CHRNA4 cDNA ORF clone, Canis lupus familiaris(Dog)

  • Gene

  • Clones

  • gRNAs

The following CHRNA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe25180 XM_005635288.2
Latest version!
Canis lupus familiaris cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe42930 XM_014107318.1
Latest version!
Canis lupus familiaris cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCe25179 XM_543097.5
Latest version!
Canis lupus familiaris cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe296851 XM_022409244.1
Latest version!
Canis lupus familiaris cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe25180
    Clone ID Related Accession (Same CDS sequence) XM_005635288.2
    Accession Version XM_005635288.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAGTTCG GGTCCTGGAC GTACGACAAG GCCAAGATAG ACCTGGTGAG CATGCACAGC 
    CGCGTGGACC AGCTGGACCT GTGGGAGAGC GGCGAGTGGG TCATCGTGGA CGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGC GCCGAGGTCT ACCCGGACAT CACCTACGCC
    TTCGTCATCC GGCGCCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT CGTCTTCTAC CTGCCGTCGG AGTGCGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT CTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCCTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTCTTCAC CATGATCTTC
    GTCACGCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCGCGC
    ACGCACACCA TGCCCGCCTG GGTCCGCCGG GTCTTCCTGG ACGTCGTGCC CCGCCTGCTC
    TTCATGAAGC GGCCGTCCGT GGTCAAGGAC AACTGCAGGC GGCTCATCGA ATCCATGCAC
    AAGATGGCCA GCGCCCCAGG CTTCTGGCCC GAGCCCGAGG GGGAGCCCGG CGTCCCGAGC
    GGAACCCAGA GCCGGGGCCC CTCGCGCACC CCGTCCTTCT GCGGCCCCGT GGACGAGCTG
    GCCAAGCCCC CGCCGGCCTG CAAGTCGCCC TCGGGCCAGG CCCCTGCTCT GCGGCCCTCA
    GAGGCGGAGG AAGCGAGCCC CCGCCCCTCG CCTGACTCCT GCCGTCCCCT CACCAAAGCC
    CGGTCCCTGA GTGTCCAGCA TGTGTCCAGT CCTCCCGAAG GGGCGGAGGA CGGCGTCCGC
    TGCCGGTCCC GGAGCATCCA GTACGGCGGT CCCCAGGACG CCGCCGCCTC TGAAGCTGGG
    GGCCCCGTGG CCGGCTCCCC GGCCTTGCTC AAGGCCAGCT CGGCCGAGCT CCCGGCCCCG
    GAGCAGCCCT CTCCCTGCAA ATGCAGGTGC AAGAAGGAGC CGTCCCCGAG CGCCGTGCCC
    AAAGCCCGGG GCACCAAAGC GCCCCCCCGG CACCTGCCCC TGTCTCCGGC CCTGACGCGG
    GCGGTGGAGG GCGTCCAGTA CATCGCGGAC CACCTGAAGG CGGAGGACAC AGACTTCTCG
    GTGAAGGAGG ACTGGAAGTA CGTGGCCATG GTCATCGACC GAATCTTCCT CTGGGTGTTC
    ATCATCGTCT GCCTGCTGGG GACCGCGGGC CTCTTCCTGC CCCCCTGGCT GGCCGGCATG
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635345.1
    CDS99..1424
    Translation

    Target ORF information:

    RefSeq Version XM_005635288.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635288.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAGTTCG GGTCCTGGAC GTACGACAAG GCCAAGATAG ACCTGGTGAG CATGCACAGC 
    CGCGTGGACC AGCTGGACCT GTGGGAGAGC GGCGAGTGGG TCATCGTGGA CGCCGTGGGC
    ACCTACAACA CCAGGAAGTA CGAGTGCTGC GCCGAGGTCT ACCCGGACAT CACCTACGCC
    TTCGTCATCC GGCGCCTGCC GCTCTTCTAC ACCATCAACC TCATCATCCC CTGCCTGCTC
    ATCTCCTGCC TCACCGTGCT CGTCTTCTAC CTGCCGTCGG AGTGCGGCGA GAAGATCACG
    CTGTGCATCT CCGTGCTGCT CTCGCTCACC GTCTTCCTGC TGCTCATCAC CGAGATCATC
    CCCTCCACCT CGCTGGTCAT CCCGCTCATC GGCGAGTACC TGCTCTTCAC CATGATCTTC
    GTCACGCTGT CCATCGTCAT CACGGTCTTC GTGCTCAACG TGCACCACCG CTCGCCGCGC
    ACGCACACCA TGCCCGCCTG GGTCCGCCGG GTCTTCCTGG ACGTCGTGCC CCGCCTGCTC
    TTCATGAAGC GGCCGTCCGT GGTCAAGGAC AACTGCAGGC GGCTCATCGA ATCCATGCAC
    AAGATGGCCA GCGCCCCAGG CTTCTGGCCC GAGCCCGAGG GGGAGCCCGG CGTCCCGAGC
    GGAACCCAGA GCCGGGGCCC CTCGCGCACC CCGTCCTTCT GCGGCCCCGT GGACGAGCTG
    GCCAAGCCCC CGCCGGCCTG CAAGTCGCCC TCGGGCCAGG CCCCTGCTCT GCGGCCCTCA
    GAGGCGGAGG AAGCGAGCCC CCGCCCCTCG CCTGACTCCT GCCGTCCCCT CACCAAAGCC
    CGGTCCCTGA GTGTCCAGCA TGTGTCCAGT CCTCCCGAAG GGGCGGAGGA CGGCGTCCGC
    TGCCGGTCCC GGAGCATCCA GTACGGCGGT CCCCAGGACG CCGCCGCCTC TGAAGCTGGG
    GGCCCCGTGG CCGGCTCCCC GGCCTTGCTC AAGGCCAGCT CGGCCGAGCT CCCGGCCCCG
    GAGCAGCCCT CTCCCTGCAA ATGCAGGTGC AAGAAGGAGC CGTCCCCGAG CGCCGTGCCC
    AAAGCCCGGG GCACCAAAGC GCCCCCCCGG CACCTGCCCC TGTCTCCGGC CCTGACGCGG
    GCGGTGGAGG GCGTCCAGTA CATCGCGGAC CACCTGAAGG CGGAGGACAC AGACTTCTCG
    GTGAAGGAGG ACTGGAAGTA CGTGGCCATG GTCATCGACC GAATCTTCCT CTGGGTGTTC
    ATCATCGTCT GCCTGCTGGG GACCGCGGGC CTCTTCCTGC CCCCCTGGCT GGCCGGCATG
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe42930
    Clone ID Related Accession (Same CDS sequence) XM_014107318.1
    Accession Version XM_014107318.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGGCCCA TCTTCCTCCC GTACTTGGAC CCGGCTCCTC CTTCCAGCTT GAGCCAAACC 
    AGCAGCGGAG GGCGGACACG ACACAGTGCA CCCCTTGGAG AGCGGGGAAC CCGTGGGAGG
    TTCCCAGTGA GCAGCCACGT GGAGACCCGG GCCCACGCGG AGGAACGGCT GCTGAAGACG
    CTTTTCTCTG GCTACAACAA GTGGTCCCGG CCCGTGGCCA ACATCTCCGA CGTGGTCCTT
    GTCCACTTCG GGCTGTCCAT CGCGCAGCTC ATTGACGTGG ATGAGAAGAA CCAAATGATG
    ACCACCAACG TGTGGGTGAA GCAGGAGTGG CACGACTATA AGCTGCGCTG GGACCCTGCC
    GATTACGAGA ACGTCACCTC CATCCGCATC CCCTCTGAGC TCATCTGGCG GCCGGACATC
    GTCCTCTACA ACAACGCGGA CGGGGACTTT GCGGTCACCC ACCTGACCAA GGCCCACCTC
    TTCTACGACG GGCGGGTCCA GTGGACACCC CCCGCCATCT ACAAGAGCTC GTGCAGCATC
    GACGTCACCT TCTTCCCGTT CGACCAGCAG AACTGCACCA TGAAGTTCGG GTCCTGGACG
    TACGACAAGG CCAAGATAGA CCTGGTGAGC ATGCACAGCC GCGTGGACCA GCTGGACCTG
    TGGGAGAGCG GCGAGTGGGT CATCGTGGAC GCCGTGGGCA CCTACAACAC CAGGAAGTAC
    GAGTGCTGCG CCGAGGTCTA CCCGGACATC ACCTACGCCT TCGTCATCCG GCGCCTGCCG
    CTCTTCTACA CCATCAACCT CATCATCCCC TGCCTGCTCA TCTCCTGCCT CACCGTGCTC
    GTCTTCTACC TGCCGTCGGA GTGCGGCGAG AAGATCACGC TGTGCATCTC CGTGCTGCTC
    TCGCTCACCG TCTTCCTGCT GCTCATCACC GAGATCATCC CCTCCACCTC GCTGGTCATC
    CCGCTCATCG GCGAGTACCT GCTCTTCACC ATGATCTTCG TCACGCTGTC CATCGTCATC
    ACGGTCTTCG TGCTCAACGT GCACCACCGC TCGCCGCGCA CGCACACCAT GCCCGCCTGG
    GTCCGCCGGG TCTTCCTGGA CGTCGTGCCC CGCCTGCTCT TCATGAAGCG GCCGTCCGTG
    GTCAAGGACA ACTGCAGGCG GCTCATCGAA TCCATGCACA AGATGGCCAG CGCCCCAGGC
    TTCTGGCCCG AGCCCGAGGG GGAGCCCGGC GTCCCGAGCG GAACCCAGAG CCGGGGCCCC
    TCGCGCACCC CGTCCTTCTG CGGCCCCGTG GACGAGCTGG CCAAGCCCCC GCCGGCCTGC
    AAGTCGCCCT CGGGCCAGGC CCCTGCTCTG CGGCCCTCAG AGGCGGAGGA AGCGAGCCCC
    CGCCCCTCGC CTGACTCCTG CCGTCCCCTC ACCAAAGCCC GGTCCCTGAG TGTCCAGCAT
    GTGTCCAGTC CTCCCGAAGG GGCGGAGGAC GGCGTCCGCT GCCGGTCCCG GAGCATCCAG
    TACGGCGGTC CCCAGGACGC CGCCGCCTCT GAAGCTGGGG GCCCCGTGGC CGGCTCCCCG
    GCCTTGCTCA AGGCCAGCTC GGCCGAGCTC CCGGCCCCGG AGCAGCCCTC TCCCTGCAAA
    TGCAGGTGCA AGAAGGAGCC GTCCCCGAGC GCCGTGCCCA AAGCCCGGGG CACCAAAGCG
    CCCCCCCGGC ACCTGCCCCT GTCTCCGGCC CTGACGCGGG CGGTGGAGGG CGTCCAGTAC
    ATCGCGGACC ACCTGAAGGC GGAGGACACA GACTTCTCGG TGAAGGAGGA CTGGAAGTAC
    GTGGCCATGG TCATCGACCG AATCTTCCTC TGGGTGTTCA TCATCGTCTG CCTGCTGGGG
    ACCGCGGGCC TCTTCCTGCC CCCCTGGCTG GCCGGCATGA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013962793.1
    CDS34..1938
    Translation

    Target ORF information:

    RefSeq Version XM_014107318.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014107318.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGGCCCA TCTTCCTCCC GTACTTGGAC CCGGCTCCTC CTTCCAGCTT GAGCCAAACC 
    AGCAGCGGAG GGCGGACACG ACACAGTGCA CCCCTTGGAG AGCGGGGAAC CCGTGGGAGG
    TTCCCAGTGA GCAGCCACGT GGAGACCCGG GCCCACGCGG AGGAACGGCT GCTGAAGACG
    CTTTTCTCTG GCTACAACAA GTGGTCCCGG CCCGTGGCCA ACATCTCCGA CGTGGTCCTT
    GTCCACTTCG GGCTGTCCAT CGCGCAGCTC ATTGACGTGG ATGAGAAGAA CCAAATGATG
    ACCACCAACG TGTGGGTGAA GCAGGAGTGG CACGACTATA AGCTGCGCTG GGACCCTGCC
    GATTACGAGA ACGTCACCTC CATCCGCATC CCCTCTGAGC TCATCTGGCG GCCGGACATC
    GTCCTCTACA ACAACGCGGA CGGGGACTTT GCGGTCACCC ACCTGACCAA GGCCCACCTC
    TTCTACGACG GGCGGGTCCA GTGGACACCC CCCGCCATCT ACAAGAGCTC GTGCAGCATC
    GACGTCACCT TCTTCCCGTT CGACCAGCAG AACTGCACCA TGAAGTTCGG GTCCTGGACG
    TACGACAAGG CCAAGATAGA CCTGGTGAGC ATGCACAGCC GCGTGGACCA GCTGGACCTG
    TGGGAGAGCG GCGAGTGGGT CATCGTGGAC GCCGTGGGCA CCTACAACAC CAGGAAGTAC
    GAGTGCTGCG CCGAGGTCTA CCCGGACATC ACCTACGCCT TCGTCATCCG GCGCCTGCCG
    CTCTTCTACA CCATCAACCT CATCATCCCC TGCCTGCTCA TCTCCTGCCT CACCGTGCTC
    GTCTTCTACC TGCCGTCGGA GTGCGGCGAG AAGATCACGC TGTGCATCTC CGTGCTGCTC
    TCGCTCACCG TCTTCCTGCT GCTCATCACC GAGATCATCC CCTCCACCTC GCTGGTCATC
    CCGCTCATCG GCGAGTACCT GCTCTTCACC ATGATCTTCG TCACGCTGTC CATCGTCATC
    ACGGTCTTCG TGCTCAACGT GCACCACCGC TCGCCGCGCA CGCACACCAT GCCCGCCTGG
    GTCCGCCGGG TCTTCCTGGA CGTCGTGCCC CGCCTGCTCT TCATGAAGCG GCCGTCCGTG
    GTCAAGGACA ACTGCAGGCG GCTCATCGAA TCCATGCACA AGATGGCCAG CGCCCCAGGC
    TTCTGGCCCG AGCCCGAGGG GGAGCCCGGC GTCCCGAGCG GAACCCAGAG CCGGGGCCCC
    TCGCGCACCC CGTCCTTCTG CGGCCCCGTG GACGAGCTGG CCAAGCCCCC GCCGGCCTGC
    AAGTCGCCCT CGGGCCAGGC CCCTGCTCTG CGGCCCTCAG AGGCGGAGGA AGCGAGCCCC
    CGCCCCTCGC CTGACTCCTG CCGTCCCCTC ACCAAAGCCC GGTCCCTGAG TGTCCAGCAT
    GTGTCCAGTC CTCCCGAAGG GGCGGAGGAC GGCGTCCGCT GCCGGTCCCG GAGCATCCAG
    TACGGCGGTC CCCAGGACGC CGCCGCCTCT GAAGCTGGGG GCCCCGTGGC CGGCTCCCCG
    GCCTTGCTCA AGGCCAGCTC GGCCGAGCTC CCGGCCCCGG AGCAGCCCTC TCCCTGCAAA
    TGCAGGTGCA AGAAGGAGCC GTCCCCGAGC GCCGTGCCCA AAGCCCGGGG CACCAAAGCG
    CCCCCCCGGC ACCTGCCCCT GTCTCCGGCC CTGACGCGGG CGGTGGAGGG CGTCCAGTAC
    ATCGCGGACC ACCTGAAGGC GGAGGACACA GACTTCTCGG TGAAGGAGGA CTGGAAGTAC
    GTGGCCATGG TCATCGACCG AATCTTCCTC TGGGTGTTCA TCATCGTCTG CCTGCTGGGG
    ACCGCGGGCC TCTTCCTGCC CCCCTGGCTG GCCGGCATGA TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25179
    Clone ID Related Accession (Same CDS sequence) XM_543097.5
    Accession Version XM_543097.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neuronal acetylcholine receptor subunit alpha-4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_543097.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCTCG GGGGCCCCGG GGCGCCGCCG CCGCCGCTGC CGGCGCTGCT GCTGCTGCTG 
    GGGGCCGGCT TCCTGCCCGT GAGCAGCCAC GTGGAGACCC GGGCCCACGC GGAGGAACGG
    CTGCTGAAGA CGCTTTTCTC TGGCTACAAC AAGTGGTCCC GGCCCGTGGC CAACATCTCC
    GACGTGGTCC TTGTCCACTT CGGGCTGTCC ATCGCGCAGC TCATTGACGT GGATGAGAAG
    AACCAAATGA TGACCACCAA CGTGTGGGTG AAGCAGGAGT GGCACGACTA TAAGCTGCGC
    TGGGACCCTG CCGATTACGA GAACGTCACC TCCATCCGCA TCCCCTCTGA GCTCATCTGG
    CGGCCGGACA TCGTCCTCTA CAACAACGCG GACGGGGACT TTGCGGTCAC CCACCTGACC
    AAGGCCCACC TCTTCTACGA CGGGCGGGTC CAGTGGACAC CCCCCGCCAT CTACAAGAGC
    TCGTGCAGCA TCGACGTCAC CTTCTTCCCG TTCGACCAGC AGAACTGCAC CATGAAGTTC
    GGGTCCTGGA CGTACGACAA GGCCAAGATA GACCTGGTGA GCATGCACAG CCGCGTGGAC
    CAGCTGGACC TGTGGGAGAG CGGCGAGTGG GTCATCGTGG ACGCCGTGGG CACCTACAAC
    ACCAGGAAGT ACGAGTGCTG CGCCGAGGTC TACCCGGACA TCACCTACGC CTTCGTCATC
    CGGCGCCTGC CGCTCTTCTA CACCATCAAC CTCATCATCC CCTGCCTGCT CATCTCCTGC
    CTCACCGTGC TCGTCTTCTA CCTGCCGTCG GAGTGCGGCG AGAAGATCAC GCTGTGCATC
    TCCGTGCTGC TCTCGCTCAC CGTCTTCCTG CTGCTCATCA CCGAGATCAT CCCCTCCACC
    TCGCTGGTCA TCCCGCTCAT CGGCGAGTAC CTGCTCTTCA CCATGATCTT CGTCACGCTG
    TCCATCGTCA TCACGGTCTT CGTGCTCAAC GTGCACCACC GCTCGCCGCG CACGCACACC
    ATGCCCGCCT GGGTCCGCCG GGTCTTCCTG GACGTCGTGC CCCGCCTGCT CTTCATGAAG
    CGGCCGTCCG TGGTCAAGGA CAACTGCAGG CGGCTCATCG AATCCATGCA CAAGATGGCC
    AGCGCCCCAG GCTTCTGGCC CGAGCCCGAG GGGGAGCCCG GCGTCCCGAG CGGAACCCAG
    AGCCGGGGCC CCTCGCGCAC CCCGTCCTTC TGCGGCCCCG TGGACGAGCT GGCCAAGCCC
    CCGCCGGCCT GCAAGTCGCC CTCGGGCCAG GCCCCTGCTC TGCGGCCCTC AGAGGCGGAG
    GAAGCGAGCC CCCGCCCCTC GCCTGACTCC TGCCGTCCCC TCACCAAAGC CCGGTCCCTG
    AGTGTCCAGC ATGTGTCCAG TCCTCCCGAA GGGGCGGAGG ACGGCGTCCG CTGCCGGTCC
    CGGAGCATCC AGTACGGCGG TCCCCAGGAC GCCGCCGCCT CTGAAGCTGG GGGCCCCGTG
    GCCGGCTCCC CGGCCTTGCT CAAGGCCAGC TCGGCCGAGC TCCCGGCCCC GGAGCAGCCC
    TCTCCCTGCA AATGCAGGTG CAAGAAGGAG CCGTCCCCGA GCGCCGTGCC CAAAGCCCGG
    GGCACCAAAG CGCCCCCCCG GCACCTGCCC CTGTCTCCGG CCCTGACGCG GGCGGTGGAG
    GGCGTCCAGT ACATCGCGGA CCACCTGAAG GCGGAGGACA CAGACTTCTC GGTGAAGGAG
    GACTGGAAGT ACGTGGCCAT GGTCATCGAC CGAATCTTCC TCTGGGTGTT CATCATCGTC
    TGCCTGCTGG GGACCGCGGG CCTCTTCCTG CCCCCCTGGC TGGCCGGCAT GATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_543097.3
    CDS204..2060
    Translation

    Target ORF information:

    RefSeq Version XM_543097.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris cholinergic receptor, nicotinic, alpha 4 (neuronal) (CHRNA4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_543097.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAGCTCG GGGGCCCCGG GGCGCCGCCG CCGCCGCTGC CGGCGCTGCT GCTGCTGCTG 
    GGGGCCGGCT TCCTGCCCGT GAGCAGCCAC GTGGAGACCC GGGCCCACGC GGAGGAACGG
    CTGCTGAAGA CGCTTTTCTC TGGCTACAAC AAGTGGTCCC GGCCCGTGGC CAACATCTCC
    GACGTGGTCC TTGTCCACTT CGGGCTGTCC ATCGCGCAGC TCATTGACGT GGATGAGAAG
    AACCAAATGA TGACCACCAA CGTGTGGGTG AAGCAGGAGT GGCACGACTA TAAGCTGCGC
    TGGGACCCTG CCGATTACGA GAACGTCACC TCCATCCGCA TCCCCTCTGA GCTCATCTGG
    CGGCCGGACA TCGTCCTCTA CAACAACGCG GACGGGGACT TTGCGGTCAC CCACCTGACC
    AAGGCCCACC TCTTCTACGA CGGGCGGGTC CAGTGGACAC CCCCCGCCAT CTACAAGAGC
    TCGTGCAGCA TCGACGTCAC CTTCTTCCCG TTCGACCAGC AGAACTGCAC CATGAAGTTC
    GGGTCCTGGA CGTACGACAA GGCCAAGATA GACCTGGTGA GCATGCACAG CCGCGTGGAC
    CAGCTGGACC TGTGGGAGAG CGGCGAGTGG GTCATCGTGG ACGCCGTGGG CACCTACAAC
    ACCAGGAAGT ACGAGTGCTG CGCCGAGGTC TACCCGGACA TCACCTACGC CTTCGTCATC
    CGGCGCCTGC CGCTCTTCTA CACCATCAAC CTCATCATCC CCTGCCTGCT CATCTCCTGC
    CTCACCGTGC TCGTCTTCTA CCTGCCGTCG GAGTGCGGCG AGAAGATCAC GCTGTGCATC
    TCCGTGCTGC TCTCGCTCAC CGTCTTCCTG CTGCTCATCA CCGAGATCAT CCCCTCCACC
    TCGCTGGTCA TCCCGCTCAT CGGCGAGTAC CTGCTCTTCA CCATGATCTT CGTCACGCTG
    TCCATCGTCA TCACGGTCTT CGTGCTCAAC GTGCACCACC GCTCGCCGCG CACGCACACC
    ATGCCCGCCT GGGTCCGCCG GGTCTTCCTG GACGTCGTGC CCCGCCTGCT CTTCATGAAG
    CGGCCGTCCG TGGTCAAGGA CAACTGCAGG CGGCTCATCG AATCCATGCA CAAGATGGCC
    AGCGCCCCAG GCTTCTGGCC CGAGCCCGAG GGGGAGCCCG GCGTCCCGAG CGGAACCCAG
    AGCCGGGGCC CCTCGCGCAC CCCGTCCTTC TGCGGCCCCG TGGACGAGCT GGCCAAGCCC
    CCGCCGGCCT GCAAGTCGCC CTCGGGCCAG GCCCCTGCTC TGCGGCCCTC AGAGGCGGAG
    GAAGCGAGCC CCCGCCCCTC GCCTGACTCC TGCCGTCCCC TCACCAAAGC CCGGTCCCTG
    AGTGTCCAGC ATGTGTCCAG TCCTCCCGAA GGGGCGGAGG ACGGCGTCCG CTGCCGGTCC
    CGGAGCATCC AGTACGGCGG TCCCCAGGAC GCCGCCGCCT CTGAAGCTGG GGGCCCCGTG
    GCCGGCTCCC CGGCCTTGCT CAAGGCCAGC TCGGCCGAGC TCCCGGCCCC GGAGCAGCCC
    TCTCCCTGCA AATGCAGGTG CAAGAAGGAG CCGTCCCCGA GCGCCGTGCC CAAAGCCCGG
    GGCACCAAAG CGCCCCCCCG GCACCTGCCC CTGTCTCCGG CCCTGACGCG GGCGGTGGAG
    GGCGTCCAGT ACATCGCGGA CCACCTGAAG GCGGAGGACA CAGACTTCTC GGTGAAGGAG
    GACTGGAAGT ACGTGGCCAT GGTCATCGAC CGAATCTTCC TCTGGGTGTT CATCATCGTC
    TGCCTGCTGG GGACCGCGGG CCTCTTCCTG CCCCCCTGGC TGGCCGGCAT GATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe296851
    Clone ID Related Accession (Same CDS sequence) XM_022409244.1
    Accession Version XM_022409244.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LOW QUALITY PROTEIN: neuronal acetylcholine receptor subunit alpha-4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCTCG GGGGCCCCGG GGCGCCGCCG CCGCCGCTGC CGGCGCTGCT GCTGCTGCTG 
    GGGGCCGGCT TCCTGCCCGT GAGCAGCCAC GTGGAGACCC GGGCCCACGC GGAGGAACGG
    CTGCTGAAGA CGCTTTTCTC TGGCTACAAC AAGTGGTCCC GGCCCGTGGC CAACATCTCC
    GACGTGGTCC TTGTCCACTT CGGGCTGTCC ATCGCGCAGC TCATTGACGT GGATGAGAAG
    AACCAAATGA TGACCACCAA CGTGTGGGTG AAGCAGGTGA GTGAGGGGGA TGGGGGGCCT
    CCACCCCTGG TTCCTCCCCG CCCTCCCCCC ACCCTCCCCT CACCGCGGCC TCCCCCTGCC
    CTGGCCCAGG AGTGGCACGA CTATAAGCTG CGCTGGGACC CTGCCGATTA CGAGAACGTC
    ACCTCCATCC GCATCCCCTC TGAGCTCATC TGGCGGCCGG ACATCGTCCT CTACAACAAC
    GCGGACGGGG ACTTTGCGGT CACCCACCTG ACCAAGGCCC ACCTCTTCTA CGACGGGCGG
    GTCCAGTGGA CACCCCCCGC CATCTACAAG AGCTCGTGCA GCATCGACGT CACCTTCTTC
    CCGTTCGACC AGCAGAACTG CACCATGAAG TTCGGGTCCT GGACGTACGA CAAGGCCAAG
    ATAGACCTGG TGAGCATGCA CAGCCGCGTG GACCAGCTGG ACCTGTGGGA GAGCGGCGAG
    TGGGTCATCG TGGACGCCGT GGGCACCTAC AACACCAGGA AGTACGAGTG CTGCGCCGAG
    GTCTACCCGG ACATCACCTA CGCCTTCGTC ATCCGGCGCC TGCCGCTCTT CTACACCATC
    AACCTCATCA TCCCCTGCCT GCTCATCTCC TGCCTCACCG TGCTCGTCTT CTACCTGCCG
    TCGGAGTGCG GCGAGAAGAT CACGCTGTGC ATCTCCGTGC TGCTCTCGCT CACCGTCTTC
    CTGCTGCTCA TCACCGAGAT CATCCCCTCC ACCTCGCTGG TCATCCCGCT CATCGGCGAG
    TACCTGCTCT TCACCATGAT CTTCGTCACG CTGTCCATCG TCATCACGGT CTTCGTGCTC
    AACGTGCACC ACCGCTCGCC GCGCACGCAC ACCATGCCCG CCTGGGTCCG CCGGGTCTTC
    CTGGACGTCG TGCCCCGCCT GCTCTTCATG AAGCGGCCGT CCGTGGTCAA GGACAACTGC
    AGGCGGCTCA TCGAATCCAT GCACAAGATG GCCAGCGCCC CAGGCTTCTG GCCCGAGCCC
    GAGGGGGAGC CCGGCGTCCC GAGCGGAACC CAGAGCCGGG GCCCCTCGCG CACCCCGTCC
    TTCTGCGGCC CCGTGGACGA GCTGGCCAAG CCCCCGCCGG CCTGCAAGTC GCCCTCGGGC
    CAGGCCCCTG CTCTGCGGCC CTCAGAGGCG GAGGAAGCGA GCCCCCGCCC CTCGCCTGAC
    TCCTGCCGTC CCCTCACCAA AGCCCGGTCC CTGAGTGTCC AGCATGTGTC CAGTCCTCCC
    GAAGGGGCGG AGGACGGCGT CCGCTGCCGG TCCCGGAGCA TCCAGTACGG CGGTCCCCAG
    GACGCCGCCG CCTCTGAAGC TGGGGGCCCC GTGGCCGGCT CCCCGGCCTT GCTCAAGGCC
    AGCTCGGCCG AGCTCCCGGC CCCGGAGCAG CCCTCTCCCT GCAAATGCAG GTGCAAGAAG
    GAGCCGTCCC CGAGCGCCGT GCCCAAAGCC CGGGGCACCA AAGCGCCCCC CCGGCACCTG
    CCCCTGTCTC CGGCCCTGAC GCGGGCGGTG GAGGGCGTCC AGTACATCGC GGACCACCTG
    AAGGCGGAGG ACACAGACTT CTCGGTGAAG GAGGACTGGA AGTACGTGGC CATGGTCATC
    GACCGAATCT TCCTCTGGGT GTTCATCATC GTCTGCCTGC TGGGGACCGC GGGCCTCTTC
    CTGCCCCCCT GGCTGGCCGG CATGATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022264952.1
    CDS77..2026
    Translation

    Target ORF information:

    RefSeq Version XM_022409244.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris cholinergic receptor nicotinic alpha 4 subunit (CHRNA4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCTCG GGGGCCCCGG GGCGCCGCCG CCGCCGCTGC CGGCGCTGCT GCTGCTGCTG 
    GGGGCCGGCT TCCTGCCCGT GAGCAGCCAC GTGGAGACCC GGGCCCACGC GGAGGAACGG
    CTGCTGAAGA CGCTTTTCTC TGGCTACAAC AAGTGGTCCC GGCCCGTGGC CAACATCTCC
    GACGTGGTCC TTGTCCACTT CGGGCTGTCC ATCGCGCAGC TCATTGACGT GGATGAGAAG
    AACCAAATGA TGACCACCAA CGTGTGGGTG AAGCAGGTGA GTGAGGGGGA TGGGGGGCCT
    CCACCCCTGG TTCCTCCCCG CCCTCCCCCC ACCCTCCCCT CACCGCGGCC TCCCCCTGCC
    CTGGCCCAGG AGTGGCACGA CTATAAGCTG CGCTGGGACC CTGCCGATTA CGAGAACGTC
    ACCTCCATCC GCATCCCCTC TGAGCTCATC TGGCGGCCGG ACATCGTCCT CTACAACAAC
    GCGGACGGGG ACTTTGCGGT CACCCACCTG ACCAAGGCCC ACCTCTTCTA CGACGGGCGG
    GTCCAGTGGA CACCCCCCGC CATCTACAAG AGCTCGTGCA GCATCGACGT CACCTTCTTC
    CCGTTCGACC AGCAGAACTG CACCATGAAG TTCGGGTCCT GGACGTACGA CAAGGCCAAG
    ATAGACCTGG TGAGCATGCA CAGCCGCGTG GACCAGCTGG ACCTGTGGGA GAGCGGCGAG
    TGGGTCATCG TGGACGCCGT GGGCACCTAC AACACCAGGA AGTACGAGTG CTGCGCCGAG
    GTCTACCCGG ACATCACCTA CGCCTTCGTC ATCCGGCGCC TGCCGCTCTT CTACACCATC
    AACCTCATCA TCCCCTGCCT GCTCATCTCC TGCCTCACCG TGCTCGTCTT CTACCTGCCG
    TCGGAGTGCG GCGAGAAGAT CACGCTGTGC ATCTCCGTGC TGCTCTCGCT CACCGTCTTC
    CTGCTGCTCA TCACCGAGAT CATCCCCTCC ACCTCGCTGG TCATCCCGCT CATCGGCGAG
    TACCTGCTCT TCACCATGAT CTTCGTCACG CTGTCCATCG TCATCACGGT CTTCGTGCTC
    AACGTGCACC ACCGCTCGCC GCGCACGCAC ACCATGCCCG CCTGGGTCCG CCGGGTCTTC
    CTGGACGTCG TGCCCCGCCT GCTCTTCATG AAGCGGCCGT CCGTGGTCAA GGACAACTGC
    AGGCGGCTCA TCGAATCCAT GCACAAGATG GCCAGCGCCC CAGGCTTCTG GCCCGAGCCC
    GAGGGGGAGC CCGGCGTCCC GAGCGGAACC CAGAGCCGGG GCCCCTCGCG CACCCCGTCC
    TTCTGCGGCC CCGTGGACGA GCTGGCCAAG CCCCCGCCGG CCTGCAAGTC GCCCTCGGGC
    CAGGCCCCTG CTCTGCGGCC CTCAGAGGCG GAGGAAGCGA GCCCCCGCCC CTCGCCTGAC
    TCCTGCCGTC CCCTCACCAA AGCCCGGTCC CTGAGTGTCC AGCATGTGTC CAGTCCTCCC
    GAAGGGGCGG AGGACGGCGT CCGCTGCCGG TCCCGGAGCA TCCAGTACGG CGGTCCCCAG
    GACGCCGCCG CCTCTGAAGC TGGGGGCCCC GTGGCCGGCT CCCCGGCCTT GCTCAAGGCC
    AGCTCGGCCG AGCTCCCGGC CCCGGAGCAG CCCTCTCCCT GCAAATGCAG GTGCAAGAAG
    GAGCCGTCCC CGAGCGCCGT GCCCAAAGCC CGGGGCACCA AAGCGCCCCC CCGGCACCTG
    CCCCTGTCTC CGGCCCTGAC GCGGGCGGTG GAGGGCGTCC AGTACATCGC GGACCACCTG
    AAGGCGGAGG ACACAGACTT CTCGGTGAAG GAGGACTGGA AGTACGTGGC CATGGTCATC
    GACCGAATCT TCCTCTGGGT GTTCATCATC GTCTGCCTGC TGGGGACCGC GGGCCTCTTC
    CTGCCCCCCT GGCTGGCCGG CATGATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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