PHLDB1 cDNA ORF clone, Canis lupus familiaris(dog)

  • Gene

  • Clones

  • gRNAs

The following PHLDB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHLDB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287945 XM_022418237.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287946 XM_022418238.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe05896 XM_005619707.2
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe05899 XM_855372.5
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe05888 XM_005619699.2
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe05889 XM_005619700.2
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe05890 XM_005619701.2
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe05891 XM_005619702.2
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe05892 XM_005619703.2
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe05893 XM_005619704.2
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe05894 XM_005619705.2
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe05895 XM_005619706.2
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe05897 XM_546499.5
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe05898 XM_005619708.3
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe287933 XM_022418225.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287934 XM_022418226.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287935 XM_022418227.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287936 XM_022418228.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287937 XM_022418229.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287938 XM_022418230.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287939 XM_022418231.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287940 XM_022418232.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287941 XM_022418233.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287942 XM_022418234.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe287943 XM_022418235.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe287944 XM_022418236.1
Latest version!
Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe36310 XM_014113376.1
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe36311 XM_014113377.1
Latest version!
Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OCe05897 XM_546499.4 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OCe05899 XM_855372.4 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe05898 XM_005619708.2 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287945
    Clone ID Related Accession (Same CDS sequence) XM_022418237.1
    Accession Version XM_022418237.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4362bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GTTTACCGCT CCAAGATGGA TGGTGAGGCC ACCAGCCCCC TGCCCCGGAC CCGCAGTGGC
    CCCCTCCCCT CCTCCTCTGG TTCTTCTTCC TCTTCTTCGC AGCTCAGCGT GGCTACTCTG
    GGCCGGAGCC CCTCCCCAAA GAGTGCCCTA CTTGCCCAGA ATGGCACAGG CAGCCTTCCT
    CGCAACCTGG CGGCCACGCT GCAGGACATT GAGACCAAGC GCCAGCTGGC CCTGCAGCAG
    AAGGGCGAGT CGCTTCCTGC AGAGCCCCCC CCAGCAGACG ACCCAGCAGG ACAGCAGGTG
    ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG
    TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC
    CCGCTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG
    GGCGAGCACG CCTACGACAC CCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC
    TCCACAGGCG GCAACTCAGC CTGCTCGCCC GACAACATGT CCAGTGCGAG CGGCCTGGAT
    GTGGGCAAAA TCGAGGAGAT GGAGAAGATG CTGAAAGAGG CACACGCCGA GAAGAGCCGG
    CTCATGGAGT CCAGGGAGCG GGAGATGGAG CTCCGGCGGC AGGCCCTGGA GGAGGAACGG
    AGGAGGCGAG AACAGGTGGA ACGGAGGCTA CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG
    GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA CAGTTTTCCC AGGCACGACC CCTGACCCGC
    TACCTGCCAA TCCGGAAGGA GGACTTTGAC CTGAAGACCC ACATCGAGTC GTCGGGCCAC
    GGTGTCGATA CCTGCCTGCA CGTGGTGCTT AGCAGCAAGG TCTGCCGCGG CTACTTGGTC
    AAGATGGGCG GCAAGATTAA ATCATGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG
    CGCACCCTTT CCTATTATGT GGACAAGCAC GAGACGAAGC TGAAGGGGGT CATCTACTTC
    CAGGCCATCG AGGAAGTGTA CTACGATCAC CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC
    AGCTTTTCTG TGGTGACTGA GAGCCCGAAC CCAGCCCTCA CCTTCTGTGT GAAGACCCAC
    GACCGGCTGT ATTACATGGT GGCCCCGTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC
    ATCGTCACGG GAGCGGAGGG CTACACTCAG TTCATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273945.1
    CDS89..4450
    Translation

    Target ORF information:

    RefSeq Version XM_022418237.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GTTTACCGCT CCAAGATGGA TGGTGAGGCC ACCAGCCCCC TGCCCCGGAC CCGCAGTGGC
    CCCCTCCCCT CCTCCTCTGG TTCTTCTTCC TCTTCTTCGC AGCTCAGCGT GGCTACTCTG
    GGCCGGAGCC CCTCCCCAAA GAGTGCCCTA CTTGCCCAGA ATGGCACAGG CAGCCTTCCT
    CGCAACCTGG CGGCCACGCT GCAGGACATT GAGACCAAGC GCCAGCTGGC CCTGCAGCAG
    AAGGGCGAGT CGCTTCCTGC AGAGCCCCCC CCAGCAGACG ACCCAGCAGG ACAGCAGGTG
    ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG
    TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC
    CCGCTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG
    GGCGAGCACG CCTACGACAC CCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC
    TCCACAGGCG GCAACTCAGC CTGCTCGCCC GACAACATGT CCAGTGCGAG CGGCCTGGAT
    GTGGGCAAAA TCGAGGAGAT GGAGAAGATG CTGAAAGAGG CACACGCCGA GAAGAGCCGG
    CTCATGGAGT CCAGGGAGCG GGAGATGGAG CTCCGGCGGC AGGCCCTGGA GGAGGAACGG
    AGGAGGCGAG AACAGGTGGA ACGGAGGCTA CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG
    GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA CAGTTTTCCC AGGCACGACC CCTGACCCGC
    TACCTGCCAA TCCGGAAGGA GGACTTTGAC CTGAAGACCC ACATCGAGTC GTCGGGCCAC
    GGTGTCGATA CCTGCCTGCA CGTGGTGCTT AGCAGCAAGG TCTGCCGCGG CTACTTGGTC
    AAGATGGGCG GCAAGATTAA ATCATGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG
    CGCACCCTTT CCTATTATGT GGACAAGCAC GAGACGAAGC TGAAGGGGGT CATCTACTTC
    CAGGCCATCG AGGAAGTGTA CTACGATCAC CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC
    AGCTTTTCTG TGGTGACTGA GAGCCCGAAC CCAGCCCTCA CCTTCTGTGT GAAGACCCAC
    GACCGGCTGT ATTACATGGT GGCCCCGTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC
    ATCGTCACGG GAGCGGAGGG CTACACTCAG TTCATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287946
    Clone ID Related Accession (Same CDS sequence) XM_022418238.1
    Accession Version XM_022418238.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC
    CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG
    AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC
    CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC
    GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA GACGACCCAG CAGGACAGCA GGTGATCGAG
    GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG
    TGGGACGCCC TGCACGGGGC GGCCCCCTTC CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC
    ATGCACCACT CCATCCTGCA CCACCTGCCG GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG
    CACGCCTACG ACACCCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACA
    GGCGGCAACT CAGCCTGCTC GCCCGACAAC ATGTCCAGTG CGAGCGGCCT GGATGTGGGC
    AAAATCGAGG AGATGGAGAA GATGCTGAAA GAGGCACACG CCGAGAAGAG CCGGCTCATG
    GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG
    CGAGAACAGG TGGAACGGAG GCTACAGAGC GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG
    AAGGAGGTCA AGATGCGAGA GAAACAGTTT TCCCAGGCAC GACCCCTGAC CCGCTACCTG
    CCAATCCGGA AGGAGGACTT TGACCTGAAG ACCCACATCG AGTCGTCGGG CCACGGTGTC
    GATACCTGCC TGCACGTGGT GCTTAGCAGC AAGGTCTGCC GCGGCTACTT GGTCAAGATG
    GGCGGCAAGA TTAAATCATG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC
    CTTTCCTATT ATGTGGACAA GCACGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC
    ATCGAGGAAG TGTACTACGA TCACCTGCGC AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT
    TCTGTGGTGA CTGAGAGCCC GAACCCAGCC CTCACCTTCT GTGTGAAGAC CCACGACCGG
    CTGTATTACA TGGTGGCCCC GTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATCGTC
    ACGGGAGCGG AGGGCTACAC TCAGTTCATG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273946.1
    CDS86..4201
    Translation

    Target ORF information:

    RefSeq Version XM_022418238.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC
    CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG
    AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC
    CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC
    GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA GACGACCCAG CAGGACAGCA GGTGATCGAG
    GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG
    TGGGACGCCC TGCACGGGGC GGCCCCCTTC CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC
    ATGCACCACT CCATCCTGCA CCACCTGCCG GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG
    CACGCCTACG ACACCCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACA
    GGCGGCAACT CAGCCTGCTC GCCCGACAAC ATGTCCAGTG CGAGCGGCCT GGATGTGGGC
    AAAATCGAGG AGATGGAGAA GATGCTGAAA GAGGCACACG CCGAGAAGAG CCGGCTCATG
    GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG
    CGAGAACAGG TGGAACGGAG GCTACAGAGC GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG
    AAGGAGGTCA AGATGCGAGA GAAACAGTTT TCCCAGGCAC GACCCCTGAC CCGCTACCTG
    CCAATCCGGA AGGAGGACTT TGACCTGAAG ACCCACATCG AGTCGTCGGG CCACGGTGTC
    GATACCTGCC TGCACGTGGT GCTTAGCAGC AAGGTCTGCC GCGGCTACTT GGTCAAGATG
    GGCGGCAAGA TTAAATCATG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC
    CTTTCCTATT ATGTGGACAA GCACGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC
    ATCGAGGAAG TGTACTACGA TCACCTGCGC AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT
    TCTGTGGTGA CTGAGAGCCC GAACCCAGCC CTCACCTTCT GTGTGAAGAC CCACGACCGG
    CTGTATTACA TGGTGGCCCC GTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATCGTC
    ACGGGAGCGG AGGGCTACAC TCAGTTCATG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05896
    Clone ID Related Accession (Same CDS sequence) XM_005619707.2
    Accession Version XM_005619707.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619707.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGTTTACC GCTCCAAGAT GGATGGTGAG GCCACCAGCC CCCTGCCCCG GACCCGCAGT
    GGCCCCCTCC CCTCCTCCTC TGGTTCTTCT TCCTCTTCTT CGCAGCTCAG CGTGGCTACT
    CTGGGCCGGA GCCCCTCCCC AAAGAGTGCC CTACTTGCCC AGAATGGCAC AGGCAGCCTT
    CCTCGCAACC TGGCGGCCAC GCTGCAGGAC ATTGAGACCA AGCGCCAGCT GGCCCTGCAG
    CAGAAGGGCG AGTCGCTTCC TGCAGAGCCC CCCCCAGCAG ACGACCCAGC AGGACAGCAG
    GTGATCGAGG AGCAGCGGCG GCGCCTGGCC GAGCTGAAGC AGAAGGCTGC GGCCGAGGCG
    CAGTGCCAGT GGGACGCCCT GCACGGGGCG GCCCCCTTCC CCCCGGGCCC CTCGGGCTTC
    CCCCCGCTCA TGCACCACTC CATCCTGCAC CACCTGCCGG CTGGCCGGGA GCGCGGGGAG
    GAGGGCGAGC ACGCCTACGA CACCCTGAGC CTGGAGAGCT CCGACAGCAT GGAGACCAGC
    ATCTCCACAG GCGGCAACTC AGCCTGCTCG CCCGACAACA TGTCCAGTGC GAGCGGCCTG
    GATGTGGGCA AAATCGAGGA GATGGAGAAG ATGCTGAAAG AGGCACACGC CGAGAAGAGC
    CGGCTCATGG AGTCCAGGGA GCGGGAGATG GAGCTCCGGC GGCAGGCCCT GGAGGAGGAA
    CGGAGGAGGC GAGAACAGGT GGAACGGAGG CTACAGAGCG AGAGCGCCAG GAGGCAGCAG
    CTGGTGGAGA AGGAGGTCAA GATGCGAGAG AAACAGTTTT CCCAGGCACG ACCCCTGACC
    CGCTACCTGC CAATCCGGAA GGAGGACTTT GACCTGAAGA CCCACATCGA GTCGTCGGGC
    CACGGTGTCG ATACCTGCCT GCACGTGGTG CTTAGCAGCA AGGTCTGCCG CGGCTACTTG
    GTCAAGATGG GCGGCAAGAT TAAATCATGG AAGAAGCGCT GGTTTGTCTT CGACCGGCTC
    AAGCGCACCC TTTCCTATTA TGTGGACAAG CACGAGACGA AGCTGAAGGG GGTCATCTAC
    TTCCAGGCCA TCGAGGAAGT GTACTACGAT CACCTGCGCA GTGCAGCCAA GAGGAGGTTT
    TTCAGCTTTT CTGTGGTGAC TGAGAGCCCG AACCCAGCCC TCACCTTCTG TGTGAAGACC
    CACGACCGGC TGTATTACAT GGTGGCCCCG TCTGCAGAGG CCATGCGCAT CTGGATGGAC
    GTCATCGTCA CGGGAGCGGA GGGCTACACT CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619764.1
    CDS412..4656
    Translation

    Target ORF information:

    RefSeq Version XM_005619707.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619707.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGTTTACC GCTCCAAGAT GGATGGTGAG GCCACCAGCC CCCTGCCCCG GACCCGCAGT
    GGCCCCCTCC CCTCCTCCTC TGGTTCTTCT TCCTCTTCTT CGCAGCTCAG CGTGGCTACT
    CTGGGCCGGA GCCCCTCCCC AAAGAGTGCC CTACTTGCCC AGAATGGCAC AGGCAGCCTT
    CCTCGCAACC TGGCGGCCAC GCTGCAGGAC ATTGAGACCA AGCGCCAGCT GGCCCTGCAG
    CAGAAGGGCG AGTCGCTTCC TGCAGAGCCC CCCCCAGCAG ACGACCCAGC AGGACAGCAG
    GTGATCGAGG AGCAGCGGCG GCGCCTGGCC GAGCTGAAGC AGAAGGCTGC GGCCGAGGCG
    CAGTGCCAGT GGGACGCCCT GCACGGGGCG GCCCCCTTCC CCCCGGGCCC CTCGGGCTTC
    CCCCCGCTCA TGCACCACTC CATCCTGCAC CACCTGCCGG CTGGCCGGGA GCGCGGGGAG
    GAGGGCGAGC ACGCCTACGA CACCCTGAGC CTGGAGAGCT CCGACAGCAT GGAGACCAGC
    ATCTCCACAG GCGGCAACTC AGCCTGCTCG CCCGACAACA TGTCCAGTGC GAGCGGCCTG
    GATGTGGGCA AAATCGAGGA GATGGAGAAG ATGCTGAAAG AGGCACACGC CGAGAAGAGC
    CGGCTCATGG AGTCCAGGGA GCGGGAGATG GAGCTCCGGC GGCAGGCCCT GGAGGAGGAA
    CGGAGGAGGC GAGAACAGGT GGAACGGAGG CTACAGAGCG AGAGCGCCAG GAGGCAGCAG
    CTGGTGGAGA AGGAGGTCAA GATGCGAGAG AAACAGTTTT CCCAGGCACG ACCCCTGACC
    CGCTACCTGC CAATCCGGAA GGAGGACTTT GACCTGAAGA CCCACATCGA GTCGTCGGGC
    CACGGTGTCG ATACCTGCCT GCACGTGGTG CTTAGCAGCA AGGTCTGCCG CGGCTACTTG
    GTCAAGATGG GCGGCAAGAT TAAATCATGG AAGAAGCGCT GGTTTGTCTT CGACCGGCTC
    AAGCGCACCC TTTCCTATTA TGTGGACAAG CACGAGACGA AGCTGAAGGG GGTCATCTAC
    TTCCAGGCCA TCGAGGAAGT GTACTACGAT CACCTGCGCA GTGCAGCCAA GAGGAGGTTT
    TTCAGCTTTT CTGTGGTGAC TGAGAGCCCG AACCCAGCCC TCACCTTCTG TGTGAAGACC
    CACGACCGGC TGTATTACAT GGTGGCCCCG TCTGCAGAGG CCATGCGCAT CTGGATGGAC
    GTCATCGTCA CGGGAGCGGA GGGCTACACT CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05899
    Clone ID Related Accession (Same CDS sequence) XM_855372.4 , XM_855372.5
    Accession Version XM_855372.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_855372.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC
    CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG
    AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC
    CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC
    GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG
    GGCTTCCCCC CGCTCATGCA CCACTCCATC CTGCACCACC TGCCGGCTGG CCGGGAGCGC
    GGGGAGGAGG GCGAGCACGC CTACGACACC CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACAGGCGG CAACTCAGCC TGCTCGCCCG ACAACATGTC CAGTGCGAGC
    GGCCTGGATG TGGGCAAAAT CGAGGAGATG GAGAAGATGC TGAAAGAGGC ACACGCCGAG
    AAGAGCCGGC TCATGGAGTC CAGGGAGCGG GAGATGGAGC TCCGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGAGGCGAGA ACAGGTGGAA CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG
    CAGCAGCTGG TGGAGAAGGA GGTCAAGATG CGAGAGAAAC AGTTTTCCCA GGCACGACCC
    CTGACCCGCT ACCTGCCAAT CCGGAAGGAG GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCGGGCCACG GTGTCGATAC CTGCCTGCAC GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC
    TACTTGGTCA AGATGGGCGG CAAGATTAAA TCATGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTATGTG GACAAGCACG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAAGTGTAC TACGATCACC TGCGCAGTGC AGCCAAGAGC
    CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC
    CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC
    ACTCAGTTCA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_860465.3
    CDS406..4443
    Translation

    Target ORF information:

    RefSeq Version XM_855372.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_855372.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC
    CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG
    AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC
    CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC
    GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG
    GGCTTCCCCC CGCTCATGCA CCACTCCATC CTGCACCACC TGCCGGCTGG CCGGGAGCGC
    GGGGAGGAGG GCGAGCACGC CTACGACACC CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACAGGCGG CAACTCAGCC TGCTCGCCCG ACAACATGTC CAGTGCGAGC
    GGCCTGGATG TGGGCAAAAT CGAGGAGATG GAGAAGATGC TGAAAGAGGC ACACGCCGAG
    AAGAGCCGGC TCATGGAGTC CAGGGAGCGG GAGATGGAGC TCCGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGAGGCGAGA ACAGGTGGAA CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG
    CAGCAGCTGG TGGAGAAGGA GGTCAAGATG CGAGAGAAAC AGTTTTCCCA GGCACGACCC
    CTGACCCGCT ACCTGCCAAT CCGGAAGGAG GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCGGGCCACG GTGTCGATAC CTGCCTGCAC GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC
    TACTTGGTCA AGATGGGCGG CAAGATTAAA TCATGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTATGTG GACAAGCACG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAAGTGTAC TACGATCACC TGCGCAGTGC AGCCAAGAGC
    CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC
    CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC
    ACTCAGTTCA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05899
    Clone ID Related Accession (Same CDS sequence) XM_855372.4 , XM_855372.5
    Accession Version XM_855372.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_855372.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC
    CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG
    AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC
    CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC
    GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG
    GGCTTCCCCC CGCTCATGCA CCACTCCATC CTGCACCACC TGCCGGCTGG CCGGGAGCGC
    GGGGAGGAGG GCGAGCACGC CTACGACACC CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACAGGCGG CAACTCAGCC TGCTCGCCCG ACAACATGTC CAGTGCGAGC
    GGCCTGGATG TGGGCAAAAT CGAGGAGATG GAGAAGATGC TGAAAGAGGC ACACGCCGAG
    AAGAGCCGGC TCATGGAGTC CAGGGAGCGG GAGATGGAGC TCCGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGAGGCGAGA ACAGGTGGAA CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG
    CAGCAGCTGG TGGAGAAGGA GGTCAAGATG CGAGAGAAAC AGTTTTCCCA GGCACGACCC
    CTGACCCGCT ACCTGCCAAT CCGGAAGGAG GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCGGGCCACG GTGTCGATAC CTGCCTGCAC GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC
    TACTTGGTCA AGATGGGCGG CAAGATTAAA TCATGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTATGTG GACAAGCACG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAAGTGTAC TACGATCACC TGCGCAGTGC AGCCAAGAGC
    CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC
    CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC
    ACTCAGTTCA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_860465.3
    CDS85..4122
    Translation

    Target ORF information:

    RefSeq Version XM_855372.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_855372.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC
    CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG
    AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC
    CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC
    GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG
    GGCTTCCCCC CGCTCATGCA CCACTCCATC CTGCACCACC TGCCGGCTGG CCGGGAGCGC
    GGGGAGGAGG GCGAGCACGC CTACGACACC CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACAGGCGG CAACTCAGCC TGCTCGCCCG ACAACATGTC CAGTGCGAGC
    GGCCTGGATG TGGGCAAAAT CGAGGAGATG GAGAAGATGC TGAAAGAGGC ACACGCCGAG
    AAGAGCCGGC TCATGGAGTC CAGGGAGCGG GAGATGGAGC TCCGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGAGGCGAGA ACAGGTGGAA CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG
    CAGCAGCTGG TGGAGAAGGA GGTCAAGATG CGAGAGAAAC AGTTTTCCCA GGCACGACCC
    CTGACCCGCT ACCTGCCAAT CCGGAAGGAG GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCGGGCCACG GTGTCGATAC CTGCCTGCAC GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC
    TACTTGGTCA AGATGGGCGG CAAGATTAAA TCATGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTATGTG GACAAGCACG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAAGTGTAC TACGATCACC TGCGCAGTGC AGCCAAGAGC
    CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC
    CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC
    ACTCAGTTCA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05888
    Clone ID Related Accession (Same CDS sequence) XM_005619699.2
    Accession Version XM_005619699.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG
    GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC
    CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT
    TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG
    TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC
    TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG
    GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT
    GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA
    AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG
    CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT
    GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG
    CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG
    CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC
    ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC
    ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA
    GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG
    ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG
    CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG
    GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG
    ATGCGAGAGA AACAGTTTTC CCAGGCACGA CCCCTGACCC GCTACCTGCC AATCCGGAAG
    GAGGACTTTG ACCTGAAGAC CCACATCGAG TCGTCGGGCC ACGGTGTCGA TACCTGCCTG
    CACGTGGTGC TTAGCAGCAA GGTCTGCCGC GGCTACTTGG TCAAGATGGG CGGCAAGATT
    AAATCATGGA AGAAGCGCTG GTTTGTCTTC GACCGGCTCA AGCGCACCCT TTCCTATTAT
    GTGGACAAGC ACGAGACGAA GCTGAAGGGG GTCATCTACT TCCAGGCCAT CGAGGAAGTG
    TACTACGATC ACCTGCGCAG TGCAGCCAAG AGGAGGTTTT TCAGCTTTTC TGTGGTGACT
    GAGAGCCCGA ACCCAGCCCT CACCTTCTGT GTGAAGACCC ACGACCGGCT GTATTACATG
    GTGGCCCCGT CTGCAGAGGC CATGCGCATC TGGATGGACG TCATCGTCAC GGGAGCGGAG
    GGCTACACTC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619756.1
    CDS419..5122
    Translation

    Target ORF information:

    RefSeq Version XM_005619699.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619699.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG
    GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC
    CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT
    TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG
    TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC
    TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG
    GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT
    GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA
    AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG
    CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT
    GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG
    CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG
    CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC
    ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC
    ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA
    GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG
    ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG
    CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG
    GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG
    ATGCGAGAGA AACAGTTTTC CCAGGCACGA CCCCTGACCC GCTACCTGCC AATCCGGAAG
    GAGGACTTTG ACCTGAAGAC CCACATCGAG TCGTCGGGCC ACGGTGTCGA TACCTGCCTG
    CACGTGGTGC TTAGCAGCAA GGTCTGCCGC GGCTACTTGG TCAAGATGGG CGGCAAGATT
    AAATCATGGA AGAAGCGCTG GTTTGTCTTC GACCGGCTCA AGCGCACCCT TTCCTATTAT
    GTGGACAAGC ACGAGACGAA GCTGAAGGGG GTCATCTACT TCCAGGCCAT CGAGGAAGTG
    TACTACGATC ACCTGCGCAG TGCAGCCAAG AGGAGGTTTT TCAGCTTTTC TGTGGTGACT
    GAGAGCCCGA ACCCAGCCCT CACCTTCTGT GTGAAGACCC ACGACCGGCT GTATTACATG
    GTGGCCCCGT CTGCAGAGGC CATGCGCATC TGGATGGACG TCATCGTCAC GGGAGCGGAG
    GGCTACACTC AGTTCATGAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05889
    Clone ID Related Accession (Same CDS sequence) XM_005619700.2
    Accession Version XM_005619700.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619700.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGAAT CAGAAAGCCT GGTGAACGGG AACCACACCC CACAGCCTGC CACCCGGGGA
    CCCTCCGCCT GTGCCAGCCA CAGCTCCCTG GTGAGCTCTA TTGAGAAGGA CCTGCAGGAA
    ATCATGGACT CACTGGTGCT AGAGGAGCCT GGGGCGGCAG GCAAGAAGCC TGCTGCGACT
    TCCCCACTGT CGCCGATGGC TAACGGTGGG CGCTACTTGC TGTCCCCGCC GACCAGCCCT
    GGTGCCATGT CCGTGGGCTC CAGCTATGAG AACACCTCTC CAGCCTTCTC TCCACTCTCC
    TCACCAGCCA GCAGTGGAAG CTGTGCCAGC CACTCACCCA GCGGGCAGGA GCCAGCACCT
    TCCATGCCCC CGCTGGTCCC TGCCCGTTCC TCCAGCTACC ATCTGGCTCT GCAGCCCCCA
    CAGCCCCGAC CGAGTGGTGC CCGCCCCTCT GAGAGCCCCC GGTTGGGCAG GAAAGGGGGT
    CACGAGAGGC CTCCCAGTCC TGGCCTCCGA GGTCTGCTGA CAGACAGCCC TGCAGCCACG
    GTCTTGGCTG AGGCCCGCAG AGCCACTGAG AGCCCCCGGC TGGGTGGACA GCTGCCCGTG
    GTGGCCATCA GCCTGAGTGA GTACCCGGCT TCCGGTGCCC GAAGCCAACC CACCAGCATT
    CCTGGCAGCC CCAAGTTCCA GCCTCCAGTC CCCGCTCCCC GAAACAAGAT CGGCACCCTC
    CAGGACCGTC CTCCCAGCCC TTTCCGTGAG CTGCCCAGCA CCGAGCGGGT GCTGACAACC
    AGCCCCTCAC GCCAGCTGGT GGGCCGGACA TTTTCAGATG GGTCAGTCGC CCGCACTCTG
    CAGCCCCCCG AGAGCCCCCG CCTGGGCCGG CGGGGCCTGG ACAGTATGCG GGAATTGCCC
    CCATTGAGCC CGTCACTGTC CCGACGGGCG CTGTCCCCGA TGCCCACCAG GACTACTCCA
    GATCCCAAGC TAACCAGAGA AGTGGCTGAG AGTCCCCGGC CCCGGCGCTG GGCAGCCCAC
    GGGGCATCAC CAGAGGACTT CTCACTGACC CTGGGGGCAC GGGGCCGTAG GACTCGGAGT
    CCCTCACCCA CACTTGGGGA GTCCCTGGCG CCCCGCAAGG GCAGCTTCAG TGGCAGGCTG
    AGCCCGGCCT ACAGTCTGGG CTCTCTGACA GGGGCTTCGC CCCGTCAGAG CCCCCGCGCC
    CAGAGGAAGC TGTCCAGTGG GGACCTGCGG GTACCTGTCA CTCGGGAGCG GAAAAATAGC
    ATTACGGAGA TCAGCGACAA TGAGGACGAC CTCCTGGAGT ACCACCGGCG GCAGCGCCAA
    GAACGGCTCT GGGAGCAGGA GATGGAGAGG CTGGAGCGCC AGCGCCTGGA GACCATACTG
    AACCTGTGTG CCGAGTACAG CCGTGCTGAT GGGGGCCTCG AGGCGGGGGA GCTGCCTAGC
    ATCGGGGAGG CCGCCGCGGC ACTGGCGCTG GCGGGCCGCA GGCCCTCACG AGGCCTTTCT
    GGGGCCCCCG GAGCCTCGGG GCGGAGCACT GAAGAGCCTG GGGGTGCCAC CCCGCGCCTG
    TGGGAGTGTG TGGAGCGCTC AGATGAGGAG AACCTGAAGG AGGAGTGCAG CAGCACGGAG
    AGCACCCAGC AGGAGCACGA AGATGCCCCT AGCACCAAGC TTCAGGGAGA GGTGCTGGCT
    CTGGAAGAGG AGCGGGCTCA GGTGCTGGGG CGTGTGGAGC AGCTCAAAGT CCGCGTGAAG
    GAGCTGGAGC AGCAGCTGCA GGAGTCAGCC CGAGAGGCTG AGATGGAACG GGCGTTGCTG
    CAGGGGGAGA GGGAGGCAGA GCGGGCACTG CTGCAGAAGG AGCAGAAGGC GGTGGACCAG
    CTGCAGGAGA AGTTAGTGAC CTTGGAGACT GGCATCCAGA AGGAGAGGGA CAAGGAGAGG
    GCGGAGCTGG CCGCGGGACG GAGGCACCTG GAGGCCCGCC AGGCGCTCTA CGCCGAGCTC
    CAGACGCAGC TCGATAACTG CCCCGAGTCA GTGCGGGAAC AGTTACAGGA GCAGCTGAGA
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGTACGTG ACGCTTGAGC AGCTAAAGGT GATGTGGGGC
    ACCTCACCCG TGCCCACAGC CCCCGCGCCG GGCCTGCCTT TCTGGGCCTC TGCCTCCCGG
    GACCTGGTTC CCACCACCTG CCTTCCTCCC GTGCTGCCCT CTTCCTCCTC CTCCGCTTCT
    CTCACGCCTT CACCCAAGAT GGAGGAGCTG CTACTCCCTG CTGTAGACTT AGAGCAGTGG
    TACCAGGAGC TGATGGCCGG GCTGGGGACC GGCCCCGCTG CAGCCTCCCC GCGCTCCTCC
    CCCCCGCCTC TGCCCGCCAA AGCTTCCCGG CAGCTGCAGG TTTACCGCTC CAAGATGGAT
    GGTGAGGCCA CCAGCCCCCT GCCCCGGACC CGCAGTGGCC CCCTCCCCTC CTCCTCTGGT
    TCTTCTTCCT CTTCTTCGCA GCTCAGCGTG GCTACTCTGG GCCGGAGCCC CTCCCCAAAG
    AGTGCCCTAC TTGCCCAGAA TGGCACAGGC AGCCTTCCTC GCAACCTGGC GGCCACGCTG
    CAGGACATTG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGGCGAGTC GCTTCCTGCA
    GAGCCCCCCC CAGCAGACGA CCCAGCAGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC
    CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC
    GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC
    TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG
    GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG
    GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA
    CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG
    CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC
    GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA
    TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG
    GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC
    TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG
    AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG
    GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC
    TACACTCAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619757.1
    CDS419..5119
    Translation

    Target ORF information:

    RefSeq Version XM_005619700.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619700.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGAAT CAGAAAGCCT GGTGAACGGG AACCACACCC CACAGCCTGC CACCCGGGGA
    CCCTCCGCCT GTGCCAGCCA CAGCTCCCTG GTGAGCTCTA TTGAGAAGGA CCTGCAGGAA
    ATCATGGACT CACTGGTGCT AGAGGAGCCT GGGGCGGCAG GCAAGAAGCC TGCTGCGACT
    TCCCCACTGT CGCCGATGGC TAACGGTGGG CGCTACTTGC TGTCCCCGCC GACCAGCCCT
    GGTGCCATGT CCGTGGGCTC CAGCTATGAG AACACCTCTC CAGCCTTCTC TCCACTCTCC
    TCACCAGCCA GCAGTGGAAG CTGTGCCAGC CACTCACCCA GCGGGCAGGA GCCAGCACCT
    TCCATGCCCC CGCTGGTCCC TGCCCGTTCC TCCAGCTACC ATCTGGCTCT GCAGCCCCCA
    CAGCCCCGAC CGAGTGGTGC CCGCCCCTCT GAGAGCCCCC GGTTGGGCAG GAAAGGGGGT
    CACGAGAGGC CTCCCAGTCC TGGCCTCCGA GGTCTGCTGA CAGACAGCCC TGCAGCCACG
    GTCTTGGCTG AGGCCCGCAG AGCCACTGAG AGCCCCCGGC TGGGTGGACA GCTGCCCGTG
    GTGGCCATCA GCCTGAGTGA GTACCCGGCT TCCGGTGCCC GAAGCCAACC CACCAGCATT
    CCTGGCAGCC CCAAGTTCCA GCCTCCAGTC CCCGCTCCCC GAAACAAGAT CGGCACCCTC
    CAGGACCGTC CTCCCAGCCC TTTCCGTGAG CTGCCCAGCA CCGAGCGGGT GCTGACAACC
    AGCCCCTCAC GCCAGCTGGT GGGCCGGACA TTTTCAGATG GGTCAGTCGC CCGCACTCTG
    CAGCCCCCCG AGAGCCCCCG CCTGGGCCGG CGGGGCCTGG ACAGTATGCG GGAATTGCCC
    CCATTGAGCC CGTCACTGTC CCGACGGGCG CTGTCCCCGA TGCCCACCAG GACTACTCCA
    GATCCCAAGC TAACCAGAGA AGTGGCTGAG AGTCCCCGGC CCCGGCGCTG GGCAGCCCAC
    GGGGCATCAC CAGAGGACTT CTCACTGACC CTGGGGGCAC GGGGCCGTAG GACTCGGAGT
    CCCTCACCCA CACTTGGGGA GTCCCTGGCG CCCCGCAAGG GCAGCTTCAG TGGCAGGCTG
    AGCCCGGCCT ACAGTCTGGG CTCTCTGACA GGGGCTTCGC CCCGTCAGAG CCCCCGCGCC
    CAGAGGAAGC TGTCCAGTGG GGACCTGCGG GTACCTGTCA CTCGGGAGCG GAAAAATAGC
    ATTACGGAGA TCAGCGACAA TGAGGACGAC CTCCTGGAGT ACCACCGGCG GCAGCGCCAA
    GAACGGCTCT GGGAGCAGGA GATGGAGAGG CTGGAGCGCC AGCGCCTGGA GACCATACTG
    AACCTGTGTG CCGAGTACAG CCGTGCTGAT GGGGGCCTCG AGGCGGGGGA GCTGCCTAGC
    ATCGGGGAGG CCGCCGCGGC ACTGGCGCTG GCGGGCCGCA GGCCCTCACG AGGCCTTTCT
    GGGGCCCCCG GAGCCTCGGG GCGGAGCACT GAAGAGCCTG GGGGTGCCAC CCCGCGCCTG
    TGGGAGTGTG TGGAGCGCTC AGATGAGGAG AACCTGAAGG AGGAGTGCAG CAGCACGGAG
    AGCACCCAGC AGGAGCACGA AGATGCCCCT AGCACCAAGC TTCAGGGAGA GGTGCTGGCT
    CTGGAAGAGG AGCGGGCTCA GGTGCTGGGG CGTGTGGAGC AGCTCAAAGT CCGCGTGAAG
    GAGCTGGAGC AGCAGCTGCA GGAGTCAGCC CGAGAGGCTG AGATGGAACG GGCGTTGCTG
    CAGGGGGAGA GGGAGGCAGA GCGGGCACTG CTGCAGAAGG AGCAGAAGGC GGTGGACCAG
    CTGCAGGAGA AGTTAGTGAC CTTGGAGACT GGCATCCAGA AGGAGAGGGA CAAGGAGAGG
    GCGGAGCTGG CCGCGGGACG GAGGCACCTG GAGGCCCGCC AGGCGCTCTA CGCCGAGCTC
    CAGACGCAGC TCGATAACTG CCCCGAGTCA GTGCGGGAAC AGTTACAGGA GCAGCTGAGA
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGTACGTG ACGCTTGAGC AGCTAAAGGT GATGTGGGGC
    ACCTCACCCG TGCCCACAGC CCCCGCGCCG GGCCTGCCTT TCTGGGCCTC TGCCTCCCGG
    GACCTGGTTC CCACCACCTG CCTTCCTCCC GTGCTGCCCT CTTCCTCCTC CTCCGCTTCT
    CTCACGCCTT CACCCAAGAT GGAGGAGCTG CTACTCCCTG CTGTAGACTT AGAGCAGTGG
    TACCAGGAGC TGATGGCCGG GCTGGGGACC GGCCCCGCTG CAGCCTCCCC GCGCTCCTCC
    CCCCCGCCTC TGCCCGCCAA AGCTTCCCGG CAGCTGCAGG TTTACCGCTC CAAGATGGAT
    GGTGAGGCCA CCAGCCCCCT GCCCCGGACC CGCAGTGGCC CCCTCCCCTC CTCCTCTGGT
    TCTTCTTCCT CTTCTTCGCA GCTCAGCGTG GCTACTCTGG GCCGGAGCCC CTCCCCAAAG
    AGTGCCCTAC TTGCCCAGAA TGGCACAGGC AGCCTTCCTC GCAACCTGGC GGCCACGCTG
    CAGGACATTG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGGCGAGTC GCTTCCTGCA
    GAGCCCCCCC CAGCAGACGA CCCAGCAGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC
    CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC
    GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC
    TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG
    GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG
    GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA
    CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG
    CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC
    GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA
    TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG
    GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC
    TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG
    AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG
    GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC
    TACACTCAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05890
    Clone ID Related Accession (Same CDS sequence) XM_005619701.2
    Accession Version XM_005619701.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619701.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG
    GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC
    CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT
    TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG
    TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC
    TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG
    GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT
    GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA
    AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG
    CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT
    GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG
    CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG
    CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC
    ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC
    ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA
    GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG
    ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG
    CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG
    GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG
    ATGCGAGAGA AACAGTTTTC CCAGGCACGA CCCCTGACCC GCTACCTGCC AATCCGGAAG
    GAGGACTTTG ACCTGAAGAC CCACATCGAG TCGTCGGGCC ACGGTGTCGA TACCTGCCTG
    CACGTGGTGC TTAGCAGCAA GGTCTGCCGC GGCTACTTGG TCAAGATGGG CGGCAAGATT
    AAATCATGGA AGAAGCGCTG GTTTGTCTTC GACCGGCTCA AGCGCACCCT TTCCTATTAT
    GTGGACAAGC ACGAGACGAA GCTGAAGGGG GTCATCTACT TCCAGGCCAT CGAGGAAGTG
    TACTACGATC ACCTGCGCAG TGCAGCCAAG AGCCCGAACC CAGCCCTCAC CTTCTGTGTG
    AAGACCCACG ACCGGCTGTA TTACATGGTG GCCCCGTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TCGTCACGGG AGCGGAGGGC TACACTCAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619758.1
    CDS417..5087
    Translation

    Target ORF information:

    RefSeq Version XM_005619701.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619701.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG
    GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC
    CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT
    TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG
    TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC
    TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG
    GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT
    GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA
    AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG
    CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT
    GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG
    CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG
    CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC
    ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC
    ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA
    GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG
    ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG
    CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG
    GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG
    ATGCGAGAGA AACAGTTTTC CCAGGCACGA CCCCTGACCC GCTACCTGCC AATCCGGAAG
    GAGGACTTTG ACCTGAAGAC CCACATCGAG TCGTCGGGCC ACGGTGTCGA TACCTGCCTG
    CACGTGGTGC TTAGCAGCAA GGTCTGCCGC GGCTACTTGG TCAAGATGGG CGGCAAGATT
    AAATCATGGA AGAAGCGCTG GTTTGTCTTC GACCGGCTCA AGCGCACCCT TTCCTATTAT
    GTGGACAAGC ACGAGACGAA GCTGAAGGGG GTCATCTACT TCCAGGCCAT CGAGGAAGTG
    TACTACGATC ACCTGCGCAG TGCAGCCAAG AGCCCGAACC CAGCCCTCAC CTTCTGTGTG
    AAGACCCACG ACCGGCTGTA TTACATGGTG GCCCCGTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TCGTCACGGG AGCGGAGGGC TACACTCAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05891
    Clone ID Related Accession (Same CDS sequence) XM_005619702.2
    Accession Version XM_005619702.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4659bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG
    GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC
    CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT
    TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG
    TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC
    TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG
    GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT
    GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA
    AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG
    CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGACA GCAGGTGATC
    GAGGAGCAGC GGCGGCGCCT GGCCGAGCTG AAGCAGAAGG CTGCGGCCGA GGCGCAGTGC
    CAGTGGGACG CCCTGCACGG GGCGGCCCCC TTCCCCCCGG GCCCCTCGGG CTTCCCCCCG
    CTCATGCACC ACTCCATCCT GCACCACCTG CCGGCTGGCC GGGAGCGCGG GGAGGAGGGC
    GAGCACGCCT ACGACACCCT GAGCCTGGAG AGCTCCGACA GCATGGAGAC CAGCATCTCC
    ACAGGCGGCA ACTCAGCCTG CTCGCCCGAC AACATGTCCA GTGCGAGCGG CCTGGATGTG
    GGCAAAATCG AGGAGATGGA GAAGATGCTG AAAGAGGCAC ACGCCGAGAA GAGCCGGCTC
    ATGGAGTCCA GGGAGCGGGA GATGGAGCTC CGGCGGCAGG CCCTGGAGGA GGAACGGAGG
    AGGCGAGAAC AGGTGGAACG GAGGCTACAG AGCGAGAGCG CCAGGAGGCA GCAGCTGGTG
    GAGAAGGAGG TCAAGATGCG AGAGAAACAG TTTTCCCAGG CACGACCCCT GACCCGCTAC
    CTGCCAATCC GGAAGGAGGA CTTTGACCTG AAGACCCACA TCGAGTCGTC GGGCCACGGT
    GTCGATACCT GCCTGCACGT GGTGCTTAGC AGCAAGGTCT GCCGCGGCTA CTTGGTCAAG
    ATGGGCGGCA AGATTAAATC ATGGAAGAAG CGCTGGTTTG TCTTCGACCG GCTCAAGCGC
    ACCCTTTCCT ATTATGTGGA CAAGCACGAG ACGAAGCTGA AGGGGGTCAT CTACTTCCAG
    GCCATCGAGG AAGTGTACTA CGATCACCTG CGCAGTGCAG CCAAGAGGAG GTTTTTCAGC
    TTTTCTGTGG TGACTGAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC
    CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC
    GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619759.1
    CDS418..5076
    Translation

    Target ORF information:

    RefSeq Version XM_005619702.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619702.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG
    GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC
    CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT
    TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG
    TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC
    TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG
    GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT
    GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA
    AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG
    CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGACA GCAGGTGATC
    GAGGAGCAGC GGCGGCGCCT GGCCGAGCTG AAGCAGAAGG CTGCGGCCGA GGCGCAGTGC
    CAGTGGGACG CCCTGCACGG GGCGGCCCCC TTCCCCCCGG GCCCCTCGGG CTTCCCCCCG
    CTCATGCACC ACTCCATCCT GCACCACCTG CCGGCTGGCC GGGAGCGCGG GGAGGAGGGC
    GAGCACGCCT ACGACACCCT GAGCCTGGAG AGCTCCGACA GCATGGAGAC CAGCATCTCC
    ACAGGCGGCA ACTCAGCCTG CTCGCCCGAC AACATGTCCA GTGCGAGCGG CCTGGATGTG
    GGCAAAATCG AGGAGATGGA GAAGATGCTG AAAGAGGCAC ACGCCGAGAA GAGCCGGCTC
    ATGGAGTCCA GGGAGCGGGA GATGGAGCTC CGGCGGCAGG CCCTGGAGGA GGAACGGAGG
    AGGCGAGAAC AGGTGGAACG GAGGCTACAG AGCGAGAGCG CCAGGAGGCA GCAGCTGGTG
    GAGAAGGAGG TCAAGATGCG AGAGAAACAG TTTTCCCAGG CACGACCCCT GACCCGCTAC
    CTGCCAATCC GGAAGGAGGA CTTTGACCTG AAGACCCACA TCGAGTCGTC GGGCCACGGT
    GTCGATACCT GCCTGCACGT GGTGCTTAGC AGCAAGGTCT GCCGCGGCTA CTTGGTCAAG
    ATGGGCGGCA AGATTAAATC ATGGAAGAAG CGCTGGTTTG TCTTCGACCG GCTCAAGCGC
    ACCCTTTCCT ATTATGTGGA CAAGCACGAG ACGAAGCTGA AGGGGGTCAT CTACTTCCAG
    GCCATCGAGG AAGTGTACTA CGATCACCTG CGCAGTGCAG CCAAGAGGAG GTTTTTCAGC
    TTTTCTGTGG TGACTGAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC
    CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC
    GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05892
    Clone ID Related Accession (Same CDS sequence) XM_005619703.2
    Accession Version XM_005619703.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGATGCTC TTAATAGGAA CCAGGTAGGC CCTGGATGCA AGACCCAAGC AATGGTAAAG 
    AAAGGGCCCT TGGACCTGAT CGAGACAGGC AAAGGGCTGA AGGTGCAAAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG TGGGCGGCTG AGCACGGCTA TCACCCTTCT GCCTCTGGAG
    GAAGGGAAGA CGGTGATTGG CTCCGCAGCC AGGGACATCT CCCTGCAGGG CCCAGGCTTG
    GCTCCGGAGC ACTGCTACAT CGAGAACGTG CGGGGCACCC TCACCCTCTA CCCTTGTGGC
    AATGCCTGCA GTATTGACGG GCTCCCCATC CGGCAGCCCA CCAGGCTCAC TCAGGGCTGT
    ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA
    GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC
    TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC
    ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC
    CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT
    GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA
    CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC
    ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG
    CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC
    GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC
    TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG
    GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT
    GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG
    GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC
    CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG
    CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA
    TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT
    CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG
    GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC
    TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC
    CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG
    AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT
    ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA
    CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC
    CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC
    GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG
    GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG
    GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC
    ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG
    GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG
    CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG
    GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG
    CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG
    GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG
    ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG
    GAGGCCGAGG CCCTGGAGAC GGAGACAAAG CTGTTTGAGG ACTTGGAGTT CCAGCAGCTG
    GAGCGGGAGA GCCGCGTGGA GGAGGAGCGG GAGCTGGCAG GCCAGGGGCT GCTCCGGAGC
    CAGGCCGAGC TGCTCCGCAG CATCACCAAG AGGAAGGAGC GCCTGGCAGT CCTGGACAGT
    CAGGCGGGGC AGATCCGGGC CCAGGCCGTG CAGGAGTCCG AGCGCCTGGC GAGGGACAAG
    AACGCTTCCC TGCAGCTGCT GCAGAAGGAG AAGGAGAGAC TGACTATGTT GGAGGGAAGA
    TACCACTCAC TCACAGGAGG CAGGCCCTTC CCGAAGACCA CGTCCACCCT CAAAGAGGCT
    GAACTGCTCA TCTCCGAGTC CTCAGAGATG GGGCTGGGGT CGGCGACTCT GGTCCCTTTC
    CCAGGGTCTT CTCAGCCCGG GGCCTTCTCT GTCCCCCTAA CCCAACCTGC TTCCACTCAG
    CTCTGCCCCA AAGCACAAGA GTACGTGACG CTTGAGCAGC TAAAGGTGAT GTGGGGCACC
    TCACCCGTGC CCACAGCCCC CGCGCCGGGC CTGCCTTTCT GGGCCTCTGC CTCCCGGGAC
    CTGGTTCCCA CCACCTGCCT TCCTCCCGTG CTGCCCTCTT CCTCCTCCTC CGCTTCTCTC
    ACGCCTTCAC CCAAGATGGA GGAGCTGCTA CTCCCTGCTG TAGACTTAGA GCAGTGGTAC
    CAGGAGCTGA TGGCCGGGCT GGGGACCGGC CCCGCTGCAG CCTCCCCGCG CTCCTCCCCC
    CCGCCTCTGC CCGCCAAAGC TTCCCGGCAG CTGCAGGTTT ACCGCTCCAA GATGGATGGT
    GAGGCCACCA GCCCCCTGCC CCGGACCCGC AGTGGCCCCC TCCCCTCCTC CTCTGGTTCT
    TCTTCCTCTT CTTCGCAGCT CAGCGTGGCT ACTCTGGGCC GGAGCCCCTC CCCAAAGAGT
    GCCCTACTTG CCCAGAATGG CACAGGCAGC CTTCCTCGCA ACCTGGCGGC CACGCTGCAG
    GACATTGAGA CCAAGCGCCA GCTGGCCCTG CAGCAGAAGG GCGAGTCGCT TCCTGCAGAG
    CCCCCCCCAG CAGACGACCC AGCAGGACAG CAGGTGATCG AGGAGCAGCG GCGGCGCCTG
    GCCGAGCTGA AGCAGAAGGC TGCGGCCGAG GCGCAGTGCC AGTGGGACGC CCTGCACGGG
    GCGGCCCCCT TCCCCCCGGG CCCCTCGGGC TTCCCCCCGC TCATGCACCA CTCCATCCTG
    CACCACCTGC CGGCTGGCCG GGAGCGCGGG GAGGAGGGCG AGCACGCCTA CGACACCCTG
    AGCCTGGAGA GCTCCGACAG CATGGAGACC AGCATCTCCA CAGGCGGCAA CTCAGCCTGC
    TCGCCCGACA ACATGTCCAG TGCGAGCGGC CTGGATGTGG GCAAAATCGA GGAGATGGAG
    AAGATGCTGA AAGAGGCACA CGCCGAGAAG AGCCGGCTCA TGGAGTCCAG GGAGCGGGAG
    ATGGAGCTCC GGCGGCAGGC CCTGGAGGAG GAACGGAGGA GGCGAGAACA GGTGGAACGG
    AGGCTACAGA GCGAGAGCGC CAGGAGGCAG CAGCTGGTGG AGAAGGAGGT CAAGATGCGA
    GAGAAACAGT TTTCCCAGGC ACGACCCCTG ACCCGCTACC TGCCAATCCG GAAGGAGGAC
    TTTGACCTGA AGACCCACAT CGAGTCGTCG GGCCACGGTG TCGATACCTG CCTGCACGTG
    GTGCTTAGCA GCAAGGTCTG CCGCGGCTAC TTGGTCAAGA TGGGCGGCAA GATTAAATCA
    TGGAAGAAGC GCTGGTTTGT CTTCGACCGG CTCAAGCGCA CCCTTTCCTA TTATGTGGAC
    AAGCACGAGA CGAAGCTGAA GGGGGTCATC TACTTCCAGG CCATCGAGGA AGTGTACTAC
    GATCACCTGC GCAGTGCAGC CAAGAGGAGG TTTTTCAGCT TTTCTGTGGT GACTGAGAGC
    CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC
    CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC
    ACTCAGTTCA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619760.1
    CDS324..4961
    Translation

    Target ORF information:

    RefSeq Version XM_005619703.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619703.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGATGCTC TTAATAGGAA CCAGGTAGGC CCTGGATGCA AGACCCAAGC AATGGTAAAG 
    AAAGGGCCCT TGGACCTGAT CGAGACAGGC AAAGGGCTGA AGGTGCAAAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG TGGGCGGCTG AGCACGGCTA TCACCCTTCT GCCTCTGGAG
    GAAGGGAAGA CGGTGATTGG CTCCGCAGCC AGGGACATCT CCCTGCAGGG CCCAGGCTTG
    GCTCCGGAGC ACTGCTACAT CGAGAACGTG CGGGGCACCC TCACCCTCTA CCCTTGTGGC
    AATGCCTGCA GTATTGACGG GCTCCCCATC CGGCAGCCCA CCAGGCTCAC TCAGGGCTGT
    ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA
    GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC
    TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC
    ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC
    CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT
    GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA
    CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC
    ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG
    CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC
    GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC
    TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG
    GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT
    GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG
    GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC
    CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG
    CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA
    TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT
    CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG
    GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC
    TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC
    CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG
    AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT
    ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA
    CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC
    CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC
    GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG
    GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG
    GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC
    ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG
    GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG
    CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG
    GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG
    CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG
    GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG
    ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG
    GAGGCCGAGG CCCTGGAGAC GGAGACAAAG CTGTTTGAGG ACTTGGAGTT CCAGCAGCTG
    GAGCGGGAGA GCCGCGTGGA GGAGGAGCGG GAGCTGGCAG GCCAGGGGCT GCTCCGGAGC
    CAGGCCGAGC TGCTCCGCAG CATCACCAAG AGGAAGGAGC GCCTGGCAGT CCTGGACAGT
    CAGGCGGGGC AGATCCGGGC CCAGGCCGTG CAGGAGTCCG AGCGCCTGGC GAGGGACAAG
    AACGCTTCCC TGCAGCTGCT GCAGAAGGAG AAGGAGAGAC TGACTATGTT GGAGGGAAGA
    TACCACTCAC TCACAGGAGG CAGGCCCTTC CCGAAGACCA CGTCCACCCT CAAAGAGGCT
    GAACTGCTCA TCTCCGAGTC CTCAGAGATG GGGCTGGGGT CGGCGACTCT GGTCCCTTTC
    CCAGGGTCTT CTCAGCCCGG GGCCTTCTCT GTCCCCCTAA CCCAACCTGC TTCCACTCAG
    CTCTGCCCCA AAGCACAAGA GTACGTGACG CTTGAGCAGC TAAAGGTGAT GTGGGGCACC
    TCACCCGTGC CCACAGCCCC CGCGCCGGGC CTGCCTTTCT GGGCCTCTGC CTCCCGGGAC
    CTGGTTCCCA CCACCTGCCT TCCTCCCGTG CTGCCCTCTT CCTCCTCCTC CGCTTCTCTC
    ACGCCTTCAC CCAAGATGGA GGAGCTGCTA CTCCCTGCTG TAGACTTAGA GCAGTGGTAC
    CAGGAGCTGA TGGCCGGGCT GGGGACCGGC CCCGCTGCAG CCTCCCCGCG CTCCTCCCCC
    CCGCCTCTGC CCGCCAAAGC TTCCCGGCAG CTGCAGGTTT ACCGCTCCAA GATGGATGGT
    GAGGCCACCA GCCCCCTGCC CCGGACCCGC AGTGGCCCCC TCCCCTCCTC CTCTGGTTCT
    TCTTCCTCTT CTTCGCAGCT CAGCGTGGCT ACTCTGGGCC GGAGCCCCTC CCCAAAGAGT
    GCCCTACTTG CCCAGAATGG CACAGGCAGC CTTCCTCGCA ACCTGGCGGC CACGCTGCAG
    GACATTGAGA CCAAGCGCCA GCTGGCCCTG CAGCAGAAGG GCGAGTCGCT TCCTGCAGAG
    CCCCCCCCAG CAGACGACCC AGCAGGACAG CAGGTGATCG AGGAGCAGCG GCGGCGCCTG
    GCCGAGCTGA AGCAGAAGGC TGCGGCCGAG GCGCAGTGCC AGTGGGACGC CCTGCACGGG
    GCGGCCCCCT TCCCCCCGGG CCCCTCGGGC TTCCCCCCGC TCATGCACCA CTCCATCCTG
    CACCACCTGC CGGCTGGCCG GGAGCGCGGG GAGGAGGGCG AGCACGCCTA CGACACCCTG
    AGCCTGGAGA GCTCCGACAG CATGGAGACC AGCATCTCCA CAGGCGGCAA CTCAGCCTGC
    TCGCCCGACA ACATGTCCAG TGCGAGCGGC CTGGATGTGG GCAAAATCGA GGAGATGGAG
    AAGATGCTGA AAGAGGCACA CGCCGAGAAG AGCCGGCTCA TGGAGTCCAG GGAGCGGGAG
    ATGGAGCTCC GGCGGCAGGC CCTGGAGGAG GAACGGAGGA GGCGAGAACA GGTGGAACGG
    AGGCTACAGA GCGAGAGCGC CAGGAGGCAG CAGCTGGTGG AGAAGGAGGT CAAGATGCGA
    GAGAAACAGT TTTCCCAGGC ACGACCCCTG ACCCGCTACC TGCCAATCCG GAAGGAGGAC
    TTTGACCTGA AGACCCACAT CGAGTCGTCG GGCCACGGTG TCGATACCTG CCTGCACGTG
    GTGCTTAGCA GCAAGGTCTG CCGCGGCTAC TTGGTCAAGA TGGGCGGCAA GATTAAATCA
    TGGAAGAAGC GCTGGTTTGT CTTCGACCGG CTCAAGCGCA CCCTTTCCTA TTATGTGGAC
    AAGCACGAGA CGAAGCTGAA GGGGGTCATC TACTTCCAGG CCATCGAGGA AGTGTACTAC
    GATCACCTGC GCAGTGCAGC CAAGAGGAGG TTTTTCAGCT TTTCTGTGGT GACTGAGAGC
    CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC
    CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC
    ACTCAGTTCA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05893
    Clone ID Related Accession (Same CDS sequence) XM_005619704.2
    Accession Version XM_005619704.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA
    CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA
    GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC
    TGCCCCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCA
    CCCGTGCCCA CAGCCCCCGC GCCGGGCCTG CCTTTCTGGG CCTCTGCCTC CCGGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGTGCTG CCCTCTTCCT CCTCCTCCGC TTCTCTCACG
    CCTTCACCCA AGATGGAGGA GCTGCTACTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACCGGCCCC GCTGCAGCCT CCCCGCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGGCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGTGAG
    GCCACCAGCC CCCTGCCCCG GACCCGCAGT GGCCCCCTCC CCTCCTCCTC TGGTTCTTCT
    TCCTCTTCTT CGCAGCTCAG CGTGGCTACT CTGGGCCGGA GCCCCTCCCC AAAGAGTGCC
    CTACTTGCCC AGAATGGCAC AGGCAGCCTT CCTCGCAACC TGGCGGCCAC GCTGCAGGAC
    ATTGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGCG AGTCGCTTCC TGCAGAGCCC
    CCCCCAGCAG ACGACCCAGC AGGACAGCAG GTGATCGAGG AGCAGCGGCG GCGCCTGGCC
    GAGCTGAAGC AGAAGGCTGC GGCCGAGGCG CAGTGCCAGT GGGACGCCCT GCACGGGGCG
    GCCCCCTTCC CCCCGGGCCC CTCGGGCTTC CCCCCGCTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CTGGCCGGGA GCGCGGGGAG GAGGGCGAGC ACGCCTACGA CACCCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACAG GCGGCAACTC AGCCTGCTCG
    CCCGACAACA TGTCCAGTGC GAGCGGCCTG GATGTGGGCA AAATCGAGGA GATGGAGAAG
    ATGCTGAAAG AGGCACACGC CGAGAAGAGC CGGCTCATGG AGTCCAGGGA GCGGGAGATG
    GAGCTCCGGC GGCAGGCCCT GGAGGAGGAA CGGAGGAGGC GAGAACAGGT GGAACGGAGG
    CTACAGAGCG AGAGCGCCAG GAGGCAGCAG CTGGTGGAGA AGGAGGTCAA GATGCGAGAG
    AAACAGTTTT CCCAGGCACG ACCCCTGACC CGCTACCTGC CAATCCGGAA GGAGGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCGGGC CACGGTGTCG ATACCTGCCT GCACGTGGTG
    CTTAGCAGCA AGGTCTGCCG CGGCTACTTG GTCAAGATGG GCGGCAAGAT TAAATCATGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA TGTGGACAAG
    CACGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAAGT GTACTACGAT
    CACCTGCGCA GTGCAGCCAA GAGGAGGTTT TTCAGCTTTT CTGTGGTGAC TGAGAGCCCG
    AACCCAGCCC TCACCTTCTG TGTGAAGACC CACGACCGGC TGTATTACAT GGTGGCCCCG
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATCGTCA CGGGAGCGGA GGGCTACACT
    CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619761.1
    CDS417..4991
    Translation

    Target ORF information:

    RefSeq Version XM_005619704.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619704.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA
    CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA
    GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC
    TGCCCCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCA
    CCCGTGCCCA CAGCCCCCGC GCCGGGCCTG CCTTTCTGGG CCTCTGCCTC CCGGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGTGCTG CCCTCTTCCT CCTCCTCCGC TTCTCTCACG
    CCTTCACCCA AGATGGAGGA GCTGCTACTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACCGGCCCC GCTGCAGCCT CCCCGCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGGCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGTGAG
    GCCACCAGCC CCCTGCCCCG GACCCGCAGT GGCCCCCTCC CCTCCTCCTC TGGTTCTTCT
    TCCTCTTCTT CGCAGCTCAG CGTGGCTACT CTGGGCCGGA GCCCCTCCCC AAAGAGTGCC
    CTACTTGCCC AGAATGGCAC AGGCAGCCTT CCTCGCAACC TGGCGGCCAC GCTGCAGGAC
    ATTGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGCG AGTCGCTTCC TGCAGAGCCC
    CCCCCAGCAG ACGACCCAGC AGGACAGCAG GTGATCGAGG AGCAGCGGCG GCGCCTGGCC
    GAGCTGAAGC AGAAGGCTGC GGCCGAGGCG CAGTGCCAGT GGGACGCCCT GCACGGGGCG
    GCCCCCTTCC CCCCGGGCCC CTCGGGCTTC CCCCCGCTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CTGGCCGGGA GCGCGGGGAG GAGGGCGAGC ACGCCTACGA CACCCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACAG GCGGCAACTC AGCCTGCTCG
    CCCGACAACA TGTCCAGTGC GAGCGGCCTG GATGTGGGCA AAATCGAGGA GATGGAGAAG
    ATGCTGAAAG AGGCACACGC CGAGAAGAGC CGGCTCATGG AGTCCAGGGA GCGGGAGATG
    GAGCTCCGGC GGCAGGCCCT GGAGGAGGAA CGGAGGAGGC GAGAACAGGT GGAACGGAGG
    CTACAGAGCG AGAGCGCCAG GAGGCAGCAG CTGGTGGAGA AGGAGGTCAA GATGCGAGAG
    AAACAGTTTT CCCAGGCACG ACCCCTGACC CGCTACCTGC CAATCCGGAA GGAGGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCGGGC CACGGTGTCG ATACCTGCCT GCACGTGGTG
    CTTAGCAGCA AGGTCTGCCG CGGCTACTTG GTCAAGATGG GCGGCAAGAT TAAATCATGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA TGTGGACAAG
    CACGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAAGT GTACTACGAT
    CACCTGCGCA GTGCAGCCAA GAGGAGGTTT TTCAGCTTTT CTGTGGTGAC TGAGAGCCCG
    AACCCAGCCC TCACCTTCTG TGTGAAGACC CACGACCGGC TGTATTACAT GGTGGCCCCG
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATCGTCA CGGGAGCGGA GGGCTACACT
    CAGTTCATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05894
    Clone ID Related Accession (Same CDS sequence) XM_005619705.2
    Accession Version XM_005619705.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619705.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGATG GAGGAGCTGC TACTCCCTGC TGTAGACTTA
    GAGCAGTGGT ACCAGGAGCT GATGGCCGGG CTGGGGACCG GCCCCGCTGC AGCCTCCCCG
    CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA GCTTCCCGGC AGCTGCAGGT TTACCGCTCC
    AAGATGGATG GTGAGGCCAC CAGCCCCCTG CCCCGGACCC GCAGTGGCCC CCTCCCCTCC
    TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG CTCAGCGTGG CTACTCTGGG CCGGAGCCCC
    TCCCCAAAGA GTGCCCTACT TGCCCAGAAT GGCACAGGCA GCCTTCCTCG CAACCTGGCG
    GCCACGCTGC AGGACATTGA GACCAAGCGC CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG
    CTTCCTGCAG AGCCCCCCCC AGCAGACGAC CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG
    CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC
    GCCCTGCACG GGGCGGCCCC CTTCCCCCCG GGCCCCTCGG GCTTCCCCCC GCTCATGCAC
    CACTCCATCC TGCACCACCT GCCGGCTGGC CGGGAGCGCG GGGAGGAGGG CGAGCACGCC
    TACGACACCC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACAGGCGGC
    AACTCAGCCT GCTCGCCCGA CAACATGTCC AGTGCGAGCG GCCTGGATGT GGGCAAAATC
    GAGGAGATGG AGAAGATGCT GAAAGAGGCA CACGCCGAGA AGAGCCGGCT CATGGAGTCC
    AGGGAGCGGG AGATGGAGCT CCGGCGGCAG GCCCTGGAGG AGGAACGGAG GAGGCGAGAA
    CAGGTGGAAC GGAGGCTACA GAGCGAGAGC GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG
    GTCAAGATGC GAGAGAAACA GTTTTCCCAG GCACGACCCC TGACCCGCTA CCTGCCAATC
    CGGAAGGAGG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CGGGCCACGG TGTCGATACC
    TGCCTGCACG TGGTGCTTAG CAGCAAGGTC TGCCGCGGCT ACTTGGTCAA GATGGGCGGC
    AAGATTAAAT CATGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC
    TATTATGTGG ACAAGCACGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG
    GAAGTGTACT ACGATCACCT GCGCAGTGCA GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG
    GTGACTGAGA GCCCGAACCC AGCCCTCACC TTCTGTGTGA AGACCCACGA CCGGCTGTAT
    TACATGGTGG CCCCGTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT CGTCACGGGA
    GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619762.1
    CDS417..4946
    Translation

    Target ORF information:

    RefSeq Version XM_005619705.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619705.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGATG GAGGAGCTGC TACTCCCTGC TGTAGACTTA
    GAGCAGTGGT ACCAGGAGCT GATGGCCGGG CTGGGGACCG GCCCCGCTGC AGCCTCCCCG
    CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA GCTTCCCGGC AGCTGCAGGT TTACCGCTCC
    AAGATGGATG GTGAGGCCAC CAGCCCCCTG CCCCGGACCC GCAGTGGCCC CCTCCCCTCC
    TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG CTCAGCGTGG CTACTCTGGG CCGGAGCCCC
    TCCCCAAAGA GTGCCCTACT TGCCCAGAAT GGCACAGGCA GCCTTCCTCG CAACCTGGCG
    GCCACGCTGC AGGACATTGA GACCAAGCGC CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG
    CTTCCTGCAG AGCCCCCCCC AGCAGACGAC CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG
    CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC
    GCCCTGCACG GGGCGGCCCC CTTCCCCCCG GGCCCCTCGG GCTTCCCCCC GCTCATGCAC
    CACTCCATCC TGCACCACCT GCCGGCTGGC CGGGAGCGCG GGGAGGAGGG CGAGCACGCC
    TACGACACCC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACAGGCGGC
    AACTCAGCCT GCTCGCCCGA CAACATGTCC AGTGCGAGCG GCCTGGATGT GGGCAAAATC
    GAGGAGATGG AGAAGATGCT GAAAGAGGCA CACGCCGAGA AGAGCCGGCT CATGGAGTCC
    AGGGAGCGGG AGATGGAGCT CCGGCGGCAG GCCCTGGAGG AGGAACGGAG GAGGCGAGAA
    CAGGTGGAAC GGAGGCTACA GAGCGAGAGC GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG
    GTCAAGATGC GAGAGAAACA GTTTTCCCAG GCACGACCCC TGACCCGCTA CCTGCCAATC
    CGGAAGGAGG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CGGGCCACGG TGTCGATACC
    TGCCTGCACG TGGTGCTTAG CAGCAAGGTC TGCCGCGGCT ACTTGGTCAA GATGGGCGGC
    AAGATTAAAT CATGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC
    TATTATGTGG ACAAGCACGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG
    GAAGTGTACT ACGATCACCT GCGCAGTGCA GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG
    GTGACTGAGA GCCCGAACCC AGCCCTCACC TTCTGTGTGA AGACCCACGA CCGGCTGTAT
    TACATGGTGG CCCCGTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT CGTCACGGGA
    GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05895
    Clone ID Related Accession (Same CDS sequence) XM_005619706.2
    Accession Version XM_005619706.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA
    GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG
    CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC
    CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC
    ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA
    GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC
    GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT
    TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC
    AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC
    AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT TCTGTGGTGA CTGAGAGCCC GAACCCAGCC
    CTCACCTTCT GTGTGAAGAC CCACGACCGG CTGTATTACA TGGTGGCCCC GTCTGCAGAG
    GCCATGCGCA TCTGGATGGA CGTCATCGTC ACGGGAGCGG AGGGCTACAC TCAGTTCATG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619763.1
    CDS414..4799
    Translation

    Target ORF information:

    RefSeq Version XM_005619706.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619706.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA
    GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG
    CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC
    CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC
    ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA
    GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC
    GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT
    TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC
    AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC
    AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT TCTGTGGTGA CTGAGAGCCC GAACCCAGCC
    CTCACCTTCT GTGTGAAGAC CCACGACCGG CTGTATTACA TGGTGGCCCC GTCTGCAGAG
    GCCATGCGCA TCTGGATGGA CGTCATCGTC ACGGGAGCGG AGGGCTACAC TCAGTTCATG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05897
    Clone ID Related Accession (Same CDS sequence) XM_546499.4 , XM_546499.5
    Accession Version XM_546499.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_546499.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG
    CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC
    CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC
    GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG
    CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG
    CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA
    CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA
    CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT
    AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC
    CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC AGCTTTTCTG TGGTGACTGA GAGCCCGAAC
    CCAGCCCTCA CCTTCTGTGT GAAGACCCAC GACCGGCTGT ATTACATGGT GGCCCCGTCT
    GCAGAGGCCA TGCGCATCTG GATGGACGTC ATCGTCACGG GAGCGGAGGG CTACACTCAG
    TTCATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546499.3
    CDS410..4621
    Translation

    Target ORF information:

    RefSeq Version XM_546499.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546499.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG
    CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC
    CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC
    GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG
    CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG
    CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA
    CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA
    CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT
    AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC
    CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC AGCTTTTCTG TGGTGACTGA GAGCCCGAAC
    CCAGCCCTCA CCTTCTGTGT GAAGACCCAC GACCGGCTGT ATTACATGGT GGCCCCGTCT
    GCAGAGGCCA TGCGCATCTG GATGGACGTC ATCGTCACGG GAGCGGAGGG CTACACTCAG
    TTCATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05897
    Clone ID Related Accession (Same CDS sequence) XM_546499.4 , XM_546499.5
    Accession Version XM_546499.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_546499.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG
    CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC
    CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC
    GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG
    CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG
    CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA
    CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA
    CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT
    AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC
    CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC AGCTTTTCTG TGGTGACTGA GAGCCCGAAC
    CCAGCCCTCA CCTTCTGTGT GAAGACCCAC GACCGGCTGT ATTACATGGT GGCCCCGTCT
    GCAGAGGCCA TGCGCATCTG GATGGACGTC ATCGTCACGG GAGCGGAGGG CTACACTCAG
    TTCATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546499.3
    CDS87..4298
    Translation

    Target ORF information:

    RefSeq Version XM_546499.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546499.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG
    CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC
    CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC
    GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG
    CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG
    CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA
    CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA
    CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT
    AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC
    CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC AGCTTTTCTG TGGTGACTGA GAGCCCGAAC
    CCAGCCCTCA CCTTCTGTGT GAAGACCCAC GACCGGCTGT ATTACATGGT GGCCCCGTCT
    GCAGAGGCCA TGCGCATCTG GATGGACGTC ATCGTCACGG GAGCGGAGGG CTACACTCAG
    TTCATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05898
    Clone ID Related Accession (Same CDS sequence) XM_005619708.3 , XM_005619708.2
    Accession Version XM_005619708.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG
    CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC
    CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC
    GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG
    CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG
    CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA
    CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA
    CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT
    AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC
    CTGCGCAGTG CAGCCAAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC
    CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC
    GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619765.1
    CDS86..4264
    Translation

    Target ORF information:

    RefSeq Version XM_005619708.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619708.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG
    CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC
    CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC
    GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG
    CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG
    CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA
    CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA
    CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT
    AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC
    CTGCGCAGTG CAGCCAAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC
    CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC
    GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05898
    Clone ID Related Accession (Same CDS sequence) XM_005619708.3 , XM_005619708.2
    Accession Version XM_005619708.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619708.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG
    CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC
    CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC
    GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG
    CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG
    CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA
    CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA
    CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT
    AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC
    CTGCGCAGTG CAGCCAAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC
    CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC
    GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619765.1
    CDS409..4587
    Translation

    Target ORF information:

    RefSeq Version XM_005619708.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619708.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG
    CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC
    CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC
    GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG
    CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG
    CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA
    CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA
    CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT
    AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC
    CTGCGCAGTG CAGCCAAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC
    CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC
    GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287933
    Clone ID Related Accession (Same CDS sequence) XM_022418225.1
    Accession Version XM_022418225.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC
    TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC
    GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC
    ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC
    AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA
    GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG
    CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC
    CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC
    ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA
    GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC
    GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT
    TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC
    AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC
    AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT TCTGTGGTGA CTGAGAGCCC GAACCCAGCC
    CTCACCTTCT GTGTGAAGAC CCACGACCGG CTGTATTACA TGGTGGCCCC GTCTGCAGAG
    GCCATGCGCA TCTGGATGGA CGTCATCGTC ACGGGAGCGG AGGGCTACAC TCAGTTCATG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273933.1
    CDS364..5289
    Translation

    Target ORF information:

    RefSeq Version XM_022418225.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418225.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC
    TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC
    GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC
    ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC
    AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA
    GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG
    CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC
    CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC
    ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA
    GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC
    GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT
    TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC
    AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC
    AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT TCTGTGGTGA CTGAGAGCCC GAACCCAGCC
    CTCACCTTCT GTGTGAAGAC CCACGACCGG CTGTATTACA TGGTGGCCCC GTCTGCAGAG
    GCCATGCGCA TCTGGATGGA CGTCATCGTC ACGGGAGCGG AGGGCTACAC TCAGTTCATG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287934
    Clone ID Related Accession (Same CDS sequence) XM_022418226.1
    Accession Version XM_022418226.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGAAT CAGAAAGCCT GGTGAACGGG AACCACACCC CACAGCCTGC CACCCGGGGA
    CCCTCCGCCT GTGCCAGCCA CAGCTCCCTG GTGAGCTCTA TTGAGAAGGA CCTGCAGGAA
    ATCATGGACT CACTGGTGCT AGAGGAGCCT GGGGCGGCAG GCAAGAAGCC TGCTGCGACT
    TCCCCACTGT CGCCGATGGC TAACGGTGGG CGCTACTTGC TGTCCCCGCC GACCAGCCCT
    GGTGCCATGT CCGTGGGCTC CAGCTATGAG AACACCTCTC CAGCCTTCTC TCCACTCTCC
    TCACCAGCCA GCAGTGGAAG CTGTGCCAGC CACTCACCCA GCGGGCAGGA GCCAGCACCT
    TCCATGCCCC CGCTGGTCCC TGCCCGTTCC TCCAGCTACC ATCTGGCTCT GCAGCCCCCA
    CAGCCCCGAC CGAGTGGTGC CCGCCCCTCT GAGAGCCCCC GGTTGGGCAG GAAAGGGGGT
    CACGAGAGGC CTCCCAGTCC TGGCCTCCGA GGTCTGCTGA CAGACAGCCC TGCAGCCACG
    GTCTTGGCTG AGGCCCGCAG AGCCACTGAG AGCCCCCGGC TGGGTGGACA GCTGCCCGTG
    GTGGCCATCA GCCTGAGTGA GTACCCGGCT TCCGGTGCCC GAAGCCAACC CACCAGCATT
    CCTGGCAGCC CCAAGTTCCA GCCTCCAGTC CCCGCTCCCC GAAACAAGAT CGGCACCCTC
    CAGGACCGTC CTCCCAGCCC TTTCCGTGAG CTGCCCAGCA CCGAGCGGGT GCTGACAACC
    AGCCCCTCAC GCCAGCTGGT GGGCCGGACA TTTTCAGATG GGTCAGTCGC CCGCACTCTG
    CAGCCCCCCG AGAGCCCCCG CCTGGGCCGG CGGGGCCTGG ACAGTATGCG GGAATTGCCC
    CCATTGAGCC CGTCACTGTC CCGACGGGCG CTGTCCCCGA TGCCCACCAG GACTACTCCA
    GATCCCAAGC TAACCAGAGA AGTGGCTGAG AGTCCCCGGC CCCGGCGCTG GGCAGCCCAC
    GGGGCATCAC CAGAGGACTT CTCACTGACC CTGGGGGCAC GGGGCCGTAG GACTCGGAGT
    CCCTCACCCA CACTTGGGGA GTCCCTGGCG CCCCGCAAGG GCAGCTTCAG TGGCAGGCTG
    AGCCCGGCCT ACAGTCTGGG CTCTCTGACA GGGGCTTCGC CCCGTCAGAG CCCCCGCGCC
    CAGAGGAAGC TGTCCAGTGG GGACCTGCGG GTACCTGTCA CTCGGGAGCG GAAAAATAGC
    ATTACGGAGA TCAGCGACAA TGAGGACGAC CTCCTGGAGT ACCACCGGCG GCAGCGCCAA
    GAACGGCTCT GGGAGCAGGA GATGGAGAGG CTGGAGCGCC AGCGCCTGGA GACCATACTG
    AACCTGTGTG CCGAGTACAG CCGTGCTGAT GGGGGCCTCG AGGCGGGGGA GCTGCCTAGC
    ATCGGGGAGG CCGCCGCGGC ACTGGCGCTG GCGGGCCGCA GGCCCTCACG AGGCCTTTCT
    GGGGCCCCCG GAGCCTCGGG GCGGAGCACT GAAGAGCCTG GGGGTGCCAC CCCGCGCCTG
    TGGGAGTGTG TGGAGCGCTC AGATGAGGAG AACCTGAAGG AGGAGTGCAG CAGCACGGAG
    AGCACCCAGC AGGAGCACGA AGATGCCCCT AGCACCAAGC TTCAGGGAGA GGTGCTGGCT
    CTGGAAGAGG AGCGGGCTCA GGTGCTGGGG CGTGTGGAGC AGCTCAAAGT CCGCGTGAAG
    GAGCTGGAGC AGCAGCTGCA GGAGTCAGCC CGAGAGGCTG AGATGGAACG GGCGTTGCTG
    CAGGGGGAGA GGGAGGCAGA GCGGGCACTG CTGCAGAAGG AGCAGAAGGC GGTGGACCAG
    CTGCAGGAGA AGTTAGTGAC CTTGGAGACT GGCATCCAGA AGGAGAGGGA CAAGGAGAGG
    GCGGAGCTGG CCGCGGGACG GAGGCACCTG GAGGCCCGCC AGGCGCTCTA CGCCGAGCTC
    CAGACGCAGC TCGATAACTG CCCCGAGTCA GTGCGGGAAC AGTTACAGGA GCAGCTGAGA
    AGGGTCAGTT TCACCAGCCC CCCTTCCCTG GCCCCCGAGG GCCACCCACC CAGAAGAGAG
    GAGAGCAGTG GGACGGGACC ACCCCGGGCC CTGCAGTACC TGCCGGACCC GGACACCAGG
    GAGGCCGAGG CCCTGGAGAC GGAGACAAAG CTGTTTGAGG ACTTGGAGTT CCAGCAGCTG
    GAGCGGGAGA GCCGCGTGGA GGAGGAGCGG GAGCTGGCAG GCCAGGGGCT GCTCCGGAGC
    CAGGCCGAGC TGCTCCGCAG CATCACCAAG AGGAAGGAGC GCCTGGCAGT CCTGGACAGT
    CAGGCGGGGC AGATCCGGGC CCAGGCCGTG CAGGAGTCCG AGCGCCTGGC GAGGGACAAG
    AACGCTTCCC TGCAGCTGCT GCAGAAGGAG AAGGAGAGAC TGACTATGTT GGAGGGAAGA
    TACCACTCAC TCACAGGAGG CAGGCCCTTC CCGAAGACCA CGTCCACCCT CAAAGAGGCT
    GAACTGCTCA TCTCCGAGTC CTCAGAGATG GGGCTGGGGT CGGCGACTCT GGTCCCTTTC
    CCAGGGTCTT CTCAGCCCGG GGCCTTCTCT GTCCCCCTAA CCCAACCTGC TTCCACTCAG
    CTCTGCCCCA AAGCACAAGA GGAGTATGTG AGCCTGGCAG AGGTTCTCCA GCTGTGCTTC
    CGCTTGGACC CCCATGCTTC CGCCACCTCC CCCAGCGTGT TCGCCCAGCC CCTGCCCGAC
    AGTGAGTACG TGACGCTTGA GCAGCTAAAG GTGATGTGGG GCACCTCACC CGTGCCCACA
    GCCCCCGCGC CGGGCCTGCC TTTCTGGGCC TCTGCCTCCC GGGACCTGGT TCCCACCACC
    TGCCTTCCTC CCGTGCTGCC CTCTTCCTCC TCCTCCGCTT CTCTCACGCC TTCACCCAAG
    ATGGAGGAGC TGCTACTCCC TGCTGTAGAC TTAGAGCAGT GGTACCAGGA GCTGATGGCC
    GGGCTGGGGA CCGGCCCCGC TGCAGCCTCC CCGCGCTCCT CCCCCCCGCC TCTGCCCGCC
    AAAGCTTCCC GGCAGCTGCA GGTTTACCGC TCCAAGATGG ATGGTGAGGC CACCAGCCCC
    CTGCCCCGGA CCCGCAGTGG CCCCCTCCCC TCCTCCTCTG GTTCTTCTTC CTCTTCTTCG
    CAGCTCAGCG TGGCTACTCT GGGCCGGAGC CCCTCCCCAA AGAGTGCCCT ACTTGCCCAG
    AATGGCACAG GCAGCCTTCC TCGCAACCTG GCGGCCACGC TGCAGGACAT TGAGACCAAG
    CGCCAGCTGG CCCTGCAGCA GAAGGGCGAG TCGCTTCCTG CAGAGCCCCC CCCAGCAGAC
    GACCCAGCAG GACAGCAGGT GATCGAGGAG CAGCGGCGGC GCCTGGCCGA GCTGAAGCAG
    AAGGCTGCGG CCGAGGCGCA GTGCCAGTGG GACGCCCTGC ACGGGGCGGC CCCCTTCCCC
    CCGGGCCCCT CGGGCTTCCC CCCGCTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCT
    GGCCGGGAGC GCGGGGAGGA GGGCGAGCAC GCCTACGACA CCCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACAGGC GGCAACTCAG CCTGCTCGCC CGACAACATG
    TCCAGTGCGA GCGGCCTGGA TGTGGGCAAA ATCGAGGAGA TGGAGAAGAT GCTGAAAGAG
    GCACACGCCG AGAAGAGCCG GCTCATGGAG TCCAGGGAGC GGGAGATGGA GCTCCGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGAGGCGA GAACAGGTGG AACGGAGGCT ACAGAGCGAG
    AGCGCCAGGA GGCAGCAGCT GGTGGAGAAG GAGGTCAAGA TGCGAGAGAA ACAGTTTTCC
    CAGGCACGAC CCCTGACCCG CTACCTGCCA ATCCGGAAGG AGGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCGGGCCA CGGTGTCGAT ACCTGCCTGC ACGTGGTGCT TAGCAGCAAG
    GTCTGCCGCG GCTACTTGGT CAAGATGGGC GGCAAGATTA AATCATGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTATG TGGACAAGCA CGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAAGTGT ACTACGATCA CCTGCGCAGT
    GCAGCCAAGA GGAGGTTTTT CAGCTTTTCT GTGGTGACTG AGAGCCCGAA CCCAGCCCTC
    ACCTTCTGTG TGAAGACCCA CGACCGGCTG TATTACATGG TGGCCCCGTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATCGTCACG GGAGCGGAGG GCTACACTCA GTTCATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273934.1
    CDS362..5284
    Translation

    Target ORF information:

    RefSeq Version XM_022418226.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418226.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGAAT CAGAAAGCCT GGTGAACGGG AACCACACCC CACAGCCTGC CACCCGGGGA
    CCCTCCGCCT GTGCCAGCCA CAGCTCCCTG GTGAGCTCTA TTGAGAAGGA CCTGCAGGAA
    ATCATGGACT CACTGGTGCT AGAGGAGCCT GGGGCGGCAG GCAAGAAGCC TGCTGCGACT
    TCCCCACTGT CGCCGATGGC TAACGGTGGG CGCTACTTGC TGTCCCCGCC GACCAGCCCT
    GGTGCCATGT CCGTGGGCTC CAGCTATGAG AACACCTCTC CAGCCTTCTC TCCACTCTCC
    TCACCAGCCA GCAGTGGAAG CTGTGCCAGC CACTCACCCA GCGGGCAGGA GCCAGCACCT
    TCCATGCCCC CGCTGGTCCC TGCCCGTTCC TCCAGCTACC ATCTGGCTCT GCAGCCCCCA
    CAGCCCCGAC CGAGTGGTGC CCGCCCCTCT GAGAGCCCCC GGTTGGGCAG GAAAGGGGGT
    CACGAGAGGC CTCCCAGTCC TGGCCTCCGA GGTCTGCTGA CAGACAGCCC TGCAGCCACG
    GTCTTGGCTG AGGCCCGCAG AGCCACTGAG AGCCCCCGGC TGGGTGGACA GCTGCCCGTG
    GTGGCCATCA GCCTGAGTGA GTACCCGGCT TCCGGTGCCC GAAGCCAACC CACCAGCATT
    CCTGGCAGCC CCAAGTTCCA GCCTCCAGTC CCCGCTCCCC GAAACAAGAT CGGCACCCTC
    CAGGACCGTC CTCCCAGCCC TTTCCGTGAG CTGCCCAGCA CCGAGCGGGT GCTGACAACC
    AGCCCCTCAC GCCAGCTGGT GGGCCGGACA TTTTCAGATG GGTCAGTCGC CCGCACTCTG
    CAGCCCCCCG AGAGCCCCCG CCTGGGCCGG CGGGGCCTGG ACAGTATGCG GGAATTGCCC
    CCATTGAGCC CGTCACTGTC CCGACGGGCG CTGTCCCCGA TGCCCACCAG GACTACTCCA
    GATCCCAAGC TAACCAGAGA AGTGGCTGAG AGTCCCCGGC CCCGGCGCTG GGCAGCCCAC
    GGGGCATCAC CAGAGGACTT CTCACTGACC CTGGGGGCAC GGGGCCGTAG GACTCGGAGT
    CCCTCACCCA CACTTGGGGA GTCCCTGGCG CCCCGCAAGG GCAGCTTCAG TGGCAGGCTG
    AGCCCGGCCT ACAGTCTGGG CTCTCTGACA GGGGCTTCGC CCCGTCAGAG CCCCCGCGCC
    CAGAGGAAGC TGTCCAGTGG GGACCTGCGG GTACCTGTCA CTCGGGAGCG GAAAAATAGC
    ATTACGGAGA TCAGCGACAA TGAGGACGAC CTCCTGGAGT ACCACCGGCG GCAGCGCCAA
    GAACGGCTCT GGGAGCAGGA GATGGAGAGG CTGGAGCGCC AGCGCCTGGA GACCATACTG
    AACCTGTGTG CCGAGTACAG CCGTGCTGAT GGGGGCCTCG AGGCGGGGGA GCTGCCTAGC
    ATCGGGGAGG CCGCCGCGGC ACTGGCGCTG GCGGGCCGCA GGCCCTCACG AGGCCTTTCT
    GGGGCCCCCG GAGCCTCGGG GCGGAGCACT GAAGAGCCTG GGGGTGCCAC CCCGCGCCTG
    TGGGAGTGTG TGGAGCGCTC AGATGAGGAG AACCTGAAGG AGGAGTGCAG CAGCACGGAG
    AGCACCCAGC AGGAGCACGA AGATGCCCCT AGCACCAAGC TTCAGGGAGA GGTGCTGGCT
    CTGGAAGAGG AGCGGGCTCA GGTGCTGGGG CGTGTGGAGC AGCTCAAAGT CCGCGTGAAG
    GAGCTGGAGC AGCAGCTGCA GGAGTCAGCC CGAGAGGCTG AGATGGAACG GGCGTTGCTG
    CAGGGGGAGA GGGAGGCAGA GCGGGCACTG CTGCAGAAGG AGCAGAAGGC GGTGGACCAG
    CTGCAGGAGA AGTTAGTGAC CTTGGAGACT GGCATCCAGA AGGAGAGGGA CAAGGAGAGG
    GCGGAGCTGG CCGCGGGACG GAGGCACCTG GAGGCCCGCC AGGCGCTCTA CGCCGAGCTC
    CAGACGCAGC TCGATAACTG CCCCGAGTCA GTGCGGGAAC AGTTACAGGA GCAGCTGAGA
    AGGGTCAGTT TCACCAGCCC CCCTTCCCTG GCCCCCGAGG GCCACCCACC CAGAAGAGAG
    GAGAGCAGTG GGACGGGACC ACCCCGGGCC CTGCAGTACC TGCCGGACCC GGACACCAGG
    GAGGCCGAGG CCCTGGAGAC GGAGACAAAG CTGTTTGAGG ACTTGGAGTT CCAGCAGCTG
    GAGCGGGAGA GCCGCGTGGA GGAGGAGCGG GAGCTGGCAG GCCAGGGGCT GCTCCGGAGC
    CAGGCCGAGC TGCTCCGCAG CATCACCAAG AGGAAGGAGC GCCTGGCAGT CCTGGACAGT
    CAGGCGGGGC AGATCCGGGC CCAGGCCGTG CAGGAGTCCG AGCGCCTGGC GAGGGACAAG
    AACGCTTCCC TGCAGCTGCT GCAGAAGGAG AAGGAGAGAC TGACTATGTT GGAGGGAAGA
    TACCACTCAC TCACAGGAGG CAGGCCCTTC CCGAAGACCA CGTCCACCCT CAAAGAGGCT
    GAACTGCTCA TCTCCGAGTC CTCAGAGATG GGGCTGGGGT CGGCGACTCT GGTCCCTTTC
    CCAGGGTCTT CTCAGCCCGG GGCCTTCTCT GTCCCCCTAA CCCAACCTGC TTCCACTCAG
    CTCTGCCCCA AAGCACAAGA GGAGTATGTG AGCCTGGCAG AGGTTCTCCA GCTGTGCTTC
    CGCTTGGACC CCCATGCTTC CGCCACCTCC CCCAGCGTGT TCGCCCAGCC CCTGCCCGAC
    AGTGAGTACG TGACGCTTGA GCAGCTAAAG GTGATGTGGG GCACCTCACC CGTGCCCACA
    GCCCCCGCGC CGGGCCTGCC TTTCTGGGCC TCTGCCTCCC GGGACCTGGT TCCCACCACC
    TGCCTTCCTC CCGTGCTGCC CTCTTCCTCC TCCTCCGCTT CTCTCACGCC TTCACCCAAG
    ATGGAGGAGC TGCTACTCCC TGCTGTAGAC TTAGAGCAGT GGTACCAGGA GCTGATGGCC
    GGGCTGGGGA CCGGCCCCGC TGCAGCCTCC CCGCGCTCCT CCCCCCCGCC TCTGCCCGCC
    AAAGCTTCCC GGCAGCTGCA GGTTTACCGC TCCAAGATGG ATGGTGAGGC CACCAGCCCC
    CTGCCCCGGA CCCGCAGTGG CCCCCTCCCC TCCTCCTCTG GTTCTTCTTC CTCTTCTTCG
    CAGCTCAGCG TGGCTACTCT GGGCCGGAGC CCCTCCCCAA AGAGTGCCCT ACTTGCCCAG
    AATGGCACAG GCAGCCTTCC TCGCAACCTG GCGGCCACGC TGCAGGACAT TGAGACCAAG
    CGCCAGCTGG CCCTGCAGCA GAAGGGCGAG TCGCTTCCTG CAGAGCCCCC CCCAGCAGAC
    GACCCAGCAG GACAGCAGGT GATCGAGGAG CAGCGGCGGC GCCTGGCCGA GCTGAAGCAG
    AAGGCTGCGG CCGAGGCGCA GTGCCAGTGG GACGCCCTGC ACGGGGCGGC CCCCTTCCCC
    CCGGGCCCCT CGGGCTTCCC CCCGCTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCT
    GGCCGGGAGC GCGGGGAGGA GGGCGAGCAC GCCTACGACA CCCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACAGGC GGCAACTCAG CCTGCTCGCC CGACAACATG
    TCCAGTGCGA GCGGCCTGGA TGTGGGCAAA ATCGAGGAGA TGGAGAAGAT GCTGAAAGAG
    GCACACGCCG AGAAGAGCCG GCTCATGGAG TCCAGGGAGC GGGAGATGGA GCTCCGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGAGGCGA GAACAGGTGG AACGGAGGCT ACAGAGCGAG
    AGCGCCAGGA GGCAGCAGCT GGTGGAGAAG GAGGTCAAGA TGCGAGAGAA ACAGTTTTCC
    CAGGCACGAC CCCTGACCCG CTACCTGCCA ATCCGGAAGG AGGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCGGGCCA CGGTGTCGAT ACCTGCCTGC ACGTGGTGCT TAGCAGCAAG
    GTCTGCCGCG GCTACTTGGT CAAGATGGGC GGCAAGATTA AATCATGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTATG TGGACAAGCA CGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAAGTGT ACTACGATCA CCTGCGCAGT
    GCAGCCAAGA GGAGGTTTTT CAGCTTTTCT GTGGTGACTG AGAGCCCGAA CCCAGCCCTC
    ACCTTCTGTG TGAAGACCCA CGACCGGCTG TATTACATGG TGGCCCCGTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATCGTCACG GGAGCGGAGG GCTACACTCA GTTCATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287935
    Clone ID Related Accession (Same CDS sequence) XM_022418227.1
    Accession Version XM_022418227.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4893bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC
    TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC
    GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC
    ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC
    AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA
    GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG
    CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC
    CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC
    ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA
    GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC
    GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT
    TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC
    AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC
    AGTGCAGCCA AGAGCCCGAA CCCAGCCCTC ACCTTCTGTG TGAAGACCCA CGACCGGCTG
    TATTACATGG TGGCCCCGTC TGCAGAGGCC ATGCGCATCT GGATGGACGT CATCGTCACG
    GGAGCGGAGG GCTACACTCA GTTCATGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273935.1
    CDS91..4983
    Translation

    Target ORF information:

    RefSeq Version XM_022418227.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418227.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC
    TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC
    GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC
    ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC
    AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA
    GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG
    CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC
    CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC
    ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA
    GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC
    GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT
    TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC
    AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC
    AGTGCAGCCA AGAGCCCGAA CCCAGCCCTC ACCTTCTGTG TGAAGACCCA CGACCGGCTG
    TATTACATGG TGGCCCCGTC TGCAGAGGCC ATGCGCATCT GGATGGACGT CATCGTCACG
    GGAGCGGAGG GCTACACTCA GTTCATGAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287936
    Clone ID Related Accession (Same CDS sequence) XM_022418228.1
    Accession Version XM_022418228.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC
    TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC
    GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC
    ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC
    AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC
    CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC
    GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC
    TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG
    GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG
    GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA
    CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG
    CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC
    GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA
    TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG
    GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC
    TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG
    AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG
    GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC
    TACACTCAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273936.1
    CDS360..5240
    Translation

    Target ORF information:

    RefSeq Version XM_022418228.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418228.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC
    TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC
    GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC
    ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC
    AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC
    CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC
    GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC
    TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG
    GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG
    GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA
    CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG
    CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC
    GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA
    TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG
    GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC
    TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG
    AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG
    GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC
    TACACTCAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287937
    Clone ID Related Accession (Same CDS sequence) XM_022418229.1
    Accession Version XM_022418229.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGATGCTC TTAATAGGAA CCAGGTAGGC CCTGGATGCA AGACCCAAGC AATGGTAAAG 
    AAAGGGCCCT TGGACCTGAT CGAGACAGGC AAAGGGCTGA AGGTGCAAAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG TGGGCGGCTG AGCACGGCTA TCACCCTTCT GCCTCTGGAG
    GAAGGGAAGA CGGTGATTGG CTCCGCAGCC AGGGACATCT CCCTGCAGGG CCCAGGCTTG
    GCTCCGGAGC ACTGCTACAT CGAGAACGTG CGGGGCACCC TCACCCTCTA CCCTTGTGGC
    AATGCCTGCA GTATTGACGG GCTCCCCATC CGGCAGCCCA CCAGGCTCAC TCAGGGCTGT
    ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA
    GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC
    TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC
    ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC
    CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT
    GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA
    CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC
    ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG
    CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC
    GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC
    TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG
    GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT
    GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG
    GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC
    CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG
    CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA
    TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT
    CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG
    GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC
    TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC
    CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG
    AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT
    ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA
    CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC
    CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC
    GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG
    GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG
    GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC
    ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG
    GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG
    CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG
    GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG
    CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG
    GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG
    ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG
    GTCAGTTTCA CCAGCCCCCC TTCCCTGGCC CCCGAGGGCC ACCCACCCAG AAGAGAGGAG
    AGCAGTGGGA CGGGACCACC CCGGGCCCTG CAGTACCTGC CGGACCCGGA CACCAGGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA
    CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA
    GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC
    TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC
    TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT
    GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC
    CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC
    CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG
    GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG
    CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA
    GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG
    CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG
    CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT
    GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC
    CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC
    CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG
    GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG
    GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC
    CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC
    AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC
    AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA
    CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG
    GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC
    GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG
    GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC
    ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC
    TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT
    GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG
    AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA
    GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC
    TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG
    CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273937.1
    CDS322..5181
    Translation

    Target ORF information:

    RefSeq Version XM_022418229.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418229.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGATGCTC TTAATAGGAA CCAGGTAGGC CCTGGATGCA AGACCCAAGC AATGGTAAAG 
    AAAGGGCCCT TGGACCTGAT CGAGACAGGC AAAGGGCTGA AGGTGCAAAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG TGGGCGGCTG AGCACGGCTA TCACCCTTCT GCCTCTGGAG
    GAAGGGAAGA CGGTGATTGG CTCCGCAGCC AGGGACATCT CCCTGCAGGG CCCAGGCTTG
    GCTCCGGAGC ACTGCTACAT CGAGAACGTG CGGGGCACCC TCACCCTCTA CCCTTGTGGC
    AATGCCTGCA GTATTGACGG GCTCCCCATC CGGCAGCCCA CCAGGCTCAC TCAGGGCTGT
    ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA
    GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC
    TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC
    ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC
    CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT
    GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA
    CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC
    ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG
    CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC
    GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC
    TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG
    GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT
    GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG
    GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC
    CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG
    CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA
    TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT
    CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG
    GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC
    TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC
    CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG
    AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT
    ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA
    CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC
    CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC
    GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG
    GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG
    GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC
    ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG
    GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG
    CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG
    GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG
    CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG
    GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG
    ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG
    GTCAGTTTCA CCAGCCCCCC TTCCCTGGCC CCCGAGGGCC ACCCACCCAG AAGAGAGGAG
    AGCAGTGGGA CGGGACCACC CCGGGCCCTG CAGTACCTGC CGGACCCGGA CACCAGGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA
    CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA
    GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC
    TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC
    TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT
    GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC
    CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC
    CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG
    GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG
    CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA
    GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG
    CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG
    CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT
    GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC
    CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC
    CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG
    GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG
    GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC
    CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC
    AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC
    AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA
    CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG
    GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC
    GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG
    GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC
    ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC
    TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT
    GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG
    AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA
    GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC
    TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG
    CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287938
    Clone ID Related Accession (Same CDS sequence) XM_022418230.1
    Accession Version XM_022418230.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGTACGTG ACGCTTGAGC AGCTAAAGGT GATGTGGGGC
    ACCTCACCCG TGCCCACAGC CCCCGCGCCG GGCCTGCCTT TCTGGGCCTC TGCCTCCCGG
    GACCTGGTTC CCACCACCTG CCTTCCTCCC GTGCTGCCCT CTTCCTCCTC CTCCGCTTCT
    CTCACGCCTT CACCCAAGAT GGAGGAGCTG CTACTCCCTG CTGTAGACTT AGAGCAGTGG
    TACCAGGAGC TGATGGCCGG GCTGGGGACC GGCCCCGCTG CAGCCTCCCC GCGCTCCTCC
    CCCCCGCCTC TGCCCGCCAA AGCTTCCCGG CAGCTGCAGG TTTACCGCTC CAAGATGGAT
    GGTGAGGCCA CCAGCCCCCT GCCCCGGACC CGCAGTGGCC CCCTCCCCTC CTCCTCTGGT
    TCTTCTTCCT CTTCTTCGCA GCTCAGCGTG GCTACTCTGG GCCGGAGCCC CTCCCCAAAG
    AGTGCCCTAC TTGCCCAGAA TGGCACAGGC AGCCTTCCTC GCAACCTGGC GGCCACGCTG
    CAGGACATTG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGGCGAGTC GCTTCCTGCA
    GAGCCCCCCC CAGCAGACGA CCCAGCAGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC
    CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC
    GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC
    TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG
    GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG
    GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA
    CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG
    CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC
    GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA
    TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG
    GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC
    TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG
    AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG
    GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC
    TACACTCAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273938.1
    CDS359..5179
    Translation

    Target ORF information:

    RefSeq Version XM_022418230.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418230.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGTACGTG ACGCTTGAGC AGCTAAAGGT GATGTGGGGC
    ACCTCACCCG TGCCCACAGC CCCCGCGCCG GGCCTGCCTT TCTGGGCCTC TGCCTCCCGG
    GACCTGGTTC CCACCACCTG CCTTCCTCCC GTGCTGCCCT CTTCCTCCTC CTCCGCTTCT
    CTCACGCCTT CACCCAAGAT GGAGGAGCTG CTACTCCCTG CTGTAGACTT AGAGCAGTGG
    TACCAGGAGC TGATGGCCGG GCTGGGGACC GGCCCCGCTG CAGCCTCCCC GCGCTCCTCC
    CCCCCGCCTC TGCCCGCCAA AGCTTCCCGG CAGCTGCAGG TTTACCGCTC CAAGATGGAT
    GGTGAGGCCA CCAGCCCCCT GCCCCGGACC CGCAGTGGCC CCCTCCCCTC CTCCTCTGGT
    TCTTCTTCCT CTTCTTCGCA GCTCAGCGTG GCTACTCTGG GCCGGAGCCC CTCCCCAAAG
    AGTGCCCTAC TTGCCCAGAA TGGCACAGGC AGCCTTCCTC GCAACCTGGC GGCCACGCTG
    CAGGACATTG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGGCGAGTC GCTTCCTGCA
    GAGCCCCCCC CAGCAGACGA CCCAGCAGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC
    CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC
    GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC
    TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG
    GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG
    GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA
    CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG
    CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC
    GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA
    TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG
    GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC
    TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG
    AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG
    GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC
    TACACTCAGT TCATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287939
    Clone ID Related Accession (Same CDS sequence) XM_022418231.1
    Accession Version XM_022418231.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGGAG TATGTGAGCC TGGCAGAGGT TCTCCAGCTG
    TGCTTCCGCT TGGACCCCCA TGCTTCCGCC ACCTCCCCCA GCGTGTTCGC CCAGCCCCTG
    CCCGACAGTG AGTACGTGAC GCTTGAGCAG CTAAAGGTGA TGTGGGGCAC CTCACCCGTG
    CCCACAGCCC CCGCGCCGGG CCTGCCTTTC TGGGCCTCTG CCTCCCGGGA CCTGGTTCCC
    ACCACCTGCC TTCCTCCCGT GCTGCCCTCT TCCTCCTCCT CCGCTTCTCT CACGCCTTCA
    CCCAAGATGG AGGAGCTGCT ACTCCCTGCT GTAGACTTAG AGCAGTGGTA CCAGGAGCTG
    ATGGCCGGGC TGGGGACCGG CCCCGCTGCA GCCTCCCCGC GCTCCTCCCC CCCGCCTCTG
    CCCGCCAAAG CTTCCCGGCA GCTGCAGGTT TACCGCTCCA AGATGGATGG TGAGGCCACC
    AGCCCCCTGC CCCGGACCCG CAGTGGCCCC CTCCCCTCCT CCTCTGGTTC TTCTTCCTCT
    TCTTCGCAGC TCAGCGTGGC TACTCTGGGC CGGAGCCCCT CCCCAAAGAG TGCCCTACTT
    GCCCAGAATG GCACAGGCAG CCTTCCTCGC AACCTGGCGG CCACGCTGCA GGACATTGAG
    ACCAAGCGCC AGCTGGCCCT GCAGCAGAAG GGCGAGTCGC TTCCTGCAGA GCCCCCCCCA
    GCAGACGACC CAGCAGGACA GCAGGTGATC GAGGAGCAGC GGCGGCGCCT GGCCGAGCTG
    AAGCAGAAGG CTGCGGCCGA GGCGCAGTGC CAGTGGGACG CCCTGCACGG GGCGGCCCCC
    TTCCCCCCGG GCCCCTCGGG CTTCCCCCCG CTCATGCACC ACTCCATCCT GCACCACCTG
    CCGGCTGGCC GGGAGCGCGG GGAGGAGGGC GAGCACGCCT ACGACACCCT GAGCCTGGAG
    AGCTCCGACA GCATGGAGAC CAGCATCTCC ACAGGCGGCA ACTCAGCCTG CTCGCCCGAC
    AACATGTCCA GTGCGAGCGG CCTGGATGTG GGCAAAATCG AGGAGATGGA GAAGATGCTG
    AAAGAGGCAC ACGCCGAGAA GAGCCGGCTC ATGGAGTCCA GGGAGCGGGA GATGGAGCTC
    CGGCGGCAGG CCCTGGAGGA GGAACGGAGG AGGCGAGAAC AGGTGGAACG GAGGCTACAG
    AGCGAGAGCG CCAGGAGGCA GCAGCTGGTG GAGAAGGAGG TCAAGATGCG AGAGAAACAG
    TTTTCCCAGG CACGACCCCT GACCCGCTAC CTGCCAATCC GGAAGGAGGA CTTTGACCTG
    AAGACCCACA TCGAGTCGTC GGGCCACGGT GTCGATACCT GCCTGCACGT GGTGCTTAGC
    AGCAAGGTCT GCCGCGGCTA CTTGGTCAAG ATGGGCGGCA AGATTAAATC ATGGAAGAAG
    CGCTGGTTTG TCTTCGACCG GCTCAAGCGC ACCCTTTCCT ATTATGTGGA CAAGCACGAG
    ACGAAGCTGA AGGGGGTCAT CTACTTCCAG GCCATCGAGG AAGTGTACTA CGATCACCTG
    CGCAGTGCAG CCAAGAGGAG GTTTTTCAGC TTTTCTGTGG TGACTGAGAG CCCGAACCCA
    GCCCTCACCT TCTGTGTGAA GACCCACGAC CGGCTGTATT ACATGGTGGC CCCGTCTGCA
    GAGGCCATGC GCATCTGGAT GGACGTCATC GTCACGGGAG CGGAGGGCTA CACTCAGTTC
    ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273939.1
    CDS91..4899
    Translation

    Target ORF information:

    RefSeq Version XM_022418231.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418231.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGGAG TATGTGAGCC TGGCAGAGGT TCTCCAGCTG
    TGCTTCCGCT TGGACCCCCA TGCTTCCGCC ACCTCCCCCA GCGTGTTCGC CCAGCCCCTG
    CCCGACAGTG AGTACGTGAC GCTTGAGCAG CTAAAGGTGA TGTGGGGCAC CTCACCCGTG
    CCCACAGCCC CCGCGCCGGG CCTGCCTTTC TGGGCCTCTG CCTCCCGGGA CCTGGTTCCC
    ACCACCTGCC TTCCTCCCGT GCTGCCCTCT TCCTCCTCCT CCGCTTCTCT CACGCCTTCA
    CCCAAGATGG AGGAGCTGCT ACTCCCTGCT GTAGACTTAG AGCAGTGGTA CCAGGAGCTG
    ATGGCCGGGC TGGGGACCGG CCCCGCTGCA GCCTCCCCGC GCTCCTCCCC CCCGCCTCTG
    CCCGCCAAAG CTTCCCGGCA GCTGCAGGTT TACCGCTCCA AGATGGATGG TGAGGCCACC
    AGCCCCCTGC CCCGGACCCG CAGTGGCCCC CTCCCCTCCT CCTCTGGTTC TTCTTCCTCT
    TCTTCGCAGC TCAGCGTGGC TACTCTGGGC CGGAGCCCCT CCCCAAAGAG TGCCCTACTT
    GCCCAGAATG GCACAGGCAG CCTTCCTCGC AACCTGGCGG CCACGCTGCA GGACATTGAG
    ACCAAGCGCC AGCTGGCCCT GCAGCAGAAG GGCGAGTCGC TTCCTGCAGA GCCCCCCCCA
    GCAGACGACC CAGCAGGACA GCAGGTGATC GAGGAGCAGC GGCGGCGCCT GGCCGAGCTG
    AAGCAGAAGG CTGCGGCCGA GGCGCAGTGC CAGTGGGACG CCCTGCACGG GGCGGCCCCC
    TTCCCCCCGG GCCCCTCGGG CTTCCCCCCG CTCATGCACC ACTCCATCCT GCACCACCTG
    CCGGCTGGCC GGGAGCGCGG GGAGGAGGGC GAGCACGCCT ACGACACCCT GAGCCTGGAG
    AGCTCCGACA GCATGGAGAC CAGCATCTCC ACAGGCGGCA ACTCAGCCTG CTCGCCCGAC
    AACATGTCCA GTGCGAGCGG CCTGGATGTG GGCAAAATCG AGGAGATGGA GAAGATGCTG
    AAAGAGGCAC ACGCCGAGAA GAGCCGGCTC ATGGAGTCCA GGGAGCGGGA GATGGAGCTC
    CGGCGGCAGG CCCTGGAGGA GGAACGGAGG AGGCGAGAAC AGGTGGAACG GAGGCTACAG
    AGCGAGAGCG CCAGGAGGCA GCAGCTGGTG GAGAAGGAGG TCAAGATGCG AGAGAAACAG
    TTTTCCCAGG CACGACCCCT GACCCGCTAC CTGCCAATCC GGAAGGAGGA CTTTGACCTG
    AAGACCCACA TCGAGTCGTC GGGCCACGGT GTCGATACCT GCCTGCACGT GGTGCTTAGC
    AGCAAGGTCT GCCGCGGCTA CTTGGTCAAG ATGGGCGGCA AGATTAAATC ATGGAAGAAG
    CGCTGGTTTG TCTTCGACCG GCTCAAGCGC ACCCTTTCCT ATTATGTGGA CAAGCACGAG
    ACGAAGCTGA AGGGGGTCAT CTACTTCCAG GCCATCGAGG AAGTGTACTA CGATCACCTG
    CGCAGTGCAG CCAAGAGGAG GTTTTTCAGC TTTTCTGTGG TGACTGAGAG CCCGAACCCA
    GCCCTCACCT TCTGTGTGAA GACCCACGAC CGGCTGTATT ACATGGTGGC CCCGTCTGCA
    GAGGCCATGC GCATCTGGAT GGACGTCATC GTCACGGGAG CGGAGGGCTA CACTCAGTTC
    ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287940
    Clone ID Related Accession (Same CDS sequence) XM_022418232.1
    Accession Version XM_022418232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA
    CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA
    GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC
    TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC
    TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT
    GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC
    CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC
    CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG
    GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG
    CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA
    GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG
    CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG
    CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT
    GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC
    CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC
    CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG
    GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG
    GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC
    CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC
    AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC
    AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA
    CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG
    GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC
    GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG
    GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC
    ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC
    TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT
    GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG
    AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA
    GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC
    TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG
    CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273940.1
    CDS90..4769
    Translation

    Target ORF information:

    RefSeq Version XM_022418232.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA
    CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA
    GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC
    TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC
    TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT
    GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC
    CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC
    CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG
    GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG
    CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA
    GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG
    CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG
    CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT
    GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC
    CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC
    CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG
    GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG
    GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC
    CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC
    AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC
    AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA
    CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG
    GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC
    GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG
    GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC
    ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC
    TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT
    GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG
    AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA
    GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC
    TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG
    CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287941
    Clone ID Related Accession (Same CDS sequence) XM_022418233.1
    Accession Version XM_022418233.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    TACGTGACGC TTGAGCAGCT AAAGGTGATG TGGGGCACCT CACCCGTGCC CACAGCCCCC
    GCGCCGGGCC TGCCTTTCTG GGCCTCTGCC TCCCGGGACC TGGTTCCCAC CACCTGCCTT
    CCTCCCGTGC TGCCCTCTTC CTCCTCCTCC GCTTCTCTCA CGCCTTCACC CAAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG CGAGTCGCTT CCTGCAGAGC CCCCCCCAGC AGACGACCCA
    GCAGGACAGC AGGTGATCGA GGAGCAGCGG CGGCGCCTGG CCGAGCTGAA GCAGAAGGCT
    GCGGCCGAGG CGCAGTGCCA GTGGGACGCC CTGCACGGGG CGGCCCCCTT CCCCCCGGGC
    CCCTCGGGCT TCCCCCCGCT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCTGGCCGG
    GAGCGCGGGG AGGAGGGCGA GCACGCCTAC GACACCCTGA GCCTGGAGAG CTCCGACAGC
    ATGGAGACCA GCATCTCCAC AGGCGGCAAC TCAGCCTGCT CGCCCGACAA CATGTCCAGT
    GCGAGCGGCC TGGATGTGGG CAAAATCGAG GAGATGGAGA AGATGCTGAA AGAGGCACAC
    GCCGAGAAGA GCCGGCTCAT GGAGTCCAGG GAGCGGGAGA TGGAGCTCCG GCGGCAGGCC
    CTGGAGGAGG AACGGAGGAG GCGAGAACAG GTGGAACGGA GGCTACAGAG CGAGAGCGCC
    AGGAGGCAGC AGCTGGTGGA GAAGGAGGTC AAGATGCGAG AGAAACAGTT TTCCCAGGCA
    CGACCCCTGA CCCGCTACCT GCCAATCCGG AAGGAGGACT TTGACCTGAA GACCCACATC
    GAGTCGTCGG GCCACGGTGT CGATACCTGC CTGCACGTGG TGCTTAGCAG CAAGGTCTGC
    CGCGGCTACT TGGTCAAGAT GGGCGGCAAG ATTAAATCAT GGAAGAAGCG CTGGTTTGTC
    TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TATGTGGACA AGCACGAGAC GAAGCTGAAG
    GGGGTCATCT ACTTCCAGGC CATCGAGGAA GTGTACTACG ATCACCTGCG CAGTGCAGCC
    AAGAGGAGGT TTTTCAGCTT TTCTGTGGTG ACTGAGAGCC CGAACCCAGC CCTCACCTTC
    TGTGTGAAGA CCCACGACCG GCTGTATTAC ATGGTGGCCC CGTCTGCAGA GGCCATGCGC
    ATCTGGATGG ACGTCATCGT CACGGGAGCG GAGGGCTACA CTCAGTTCAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273941.1
    CDS91..4767
    Translation

    Target ORF information:

    RefSeq Version XM_022418233.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418233.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    TACGTGACGC TTGAGCAGCT AAAGGTGATG TGGGGCACCT CACCCGTGCC CACAGCCCCC
    GCGCCGGGCC TGCCTTTCTG GGCCTCTGCC TCCCGGGACC TGGTTCCCAC CACCTGCCTT
    CCTCCCGTGC TGCCCTCTTC CTCCTCCTCC GCTTCTCTCA CGCCTTCACC CAAGATGGAG
    GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC
    CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC
    AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC
    ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG CGAGTCGCTT CCTGCAGAGC CCCCCCCAGC AGACGACCCA
    GCAGGACAGC AGGTGATCGA GGAGCAGCGG CGGCGCCTGG CCGAGCTGAA GCAGAAGGCT
    GCGGCCGAGG CGCAGTGCCA GTGGGACGCC CTGCACGGGG CGGCCCCCTT CCCCCCGGGC
    CCCTCGGGCT TCCCCCCGCT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCTGGCCGG
    GAGCGCGGGG AGGAGGGCGA GCACGCCTAC GACACCCTGA GCCTGGAGAG CTCCGACAGC
    ATGGAGACCA GCATCTCCAC AGGCGGCAAC TCAGCCTGCT CGCCCGACAA CATGTCCAGT
    GCGAGCGGCC TGGATGTGGG CAAAATCGAG GAGATGGAGA AGATGCTGAA AGAGGCACAC
    GCCGAGAAGA GCCGGCTCAT GGAGTCCAGG GAGCGGGAGA TGGAGCTCCG GCGGCAGGCC
    CTGGAGGAGG AACGGAGGAG GCGAGAACAG GTGGAACGGA GGCTACAGAG CGAGAGCGCC
    AGGAGGCAGC AGCTGGTGGA GAAGGAGGTC AAGATGCGAG AGAAACAGTT TTCCCAGGCA
    CGACCCCTGA CCCGCTACCT GCCAATCCGG AAGGAGGACT TTGACCTGAA GACCCACATC
    GAGTCGTCGG GCCACGGTGT CGATACCTGC CTGCACGTGG TGCTTAGCAG CAAGGTCTGC
    CGCGGCTACT TGGTCAAGAT GGGCGGCAAG ATTAAATCAT GGAAGAAGCG CTGGTTTGTC
    TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TATGTGGACA AGCACGAGAC GAAGCTGAAG
    GGGGTCATCT ACTTCCAGGC CATCGAGGAA GTGTACTACG ATCACCTGCG CAGTGCAGCC
    AAGAGGAGGT TTTTCAGCTT TTCTGTGGTG ACTGAGAGCC CGAACCCAGC CCTCACCTTC
    TGTGTGAAGA CCCACGACCG GCTGTATTAC ATGGTGGCCC CGTCTGCAGA GGCCATGCGC
    ATCTGGATGG ACGTCATCGT CACGGGAGCG GAGGGCTACA CTCAGTTCAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287942
    Clone ID Related Accession (Same CDS sequence) XM_022418234.1
    Accession Version XM_022418234.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    ATGGAGGAGC TGCTACTCCC TGCTGTAGAC TTAGAGCAGT GGTACCAGGA GCTGATGGCC
    GGGCTGGGGA CCGGCCCCGC TGCAGCCTCC CCGCGCTCCT CCCCCCCGCC TCTGCCCGCC
    AAAGCTTCCC GGCAGCTGCA GGTTTACCGC TCCAAGATGG ATGGTGAGGC CACCAGCCCC
    CTGCCCCGGA CCCGCAGTGG CCCCCTCCCC TCCTCCTCTG GTTCTTCTTC CTCTTCTTCG
    CAGCTCAGCG TGGCTACTCT GGGCCGGAGC CCCTCCCCAA AGAGTGCCCT ACTTGCCCAG
    AATGGCACAG GCAGCCTTCC TCGCAACCTG GCGGCCACGC TGCAGGACAT TGAGACCAAG
    CGCCAGCTGG CCCTGCAGCA GAAGGGCGAG TCGCTTCCTG CAGAGCCCCC CCCAGCAGAC
    GACCCAGCAG GACAGCAGGT GATCGAGGAG CAGCGGCGGC GCCTGGCCGA GCTGAAGCAG
    AAGGCTGCGG CCGAGGCGCA GTGCCAGTGG GACGCCCTGC ACGGGGCGGC CCCCTTCCCC
    CCGGGCCCCT CGGGCTTCCC CCCGCTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCT
    GGCCGGGAGC GCGGGGAGGA GGGCGAGCAC GCCTACGACA CCCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACAGGC GGCAACTCAG CCTGCTCGCC CGACAACATG
    TCCAGTGCGA GCGGCCTGGA TGTGGGCAAA ATCGAGGAGA TGGAGAAGAT GCTGAAAGAG
    GCACACGCCG AGAAGAGCCG GCTCATGGAG TCCAGGGAGC GGGAGATGGA GCTCCGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGAGGCGA GAACAGGTGG AACGGAGGCT ACAGAGCGAG
    AGCGCCAGGA GGCAGCAGCT GGTGGAGAAG GAGGTCAAGA TGCGAGAGAA ACAGTTTTCC
    CAGGCACGAC CCCTGACCCG CTACCTGCCA ATCCGGAAGG AGGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCGGGCCA CGGTGTCGAT ACCTGCCTGC ACGTGGTGCT TAGCAGCAAG
    GTCTGCCGCG GCTACTTGGT CAAGATGGGC GGCAAGATTA AATCATGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTATG TGGACAAGCA CGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAAGTGT ACTACGATCA CCTGCGCAGT
    GCAGCCAAGA GGAGGTTTTT CAGCTTTTCT GTGGTGACTG AGAGCCCGAA CCCAGCCCTC
    ACCTTCTGTG TGAAGACCCA CGACCGGCTG TATTACATGG TGGCCCCGTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATCGTCACG GGAGCGGAGG GCTACACTCA GTTCATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273942.1
    CDS90..4592
    Translation

    Target ORF information:

    RefSeq Version XM_022418234.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418234.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    ATGGAGGAGC TGCTACTCCC TGCTGTAGAC TTAGAGCAGT GGTACCAGGA GCTGATGGCC
    GGGCTGGGGA CCGGCCCCGC TGCAGCCTCC CCGCGCTCCT CCCCCCCGCC TCTGCCCGCC
    AAAGCTTCCC GGCAGCTGCA GGTTTACCGC TCCAAGATGG ATGGTGAGGC CACCAGCCCC
    CTGCCCCGGA CCCGCAGTGG CCCCCTCCCC TCCTCCTCTG GTTCTTCTTC CTCTTCTTCG
    CAGCTCAGCG TGGCTACTCT GGGCCGGAGC CCCTCCCCAA AGAGTGCCCT ACTTGCCCAG
    AATGGCACAG GCAGCCTTCC TCGCAACCTG GCGGCCACGC TGCAGGACAT TGAGACCAAG
    CGCCAGCTGG CCCTGCAGCA GAAGGGCGAG TCGCTTCCTG CAGAGCCCCC CCCAGCAGAC
    GACCCAGCAG GACAGCAGGT GATCGAGGAG CAGCGGCGGC GCCTGGCCGA GCTGAAGCAG
    AAGGCTGCGG CCGAGGCGCA GTGCCAGTGG GACGCCCTGC ACGGGGCGGC CCCCTTCCCC
    CCGGGCCCCT CGGGCTTCCC CCCGCTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCT
    GGCCGGGAGC GCGGGGAGGA GGGCGAGCAC GCCTACGACA CCCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACAGGC GGCAACTCAG CCTGCTCGCC CGACAACATG
    TCCAGTGCGA GCGGCCTGGA TGTGGGCAAA ATCGAGGAGA TGGAGAAGAT GCTGAAAGAG
    GCACACGCCG AGAAGAGCCG GCTCATGGAG TCCAGGGAGC GGGAGATGGA GCTCCGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGAGGCGA GAACAGGTGG AACGGAGGCT ACAGAGCGAG
    AGCGCCAGGA GGCAGCAGCT GGTGGAGAAG GAGGTCAAGA TGCGAGAGAA ACAGTTTTCC
    CAGGCACGAC CCCTGACCCG CTACCTGCCA ATCCGGAAGG AGGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCGGGCCA CGGTGTCGAT ACCTGCCTGC ACGTGGTGCT TAGCAGCAAG
    GTCTGCCGCG GCTACTTGGT CAAGATGGGC GGCAAGATTA AATCATGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTATG TGGACAAGCA CGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAAGTGT ACTACGATCA CCTGCGCAGT
    GCAGCCAAGA GGAGGTTTTT CAGCTTTTCT GTGGTGACTG AGAGCCCGAA CCCAGCCCTC
    ACCTTCTGTG TGAAGACCCA CGACCGGCTG TATTACATGG TGGCCCCGTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATCGTCACG GGAGCGGAGG GCTACACTCA GTTCATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287943
    Clone ID Related Accession (Same CDS sequence) XM_022418235.1
    Accession Version XM_022418235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG 
    ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA
    GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC
    TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC
    ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC
    CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT
    GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA
    CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC
    ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG
    CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC
    GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC
    TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG
    GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT
    GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG
    GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC
    CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG
    CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA
    TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT
    CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG
    GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC
    TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC
    CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG
    AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT
    ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA
    CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC
    CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC
    GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG
    GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG
    GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC
    ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG
    GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG
    CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG
    GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG
    CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG
    GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG
    ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG
    GTCAGTTTCA CCAGCCCCCC TTCCCTGGCC CCCGAGGGCC ACCCACCCAG AAGAGAGGAG
    AGCAGTGGGA CGGGACCACC CCGGGCCCTG CAGTACCTGC CGGACCCGGA CACCAGGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA
    CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA
    GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC
    TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC
    TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT
    GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC
    CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC
    CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG
    GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG
    CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA
    GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG
    CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG
    CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT
    GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC
    CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC
    CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG
    GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG
    GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC
    CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC
    AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC
    AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA
    CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG
    GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC
    GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG
    GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC
    ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC
    TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT
    GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG
    AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA
    GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC
    TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG
    CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273943.1
    CDS140..4639
    Translation

    Target ORF information:

    RefSeq Version XM_022418235.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG 
    ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA
    GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC
    TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC
    ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC
    CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT
    GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA
    CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC
    ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG
    CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC
    GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC
    TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG
    GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT
    GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG
    GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC
    CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG
    CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA
    TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT
    CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG
    GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC
    TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC
    CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG
    AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT
    ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA
    CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC
    CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC
    GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG
    GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG
    GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC
    ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG
    GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG
    CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG
    GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG
    CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG
    GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG
    ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG
    GTCAGTTTCA CCAGCCCCCC TTCCCTGGCC CCCGAGGGCC ACCCACCCAG AAGAGAGGAG
    AGCAGTGGGA CGGGACCACC CCGGGCCCTG CAGTACCTGC CGGACCCGGA CACCAGGGAG
    GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG
    GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG
    GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC
    GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC
    CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA
    CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA
    GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC
    TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC
    TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT
    GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC
    CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC
    CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG
    GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG
    CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA
    GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG
    CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG
    CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT
    GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC
    CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC
    CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG
    GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG
    GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC
    CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC
    AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC
    AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA
    CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG
    GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC
    GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG
    GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC
    ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC
    TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT
    GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG
    AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA
    GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC
    TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG
    CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287944
    Clone ID Related Accession (Same CDS sequence) XM_022418236.1
    Accession Version XM_022418236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product pleckstrin homology-like domain family B member 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC
    TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC
    GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC
    ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC
    AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA
    GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG
    CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC
    CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC
    ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA
    GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC
    GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT
    TCCCAGCAGG TGGTGTCGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273944.1
    CDS394..4857
    Translation

    Target ORF information:

    RefSeq Version XM_022418236.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA
    GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC
    AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG
    CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG
    AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC
    AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC
    AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA
    AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG
    GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT
    TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT
    CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC
    TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC
    GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC
    ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC
    AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC
    CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT
    TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC
    CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA
    GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG
    CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC
    CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC
    ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA
    GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC
    GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT
    TCCCAGCAGG TGGTGTCGCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36310
    Clone ID Related Accession (Same CDS sequence) XM_014113376.1
    Accession Version XM_014113376.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC
    CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC
    CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG
    GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG
    CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA
    GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG
    CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG
    CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT
    GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC
    CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC
    CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG
    GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG
    GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC
    CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC
    AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC
    AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA
    CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG
    GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC
    GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG
    GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC
    ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC
    TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT
    GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG
    AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA
    GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC
    TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG
    CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968851.1
    CDS417..4976
    Translation

    Target ORF information:

    RefSeq Version XM_014113376.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113376.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC
    CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC
    CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG
    GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG
    CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA
    GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG
    CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG
    CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT
    GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC
    CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC
    CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG
    GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG
    GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC
    CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC
    AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC
    AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA
    CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG
    GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC
    GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG
    GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC
    ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC
    TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT
    GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG
    AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA
    GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC
    TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG
    CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36311
    Clone ID Related Accession (Same CDS sequence) XM_014113377.1
    Accession Version XM_014113377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product pleckstrin homology-like domain family B member 1 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG
    GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC
    CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT
    TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG
    TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC
    TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG
    GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT
    GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA
    AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG
    CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT
    GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG
    CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG
    CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC
    ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC
    ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA
    GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG
    ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG
    CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG
    GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG
    ATGCGAGAGA AACAGTTTTC CCAGCAGGTG GTGTCGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968852.1
    CDS426..4667
    Translation

    Target ORF information:

    RefSeq Version XM_014113377.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
    AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG
    GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC
    AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT
    CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA
    GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT
    TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG
    GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC
    AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT
    GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG
    GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG
    GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG
    ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC
    CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC
    TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA
    CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC
    CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG
    GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC
    ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC
    GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC
    ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC
    CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA
    ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT
    CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG
    CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT
    CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC
    CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG
    AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG
    CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC
    GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT
    AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC
    CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA
    CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT
    AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT
    TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC
    CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG
    GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG
    GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG
    AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG
    CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC
    CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG
    AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG
    CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG
    AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG
    CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC
    CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG
    GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG
    GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG
    GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA
    GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC
    CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC
    ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG
    GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC
    CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT
    TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG
    TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC
    TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG
    GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT
    GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA
    AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG
    CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT
    GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG
    CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG
    CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC
    ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC
    ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA
    GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG
    ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG
    CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG
    GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG
    ATGCGAGAGA AACAGTTTTC CCAGCAGGTG GTGTCGCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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