PHLDB1 cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following PHLDB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHLDB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239028 NM_001206042.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222841 XM_015474658.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222842 XM_005215999.4
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222843 XM_005216002.4
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222844 XM_010812486.2
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222845 XM_005216003.4
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222846 XM_005216004.4
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222847 XM_005216005.4
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222848 XM_005216006.4
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222849 XM_010812487.3
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222850 XM_005216007.3
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa222851 XM_005216008.4
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa222852 XM_024975347.1
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa222836 XM_005215996.4
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222836 XM_010812482.3
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222837 XM_010812483.3
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222838 XM_010812484.3
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222839 XM_024975344.1
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa222840 XM_010812485.3
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa37883 NM_001206042.1
Latest version!
Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26349 XM_005215996.3
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa26349 XM_010812482.2
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa26352 XM_005215999.3
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26355 XM_005216002.3
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26356 XM_005216003.3
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26357 XM_005216004.3
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26358 XM_005216005.3
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26359 XM_005216006.3
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26360 XM_005216007.2
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa26361 XM_005216008.3
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa52569 XM_010812483.2
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa52570 XM_010812484.2
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa52571 XM_010812485.2
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa52572 XM_010812486.1
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa52573 XM_010812487.2
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa92656 XM_015474658.1
Latest version!
Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239028
    Clone ID Related Accession (Same CDS sequence) NM_001206042.2
    Accession Version NM_001206042.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000015.1. On May 30, 2018 this sequence version replaced NM_001206042.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571258.2763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC
    ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192971.1
    CDS378..4520
    Translation

    Target ORF information:

    RefSeq Version NM_001206042.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206042.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC
    ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222841
    Clone ID Related Accession (Same CDS sequence) XM_015474658.2
    Accession Version XM_015474658.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGTACGTG
    ACGCTTGAGC AGCTAAAGGT GATGTGGGGC ACCTCGCCAG TGCCCACAGG CCCCGCGCCG
    GGCCTGCCTC CCTGGGCCCC TGCCTCCCAG GACCTGGTTC CCACCACCTG CCTTCCTCCC
    GCGCTGCCCT CTTCCTCCTC CTTCGCTTCT GTCACGCCTT CGCCCCAGAT GGAGAAGCTG
    CTGCTCCCTG CTGTAGACTT AGAGCAGTGG TACCAGGAGC TGATGGCCGG GCTGGGGACG
    GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC
    CAGCTGCAGG TTTACCGCTC CAAAATGGAT GGCGAGGCCA CCAGCCCCCT GCCCCGCACC
    CGCAGTGGGC CACTCCCCTC CTCCTCTGGC TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG
    GCTACCTTGG GCCGCAGCCC CTCACCCAAG AGCACTCTGC TCGCCCAGAA CGGCACCAGC
    AGCCTTCCTC GCAACCTGGC AGCCACACTG CAGGACATCG AGACCAAGCG CCAGCTGGCC
    CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC GAGCCCCTCC CAACCGACGA CCCAGCAGGG
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT
    GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG
    GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA
    GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC
    GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG
    AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG
    CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC
    CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC
    TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG
    AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG
    AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330144.1
    CDS392..4882
    Translation

    Target ORF information:

    RefSeq Version XM_015474658.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474658.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGTACGTG
    ACGCTTGAGC AGCTAAAGGT GATGTGGGGC ACCTCGCCAG TGCCCACAGG CCCCGCGCCG
    GGCCTGCCTC CCTGGGCCCC TGCCTCCCAG GACCTGGTTC CCACCACCTG CCTTCCTCCC
    GCGCTGCCCT CTTCCTCCTC CTTCGCTTCT GTCACGCCTT CGCCCCAGAT GGAGAAGCTG
    CTGCTCCCTG CTGTAGACTT AGAGCAGTGG TACCAGGAGC TGATGGCCGG GCTGGGGACG
    GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC
    CAGCTGCAGG TTTACCGCTC CAAAATGGAT GGCGAGGCCA CCAGCCCCCT GCCCCGCACC
    CGCAGTGGGC CACTCCCCTC CTCCTCTGGC TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG
    GCTACCTTGG GCCGCAGCCC CTCACCCAAG AGCACTCTGC TCGCCCAGAA CGGCACCAGC
    AGCCTTCCTC GCAACCTGGC AGCCACACTG CAGGACATCG AGACCAAGCG CCAGCTGGCC
    CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC GAGCCCCTCC CAACCGACGA CCCAGCAGGG
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT
    GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG
    GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA
    GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC
    GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG
    AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG
    CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC
    CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC
    TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG
    AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG
    AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222842
    Clone ID Related Accession (Same CDS sequence) XM_005215999.4
    Accession Version XM_005215999.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215999.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGAT GGAGAAGCTG CTGCTCCCTG CTGTAGACTT AGAGCAGTGG
    TACCAGGAGC TGATGGCCGG GCTGGGGACG GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC
    CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC CAGCTGCAGG TTTACCGCTC CAAAATGGAT
    GGCGAGGCCA CCAGCCCCCT GCCCCGCACC CGCAGTGGGC CACTCCCCTC CTCCTCTGGC
    TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG GCTACCTTGG GCCGCAGCCC CTCACCCAAG
    AGCACTCTGC TCGCCCAGAA CGGCACCAGC AGCCTTCCTC GCAACCTGGC AGCCACACTG
    CAGGACATCG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC
    GAGCCCCTCC CAACCGACGA CCCAGCAGGG CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG
    CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT GAGGCACAGT GCCAGTGGGA TGCCCTGCAC
    GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG GGCTTCCCGC CACTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCCTC GCGGGAGCGA GGCGAGGAGG GTGAGCACGC CTACGACACG
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACGGGGGG CAACTCAGTC
    TGCTCCCCTG ACAACATGTC CAGTGCTAGC GGCCTGGACA TGGGGAAGAT TGAGGAAATG
    GAGAAGATGC TGAAAGAAGC TCACGCGGAG AAGAGCCGAC TGATGGAGTC GAGGGAACGT
    GAGATGGAGC TGAGGCGGCA GGCCCTGGAG GAGGAACGGA GGCGGCGGGA GCAGGTTGAA
    AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG CAGCAGCTTG TGGAGAAGGA GGTCAAGATG
    CGGGAGAAGC AGTTCTCACA GGCACGACCC CTGACCCGTT ACCTGCCAAT CCGGAAGGAA
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCAGGCCATG GTGTGGATAC CTGTCTGCAC
    GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC TACTTGGTCA AGATGGGCGG CAAGATCAAG
    TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTACGTG
    GACAAGCATG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAGGTGTAC
    TATGACCACC TGCGCAGTGC CGCCAAGAAG AGGTTTCTGA GCTTCACTAT GGTGACTGAG
    AGCCCGAACC CGGCCCTCAC CTTCTGCGTG AAGACCCACG ACCGGCTGTA CTACATGGTG
    GCCCCCTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TTGTCACGGG GGCAGAGGGC
    TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216056.1
    CDS392..4852
    Translation

    Target ORF information:

    RefSeq Version XM_005215999.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215999.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGAT GGAGAAGCTG CTGCTCCCTG CTGTAGACTT AGAGCAGTGG
    TACCAGGAGC TGATGGCCGG GCTGGGGACG GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC
    CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC CAGCTGCAGG TTTACCGCTC CAAAATGGAT
    GGCGAGGCCA CCAGCCCCCT GCCCCGCACC CGCAGTGGGC CACTCCCCTC CTCCTCTGGC
    TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG GCTACCTTGG GCCGCAGCCC CTCACCCAAG
    AGCACTCTGC TCGCCCAGAA CGGCACCAGC AGCCTTCCTC GCAACCTGGC AGCCACACTG
    CAGGACATCG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC
    GAGCCCCTCC CAACCGACGA CCCAGCAGGG CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG
    CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT GAGGCACAGT GCCAGTGGGA TGCCCTGCAC
    GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG GGCTTCCCGC CACTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCCTC GCGGGAGCGA GGCGAGGAGG GTGAGCACGC CTACGACACG
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACGGGGGG CAACTCAGTC
    TGCTCCCCTG ACAACATGTC CAGTGCTAGC GGCCTGGACA TGGGGAAGAT TGAGGAAATG
    GAGAAGATGC TGAAAGAAGC TCACGCGGAG AAGAGCCGAC TGATGGAGTC GAGGGAACGT
    GAGATGGAGC TGAGGCGGCA GGCCCTGGAG GAGGAACGGA GGCGGCGGGA GCAGGTTGAA
    AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG CAGCAGCTTG TGGAGAAGGA GGTCAAGATG
    CGGGAGAAGC AGTTCTCACA GGCACGACCC CTGACCCGTT ACCTGCCAAT CCGGAAGGAA
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCAGGCCATG GTGTGGATAC CTGTCTGCAC
    GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC TACTTGGTCA AGATGGGCGG CAAGATCAAG
    TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTACGTG
    GACAAGCATG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAGGTGTAC
    TATGACCACC TGCGCAGTGC CGCCAAGAAG AGGTTTCTGA GCTTCACTAT GGTGACTGAG
    AGCCCGAACC CGGCCCTCAC CTTCTGCGTG AAGACCCACG ACCGGCTGTA CTACATGGTG
    GCCCCCTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TTGTCACGGG GGCAGAGGGC
    TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222843
    Clone ID Related Accession (Same CDS sequence) XM_005216002.4
    Accession Version XM_005216002.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216002.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGCCAGC TGCAGGTTTA CCGCTCCAAA ATGGATGGCG AGGCCACCAG CCCCCTGCCC
    CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC
    AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC
    ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT CCTGCCGAGC CCCTCCCAAC CGACGACCCA
    GCAGGGCAGC AGGTGATCGA GGAGCAGCGG CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC
    GCGGCTGAGG CACAGTGCCA GTGGGATGCC CTGCACGGGG TGGCACCCTT TCCCACCGGG
    CCCGCGGGCT TCCCGCCACT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCCTCGCGG
    GAGCGAGGCG AGGAGGGTGA GCACGCCTAC GACACGCTGA GCCTGGAGAG CTCCGACAGC
    ATGGAGACCA GCATCTCCAC GGGGGGCAAC TCAGTCTGCT CCCCTGACAA CATGTCCAGT
    GCTAGCGGCC TGGACATGGG GAAGATTGAG GAAATGGAGA AGATGCTGAA AGAAGCTCAC
    GCGGAGAAGA GCCGACTGAT GGAGTCGAGG GAACGTGAGA TGGAGCTGAG GCGGCAGGCC
    CTGGAGGAGG AACGGAGGCG GCGGGAGCAG GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC
    CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC AAGATGCGGG AGAAGCAGTT CTCACAGGCA
    CGACCCCTGA CCCGTTACCT GCCAATCCGG AAGGAAGACT TTGACCTGAA GACCCACATC
    GAGTCGTCAG GCCATGGTGT GGATACCTGT CTGCACGTGG TGCTCAGCAG CAAGGTCTGC
    CGTGGCTACT TGGTCAAGAT GGGCGGCAAG ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC
    TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TACGTGGACA AGCATGAGAC GAAGCTGAAG
    GGGGTCATCT ACTTCCAGGC CATCGAGGAG GTGTACTATG ACCACCTGCG CAGTGCCGCC
    AAGAAGAGGT TTCTGAGCTT CACTATGGTG ACTGAGAGCC CGAACCCGGC CCTCACCTTC
    TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC
    ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216059.1
    CDS391..4707
    Translation

    Target ORF information:

    RefSeq Version XM_005216002.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216002.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGCCAGC TGCAGGTTTA CCGCTCCAAA ATGGATGGCG AGGCCACCAG CCCCCTGCCC
    CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC
    AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC
    ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT CCTGCCGAGC CCCTCCCAAC CGACGACCCA
    GCAGGGCAGC AGGTGATCGA GGAGCAGCGG CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC
    GCGGCTGAGG CACAGTGCCA GTGGGATGCC CTGCACGGGG TGGCACCCTT TCCCACCGGG
    CCCGCGGGCT TCCCGCCACT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCCTCGCGG
    GAGCGAGGCG AGGAGGGTGA GCACGCCTAC GACACGCTGA GCCTGGAGAG CTCCGACAGC
    ATGGAGACCA GCATCTCCAC GGGGGGCAAC TCAGTCTGCT CCCCTGACAA CATGTCCAGT
    GCTAGCGGCC TGGACATGGG GAAGATTGAG GAAATGGAGA AGATGCTGAA AGAAGCTCAC
    GCGGAGAAGA GCCGACTGAT GGAGTCGAGG GAACGTGAGA TGGAGCTGAG GCGGCAGGCC
    CTGGAGGAGG AACGGAGGCG GCGGGAGCAG GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC
    CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC AAGATGCGGG AGAAGCAGTT CTCACAGGCA
    CGACCCCTGA CCCGTTACCT GCCAATCCGG AAGGAAGACT TTGACCTGAA GACCCACATC
    GAGTCGTCAG GCCATGGTGT GGATACCTGT CTGCACGTGG TGCTCAGCAG CAAGGTCTGC
    CGTGGCTACT TGGTCAAGAT GGGCGGCAAG ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC
    TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TACGTGGACA AGCATGAGAC GAAGCTGAAG
    GGGGTCATCT ACTTCCAGGC CATCGAGGAG GTGTACTATG ACCACCTGCG CAGTGCCGCC
    AAGAAGAGGT TTCTGAGCTT CACTATGGTG ACTGAGAGCC CGAACCCGGC CCTCACCTTC
    TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC
    ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222844
    Clone ID Related Accession (Same CDS sequence) XM_010812486.2
    Accession Version XM_010812486.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810788.1
    CDS149..4423
    Translation

    Target ORF information:

    RefSeq Version XM_010812486.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812486.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222845
    Clone ID Related Accession (Same CDS sequence) XM_005216003.4
    Accession Version XM_005216003.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216003.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGCCAGC TGCAGGTTTA CCGCTCCAAA ATGGATGGCG AGGCCACCAG CCCCCTGCCC
    CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC
    AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC
    ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG
    CTGAAGCAGA AGGCCGCGGC TGAGGCACAG TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA
    CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG CCACTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG GGTGAGCACG CCTACGACAC GCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACGGGGG GCAACTCAGT CTGCTCCCCT
    GACAACATGT CCAGTGCTAG CGGCCTGGAC ATGGGGAAGA TTGAGGAAAT GGAGAAGATG
    CTGAAAGAAG CTCACGCGGA GAAGAGCCGA CTGATGGAGT CGAGGGAACG TGAGATGGAG
    CTGAGGCGGC AGGCCCTGGA GGAGGAACGG AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG
    CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG
    CAGTTCTCAC AGGCACGACC CCTGACCCGT TACCTGCCAA TCCGGAAGGA AGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCAGGCCAT GGTGTGGATA CCTGTCTGCA CGTGGTGCTC
    AGCAGCAAGG TCTGCCGTGG CTACTTGGTC AAGATGGGCG GCAAGATCAA GTCGTGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTACGT GGACAAGCAT
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAGGTGTA CTATGACCAC
    CTGCGCAGTG CCGCCAAGAA GAGGTTTCTG AGCTTCACTA TGGTGACTGA GAGCCCGAAC
    CCGGCCCTCA CCTTCTGCGT GAAGACCCAC GACCGGCTGT ACTACATGGT GGCCCCCTCT
    GCAGAGGCCA TGCGCATCTG GATGGACGTC ATTGTCACGG GGGCAGAGGG CTACACTCAG
    TTTATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216060.1
    CDS391..4662
    Translation

    Target ORF information:

    RefSeq Version XM_005216003.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216003.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGCCAGC TGCAGGTTTA CCGCTCCAAA ATGGATGGCG AGGCCACCAG CCCCCTGCCC
    CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC
    AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC
    ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG
    CTGAAGCAGA AGGCCGCGGC TGAGGCACAG TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA
    CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG CCACTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG GGTGAGCACG CCTACGACAC GCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACGGGGG GCAACTCAGT CTGCTCCCCT
    GACAACATGT CCAGTGCTAG CGGCCTGGAC ATGGGGAAGA TTGAGGAAAT GGAGAAGATG
    CTGAAAGAAG CTCACGCGGA GAAGAGCCGA CTGATGGAGT CGAGGGAACG TGAGATGGAG
    CTGAGGCGGC AGGCCCTGGA GGAGGAACGG AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG
    CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG
    CAGTTCTCAC AGGCACGACC CCTGACCCGT TACCTGCCAA TCCGGAAGGA AGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCAGGCCAT GGTGTGGATA CCTGTCTGCA CGTGGTGCTC
    AGCAGCAAGG TCTGCCGTGG CTACTTGGTC AAGATGGGCG GCAAGATCAA GTCGTGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTACGT GGACAAGCAT
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAGGTGTA CTATGACCAC
    CTGCGCAGTG CCGCCAAGAA GAGGTTTCTG AGCTTCACTA TGGTGACTGA GAGCCCGAAC
    CCGGCCCTCA CCTTCTGCGT GAAGACCCAC GACCGGCTGT ACTACATGGT GGCCCCCTCT
    GCAGAGGCCA TGCGCATCTG GATGGACGTC ATTGTCACGG GGGCAGAGGG CTACACTCAG
    TTTATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222846
    Clone ID Related Accession (Same CDS sequence) XM_005216004.4
    Accession Version XM_005216004.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216004.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG TCGAGTCGCT TCCTGCCGAG CCCCTCCCAA CCGACGACCC AGCAGGGCAG
    CAGGTGATCG AGGAGCAGCG GCGGCGGCTG GCGGAGCTGA AGCAGAAGGC CGCGGCTGAG
    GCACAGTGCC AGTGGGATGC CCTGCACGGG GTGGCACCCT TTCCCACCGG GCCCGCGGGC
    TTCCCGCCAC TCATGCACCA CTCCATCCTG CACCACCTGC CGGCCTCGCG GGAGCGAGGC
    GAGGAGGGTG AGCACGCCTA CGACACGCTG AGCCTGGAGA GCTCCGACAG CATGGAGACC
    AGCATCTCCA CGGGGGGCAA CTCAGTCTGC TCCCCTGACA ACATGTCCAG TGCTAGCGGC
    CTGGACATGG GGAAGATTGA GGAAATGGAG AAGATGCTGA AAGAAGCTCA CGCGGAGAAG
    AGCCGACTGA TGGAGTCGAG GGAACGTGAG ATGGAGCTGA GGCGGCAGGC CCTGGAGGAG
    GAACGGAGGC GGCGGGAGCA GGTTGAAAGG AGGTTGCAGA GCGAGAGCGC CCGGAGGCAG
    CAGCTTGTGG AGAAGGAGGT CAAGATGCGG GAGAAGCAGT TCTCACAGGC ACGACCCCTG
    ACCCGTTACC TGCCAATCCG GAAGGAAGAC TTTGACCTGA AGACCCACAT CGAGTCGTCA
    GGCCATGGTG TGGATACCTG TCTGCACGTG GTGCTCAGCA GCAAGGTCTG CCGTGGCTAC
    TTGGTCAAGA TGGGCGGCAA GATCAAGTCG TGGAAGAAGC GCTGGTTTGT CTTCGACCGG
    CTCAAGCGCA CCCTTTCCTA TTACGTGGAC AAGCATGAGA CGAAGCTGAA GGGGGTCATC
    TACTTCCAGG CCATCGAGGA GGTGTACTAT GACCACCTGC GCAGTGCCGC CAAGAAGAGG
    TTTCTGAGCT TCACTATGGT GACTGAGAGC CCGAACCCGG CCCTCACCTT CTGCGTGAAG
    ACCCACGACC GGCTGTACTA CATGGTGGCC CCCTCTGCAG AGGCCATGCG CATCTGGATG
    GACGTCATTG TCACGGGGGC AGAGGGCTAC ACTCAGTTTA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216061.1
    CDS391..4578
    Translation

    Target ORF information:

    RefSeq Version XM_005216004.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216004.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG TCGAGTCGCT TCCTGCCGAG CCCCTCCCAA CCGACGACCC AGCAGGGCAG
    CAGGTGATCG AGGAGCAGCG GCGGCGGCTG GCGGAGCTGA AGCAGAAGGC CGCGGCTGAG
    GCACAGTGCC AGTGGGATGC CCTGCACGGG GTGGCACCCT TTCCCACCGG GCCCGCGGGC
    TTCCCGCCAC TCATGCACCA CTCCATCCTG CACCACCTGC CGGCCTCGCG GGAGCGAGGC
    GAGGAGGGTG AGCACGCCTA CGACACGCTG AGCCTGGAGA GCTCCGACAG CATGGAGACC
    AGCATCTCCA CGGGGGGCAA CTCAGTCTGC TCCCCTGACA ACATGTCCAG TGCTAGCGGC
    CTGGACATGG GGAAGATTGA GGAAATGGAG AAGATGCTGA AAGAAGCTCA CGCGGAGAAG
    AGCCGACTGA TGGAGTCGAG GGAACGTGAG ATGGAGCTGA GGCGGCAGGC CCTGGAGGAG
    GAACGGAGGC GGCGGGAGCA GGTTGAAAGG AGGTTGCAGA GCGAGAGCGC CCGGAGGCAG
    CAGCTTGTGG AGAAGGAGGT CAAGATGCGG GAGAAGCAGT TCTCACAGGC ACGACCCCTG
    ACCCGTTACC TGCCAATCCG GAAGGAAGAC TTTGACCTGA AGACCCACAT CGAGTCGTCA
    GGCCATGGTG TGGATACCTG TCTGCACGTG GTGCTCAGCA GCAAGGTCTG CCGTGGCTAC
    TTGGTCAAGA TGGGCGGCAA GATCAAGTCG TGGAAGAAGC GCTGGTTTGT CTTCGACCGG
    CTCAAGCGCA CCCTTTCCTA TTACGTGGAC AAGCATGAGA CGAAGCTGAA GGGGGTCATC
    TACTTCCAGG CCATCGAGGA GGTGTACTAT GACCACCTGC GCAGTGCCGC CAAGAAGAGG
    TTTCTGAGCT TCACTATGGT GACTGAGAGC CCGAACCCGG CCCTCACCTT CTGCGTGAAG
    ACCCACGACC GGCTGTACTA CATGGTGGCC CCCTCTGCAG AGGCCATGCG CATCTGGATG
    GACGTCATTG TCACGGGGGC AGAGGGCTAC ACTCAGTTTA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222847
    Clone ID Related Accession (Same CDS sequence) XM_005216005.4
    Accession Version XM_005216005.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216005.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAAA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC
    CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC
    AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC
    CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC
    GAGTCGCTTC CTGCCGAGCC CCTCCCAACC GACGACCCAG CAGGGCAGCA GGTGATCGAG
    GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG
    TGGGATGCCC TGCACGGGGT GGCACCCTTT CCCACCGGGC CCGCGGGCTT CCCGCCACTC
    ATGCACCACT CCATCCTGCA CCACCTGCCG GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG
    CACGCCTACG ACACGCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACG
    GGGGGCAACT CAGTCTGCTC CCCTGACAAC ATGTCCAGTG CTAGCGGCCT GGACATGGGG
    AAGATTGAGG AAATGGAGAA GATGCTGAAA GAAGCTCACG CGGAGAAGAG CCGACTGATG
    GAGTCGAGGG AACGTGAGAT GGAGCTGAGG CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG
    CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG
    AAGGAGGTCA AGATGCGGGA GAAGCAGTTC TCACAGGCAC GACCCCTGAC CCGTTACCTG
    CCAATCCGGA AGGAAGACTT TGACCTGAAG ACCCACATCG AGTCGTCAGG CCATGGTGTG
    GATACCTGTC TGCACGTGGT GCTCAGCAGC AAGGTCTGCC GTGGCTACTT GGTCAAGATG
    GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC
    CTTTCCTATT ACGTGGACAA GCATGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC
    ATCGAGGAGG TGTACTATGA CCACCTGCGC AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC
    ACTATGGTGA CTGAGAGCCC GAACCCGGCC CTCACCTTCT GCGTGAAGAC CCACGACCGG
    CTGTACTACA TGGTGGCCCC CTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATTGTC
    ACGGGGGCAG AGGGCTACAC TCAGTTTATG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216062.1
    CDS391..4566
    Translation

    Target ORF information:

    RefSeq Version XM_005216005.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216005.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAAA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC
    CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC
    AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC
    CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC
    GAGTCGCTTC CTGCCGAGCC CCTCCCAACC GACGACCCAG CAGGGCAGCA GGTGATCGAG
    GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG
    TGGGATGCCC TGCACGGGGT GGCACCCTTT CCCACCGGGC CCGCGGGCTT CCCGCCACTC
    ATGCACCACT CCATCCTGCA CCACCTGCCG GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG
    CACGCCTACG ACACGCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACG
    GGGGGCAACT CAGTCTGCTC CCCTGACAAC ATGTCCAGTG CTAGCGGCCT GGACATGGGG
    AAGATTGAGG AAATGGAGAA GATGCTGAAA GAAGCTCACG CGGAGAAGAG CCGACTGATG
    GAGTCGAGGG AACGTGAGAT GGAGCTGAGG CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG
    CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG
    AAGGAGGTCA AGATGCGGGA GAAGCAGTTC TCACAGGCAC GACCCCTGAC CCGTTACCTG
    CCAATCCGGA AGGAAGACTT TGACCTGAAG ACCCACATCG AGTCGTCAGG CCATGGTGTG
    GATACCTGTC TGCACGTGGT GCTCAGCAGC AAGGTCTGCC GTGGCTACTT GGTCAAGATG
    GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC
    CTTTCCTATT ACGTGGACAA GCATGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC
    ATCGAGGAGG TGTACTATGA CCACCTGCGC AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC
    ACTATGGTGA CTGAGAGCCC GAACCCGGCC CTCACCTTCT GCGTGAAGAC CCACGACCGG
    CTGTACTACA TGGTGGCCCC CTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATTGTC
    ACGGGGGCAG AGGGCTACAC TCAGTTTATG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222848
    Clone ID Related Accession (Same CDS sequence) XM_005216006.4
    Accession Version XM_005216006.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAAA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC
    CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC
    AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC
    CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGG
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT
    GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG
    GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA
    GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC
    GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG
    AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG
    CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC
    CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC
    TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG
    AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG
    AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216063.1
    CDS391..4521
    Translation

    Target ORF information:

    RefSeq Version XM_005216006.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216006.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAAA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC
    CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC
    AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC
    CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGG
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT
    GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG
    GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA
    GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC
    GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG
    AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG
    CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC
    CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC
    TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG
    AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG
    AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222849
    Clone ID Related Accession (Same CDS sequence) XM_010812487.3
    Accession Version XM_010812487.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC
    TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG
    GCAGAGGGCT ACACTCAGTT TATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810789.1
    CDS390..4499
    Translation

    Target ORF information:

    RefSeq Version XM_010812487.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812487.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC
    TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG
    GCAGAGGGCT ACACTCAGTT TATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222850
    Clone ID Related Accession (Same CDS sequence) XM_005216007.3
    Accession Version XM_005216007.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216007.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAA
    ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC
    TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA
    CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC
    ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT
    CCTGCCGAGC CCCTCCCAAC CGACGACCCA GCAGGGCAGC AGGTGATCGA GGAGCAGCGG
    CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC GCGGCTGAGG CACAGTGCCA GTGGGATGCC
    CTGCACGGGG TGGCACCCTT TCCCACCGGG CCCGCGGGCT TCCCGCCACT CATGCACCAC
    TCCATCCTGC ACCACCTGCC GGCCTCGCGG GAGCGAGGCG AGGAGGGTGA GCACGCCTAC
    GACACGCTGA GCCTGGAGAG CTCCGACAGC ATGGAGACCA GCATCTCCAC GGGGGGCAAC
    TCAGTCTGCT CCCCTGACAA CATGTCCAGT GCTAGCGGCC TGGACATGGG GAAGATTGAG
    GAAATGGAGA AGATGCTGAA AGAAGCTCAC GCGGAGAAGA GCCGACTGAT GGAGTCGAGG
    GAACGTGAGA TGGAGCTGAG GCGGCAGGCC CTGGAGGAGG AACGGAGGCG GCGGGAGCAG
    GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC
    AAGATGCGGG AGAAGCAGTT CTCACAGGCA CGACCCCTGA CCCGTTACCT GCCAATCCGG
    AAGGAAGACT TTGACCTGAA GACCCACATC GAGTCGTCAG GCCATGGTGT GGATACCTGT
    CTGCACGTGG TGCTCAGCAG CAAGGTCTGC CGTGGCTACT TGGTCAAGAT GGGCGGCAAG
    ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC TTCGACCGGC TCAAGCGCAC CCTTTCCTAT
    TACGTGGACA AGCATGAGAC GAAGCTGAAG GGGGTCATCT ACTTCCAGGC CATCGAGGAG
    GTGTACTATG ACCACCTGCG CAGTGCCGCC AAGAAGAGGT TTCTGAGCTT CACTATGGTG
    ACTGAGAGCC CGAACCCGGC CCTCACCTTC TGCGTGAAGA CCCACGACCG GCTGTACTAC
    ATGGTGGCCC CCTCTGCAGA GGCCATGCGC ATCTGGATGG ACGTCATTGT CACGGGGGCA
    GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216064.1
    CDS390..4436
    Translation

    Target ORF information:

    RefSeq Version XM_005216007.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216007.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAA
    ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC
    TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA
    CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC
    ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT
    CCTGCCGAGC CCCTCCCAAC CGACGACCCA GCAGGGCAGC AGGTGATCGA GGAGCAGCGG
    CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC GCGGCTGAGG CACAGTGCCA GTGGGATGCC
    CTGCACGGGG TGGCACCCTT TCCCACCGGG CCCGCGGGCT TCCCGCCACT CATGCACCAC
    TCCATCCTGC ACCACCTGCC GGCCTCGCGG GAGCGAGGCG AGGAGGGTGA GCACGCCTAC
    GACACGCTGA GCCTGGAGAG CTCCGACAGC ATGGAGACCA GCATCTCCAC GGGGGGCAAC
    TCAGTCTGCT CCCCTGACAA CATGTCCAGT GCTAGCGGCC TGGACATGGG GAAGATTGAG
    GAAATGGAGA AGATGCTGAA AGAAGCTCAC GCGGAGAAGA GCCGACTGAT GGAGTCGAGG
    GAACGTGAGA TGGAGCTGAG GCGGCAGGCC CTGGAGGAGG AACGGAGGCG GCGGGAGCAG
    GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC
    AAGATGCGGG AGAAGCAGTT CTCACAGGCA CGACCCCTGA CCCGTTACCT GCCAATCCGG
    AAGGAAGACT TTGACCTGAA GACCCACATC GAGTCGTCAG GCCATGGTGT GGATACCTGT
    CTGCACGTGG TGCTCAGCAG CAAGGTCTGC CGTGGCTACT TGGTCAAGAT GGGCGGCAAG
    ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC TTCGACCGGC TCAAGCGCAC CCTTTCCTAT
    TACGTGGACA AGCATGAGAC GAAGCTGAAG GGGGTCATCT ACTTCCAGGC CATCGAGGAG
    GTGTACTATG ACCACCTGCG CAGTGCCGCC AAGAAGAGGT TTCTGAGCTT CACTATGGTG
    ACTGAGAGCC CGAACCCGGC CCTCACCTTC TGCGTGAAGA CCCACGACCG GCTGTACTAC
    ATGGTGGCCC CCTCTGCAGA GGCCATGCGC ATCTGGATGG ACGTCATTGT CACGGGGGCA
    GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222851
    Clone ID Related Accession (Same CDS sequence) XM_005216008.4
    Accession Version XM_005216008.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216008.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAA
    ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC
    TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA
    CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC
    ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG
    ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG CTGAAGCAGA AGGCCGCGGC TGAGGCACAG
    TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG
    CCACTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG
    GGTGAGCACG CCTACGACAC GCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC
    TCCACGGGGG GCAACTCAGT CTGCTCCCCT GACAACATGT CCAGTGCTAG CGGCCTGGAC
    ATGGGGAAGA TTGAGGAAAT GGAGAAGATG CTGAAAGAAG CTCACGCGGA GAAGAGCCGA
    CTGATGGAGT CGAGGGAACG TGAGATGGAG CTGAGGCGGC AGGCCCTGGA GGAGGAACGG
    AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT
    GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG CAGTTCTCAC AGGCACGACC CCTGACCCGT
    TACCTGCCAA TCCGGAAGGA AGACTTTGAC CTGAAGACCC ACATCGAGTC GTCAGGCCAT
    GGTGTGGATA CCTGTCTGCA CGTGGTGCTC AGCAGCAAGG TCTGCCGTGG CTACTTGGTC
    AAGATGGGCG GCAAGATCAA GTCGTGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG
    CGCACCCTTT CCTATTACGT GGACAAGCAT GAGACGAAGC TGAAGGGGGT CATCTACTTC
    CAGGCCATCG AGGAGGTGTA CTATGACCAC CTGCGCAGTG CCGCCAAGAG CCCGAACCCG
    GCCCTCACCT TCTGCGTGAA GACCCACGAC CGGCTGTACT ACATGGTGGC CCCCTCTGCA
    GAGGCCATGC GCATCTGGAT GGACGTCATT GTCACGGGGG CAGAGGGCTA CACTCAGTTT
    ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216065.1
    CDS390..4358
    Translation

    Target ORF information:

    RefSeq Version XM_005216008.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216008.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAA
    ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC
    TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA
    CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC
    ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG
    ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG CTGAAGCAGA AGGCCGCGGC TGAGGCACAG
    TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG
    CCACTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG
    GGTGAGCACG CCTACGACAC GCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC
    TCCACGGGGG GCAACTCAGT CTGCTCCCCT GACAACATGT CCAGTGCTAG CGGCCTGGAC
    ATGGGGAAGA TTGAGGAAAT GGAGAAGATG CTGAAAGAAG CTCACGCGGA GAAGAGCCGA
    CTGATGGAGT CGAGGGAACG TGAGATGGAG CTGAGGCGGC AGGCCCTGGA GGAGGAACGG
    AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT
    GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG CAGTTCTCAC AGGCACGACC CCTGACCCGT
    TACCTGCCAA TCCGGAAGGA AGACTTTGAC CTGAAGACCC ACATCGAGTC GTCAGGCCAT
    GGTGTGGATA CCTGTCTGCA CGTGGTGCTC AGCAGCAAGG TCTGCCGTGG CTACTTGGTC
    AAGATGGGCG GCAAGATCAA GTCGTGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG
    CGCACCCTTT CCTATTACGT GGACAAGCAT GAGACGAAGC TGAAGGGGGT CATCTACTTC
    CAGGCCATCG AGGAGGTGTA CTATGACCAC CTGCGCAGTG CCGCCAAGAG CCCGAACCCG
    GCCCTCACCT TCTGCGTGAA GACCCACGAC CGGCTGTACT ACATGGTGGC CCCCTCTGCA
    GAGGCCATGC GCATCTGGAT GGACGTCATT GTCACGGGGG CAGAGGGCTA CACTCAGTTT
    ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222852
    Clone ID Related Accession (Same CDS sequence) XM_024975347.1
    Accession Version XM_024975347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC
    ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831115.1
    CDS148..3930
    Translation

    Target ORF information:

    RefSeq Version XM_024975347.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA AATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC
    ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222836
    Clone ID Related Accession (Same CDS sequence) XM_005215996.4 , XM_010812482.3
    Accession Version XM_005215996.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215996.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216053.1
    CDS392..5026
    Translation

    Target ORF information:

    RefSeq Version XM_005215996.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215996.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222836
    Clone ID Related Accession (Same CDS sequence) XM_005215996.4 , XM_010812482.3
    Accession Version XM_010812482.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812482.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810784.1
    CDS278..4912
    Translation

    Target ORF information:

    RefSeq Version XM_010812482.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812482.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222837
    Clone ID Related Accession (Same CDS sequence) XM_010812483.3
    Accession Version XM_010812483.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAGCCCGAAC CCGGCCCTCA CCTTCTGCGT GAAGACCCAC
    GACCGGCTGT ACTACATGGT GGCCCCCTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC
    ATTGTCACGG GGGCAGAGGG CTACACTCAG TTTATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810785.1
    CDS392..4993
    Translation

    Target ORF information:

    RefSeq Version XM_010812483.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812483.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAGCCCGAAC CCGGCCCTCA CCTTCTGCGT GAAGACCCAC
    GACCGGCTGT ACTACATGGT GGCCCCCTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC
    ATTGTCACGG GGGCAGAGGG CTACACTCAG TTTATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222838
    Clone ID Related Accession (Same CDS sequence) XM_010812484.3
    Accession Version XM_010812484.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG
    CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT
    GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC
    CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC
    TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC
    AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT
    GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG
    AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG
    CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG
    GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC
    CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC
    TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC
    AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC
    TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG
    GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAAGA GGTTTCTGAG CTTCACTATG
    GTGACTGAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC
    TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG
    GCAGAGGGCT ACACTCAGTT TATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810786.1
    CDS392..4981
    Translation

    Target ORF information:

    RefSeq Version XM_010812484.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812484.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG
    CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT
    GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC
    CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC
    TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC
    AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT
    GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG
    AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG
    CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG
    GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC
    CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC
    TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC
    AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC
    TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG
    GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAAGA GGTTTCTGAG CTTCACTATG
    GTGACTGAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC
    TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG
    GCAGAGGGCT ACACTCAGTT TATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222839
    Clone ID Related Accession (Same CDS sequence) XM_024975344.1
    Accession Version XM_024975344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG
    CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT
    GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC
    CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC
    TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC
    AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT
    GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG
    AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG
    CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG
    GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC
    CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC
    TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC
    AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC
    TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG
    GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAGCC CGAACCCGGC CCTCACCTTC
    TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC
    ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831112.1
    CDS392..4948
    Translation

    Target ORF information:

    RefSeq Version XM_024975344.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAAAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG
    CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT
    GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC
    CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC
    TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC
    AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT
    GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG
    AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG
    CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG
    GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC
    CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC
    TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC
    AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC
    TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG
    GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAGCC CGAACCCGGC CCTCACCTTC
    TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC
    ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222840
    Clone ID Related Accession (Same CDS sequence) XM_010812485.3
    Accession Version XM_010812485.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGCTGA TCTGCTCATC
    TCCGAGTCCT CAGAGGTGGG GCTGGGGACG GCAGCTCTGG GCCCCTTCCC AGAGTCTTCT
    CAGGCTGGGG CCTCCTCTCT TCCCTTAACC CCACCTGCTT CCACTCAGCT CTGCCTCAAA
    GCACAAGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC GCCAGTGCCC
    ACAGGCCCCG CGCCGGGCCT GCCTCCCTGG GCCCCTGCCT CCCAGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGCGCT GCCCTCTTCC TCCTCCTTCG CTTCTGTCAC GCCTTCGCCC
    CAGATGGAGA AGCTGCTGCT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACGGGCCC TGCTGCCGCC TCCCCTCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGCCAGCT GCAGGTTTAC CGCTCCAAAA TGGATGGCGA GGCCACCAGC
    CCCCTGCCCC GCACCCGCAG TGGGCCACTC CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC
    TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC
    CAGAACGGCA CCAGCAGCCT TCCTCGCAAC CTGGCAGCCA CACTGCAGGA CATCGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC GAGTCGCTTC CTGCCGAGCC CCTCCCAACC
    GACGACCCAG CAGGGCAGCA GGTGATCGAG GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG
    CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG TGGGATGCCC TGCACGGGGT GGCACCCTTT
    CCCACCGGGC CCGCGGGCTT CCCGCCACTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG CACGCCTACG ACACGCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACG GGGGGCAACT CAGTCTGCTC CCCTGACAAC
    ATGTCCAGTG CTAGCGGCCT GGACATGGGG AAGATTGAGG AAATGGAGAA GATGCTGAAA
    GAAGCTCACG CGGAGAAGAG CCGACTGATG GAGTCGAGGG AACGTGAGAT GGAGCTGAGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC
    GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG AAGGAGGTCA AGATGCGGGA GAAGCAGTTC
    TCACAGGCAC GACCCCTGAC CCGTTACCTG CCAATCCGGA AGGAAGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCAGG CCATGGTGTG GATACCTGTC TGCACGTGGT GCTCAGCAGC
    AAGGTCTGCC GTGGCTACTT GGTCAAGATG GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ACGTGGACAA GCATGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAGG TGTACTATGA CCACCTGCGC
    AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC ACTATGGTGA CTGAGAGCCC GAACCCGGCC
    CTCACCTTCT GCGTGAAGAC CCACGACCGG CTGTACTACA TGGTGGCCCC CTCTGCAGAG
    GCCATGCGCA TCTGGATGGA CGTCATTGTC ACGGGGGCAG AGGGCTACAC TCAGTTTATG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810787.1
    CDS392..4897
    Translation

    Target ORF information:

    RefSeq Version XM_010812485.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812485.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CAGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGCTGA TCTGCTCATC
    TCCGAGTCCT CAGAGGTGGG GCTGGGGACG GCAGCTCTGG GCCCCTTCCC AGAGTCTTCT
    CAGGCTGGGG CCTCCTCTCT TCCCTTAACC CCACCTGCTT CCACTCAGCT CTGCCTCAAA
    GCACAAGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC GCCAGTGCCC
    ACAGGCCCCG CGCCGGGCCT GCCTCCCTGG GCCCCTGCCT CCCAGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGCGCT GCCCTCTTCC TCCTCCTTCG CTTCTGTCAC GCCTTCGCCC
    CAGATGGAGA AGCTGCTGCT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACGGGCCC TGCTGCCGCC TCCCCTCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGCCAGCT GCAGGTTTAC CGCTCCAAAA TGGATGGCGA GGCCACCAGC
    CCCCTGCCCC GCACCCGCAG TGGGCCACTC CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC
    TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC
    CAGAACGGCA CCAGCAGCCT TCCTCGCAAC CTGGCAGCCA CACTGCAGGA CATCGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC GAGTCGCTTC CTGCCGAGCC CCTCCCAACC
    GACGACCCAG CAGGGCAGCA GGTGATCGAG GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG
    CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG TGGGATGCCC TGCACGGGGT GGCACCCTTT
    CCCACCGGGC CCGCGGGCTT CCCGCCACTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG CACGCCTACG ACACGCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACG GGGGGCAACT CAGTCTGCTC CCCTGACAAC
    ATGTCCAGTG CTAGCGGCCT GGACATGGGG AAGATTGAGG AAATGGAGAA GATGCTGAAA
    GAAGCTCACG CGGAGAAGAG CCGACTGATG GAGTCGAGGG AACGTGAGAT GGAGCTGAGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC
    GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG AAGGAGGTCA AGATGCGGGA GAAGCAGTTC
    TCACAGGCAC GACCCCTGAC CCGTTACCTG CCAATCCGGA AGGAAGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCAGG CCATGGTGTG GATACCTGTC TGCACGTGGT GCTCAGCAGC
    AAGGTCTGCC GTGGCTACTT GGTCAAGATG GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ACGTGGACAA GCATGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAGG TGTACTATGA CCACCTGCGC
    AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC ACTATGGTGA CTGAGAGCCC GAACCCGGCC
    CTCACCTTCT GCGTGAAGAC CCACGACCGG CTGTACTACA TGGTGGCCCC CTCTGCAGAG
    GCCATGCGCA TCTGGATGGA CGTCATTGTC ACGGGGGCAG AGGGCTACAC TCAGTTTATG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37883
    Clone ID Related Accession (Same CDS sequence) NM_001206042.1
    Accession Version NM_001206042.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02040474.1. On Apr 20, 2011 this sequence version replaced XM_613593.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822089, SAMN02822090 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC
    ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192971.1
    CDS378..4520
    Translation

    Target ORF information:

    RefSeq Version NM_001206042.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology like domain family B member 1 (PHLDB1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA AGAGGTTTCT GAGCTTCACT ATGGTGACTG AGAGCCCGAA CCCGGCCCTC
    ACCTTCTGCG TGAAGACCCA CGACCGGCTG TACTACATGG TGGCCCCCTC TGCAGAGGCC
    ATGCGCATCT GGATGGACGT CATTGTCACG GGGGCAGAGG GCTACACTCA GTTTATGAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26349
    Clone ID Related Accession (Same CDS sequence) XM_010812482.2 , XM_005215996.3
    Accession Version XM_010812482.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810784.1
    CDS281..4915
    Translation

    Target ORF information:

    RefSeq Version XM_010812482.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812482.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26349
    Clone ID Related Accession (Same CDS sequence) XM_010812482.2 , XM_005215996.3
    Accession Version XM_005215996.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215996.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216053.1
    CDS395..5029
    Translation

    Target ORF information:

    RefSeq Version XM_005215996.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215996.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26352
    Clone ID Related Accession (Same CDS sequence) XM_005215999.3
    Accession Version XM_005215999.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGAT GGAGAAGCTG CTGCTCCCTG CTGTAGACTT AGAGCAGTGG
    TACCAGGAGC TGATGGCCGG GCTGGGGACG GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC
    CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC CAGCTGCAGG TTTACCGCTC CAAGATGGAT
    GGCGAGGCCA CCAGCCCCCT GCCCCGCACC CGCAGTGGGC CACTCCCCTC CTCCTCTGGC
    TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG GCTACCTTGG GCCGCAGCCC CTCACCCAAG
    AGCACTCTGC TCGCCCAGAA CGGCACCAGC AGCCTTCCTC GCAACCTGGC AGCCACACTG
    CAGGACATCG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC
    GAGCCCCTCC CAACCGACGA CCCAGCAGGG CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG
    CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT GAGGCACAGT GCCAGTGGGA TGCCCTGCAC
    GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG GGCTTCCCGC CACTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCCTC GCGGGAGCGA GGCGAGGAGG GTGAGCACGC CTACGACACG
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACGGGGGG CAACTCAGTC
    TGCTCCCCTG ACAACATGTC CAGTGCTAGC GGCCTGGACA TGGGGAAGAT TGAGGAAATG
    GAGAAGATGC TGAAAGAAGC TCACGCGGAG AAGAGCCGAC TGATGGAGTC GAGGGAACGT
    GAGATGGAGC TGAGGCGGCA GGCCCTGGAG GAGGAACGGA GGCGGCGGGA GCAGGTTGAA
    AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG CAGCAGCTTG TGGAGAAGGA GGTCAAGATG
    CGGGAGAAGC AGTTCTCACA GGCACGACCC CTGACCCGTT ACCTGCCAAT CCGGAAGGAA
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCAGGCCATG GTGTGGATAC CTGTCTGCAC
    GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC TACTTGGTCA AGATGGGCGG CAAGATCAAG
    TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTACGTG
    GACAAGCATG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAGGTGTAC
    TATGACCACC TGCGCAGTGC CGCCAAGAAG AGGTTTCTGA GCTTCACTAT GGTGACTGAG
    AGCCCGAACC CGGCCCTCAC CTTCTGCGTG AAGACCCACG ACCGGCTGTA CTACATGGTG
    GCCCCCTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TTGTCACGGG GGCAGAGGGC
    TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216056.1
    CDS395..4855
    Translation

    Target ORF information:

    RefSeq Version XM_005215999.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215999.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGAT GGAGAAGCTG CTGCTCCCTG CTGTAGACTT AGAGCAGTGG
    TACCAGGAGC TGATGGCCGG GCTGGGGACG GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC
    CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC CAGCTGCAGG TTTACCGCTC CAAGATGGAT
    GGCGAGGCCA CCAGCCCCCT GCCCCGCACC CGCAGTGGGC CACTCCCCTC CTCCTCTGGC
    TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG GCTACCTTGG GCCGCAGCCC CTCACCCAAG
    AGCACTCTGC TCGCCCAGAA CGGCACCAGC AGCCTTCCTC GCAACCTGGC AGCCACACTG
    CAGGACATCG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC
    GAGCCCCTCC CAACCGACGA CCCAGCAGGG CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG
    CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT GAGGCACAGT GCCAGTGGGA TGCCCTGCAC
    GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG GGCTTCCCGC CACTCATGCA CCACTCCATC
    CTGCACCACC TGCCGGCCTC GCGGGAGCGA GGCGAGGAGG GTGAGCACGC CTACGACACG
    CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACGGGGGG CAACTCAGTC
    TGCTCCCCTG ACAACATGTC CAGTGCTAGC GGCCTGGACA TGGGGAAGAT TGAGGAAATG
    GAGAAGATGC TGAAAGAAGC TCACGCGGAG AAGAGCCGAC TGATGGAGTC GAGGGAACGT
    GAGATGGAGC TGAGGCGGCA GGCCCTGGAG GAGGAACGGA GGCGGCGGGA GCAGGTTGAA
    AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG CAGCAGCTTG TGGAGAAGGA GGTCAAGATG
    CGGGAGAAGC AGTTCTCACA GGCACGACCC CTGACCCGTT ACCTGCCAAT CCGGAAGGAA
    GACTTTGACC TGAAGACCCA CATCGAGTCG TCAGGCCATG GTGTGGATAC CTGTCTGCAC
    GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC TACTTGGTCA AGATGGGCGG CAAGATCAAG
    TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTACGTG
    GACAAGCATG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAGGTGTAC
    TATGACCACC TGCGCAGTGC CGCCAAGAAG AGGTTTCTGA GCTTCACTAT GGTGACTGAG
    AGCCCGAACC CGGCCCTCAC CTTCTGCGTG AAGACCCACG ACCGGCTGTA CTACATGGTG
    GCCCCCTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TTGTCACGGG GGCAGAGGGC
    TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26355
    Clone ID Related Accession (Same CDS sequence) XM_005216002.3
    Accession Version XM_005216002.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4317bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216002.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGCCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGCG AGGCCACCAG CCCCCTGCCC
    CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC
    AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC
    ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT CCTGCCGAGC CCCTCCCAAC CGACGACCCA
    GCAGGGCAGC AGGTGATCGA GGAGCAGCGG CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC
    GCGGCTGAGG CACAGTGCCA GTGGGATGCC CTGCACGGGG TGGCACCCTT TCCCACCGGG
    CCCGCGGGCT TCCCGCCACT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCCTCGCGG
    GAGCGAGGCG AGGAGGGTGA GCACGCCTAC GACACGCTGA GCCTGGAGAG CTCCGACAGC
    ATGGAGACCA GCATCTCCAC GGGGGGCAAC TCAGTCTGCT CCCCTGACAA CATGTCCAGT
    GCTAGCGGCC TGGACATGGG GAAGATTGAG GAAATGGAGA AGATGCTGAA AGAAGCTCAC
    GCGGAGAAGA GCCGACTGAT GGAGTCGAGG GAACGTGAGA TGGAGCTGAG GCGGCAGGCC
    CTGGAGGAGG AACGGAGGCG GCGGGAGCAG GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC
    CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC AAGATGCGGG AGAAGCAGTT CTCACAGGCA
    CGACCCCTGA CCCGTTACCT GCCAATCCGG AAGGAAGACT TTGACCTGAA GACCCACATC
    GAGTCGTCAG GCCATGGTGT GGATACCTGT CTGCACGTGG TGCTCAGCAG CAAGGTCTGC
    CGTGGCTACT TGGTCAAGAT GGGCGGCAAG ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC
    TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TACGTGGACA AGCATGAGAC GAAGCTGAAG
    GGGGTCATCT ACTTCCAGGC CATCGAGGAG GTGTACTATG ACCACCTGCG CAGTGCCGCC
    AAGAAGAGGT TTCTGAGCTT CACTATGGTG ACTGAGAGCC CGAACCCGGC CCTCACCTTC
    TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC
    ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216059.1
    CDS394..4710
    Translation

    Target ORF information:

    RefSeq Version XM_005216002.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216002.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGCCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGCG AGGCCACCAG CCCCCTGCCC
    CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC
    AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC
    ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT CCTGCCGAGC CCCTCCCAAC CGACGACCCA
    GCAGGGCAGC AGGTGATCGA GGAGCAGCGG CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC
    GCGGCTGAGG CACAGTGCCA GTGGGATGCC CTGCACGGGG TGGCACCCTT TCCCACCGGG
    CCCGCGGGCT TCCCGCCACT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCCTCGCGG
    GAGCGAGGCG AGGAGGGTGA GCACGCCTAC GACACGCTGA GCCTGGAGAG CTCCGACAGC
    ATGGAGACCA GCATCTCCAC GGGGGGCAAC TCAGTCTGCT CCCCTGACAA CATGTCCAGT
    GCTAGCGGCC TGGACATGGG GAAGATTGAG GAAATGGAGA AGATGCTGAA AGAAGCTCAC
    GCGGAGAAGA GCCGACTGAT GGAGTCGAGG GAACGTGAGA TGGAGCTGAG GCGGCAGGCC
    CTGGAGGAGG AACGGAGGCG GCGGGAGCAG GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC
    CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC AAGATGCGGG AGAAGCAGTT CTCACAGGCA
    CGACCCCTGA CCCGTTACCT GCCAATCCGG AAGGAAGACT TTGACCTGAA GACCCACATC
    GAGTCGTCAG GCCATGGTGT GGATACCTGT CTGCACGTGG TGCTCAGCAG CAAGGTCTGC
    CGTGGCTACT TGGTCAAGAT GGGCGGCAAG ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC
    TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TACGTGGACA AGCATGAGAC GAAGCTGAAG
    GGGGTCATCT ACTTCCAGGC CATCGAGGAG GTGTACTATG ACCACCTGCG CAGTGCCGCC
    AAGAAGAGGT TTCTGAGCTT CACTATGGTG ACTGAGAGCC CGAACCCGGC CCTCACCTTC
    TGCGTGAAGA CCCACGACCG GCTGTACTAC ATGGTGGCCC CCTCTGCAGA GGCCATGCGC
    ATCTGGATGG ACGTCATTGT CACGGGGGCA GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26356
    Clone ID Related Accession (Same CDS sequence) XM_005216003.3
    Accession Version XM_005216003.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGCCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGCG AGGCCACCAG CCCCCTGCCC
    CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC
    AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC
    ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG
    CTGAAGCAGA AGGCCGCGGC TGAGGCACAG TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA
    CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG CCACTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG GGTGAGCACG CCTACGACAC GCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACGGGGG GCAACTCAGT CTGCTCCCCT
    GACAACATGT CCAGTGCTAG CGGCCTGGAC ATGGGGAAGA TTGAGGAAAT GGAGAAGATG
    CTGAAAGAAG CTCACGCGGA GAAGAGCCGA CTGATGGAGT CGAGGGAACG TGAGATGGAG
    CTGAGGCGGC AGGCCCTGGA GGAGGAACGG AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG
    CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG
    CAGTTCTCAC AGGCACGACC CCTGACCCGT TACCTGCCAA TCCGGAAGGA AGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCAGGCCAT GGTGTGGATA CCTGTCTGCA CGTGGTGCTC
    AGCAGCAAGG TCTGCCGTGG CTACTTGGTC AAGATGGGCG GCAAGATCAA GTCGTGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTACGT GGACAAGCAT
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAGGTGTA CTATGACCAC
    CTGCGCAGTG CCGCCAAGAA GAGGTTTCTG AGCTTCACTA TGGTGACTGA GAGCCCGAAC
    CCGGCCCTCA CCTTCTGCGT GAAGACCCAC GACCGGCTGT ACTACATGGT GGCCCCCTCT
    GCAGAGGCCA TGCGCATCTG GATGGACGTC ATTGTCACGG GGGCAGAGGG CTACACTCAG
    TTTATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216060.1
    CDS394..4665
    Translation

    Target ORF information:

    RefSeq Version XM_005216003.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216003.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG
    AAGCTGCTGC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG
    GGGACGGGCC CTGCTGCCGC CTCCCCTCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT
    TCCCGCCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGCG AGGCCACCAG CCCCCTGCCC
    CGCACCCGCA GTGGGCCACT CCCCTCCTCC TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC
    AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA CCCAAGAGCA CTCTGCTCGC CCAGAACGGC
    ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC ACACTGCAGG ACATCGAGAC CAAGCGCCAG
    CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG
    CTGAAGCAGA AGGCCGCGGC TGAGGCACAG TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA
    CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG CCACTCATGC ACCACTCCAT CCTGCACCAC
    CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG GGTGAGCACG CCTACGACAC GCTGAGCCTG
    GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACGGGGG GCAACTCAGT CTGCTCCCCT
    GACAACATGT CCAGTGCTAG CGGCCTGGAC ATGGGGAAGA TTGAGGAAAT GGAGAAGATG
    CTGAAAGAAG CTCACGCGGA GAAGAGCCGA CTGATGGAGT CGAGGGAACG TGAGATGGAG
    CTGAGGCGGC AGGCCCTGGA GGAGGAACGG AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG
    CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG
    CAGTTCTCAC AGGCACGACC CCTGACCCGT TACCTGCCAA TCCGGAAGGA AGACTTTGAC
    CTGAAGACCC ACATCGAGTC GTCAGGCCAT GGTGTGGATA CCTGTCTGCA CGTGGTGCTC
    AGCAGCAAGG TCTGCCGTGG CTACTTGGTC AAGATGGGCG GCAAGATCAA GTCGTGGAAG
    AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTACGT GGACAAGCAT
    GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAGGTGTA CTATGACCAC
    CTGCGCAGTG CCGCCAAGAA GAGGTTTCTG AGCTTCACTA TGGTGACTGA GAGCCCGAAC
    CCGGCCCTCA CCTTCTGCGT GAAGACCCAC GACCGGCTGT ACTACATGGT GGCCCCCTCT
    GCAGAGGCCA TGCGCATCTG GATGGACGTC ATTGTCACGG GGGCAGAGGG CTACACTCAG
    TTTATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26357
    Clone ID Related Accession (Same CDS sequence) XM_005216004.3
    Accession Version XM_005216004.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG TCGAGTCGCT TCCTGCCGAG CCCCTCCCAA CCGACGACCC AGCAGGGCAG
    CAGGTGATCG AGGAGCAGCG GCGGCGGCTG GCGGAGCTGA AGCAGAAGGC CGCGGCTGAG
    GCACAGTGCC AGTGGGATGC CCTGCACGGG GTGGCACCCT TTCCCACCGG GCCCGCGGGC
    TTCCCGCCAC TCATGCACCA CTCCATCCTG CACCACCTGC CGGCCTCGCG GGAGCGAGGC
    GAGGAGGGTG AGCACGCCTA CGACACGCTG AGCCTGGAGA GCTCCGACAG CATGGAGACC
    AGCATCTCCA CGGGGGGCAA CTCAGTCTGC TCCCCTGACA ACATGTCCAG TGCTAGCGGC
    CTGGACATGG GGAAGATTGA GGAAATGGAG AAGATGCTGA AAGAAGCTCA CGCGGAGAAG
    AGCCGACTGA TGGAGTCGAG GGAACGTGAG ATGGAGCTGA GGCGGCAGGC CCTGGAGGAG
    GAACGGAGGC GGCGGGAGCA GGTTGAAAGG AGGTTGCAGA GCGAGAGCGC CCGGAGGCAG
    CAGCTTGTGG AGAAGGAGGT CAAGATGCGG GAGAAGCAGT TCTCACAGGC ACGACCCCTG
    ACCCGTTACC TGCCAATCCG GAAGGAAGAC TTTGACCTGA AGACCCACAT CGAGTCGTCA
    GGCCATGGTG TGGATACCTG TCTGCACGTG GTGCTCAGCA GCAAGGTCTG CCGTGGCTAC
    TTGGTCAAGA TGGGCGGCAA GATCAAGTCG TGGAAGAAGC GCTGGTTTGT CTTCGACCGG
    CTCAAGCGCA CCCTTTCCTA TTACGTGGAC AAGCATGAGA CGAAGCTGAA GGGGGTCATC
    TACTTCCAGG CCATCGAGGA GGTGTACTAT GACCACCTGC GCAGTGCCGC CAAGAAGAGG
    TTTCTGAGCT TCACTATGGT GACTGAGAGC CCGAACCCGG CCCTCACCTT CTGCGTGAAG
    ACCCACGACC GGCTGTACTA CATGGTGGCC CCCTCTGCAG AGGCCATGCG CATCTGGATG
    GACGTCATTG TCACGGGGGC AGAGGGCTAC ACTCAGTTTA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216061.1
    CDS393..4580
    Translation

    Target ORF information:

    RefSeq Version XM_005216004.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216004.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG TCGAGTCGCT TCCTGCCGAG CCCCTCCCAA CCGACGACCC AGCAGGGCAG
    CAGGTGATCG AGGAGCAGCG GCGGCGGCTG GCGGAGCTGA AGCAGAAGGC CGCGGCTGAG
    GCACAGTGCC AGTGGGATGC CCTGCACGGG GTGGCACCCT TTCCCACCGG GCCCGCGGGC
    TTCCCGCCAC TCATGCACCA CTCCATCCTG CACCACCTGC CGGCCTCGCG GGAGCGAGGC
    GAGGAGGGTG AGCACGCCTA CGACACGCTG AGCCTGGAGA GCTCCGACAG CATGGAGACC
    AGCATCTCCA CGGGGGGCAA CTCAGTCTGC TCCCCTGACA ACATGTCCAG TGCTAGCGGC
    CTGGACATGG GGAAGATTGA GGAAATGGAG AAGATGCTGA AAGAAGCTCA CGCGGAGAAG
    AGCCGACTGA TGGAGTCGAG GGAACGTGAG ATGGAGCTGA GGCGGCAGGC CCTGGAGGAG
    GAACGGAGGC GGCGGGAGCA GGTTGAAAGG AGGTTGCAGA GCGAGAGCGC CCGGAGGCAG
    CAGCTTGTGG AGAAGGAGGT CAAGATGCGG GAGAAGCAGT TCTCACAGGC ACGACCCCTG
    ACCCGTTACC TGCCAATCCG GAAGGAAGAC TTTGACCTGA AGACCCACAT CGAGTCGTCA
    GGCCATGGTG TGGATACCTG TCTGCACGTG GTGCTCAGCA GCAAGGTCTG CCGTGGCTAC
    TTGGTCAAGA TGGGCGGCAA GATCAAGTCG TGGAAGAAGC GCTGGTTTGT CTTCGACCGG
    CTCAAGCGCA CCCTTTCCTA TTACGTGGAC AAGCATGAGA CGAAGCTGAA GGGGGTCATC
    TACTTCCAGG CCATCGAGGA GGTGTACTAT GACCACCTGC GCAGTGCCGC CAAGAAGAGG
    TTTCTGAGCT TCACTATGGT GACTGAGAGC CCGAACCCGG CCCTCACCTT CTGCGTGAAG
    ACCCACGACC GGCTGTACTA CATGGTGGCC CCCTCTGCAG AGGCCATGCG CATCTGGATG
    GACGTCATTG TCACGGGGGC AGAGGGCTAC ACTCAGTTTA TGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26358
    Clone ID Related Accession (Same CDS sequence) XM_005216005.3
    Accession Version XM_005216005.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216005.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC
    CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC
    AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC
    CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC
    GAGTCGCTTC CTGCCGAGCC CCTCCCAACC GACGACCCAG CAGGGCAGCA GGTGATCGAG
    GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG
    TGGGATGCCC TGCACGGGGT GGCACCCTTT CCCACCGGGC CCGCGGGCTT CCCGCCACTC
    ATGCACCACT CCATCCTGCA CCACCTGCCG GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG
    CACGCCTACG ACACGCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACG
    GGGGGCAACT CAGTCTGCTC CCCTGACAAC ATGTCCAGTG CTAGCGGCCT GGACATGGGG
    AAGATTGAGG AAATGGAGAA GATGCTGAAA GAAGCTCACG CGGAGAAGAG CCGACTGATG
    GAGTCGAGGG AACGTGAGAT GGAGCTGAGG CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG
    CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG
    AAGGAGGTCA AGATGCGGGA GAAGCAGTTC TCACAGGCAC GACCCCTGAC CCGTTACCTG
    CCAATCCGGA AGGAAGACTT TGACCTGAAG ACCCACATCG AGTCGTCAGG CCATGGTGTG
    GATACCTGTC TGCACGTGGT GCTCAGCAGC AAGGTCTGCC GTGGCTACTT GGTCAAGATG
    GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC
    CTTTCCTATT ACGTGGACAA GCATGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC
    ATCGAGGAGG TGTACTATGA CCACCTGCGC AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC
    ACTATGGTGA CTGAGAGCCC GAACCCGGCC CTCACCTTCT GCGTGAAGAC CCACGACCGG
    CTGTACTACA TGGTGGCCCC CTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATTGTC
    ACGGGGGCAG AGGGCTACAC TCAGTTTATG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216062.1
    CDS393..4568
    Translation

    Target ORF information:

    RefSeq Version XM_005216005.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216005.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC
    CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC
    AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC
    CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC
    GAGTCGCTTC CTGCCGAGCC CCTCCCAACC GACGACCCAG CAGGGCAGCA GGTGATCGAG
    GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG
    TGGGATGCCC TGCACGGGGT GGCACCCTTT CCCACCGGGC CCGCGGGCTT CCCGCCACTC
    ATGCACCACT CCATCCTGCA CCACCTGCCG GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG
    CACGCCTACG ACACGCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACG
    GGGGGCAACT CAGTCTGCTC CCCTGACAAC ATGTCCAGTG CTAGCGGCCT GGACATGGGG
    AAGATTGAGG AAATGGAGAA GATGCTGAAA GAAGCTCACG CGGAGAAGAG CCGACTGATG
    GAGTCGAGGG AACGTGAGAT GGAGCTGAGG CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG
    CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG
    AAGGAGGTCA AGATGCGGGA GAAGCAGTTC TCACAGGCAC GACCCCTGAC CCGTTACCTG
    CCAATCCGGA AGGAAGACTT TGACCTGAAG ACCCACATCG AGTCGTCAGG CCATGGTGTG
    GATACCTGTC TGCACGTGGT GCTCAGCAGC AAGGTCTGCC GTGGCTACTT GGTCAAGATG
    GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC
    CTTTCCTATT ACGTGGACAA GCATGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC
    ATCGAGGAGG TGTACTATGA CCACCTGCGC AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC
    ACTATGGTGA CTGAGAGCCC GAACCCGGCC CTCACCTTCT GCGTGAAGAC CCACGACCGG
    CTGTACTACA TGGTGGCCCC CTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATTGTC
    ACGGGGGCAG AGGGCTACAC TCAGTTTATG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26359
    Clone ID Related Accession (Same CDS sequence) XM_005216006.3
    Accession Version XM_005216006.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC
    CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC
    AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC
    CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGG
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT
    GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG
    GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA
    GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC
    GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG
    AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG
    CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC
    CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC
    TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG
    AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG
    AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216063.1
    CDS393..4523
    Translation

    Target ORF information:

    RefSeq Version XM_005216006.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216006.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC
    CGCTCCAAGA TGGATGGCGA GGCCACCAGC CCCCTGCCCC GCACCCGCAG TGGGCCACTC
    CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC
    AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC CAGAACGGCA CCAGCAGCCT TCCTCGCAAC
    CTGGCAGCCA CACTGCAGGA CATCGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGG
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT
    GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG
    GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA
    GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC
    GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG
    AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG
    CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC
    CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC
    TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG
    AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG
    AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26360
    Clone ID Related Accession (Same CDS sequence) XM_005216007.2
    Accession Version XM_005216007.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAG
    ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC
    TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA
    CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC
    ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT
    CCTGCCGAGC CCCTCCCAAC CGACGACCCA GCAGGGCAGC AGGTGATCGA GGAGCAGCGG
    CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC GCGGCTGAGG CACAGTGCCA GTGGGATGCC
    CTGCACGGGG TGGCACCCTT TCCCACCGGG CCCGCGGGCT TCCCGCCACT CATGCACCAC
    TCCATCCTGC ACCACCTGCC GGCCTCGCGG GAGCGAGGCG AGGAGGGTGA GCACGCCTAC
    GACACGCTGA GCCTGGAGAG CTCCGACAGC ATGGAGACCA GCATCTCCAC GGGGGGCAAC
    TCAGTCTGCT CCCCTGACAA CATGTCCAGT GCTAGCGGCC TGGACATGGG GAAGATTGAG
    GAAATGGAGA AGATGCTGAA AGAAGCTCAC GCGGAGAAGA GCCGACTGAT GGAGTCGAGG
    GAACGTGAGA TGGAGCTGAG GCGGCAGGCC CTGGAGGAGG AACGGAGGCG GCGGGAGCAG
    GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC
    AAGATGCGGG AGAAGCAGTT CTCACAGGCA CGACCCCTGA CCCGTTACCT GCCAATCCGG
    AAGGAAGACT TTGACCTGAA GACCCACATC GAGTCGTCAG GCCATGGTGT GGATACCTGT
    CTGCACGTGG TGCTCAGCAG CAAGGTCTGC CGTGGCTACT TGGTCAAGAT GGGCGGCAAG
    ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC TTCGACCGGC TCAAGCGCAC CCTTTCCTAT
    TACGTGGACA AGCATGAGAC GAAGCTGAAG GGGGTCATCT ACTTCCAGGC CATCGAGGAG
    GTGTACTATG ACCACCTGCG CAGTGCCGCC AAGAAGAGGT TTCTGAGCTT CACTATGGTG
    ACTGAGAGCC CGAACCCGGC CCTCACCTTC TGCGTGAAGA CCCACGACCG GCTGTACTAC
    ATGGTGGCCC CCTCTGCAGA GGCCATGCGC ATCTGGATGG ACGTCATTGT CACGGGGGCA
    GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216064.1
    CDS393..4439
    Translation

    Target ORF information:

    RefSeq Version XM_005216007.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216007.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAG
    ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC
    TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA
    CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC
    ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGT CGAGTCGCTT
    CCTGCCGAGC CCCTCCCAAC CGACGACCCA GCAGGGCAGC AGGTGATCGA GGAGCAGCGG
    CGGCGGCTGG CGGAGCTGAA GCAGAAGGCC GCGGCTGAGG CACAGTGCCA GTGGGATGCC
    CTGCACGGGG TGGCACCCTT TCCCACCGGG CCCGCGGGCT TCCCGCCACT CATGCACCAC
    TCCATCCTGC ACCACCTGCC GGCCTCGCGG GAGCGAGGCG AGGAGGGTGA GCACGCCTAC
    GACACGCTGA GCCTGGAGAG CTCCGACAGC ATGGAGACCA GCATCTCCAC GGGGGGCAAC
    TCAGTCTGCT CCCCTGACAA CATGTCCAGT GCTAGCGGCC TGGACATGGG GAAGATTGAG
    GAAATGGAGA AGATGCTGAA AGAAGCTCAC GCGGAGAAGA GCCGACTGAT GGAGTCGAGG
    GAACGTGAGA TGGAGCTGAG GCGGCAGGCC CTGGAGGAGG AACGGAGGCG GCGGGAGCAG
    GTTGAAAGGA GGTTGCAGAG CGAGAGCGCC CGGAGGCAGC AGCTTGTGGA GAAGGAGGTC
    AAGATGCGGG AGAAGCAGTT CTCACAGGCA CGACCCCTGA CCCGTTACCT GCCAATCCGG
    AAGGAAGACT TTGACCTGAA GACCCACATC GAGTCGTCAG GCCATGGTGT GGATACCTGT
    CTGCACGTGG TGCTCAGCAG CAAGGTCTGC CGTGGCTACT TGGTCAAGAT GGGCGGCAAG
    ATCAAGTCGT GGAAGAAGCG CTGGTTTGTC TTCGACCGGC TCAAGCGCAC CCTTTCCTAT
    TACGTGGACA AGCATGAGAC GAAGCTGAAG GGGGTCATCT ACTTCCAGGC CATCGAGGAG
    GTGTACTATG ACCACCTGCG CAGTGCCGCC AAGAAGAGGT TTCTGAGCTT CACTATGGTG
    ACTGAGAGCC CGAACCCGGC CCTCACCTTC TGCGTGAAGA CCCACGACCG GCTGTACTAC
    ATGGTGGCCC CCTCTGCAGA GGCCATGCGC ATCTGGATGG ACGTCATTGT CACGGGGGCA
    GAGGGCTACA CTCAGTTTAT GAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa26361
    Clone ID Related Accession (Same CDS sequence) XM_005216008.3
    Accession Version XM_005216008.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216008.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAG
    ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC
    TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA
    CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC
    ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG
    ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG CTGAAGCAGA AGGCCGCGGC TGAGGCACAG
    TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG
    CCACTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG
    GGTGAGCACG CCTACGACAC GCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC
    TCCACGGGGG GCAACTCAGT CTGCTCCCCT GACAACATGT CCAGTGCTAG CGGCCTGGAC
    ATGGGGAAGA TTGAGGAAAT GGAGAAGATG CTGAAAGAAG CTCACGCGGA GAAGAGCCGA
    CTGATGGAGT CGAGGGAACG TGAGATGGAG CTGAGGCGGC AGGCCCTGGA GGAGGAACGG
    AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT
    GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG CAGTTCTCAC AGGCACGACC CCTGACCCGT
    TACCTGCCAA TCCGGAAGGA AGACTTTGAC CTGAAGACCC ACATCGAGTC GTCAGGCCAT
    GGTGTGGATA CCTGTCTGCA CGTGGTGCTC AGCAGCAAGG TCTGCCGTGG CTACTTGGTC
    AAGATGGGCG GCAAGATCAA GTCGTGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG
    CGCACCCTTT CCTATTACGT GGACAAGCAT GAGACGAAGC TGAAGGGGGT CATCTACTTC
    CAGGCCATCG AGGAGGTGTA CTATGACCAC CTGCGCAGTG CCGCCAAGAG CCCGAACCCG
    GCCCTCACCT TCTGCGTGAA GACCCACGAC CGGCTGTACT ACATGGTGGC CCCCTCTGCA
    GAGGCCATGC GCATCTGGAT GGACGTCATT GTCACGGGGG CAGAGGGCTA CACTCAGTTT
    ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005216065.1
    CDS392..4360
    Translation

    Target ORF information:

    RefSeq Version XM_005216008.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005216008.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGTTTA CCGCTCCAAG
    ATGGATGGCG AGGCCACCAG CCCCCTGCCC CGCACCCGCA GTGGGCCACT CCCCTCCTCC
    TCTGGCTCGT CGTCTTCCTC CTCCCAGCTC AGCGTGGCTA CCTTGGGCCG CAGCCCCTCA
    CCCAAGAGCA CTCTGCTCGC CCAGAACGGC ACCAGCAGCC TTCCTCGCAA CCTGGCAGCC
    ACACTGCAGG ACATCGAGAC CAAGCGCCAG CTGGCCCTGC AGCAGAAGGG GCAGCAGGTG
    ATCGAGGAGC AGCGGCGGCG GCTGGCGGAG CTGAAGCAGA AGGCCGCGGC TGAGGCACAG
    TGCCAGTGGG ATGCCCTGCA CGGGGTGGCA CCCTTTCCCA CCGGGCCCGC GGGCTTCCCG
    CCACTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCCT CGCGGGAGCG AGGCGAGGAG
    GGTGAGCACG CCTACGACAC GCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC
    TCCACGGGGG GCAACTCAGT CTGCTCCCCT GACAACATGT CCAGTGCTAG CGGCCTGGAC
    ATGGGGAAGA TTGAGGAAAT GGAGAAGATG CTGAAAGAAG CTCACGCGGA GAAGAGCCGA
    CTGATGGAGT CGAGGGAACG TGAGATGGAG CTGAGGCGGC AGGCCCTGGA GGAGGAACGG
    AGGCGGCGGG AGCAGGTTGA AAGGAGGTTG CAGAGCGAGA GCGCCCGGAG GCAGCAGCTT
    GTGGAGAAGG AGGTCAAGAT GCGGGAGAAG CAGTTCTCAC AGGCACGACC CCTGACCCGT
    TACCTGCCAA TCCGGAAGGA AGACTTTGAC CTGAAGACCC ACATCGAGTC GTCAGGCCAT
    GGTGTGGATA CCTGTCTGCA CGTGGTGCTC AGCAGCAAGG TCTGCCGTGG CTACTTGGTC
    AAGATGGGCG GCAAGATCAA GTCGTGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG
    CGCACCCTTT CCTATTACGT GGACAAGCAT GAGACGAAGC TGAAGGGGGT CATCTACTTC
    CAGGCCATCG AGGAGGTGTA CTATGACCAC CTGCGCAGTG CCGCCAAGAG CCCGAACCCG
    GCCCTCACCT TCTGCGTGAA GACCCACGAC CGGCTGTACT ACATGGTGGC CCCCTCTGCA
    GAGGCCATGC GCATCTGGAT GGACGTCATT GTCACGGGGG CAGAGGGCTA CACTCAGTTT
    ATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52569
    Clone ID Related Accession (Same CDS sequence) XM_010812483.2
    Accession Version XM_010812483.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAGCCCGAAC CCGGCCCTCA CCTTCTGCGT GAAGACCCAC
    GACCGGCTGT ACTACATGGT GGCCCCCTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC
    ATTGTCACGG GGGCAGAGGG CTACACTCAG TTTATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810785.1
    CDS395..4996
    Translation

    Target ORF information:

    RefSeq Version XM_010812483.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812483.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAGCCCGAAC CCGGCCCTCA CCTTCTGCGT GAAGACCCAC
    GACCGGCTGT ACTACATGGT GGCCCCCTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC
    ATTGTCACGG GGGCAGAGGG CTACACTCAG TTTATGAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52570
    Clone ID Related Accession (Same CDS sequence) XM_010812484.2
    Accession Version XM_010812484.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG
    CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT
    GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC
    CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC
    TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC
    AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT
    GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG
    AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG
    CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG
    GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC
    CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC
    TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC
    AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC
    TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG
    GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAAGA GGTTTCTGAG CTTCACTATG
    GTGACTGAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC
    TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG
    GCAGAGGGCT ACACTCAGTT TATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810786.1
    CDS395..4984
    Translation

    Target ORF information:

    RefSeq Version XM_010812484.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812484.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGGC AGCAGGTGAT CGAGGAGCAG
    CGGCGGCGGC TGGCGGAGCT GAAGCAGAAG GCCGCGGCTG AGGCACAGTG CCAGTGGGAT
    GCCCTGCACG GGGTGGCACC CTTTCCCACC GGGCCCGCGG GCTTCCCGCC ACTCATGCAC
    CACTCCATCC TGCACCACCT GCCGGCCTCG CGGGAGCGAG GCGAGGAGGG TGAGCACGCC
    TACGACACGC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACGGGGGGC
    AACTCAGTCT GCTCCCCTGA CAACATGTCC AGTGCTAGCG GCCTGGACAT GGGGAAGATT
    GAGGAAATGG AGAAGATGCT GAAAGAAGCT CACGCGGAGA AGAGCCGACT GATGGAGTCG
    AGGGAACGTG AGATGGAGCT GAGGCGGCAG GCCCTGGAGG AGGAACGGAG GCGGCGGGAG
    CAGGTTGAAA GGAGGTTGCA GAGCGAGAGC GCCCGGAGGC AGCAGCTTGT GGAGAAGGAG
    GTCAAGATGC GGGAGAAGCA GTTCTCACAG GCACGACCCC TGACCCGTTA CCTGCCAATC
    CGGAAGGAAG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CAGGCCATGG TGTGGATACC
    TGTCTGCACG TGGTGCTCAG CAGCAAGGTC TGCCGTGGCT ACTTGGTCAA GATGGGCGGC
    AAGATCAAGT CGTGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC
    TATTACGTGG ACAAGCATGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG
    GAGGTGTACT ATGACCACCT GCGCAGTGCC GCCAAGAAGA GGTTTCTGAG CTTCACTATG
    GTGACTGAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC
    TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG
    GCAGAGGGCT ACACTCAGTT TATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52571
    Clone ID Related Accession (Same CDS sequence) XM_010812485.2
    Accession Version XM_010812485.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGCTGA TCTGCTCATC
    TCCGAGTCCT CAGAGGTGGG GCTGGGGACG GCAGCTCTGG GCCCCTTCCC AGAGTCTTCT
    CAGGCTGGGG CCTCCTCTCT TCCCTTAACC CCACCTGCTT CCACTCAGCT CTGCCTCAAA
    GCACAAGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC GCCAGTGCCC
    ACAGGCCCCG CGCCGGGCCT GCCTCCCTGG GCCCCTGCCT CCCAGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGCGCT GCCCTCTTCC TCCTCCTTCG CTTCTGTCAC GCCTTCGCCC
    CAGATGGAGA AGCTGCTGCT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACGGGCCC TGCTGCCGCC TCCCCTCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGCCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGCGA GGCCACCAGC
    CCCCTGCCCC GCACCCGCAG TGGGCCACTC CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC
    TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC
    CAGAACGGCA CCAGCAGCCT TCCTCGCAAC CTGGCAGCCA CACTGCAGGA CATCGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC GAGTCGCTTC CTGCCGAGCC CCTCCCAACC
    GACGACCCAG CAGGGCAGCA GGTGATCGAG GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG
    CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG TGGGATGCCC TGCACGGGGT GGCACCCTTT
    CCCACCGGGC CCGCGGGCTT CCCGCCACTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG CACGCCTACG ACACGCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACG GGGGGCAACT CAGTCTGCTC CCCTGACAAC
    ATGTCCAGTG CTAGCGGCCT GGACATGGGG AAGATTGAGG AAATGGAGAA GATGCTGAAA
    GAAGCTCACG CGGAGAAGAG CCGACTGATG GAGTCGAGGG AACGTGAGAT GGAGCTGAGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC
    GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG AAGGAGGTCA AGATGCGGGA GAAGCAGTTC
    TCACAGGCAC GACCCCTGAC CCGTTACCTG CCAATCCGGA AGGAAGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCAGG CCATGGTGTG GATACCTGTC TGCACGTGGT GCTCAGCAGC
    AAGGTCTGCC GTGGCTACTT GGTCAAGATG GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ACGTGGACAA GCATGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAGG TGTACTATGA CCACCTGCGC
    AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC ACTATGGTGA CTGAGAGCCC GAACCCGGCC
    CTCACCTTCT GCGTGAAGAC CCACGACCGG CTGTACTACA TGGTGGCCCC CTCTGCAGAG
    GCCATGCGCA TCTGGATGGA CGTCATTGTC ACGGGGGCAG AGGGCTACAC TCAGTTTATG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810787.1
    CDS395..4900
    Translation

    Target ORF information:

    RefSeq Version XM_010812485.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812485.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGGCTGA TCTGCTCATC
    TCCGAGTCCT CAGAGGTGGG GCTGGGGACG GCAGCTCTGG GCCCCTTCCC AGAGTCTTCT
    CAGGCTGGGG CCTCCTCTCT TCCCTTAACC CCACCTGCTT CCACTCAGCT CTGCCTCAAA
    GCACAAGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC GCCAGTGCCC
    ACAGGCCCCG CGCCGGGCCT GCCTCCCTGG GCCCCTGCCT CCCAGGACCT GGTTCCCACC
    ACCTGCCTTC CTCCCGCGCT GCCCTCTTCC TCCTCCTTCG CTTCTGTCAC GCCTTCGCCC
    CAGATGGAGA AGCTGCTGCT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG
    GCCGGGCTGG GGACGGGCCC TGCTGCCGCC TCCCCTCGCT CCTCCCCCCC GCCTCTGCCC
    GCCAAAGCTT CCCGCCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGCGA GGCCACCAGC
    CCCCTGCCCC GCACCCGCAG TGGGCCACTC CCCTCCTCCT CTGGCTCGTC GTCTTCCTCC
    TCCCAGCTCA GCGTGGCTAC CTTGGGCCGC AGCCCCTCAC CCAAGAGCAC TCTGCTCGCC
    CAGAACGGCA CCAGCAGCCT TCCTCGCAAC CTGGCAGCCA CACTGCAGGA CATCGAGACC
    AAGCGCCAGC TGGCCCTGCA GCAGAAGGTC GAGTCGCTTC CTGCCGAGCC CCTCCCAACC
    GACGACCCAG CAGGGCAGCA GGTGATCGAG GAGCAGCGGC GGCGGCTGGC GGAGCTGAAG
    CAGAAGGCCG CGGCTGAGGC ACAGTGCCAG TGGGATGCCC TGCACGGGGT GGCACCCTTT
    CCCACCGGGC CCGCGGGCTT CCCGCCACTC ATGCACCACT CCATCCTGCA CCACCTGCCG
    GCCTCGCGGG AGCGAGGCGA GGAGGGTGAG CACGCCTACG ACACGCTGAG CCTGGAGAGC
    TCCGACAGCA TGGAGACCAG CATCTCCACG GGGGGCAACT CAGTCTGCTC CCCTGACAAC
    ATGTCCAGTG CTAGCGGCCT GGACATGGGG AAGATTGAGG AAATGGAGAA GATGCTGAAA
    GAAGCTCACG CGGAGAAGAG CCGACTGATG GAGTCGAGGG AACGTGAGAT GGAGCTGAGG
    CGGCAGGCCC TGGAGGAGGA ACGGAGGCGG CGGGAGCAGG TTGAAAGGAG GTTGCAGAGC
    GAGAGCGCCC GGAGGCAGCA GCTTGTGGAG AAGGAGGTCA AGATGCGGGA GAAGCAGTTC
    TCACAGGCAC GACCCCTGAC CCGTTACCTG CCAATCCGGA AGGAAGACTT TGACCTGAAG
    ACCCACATCG AGTCGTCAGG CCATGGTGTG GATACCTGTC TGCACGTGGT GCTCAGCAGC
    AAGGTCTGCC GTGGCTACTT GGTCAAGATG GGCGGCAAGA TCAAGTCGTG GAAGAAGCGC
    TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ACGTGGACAA GCATGAGACG
    AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAGG TGTACTATGA CCACCTGCGC
    AGTGCCGCCA AGAAGAGGTT TCTGAGCTTC ACTATGGTGA CTGAGAGCCC GAACCCGGCC
    CTCACCTTCT GCGTGAAGAC CCACGACCGG CTGTACTACA TGGTGGCCCC CTCTGCAGAG
    GCCATGCGCA TCTGGATGGA CGTCATTGTC ACGGGGGCAG AGGGCTACAC TCAGTTTATG
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52572
    Clone ID Related Accession (Same CDS sequence) XM_010812486.1
    Accession Version XM_010812486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810788.1
    CDS148..4422
    Translation

    Target ORF information:

    RefSeq Version XM_010812486.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG 
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAT
    CTGCTCATCT CCGAGTCCTC AGAGGTGGGG CTGGGGACGG CAGCTCTGGG CCCCTTCCCA
    GAGTCTTCTC AGGCTGGGGC CTCCTCTCTT CCCTTAACCC CACCTGCTTC CACTCAGCTC
    TGCCTCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCG
    CCAGTGCCCA CAGGCCCCGC GCCGGGCCTG CCTCCCTGGG CCCCTGCCTC CCAGGACCTG
    GTTCCCACCA CCTGCCTTCC TCCCGCGCTG CCCTCTTCCT CCTCCTTCGC TTCTGTCACG
    CCTTCGCCCC AGATGGAGAA GCTGCTGCTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG
    GAGCTGATGG CCGGGCTGGG GACGGGCCCT GCTGCCGCCT CCCCTCGCTC CTCCCCCCCG
    CCTCTGCCCG CCAAAGCTTC CCGCCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGCGAG
    GCCACCAGCC CCCTGCCCCG CACCCGCAGT GGGCCACTCC CCTCCTCCTC TGGCTCGTCG
    TCTTCCTCCT CCCAGCTCAG CGTGGCTACC TTGGGCCGCA GCCCCTCACC CAAGAGCACT
    CTGCTCGCCC AGAACGGCAC CAGCAGCCTT CCTCGCAACC TGGCAGCCAC ACTGCAGGAC
    ATCGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGTCG AGTCGCTTCC TGCCGAGCCC
    CTCCCAACCG ACGACCCAGC AGGGCAGCAG GTGATCGAGG AGCAGCGGCG GCGGCTGGCG
    GAGCTGAAGC AGAAGGCCGC GGCTGAGGCA CAGTGCCAGT GGGATGCCCT GCACGGGGTG
    GCACCCTTTC CCACCGGGCC CGCGGGCTTC CCGCCACTCA TGCACCACTC CATCCTGCAC
    CACCTGCCGG CCTCGCGGGA GCGAGGCGAG GAGGGTGAGC ACGCCTACGA CACGCTGAGC
    CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACGG GGGGCAACTC AGTCTGCTCC
    CCTGACAACA TGTCCAGTGC TAGCGGCCTG GACATGGGGA AGATTGAGGA AATGGAGAAG
    ATGCTGAAAG AAGCTCACGC GGAGAAGAGC CGACTGATGG AGTCGAGGGA ACGTGAGATG
    GAGCTGAGGC GGCAGGCCCT GGAGGAGGAA CGGAGGCGGC GGGAGCAGGT TGAAAGGAGG
    TTGCAGAGCG AGAGCGCCCG GAGGCAGCAG CTTGTGGAGA AGGAGGTCAA GATGCGGGAG
    AAGCAGTTCT CACAGGCACG ACCCCTGACC CGTTACCTGC CAATCCGGAA GGAAGACTTT
    GACCTGAAGA CCCACATCGA GTCGTCAGGC CATGGTGTGG ATACCTGTCT GCACGTGGTG
    CTCAGCAGCA AGGTCTGCCG TGGCTACTTG GTCAAGATGG GCGGCAAGAT CAAGTCGTGG
    AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA CGTGGACAAG
    CATGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAGGT GTACTATGAC
    CACCTGCGCA GTGCCGCCAA GAAGAGGTTT CTGAGCTTCA CTATGGTGAC TGAGAGCCCG
    AACCCGGCCC TCACCTTCTG CGTGAAGACC CACGACCGGC TGTACTACAT GGTGGCCCCC
    TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATTGTCA CGGGGGCAGA GGGCTACACT
    CAGTTTATGA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52573
    Clone ID Related Accession (Same CDS sequence) XM_010812487.2
    Accession Version XM_010812487.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC
    TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG
    GCAGAGGGCT ACACTCAGTT TATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810789.1
    CDS393..4502
    Translation

    Target ORF information:

    RefSeq Version XM_010812487.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010812487.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GGCCGAGGCC
    CTGGAGACGG AGACGAAGCT CTTCGAGGAC TTGGAGTTCC AGCAGCTGGA GCGGGAGAGC
    CGCGTGGAGG AGGAGCGAGA GCTGGCGGGC CAGGGACTGC TCCGGAGCAA GGCCGAACTG
    CTCCGCAGCA TCGCCAAGAG GAAGGAGCGC CTGGTGGTCC TGGACAGTCA GGCTGGACAG
    ATCCGGTCCC AGGCTGTGCA GGAGTCTGAA CACCTAGCCC GGGATAAGAA TGCTGCCCTG
    CAGCTGCTGC AGAAGGAGAA GGAGAAACTG GCTATGCTGG AAAGAAGATA CCACTCGCTT
    ACAGGCGGCC GGCCTTTCCC GAAGACCACG TCCACCCTCA AAGAGATGGA GAAGCTGCTG
    CTCCCTGCTG TAGACTTAGA GCAGTGGTAC CAGGAGCTGA TGGCCGGGCT GGGGACGGGC
    CCTGCTGCCG CCTCCCCTCG CTCCTCCCCC CCGCCTCTGC CCGCCAAAGC TTCCCGCCAG
    CTGCAGGTTT ACCGCTCCAA GATGGATGGC GAGGCCACCA GCCCCCTGCC CCGCACCCGC
    AGTGGGCCAC TCCCCTCCTC CTCTGGCTCG TCGTCTTCCT CCTCCCAGCT CAGCGTGGCT
    ACCTTGGGCC GCAGCCCCTC ACCCAAGAGC ACTCTGCTCG CCCAGAACGG CACCAGCAGC
    CTTCCTCGCA ACCTGGCAGC CACACTGCAG GACATCGAGA CCAAGCGCCA GCTGGCCCTG
    CAGCAGAAGG GGCAGCAGGT GATCGAGGAG CAGCGGCGGC GGCTGGCGGA GCTGAAGCAG
    AAGGCCGCGG CTGAGGCACA GTGCCAGTGG GATGCCCTGC ACGGGGTGGC ACCCTTTCCC
    ACCGGGCCCG CGGGCTTCCC GCCACTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCC
    TCGCGGGAGC GAGGCGAGGA GGGTGAGCAC GCCTACGACA CGCTGAGCCT GGAGAGCTCC
    GACAGCATGG AGACCAGCAT CTCCACGGGG GGCAACTCAG TCTGCTCCCC TGACAACATG
    TCCAGTGCTA GCGGCCTGGA CATGGGGAAG ATTGAGGAAA TGGAGAAGAT GCTGAAAGAA
    GCTCACGCGG AGAAGAGCCG ACTGATGGAG TCGAGGGAAC GTGAGATGGA GCTGAGGCGG
    CAGGCCCTGG AGGAGGAACG GAGGCGGCGG GAGCAGGTTG AAAGGAGGTT GCAGAGCGAG
    AGCGCCCGGA GGCAGCAGCT TGTGGAGAAG GAGGTCAAGA TGCGGGAGAA GCAGTTCTCA
    CAGGCACGAC CCCTGACCCG TTACCTGCCA ATCCGGAAGG AAGACTTTGA CCTGAAGACC
    CACATCGAGT CGTCAGGCCA TGGTGTGGAT ACCTGTCTGC ACGTGGTGCT CAGCAGCAAG
    GTCTGCCGTG GCTACTTGGT CAAGATGGGC GGCAAGATCA AGTCGTGGAA GAAGCGCTGG
    TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTACG TGGACAAGCA TGAGACGAAG
    CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAGGTGT ACTATGACCA CCTGCGCAGT
    GCCGCCAAGA GCCCGAACCC GGCCCTCACC TTCTGCGTGA AGACCCACGA CCGGCTGTAC
    TACATGGTGG CCCCCTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT TGTCACGGGG
    GCAGAGGGCT ACACTCAGTT TATGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92656
    Clone ID Related Accession (Same CDS sequence) XM_015474658.1
    Accession Version XM_015474658.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4491bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pleckstrin homology-like domain family B member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104409.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGTACGTG
    ACGCTTGAGC AGCTAAAGGT GATGTGGGGC ACCTCGCCAG TGCCCACAGG CCCCGCGCCG
    GGCCTGCCTC CCTGGGCCCC TGCCTCCCAG GACCTGGTTC CCACCACCTG CCTTCCTCCC
    GCGCTGCCCT CTTCCTCCTC CTTCGCTTCT GTCACGCCTT CGCCCCAGAT GGAGAAGCTG
    CTGCTCCCTG CTGTAGACTT AGAGCAGTGG TACCAGGAGC TGATGGCCGG GCTGGGGACG
    GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC
    CAGCTGCAGG TTTACCGCTC CAAGATGGAT GGCGAGGCCA CCAGCCCCCT GCCCCGCACC
    CGCAGTGGGC CACTCCCCTC CTCCTCTGGC TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG
    GCTACCTTGG GCCGCAGCCC CTCACCCAAG AGCACTCTGC TCGCCCAGAA CGGCACCAGC
    AGCCTTCCTC GCAACCTGGC AGCCACACTG CAGGACATCG AGACCAAGCG CCAGCTGGCC
    CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC GAGCCCCTCC CAACCGACGA CCCAGCAGGG
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT
    GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG
    GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA
    GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC
    GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG
    AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG
    CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC
    CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC
    TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG
    AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG
    AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330144.1
    CDS395..4885
    Translation

    Target ORF information:

    RefSeq Version XM_015474658.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGACACTC TCAATAGGAA CCAGGTGGGC CCTGGATGTA AGACCCCGGG CCTGGTGCAG 
    AAAGGACCCT TGGACCTGAT CGAAACAGGC AAAGGGCTGA AAGTGCAGAC GGACAAACCC
    CATCTGGTGA GCCTGGGCAG CGGGCGGCTC AGCACAGCCA TCACCCTTCT GCCACTGGAG
    GAAGGGAGGA CCGTGATTGG CTCTGCAGCC AGGGACATCT CACTGCAGGG TCCAGGCCTG
    GCTCCGGAGC ACTGCTACAT CGAGAACCTG CGGGGCACCC TCACCCTCTA CCCCTGCGGC
    AATGCCTGCA CTATTGATGG GCTCCCGGTC CGGCAGCCCA CTCGGCTCAC TCAGGGCTGC
    ATGTTGTGTC TGGGTCAGTC CACCTTCCTC CGCTTTAACC ACCCAGCTGA AGCCAAGTGG
    ATGAAGAGCA TGATCCCGGC AGGGGGCCGG GCCCCTGGCC CCCCGTATAG TCCTGGCCCA
    GAATCAGAAA GCCTGGTGAA CGGGAACCAT ACCCCGCAGC ATGCAAGCCG GGGACCCTCT
    GCCTGTGGCA GCCACAGTTC CCTGGTGAGC TCTATTGAGA AGGACCTGCA GGAAATCATG
    GACTCACTGG TGCTAGAGGA GCCTGGAGCT GCTGGCAAGA AGCCTGCCGC AACATCTCCA
    CTATCACCAA TGGCCAATGG TGGGCGCTAC CTGCTGTCCC CCCCAACCAG CCCGGGTGCC
    ATGTCTGTGG GCTCCAGCTA TGAGAACACT TCTCCAGCCT TCTCTCCACT CTCCTCGCCA
    GCCAGTAGTG GGAGCTGTGC CAGCCACTCA CCCAGTGGGC AGGAACCAGC ACCTTCCCTG
    CCCCCCCTGG TGCCCGCCCG GTCCTCCAGC TACCATCTGG GCCTGCAGCC CCCACAGTCC
    CGACCGAGCG GCGCTCGCCC CTCTGAGAGC CCTCGGCTGG GCAGGAAGGG GGGTCACGAG
    AGGCCTCCCA GCCCTGGTCT CCGAGGTCTG CTGACAGACA GCCCTGCAGC CACCGTCCTG
    GCCGAGGCCC GCAGAGCCAC CGAGAGCCCC CGGCCAGGTG GGCAGCTGCC CGTGGTGGCG
    ATCAGTCTGA GTGAATACCC AGCTTCCGGT GCCCGGGGCC CGCCCACCAG CATTCCTGGC
    AGCCCCAAGT TTCAGCCGCC AGTCCCTGCT CCCCGAAACA AGATTGGCAC GCTCCAGGAC
    CGCCCTCCTA GCCCTTTCCG GGAGCTGCCA GGCGCTGAGC GGGTGCTGAC CACCAGCCCC
    TCACGCCAGC TGGTAGGCCG AACGTTTTCG GATGGCTCAG CCACACGCAC CCTGCAGCCT
    CCAGAGAGCC CCCGCCTAGG CCGGCGGGGC CTGGACAGCA TGAGAGAACT GCCTCCCTTG
    AGCCCATCTC TGTCCCGCAG GGCTCTCTCC CCCGTGTCCA CCCGGACTGC CCCAGATCCC
    AAACTAACCA GGGAAGTGGC AGAAAGCCCC CGCCCCCGGC GCTGGGCAGC CCACGGGGCT
    TCACCGGAGG ACTTCTCCCT GACACTGGGG GCCCGGGGCC GTAGGACACG GAGCCCCTCG
    CCCACACTCG GGGAGTCCCT AGCACCCCGC AAGGGCAGCT TCAGCGGCAG GCTGAGCCCG
    GCTTACAGCC TGGGCTCCTT GACTGGGGCT TCACCCCGCC AGAGCCCCCG CGCCCAGAGG
    AAGCTCTCCA GCGGGGACCT GCGGGTGCCT GTCACCCGGG AGCGGAAAAA CAGCATCACG
    GAGATCAGTG ACAATGAGGA TGACCTCCTG GAGTACCACC GGCGGCAGCG CCAAGAGCGG
    CTTCGGGAGC AGGAGATGGA GAGGCTGGAA CGCCAGCGCC TGGAGACCAT CCTGAACCTG
    TGCGCCGAGT ACAGCCGGGC TGACGGGGGA CCTGAGGCCG GGGAGCTGCC TAGCATAGGG
    GAGGCGGCCG CAGCCCTGGC CCTGGCCGGC CGGAGGCCCT CACGAGGCCT CGCGGCGGGC
    ACTGGGGCCC CGGGGCGCGG CAGCGAGGAG CCTGGAGGCG CCGCCCAGCG CCTGTGGGAG
    AGTGTGGAGC GCTCAGACGA GGAGAATCTC AAGGAGGAGT GCAGCAGCAC TGAGAGCACC
    CAGCAGGAGC ACGAGGATGC CCCCGGCACC AAGCTCCAGG GAGAGGTGCT GGCCCTGGAA
    GAGGAGCGGG CTCAGGTGCT GGGGCGAGTA GAGCAGCTCA AAGTCCGGGT GAAGGAGCTG
    GAGCAGCAGC TGCAGGAGTC GGCCCGAGAG GCTGAAATGG AGCGGGCGCT TCTGCAGGGG
    GAGAGGGAGG CCGAGCGGGC GCTGCTGCAG AAAGAGCAGA AGGCGGTGGA CCAGCTGCAG
    GAGAAGCTGG TGACCTTGGA GACGAGCATC CAGAAGGAGA GGGACAAGGA GAGGGCGGAG
    CTGGCCGCGG GACGGAGGCA CCTGGAGGCC CGCCAGGCGC TCTACGCCGA GCTCCAGACG
    CAGCTCGATA ACTGCCCCGA GTCAGTGCGG GAACAGTTAC AGGAGCAGCT GAGAAGGGAG
    GCCGAGGCCC TGGAGACGGA GACGAAGCTC TTCGAGGACT TGGAGTTCCA GCAGCTGGAG
    CGGGAGAGCC GCGTGGAGGA GGAGCGAGAG CTGGCGGGCC AGGGACTGCT CCGGAGCAAG
    GCCGAACTGC TCCGCAGCAT CGCCAAGAGG AAGGAGCGCC TGGTGGTCCT GGACAGTCAG
    GCTGGACAGA TCCGGTCCCA GGCTGTGCAG GAGTCTGAAC ACCTAGCCCG GGATAAGAAT
    GCTGCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAAACTGG CTATGCTGGA AAGAAGATAC
    CACTCGCTTA CAGGCGGCCG GCCTTTCCCG AAGACCACGT CCACCCTCAA AGAGTACGTG
    ACGCTTGAGC AGCTAAAGGT GATGTGGGGC ACCTCGCCAG TGCCCACAGG CCCCGCGCCG
    GGCCTGCCTC CCTGGGCCCC TGCCTCCCAG GACCTGGTTC CCACCACCTG CCTTCCTCCC
    GCGCTGCCCT CTTCCTCCTC CTTCGCTTCT GTCACGCCTT CGCCCCAGAT GGAGAAGCTG
    CTGCTCCCTG CTGTAGACTT AGAGCAGTGG TACCAGGAGC TGATGGCCGG GCTGGGGACG
    GGCCCTGCTG CCGCCTCCCC TCGCTCCTCC CCCCCGCCTC TGCCCGCCAA AGCTTCCCGC
    CAGCTGCAGG TTTACCGCTC CAAGATGGAT GGCGAGGCCA CCAGCCCCCT GCCCCGCACC
    CGCAGTGGGC CACTCCCCTC CTCCTCTGGC TCGTCGTCTT CCTCCTCCCA GCTCAGCGTG
    GCTACCTTGG GCCGCAGCCC CTCACCCAAG AGCACTCTGC TCGCCCAGAA CGGCACCAGC
    AGCCTTCCTC GCAACCTGGC AGCCACACTG CAGGACATCG AGACCAAGCG CCAGCTGGCC
    CTGCAGCAGA AGGTCGAGTC GCTTCCTGCC GAGCCCCTCC CAACCGACGA CCCAGCAGGG
    CAGCAGGTGA TCGAGGAGCA GCGGCGGCGG CTGGCGGAGC TGAAGCAGAA GGCCGCGGCT
    GAGGCACAGT GCCAGTGGGA TGCCCTGCAC GGGGTGGCAC CCTTTCCCAC CGGGCCCGCG
    GGCTTCCCGC CACTCATGCA CCACTCCATC CTGCACCACC TGCCGGCCTC GCGGGAGCGA
    GGCGAGGAGG GTGAGCACGC CTACGACACG CTGAGCCTGG AGAGCTCCGA CAGCATGGAG
    ACCAGCATCT CCACGGGGGG CAACTCAGTC TGCTCCCCTG ACAACATGTC CAGTGCTAGC
    GGCCTGGACA TGGGGAAGAT TGAGGAAATG GAGAAGATGC TGAAAGAAGC TCACGCGGAG
    AAGAGCCGAC TGATGGAGTC GAGGGAACGT GAGATGGAGC TGAGGCGGCA GGCCCTGGAG
    GAGGAACGGA GGCGGCGGGA GCAGGTTGAA AGGAGGTTGC AGAGCGAGAG CGCCCGGAGG
    CAGCAGCTTG TGGAGAAGGA GGTCAAGATG CGGGAGAAGC AGTTCTCACA GGCACGACCC
    CTGACCCGTT ACCTGCCAAT CCGGAAGGAA GACTTTGACC TGAAGACCCA CATCGAGTCG
    TCAGGCCATG GTGTGGATAC CTGTCTGCAC GTGGTGCTCA GCAGCAAGGT CTGCCGTGGC
    TACTTGGTCA AGATGGGCGG CAAGATCAAG TCGTGGAAGA AGCGCTGGTT TGTCTTCGAC
    CGGCTCAAGC GCACCCTTTC CTATTACGTG GACAAGCATG AGACGAAGCT GAAGGGGGTC
    ATCTACTTCC AGGCCATCGA GGAGGTGTAC TATGACCACC TGCGCAGTGC CGCCAAGAAG
    AGGTTTCTGA GCTTCACTAT GGTGACTGAG AGCCCGAACC CGGCCCTCAC CTTCTGCGTG
    AAGACCCACG ACCGGCTGTA CTACATGGTG GCCCCCTCTG CAGAGGCCAT GCGCATCTGG
    ATGGACGTCA TTGTCACGGG GGCAGAGGGC TACACTCAGT TTATGAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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