RIMS2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	RIMS2
Entrez Gene ID	106902210
Full Name	regulating synaptic membrane exocytosis 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014965515.1	XP_014821001.1	regulating synaptic membrane exocytosis protein 2 isoform X1
XM_014965519.1	XP_014821005.1	regulating synaptic membrane exocytosis protein 2 isoform X5
XM_014965516.1	XP_014821002.1	regulating synaptic membrane exocytosis protein 2 isoform X2
XM_014965517.1	XP_014821003.1	regulating synaptic membrane exocytosis protein 2 isoform X3
XM_014965520.1	XP_014821006.1	regulating synaptic membrane exocytosis protein 2 isoform X6
XM_014965518.1	XP_014821004.1	regulating synaptic membrane exocytosis protein 2 isoform X4
XM_014965521.1	XP_014821007.1	regulating synaptic membrane exocytosis protein 2 isoform X7

Pathways from BioSystems
Gene Ontology

The following RIMS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIMS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa81972	XM_014965519.1 Latest version!	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCa81973	XM_014965520.1 Latest version!	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OCa81969	XM_014965516.1 Latest version!	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa81970	XM_014965517.1 Latest version!	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa81971	XM_014965518.1 Latest version!	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa81968	XM_014965515.1 Latest version!	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCa81974	XM_014965521.1 Latest version!	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIMS2 ( XM_014965519.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821005.1 Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCa81972
Clone ID Related Accession (Same CDS sequence)	XM_014965519.1
Accession Version	XM_014965519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	regulating synaptic membrane exocytosis protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAG AAGAGAGGAA TCGCCAAATG AAAATGAGCA AGTACAAACA GGTAGCAGGA 
TCAGATTCCA GGCTGGAACA AGATTATCAT TCTAAGTATC GGTCAGGACG GGATCCTCAG 
CGAGGCTCAG ACAACGTTTC CAATAAGTCT TCAGACAGCG ACGTCAGTGA CGTCTCCGCC 
GTGTCACGGA CAAGCAGCGC TTCACGTTTC AGCAGCACAA GCTACATGTC TGTCCAGTCG 
GAGCGGCCAA GGGGAAACAA AAAAATAAGT GTCTTTACAT CCAAAATGCA AAGCAGACAG 
ATGGGCGCCT CAGGAAAGAA CATGACTAAG AGCACCAGCA TCGGCGGGGA CATGTACACG 
CTGGAGAAGA ACGACGGCAG CCAGTCAGAC ACCGCAGTGG GCACTGTTGG TGGTGGTGGC 
AAAAAGAGAC GCTCCAGCAT CGGTGCAAAG ATGGTCGCCA TTGTGGGTCT CTCGCGAAAA 
AGCCGTAGCA CGTCACAACT CAGCCAAACT GAAGCAGGGG GCAAGAAGCT GCGGAGCACC 
GTCCAGAGGA GCACTGAGAC AGGGCTGGCC GTGGAGATGA GGAACTGGAT GACCCGTCAG 
GCCAGCCGGG AGTCAACGGA TGGGAGCATG AACAGCTACA GCTCTGAGGG AAATTTGATT 
TTCCCTGGTG TGAGGTTGGC TGCAGACAGC CAGTTCAGTG ATTTTCTGGA TGGACTTGGT 
CCTGCCCAGC TTGTAGGGCG ACAGACTTTG GCGACACCAT CGATGGGAGA TATTCAGGTG 
GGAATGATGG ACAAGAAAGG ACAATTGGAA GTAGAAATAA TCCGAGCTCG TGGCCTTGTT 
GTAAAACCAG GTTCAAAGAC ACTGCCAGCA CCATATGTAA AGGTGTATCT ATTAGAAAAT 
GGAGTCTGCA TAGCCAAAAA GAAAACAAAG GTAGCAAGAA AAACGCTGGA ACCGCTTTAT 
CAGCAACTTC TGTCATTTGA AGAAAGTCCT CAAGGCAAAG TTTTACAGAT AATCGTTTGG 
GGAGACTATG GACGTATGGA TCACAAGTCC TTTATGGGAG TGGCACAGAT ACTTTTAGAT 
GAACTGGACC TGTCCAACAT GGTGATTGGG TGGTTCAAAC TCTTCCCTCC TTCTTCCCTA 
GTAGACCCAA CTTTAGCTCC TCTCACTAGA AGAGCTTCCC AATCATCTCT TGAAAGTTCA 
ACAGGACCTT CGTATGCTCG CTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014821005.1
CDS	93..1319
Translation	MDVEERNRQMKMSKYKQVAGSDSRLEQDYHSKYRSGRDPQRGSDNVSNKSSDSDVSDVSAVSRTSSASRFSSTSYMSVQSERPRGNKKISVFTSKMQSRQMGASGKNMTKSTSIGGDMYTLEKNDGSQSDTAVGTVGGGGKKRRSSIGAKMVAIVGLSRKSRSTSQLSQTEAGGKKLRSTVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLAADSQFSDFLDGLGPAQLVGRQTLATPSMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLENGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELDLSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYARS

Target ORF information:

RefSeq Version	XM_014965519.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014965519.1

ORF Insert Sequence:

ATGGATGTAG AAGAGAGGAA TCGCCAAATG AAAATGAGCA AGTACAAACA GGTAGCAGGA 
TCAGATTCCA GGCTGGAACA AGATTATCAT TCTAAGTATC GGTCAGGACG GGATCCTCAG 
CGAGGCTCAG ACAACGTTTC CAATAAGTCT TCAGACAGCG ACGTCAGTGA CGTCTCCGCC 
GTGTCACGGA CAAGCAGCGC TTCACGTTTC AGCAGCACAA GCTACATGTC TGTCCAGTCG 
GAGCGGCCAA GGGGAAACAA AAAAATAAGT GTCTTTACAT CCAAAATGCA AAGCAGACAG 
ATGGGCGCCT CAGGAAAGAA CATGACTAAG AGCACCAGCA TCGGCGGGGA CATGTACACG 
CTGGAGAAGA ACGACGGCAG CCAGTCAGAC ACCGCAGTGG GCACTGTTGG TGGTGGTGGC 
AAAAAGAGAC GCTCCAGCAT CGGTGCAAAG ATGGTCGCCA TTGTGGGTCT CTCGCGAAAA 
AGCCGTAGCA CGTCACAACT CAGCCAAACT GAAGCAGGGG GCAAGAAGCT GCGGAGCACC 
GTCCAGAGGA GCACTGAGAC AGGGCTGGCC GTGGAGATGA GGAACTGGAT GACCCGTCAG 
GCCAGCCGGG AGTCAACGGA TGGGAGCATG AACAGCTACA GCTCTGAGGG AAATTTGATT 
TTCCCTGGTG TGAGGTTGGC TGCAGACAGC CAGTTCAGTG ATTTTCTGGA TGGACTTGGT 
CCTGCCCAGC TTGTAGGGCG ACAGACTTTG GCGACACCAT CGATGGGAGA TATTCAGGTG 
GGAATGATGG ACAAGAAAGG ACAATTGGAA GTAGAAATAA TCCGAGCTCG TGGCCTTGTT 
GTAAAACCAG GTTCAAAGAC ACTGCCAGCA CCATATGTAA AGGTGTATCT ATTAGAAAAT 
GGAGTCTGCA TAGCCAAAAA GAAAACAAAG GTAGCAAGAA AAACGCTGGA ACCGCTTTAT 
CAGCAACTTC TGTCATTTGA AGAAAGTCCT CAAGGCAAAG TTTTACAGAT AATCGTTTGG 
GGAGACTATG GACGTATGGA TCACAAGTCC TTTATGGGAG TGGCACAGAT ACTTTTAGAT 
GAACTGGACC TGTCCAACAT GGTGATTGGG TGGTTCAAAC TCTTCCCTCC TTCTTCCCTA 
GTAGACCCAA CTTTAGCTCC TCTCACTAGA AGAGCTTCCC AATCATCTCT TGAAAGTTCA 
ACAGGACCTT CGTATGCTCG CTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIMS2 ( XM_014965520.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821006.1 Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCa81973
Clone ID Related Accession (Same CDS sequence)	XM_014965520.1
Accession Version	XM_014965520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	regulating synaptic membrane exocytosis protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGTC AGACGGCGGG CGGCGGGCGC AGCATGCAGC GGTCCCAGAG CCGGAGCAGC 
CTCTCCGCCT CCTTCGAGGC GCTGGCCGTC TACTTCCCCT GCATGAACTC CCTGGAGGAG 
GAGGACGGAG AAGCAGGGGG CAAGAAGCTG CGGAGCACCG TCCAGAGGAG CACTGAGACA 
GGGCTGGCCG TGGAGATGAG GAACTGGATG ACCCGTCAGG CCAGCCGGGA GTCAACGGAT 
GGGAGCATGA ACAGCTACAG CTCTGAGGGA AATTTGATTT TCCCTGGTGT GAGGTTGGCT 
GCAGACAGCC AGTTCAGTGA TTTTCTGGAT GGACTTGGTC CTGCCCAGCT TGTAGGGCGA 
CAGACTTTGG CGACACCATC GATGGGAGAT ATTCAGGTGG GAATGATGGA CAAGAAAGGA 
CAATTGGAAG TAGAAATAAT CCGAGCTCGT GGCCTTGTTG TAAAACCAGG TTCAAAGACA 
CTGCCAGCAC CATATGTAAA GGTGTATCTA TTAGAAAATG GAGTCTGCAT AGCCAAAAAG 
AAAACAAAGG TAGCAAGAAA AACGCTGGAA CCGCTTTATC AGCAACTTCT GTCATTTGAA 
GAAAGTCCTC AAGGCAAAGT TTTACAGATA ATCGTTTGGG GAGACTATGG ACGTATGGAT 
CACAAGTCCT TTATGGGAGT GGCACAGATA CTTTTAGATG AACTGGACCT GTCCAACATG 
GTGATTGGGT GGTTCAAACT CTTCCCTCCT TCTTCCCTAG TAGACCCAAC TTTAGCTCCT 
CTCACTAGAA GAGCTTCCCA ATCATCTCTT GAAAGTTCAA CAGGACCTTC GTATGCTCGC 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014821006.1
CDS	21..866
Translation	MGRQTAGGGRSMQRSQSRSSLSASFEALAVYFPCMNSLEEEDGEAGGKKLRSTVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLAADSQFSDFLDGLGPAQLVGRQTLATPSMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLENGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELDLSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYARS

Target ORF information:

RefSeq Version	XM_014965520.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014965520.1

ORF Insert Sequence:

ATGGGCCGTC AGACGGCGGG CGGCGGGCGC AGCATGCAGC GGTCCCAGAG CCGGAGCAGC 
CTCTCCGCCT CCTTCGAGGC GCTGGCCGTC TACTTCCCCT GCATGAACTC CCTGGAGGAG 
GAGGACGGAG AAGCAGGGGG CAAGAAGCTG CGGAGCACCG TCCAGAGGAG CACTGAGACA 
GGGCTGGCCG TGGAGATGAG GAACTGGATG ACCCGTCAGG CCAGCCGGGA GTCAACGGAT 
GGGAGCATGA ACAGCTACAG CTCTGAGGGA AATTTGATTT TCCCTGGTGT GAGGTTGGCT 
GCAGACAGCC AGTTCAGTGA TTTTCTGGAT GGACTTGGTC CTGCCCAGCT TGTAGGGCGA 
CAGACTTTGG CGACACCATC GATGGGAGAT ATTCAGGTGG GAATGATGGA CAAGAAAGGA 
CAATTGGAAG TAGAAATAAT CCGAGCTCGT GGCCTTGTTG TAAAACCAGG TTCAAAGACA 
CTGCCAGCAC CATATGTAAA GGTGTATCTA TTAGAAAATG GAGTCTGCAT AGCCAAAAAG 
AAAACAAAGG TAGCAAGAAA AACGCTGGAA CCGCTTTATC AGCAACTTCT GTCATTTGAA 
GAAAGTCCTC AAGGCAAAGT TTTACAGATA ATCGTTTGGG GAGACTATGG ACGTATGGAT 
CACAAGTCCT TTATGGGAGT GGCACAGATA CTTTTAGATG AACTGGACCT GTCCAACATG 
GTGATTGGGT GGTTCAAACT CTTCCCTCCT TCTTCCCTAG TAGACCCAAC TTTAGCTCCT 
CTCACTAGAA GAGCTTCCCA ATCATCTCTT GAAAGTTCAA CAGGACCTTC GTATGCTCGC 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIMS2 ( XM_014965516.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821002.1 Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa81969
Clone ID Related Accession (Same CDS sequence)	XM_014965516.1
Accession Version	XM_014965516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	regulating synaptic membrane exocytosis protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATTTG AGACACTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
TTCTCATCCC CACCAAATAT TTTACAAAAT GATCTTTTTG GACAAACGTT GAACAACACT 
AGGAAAAGAA GCCCATCAAT ATCAAGAGAA CAGAACAGAA GATACGACCA AAGGGACGAG 
AGAGACGAAT ACTCACAGTA TGCTACATCA GACAGTGCGA TGCCCAGGTC ACCATCAGAT 
TATTCCGATA GGCGGTCGCA GCGTGGGCCT CAGTTATATG AAGAGCCTGA ACTGGGTGAT 
TATAGAGATT CCAACAGAAG GAGTCGCAGG CGTTCCAAGG AGTATCCGGT AGAAGAGGAA 
GATGTACAAA ACAGGGAAGA ATATGAAAGG CAGCGGAGGG AAGAGGAATA CCAGGCACGG 
TACCGAAGCG ATCCAAATTT GGCTCGTTAT CCAGTCAAGC CGCAACCGTA TGAGGAACAA 
ATGCGTATCC ATGCTGAAGT GTCCCGGGCA CGTCATGAAC GGAGACATAG TGATGTCTCA 
TTGGCAAATA CTGAGTTGGA AGATTCTCGG ATGTCTATGC TGAGGATGGA ACGACCATCA 
AGACAAAGAT CAGTGTCTGA GCGTAGGGCT GCCATGGAAA ATCAGCGCTC ATACTCTATG 
GAAAGAACTC GAGAGGCTCA GGGACCAAGT CCCAATCGCC AGAGGACCAC AAATCATAGT 
CCTCCTACAC CTAGGAGGAG TCCAATTCCT CTGGAAAGAC CAGACATGAG GCGCTCTGAT 
TCATTAAGGA AACAACACCA TTTGGATCCC AATTCTGCTG TACGGAAAAC AAAACGTGAA 
AAGATGGAGA CCATGTTACG GAATGATTCG CTCAGCTCAG ACCAGTCTGA ATCTGTCAGA 
CCTCCACCAC CAAAGCCCCA TAAAACGAAA AAGGGAGGTA AAATGCGCCA GGTTTCATTA 
AGTAGTTCAG AAGAAGAATT GGCGTCCACT CCAGAGTATA CAAGTTGTGA TGATGTAGAG 
ATTGAAAGTG AAAGTGTTAG TGAAAAAGGG GACAGTCAAA GGGGAAAAAG AAAAACTAGT 
GAGCAGGCAG TTTTGTCGGA TTCTAACACC TTATCCCAGA GGCAAAAGAA AATGGTGTGC 
TTTGGTGGCC ACTCTTTGGA AGAGGACTTG GAATGGTCTG AGCCTCAGAT AAAAGACTCT 
GGGGTAGATA CGTGTAGTAG CACAACTCTA AACGAGGAAC ATAGCCATAG CGAGAAGCAT 
CCAGTAACTT GGCAACCATC CAAAGATGGA GATCGTCTCA TTGGTCGGAT TTTGTTAAAT 
AAACGCCTAA AAGATGGAAG TGTGCCTAGA GACTCAGGAG CAATGCTTGG ATTGAAGGTA 
GTAGGAGGAA AGATGACTGA ATCAGGTCGA CTCTGCGCAT TTATCACCAA AGTTAAAAAA 
GGAAGCTTAG CCGATACAGT GGGGCATCTC AGACCAGGTG ATGAAGTATT AGAATGGAAT 
GGAAGGCTAC TACAAGGAGC CACGTTTGAG GAAGTGTATA ACATCATTTT AGAATCCAAA 
CCTGAGCCAC AAGTGGAGCT TGTTGTTTCA AGACCAATAG GGGACATTCC CAGAATACCT 
GACAGCACAC ATGCACAGCT GGAGTCCAGC TCTAGTTCAT TTGAATCTCA AAAAATGGAC 
CGACCTTCTA TTTCAGTGAC TTCTCCTATG AGTCCTGGCA TGTTAAGGGA TGTTCCACAG 
TTCTTGTCTG GACAACTTTC AAGCCAAAGC CTTAGTAGAA GAACAACGCC TTTTGTTCCT 
AGGGTTCAGA TAAAACTGTG GTATGACAAG GTTGGTCATC AGTTAATAGT GACGATATTG 
GGAGCAAAGG ATCTTCCTTC AAGGGAAGAT GGGAGGCCAA GGAATCCTTA TGTTAAAATT 
TACTTTCTTC CAGACAGAAG TGATAAAAAT AAAAGAAGAA CAAAAACAGT AAAGAAAACA 
TTGGAACCTA AGTGGAATCA GACATTCATT TATTCTCCAG TTCATCGGAG AGAGTTTAGA 
GAACGAATGC TAGAAATTAC TCTTTGGGAC CAAGCACGAG TTCGAGAGGA AGAAAGTGAA 
TTTTTAGGAG AGATTCTTAT TGAGTTAGAG ACAGCTTTAC TAGATGACGA ACCTCACTGG 
TACAAACTTC AAACGCATGA TGTCTCTTCA TTGCCACTTC CCCATCCCTC ACCATATTTG 
CCACGCAGAC AGCTCCATGG TGAGAGTCCC ACACGGAGGT TACAAAATAA AGGCTTATAT 
TCATATAATT CAGGGTCCAA AAGAATCAGC GACAGTGAAG TCTCTGATTA TGACTGTGAA 
GATGGAATTG GTGTTGTTTC AGATTATCGA CATAATGGGA GAGATCTTCA AAGTTCAACG 
CTATCGGTGC CAGAACAAGT TATGTCATCT AATCATTGCT CTCGATCAGG GTCCCCTCAC 
CGTGGAGATA GTATAGGACG GACTAGATCG TGGTCTCCCA GTGTCCCTCC CCCACAAAGT 
CGGAATGTGG ATCAGGGACC ACGAGGGACT CGATCTACTC CTGCTCATTA CAATACCCTG 
AACCGAATGG ATAGACACCG TGTCATAGAT GACCACTACT CCCCGGACAG AGAGAGGAAT 
TGTGAAGCAG CAGATAGACA GCCATATCAA AGATCCAGAT CAACAGAGCA ACGTCCTATG 
CTAGAGCGGA CCAACTCCCG CTCCCGATCC ACAGAGCGTC CTGACTCAAA CATCATCAGG 
TCGATGCCTT CATTAATGAC TGGAAGATCT GCCCCTCCTT CACCTGCCTT ACCGAGGTCA 
CATCCTCGAA CTGGGTCTGT TCAGACAAGT CCATCAAGTA CTCCAGTAGT AGGGCGACGG 
GGCAGGCAGT TGCCGCAGCT GCCACCAAAG GGGACATTGG AGAGAAAAGA AGCGGAATCA 
TCAAGGAGAA GAAACTCAGA GGAATGCCCT GAGCCATTTA CAACCTTTGA AGAAAAGAAA 
GCAGATCCAG AGAATGGGGA TACAGGTGCA ATGGATGTAG AAGAGAGGAA TCGCCAAATG 
AAAATGAGCA AGTACAAACA GGTAGCAGGA TCAGATTCCA GGCTGGAACA AGATTATCAT 
TCTAAGTATC GGTCAGGACG GGATCCTCAG CGAGGCTCAG ACAACGTTTC CAATAAGTCT 
TCAGACAGCG ACGTCAGTGA CGTCTCCGCC GTGTCACGGA CAAGCAGCGC TTCACGTTTC 
AGCAGCACAA GCTACATGTC TGTCCAGTCG GAGCGGCCAA GGGGAAACAA AAAAATAAGT 
GTCTTTACAT CCAAAATGCA AAGCAGACAG ATGGGCGCCT CAGGAAAGAA CATGACTAAG 
AGCACCAGCA TCGGCGGGGA CATGTACACG CTGGAGAAGA ACGACGGCAG CCAGTCAGAC 
ACCGCAGTGG GCACTGTTGG TGGTGGTGGC AAAAAGAGAC GCTCCAGCAT CGGTGCAAAG 
ATGGTCGCCA TTGTGGGTCT CTCGCGAAAA AGCCGTAGCA CGTCACAACT CAGCCAAACT 
GAAGCAGGGG GCAAGAAGCT GCGGAGCACC GTCCAGAGGA GCACTGAGAC AGGGCTGGCC 
GTGGAGATGA GGAACTGGAT GACCCGTCAG GCCAGCCGGG AGTCAACGGA TGGGAGCATG 
AACAGCTACA GCTCTGAGGG AAATTTGATT TTCCCTGGTG TGAGGTTGGC TGCAGACAGC 
CAGTTCAGTG ATTTTCTGGA TGGACTTGGT CCTGCCCAGC TTGTAGGGCG ACAGACTTTG 
GCGACACCAT CGATGGGAGA TATTCAGGTG GGAATGATGG ACAAGAAAGG ACAATTGGAA 
GTAGAAATAA TCCGAGCTCG TGGCCTTGTT GTAAAACCAG GTTCAAAGAC ACTGCCAGCA 
CCATATGTAA AGGTGTATCT ATTAGAAAAT GGAGTCTGCA TAGCCAAAAA GAAAACAAAG 
GTAGCAAGAA AAACGCTGGA ACCGCTTTAT CAGCAACTTC TGTCATTTGA AGAAAGTCCT 
CAAGGCAAAG TTTTACAGAT AATCGTTTGG GGAGACTATG GACGTATGGA TCACAAGTCC 
TTTATGGGAG TGGCACAGAT ACTTTTAGAT GAACTGGACC TGTCCAACAT GGTGATTGGG 
TGGTTCAAAC TCTTCCCTCC TTCTTCCCTA GTAGACCCAA CTTTAGCTCC TCTCACTAGA 
AGAGCTTCCC AATCATCTCT TGAAAGTTCA ACAGGACCTT CGTATGCTCG CTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014821002.1
CDS	386..4642
Translation	MQFETLRQVCNSVLSHFHGVFSSPPNILQNDLFGQTLNNTRKRSPSISREQNRRYDQRDERDEYSQYATSDSAMPRSPSDYSDRRSQRGPQLYEEPELGDYRDSNRRSRRRSKEYPVEEEDVQNREEYERQRREEEYQARYRSDPNLARYPVKPQPYEEQMRIHAEVSRARHERRHSDVSLANTELEDSRMSMLRMERPSRQRSVSERRAAMENQRSYSMERTREAQGPSPNRQRTTNHSPPTPRRSPIPLERPDMRRSDSLRKQHHLDPNSAVRKTKREKMETMLRNDSLSSDQSESVRPPPPKPHKTKKGGKMRQVSLSSSEEELASTPEYTSCDDVEIESESVSEKGDSQRGKRKTSEQAVLSDSNTLSQRQKKMVCFGGHSLEEDLEWSEPQIKDSGVDTCSSTTLNEEHSHSEKHPVTWQPSKDGDRLIGRILLNKRLKDGSVPRDSGAMLGLKVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGATFEEVYNIILESKPEPQVELVVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRPSISVTSPMSPGMLRDVPQFLSGQLSSQSLSRRTTPFVPRVQIKLWYDKVGHQLIVTILGAKDLPSREDGRPRNPYVKIYFLPDRSDKNKRRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARVREEESEFLGEILIELETALLDDEPHWYKLQTHDVSSLPLPHPSPYLPRRQLHGESPTRRLQNKGLYSYNSGSKRISDSEVSDYDCEDGIGVVSDYRHNGRDLQSSTLSVPEQVMSSNHCSRSGSPHRGDSIGRTRSWSPSVPPPQSRNVDQGPRGTRSTPAHYNTLNRMDRHRVIDDHYSPDRERNCEAADRQPYQRSRSTEQRPMLERTNSRSRSTERPDSNIIRSMPSLMTGRSAPPSPALPRSHPRTGSVQTSPSSTPVVGRRGRQLPQLPPKGTLERKEAESSRRRNSEECPEPFTTFEEKKADPENGDTGAMDVEERNRQMKMSKYKQVAGSDSRLEQDYHSKYRSGRDPQRGSDNVSNKSSDSDVSDVSAVSRTSSASRFSSTSYMSVQSERPRGNKKISVFTSKMQSRQMGASGKNMTKSTSIGGDMYTLEKNDGSQSDTAVGTVGGGGKKRRSSIGAKMVAIVGLSRKSRSTSQLSQTEAGGKKLRSTVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLAADSQFSDFLDGLGPAQLVGRQTLATPSMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLENGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELDLSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYARS

Target ORF information:

RefSeq Version	XM_014965516.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014965516.1

ORF Insert Sequence:

ATGCAATTTG AGACACTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
TTCTCATCCC CACCAAATAT TTTACAAAAT GATCTTTTTG GACAAACGTT GAACAACACT 
AGGAAAAGAA GCCCATCAAT ATCAAGAGAA CAGAACAGAA GATACGACCA AAGGGACGAG 
AGAGACGAAT ACTCACAGTA TGCTACATCA GACAGTGCGA TGCCCAGGTC ACCATCAGAT 
TATTCCGATA GGCGGTCGCA GCGTGGGCCT CAGTTATATG AAGAGCCTGA ACTGGGTGAT 
TATAGAGATT CCAACAGAAG GAGTCGCAGG CGTTCCAAGG AGTATCCGGT AGAAGAGGAA 
GATGTACAAA ACAGGGAAGA ATATGAAAGG CAGCGGAGGG AAGAGGAATA CCAGGCACGG 
TACCGAAGCG ATCCAAATTT GGCTCGTTAT CCAGTCAAGC CGCAACCGTA TGAGGAACAA 
ATGCGTATCC ATGCTGAAGT GTCCCGGGCA CGTCATGAAC GGAGACATAG TGATGTCTCA 
TTGGCAAATA CTGAGTTGGA AGATTCTCGG ATGTCTATGC TGAGGATGGA ACGACCATCA 
AGACAAAGAT CAGTGTCTGA GCGTAGGGCT GCCATGGAAA ATCAGCGCTC ATACTCTATG 
GAAAGAACTC GAGAGGCTCA GGGACCAAGT CCCAATCGCC AGAGGACCAC AAATCATAGT 
CCTCCTACAC CTAGGAGGAG TCCAATTCCT CTGGAAAGAC CAGACATGAG GCGCTCTGAT 
TCATTAAGGA AACAACACCA TTTGGATCCC AATTCTGCTG TACGGAAAAC AAAACGTGAA 
AAGATGGAGA CCATGTTACG GAATGATTCG CTCAGCTCAG ACCAGTCTGA ATCTGTCAGA 
CCTCCACCAC CAAAGCCCCA TAAAACGAAA AAGGGAGGTA AAATGCGCCA GGTTTCATTA 
AGTAGTTCAG AAGAAGAATT GGCGTCCACT CCAGAGTATA CAAGTTGTGA TGATGTAGAG 
ATTGAAAGTG AAAGTGTTAG TGAAAAAGGG GACAGTCAAA GGGGAAAAAG AAAAACTAGT 
GAGCAGGCAG TTTTGTCGGA TTCTAACACC TTATCCCAGA GGCAAAAGAA AATGGTGTGC 
TTTGGTGGCC ACTCTTTGGA AGAGGACTTG GAATGGTCTG AGCCTCAGAT AAAAGACTCT 
GGGGTAGATA CGTGTAGTAG CACAACTCTA AACGAGGAAC ATAGCCATAG CGAGAAGCAT 
CCAGTAACTT GGCAACCATC CAAAGATGGA GATCGTCTCA TTGGTCGGAT TTTGTTAAAT 
AAACGCCTAA AAGATGGAAG TGTGCCTAGA GACTCAGGAG CAATGCTTGG ATTGAAGGTA 
GTAGGAGGAA AGATGACTGA ATCAGGTCGA CTCTGCGCAT TTATCACCAA AGTTAAAAAA 
GGAAGCTTAG CCGATACAGT GGGGCATCTC AGACCAGGTG ATGAAGTATT AGAATGGAAT 
GGAAGGCTAC TACAAGGAGC CACGTTTGAG GAAGTGTATA ACATCATTTT AGAATCCAAA 
CCTGAGCCAC AAGTGGAGCT TGTTGTTTCA AGACCAATAG GGGACATTCC CAGAATACCT 
GACAGCACAC ATGCACAGCT GGAGTCCAGC TCTAGTTCAT TTGAATCTCA AAAAATGGAC 
CGACCTTCTA TTTCAGTGAC TTCTCCTATG AGTCCTGGCA TGTTAAGGGA TGTTCCACAG 
TTCTTGTCTG GACAACTTTC AAGCCAAAGC CTTAGTAGAA GAACAACGCC TTTTGTTCCT 
AGGGTTCAGA TAAAACTGTG GTATGACAAG GTTGGTCATC AGTTAATAGT GACGATATTG 
GGAGCAAAGG ATCTTCCTTC AAGGGAAGAT GGGAGGCCAA GGAATCCTTA TGTTAAAATT 
TACTTTCTTC CAGACAGAAG TGATAAAAAT AAAAGAAGAA CAAAAACAGT AAAGAAAACA 
TTGGAACCTA AGTGGAATCA GACATTCATT TATTCTCCAG TTCATCGGAG AGAGTTTAGA 
GAACGAATGC TAGAAATTAC TCTTTGGGAC CAAGCACGAG TTCGAGAGGA AGAAAGTGAA 
TTTTTAGGAG AGATTCTTAT TGAGTTAGAG ACAGCTTTAC TAGATGACGA ACCTCACTGG 
TACAAACTTC AAACGCATGA TGTCTCTTCA TTGCCACTTC CCCATCCCTC ACCATATTTG 
CCACGCAGAC AGCTCCATGG TGAGAGTCCC ACACGGAGGT TACAAAATAA AGGCTTATAT 
TCATATAATT CAGGGTCCAA AAGAATCAGC GACAGTGAAG TCTCTGATTA TGACTGTGAA 
GATGGAATTG GTGTTGTTTC AGATTATCGA CATAATGGGA GAGATCTTCA AAGTTCAACG 
CTATCGGTGC CAGAACAAGT TATGTCATCT AATCATTGCT CTCGATCAGG GTCCCCTCAC 
CGTGGAGATA GTATAGGACG GACTAGATCG TGGTCTCCCA GTGTCCCTCC CCCACAAAGT 
CGGAATGTGG ATCAGGGACC ACGAGGGACT CGATCTACTC CTGCTCATTA CAATACCCTG 
AACCGAATGG ATAGACACCG TGTCATAGAT GACCACTACT CCCCGGACAG AGAGAGGAAT 
TGTGAAGCAG CAGATAGACA GCCATATCAA AGATCCAGAT CAACAGAGCA ACGTCCTATG 
CTAGAGCGGA CCAACTCCCG CTCCCGATCC ACAGAGCGTC CTGACTCAAA CATCATCAGG 
TCGATGCCTT CATTAATGAC TGGAAGATCT GCCCCTCCTT CACCTGCCTT ACCGAGGTCA 
CATCCTCGAA CTGGGTCTGT TCAGACAAGT CCATCAAGTA CTCCAGTAGT AGGGCGACGG 
GGCAGGCAGT TGCCGCAGCT GCCACCAAAG GGGACATTGG AGAGAAAAGA AGCGGAATCA 
TCAAGGAGAA GAAACTCAGA GGAATGCCCT GAGCCATTTA CAACCTTTGA AGAAAAGAAA 
GCAGATCCAG AGAATGGGGA TACAGGTGCA ATGGATGTAG AAGAGAGGAA TCGCCAAATG 
AAAATGAGCA AGTACAAACA GGTAGCAGGA TCAGATTCCA GGCTGGAACA AGATTATCAT 
TCTAAGTATC GGTCAGGACG GGATCCTCAG CGAGGCTCAG ACAACGTTTC CAATAAGTCT 
TCAGACAGCG ACGTCAGTGA CGTCTCCGCC GTGTCACGGA CAAGCAGCGC TTCACGTTTC 
AGCAGCACAA GCTACATGTC TGTCCAGTCG GAGCGGCCAA GGGGAAACAA AAAAATAAGT 
GTCTTTACAT CCAAAATGCA AAGCAGACAG ATGGGCGCCT CAGGAAAGAA CATGACTAAG 
AGCACCAGCA TCGGCGGGGA CATGTACACG CTGGAGAAGA ACGACGGCAG CCAGTCAGAC 
ACCGCAGTGG GCACTGTTGG TGGTGGTGGC AAAAAGAGAC GCTCCAGCAT CGGTGCAAAG 
ATGGTCGCCA TTGTGGGTCT CTCGCGAAAA AGCCGTAGCA CGTCACAACT CAGCCAAACT 
GAAGCAGGGG GCAAGAAGCT GCGGAGCACC GTCCAGAGGA GCACTGAGAC AGGGCTGGCC 
GTGGAGATGA GGAACTGGAT GACCCGTCAG GCCAGCCGGG AGTCAACGGA TGGGAGCATG 
AACAGCTACA GCTCTGAGGG AAATTTGATT TTCCCTGGTG TGAGGTTGGC TGCAGACAGC 
CAGTTCAGTG ATTTTCTGGA TGGACTTGGT CCTGCCCAGC TTGTAGGGCG ACAGACTTTG 
GCGACACCAT CGATGGGAGA TATTCAGGTG GGAATGATGG ACAAGAAAGG ACAATTGGAA 
GTAGAAATAA TCCGAGCTCG TGGCCTTGTT GTAAAACCAG GTTCAAAGAC ACTGCCAGCA 
CCATATGTAA AGGTGTATCT ATTAGAAAAT GGAGTCTGCA TAGCCAAAAA GAAAACAAAG 
GTAGCAAGAA AAACGCTGGA ACCGCTTTAT CAGCAACTTC TGTCATTTGA AGAAAGTCCT 
CAAGGCAAAG TTTTACAGAT AATCGTTTGG GGAGACTATG GACGTATGGA TCACAAGTCC 
TTTATGGGAG TGGCACAGAT ACTTTTAGAT GAACTGGACC TGTCCAACAT GGTGATTGGG 
TGGTTCAAAC TCTTCCCTCC TTCTTCCCTA GTAGACCCAA CTTTAGCTCC TCTCACTAGA 
AGAGCTTCCC AATCATCTCT TGAAAGTTCA ACAGGACCTT CGTATGCTCG CTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIMS2 ( XM_014965517.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821003.1 Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X3, mRNA.

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CloneID	OCa81970
Clone ID Related Accession (Same CDS sequence)	XM_014965517.1
Accession Version	XM_014965517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	regulating synaptic membrane exocytosis protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATTTG AGACACTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
TTCTCATCCC CACCAAATAT TTTACAAAAT GATCTTTTTG GACAAACGTT GAACAACACT 
AGGAAAAGAA GCCCATCAAT ATCAAGAGAA CAGAACAGAA GATACGACCA AAGGGACGAG 
AGAGACGAAT ACTCACAGTA TGCTACATCA GACAGTGCGA TGCCCAGGTC ACCATCAGAT 
TATTCCGATA GGCGGTCGCA GCGTGGGCCT CAGTTATATG AAGAGCCTGA ACTGGGTGAT 
TATAGAGATT CCAACAGAAG GAGTCGCAGG CGTTCCAAGG AGTATCCGGT AGAAGAGGAA 
GATGTACAAA ACAGGGAAGA ATATGAAAGG CAGCGGAGGG AAGAGGAATA CCAGGCACGG 
TACCGAAGCG ATCCAAATTT GGCTCGTTAT CCAGTCAAGC CGCAACCGTA TGAGGAACAA 
ATGCGTATCC ATGCTGAAGT GTCCCGGGCA CGTCATGAAC GGAGACATAG TGATGTCTCA 
TTGGCAAATA CTGAGTTGGA AGATTCTCGG ATGTCTATGC TGAGGATGGA ACGACCATCA 
AGACAAAGAT CAGTGTCTGA GCGTAGGGCT GCCATGGAAA ATCAGCGCTC ATACTCTATG 
GAAAGAACTC GAGAGGCTCA GGGACCAAGT CCCAATCGCC AGAGGACCAC AAATCATAGT 
CCTCCTACAC CTAGGAGGAG TCCAATTCCT CTGGAAAGAC CAGACATGAG GCGCTCTGAT 
TCATTAAGGA AACAACACCA TTTGGATCCC AATTCTGCTG TACGGAAAAC AAAACGTGAA 
AAGATGGAGA CCATGTTACG GAATGATTCG CTCAGCTCAG ACCAGTCTGA ATCTGTCAGA 
CCTCCACCAC CAAAGCCCCA TAAAACGAAA AAGGGAGGTA AAATGCGCCA GGTTTCATTA 
AGTAGTTCAG AAGAAGAATT GGCGTCCACT CCAGAGTATA CAAGTTGTGA TGATGTAGAG 
ATTGAAAGTG AAAGTGTTAG TGAAAAAGGA GACATGGATT ACAACTGGTT GGACCATACG 
TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCATC CAGTAACTTG GCAACCATCC 
AAAGATGGAG ATCGTCTCAT TGGTCGGATT TTGTTAAATA AACGCCTAAA AGATGGAAGT 
GTGCCTAGAG ACTCAGGAGC AATGCTTGGA TTGAAGGTAG TAGGAGGAAA GATGACTGAA 
TCAGGTCGAC TCTGCGCATT TATCACCAAA GTTAAAAAAG GAAGCTTAGC CGATACAGTG 
GGGCATCTCA GACCAGGTGA TGAAGTATTA GAATGGAATG GAAGGCTACT ACAAGGAGCC 
ACGTTTGAGG AAGTGTATAA CATCATTTTA GAATCCAAAC CTGAGCCACA AGTGGAGCTT 
GTTGTTTCAA GACCAATAGG GGACATTCCC AGAATACCTG ACAGCACACA TGCACAGCTG 
GAGTCCAGCT CTAGTTCATT TGAATCTCAA AAAATGGACC GACCTTCTAT TTCAGTGACT 
TCTCCTATGA GTCCTGGCAT GTTAAGGGAT GTTCCACAGT TCTTGTCTGG ACAACTTTCA 
AGCCAAAGCC TTAGTAGAAG AACAACGCCT TTTGTTCCTA GGGTTCAGAT AAAACTGTGG 
TATGACAAGG TTGGTCATCA GTTAATAGTG ACGATATTGG GAGCAAAGGA TCTTCCTTCA 
AGGGAAGATG GGAGGCCAAG GAATCCTTAT GTTAAAATTT ACTTTCTTCC AGACAGAAGT 
GATAAAAATA AAAGAAGAAC AAAAACAGTA AAGAAAACAT TGGAACCTAA GTGGAATCAG 
ACATTCATTT ATTCTCCAGT TCATCGGAGA GAGTTTAGAG AACGAATGCT AGAAATTACT 
CTTTGGGACC AAGCACGAGT TCGAGAGGAA GAAAGTGAAT TTTTAGGAGA GATTCTTATT 
GAGTTAGAGA CAGCTTTACT AGATGACGAA CCTCACTGGT ACAAACTTCA AACGCATGAT 
GTCTCTTCAT TGCCACTTCC CCATCCCTCA CCATATTTGC CACGCAGACA GCTCCATGGT 
GAGAGTCCCA CACGGAGGTT ACAAAATAAA GGCTTATATT CATATAATTC AGGGTCCAAA 
AGAATCAGCG ACAGTGAAGT CTCTGATTAT GACTGTGAAG ATGGAATTGG TGTTGTTTCA 
GATTATCGAC ATAATGGGAG AGATCTTCAA AGTTCAACGC TATCGGTGCC AGAACAAGTT 
ATGTCATCTA ATCATTGCTC TCGATCAGGG TCCCCTCACC GTGGAGATAG TATAGGACGG 
ACTAGATCGT GGTCTCCCAG TGTCCCTCCC CCACAAAGTC GGAATGTGGA TCAGGGACCA 
CGAGGGACTC GATCTACTCC TGCTCATTAC AATACCCTGA ACCGAATGGA TAGACACCGT 
GTCATAGATG ACCACTACTC CCCGGACAGA GAGAGGAATT GTGAAGCAGC AGATAGACAG 
CCATATCAAA GATCCAGATC AACAGAGCAA CGTCCTATGC TAGAGCGGAC CAACTCCCGC 
TCCCGATCCA CAGAGCGTCC TGACTCAAAC ATCATCAGGT CGATGCCTTC ATTAATGACT 
GGAAGATCTG CCCCTCCTTC ACCTGCCTTA CCGAGGTCAC ATCCTCGAAC TGGGTCTGTT 
CAGACAAGTC CATCAAGTAC TCCAGTAGTA GGGCGACGGG GCAGGCAGTT GCCGCAGCTG 
CCACCAAAGG GGACATTGGA GAGAAAAGAA GCGGAATCAT CAAGGAGAAG AAACTCAGAG 
GAATGCCCTG AGCCATTTAC AACCTTTGAA GAAAAGAAAG CAGATCCAGA GAATGGGGAT 
ACAGGTGCAA TGGATGTAGA AGAGAGGAAT CGCCAAATGA AAATGAGCAA GTACAAACAG 
GTAGCAGGAT CAGATTCCAG GCTGGAACAA GATTATCATT CTAAGTATCG GTCAGGACGG 
GATCCTCAGC GAGGCTCAGA CAACGTTTCC AATAAGTCTT CAGACAGCGA CGTCAGTGAC 
GTCTCCGCCG TGTCACGGAC AAGCAGCGCT TCACGTTTCA GCAGCACAAG CTACATGTCT 
GTCCAGTCGG AGCGGCCAAG GGGAAACAAA AAAATAAGTG TCTTTACATC CAAAATGCAA 
AGCAGACAGA TGGGCGCCTC AGGAAAGAAC ATGACTAAGA GCACCAGCAT CGGCGGGGAC 
ATGTACACGC TGGAGAAGAA CGACGGCAGC CAGTCAGACA CCGCAGTGGG CACTGTTGGT 
GGTGGTGGCA AAAAGAGACG CTCCAGCATC GGTGCAAAGA TGGTCGCCAT TGTGGGTCTC 
TCGCGAAAAA GCCGTAGCAC GTCACAACTC AGCCAAACTG AAGCAGGGGG CAAGAAGCTG 
CGGAGCACCG TCCAGAGGAG CACTGAGACA GGGCTGGCCG TGGAGATGAG GAACTGGATG 
ACCCGTCAGG CCAGCCGGGA GTCAACGGAT GGGAGCATGA ACAGCTACAG CTCTGAGGGA 
AATTTGATTT TCCCTGGTGT GAGGTTGGCT GCAGACAGCC AGTTCAGTGA TTTTCTGGAT 
GGACTTGGTC CTGCCCAGCT TGTAGGGCGA CAGACTTTGG CGACACCATC GATGGGAGAT 
ATTCAGGTGG GAATGATGGA CAAGAAAGGA CAATTGGAAG TAGAAATAAT CCGAGCTCGT 
GGCCTTGTTG TAAAACCAGG TTCAAAGACA CTGCCAGCAC CATATGTAAA GGTGTATCTA 
TTAGAAAATG GAGTCTGCAT AGCCAAAAAG AAAACAAAGG TAGCAAGAAA AACGCTGGAA 
CCGCTTTATC AGCAACTTCT GTCATTTGAA GAAAGTCCTC AAGGCAAAGT TTTACAGATA 
ATCGTTTGGG GAGACTATGG ACGTATGGAT CACAAGTCCT TTATGGGAGT GGCACAGATA 
CTTTTAGATG AACTGGACCT GTCCAACATG GTGATTGGGT GGTTCAAACT CTTCCCTCCT 
TCTTCCCTAG TAGACCCAAC TTTAGCTCCT CTCACTAGAA GAGCTTCCCA ATCATCTCTT 
GAAAGTTCAA CAGGACCTTC GTATGCTCGC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014821003.1
CDS	462..4577
Translation	MQFETLRQVCNSVLSHFHGVFSSPPNILQNDLFGQTLNNTRKRSPSISREQNRRYDQRDERDEYSQYATSDSAMPRSPSDYSDRRSQRGPQLYEEPELGDYRDSNRRSRRRSKEYPVEEEDVQNREEYERQRREEEYQARYRSDPNLARYPVKPQPYEEQMRIHAEVSRARHERRHSDVSLANTELEDSRMSMLRMERPSRQRSVSERRAAMENQRSYSMERTREAQGPSPNRQRTTNHSPPTPRRSPIPLERPDMRRSDSLRKQHHLDPNSAVRKTKREKMETMLRNDSLSSDQSESVRPPPPKPHKTKKGGKMRQVSLSSSEEELASTPEYTSCDDVEIESESVSEKGDMDYNWLDHTSWHSSEASPMSLHPVTWQPSKDGDRLIGRILLNKRLKDGSVPRDSGAMLGLKVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGATFEEVYNIILESKPEPQVELVVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRPSISVTSPMSPGMLRDVPQFLSGQLSSQSLSRRTTPFVPRVQIKLWYDKVGHQLIVTILGAKDLPSREDGRPRNPYVKIYFLPDRSDKNKRRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARVREEESEFLGEILIELETALLDDEPHWYKLQTHDVSSLPLPHPSPYLPRRQLHGESPTRRLQNKGLYSYNSGSKRISDSEVSDYDCEDGIGVVSDYRHNGRDLQSSTLSVPEQVMSSNHCSRSGSPHRGDSIGRTRSWSPSVPPPQSRNVDQGPRGTRSTPAHYNTLNRMDRHRVIDDHYSPDRERNCEAADRQPYQRSRSTEQRPMLERTNSRSRSTERPDSNIIRSMPSLMTGRSAPPSPALPRSHPRTGSVQTSPSSTPVVGRRGRQLPQLPPKGTLERKEAESSRRRNSEECPEPFTTFEEKKADPENGDTGAMDVEERNRQMKMSKYKQVAGSDSRLEQDYHSKYRSGRDPQRGSDNVSNKSSDSDVSDVSAVSRTSSASRFSSTSYMSVQSERPRGNKKISVFTSKMQSRQMGASGKNMTKSTSIGGDMYTLEKNDGSQSDTAVGTVGGGGKKRRSSIGAKMVAIVGLSRKSRSTSQLSQTEAGGKKLRSTVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLAADSQFSDFLDGLGPAQLVGRQTLATPSMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLENGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELDLSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYARS

Target ORF information:

RefSeq Version	XM_014965517.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014965517.1

ORF Insert Sequence:

ATGCAATTTG AGACACTGCG CCAGGTCTGC AATTCTGTTT TATCTCATTT TCATGGGGTT 
TTCTCATCCC CACCAAATAT TTTACAAAAT GATCTTTTTG GACAAACGTT GAACAACACT 
AGGAAAAGAA GCCCATCAAT ATCAAGAGAA CAGAACAGAA GATACGACCA AAGGGACGAG 
AGAGACGAAT ACTCACAGTA TGCTACATCA GACAGTGCGA TGCCCAGGTC ACCATCAGAT 
TATTCCGATA GGCGGTCGCA GCGTGGGCCT CAGTTATATG AAGAGCCTGA ACTGGGTGAT 
TATAGAGATT CCAACAGAAG GAGTCGCAGG CGTTCCAAGG AGTATCCGGT AGAAGAGGAA 
GATGTACAAA ACAGGGAAGA ATATGAAAGG CAGCGGAGGG AAGAGGAATA CCAGGCACGG 
TACCGAAGCG ATCCAAATTT GGCTCGTTAT CCAGTCAAGC CGCAACCGTA TGAGGAACAA 
ATGCGTATCC ATGCTGAAGT GTCCCGGGCA CGTCATGAAC GGAGACATAG TGATGTCTCA 
TTGGCAAATA CTGAGTTGGA AGATTCTCGG ATGTCTATGC TGAGGATGGA ACGACCATCA 
AGACAAAGAT CAGTGTCTGA GCGTAGGGCT GCCATGGAAA ATCAGCGCTC ATACTCTATG 
GAAAGAACTC GAGAGGCTCA GGGACCAAGT CCCAATCGCC AGAGGACCAC AAATCATAGT 
CCTCCTACAC CTAGGAGGAG TCCAATTCCT CTGGAAAGAC CAGACATGAG GCGCTCTGAT 
TCATTAAGGA AACAACACCA TTTGGATCCC AATTCTGCTG TACGGAAAAC AAAACGTGAA 
AAGATGGAGA CCATGTTACG GAATGATTCG CTCAGCTCAG ACCAGTCTGA ATCTGTCAGA 
CCTCCACCAC CAAAGCCCCA TAAAACGAAA AAGGGAGGTA AAATGCGCCA GGTTTCATTA 
AGTAGTTCAG AAGAAGAATT GGCGTCCACT CCAGAGTATA CAAGTTGTGA TGATGTAGAG 
ATTGAAAGTG AAAGTGTTAG TGAAAAAGGA GACATGGATT ACAACTGGTT GGACCATACG 
TCTTGGCATA GCAGTGAGGC ATCCCCAATG TCTTTGCATC CAGTAACTTG GCAACCATCC 
AAAGATGGAG ATCGTCTCAT TGGTCGGATT TTGTTAAATA AACGCCTAAA AGATGGAAGT 
GTGCCTAGAG ACTCAGGAGC AATGCTTGGA TTGAAGGTAG TAGGAGGAAA GATGACTGAA 
TCAGGTCGAC TCTGCGCATT TATCACCAAA GTTAAAAAAG GAAGCTTAGC CGATACAGTG 
GGGCATCTCA GACCAGGTGA TGAAGTATTA GAATGGAATG GAAGGCTACT ACAAGGAGCC 
ACGTTTGAGG AAGTGTATAA CATCATTTTA GAATCCAAAC CTGAGCCACA AGTGGAGCTT 
GTTGTTTCAA GACCAATAGG GGACATTCCC AGAATACCTG ACAGCACACA TGCACAGCTG 
GAGTCCAGCT CTAGTTCATT TGAATCTCAA AAAATGGACC GACCTTCTAT TTCAGTGACT 
TCTCCTATGA GTCCTGGCAT GTTAAGGGAT GTTCCACAGT TCTTGTCTGG ACAACTTTCA 
AGCCAAAGCC TTAGTAGAAG AACAACGCCT TTTGTTCCTA GGGTTCAGAT AAAACTGTGG 
TATGACAAGG TTGGTCATCA GTTAATAGTG ACGATATTGG GAGCAAAGGA TCTTCCTTCA 
AGGGAAGATG GGAGGCCAAG GAATCCTTAT GTTAAAATTT ACTTTCTTCC AGACAGAAGT 
GATAAAAATA AAAGAAGAAC AAAAACAGTA AAGAAAACAT TGGAACCTAA GTGGAATCAG 
ACATTCATTT ATTCTCCAGT TCATCGGAGA GAGTTTAGAG AACGAATGCT AGAAATTACT 
CTTTGGGACC AAGCACGAGT TCGAGAGGAA GAAAGTGAAT TTTTAGGAGA GATTCTTATT 
GAGTTAGAGA CAGCTTTACT AGATGACGAA CCTCACTGGT ACAAACTTCA AACGCATGAT 
GTCTCTTCAT TGCCACTTCC CCATCCCTCA CCATATTTGC CACGCAGACA GCTCCATGGT 
GAGAGTCCCA CACGGAGGTT ACAAAATAAA GGCTTATATT CATATAATTC AGGGTCCAAA 
AGAATCAGCG ACAGTGAAGT CTCTGATTAT GACTGTGAAG ATGGAATTGG TGTTGTTTCA 
GATTATCGAC ATAATGGGAG AGATCTTCAA AGTTCAACGC TATCGGTGCC AGAACAAGTT 
ATGTCATCTA ATCATTGCTC TCGATCAGGG TCCCCTCACC GTGGAGATAG TATAGGACGG 
ACTAGATCGT GGTCTCCCAG TGTCCCTCCC CCACAAAGTC GGAATGTGGA TCAGGGACCA 
CGAGGGACTC GATCTACTCC TGCTCATTAC AATACCCTGA ACCGAATGGA TAGACACCGT 
GTCATAGATG ACCACTACTC CCCGGACAGA GAGAGGAATT GTGAAGCAGC AGATAGACAG 
CCATATCAAA GATCCAGATC AACAGAGCAA CGTCCTATGC TAGAGCGGAC CAACTCCCGC 
TCCCGATCCA CAGAGCGTCC TGACTCAAAC ATCATCAGGT CGATGCCTTC ATTAATGACT 
GGAAGATCTG CCCCTCCTTC ACCTGCCTTA CCGAGGTCAC ATCCTCGAAC TGGGTCTGTT 
CAGACAAGTC CATCAAGTAC TCCAGTAGTA GGGCGACGGG GCAGGCAGTT GCCGCAGCTG 
CCACCAAAGG GGACATTGGA GAGAAAAGAA GCGGAATCAT CAAGGAGAAG AAACTCAGAG 
GAATGCCCTG AGCCATTTAC AACCTTTGAA GAAAAGAAAG CAGATCCAGA GAATGGGGAT 
ACAGGTGCAA TGGATGTAGA AGAGAGGAAT CGCCAAATGA AAATGAGCAA GTACAAACAG 
GTAGCAGGAT CAGATTCCAG GCTGGAACAA GATTATCATT CTAAGTATCG GTCAGGACGG 
GATCCTCAGC GAGGCTCAGA CAACGTTTCC AATAAGTCTT CAGACAGCGA CGTCAGTGAC 
GTCTCCGCCG TGTCACGGAC AAGCAGCGCT TCACGTTTCA GCAGCACAAG CTACATGTCT 
GTCCAGTCGG AGCGGCCAAG GGGAAACAAA AAAATAAGTG TCTTTACATC CAAAATGCAA 
AGCAGACAGA TGGGCGCCTC AGGAAAGAAC ATGACTAAGA GCACCAGCAT CGGCGGGGAC 
ATGTACACGC TGGAGAAGAA CGACGGCAGC CAGTCAGACA CCGCAGTGGG CACTGTTGGT 
GGTGGTGGCA AAAAGAGACG CTCCAGCATC GGTGCAAAGA TGGTCGCCAT TGTGGGTCTC 
TCGCGAAAAA GCCGTAGCAC GTCACAACTC AGCCAAACTG AAGCAGGGGG CAAGAAGCTG 
CGGAGCACCG TCCAGAGGAG CACTGAGACA GGGCTGGCCG TGGAGATGAG GAACTGGATG 
ACCCGTCAGG CCAGCCGGGA GTCAACGGAT GGGAGCATGA ACAGCTACAG CTCTGAGGGA 
AATTTGATTT TCCCTGGTGT GAGGTTGGCT GCAGACAGCC AGTTCAGTGA TTTTCTGGAT 
GGACTTGGTC CTGCCCAGCT TGTAGGGCGA CAGACTTTGG CGACACCATC GATGGGAGAT 
ATTCAGGTGG GAATGATGGA CAAGAAAGGA CAATTGGAAG TAGAAATAAT CCGAGCTCGT 
GGCCTTGTTG TAAAACCAGG TTCAAAGACA CTGCCAGCAC CATATGTAAA GGTGTATCTA 
TTAGAAAATG GAGTCTGCAT AGCCAAAAAG AAAACAAAGG TAGCAAGAAA AACGCTGGAA 
CCGCTTTATC AGCAACTTCT GTCATTTGAA GAAAGTCCTC AAGGCAAAGT TTTACAGATA 
ATCGTTTGGG GAGACTATGG ACGTATGGAT CACAAGTCCT TTATGGGAGT GGCACAGATA 
CTTTTAGATG AACTGGACCT GTCCAACATG GTGATTGGGT GGTTCAAACT CTTCCCTCCT 
TCTTCCCTAG TAGACCCAAC TTTAGCTCCT CTCACTAGAA GAGCTTCCCA ATCATCTCTT 
GAAAGTTCAA CAGGACCTTC GTATGCTCGC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIMS2 ( XM_014965518.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821004.1 Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa81971
Clone ID Related Accession (Same CDS sequence)	XM_014965518.1
Accession Version	XM_014965518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	regulating synaptic membrane exocytosis protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAGGT CACCATCAGA TTATTCCGAT AGGCGGTCGC AGCGTGGGCC TCAGTTATAT 
GAAGAGCCTG AACTGGGTGA TTATAGAGAT TCCAACAGAA GGAGTCGCAG GCGTTCCAAG 
GAGTATCCGG TAGAAGAGGA AGATGTACAA AACAGGGAAG AATATGAAAG GCAGCGGAGG 
GAAGAGGAAT ACCAGGCACG GTACCGAAGC GATCCAAATT TGGCTCGTTA TCCAGTCAAG 
CCGCAACCGT ATGAGGAACA AATGCGTATC CATGCTGAAG TGTCCCGGGC ACGTCATGAA 
CGGAGACATA GTGATGTCTC ATTGGCAAAT ACTGAGTTGG AAGATTCTCG GATGTCTATG 
CTGAGGATGG AACGACCATC AAGACAAAGA TCAGTGTCTG AGCGTAGGGC TGCCATGGAA 
AATCAGCGCT CATACTCTAT GGAAAGAACT CGAGAGGCTC AGGGACCAAG TCCCAATCGC 
CAGAGGACCA CAAATCATAG TCCTCCTACA CCTAGGAGGA GTCCAATTCC TCTGGAAAGA 
CCAGACATGA GGCGCTCTGA TTCATTAAGG AAACAACACC ATTTGGATCC CAATTCTGCT 
GTACGGAAAA CAAAACGTGA AAAGATGGAG ACCATGTTAC GGAATGATTC GCTCAGCTCA 
GACCAGTCTG AATCTGTCAG ACCTCCACCA CCAAAGCCCC ATAAAACGAA AAAGGGAGGT 
AAAATGCGCC AGGTTTCATT AAGTAGTTCA GAAGAAGAAT TGGCGTCCAC TCCAGAGTAT 
ACAAGTTGTG ATGATGTAGA GATTGAAAGT GAAAGTGTTA GTGAAAAAGG GGACAGTCAA 
AGGGGAAAAA GAAAAACTAG TGAGCAGGCA GTTTTGTCGG ATTCTAACAC CTTATCCCAG 
AGGCAAAAGA AAATGGTGTG CTTTGGTGGC CACTCTTTGG AAGAGGACTT GGAATGGTCT 
GAGCCTCAGA TAAAAGACTC TGGGGTAGAT ACGTGTAGTA GCACAACTCT AAACGAGGAA 
CATAGCCATA GCGAGAAGCA TCCAGTAACT TGGCAACCAT CCAAAGATGG AGATCGTCTC 
ATTGGTCGGA TTTTGTTAAA TAAACGCCTA AAAGATGGAA GTGTGCCTAG AGACTCAGGA 
GCAATGCTTG GATTGAAGGT AGTAGGAGGA AAGATGACTG AATCAGGTCG ACTCTGCGCA 
TTTATCACCA AAGTTAAAAA AGGAAGCTTA GCCGATACAG TGGGGCATCT CAGACCAGGT 
GATGAAGTAT TAGAATGGAA TGGAAGGCTA CTACAAGGAG CCACGTTTGA GGAAGTGTAT 
AACATCATTT TAGAATCCAA ACCTGAGCCA CAAGTGGAGC TTGTTGTTTC AAGACCAATA 
GGGGACATTC CCAGAATACC TGACAGCACA CATGCACAGC TGGAGTCCAG CTCTAGTTCA 
TTTGAATCTC AAAAAATGGA CCGACCTTCT ATTTCAGTGA CTTCTCCTAT GAGTCCTGGC 
ATGTTAAGGG ATGTTCCACA GTTCTTGTCT GGACAACTTT CAAGCCAAAG CCTTAGTAGA 
AGAACAACGC CTTTTGTTCC TAGGGTTCAG ATAAAACTGT GGTATGACAA GGTTGGTCAT 
CAGTTAATAG TGACGATATT GGGAGCAAAG GATCTTCCTT CAAGGGAAGA TGGGAGGCCA 
AGGAATCCTT ATGTTAAAAT TTACTTTCTT CCAGACAGAA GTGATAAAAA TAAAAGAAGA 
ACAAAAACAG TAAAGAAAAC ATTGGAACCT AAGTGGAATC AGACATTCAT TTATTCTCCA 
GTTCATCGGA GAGAGTTTAG AGAACGAATG CTAGAAATTA CTCTTTGGGA CCAAGCACGA 
GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA GAGATTCTTA TTGAGTTAGA GACAGCTTTA 
CTAGATGACG AACCTCACTG GTACAAACTT CAAACGCATG ATGTCTCTTC ATTGCCACTT 
CCCCATCCCT CACCATATTT GCCACGCAGA CAGCTCCATG GTGAGAGTCC CACACGGAGG 
TTACAAAATA AAGGCTTATA TTCATATAAT TCAGGGTCCA AAAGAATCAG CGACAGTGAA 
GTCTCTGATT ATGACTGTGA AGATGGAATT GGTGTTGTTT CAGATTATCG ACATAATGGG 
AGAGATCTTC AAAGTTCAAC GCTATCGGTG CCAGAACAAG TTATGTCATC TAATCATTGC 
TCTCGATCAG GGTCCCCTCA CCGTGGAGAT AGTATAGGAC GGACTAGATC GTGGTCTCCC 
AGTGTCCCTC CCCCACAAAG TCGGAATGTG GATCAGGGAC CACGAGGGAC TCGATCTACT 
CCTGCTCATT ACAATACCCT GAACCGAATG GATAGACACC GTGTCATAGA TGACCACTAC 
TCCCCGGACA GAGAGAGGAA TTGTGAAGCA GCAGATAGAC AGCCATATCA AAGATCCAGA 
TCAACAGAGC AACGTCCTAT GCTAGAGCGG ACCAACTCCC GCTCCCGATC CACAGAGCGT 
CCTGACTCAA ACATCATCAG GTCGATGCCT TCATTAATGA CTGGAAGATC TGCCCCTCCT 
TCACCTGCCT TACCGAGGTC ACATCCTCGA ACTGGGTCTG TTCAGACAAG TCCATCAAGT 
ACTCCAGTAG TAGGGCGACG GGGCAGGCAG TTGCCGCAGC TGCCACCAAA GGGGACATTG 
GAGAGAAAAG AAGCGGAATC ATCAAGGAGA AGAAACTCAG AGGAATGCCC TGAGCCATTT 
ACAACCTTTG AAGAAAAGAA AGCAGATCCA GAGAATGGGG ATACAGGTGC AATGGATGTA 
GAAGAGAGGA ATCGCCAAAT GAAAATGAGC AAGTACAAAC AGGTAGCAGG ATCAGATTCC 
AGGCTGGAAC AAGATTATCA TTCTAAGTAT CGGTCAGGAC GGGATCCTCA GCGAGGCTCA 
GACAACGTTT CCAATAAGTC TTCAGACAGC GACGTCAGTG ACGTCTCCGC CGTGTCACGG 
ACAAGCAGCG CTTCACGTTT CAGCAGCACA AGCTACATGT CTGTCCAGTC GGAGCGGCCA 
AGGGGAAACA AAAAAATAAG TGTCTTTACA TCCAAAATGC AAAGCAGACA GATGGGCGCC 
TCAGGAAAGA ACATGACTAA GAGCACCAGC ATCGGCGGGG ACATGTACAC GCTGGAGAAG 
AACGACGGCA GCCAGTCAGA CACCGCAGTG GGCACTGTTG GTGGTGGTGG CAAAAAGAGA 
CGCTCCAGCA TCGGTGCAAA GATGGTCGCC ATTGTGGGTC TCTCGCGAAA AAGCCGTAGC 
ACGTCACAAC TCAGCCAAAC TGAAGCAGGG GGCAAGAAGC TGCGGAGCAC CGTCCAGAGG 
AGCACTGAGA CAGGGCTGGC CGTGGAGATG AGGAACTGGA TGACCCGTCA GGCCAGCCGG 
GAGTCAACGG ATGGGAGCAT GAACAGCTAC AGCTCTGAGG GAAATTTGAT TTTCCCTGGT 
GTGAGGTTGG CTGCAGACAG CCAGTTCAGT GATTTTCTGG ATGGACTTGG TCCTGCCCAG 
CTTGTAGGGC GACAGACTTT GGCGACACCA TCGATGGGAG ATATTCAGGT GGGAATGATG 
GACAAGAAAG GACAATTGGA AGTAGAAATA ATCCGAGCTC GTGGCCTTGT TGTAAAACCA 
GGTTCAAAGA CACTGCCAGC ACCATATGTA AAGGTGTATC TATTAGAAAA TGGAGTCTGC 
ATAGCCAAAA AGAAAACAAA GGTAGCAAGA AAAACGCTGG AACCGCTTTA TCAGCAACTT 
CTGTCATTTG AAGAAAGTCC TCAAGGCAAA GTTTTACAGA TAATCGTTTG GGGAGACTAT 
GGACGTATGG ATCACAAGTC CTTTATGGGA GTGGCACAGA TACTTTTAGA TGAACTGGAC 
CTGTCCAACA TGGTGATTGG GTGGTTCAAA CTCTTCCCTC CTTCTTCCCT AGTAGACCCA 
ACTTTAGCTC CTCTCACTAG AAGAGCTTCC CAATCATCTC TTGAAAGTTC AACAGGACCT 
TCGTATGCTC GCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014821004.1
CDS	210..4247
Translation	MPRSPSDYSDRRSQRGPQLYEEPELGDYRDSNRRSRRRSKEYPVEEEDVQNREEYERQRREEEYQARYRSDPNLARYPVKPQPYEEQMRIHAEVSRARHERRHSDVSLANTELEDSRMSMLRMERPSRQRSVSERRAAMENQRSYSMERTREAQGPSPNRQRTTNHSPPTPRRSPIPLERPDMRRSDSLRKQHHLDPNSAVRKTKREKMETMLRNDSLSSDQSESVRPPPPKPHKTKKGGKMRQVSLSSSEEELASTPEYTSCDDVEIESESVSEKGDSQRGKRKTSEQAVLSDSNTLSQRQKKMVCFGGHSLEEDLEWSEPQIKDSGVDTCSSTTLNEEHSHSEKHPVTWQPSKDGDRLIGRILLNKRLKDGSVPRDSGAMLGLKVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGATFEEVYNIILESKPEPQVELVVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRPSISVTSPMSPGMLRDVPQFLSGQLSSQSLSRRTTPFVPRVQIKLWYDKVGHQLIVTILGAKDLPSREDGRPRNPYVKIYFLPDRSDKNKRRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARVREEESEFLGEILIELETALLDDEPHWYKLQTHDVSSLPLPHPSPYLPRRQLHGESPTRRLQNKGLYSYNSGSKRISDSEVSDYDCEDGIGVVSDYRHNGRDLQSSTLSVPEQVMSSNHCSRSGSPHRGDSIGRTRSWSPSVPPPQSRNVDQGPRGTRSTPAHYNTLNRMDRHRVIDDHYSPDRERNCEAADRQPYQRSRSTEQRPMLERTNSRSRSTERPDSNIIRSMPSLMTGRSAPPSPALPRSHPRTGSVQTSPSSTPVVGRRGRQLPQLPPKGTLERKEAESSRRRNSEECPEPFTTFEEKKADPENGDTGAMDVEERNRQMKMSKYKQVAGSDSRLEQDYHSKYRSGRDPQRGSDNVSNKSSDSDVSDVSAVSRTSSASRFSSTSYMSVQSERPRGNKKISVFTSKMQSRQMGASGKNMTKSTSIGGDMYTLEKNDGSQSDTAVGTVGGGGKKRRSSIGAKMVAIVGLSRKSRSTSQLSQTEAGGKKLRSTVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLAADSQFSDFLDGLGPAQLVGRQTLATPSMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLENGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELDLSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYARS

Target ORF information:

RefSeq Version	XM_014965518.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014965518.1

ORF Insert Sequence:

ATGCCCAGGT CACCATCAGA TTATTCCGAT AGGCGGTCGC AGCGTGGGCC TCAGTTATAT 
GAAGAGCCTG AACTGGGTGA TTATAGAGAT TCCAACAGAA GGAGTCGCAG GCGTTCCAAG 
GAGTATCCGG TAGAAGAGGA AGATGTACAA AACAGGGAAG AATATGAAAG GCAGCGGAGG 
GAAGAGGAAT ACCAGGCACG GTACCGAAGC GATCCAAATT TGGCTCGTTA TCCAGTCAAG 
CCGCAACCGT ATGAGGAACA AATGCGTATC CATGCTGAAG TGTCCCGGGC ACGTCATGAA 
CGGAGACATA GTGATGTCTC ATTGGCAAAT ACTGAGTTGG AAGATTCTCG GATGTCTATG 
CTGAGGATGG AACGACCATC AAGACAAAGA TCAGTGTCTG AGCGTAGGGC TGCCATGGAA 
AATCAGCGCT CATACTCTAT GGAAAGAACT CGAGAGGCTC AGGGACCAAG TCCCAATCGC 
CAGAGGACCA CAAATCATAG TCCTCCTACA CCTAGGAGGA GTCCAATTCC TCTGGAAAGA 
CCAGACATGA GGCGCTCTGA TTCATTAAGG AAACAACACC ATTTGGATCC CAATTCTGCT 
GTACGGAAAA CAAAACGTGA AAAGATGGAG ACCATGTTAC GGAATGATTC GCTCAGCTCA 
GACCAGTCTG AATCTGTCAG ACCTCCACCA CCAAAGCCCC ATAAAACGAA AAAGGGAGGT 
AAAATGCGCC AGGTTTCATT AAGTAGTTCA GAAGAAGAAT TGGCGTCCAC TCCAGAGTAT 
ACAAGTTGTG ATGATGTAGA GATTGAAAGT GAAAGTGTTA GTGAAAAAGG GGACAGTCAA 
AGGGGAAAAA GAAAAACTAG TGAGCAGGCA GTTTTGTCGG ATTCTAACAC CTTATCCCAG 
AGGCAAAAGA AAATGGTGTG CTTTGGTGGC CACTCTTTGG AAGAGGACTT GGAATGGTCT 
GAGCCTCAGA TAAAAGACTC TGGGGTAGAT ACGTGTAGTA GCACAACTCT AAACGAGGAA 
CATAGCCATA GCGAGAAGCA TCCAGTAACT TGGCAACCAT CCAAAGATGG AGATCGTCTC 
ATTGGTCGGA TTTTGTTAAA TAAACGCCTA AAAGATGGAA GTGTGCCTAG AGACTCAGGA 
GCAATGCTTG GATTGAAGGT AGTAGGAGGA AAGATGACTG AATCAGGTCG ACTCTGCGCA 
TTTATCACCA AAGTTAAAAA AGGAAGCTTA GCCGATACAG TGGGGCATCT CAGACCAGGT 
GATGAAGTAT TAGAATGGAA TGGAAGGCTA CTACAAGGAG CCACGTTTGA GGAAGTGTAT 
AACATCATTT TAGAATCCAA ACCTGAGCCA CAAGTGGAGC TTGTTGTTTC AAGACCAATA 
GGGGACATTC CCAGAATACC TGACAGCACA CATGCACAGC TGGAGTCCAG CTCTAGTTCA 
TTTGAATCTC AAAAAATGGA CCGACCTTCT ATTTCAGTGA CTTCTCCTAT GAGTCCTGGC 
ATGTTAAGGG ATGTTCCACA GTTCTTGTCT GGACAACTTT CAAGCCAAAG CCTTAGTAGA 
AGAACAACGC CTTTTGTTCC TAGGGTTCAG ATAAAACTGT GGTATGACAA GGTTGGTCAT 
CAGTTAATAG TGACGATATT GGGAGCAAAG GATCTTCCTT CAAGGGAAGA TGGGAGGCCA 
AGGAATCCTT ATGTTAAAAT TTACTTTCTT CCAGACAGAA GTGATAAAAA TAAAAGAAGA 
ACAAAAACAG TAAAGAAAAC ATTGGAACCT AAGTGGAATC AGACATTCAT TTATTCTCCA 
GTTCATCGGA GAGAGTTTAG AGAACGAATG CTAGAAATTA CTCTTTGGGA CCAAGCACGA 
GTTCGAGAGG AAGAAAGTGA ATTTTTAGGA GAGATTCTTA TTGAGTTAGA GACAGCTTTA 
CTAGATGACG AACCTCACTG GTACAAACTT CAAACGCATG ATGTCTCTTC ATTGCCACTT 
CCCCATCCCT CACCATATTT GCCACGCAGA CAGCTCCATG GTGAGAGTCC CACACGGAGG 
TTACAAAATA AAGGCTTATA TTCATATAAT TCAGGGTCCA AAAGAATCAG CGACAGTGAA 
GTCTCTGATT ATGACTGTGA AGATGGAATT GGTGTTGTTT CAGATTATCG ACATAATGGG 
AGAGATCTTC AAAGTTCAAC GCTATCGGTG CCAGAACAAG TTATGTCATC TAATCATTGC 
TCTCGATCAG GGTCCCCTCA CCGTGGAGAT AGTATAGGAC GGACTAGATC GTGGTCTCCC 
AGTGTCCCTC CCCCACAAAG TCGGAATGTG GATCAGGGAC CACGAGGGAC TCGATCTACT 
CCTGCTCATT ACAATACCCT GAACCGAATG GATAGACACC GTGTCATAGA TGACCACTAC 
TCCCCGGACA GAGAGAGGAA TTGTGAAGCA GCAGATAGAC AGCCATATCA AAGATCCAGA 
TCAACAGAGC AACGTCCTAT GCTAGAGCGG ACCAACTCCC GCTCCCGATC CACAGAGCGT 
CCTGACTCAA ACATCATCAG GTCGATGCCT TCATTAATGA CTGGAAGATC TGCCCCTCCT 
TCACCTGCCT TACCGAGGTC ACATCCTCGA ACTGGGTCTG TTCAGACAAG TCCATCAAGT 
ACTCCAGTAG TAGGGCGACG GGGCAGGCAG TTGCCGCAGC TGCCACCAAA GGGGACATTG 
GAGAGAAAAG AAGCGGAATC ATCAAGGAGA AGAAACTCAG AGGAATGCCC TGAGCCATTT 
ACAACCTTTG AAGAAAAGAA AGCAGATCCA GAGAATGGGG ATACAGGTGC AATGGATGTA 
GAAGAGAGGA ATCGCCAAAT GAAAATGAGC AAGTACAAAC AGGTAGCAGG ATCAGATTCC 
AGGCTGGAAC AAGATTATCA TTCTAAGTAT CGGTCAGGAC GGGATCCTCA GCGAGGCTCA 
GACAACGTTT CCAATAAGTC TTCAGACAGC GACGTCAGTG ACGTCTCCGC CGTGTCACGG 
ACAAGCAGCG CTTCACGTTT CAGCAGCACA AGCTACATGT CTGTCCAGTC GGAGCGGCCA 
AGGGGAAACA AAAAAATAAG TGTCTTTACA TCCAAAATGC AAAGCAGACA GATGGGCGCC 
TCAGGAAAGA ACATGACTAA GAGCACCAGC ATCGGCGGGG ACATGTACAC GCTGGAGAAG 
AACGACGGCA GCCAGTCAGA CACCGCAGTG GGCACTGTTG GTGGTGGTGG CAAAAAGAGA 
CGCTCCAGCA TCGGTGCAAA GATGGTCGCC ATTGTGGGTC TCTCGCGAAA AAGCCGTAGC 
ACGTCACAAC TCAGCCAAAC TGAAGCAGGG GGCAAGAAGC TGCGGAGCAC CGTCCAGAGG 
AGCACTGAGA CAGGGCTGGC CGTGGAGATG AGGAACTGGA TGACCCGTCA GGCCAGCCGG 
GAGTCAACGG ATGGGAGCAT GAACAGCTAC AGCTCTGAGG GAAATTTGAT TTTCCCTGGT 
GTGAGGTTGG CTGCAGACAG CCAGTTCAGT GATTTTCTGG ATGGACTTGG TCCTGCCCAG 
CTTGTAGGGC GACAGACTTT GGCGACACCA TCGATGGGAG ATATTCAGGT GGGAATGATG 
GACAAGAAAG GACAATTGGA AGTAGAAATA ATCCGAGCTC GTGGCCTTGT TGTAAAACCA 
GGTTCAAAGA CACTGCCAGC ACCATATGTA AAGGTGTATC TATTAGAAAA TGGAGTCTGC 
ATAGCCAAAA AGAAAACAAA GGTAGCAAGA AAAACGCTGG AACCGCTTTA TCAGCAACTT 
CTGTCATTTG AAGAAAGTCC TCAAGGCAAA GTTTTACAGA TAATCGTTTG GGGAGACTAT 
GGACGTATGG ATCACAAGTC CTTTATGGGA GTGGCACAGA TACTTTTAGA TGAACTGGAC 
CTGTCCAACA TGGTGATTGG GTGGTTCAAA CTCTTCCCTC CTTCTTCCCT AGTAGACCCA 
ACTTTAGCTC CTCTCACTAG AAGAGCTTCC CAATCATCTC TTGAAAGTTC AACAGGACCT 
TCGTATGCTC GCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIMS2 ( XM_014965515.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821001.1 Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa81968
Clone ID Related Accession (Same CDS sequence)	XM_014965515.1
Accession Version	XM_014965515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	regulating synaptic membrane exocytosis protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACC TCAGCCACCT CACGGAGGAA GAGCGGAAGA TCATTCTGGC CGTCATGGAC 
CGCCAGAAGA AGGAAGAGGA GAAGGAGCAA TCCGTGCTCA AAAAATTACA TCAGCAGTTT 
GAAATGTATA AGGAGCAAGT GAAGAAGATG GGTGAAGAGT CACAACAGCA GCAGGAGCAG 
AAGGGTGATG CCCCAACGTG TGGCATCTGC CACAAAACAA AATTTGCTGA TGGCTGTGGC 
CATAACTGTT CATATTGCCA AACTAAATTC TGTGCACGCT GTGGAGGAAG AGTGTCTTTA 
CGGTCAAATA AGGAGGACAA AGTGGTAATG TGGGTATGTA ACTTGTGCCG AAAACAACAA 
GAAATCCTCA CAAAATCAGG AGCTTGGTTC TATAACAGTG GATCAAATGC ACCACAGCAG 
CCTGACCAAG AAGGTACTAG AGGTCTGCGG AACGAGGAAG CACCTCAAGA GAAAAAAGCA 
AAGCTGCAGG AGCATTCCCA GTACCAAGGA CCATCAGGTG ATGTATCCAC ACAAGTTTTG 
GACAAAAACA GATCTCAAGG GCTCACAAGA CAAGACTCAA TTAAGAATGG GTCAGGAGTA 
AAGCATCAAG TAACAAGTGA CACAACATCA GACAGGAAAA GAAGCCCATC AATATCAAGA 
GAACAGAACA GAAGATACGA CCAAAGGGAC GAGAGAGACG AATACTCACA GTATGCTACA 
TCAGACAGTG CGATGCCCAG GTCACCATCA GATTATTCCG ATAGGCGGTC GCAGCGTGGG 
CCTCAGTTAT ATGAAGAGCC TGAACTGGGT GATTATAGAG ATTCCAACAG AAGGAGTCGC 
AGGCGTTCCA AGGAGTATCC GGTAGAAGAG GAAGATGTAC AAAACAGGGA AGAATATGAA 
AGGCAGCGGA GGGAAGAGGA ATACCAGGCA CGGTACCGAA GCGATCCAAA TTTGGCTCGT 
TATCCAGTCA AGCCGCAACC GTATGAGGAA CAAATGCGTA TCCATGCTGA AGTGTCCCGG 
GCACGTCATG AACGGAGACA TAGTGATGTC TCATTGGCAA ATACTGAGTT GGAAGATTCT 
CGGATGTCTA TGCTGAGGAT GGAACGACCA TCAAGACAAA GATCAGTGTC TGAGCGTAGG 
GCTGCCATGG AAAATCAGCG CTCATACTCT ATGGAAAGAA CTCGAGAGGC TCAGGGACCA 
AGTCCCAATC GCCAGAGGAC CACAAATCAT AGTCCTCCTA CACCTAGGAG GAGTCCAATT 
CCTCTGGAAA GACCAGACAT GAGGCGCTCT GATTCATTAA GGAAACAACA CCATTTGGAT 
CCCAATTCTG CTGTACGGAA AACAAAACGT GAAAAGATGG AGACCATGTT ACGGAATGAT 
TCGCTCAGCT CAGACCAGTC TGAATCTGTC AGACCTCCAC CACCAAAGCC CCATAAAACG 
AAAAAGGGAG GTAAAATGCG CCAGGTTTCA TTAAGTAGTT CAGAAGAAGA ATTGGCGTCC 
ACTCCAGAGT ATACAAGTTG TGATGATGTA GAGATTGAAA GTGAAAGTGT TAGTGAAAAA 
GGGGACAGTC AAAGGGGAAA AAGAAAAACT AGTGAGCAGG CAGTTTTGTC GGATTCTAAC 
ACCTTATCCC AGAGGCAAAA GAAAATGGTG TGCTTTGGTG GCCACTCTTT GGAAGAGGAC 
TTGGAATGGT CTGAGCCTCA GATAAAAGAC TCTGGGGTAG ATACGTGTAG TAGCACAACT 
CTAAACGAGG AACATAGCCA TAGCGAGAAG CATCCAGTAA CTTGGCAACC ATCCAAAGAT 
GGAGATCGTC TCATTGGTCG GATTTTGTTA AATAAACGCC TAAAAGATGG AAGTGTGCCT 
AGAGACTCAG GAGCAATGCT TGGATTGAAG GTAGTAGGAG GAAAGATGAC TGAATCAGGT 
CGACTCTGCG CATTTATCAC CAAAGTTAAA AAAGGAAGCT TAGCCGATAC AGTGGGGCAT 
CTCAGACCAG GTGATGAAGT ATTAGAATGG AATGGAAGGC TACTACAAGG AGCCACGTTT 
GAGGAAGTGT ATAACATCAT TTTAGAATCC AAACCTGAGC CACAAGTGGA GCTTGTTGTT 
TCAAGACCAA TAGGGGACAT TCCCAGAATA CCTGACAGCA CACATGCACA GCTGGAGTCC 
AGCTCTAGTT CATTTGAATC TCAAAAAATG GACCGACCTT CTATTTCAGT GACTTCTCCT 
ATGAGTCCTG GCATGTTAAG GGATGTTCCA CAGTTCTTGT CTGGACAACT TTCAAGCCAA 
AGCCTTAGTA GAAGAACAAC GCCTTTTGTT CCTAGGGTTC AGATAAAACT GTGGTATGAC 
AAGGTTGGTC ATCAGTTAAT AGTGACGATA TTGGGAGCAA AGGATCTTCC TTCAAGGGAA 
GATGGGAGGC CAAGGAATCC TTATGTTAAA ATTTACTTTC TTCCAGACAG AAGTGATAAA 
AATAAAAGAA GAACAAAAAC AGTAAAGAAA ACATTGGAAC CTAAGTGGAA TCAGACATTC 
ATTTATTCTC CAGTTCATCG GAGAGAGTTT AGAGAACGAA TGCTAGAAAT TACTCTTTGG 
GACCAAGCAC GAGTTCGAGA GGAAGAAAGT GAATTTTTAG GAGAGATTCT TATTGAGTTA 
GAGACAGCTT TACTAGATGA CGAACCTCAC TGGTACAAAC TTCAAACGCA TGATGTCTCT 
TCATTGCCAC TTCCCCATCC CTCACCATAT TTGCCACGCA GACAGCTCCA TGGTGAGAGT 
CCCACACGGA GGTTACAAAA TAAAGGCTTA TATTCATATA ATTCAGGGTC CAAAAGAATC 
AGCGACAGTG AAGTCTCTGA TTATGACTGT GAAGATGGAA TTGGTGTTGT TTCAGATTAT 
CGACATAATG GGAGAGATCT TCAAAGTTCA ACGCTATCGG TGCCAGAACA AGTTATGTCA 
TCTAATCATT GCTCTCGATC AGGGTCCCCT CACCGTGGAG ATAGTATAGG ACGGACTAGA 
TCGTGGTCTC CCAGTGTCCC TCCCCCACAA AGTCGGAATG TGGATCAGGG ACCACGAGGG 
ACTCGATCTA CTCCTGCTCA TTACAATACC CTGAACCGAA TGGATAGACA CCGTGTCATA 
GATGACCACT ACTCCCCGGA CAGAGAGAGG AATTGTGAAG CAGCAGATAG ACAGCCATAT 
CAAAGATCCA GATCAACAGA GCAACGTCCT ATGCTAGAGC GGACCAACTC CCGCTCCCGA 
TCCACAGAGC GTCCTGACTC AAACATCATC AGGTCGATGC CTTCATTAAT GACTGGAAGA 
TCTGCCCCTC CTTCACCTGC CTTACCGAGG TCACATCCTC GAACTGGGTC TGTTCAGACA 
AGTCCATCAA GTACTCCAGT AGTAGGGCGA CGGGGCAGGC AGTTGCCGCA GCTGCCACCA 
AAGGGGACAT TGGAGAGAAA AGAAGCGGAA TCATCAAGGA GAAGAAACTC AGAGGAATGC 
CCTGAGCCAT TTACAACCTT TGAAGAAAAG AAAGCAGATC CAGAGAATGG GGATACAGGT 
GCAATGGATG TAGAAGAGAG GAATCGCCAA ATGAAAATGA GCAAGTACAA ACAGGTAGCA 
GGATCAGATT CCAGGCTGGA ACAAGATTAT CATTCTAAGT ATCGGTCAGG ACGGGATCCT 
CAGCGAGGCT CAGACAACGT TTCCAATAAG TCTTCAGACA GCGACGTCAG TGACGTCTCC 
GCCGTGTCAC GGACAAGCAG CGCTTCACGT TTCAGCAGCA CAAGCTACAT GTCTGTCCAG 
TCGGAGCGGC CAAGGGGAAA CAAAAAAATA AGTGTCTTTA CATCCAAAAT GCAAAGCAGA 
CAGATGGGCG CCTCAGGAAA GAACATGACT AAGAGCACCA GCATCGGCGG GGACATGTAC 
ACGCTGGAGA AGAACGACGG CAGCCAGTCA GACACCGCAG TGGGCACTGT TGGTGGTGGT 
GGCAAAAAGA GACGCTCCAG CATCGGTGCA AAGATGGTCG CCATTGTGGG TCTCTCGCGA 
AAAAGCCGTA GCACGTCACA ACTCAGCCAA ACTGAAGCAG GGGGCAAGAA GCTGCGGAGC 
ACCGTCCAGA GGAGCACTGA GACAGGGCTG GCCGTGGAGA TGAGGAACTG GATGACCCGT 
CAGGCCAGCC GGGAGTCAAC GGATGGGAGC ATGAACAGCT ACAGCTCTGA GGGAAATTTG 
ATTTTCCCTG GTGTGAGGTT GGCTGCAGAC AGCCAGTTCA GTGATTTTCT GGATGGACTT 
GGTCCTGCCC AGCTTGTAGG GCGACAGACT TTGGCGACAC CATCGATGGG AGATATTCAG 
GTGGGAATGA TGGACAAGAA AGGACAATTG GAAGTAGAAA TAATCCGAGC TCGTGGCCTT 
GTTGTAAAAC CAGGTTCAAA GACACTGCCA GCACCATATG TAAAGGTGTA TCTATTAGAA 
AATGGAGTCT GCATAGCCAA AAAGAAAACA AAGGTAGCAA GAAAAACGCT GGAACCGCTT 
TATCAGCAAC TTCTGTCATT TGAAGAAAGT CCTCAAGGCA AAGTTTTACA GATAATCGTT 
TGGGGAGACT ATGGACGTAT GGATCACAAG TCCTTTATGG GAGTGGCACA GATACTTTTA 
GATGAACTGG ACCTGTCCAA CATGGTGATT GGGTGGTTCA AACTCTTCCC TCCTTCTTCC 
CTAGTAGACC CAACTTTAGC TCCTCTCACT AGAAGAGCTT CCCAATCATC TCTTGAAAGT 
TCAACAGGAC CTTCGTATGC TCGCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014821001.1
CDS	43..4812
Translation	MPDLSHLTEEERKIILAVMDRQKKEEEKEQSVLKKLHQQFEMYKEQVKKMGEESQQQQEQKGDAPTCGICHKTKFADGCGHNCSYCQTKFCARCGGRVSLRSNKEDKVVMWVCNLCRKQQEILTKSGAWFYNSGSNAPQQPDQEGTRGLRNEEAPQEKKAKLQEHSQYQGPSGDVSTQVLDKNRSQGLTRQDSIKNGSGVKHQVTSDTTSDRKRSPSISREQNRRYDQRDERDEYSQYATSDSAMPRSPSDYSDRRSQRGPQLYEEPELGDYRDSNRRSRRRSKEYPVEEEDVQNREEYERQRREEEYQARYRSDPNLARYPVKPQPYEEQMRIHAEVSRARHERRHSDVSLANTELEDSRMSMLRMERPSRQRSVSERRAAMENQRSYSMERTREAQGPSPNRQRTTNHSPPTPRRSPIPLERPDMRRSDSLRKQHHLDPNSAVRKTKREKMETMLRNDSLSSDQSESVRPPPPKPHKTKKGGKMRQVSLSSSEEELASTPEYTSCDDVEIESESVSEKGDSQRGKRKTSEQAVLSDSNTLSQRQKKMVCFGGHSLEEDLEWSEPQIKDSGVDTCSSTTLNEEHSHSEKHPVTWQPSKDGDRLIGRILLNKRLKDGSVPRDSGAMLGLKVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGATFEEVYNIILESKPEPQVELVVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRPSISVTSPMSPGMLRDVPQFLSGQLSSQSLSRRTTPFVPRVQIKLWYDKVGHQLIVTILGAKDLPSREDGRPRNPYVKIYFLPDRSDKNKRRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARVREEESEFLGEILIELETALLDDEPHWYKLQTHDVSSLPLPHPSPYLPRRQLHGESPTRRLQNKGLYSYNSGSKRISDSEVSDYDCEDGIGVVSDYRHNGRDLQSSTLSVPEQVMSSNHCSRSGSPHRGDSIGRTRSWSPSVPPPQSRNVDQGPRGTRSTPAHYNTLNRMDRHRVIDDHYSPDRERNCEAADRQPYQRSRSTEQRPMLERTNSRSRSTERPDSNIIRSMPSLMTGRSAPPSPALPRSHPRTGSVQTSPSSTPVVGRRGRQLPQLPPKGTLERKEAESSRRRNSEECPEPFTTFEEKKADPENGDTGAMDVEERNRQMKMSKYKQVAGSDSRLEQDYHSKYRSGRDPQRGSDNVSNKSSDSDVSDVSAVSRTSSASRFSSTSYMSVQSERPRGNKKISVFTSKMQSRQMGASGKNMTKSTSIGGDMYTLEKNDGSQSDTAVGTVGGGGKKRRSSIGAKMVAIVGLSRKSRSTSQLSQTEAGGKKLRSTVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLAADSQFSDFLDGLGPAQLVGRQTLATPSMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLENGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELDLSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYARS

Target ORF information:

RefSeq Version	XM_014965515.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014965515.1

ORF Insert Sequence:

ATGCCCGACC TCAGCCACCT CACGGAGGAA GAGCGGAAGA TCATTCTGGC CGTCATGGAC 
CGCCAGAAGA AGGAAGAGGA GAAGGAGCAA TCCGTGCTCA AAAAATTACA TCAGCAGTTT 
GAAATGTATA AGGAGCAAGT GAAGAAGATG GGTGAAGAGT CACAACAGCA GCAGGAGCAG 
AAGGGTGATG CCCCAACGTG TGGCATCTGC CACAAAACAA AATTTGCTGA TGGCTGTGGC 
CATAACTGTT CATATTGCCA AACTAAATTC TGTGCACGCT GTGGAGGAAG AGTGTCTTTA 
CGGTCAAATA AGGAGGACAA AGTGGTAATG TGGGTATGTA ACTTGTGCCG AAAACAACAA 
GAAATCCTCA CAAAATCAGG AGCTTGGTTC TATAACAGTG GATCAAATGC ACCACAGCAG 
CCTGACCAAG AAGGTACTAG AGGTCTGCGG AACGAGGAAG CACCTCAAGA GAAAAAAGCA 
AAGCTGCAGG AGCATTCCCA GTACCAAGGA CCATCAGGTG ATGTATCCAC ACAAGTTTTG 
GACAAAAACA GATCTCAAGG GCTCACAAGA CAAGACTCAA TTAAGAATGG GTCAGGAGTA 
AAGCATCAAG TAACAAGTGA CACAACATCA GACAGGAAAA GAAGCCCATC AATATCAAGA 
GAACAGAACA GAAGATACGA CCAAAGGGAC GAGAGAGACG AATACTCACA GTATGCTACA 
TCAGACAGTG CGATGCCCAG GTCACCATCA GATTATTCCG ATAGGCGGTC GCAGCGTGGG 
CCTCAGTTAT ATGAAGAGCC TGAACTGGGT GATTATAGAG ATTCCAACAG AAGGAGTCGC 
AGGCGTTCCA AGGAGTATCC GGTAGAAGAG GAAGATGTAC AAAACAGGGA AGAATATGAA 
AGGCAGCGGA GGGAAGAGGA ATACCAGGCA CGGTACCGAA GCGATCCAAA TTTGGCTCGT 
TATCCAGTCA AGCCGCAACC GTATGAGGAA CAAATGCGTA TCCATGCTGA AGTGTCCCGG 
GCACGTCATG AACGGAGACA TAGTGATGTC TCATTGGCAA ATACTGAGTT GGAAGATTCT 
CGGATGTCTA TGCTGAGGAT GGAACGACCA TCAAGACAAA GATCAGTGTC TGAGCGTAGG 
GCTGCCATGG AAAATCAGCG CTCATACTCT ATGGAAAGAA CTCGAGAGGC TCAGGGACCA 
AGTCCCAATC GCCAGAGGAC CACAAATCAT AGTCCTCCTA CACCTAGGAG GAGTCCAATT 
CCTCTGGAAA GACCAGACAT GAGGCGCTCT GATTCATTAA GGAAACAACA CCATTTGGAT 
CCCAATTCTG CTGTACGGAA AACAAAACGT GAAAAGATGG AGACCATGTT ACGGAATGAT 
TCGCTCAGCT CAGACCAGTC TGAATCTGTC AGACCTCCAC CACCAAAGCC CCATAAAACG 
AAAAAGGGAG GTAAAATGCG CCAGGTTTCA TTAAGTAGTT CAGAAGAAGA ATTGGCGTCC 
ACTCCAGAGT ATACAAGTTG TGATGATGTA GAGATTGAAA GTGAAAGTGT TAGTGAAAAA 
GGGGACAGTC AAAGGGGAAA AAGAAAAACT AGTGAGCAGG CAGTTTTGTC GGATTCTAAC 
ACCTTATCCC AGAGGCAAAA GAAAATGGTG TGCTTTGGTG GCCACTCTTT GGAAGAGGAC 
TTGGAATGGT CTGAGCCTCA GATAAAAGAC TCTGGGGTAG ATACGTGTAG TAGCACAACT 
CTAAACGAGG AACATAGCCA TAGCGAGAAG CATCCAGTAA CTTGGCAACC ATCCAAAGAT 
GGAGATCGTC TCATTGGTCG GATTTTGTTA AATAAACGCC TAAAAGATGG AAGTGTGCCT 
AGAGACTCAG GAGCAATGCT TGGATTGAAG GTAGTAGGAG GAAAGATGAC TGAATCAGGT 
CGACTCTGCG CATTTATCAC CAAAGTTAAA AAAGGAAGCT TAGCCGATAC AGTGGGGCAT 
CTCAGACCAG GTGATGAAGT ATTAGAATGG AATGGAAGGC TACTACAAGG AGCCACGTTT 
GAGGAAGTGT ATAACATCAT TTTAGAATCC AAACCTGAGC CACAAGTGGA GCTTGTTGTT 
TCAAGACCAA TAGGGGACAT TCCCAGAATA CCTGACAGCA CACATGCACA GCTGGAGTCC 
AGCTCTAGTT CATTTGAATC TCAAAAAATG GACCGACCTT CTATTTCAGT GACTTCTCCT 
ATGAGTCCTG GCATGTTAAG GGATGTTCCA CAGTTCTTGT CTGGACAACT TTCAAGCCAA 
AGCCTTAGTA GAAGAACAAC GCCTTTTGTT CCTAGGGTTC AGATAAAACT GTGGTATGAC 
AAGGTTGGTC ATCAGTTAAT AGTGACGATA TTGGGAGCAA AGGATCTTCC TTCAAGGGAA 
GATGGGAGGC CAAGGAATCC TTATGTTAAA ATTTACTTTC TTCCAGACAG AAGTGATAAA 
AATAAAAGAA GAACAAAAAC AGTAAAGAAA ACATTGGAAC CTAAGTGGAA TCAGACATTC 
ATTTATTCTC CAGTTCATCG GAGAGAGTTT AGAGAACGAA TGCTAGAAAT TACTCTTTGG 
GACCAAGCAC GAGTTCGAGA GGAAGAAAGT GAATTTTTAG GAGAGATTCT TATTGAGTTA 
GAGACAGCTT TACTAGATGA CGAACCTCAC TGGTACAAAC TTCAAACGCA TGATGTCTCT 
TCATTGCCAC TTCCCCATCC CTCACCATAT TTGCCACGCA GACAGCTCCA TGGTGAGAGT 
CCCACACGGA GGTTACAAAA TAAAGGCTTA TATTCATATA ATTCAGGGTC CAAAAGAATC 
AGCGACAGTG AAGTCTCTGA TTATGACTGT GAAGATGGAA TTGGTGTTGT TTCAGATTAT 
CGACATAATG GGAGAGATCT TCAAAGTTCA ACGCTATCGG TGCCAGAACA AGTTATGTCA 
TCTAATCATT GCTCTCGATC AGGGTCCCCT CACCGTGGAG ATAGTATAGG ACGGACTAGA 
TCGTGGTCTC CCAGTGTCCC TCCCCCACAA AGTCGGAATG TGGATCAGGG ACCACGAGGG 
ACTCGATCTA CTCCTGCTCA TTACAATACC CTGAACCGAA TGGATAGACA CCGTGTCATA 
GATGACCACT ACTCCCCGGA CAGAGAGAGG AATTGTGAAG CAGCAGATAG ACAGCCATAT 
CAAAGATCCA GATCAACAGA GCAACGTCCT ATGCTAGAGC GGACCAACTC CCGCTCCCGA 
TCCACAGAGC GTCCTGACTC AAACATCATC AGGTCGATGC CTTCATTAAT GACTGGAAGA 
TCTGCCCCTC CTTCACCTGC CTTACCGAGG TCACATCCTC GAACTGGGTC TGTTCAGACA 
AGTCCATCAA GTACTCCAGT AGTAGGGCGA CGGGGCAGGC AGTTGCCGCA GCTGCCACCA 
AAGGGGACAT TGGAGAGAAA AGAAGCGGAA TCATCAAGGA GAAGAAACTC AGAGGAATGC 
CCTGAGCCAT TTACAACCTT TGAAGAAAAG AAAGCAGATC CAGAGAATGG GGATACAGGT 
GCAATGGATG TAGAAGAGAG GAATCGCCAA ATGAAAATGA GCAAGTACAA ACAGGTAGCA 
GGATCAGATT CCAGGCTGGA ACAAGATTAT CATTCTAAGT ATCGGTCAGG ACGGGATCCT 
CAGCGAGGCT CAGACAACGT TTCCAATAAG TCTTCAGACA GCGACGTCAG TGACGTCTCC 
GCCGTGTCAC GGACAAGCAG CGCTTCACGT TTCAGCAGCA CAAGCTACAT GTCTGTCCAG 
TCGGAGCGGC CAAGGGGAAA CAAAAAAATA AGTGTCTTTA CATCCAAAAT GCAAAGCAGA 
CAGATGGGCG CCTCAGGAAA GAACATGACT AAGAGCACCA GCATCGGCGG GGACATGTAC 
ACGCTGGAGA AGAACGACGG CAGCCAGTCA GACACCGCAG TGGGCACTGT TGGTGGTGGT 
GGCAAAAAGA GACGCTCCAG CATCGGTGCA AAGATGGTCG CCATTGTGGG TCTCTCGCGA 
AAAAGCCGTA GCACGTCACA ACTCAGCCAA ACTGAAGCAG GGGGCAAGAA GCTGCGGAGC 
ACCGTCCAGA GGAGCACTGA GACAGGGCTG GCCGTGGAGA TGAGGAACTG GATGACCCGT 
CAGGCCAGCC GGGAGTCAAC GGATGGGAGC ATGAACAGCT ACAGCTCTGA GGGAAATTTG 
ATTTTCCCTG GTGTGAGGTT GGCTGCAGAC AGCCAGTTCA GTGATTTTCT GGATGGACTT 
GGTCCTGCCC AGCTTGTAGG GCGACAGACT TTGGCGACAC CATCGATGGG AGATATTCAG 
GTGGGAATGA TGGACAAGAA AGGACAATTG GAAGTAGAAA TAATCCGAGC TCGTGGCCTT 
GTTGTAAAAC CAGGTTCAAA GACACTGCCA GCACCATATG TAAAGGTGTA TCTATTAGAA 
AATGGAGTCT GCATAGCCAA AAAGAAAACA AAGGTAGCAA GAAAAACGCT GGAACCGCTT 
TATCAGCAAC TTCTGTCATT TGAAGAAAGT CCTCAAGGCA AAGTTTTACA GATAATCGTT 
TGGGGAGACT ATGGACGTAT GGATCACAAG TCCTTTATGG GAGTGGCACA GATACTTTTA 
GATGAACTGG ACCTGTCCAA CATGGTGATT GGGTGGTTCA AACTCTTCCC TCCTTCTTCC 
CTAGTAGACC CAACTTTAGC TCCTCTCACT AGAAGAGCTT CCCAATCATC TCTTGAAAGT 
TCAACAGGAC CTTCGTATGC TCGCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIMS2 ( XM_014965521.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821007.1 Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X7, mRNA.

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CloneID	OCa81974
Clone ID Related Accession (Same CDS sequence)	XM_014965521.1
Accession Version	XM_014965521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	regulating synaptic membrane exocytosis protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGACC TCAGCCACCT CACGGAGGAA GAGCGGAAGA TCATTCTGGC CGTCATGGAC 
CGCCAGAAGA AGGAAGAGGA GAAGGAGCAA TCCGTGCTCA AGGTCAAAGA AGAGCAAAAA 
CCACAGCCGA CACAGTGGTT TCCCTTTAGT GGGATCACTG AGCTGGTAAA TAACGTTCTT 
CAACCACAGC AAAAACAGCA AAATGAAAAG GAGCCCCAGA CAAAATTACA TCAGCAGTTT 
GAAATGTATA AGGAGCAAGT GAAGAAGATG GGTGAAGAGT CACAACAGCA GCAGGAGCAG 
AAGGGTGATG CCCCAACGTG TGGCATCTGC CACAAAACAA AATTTGCTGA TGGCTGTGGC 
CATAACTGTT CATATTGCCA AACTAAATTC TGTGCACGCT GTGGAGGAAG AGTGTCTTTA 
CGGTCAAATA AGGTAATGTG GGTATGTAAC TTGTGCCGAA AACAACAAGA AATCCTCACA 
AAATCAGGAG CTTGGTTCTA TAACAGTGGA TCAAATGCAC CACAGCAGCC TGACCAAGAA 
GGTACTAGAG GTCTGCGGAA CGAGGAAGCA CCTCAAGAGA AAAAAGCAAA GCTGCAGGAG 
CATTCCCAGT ACCAAGGACC ATCAGGTGAT GTATCCACAC AAGTTTTGGA CAAAAACAGA 
TCTCAAGGGC TCACAAGACA AGACTCAATT AAGAATGGGT CAGGAGTAAA GCATCAAGTA 
ACAAGTGACA CAACATCAGA CAGGAAAAGA AGCCCATCAA TATCAAGAGA ACAGAACAGA 
AGATACGACC AAAGGGACGA GAGAGACGAA TACTCACAGT ATGCTACATC AGACAGTGCG 
ATGCCCAGGT CACCATCAGA TTATTCCGAT AGGCGGTCGC AGCGTGGGCC TCAGTTATAT 
GAAGAGCCTG AACTGGGTGA TTATAGAGAT TCCAACAGAA GGAGTCGCAG GCGTTCCAAG 
GAGTATCCGG TAGAAGAGGA AGATGTACAA AACAGGGAAG AATATGAAAG GCAGCGGAGG 
GAAGAGGAAT ACCAGGCACG GTACCGAAGC GATCCAAATT TGGCTCGTTA TCCAGTCAAG 
CCGCAACCGT ATGAGGAACA AATGCGTATC CATGCTGAAG TGTCCCGGGC ACGTCATGAA 
CGGAGACATA GTGATGTCTC ATTGGCAAAT ACTGAGTTGG AAGATTCTCG GATGTCTATG 
CTGAGGATGG AACGACCATC AAGACAAAGA TCAGTGTCTG AGCGTAGGGC TGCCATGGAA 
AATCAGCGCT CATACTCTAT GGAAAGAACT CGAGAGGCTC AGGGACCAAG TCCCAATCGC 
CAGAGGACCA CAAATCATAG TCCTCCTACA CCTAGGAGGA GTCCAATTCC TCTGGAAAGA 
CCAGACATGA GGCGCTCTGA TTCATTAAGG AAACAACACC ATTTGGATCC CAATTCTGCT 
GTACGGAAAA CAAAACGTGA AAAGATGGAG ACCATGTTAC GGAATGATTC GCTCAGCTCA 
GACCAGTCTG AATCTGTCAG ACCTCCACCA CCAAAGCCCC ATAAAACGAA AAAGGGAGGT 
AAAATGCGCC AGGTTTCATT AAGTAGTTCA GAAGAAGAAT TGGCGTCCAC TCCAGAGTAT 
ACAAGTTGTG ATGATGTAGA GATTGAAAGT GAAAGTGTTA GTGAAAAAGG AGACATGGAT 
TACAACTGGT TGGACCATAC GTCTTGGCAT AGCAGTGAGG CATCCCCAAT GTCTTTGCAT 
CCAGTAACTT GGCAACCATC CAAAGATGGA GATCGTCTCA TTGGTCGGAT TTTGTTAAAT 
AAACGCCTAA AAGATGGAAG TGTGCCTAGA GACTCAGGAG CAATGCTTGG ATTGAAGGTA 
GTAGGAGGAA AGATGACTGA ATCAGGTCGA CTCTGCGCAT TTATCACCAA AGTTAAAAAA 
GGAAGCTTAG CCGATACAGT GGGGCATCTC AGACCAGGTG ATGAAGTATT AGAATGGAAT 
GGAAGGCTAC TACAAGGAGC CACGTTTGAG GAAGTGTATA ACATCATTTT AGAATCCAAA 
CCTGAGCCAC AAGTGGAGCT TGTTGTTTCA AGACCAATAG GGGACATTCC CAGAATACCT 
GACAGCACAC ATGCACAGCT GGAGTCCAGC TCTAGTTCAT TTGAATCTCA AAAAATGGAC 
CGACCTTCTA TTTCAGTGAC TTCTCCTATG AGTCCTGGCA TGTTAAGGGA TGTTCCACAG 
TTCTTGTCTG GACAACTTTC AAGCCAAAGC CTTAGTAGAA GAACAACGCC TTTTGTTCCT 
AGGGTTCAGA TAAAACTGTG GTATGACAAG GTTGGTCATC AGTTAATAGT GACGATATTG 
GGAGCAAAGG ATCTTCCTTC AAGGGAAGAT GGGAGGCCAA GGAATCCTTA TGTTAAAATT 
TACTTTCTTC CAGACAGAAG TGATAAAAAT AAAAGAAGAA CAAAAACAGT AAAGAAAACA 
TTGGAACCTA AGTGGAATCA GACATTCATT TATTCTCCAG TTCATCGGAG AGAGTTTAGA 
GAACGAATGC TAGAAATTAC TCTTTGGGAC CAAGCACGAG TTCGAGAGGA AGAAAGTGAA 
TTTTTAGGAG AGATTCTTAT TGAGTTAGAG ACAGCTTTAC TAGATGACGA ACCTCACTGG 
TACAAACTTC AAACGCATGA TGTCTCTTCA TTGCCACTTC CCCATCCCTC ACCATATTTG 
CCACGCAGAC AGCTCCATGG TGAGAGTCCC ACACGGAGGT TACAAAGGTC CAAAAGAATC 
AGCGACAGTG AAGTCTCTGA TTATGACTGT GAAGATGGAA TTGGTGTTGT TTCAGATTAT 
CGACATAATG GGAGAGATCT TCAAAGTTCA ACGCTATCGG TGCCAGAACA AGTTATGTCA 
TCTAATCATT GCTCTCGATC AGGGTCCCCT CACCGTGGAG ATAGTATAGG ACGGACTAGA 
TCGTGGTCTC CCAGTGTCCC TCCCCCACAA AGTCGGAATG TGGATCAGGG ACCACGAGGG 
ACTCGATCTA CTCCTGCTCA TTACAATACC CTGAACCGAA TGGATAGACA CCGTGTCATA 
GATGACCACT ACTCCCCGGA CAGAGAGAGT CATTATGTTA CTTTGCCTCG TTCCCGATAT 
ACCCAGTCCA CAGATCACCA TTACAGAGAT GCCAGCCAGG ATCATGCAAT GTATCCTCTA 
TTTTGTGAAG ATGCAATCAG ATTACTTCGC TCCCGTAAGA TGTGCCGTAC CTATTCTGAG 
GGTGCTTACT ATGATCTTGA GAGGAGGACT AGGCAGGAGA GAAGAGTGCC TAATTCATAT 
TATGACGACA CAACCTATGC TCCTGAAAGG TGGTACAATG GTGCAAGTTC ATGGGCAGAT 
CATGTAGTCA ATGGTTCAGC TGAAAATTAC GGGAATTGTG AAGCAGCAGA TAGACAGCCA 
TATCAAAGAT CCAGATCAAC AGAGCAACGT CCTATGCTAG AGCGGACCAA CTCCCGCTCC 
CGATCCACAG AGCGTCCTGA CTCAAACATC ATCAGGTCGA TGCCTTCATT AATGACTGGA 
AGATCTGCCC CTCCTTCACC TGCCTTACCG AGGTCACATC CTCGAACTGG GTCTGTTCAG 
ACAAGTCCAT CAAGTACTCC AGTAGTAGGG CGACGGGGCA GGCAGTTGCC GCAGCTGCCA 
CCAAAGGGGA CATTGGAGAG AAAAGAAAAG AAAGCAGATC CAGAGAATGG GGATACAGGT 
GCAATGGATG TAGAAGAGAG GAATCGCCAA ATGAAAATGA GCAAGTACAA ACAGGTAGCA 
GGATCAGATT CCAGGCTGGA ACAAGATTAT CATTCTAAGT ATCGGTCAGG ACGGGATCCT 
CAGCGAGGCT CAGACAACGT TTCCAATAAG TCTTCAGACA GCGACGTCAG TGACGTCTCC 
GCCGTGTCAC GGACAAGCAG CGCTTCACGT TTCAGCAGCA CAAGCTACAT GTCTGTCCAG 
TCGGAGCGGC CAAGGGGAAA CAAAAAAATA AGTGTCTTTA CATCCAAAAT GCAAAGCAGA 
CAGATGGGCG CCTCAGGAAA GAACATGACT AAGAGCACCA GCATCGGCGG GGACATGTAC 
ACGCTGGAGA AGAACGACGG CAGCCAGTCA GACACCGCAG TGGGCACTGT TGGTGGTGGT 
GGCAAAAAGA GACGCTCCAG CATCGGTGCA AAGATGGTCG CCATTGTGGG TCTCTCGCGA 
AAAAGCCGTA GCACGTCACA ACTCAGCCAA ACTGAAGCAG GGGGCAAGAA GCTGCGGAGC 
ACCGTCCAGA GGAGCACTGA GACAGGGCTG GCCGTGGAGA TGAGGAACTG GATGACCCGT 
CAGGCCAGCC GGGAGTCAAC GGATGGGAGC ATGAACAGCT ACAGCTCTGA GGGAAATTTG 
ATTTTCCCTG GTGTGAGGTT GGCTGCAGAC AGCCAGTTCA GTGATTTTCT GGATGGACTT 
GGTCCTGCCC AGCTTGTAGG GCGACAGACT TTGGCGACAC CATCGATGGG AGATATTCAG 
GTGGGAATGA TGGACAAGAA AGGACAATTG GAAGTAGAAA TAATCCGAGC TCGTGGCCTT 
GTTGTAAAAC CAGGTTCAAA GACACTGCCA GCACCATATG TAAAGGTGTA TCTATTAGAA 
AATGGAGTCT GCATAGCCAA AAAGAAAACA AAGGTAGCAA GAAAAACGCT GGAACCGCTT 
TATCAGCAAC TTCTGTCATT TGAAGAAAGT CCTCAAGGCA AAGTTTTACA GATAATCGTT 
TGGGGAGACT ATGGACGTAT GGATCACAAG TCCTTTATGG GAGTGGCACA GATACTTTTA 
GATGAACTGG ACCTGTCCAA CATGGTGATT GGGTGGTTCA AACTCTTCCC TCCTTCTTCC 
CTAGTAGACC CAACTTTAGC TCCTCTCACT AGAAGAGCTT CCCAATCATC TCTTGAAAGT 
TCAACAGGAC CTTCGTATGC TCGCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014821007.1
CDS	43..4992
Translation	MPDLSHLTEEERKIILAVMDRQKKEEEKEQSVLKVKEEQKPQPTQWFPFSGITELVNNVLQPQQKQQNEKEPQTKLHQQFEMYKEQVKKMGEESQQQQEQKGDAPTCGICHKTKFADGCGHNCSYCQTKFCARCGGRVSLRSNKVMWVCNLCRKQQEILTKSGAWFYNSGSNAPQQPDQEGTRGLRNEEAPQEKKAKLQEHSQYQGPSGDVSTQVLDKNRSQGLTRQDSIKNGSGVKHQVTSDTTSDRKRSPSISREQNRRYDQRDERDEYSQYATSDSAMPRSPSDYSDRRSQRGPQLYEEPELGDYRDSNRRSRRRSKEYPVEEEDVQNREEYERQRREEEYQARYRSDPNLARYPVKPQPYEEQMRIHAEVSRARHERRHSDVSLANTELEDSRMSMLRMERPSRQRSVSERRAAMENQRSYSMERTREAQGPSPNRQRTTNHSPPTPRRSPIPLERPDMRRSDSLRKQHHLDPNSAVRKTKREKMETMLRNDSLSSDQSESVRPPPPKPHKTKKGGKMRQVSLSSSEEELASTPEYTSCDDVEIESESVSEKGDMDYNWLDHTSWHSSEASPMSLHPVTWQPSKDGDRLIGRILLNKRLKDGSVPRDSGAMLGLKVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGATFEEVYNIILESKPEPQVELVVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRPSISVTSPMSPGMLRDVPQFLSGQLSSQSLSRRTTPFVPRVQIKLWYDKVGHQLIVTILGAKDLPSREDGRPRNPYVKIYFLPDRSDKNKRRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARVREEESEFLGEILIELETALLDDEPHWYKLQTHDVSSLPLPHPSPYLPRRQLHGESPTRRLQRSKRISDSEVSDYDCEDGIGVVSDYRHNGRDLQSSTLSVPEQVMSSNHCSRSGSPHRGDSIGRTRSWSPSVPPPQSRNVDQGPRGTRSTPAHYNTLNRMDRHRVIDDHYSPDRESHYVTLPRSRYTQSTDHHYRDASQDHAMYPLFCEDAIRLLRSRKMCRTYSEGAYYDLERRTRQERRVPNSYYDDTTYAPERWYNGASSWADHVVNGSAENYGNCEAADRQPYQRSRSTEQRPMLERTNSRSRSTERPDSNIIRSMPSLMTGRSAPPSPALPRSHPRTGSVQTSPSSTPVVGRRGRQLPQLPPKGTLERKEKKADPENGDTGAMDVEERNRQMKMSKYKQVAGSDSRLEQDYHSKYRSGRDPQRGSDNVSNKSSDSDVSDVSAVSRTSSASRFSSTSYMSVQSERPRGNKKISVFTSKMQSRQMGASGKNMTKSTSIGGDMYTLEKNDGSQSDTAVGTVGGGGKKRRSSIGAKMVAIVGLSRKSRSTSQLSQTEAGGKKLRSTVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLAADSQFSDFLDGLGPAQLVGRQTLATPSMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLENGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELDLSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYARS

Target ORF information:

RefSeq Version	XM_014965521.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax regulating synaptic membrane exocytosis 2 (RIMS2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014965521.1

ORF Insert Sequence:

ATGCCCGACC TCAGCCACCT CACGGAGGAA GAGCGGAAGA TCATTCTGGC CGTCATGGAC 
CGCCAGAAGA AGGAAGAGGA GAAGGAGCAA TCCGTGCTCA AGGTCAAAGA AGAGCAAAAA 
CCACAGCCGA CACAGTGGTT TCCCTTTAGT GGGATCACTG AGCTGGTAAA TAACGTTCTT 
CAACCACAGC AAAAACAGCA AAATGAAAAG GAGCCCCAGA CAAAATTACA TCAGCAGTTT 
GAAATGTATA AGGAGCAAGT GAAGAAGATG GGTGAAGAGT CACAACAGCA GCAGGAGCAG 
AAGGGTGATG CCCCAACGTG TGGCATCTGC CACAAAACAA AATTTGCTGA TGGCTGTGGC 
CATAACTGTT CATATTGCCA AACTAAATTC TGTGCACGCT GTGGAGGAAG AGTGTCTTTA 
CGGTCAAATA AGGTAATGTG GGTATGTAAC TTGTGCCGAA AACAACAAGA AATCCTCACA 
AAATCAGGAG CTTGGTTCTA TAACAGTGGA TCAAATGCAC CACAGCAGCC TGACCAAGAA 
GGTACTAGAG GTCTGCGGAA CGAGGAAGCA CCTCAAGAGA AAAAAGCAAA GCTGCAGGAG 
CATTCCCAGT ACCAAGGACC ATCAGGTGAT GTATCCACAC AAGTTTTGGA CAAAAACAGA 
TCTCAAGGGC TCACAAGACA AGACTCAATT AAGAATGGGT CAGGAGTAAA GCATCAAGTA 
ACAAGTGACA CAACATCAGA CAGGAAAAGA AGCCCATCAA TATCAAGAGA ACAGAACAGA 
AGATACGACC AAAGGGACGA GAGAGACGAA TACTCACAGT ATGCTACATC AGACAGTGCG 
ATGCCCAGGT CACCATCAGA TTATTCCGAT AGGCGGTCGC AGCGTGGGCC TCAGTTATAT 
GAAGAGCCTG AACTGGGTGA TTATAGAGAT TCCAACAGAA GGAGTCGCAG GCGTTCCAAG 
GAGTATCCGG TAGAAGAGGA AGATGTACAA AACAGGGAAG AATATGAAAG GCAGCGGAGG 
GAAGAGGAAT ACCAGGCACG GTACCGAAGC GATCCAAATT TGGCTCGTTA TCCAGTCAAG 
CCGCAACCGT ATGAGGAACA AATGCGTATC CATGCTGAAG TGTCCCGGGC ACGTCATGAA 
CGGAGACATA GTGATGTCTC ATTGGCAAAT ACTGAGTTGG AAGATTCTCG GATGTCTATG 
CTGAGGATGG AACGACCATC AAGACAAAGA TCAGTGTCTG AGCGTAGGGC TGCCATGGAA 
AATCAGCGCT CATACTCTAT GGAAAGAACT CGAGAGGCTC AGGGACCAAG TCCCAATCGC 
CAGAGGACCA CAAATCATAG TCCTCCTACA CCTAGGAGGA GTCCAATTCC TCTGGAAAGA 
CCAGACATGA GGCGCTCTGA TTCATTAAGG AAACAACACC ATTTGGATCC CAATTCTGCT 
GTACGGAAAA CAAAACGTGA AAAGATGGAG ACCATGTTAC GGAATGATTC GCTCAGCTCA 
GACCAGTCTG AATCTGTCAG ACCTCCACCA CCAAAGCCCC ATAAAACGAA AAAGGGAGGT 
AAAATGCGCC AGGTTTCATT AAGTAGTTCA GAAGAAGAAT TGGCGTCCAC TCCAGAGTAT 
ACAAGTTGTG ATGATGTAGA GATTGAAAGT GAAAGTGTTA GTGAAAAAGG AGACATGGAT 
TACAACTGGT TGGACCATAC GTCTTGGCAT AGCAGTGAGG CATCCCCAAT GTCTTTGCAT 
CCAGTAACTT GGCAACCATC CAAAGATGGA GATCGTCTCA TTGGTCGGAT TTTGTTAAAT 
AAACGCCTAA AAGATGGAAG TGTGCCTAGA GACTCAGGAG CAATGCTTGG ATTGAAGGTA 
GTAGGAGGAA AGATGACTGA ATCAGGTCGA CTCTGCGCAT TTATCACCAA AGTTAAAAAA 
GGAAGCTTAG CCGATACAGT GGGGCATCTC AGACCAGGTG ATGAAGTATT AGAATGGAAT 
GGAAGGCTAC TACAAGGAGC CACGTTTGAG GAAGTGTATA ACATCATTTT AGAATCCAAA 
CCTGAGCCAC AAGTGGAGCT TGTTGTTTCA AGACCAATAG GGGACATTCC CAGAATACCT 
GACAGCACAC ATGCACAGCT GGAGTCCAGC TCTAGTTCAT TTGAATCTCA AAAAATGGAC 
CGACCTTCTA TTTCAGTGAC TTCTCCTATG AGTCCTGGCA TGTTAAGGGA TGTTCCACAG 
TTCTTGTCTG GACAACTTTC AAGCCAAAGC CTTAGTAGAA GAACAACGCC TTTTGTTCCT 
AGGGTTCAGA TAAAACTGTG GTATGACAAG GTTGGTCATC AGTTAATAGT GACGATATTG 
GGAGCAAAGG ATCTTCCTTC AAGGGAAGAT GGGAGGCCAA GGAATCCTTA TGTTAAAATT 
TACTTTCTTC CAGACAGAAG TGATAAAAAT AAAAGAAGAA CAAAAACAGT AAAGAAAACA 
TTGGAACCTA AGTGGAATCA GACATTCATT TATTCTCCAG TTCATCGGAG AGAGTTTAGA 
GAACGAATGC TAGAAATTAC TCTTTGGGAC CAAGCACGAG TTCGAGAGGA AGAAAGTGAA 
TTTTTAGGAG AGATTCTTAT TGAGTTAGAG ACAGCTTTAC TAGATGACGA ACCTCACTGG 
TACAAACTTC AAACGCATGA TGTCTCTTCA TTGCCACTTC CCCATCCCTC ACCATATTTG 
CCACGCAGAC AGCTCCATGG TGAGAGTCCC ACACGGAGGT TACAAAGGTC CAAAAGAATC 
AGCGACAGTG AAGTCTCTGA TTATGACTGT GAAGATGGAA TTGGTGTTGT TTCAGATTAT 
CGACATAATG GGAGAGATCT TCAAAGTTCA ACGCTATCGG TGCCAGAACA AGTTATGTCA 
TCTAATCATT GCTCTCGATC AGGGTCCCCT CACCGTGGAG ATAGTATAGG ACGGACTAGA 
TCGTGGTCTC CCAGTGTCCC TCCCCCACAA AGTCGGAATG TGGATCAGGG ACCACGAGGG 
ACTCGATCTA CTCCTGCTCA TTACAATACC CTGAACCGAA TGGATAGACA CCGTGTCATA 
GATGACCACT ACTCCCCGGA CAGAGAGAGT CATTATGTTA CTTTGCCTCG TTCCCGATAT 
ACCCAGTCCA CAGATCACCA TTACAGAGAT GCCAGCCAGG ATCATGCAAT GTATCCTCTA 
TTTTGTGAAG ATGCAATCAG ATTACTTCGC TCCCGTAAGA TGTGCCGTAC CTATTCTGAG 
GGTGCTTACT ATGATCTTGA GAGGAGGACT AGGCAGGAGA GAAGAGTGCC TAATTCATAT 
TATGACGACA CAACCTATGC TCCTGAAAGG TGGTACAATG GTGCAAGTTC ATGGGCAGAT 
CATGTAGTCA ATGGTTCAGC TGAAAATTAC GGGAATTGTG AAGCAGCAGA TAGACAGCCA 
TATCAAAGAT CCAGATCAAC AGAGCAACGT CCTATGCTAG AGCGGACCAA CTCCCGCTCC 
CGATCCACAG AGCGTCCTGA CTCAAACATC ATCAGGTCGA TGCCTTCATT AATGACTGGA 
AGATCTGCCC CTCCTTCACC TGCCTTACCG AGGTCACATC CTCGAACTGG GTCTGTTCAG 
ACAAGTCCAT CAAGTACTCC AGTAGTAGGG CGACGGGGCA GGCAGTTGCC GCAGCTGCCA 
CCAAAGGGGA CATTGGAGAG AAAAGAAAAG AAAGCAGATC CAGAGAATGG GGATACAGGT 
GCAATGGATG TAGAAGAGAG GAATCGCCAA ATGAAAATGA GCAAGTACAA ACAGGTAGCA 
GGATCAGATT CCAGGCTGGA ACAAGATTAT CATTCTAAGT ATCGGTCAGG ACGGGATCCT 
CAGCGAGGCT CAGACAACGT TTCCAATAAG TCTTCAGACA GCGACGTCAG TGACGTCTCC 
GCCGTGTCAC GGACAAGCAG CGCTTCACGT TTCAGCAGCA CAAGCTACAT GTCTGTCCAG 
TCGGAGCGGC CAAGGGGAAA CAAAAAAATA AGTGTCTTTA CATCCAAAAT GCAAAGCAGA 
CAGATGGGCG CCTCAGGAAA GAACATGACT AAGAGCACCA GCATCGGCGG GGACATGTAC 
ACGCTGGAGA AGAACGACGG CAGCCAGTCA GACACCGCAG TGGGCACTGT TGGTGGTGGT 
GGCAAAAAGA GACGCTCCAG CATCGGTGCA AAGATGGTCG CCATTGTGGG TCTCTCGCGA 
AAAAGCCGTA GCACGTCACA ACTCAGCCAA ACTGAAGCAG GGGGCAAGAA GCTGCGGAGC 
ACCGTCCAGA GGAGCACTGA GACAGGGCTG GCCGTGGAGA TGAGGAACTG GATGACCCGT 
CAGGCCAGCC GGGAGTCAAC GGATGGGAGC ATGAACAGCT ACAGCTCTGA GGGAAATTTG 
ATTTTCCCTG GTGTGAGGTT GGCTGCAGAC AGCCAGTTCA GTGATTTTCT GGATGGACTT 
GGTCCTGCCC AGCTTGTAGG GCGACAGACT TTGGCGACAC CATCGATGGG AGATATTCAG 
GTGGGAATGA TGGACAAGAA AGGACAATTG GAAGTAGAAA TAATCCGAGC TCGTGGCCTT 
GTTGTAAAAC CAGGTTCAAA GACACTGCCA GCACCATATG TAAAGGTGTA TCTATTAGAA 
AATGGAGTCT GCATAGCCAA AAAGAAAACA AAGGTAGCAA GAAAAACGCT GGAACCGCTT 
TATCAGCAAC TTCTGTCATT TGAAGAAAGT CCTCAAGGCA AAGTTTTACA GATAATCGTT 
TGGGGAGACT ATGGACGTAT GGATCACAAG TCCTTTATGG GAGTGGCACA GATACTTTTA 
GATGAACTGG ACCTGTCCAA CATGGTGATT GGGTGGTTCA AACTCTTCCC TCCTTCTTCC 
CTAGTAGACC CAACTTTAGC TCCTCTCACT AGAAGAGCTT CCCAATCATC TCTTGAAAGT 
TCAACAGGAC CTTCGTATGC TCGCTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RIMS2 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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