ORF » Species Summary » Calidris pugnax » TMEM74 cDNA ORF clone

TMEM74 cDNA ORF clone, Calidris pugnax(ruff)

The following TMEM74 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM74 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa82073 XM_014965659.1
Latest version!		 Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OCa82073 XM_014965662.1
Latest version!		 Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OCa82073 XM_014965661.1
Latest version!		 Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OCa82073 XM_014965660.1
Latest version!		 Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    TMEM74 ( XM_014965660.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821146.1 Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCa82073
    Clone ID Related Accession (Same CDS sequence) XM_014965660.1 , XM_014965661.1 , XM_014965662.1 , XM_014965659.1
    Accession Version XM_014965660.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane protein 74
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTTGCA TGGAGCTTCT CTACCTGGCG GAGGAGAGCA GGCAGGCGCC CCTGGGCACC 
    GCTGCCGGCT GGAGCCTGCC CTCCCCTCCC TACGATCAGC AGCAGCAGCA GCAGCGTGAG 
    GGGGGTGAGG TGGACCCCAG AGCAGCCGCC GCCGTGTCAG CCCTGCGCTG CGAAAGGCAC 
    TGCAAGCCCT TGCAGAGGGG CCCAGTGGCC GAGCGCCCCC CGGCACCCCA GCCTGCCCCC 
    CGGGACACCT CTCCCCAGCA GCACCCCGCG CCCCACGGCT CCTCCCAGCC CTGCCAGCCG 
    CCCGAGCGCT TGCCTGGGGA AGAGGAGGAA GGGAAGAAGA AAGCCTGCTG CTGTGCCCAG 
    GAGCTGGAGA CATCATTCAC CTACGTGGAT GAAAATGTAA ACCTGGAGCA TGCAAGAAGT 
    CCCCCCAGTC CTACAGGCGG CCACTGCCAG GATGCCCCTC CGCAGCAGGG TTCCTGCAGG 
    GAGCTGCCAC CCGAATGGGT GCACGATTCC CCTTCCCTGG TCTCCGAGGA GGACGACGCT 
    GCCTCGGAGG CAGCGGCAGG GAAATCCGTG GACTACGGGT TCATTAGTGC CATTTTGTTC 
    CTGGTTAGTG GCATTTTGCT GGTGATCATT TCCTACGTGG TACCCAGAGA CGTGACTGTG 
    GATCCCAACA CGGTGGCTGC TCGGGAGATG GAGAGGCTGG AGAACGAGAG CGCAAGGATC 
    GGGGCTCACT TGGACCGCTG CGTTATCGCC GGGCTGTGTC TCTTAACCCT GGGGGGAGTG 
    GTGCTCTCCA GCCTGCTGAT GATGTCCATG TGGAAAGGGG AGCTGTACCG GAGGAGCAGG 
    TTTGCGTCCT CCAAGGAGTC TGCCAAGCTC TATGGGTCTT TCAATTTCAG AATGAAGTCT 
    GGTGCGAATG ATAATATGCT CGAGCTGTCG TTAGTCGAGG AAGATGTGCT TGCCATAGAT 
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014821146.1
    CDS292..1257
    Translation

    Target ORF information:

    RefSeq Version XM_014965660.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014965660.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTTGCA TGGAGCTTCT CTACCTGGCG GAGGAGAGCA GGCAGGCGCC CCTGGGCACC 
    GCTGCCGGCT GGAGCCTGCC CTCCCCTCCC TACGATCAGC AGCAGCAGCA GCAGCGTGAG 
    GGGGGTGAGG TGGACCCCAG AGCAGCCGCC GCCGTGTCAG CCCTGCGCTG CGAAAGGCAC 
    TGCAAGCCCT TGCAGAGGGG CCCAGTGGCC GAGCGCCCCC CGGCACCCCA GCCTGCCCCC 
    CGGGACACCT CTCCCCAGCA GCACCCCGCG CCCCACGGCT CCTCCCAGCC CTGCCAGCCG 
    CCCGAGCGCT TGCCTGGGGA AGAGGAGGAA GGGAAGAAGA AAGCCTGCTG CTGTGCCCAG 
    GAGCTGGAGA CATCATTCAC CTACGTGGAT GAAAATGTAA ACCTGGAGCA TGCAAGAAGT 
    CCCCCCAGTC CTACAGGCGG CCACTGCCAG GATGCCCCTC CGCAGCAGGG TTCCTGCAGG 
    GAGCTGCCAC CCGAATGGGT GCACGATTCC CCTTCCCTGG TCTCCGAGGA GGACGACGCT 
    GCCTCGGAGG CAGCGGCAGG GAAATCCGTG GACTACGGGT TCATTAGTGC CATTTTGTTC 
    CTGGTTAGTG GCATTTTGCT GGTGATCATT TCCTACGTGG TACCCAGAGA CGTGACTGTG 
    GATCCCAACA CGGTGGCTGC TCGGGAGATG GAGAGGCTGG AGAACGAGAG CGCAAGGATC 
    GGGGCTCACT TGGACCGCTG CGTTATCGCC GGGCTGTGTC TCTTAACCCT GGGGGGAGTG 
    GTGCTCTCCA GCCTGCTGAT GATGTCCATG TGGAAAGGGG AGCTGTACCG GAGGAGCAGG 
    TTTGCGTCCT CCAAGGAGTC TGCCAAGCTC TATGGGTCTT TCAATTTCAG AATGAAGTCT 
    GGTGCGAATG ATAATATGCT CGAGCTGTCG TTAGTCGAGG AAGATGTGCT TGCCATAGAT 
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TMEM74 ( XM_014965661.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821147.1 Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OCa82073
    Clone ID Related Accession (Same CDS sequence) XM_014965660.1 , XM_014965661.1 , XM_014965662.1 , XM_014965659.1
    Accession Version XM_014965661.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane protein 74
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTTGCA TGGAGCTTCT CTACCTGGCG GAGGAGAGCA GGCAGGCGCC CCTGGGCACC 
    GCTGCCGGCT GGAGCCTGCC CTCCCCTCCC TACGATCAGC AGCAGCAGCA GCAGCGTGAG 
    GGGGGTGAGG TGGACCCCAG AGCAGCCGCC GCCGTGTCAG CCCTGCGCTG CGAAAGGCAC 
    TGCAAGCCCT TGCAGAGGGG CCCAGTGGCC GAGCGCCCCC CGGCACCCCA GCCTGCCCCC 
    CGGGACACCT CTCCCCAGCA GCACCCCGCG CCCCACGGCT CCTCCCAGCC CTGCCAGCCG 
    CCCGAGCGCT TGCCTGGGGA AGAGGAGGAA GGGAAGAAGA AAGCCTGCTG CTGTGCCCAG 
    GAGCTGGAGA CATCATTCAC CTACGTGGAT GAAAATGTAA ACCTGGAGCA TGCAAGAAGT 
    CCCCCCAGTC CTACAGGCGG CCACTGCCAG GATGCCCCTC CGCAGCAGGG TTCCTGCAGG 
    GAGCTGCCAC CCGAATGGGT GCACGATTCC CCTTCCCTGG TCTCCGAGGA GGACGACGCT 
    GCCTCGGAGG CAGCGGCAGG GAAATCCGTG GACTACGGGT TCATTAGTGC CATTTTGTTC 
    CTGGTTAGTG GCATTTTGCT GGTGATCATT TCCTACGTGG TACCCAGAGA CGTGACTGTG 
    GATCCCAACA CGGTGGCTGC TCGGGAGATG GAGAGGCTGG AGAACGAGAG CGCAAGGATC 
    GGGGCTCACT TGGACCGCTG CGTTATCGCC GGGCTGTGTC TCTTAACCCT GGGGGGAGTG 
    GTGCTCTCCA GCCTGCTGAT GATGTCCATG TGGAAAGGGG AGCTGTACCG GAGGAGCAGG 
    TTTGCGTCCT CCAAGGAGTC TGCCAAGCTC TATGGGTCTT TCAATTTCAG AATGAAGTCT 
    GGTGCGAATG ATAATATGCT CGAGCTGTCG TTAGTCGAGG AAGATGTGCT TGCCATAGAT 
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014821147.1
    CDS227..1192
    Translation

    Target ORF information:

    RefSeq Version XM_014965661.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014965661.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTTGCA TGGAGCTTCT CTACCTGGCG GAGGAGAGCA GGCAGGCGCC CCTGGGCACC 
    GCTGCCGGCT GGAGCCTGCC CTCCCCTCCC TACGATCAGC AGCAGCAGCA GCAGCGTGAG 
    GGGGGTGAGG TGGACCCCAG AGCAGCCGCC GCCGTGTCAG CCCTGCGCTG CGAAAGGCAC 
    TGCAAGCCCT TGCAGAGGGG CCCAGTGGCC GAGCGCCCCC CGGCACCCCA GCCTGCCCCC 
    CGGGACACCT CTCCCCAGCA GCACCCCGCG CCCCACGGCT CCTCCCAGCC CTGCCAGCCG 
    CCCGAGCGCT TGCCTGGGGA AGAGGAGGAA GGGAAGAAGA AAGCCTGCTG CTGTGCCCAG 
    GAGCTGGAGA CATCATTCAC CTACGTGGAT GAAAATGTAA ACCTGGAGCA TGCAAGAAGT 
    CCCCCCAGTC CTACAGGCGG CCACTGCCAG GATGCCCCTC CGCAGCAGGG TTCCTGCAGG 
    GAGCTGCCAC CCGAATGGGT GCACGATTCC CCTTCCCTGG TCTCCGAGGA GGACGACGCT 
    GCCTCGGAGG CAGCGGCAGG GAAATCCGTG GACTACGGGT TCATTAGTGC CATTTTGTTC 
    CTGGTTAGTG GCATTTTGCT GGTGATCATT TCCTACGTGG TACCCAGAGA CGTGACTGTG 
    GATCCCAACA CGGTGGCTGC TCGGGAGATG GAGAGGCTGG AGAACGAGAG CGCAAGGATC 
    GGGGCTCACT TGGACCGCTG CGTTATCGCC GGGCTGTGTC TCTTAACCCT GGGGGGAGTG 
    GTGCTCTCCA GCCTGCTGAT GATGTCCATG TGGAAAGGGG AGCTGTACCG GAGGAGCAGG 
    TTTGCGTCCT CCAAGGAGTC TGCCAAGCTC TATGGGTCTT TCAATTTCAG AATGAAGTCT 
    GGTGCGAATG ATAATATGCT CGAGCTGTCG TTAGTCGAGG AAGATGTGCT TGCCATAGAT 
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TMEM74 ( XM_014965662.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821148.1 Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OCa82073
    Clone ID Related Accession (Same CDS sequence) XM_014965660.1 , XM_014965661.1 , XM_014965662.1 , XM_014965659.1
    Accession Version XM_014965662.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane protein 74
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTTGCA TGGAGCTTCT CTACCTGGCG GAGGAGAGCA GGCAGGCGCC CCTGGGCACC 
    GCTGCCGGCT GGAGCCTGCC CTCCCCTCCC TACGATCAGC AGCAGCAGCA GCAGCGTGAG 
    GGGGGTGAGG TGGACCCCAG AGCAGCCGCC GCCGTGTCAG CCCTGCGCTG CGAAAGGCAC 
    TGCAAGCCCT TGCAGAGGGG CCCAGTGGCC GAGCGCCCCC CGGCACCCCA GCCTGCCCCC 
    CGGGACACCT CTCCCCAGCA GCACCCCGCG CCCCACGGCT CCTCCCAGCC CTGCCAGCCG 
    CCCGAGCGCT TGCCTGGGGA AGAGGAGGAA GGGAAGAAGA AAGCCTGCTG CTGTGCCCAG 
    GAGCTGGAGA CATCATTCAC CTACGTGGAT GAAAATGTAA ACCTGGAGCA TGCAAGAAGT 
    CCCCCCAGTC CTACAGGCGG CCACTGCCAG GATGCCCCTC CGCAGCAGGG TTCCTGCAGG 
    GAGCTGCCAC CCGAATGGGT GCACGATTCC CCTTCCCTGG TCTCCGAGGA GGACGACGCT 
    GCCTCGGAGG CAGCGGCAGG GAAATCCGTG GACTACGGGT TCATTAGTGC CATTTTGTTC 
    CTGGTTAGTG GCATTTTGCT GGTGATCATT TCCTACGTGG TACCCAGAGA CGTGACTGTG 
    GATCCCAACA CGGTGGCTGC TCGGGAGATG GAGAGGCTGG AGAACGAGAG CGCAAGGATC 
    GGGGCTCACT TGGACCGCTG CGTTATCGCC GGGCTGTGTC TCTTAACCCT GGGGGGAGTG 
    GTGCTCTCCA GCCTGCTGAT GATGTCCATG TGGAAAGGGG AGCTGTACCG GAGGAGCAGG 
    TTTGCGTCCT CCAAGGAGTC TGCCAAGCTC TATGGGTCTT TCAATTTCAG AATGAAGTCT 
    GGTGCGAATG ATAATATGCT CGAGCTGTCG TTAGTCGAGG AAGATGTGCT TGCCATAGAT 
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014821148.1
    CDS182..1147
    Translation

    Target ORF information:

    RefSeq Version XM_014965662.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014965662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTTGCA TGGAGCTTCT CTACCTGGCG GAGGAGAGCA GGCAGGCGCC CCTGGGCACC 
    GCTGCCGGCT GGAGCCTGCC CTCCCCTCCC TACGATCAGC AGCAGCAGCA GCAGCGTGAG 
    GGGGGTGAGG TGGACCCCAG AGCAGCCGCC GCCGTGTCAG CCCTGCGCTG CGAAAGGCAC 
    TGCAAGCCCT TGCAGAGGGG CCCAGTGGCC GAGCGCCCCC CGGCACCCCA GCCTGCCCCC 
    CGGGACACCT CTCCCCAGCA GCACCCCGCG CCCCACGGCT CCTCCCAGCC CTGCCAGCCG 
    CCCGAGCGCT TGCCTGGGGA AGAGGAGGAA GGGAAGAAGA AAGCCTGCTG CTGTGCCCAG 
    GAGCTGGAGA CATCATTCAC CTACGTGGAT GAAAATGTAA ACCTGGAGCA TGCAAGAAGT 
    CCCCCCAGTC CTACAGGCGG CCACTGCCAG GATGCCCCTC CGCAGCAGGG TTCCTGCAGG 
    GAGCTGCCAC CCGAATGGGT GCACGATTCC CCTTCCCTGG TCTCCGAGGA GGACGACGCT 
    GCCTCGGAGG CAGCGGCAGG GAAATCCGTG GACTACGGGT TCATTAGTGC CATTTTGTTC 
    CTGGTTAGTG GCATTTTGCT GGTGATCATT TCCTACGTGG TACCCAGAGA CGTGACTGTG 
    GATCCCAACA CGGTGGCTGC TCGGGAGATG GAGAGGCTGG AGAACGAGAG CGCAAGGATC 
    GGGGCTCACT TGGACCGCTG CGTTATCGCC GGGCTGTGTC TCTTAACCCT GGGGGGAGTG 
    GTGCTCTCCA GCCTGCTGAT GATGTCCATG TGGAAAGGGG AGCTGTACCG GAGGAGCAGG 
    TTTGCGTCCT CCAAGGAGTC TGCCAAGCTC TATGGGTCTT TCAATTTCAG AATGAAGTCT 
    GGTGCGAATG ATAATATGCT CGAGCTGTCG TTAGTCGAGG AAGATGTGCT TGCCATAGAT 
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TMEM74 ( XM_014965659.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014821145.1 Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCa82073
    Clone ID Related Accession (Same CDS sequence) XM_014965660.1 , XM_014965661.1 , XM_014965662.1 , XM_014965659.1
    Accession Version XM_014965659.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product transmembrane protein 74
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094509.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTTGCA TGGAGCTTCT CTACCTGGCG GAGGAGAGCA GGCAGGCGCC CCTGGGCACC 
    GCTGCCGGCT GGAGCCTGCC CTCCCCTCCC TACGATCAGC AGCAGCAGCA GCAGCGTGAG 
    GGGGGTGAGG TGGACCCCAG AGCAGCCGCC GCCGTGTCAG CCCTGCGCTG CGAAAGGCAC 
    TGCAAGCCCT TGCAGAGGGG CCCAGTGGCC GAGCGCCCCC CGGCACCCCA GCCTGCCCCC 
    CGGGACACCT CTCCCCAGCA GCACCCCGCG CCCCACGGCT CCTCCCAGCC CTGCCAGCCG 
    CCCGAGCGCT TGCCTGGGGA AGAGGAGGAA GGGAAGAAGA AAGCCTGCTG CTGTGCCCAG 
    GAGCTGGAGA CATCATTCAC CTACGTGGAT GAAAATGTAA ACCTGGAGCA TGCAAGAAGT 
    CCCCCCAGTC CTACAGGCGG CCACTGCCAG GATGCCCCTC CGCAGCAGGG TTCCTGCAGG 
    GAGCTGCCAC CCGAATGGGT GCACGATTCC CCTTCCCTGG TCTCCGAGGA GGACGACGCT 
    GCCTCGGAGG CAGCGGCAGG GAAATCCGTG GACTACGGGT TCATTAGTGC CATTTTGTTC 
    CTGGTTAGTG GCATTTTGCT GGTGATCATT TCCTACGTGG TACCCAGAGA CGTGACTGTG 
    GATCCCAACA CGGTGGCTGC TCGGGAGATG GAGAGGCTGG AGAACGAGAG CGCAAGGATC 
    GGGGCTCACT TGGACCGCTG CGTTATCGCC GGGCTGTGTC TCTTAACCCT GGGGGGAGTG 
    GTGCTCTCCA GCCTGCTGAT GATGTCCATG TGGAAAGGGG AGCTGTACCG GAGGAGCAGG 
    TTTGCGTCCT CCAAGGAGTC TGCCAAGCTC TATGGGTCTT TCAATTTCAG AATGAAGTCT 
    GGTGCGAATG ATAATATGCT CGAGCTGTCG TTAGTCGAGG AAGATGTGCT TGCCATAGAT 
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014821145.1
    CDS217..1182
    Translation

    Target ORF information:

    RefSeq Version XM_014965659.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax transmembrane protein 74 (TMEM74), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014965659.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTTGCA TGGAGCTTCT CTACCTGGCG GAGGAGAGCA GGCAGGCGCC CCTGGGCACC 
    GCTGCCGGCT GGAGCCTGCC CTCCCCTCCC TACGATCAGC AGCAGCAGCA GCAGCGTGAG 
    GGGGGTGAGG TGGACCCCAG AGCAGCCGCC GCCGTGTCAG CCCTGCGCTG CGAAAGGCAC 
    TGCAAGCCCT TGCAGAGGGG CCCAGTGGCC GAGCGCCCCC CGGCACCCCA GCCTGCCCCC 
    CGGGACACCT CTCCCCAGCA GCACCCCGCG CCCCACGGCT CCTCCCAGCC CTGCCAGCCG 
    CCCGAGCGCT TGCCTGGGGA AGAGGAGGAA GGGAAGAAGA AAGCCTGCTG CTGTGCCCAG 
    GAGCTGGAGA CATCATTCAC CTACGTGGAT GAAAATGTAA ACCTGGAGCA TGCAAGAAGT 
    CCCCCCAGTC CTACAGGCGG CCACTGCCAG GATGCCCCTC CGCAGCAGGG TTCCTGCAGG 
    GAGCTGCCAC CCGAATGGGT GCACGATTCC CCTTCCCTGG TCTCCGAGGA GGACGACGCT 
    GCCTCGGAGG CAGCGGCAGG GAAATCCGTG GACTACGGGT TCATTAGTGC CATTTTGTTC 
    CTGGTTAGTG GCATTTTGCT GGTGATCATT TCCTACGTGG TACCCAGAGA CGTGACTGTG 
    GATCCCAACA CGGTGGCTGC TCGGGAGATG GAGAGGCTGG AGAACGAGAG CGCAAGGATC 
    GGGGCTCACT TGGACCGCTG CGTTATCGCC GGGCTGTGTC TCTTAACCCT GGGGGGAGTG 
    GTGCTCTCCA GCCTGCTGAT GATGTCCATG TGGAAAGGGG AGCTGTACCG GAGGAGCAGG 
    TTTGCGTCCT CCAAGGAGTC TGCCAAGCTC TATGGGTCTT TCAATTTCAG AATGAAGTCT 
    GGTGCGAATG ATAATATGCT CGAGCTGTCG TTAGTCGAGG AAGATGTGCT TGCCATAGAT 
    AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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