTAA TTCCCAAGGT TTTACACCCC CTGGGATGGA TAGGTCATCT 
    CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGCCAACCAT CCATTACAAC AGGAGCCAAC 
    ATCCCTATTA TAACAGAGCT AGTAAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
    GATGATGATC CAGACACAGA ACTGTATCTT ACGCAGCCCT TTGCATGTGG AACTGCATTT 
    GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
    CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
    GAAAATGCGT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
    CGAGTTGCTC AGTTGGCTTT GTATGATGGG CCGTTCGCAG ACCTAGGGGA CGGAGGTTGT 
    TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
    CGATTAAGGG ATGCACATTT AAGTACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
    AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
    GACCACAACG CTGGCCAGTC CCGGGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
    TCCAGTAAGA AGAGCTCATC AGTTCACTCC ATCCCATCAA CAGCAAACCG ACCGAACCGC 
    CCCAAGACCA GGGATTCCCG GGAAAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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1-877-436-7274

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