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CACNA1S cDNA ORF clone, Canis lupus familiaris(dog)

Summary

Gene Symbol CACNA1S
Entrez Gene ID 490244
Full Name calcium voltage-gated channel subunit alpha1 S
General protein information
Preferred Names

calcium voltage-gated channel subunit alpha1 S

Names

voltage-dependent L-type calcium channel subunit alpha-1S
Gene Type protein-coding
Organism Canis lupus familiaris(dog)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_843592.5 XP_848685.3 voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
XM_843592.6 XP_848685.3 voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
XM_005622266.2 XP_005622323.1 voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
XM_005622266.3 XP_005622323.1 voltage-dependent L-type calcium channel subunit alpha-1S isoform X2

Pathways from BioSystems


Homologies


CACNA1S XP_002802046.1
Rattus norvegicus (Norway rat) Cacna1s NP_446325.1
Homo sapiens (human) CACNA1S NP_000060.2
Pan troglodytes (chimpanzee) CACNA1S XP_525018.3
Canis lupus familiaris (dog) CACNA1S XP_848685.3
Bos taurus (cattle) CACNA1S XP_002694288.2
Mus musculus (house mouse) Cacna1s NP_001074492.1
Danio rerio (zebrafish) cacna1sa NP_001139622.1
Gallus gallus (chicken) CACNA1S XP_419259.3
Danio rerio (zebrafish) cacna1sb NP_999891.1

Gene Ontology

The following CACNA1S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe08962 XM_843592.6
Latest version!		 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08963 XM_005622266.3
Latest version!		 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OCe08962 XM_843592.5 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe08963 XM_005622266.2 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OCe08962
Clone ID Related Accession (Same CDS sequence) XM_843592.5 , XM_843592.6
Accession Version XM_843592.5 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5622bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_843592.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
ATGGAGCCGT CCTCGCCCCA GGACGAGGGC CTGAGGAAGA AACAGCCCAA GAAGCCGGTT 
CCGGAGATCC TGCCGAGGCC GCCCCGGGCC CTGTTCTGCT TGACCCTGCA GAACCCCCTG 
AGGAAGGCCT GCATCAACAT TGTGGAATGG AAACCCTTCG AGACAATCAT CTTGCTCACC 
ATCTTTGCCA ACTGCGTGGC TCTGGCTGTG TACCTGCCCA TGCCGGAAGA CGACAACAAC 
ACTCTCAACC TTGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTTTT CTCCATCGAA 
GCGGCCATGA AGATCATCGC CTACGGCTTC CTCTTCCACC AGGACGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGACTT CATCATTGTC TTCCTGGGGG TTTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CAACACAGCC CCGATGAGCA GCAAAGGGGC CGGCCTGGAT 
GTCAAGGCCC TGAGAGCCTT CCGTGTGCTC AGACCCCTCC GGCTGGTGTC AGGGGTGCCG 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCCCTGTT CCACATCGCC 
CTGCTCGTCC TCTTTATGGT CATCATCTAC GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGG ACAGATATCG TGGCCACGGT GGAGAACGAG 
AAGCCCTCAC CCTGTGCCAG GACGGGCTCG GGGCGCCCAT GCACCATCAA TGGCAGCGAG 
TGTCGGGGCG GCTGGCCGGG GCCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATCACC ATGGAGGGGT GGACCGATGT CCTCTACTGG 
GTCAACGATG CCATCGGGAA CGAGTGGCCC TGGATCTATT TTGTCACCCT CATTCTGCTG 
GGATCCTTCT TCATTCTCAA CCTGGTGCTG GGTGTCCTGA GCGGGGAATT CACTAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GCGGCTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTCGAGGAC 
TTGAGAGAAG GGAAGCTGTC TTTGGATGAA GGAGGCTCCG ACACAGAGAG CCTGTACGAG 
ATCGAGGGCC TGAACAAAAT CATCCAGTTC GTCCGCCACT GGAGGCAGTG GAACCGGATC 
TTTCGCTGGA AGTGCCATGA CGTGATCAAG TCCAGGGTCT TCTACTGGCT GGTGATCCTG 
GTCGTCGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC CCTGTGGCTG 
ACCCACTTGC AAGACGTGGC CAACCGGGTG CTGCTGGCGC TCTTCACCGT CGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTCG TGGAATCGGG CGCCATGAGC 
CCCCTGGGCA TCTCCGTGCT GCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAG 
TACTGGACGT CCCTGAGCAA CCTGGTGGCG TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC ATCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTATGACTT CGAGGACACC GAGGTGCGGC GCAGCAACTT TGACAACTTC 
CCGCAGGCCC TCATCAGCGT CTTCCAGGTC CTGACGGGCG AGGACTGGAA CTCCATGATG 
TACAGCGGGA TCATGGCCTA CGGCGGCCCG TCCTACCCGG GGGTGCTCGT GTGCATTTAT 
TTCATCATTC TGTTCGTCTG TGGCAACTAT ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCGGAGGC AGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCGGAGGAG 
AGAAAGCGCA GGAAGATGTC CAAGGGTCTC CCAGATAAGT CAGAGGAGGA GAAGTCCGTG 
ATGGCCAAGA AGCTAGAGCA GAAACCCAAG GGTGAGGGCA TCCCTACCAC CGCCAAGCTG 
AAGATCGATG AGTTTGAATC GAATGTCAAC GAAGTGAAGG ATCCTTACCC CTCGGCTGAC 
TTCCCAGGCG ATGATGAGGA AGACGAGCCT GAGATCCCAG TGAGCCCCCG ACCACGGCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCA GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGTGTC CTGTGCCACC GCATTGTCAA CGCTACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CCGCCCTGGC CGCTGAGGAC 
CCCATCCGGG CCGAGTCTGT GAGGAATCAG ATCCTTGGGT ATTTTGACAT CGCTTTCACC 
TCTGTTTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGCGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGCACCATC TCCGTGGTGA AAATCCTGAG GGTGCTCAGG 
GTGCTCCGAC CCCTGCGGGC CATCAACAGA GCCAAGGGGC TGAAGCACGT GGTGCAGTGC 
GTGTTCGTGG CCATCCGCAC GATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTA 
TCCAAGATGA CAGAGCAGGA GTGCAGGGGC TATTACTACG TGTACAAGGA CGGGGACCCC 
ACGCAGATGG AGCTGCGTCC CCGCCAGTGG ATACACAGCG ACTTCCACTT TGACAACGTG 
CTCTCCGCCA TGATGTCACT CTTCACAGTG TCCACCTTTG AGGGATGGCC CCAGCTACTT 
TACCAGGCCA TAGACTCCTA CAAGGAGGAC ATGGGCCCCG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTACAG TACGCCCTGA AGGCCCGCCC GCTGAGATGC 
TATATCCCCA AAAATCCATA CCAGTACCAG GTGTGGTACG TTGTCACCTC CTCCTACTTC 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCAG AGGAGATGAA CCACATCTCA GATATCCTCA ATGTGGCCTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAACTCATG GCATTCAAGG CCAGGGGCTA TTTTGGAGAC 
CCGTGGAACG TGTTCGACTT CTTGATTGTC ATCGGCAGCA TCATTGACGT CATCCTCAGT 
GAAATTGATA CTTTCCTGGC CTCCAGCGGA GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGACGAGAG CGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGGGCA GAGGGAGTCC GCACTCTCCT GTGGACGTTC 
ATCAAGTCCT TTCAGGCCCT GCCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCCGTCA TCGGCATGCA GATGTTTGGG AAGATCGCCA TGGTGGACGG GACCCAGATA 
AACCGGAACA ACAACTTCCA GACCTTCCCA CAAGCTGTGT TACTGCTCTT CAGGTGTGCG 
ACAGGGGAGG CGTGGCAGGA GATCTTGCTG GCCTGCAGCT ACGGCAAGTT GTGCGACCCC 
GAGTCAGACT ACGCCCCGGG CGAGGAGTAC ACGTGTGGCA CCAACTTCGC CTACTACTAT 
TTCATCAGCT TCTACATGCT GTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCTGTCATC 
ATGGACAACT TTGACTACCT CACGCGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATTTGGGC AGAGTATGAC CCAGAAGCCA AGGGCAGAAT CAAACACCTG 
GATGTGGTGA CGCTGCTGAG ACGCATCCAG CCCCCCCTGG GCTTTGGCAA GTTCTGCCCA 
CACCGGGTGG CCTGCAAGCG GCTGGTGGGC ATGAACATGC CCTTGAACAG CGACGGCACC 
GTCACCTTCA ACGCCACGCT CTTCGCGCTG GTCCGCACGG CCCTCAAGAT CAAGACGGAA 
GGTAACTTTG AGCAGGCCAA CGAGGAGCTG AGGGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCCTGGA CCAGGTCATT CCTCCAATAG GAGATGATGA GGTGACTGTA 
GGGAAGTTCT ACGCCACGTT TCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAAGAATATT ATGGGTATCG GCCCAAGAAG GACACCATAC AGATCCAGGC TGGACTGCGG 
ACCATTGAGG AAGAGGCAGC CCCAGAGATC CGCCGTACCA TCTCAGGAGA CCTGACAGCT 
GAAGAGGAGC TGGAGAGAGC CATGGTGGAG GCTGCGATGG AGGAAGGGAT ATTCCGGAGG 
ACCGGAGGCC TCTTTGGCCA GGTGGACAAC TTCCTGGAAA GGACCAACTC CCTGCCCCCT 
GTCATGGCCA ACCAGAGACC CCTCCAGTTT GCTGAGATAG AGATGGAAGA GCTGGAATCA 
CCCGTCTTCT TGGAGGATTT CCCTCAAGAT CCTAGAACCA ACCCCCTTGC TCGTGCCAAT 
ACCAACAATG CCAATGCCAA CGTTGCCCAT GACAACAGTG ACCATAGCAA CAGCCAGGTG 
TTTTCCAGCA TCCGCTACGA GAGGGAGTTC CCTGGAGAGC CAGAGCCGCC TGCTGCCAGA 
GGAGGAGCCT TGGGCCAACC CCGGAGGGTC CTGGGACCCC ACGGCAGGCC CTGTGTGGAA 
AAGCTGAAAG GACAGAGGAC CCAGAGAGAA ATGCCCAGAC CCCTGGCGTC TCCCGCCCCC 
TGCCAGTGTG CCGGGCCGGA GCGTCCCCTG CCCGAGGAGC GGAGTTGCCC CCCGCCGGGG 
TCTCTGCCTC AGGGAGCGCC CCCCAGCAGG AGGCCCACGG AGAGTGGCTG CAGGTGCCCG 
ACCCCAGCTA CGGCCCGGCT GGTTCGAGAG GCTCTGGTCC GAGGAGGCCT GGACACCTTG 
GCGGCCGATG CCAGCTTCGT CATGGCAACG GGCCAGGCCC TGGCAGATGC CTGCCGGATG 
GAACCCGAGG ACGTGGAGGT GGCGGCAACA GAGCTGCTCA AGAAGCAAGA GTCCCCAGAG 
GGCCGGGCCA GTGCGCTGGG ACGCCGGAGC CGCGGGTCCT CGCTGGGCAG CCTTGACCTG 
CATCTGGGCT CCCGGGAGAC CCTGGTGCCC CCCAGGCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_848685.3
CDS114..5735
Translation

Target ORF information:

RefSeq Version XM_843592.5
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_843592.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
ATGGAGCCGT CCTCGCCCCA GGACGAGGGC CTGAGGAAGA AACAGCCCAA GAAGCCGGTT 
CCGGAGATCC TGCCGAGGCC GCCCCGGGCC CTGTTCTGCT TGACCCTGCA GAACCCCCTG 
AGGAAGGCCT GCATCAACAT TGTGGAATGG AAACCCTTCG AGACAATCAT CTTGCTCACC 
ATCTTTGCCA ACTGCGTGGC TCTGGCTGTG TACCTGCCCA TGCCGGAAGA CGACAACAAC 
ACTCTCAACC TTGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTTTT CTCCATCGAA 
GCGGCCATGA AGATCATCGC CTACGGCTTC CTCTTCCACC AGGACGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGACTT CATCATTGTC TTCCTGGGGG TTTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CAACACAGCC CCGATGAGCA GCAAAGGGGC CGGCCTGGAT 
GTCAAGGCCC TGAGAGCCTT CCGTGTGCTC AGACCCCTCC GGCTGGTGTC AGGGGTGCCG 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCCCTGTT CCACATCGCC 
CTGCTCGTCC TCTTTATGGT CATCATCTAC GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGG ACAGATATCG TGGCCACGGT GGAGAACGAG 
AAGCCCTCAC CCTGTGCCAG GACGGGCTCG GGGCGCCCAT GCACCATCAA TGGCAGCGAG 
TGTCGGGGCG GCTGGCCGGG GCCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATCACC ATGGAGGGGT GGACCGATGT CCTCTACTGG 
GTCAACGATG CCATCGGGAA CGAGTGGCCC TGGATCTATT TTGTCACCCT CATTCTGCTG 
GGATCCTTCT TCATTCTCAA CCTGGTGCTG GGTGTCCTGA GCGGGGAATT CACTAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GCGGCTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTCGAGGAC 
TTGAGAGAAG GGAAGCTGTC TTTGGATGAA GGAGGCTCCG ACACAGAGAG CCTGTACGAG 
ATCGAGGGCC TGAACAAAAT CATCCAGTTC GTCCGCCACT GGAGGCAGTG GAACCGGATC 
TTTCGCTGGA AGTGCCATGA CGTGATCAAG TCCAGGGTCT TCTACTGGCT GGTGATCCTG 
GTCGTCGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC CCTGTGGCTG 
ACCCACTTGC AAGACGTGGC CAACCGGGTG CTGCTGGCGC TCTTCACCGT CGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTCG TGGAATCGGG CGCCATGAGC 
CCCCTGGGCA TCTCCGTGCT GCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAG 
TACTGGACGT CCCTGAGCAA CCTGGTGGCG TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC ATCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTATGACTT CGAGGACACC GAGGTGCGGC GCAGCAACTT TGACAACTTC 
CCGCAGGCCC TCATCAGCGT CTTCCAGGTC CTGACGGGCG AGGACTGGAA CTCCATGATG 
TACAGCGGGA TCATGGCCTA CGGCGGCCCG TCCTACCCGG GGGTGCTCGT GTGCATTTAT 
TTCATCATTC TGTTCGTCTG TGGCAACTAT ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCGGAGGC AGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCGGAGGAG 
AGAAAGCGCA GGAAGATGTC CAAGGGTCTC CCAGATAAGT CAGAGGAGGA GAAGTCCGTG 
ATGGCCAAGA AGCTAGAGCA GAAACCCAAG GGTGAGGGCA TCCCTACCAC CGCCAAGCTG 
AAGATCGATG AGTTTGAATC GAATGTCAAC GAAGTGAAGG ATCCTTACCC CTCGGCTGAC 
TTCCCAGGCG ATGATGAGGA AGACGAGCCT GAGATCCCAG TGAGCCCCCG ACCACGGCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCA GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGTGTC CTGTGCCACC GCATTGTCAA CGCTACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CCGCCCTGGC CGCTGAGGAC 
CCCATCCGGG CCGAGTCTGT GAGGAATCAG ATCCTTGGGT ATTTTGACAT CGCTTTCACC 
TCTGTTTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGCGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGCACCATC TCCGTGGTGA AAATCCTGAG GGTGCTCAGG 
GTGCTCCGAC CCCTGCGGGC CATCAACAGA GCCAAGGGGC TGAAGCACGT GGTGCAGTGC 
GTGTTCGTGG CCATCCGCAC GATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTA 
TCCAAGATGA CAGAGCAGGA GTGCAGGGGC TATTACTACG TGTACAAGGA CGGGGACCCC 
ACGCAGATGG AGCTGCGTCC CCGCCAGTGG ATACACAGCG ACTTCCACTT TGACAACGTG 
CTCTCCGCCA TGATGTCACT CTTCACAGTG TCCACCTTTG AGGGATGGCC CCAGCTACTT 
TACCAGGCCA TAGACTCCTA CAAGGAGGAC ATGGGCCCCG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTACAG TACGCCCTGA AGGCCCGCCC GCTGAGATGC 
TATATCCCCA AAAATCCATA CCAGTACCAG GTGTGGTACG TTGTCACCTC CTCCTACTTC 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCAG AGGAGATGAA CCACATCTCA GATATCCTCA ATGTGGCCTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAACTCATG GCATTCAAGG CCAGGGGCTA TTTTGGAGAC 
CCGTGGAACG TGTTCGACTT CTTGATTGTC ATCGGCAGCA TCATTGACGT CATCCTCAGT 
GAAATTGATA CTTTCCTGGC CTCCAGCGGA GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGACGAGAG CGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGGGCA GAGGGAGTCC GCACTCTCCT GTGGACGTTC 
ATCAAGTCCT TTCAGGCCCT GCCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCCGTCA TCGGCATGCA GATGTTTGGG AAGATCGCCA TGGTGGACGG GACCCAGATA 
AACCGGAACA ACAACTTCCA GACCTTCCCA CAAGCTGTGT TACTGCTCTT CAGGTGTGCG 
ACAGGGGAGG CGTGGCAGGA GATCTTGCTG GCCTGCAGCT ACGGCAAGTT GTGCGACCCC 
GAGTCAGACT ACGCCCCGGG CGAGGAGTAC ACGTGTGGCA CCAACTTCGC CTACTACTAT 
TTCATCAGCT TCTACATGCT GTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCTGTCATC 
ATGGACAACT TTGACTACCT CACGCGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATTTGGGC AGAGTATGAC CCAGAAGCCA AGGGCAGAAT CAAACACCTG 
GATGTGGTGA CGCTGCTGAG ACGCATCCAG CCCCCCCTGG GCTTTGGCAA GTTCTGCCCA 
CACCGGGTGG CCTGCAAGCG GCTGGTGGGC ATGAACATGC CCTTGAACAG CGACGGCACC 
GTCACCTTCA ACGCCACGCT CTTCGCGCTG GTCCGCACGG CCCTCAAGAT CAAGACGGAA 
GGTAACTTTG AGCAGGCCAA CGAGGAGCTG AGGGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCCTGGA CCAGGTCATT CCTCCAATAG GAGATGATGA GGTGACTGTA 
GGGAAGTTCT ACGCCACGTT TCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAAGAATATT ATGGGTATCG GCCCAAGAAG GACACCATAC AGATCCAGGC TGGACTGCGG 
ACCATTGAGG AAGAGGCAGC CCCAGAGATC CGCCGTACCA TCTCAGGAGA CCTGACAGCT 
GAAGAGGAGC TGGAGAGAGC CATGGTGGAG GCTGCGATGG AGGAAGGGAT ATTCCGGAGG 
ACCGGAGGCC TCTTTGGCCA GGTGGACAAC TTCCTGGAAA GGACCAACTC CCTGCCCCCT 
GTCATGGCCA ACCAGAGACC CCTCCAGTTT GCTGAGATAG AGATGGAAGA GCTGGAATCA 
CCCGTCTTCT TGGAGGATTT CCCTCAAGAT CCTAGAACCA ACCCCCTTGC TCGTGCCAAT 
ACCAACAATG CCAATGCCAA CGTTGCCCAT GACAACAGTG ACCATAGCAA CAGCCAGGTG 
TTTTCCAGCA TCCGCTACGA GAGGGAGTTC CCTGGAGAGC CAGAGCCGCC TGCTGCCAGA 
GGAGGAGCCT TGGGCCAACC CCGGAGGGTC CTGGGACCCC ACGGCAGGCC CTGTGTGGAA 
AAGCTGAAAG GACAGAGGAC CCAGAGAGAA ATGCCCAGAC CCCTGGCGTC TCCCGCCCCC 
TGCCAGTGTG CCGGGCCGGA GCGTCCCCTG CCCGAGGAGC GGAGTTGCCC CCCGCCGGGG 
TCTCTGCCTC AGGGAGCGCC CCCCAGCAGG AGGCCCACGG AGAGTGGCTG CAGGTGCCCG 
ACCCCAGCTA CGGCCCGGCT GGTTCGAGAG GCTCTGGTCC GAGGAGGCCT GGACACCTTG 
GCGGCCGATG CCAGCTTCGT CATGGCAACG GGCCAGGCCC TGGCAGATGC CTGCCGGATG 
GAACCCGAGG ACGTGGAGGT GGCGGCAACA GAGCTGCTCA AGAAGCAAGA GTCCCCAGAG 
GGCCGGGCCA GTGCGCTGGG ACGCCGGAGC CGCGGGTCCT CGCTGGGCAG CCTTGACCTG 
CATCTGGGCT CCCGGGAGAC CCTGGTGCCC CCCAGGCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe08962
Clone ID Related Accession (Same CDS sequence) XM_843592.5 , XM_843592.6
Accession Version XM_843592.6 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5622bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_843592.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
ATGGAGCCGT CCTCGCCCCA GGACGAGGGC CTGAGGAAGA AACAGCCCAA GAAGCCGGTT 
CCGGAGATCC TGCCGAGGCC GCCCCGGGCC CTGTTCTGCT TGACCCTGCA GAACCCCCTG 
AGGAAGGCCT GCATCAACAT TGTGGAATGG AAACCCTTCG AGACAATCAT CTTGCTCACC 
ATCTTTGCCA ACTGCGTGGC TCTGGCTGTG TACCTGCCCA TGCCGGAAGA CGACAACAAC 
ACTCTCAACC TTGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTTTT CTCCATCGAA 
GCGGCCATGA AGATCATCGC CTACGGCTTC CTCTTCCACC AGGACGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGACTT CATCATTGTC TTCCTGGGGG TTTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CAACACAGCC CCGATGAGCA GCAAAGGGGC CGGCCTGGAT 
GTCAAGGCCC TGAGAGCCTT CCGTGTGCTC AGACCCCTCC GGCTGGTGTC AGGGGTGCCG 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCCCTGTT CCACATCGCC 
CTGCTCGTCC TCTTTATGGT CATCATCTAC GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGG ACAGATATCG TGGCCACGGT GGAGAACGAG 
AAGCCCTCAC CCTGTGCCAG GACGGGCTCG GGGCGCCCAT GCACCATCAA TGGCAGCGAG 
TGTCGGGGCG GCTGGCCGGG GCCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATCACC ATGGAGGGGT GGACCGATGT CCTCTACTGG 
GTCAACGATG CCATCGGGAA CGAGTGGCCC TGGATCTATT TTGTCACCCT CATTCTGCTG 
GGATCCTTCT TCATTCTCAA CCTGGTGCTG GGTGTCCTGA GCGGGGAATT CACTAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GCGGCTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTCGAGGAC 
TTGAGAGAAG GGAAGCTGTC TTTGGATGAA GGAGGCTCCG ACACAGAGAG CCTGTACGAG 
ATCGAGGGCC TGAACAAAAT CATCCAGTTC GTCCGCCACT GGAGGCAGTG GAACCGGATC 
TTTCGCTGGA AGTGCCATGA CGTGATCAAG TCCAGGGTCT TCTACTGGCT GGTGATCCTG 
GTCGTCGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC CCTGTGGCTG 
ACCCACTTGC AAGACGTGGC CAACCGGGTG CTGCTGGCGC TCTTCACCGT CGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTCG TGGAATCGGG CGCCATGAGC 
CCCCTGGGCA TCTCCGTGCT GCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAG 
TACTGGACGT CCCTGAGCAA CCTGGTGGCG TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC ATCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTATGACTT CGAGGACACC GAGGTGCGGC GCAGCAACTT TGACAACTTC 
CCGCAGGCCC TCATCAGCGT CTTCCAGGTC CTGACGGGCG AGGACTGGAA CTCCATGATG 
TACAGCGGGA TCATGGCCTA CGGCGGCCCG TCCTACCCGG GGGTGCTCGT GTGCATTTAT 
TTCATCATTC TGTTCGTCTG TGGCAACTAT ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCGGAGGC AGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCGGAGGAG 
AGAAAGCGCA GGAAGATGTC CAAGGGTCTC CCAGATAAGT CAGAGGAGGA GAAGTCCGTG 
ATGGCCAAGA AGCTAGAGCA GAAACCCAAG GGTGAGGGCA TCCCTACCAC CGCCAAGCTG 
AAGATCGATG AGTTTGAATC GAATGTCAAC GAAGTGAAGG ATCCTTACCC CTCGGCTGAC 
TTCCCAGGCG ATGATGAGGA AGACGAGCCT GAGATCCCAG TGAGCCCCCG ACCACGGCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCA GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGTGTC CTGTGCCACC GCATTGTCAA CGCTACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CCGCCCTGGC CGCTGAGGAC 
CCCATCCGGG CCGAGTCTGT GAGGAATCAG ATCCTTGGGT ATTTTGACAT CGCTTTCACC 
TCTGTTTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGCGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGCACCATC TCCGTGGTGA AAATCCTGAG GGTGCTCAGG 
GTGCTCCGAC CCCTGCGGGC CATCAACAGA GCCAAGGGGC TGAAGCACGT GGTGCAGTGC 
GTGTTCGTGG CCATCCGCAC GATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTA 
TCCAAGATGA CAGAGCAGGA GTGCAGGGGC TATTACTACG TGTACAAGGA CGGGGACCCC 
ACGCAGATGG AGCTGCGTCC CCGCCAGTGG ATACACAGCG ACTTCCACTT TGACAACGTG 
CTCTCCGCCA TGATGTCACT CTTCACAGTG TCCACCTTTG AGGGATGGCC CCAGCTACTT 
TACCAGGCCA TAGACTCCTA CAAGGAGGAC ATGGGCCCCG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTACAG TACGCCCTGA AGGCCCGCCC GCTGAGATGC 
TATATCCCCA AAAATCCATA CCAGTACCAG GTGTGGTACG TTGTCACCTC CTCCTACTTC 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCAG AGGAGATGAA CCACATCTCA GATATCCTCA ATGTGGCCTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAACTCATG GCATTCAAGG CCAGGGGCTA TTTTGGAGAC 
CCGTGGAACG TGTTCGACTT CTTGATTGTC ATCGGCAGCA TCATTGACGT CATCCTCAGT 
GAAATTGATA CTTTCCTGGC CTCCAGCGGA GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGACGAGAG CGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGGGCA GAGGGAGTCC GCACTCTCCT GTGGACGTTC 
ATCAAGTCCT TTCAGGCCCT GCCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCCGTCA TCGGCATGCA GATGTTTGGG AAGATCGCCA TGGTGGACGG GACCCAGATA 
AACCGGAACA ACAACTTCCA GACCTTCCCA CAAGCTGTGT TACTGCTCTT CAGGTGTGCG 
ACAGGGGAGG CGTGGCAGGA GATCTTGCTG GCCTGCAGCT ACGGCAAGTT GTGCGACCCC 
GAGTCAGACT ACGCCCCGGG CGAGGAGTAC ACGTGTGGCA CCAACTTCGC CTACTACTAT 
TTCATCAGCT TCTACATGCT GTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCTGTCATC 
ATGGACAACT TTGACTACCT CACGCGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATTTGGGC AGAGTATGAC CCAGAAGCCA AGGGCAGAAT CAAACACCTG 
GATGTGGTGA CGCTGCTGAG ACGCATCCAG CCCCCCCTGG GCTTTGGCAA GTTCTGCCCA 
CACCGGGTGG CCTGCAAGCG GCTGGTGGGC ATGAACATGC CCTTGAACAG CGACGGCACC 
GTCACCTTCA ACGCCACGCT CTTCGCGCTG GTCCGCACGG CCCTCAAGAT CAAGACGGAA 
GGTAACTTTG AGCAGGCCAA CGAGGAGCTG AGGGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCCTGGA CCAGGTCATT CCTCCAATAG GAGATGATGA GGTGACTGTA 
GGGAAGTTCT ACGCCACGTT TCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAAGAATATT ATGGGTATCG GCCCAAGAAG GACACCATAC AGATCCAGGC TGGACTGCGG 
ACCATTGAGG AAGAGGCAGC CCCAGAGATC CGCCGTACCA TCTCAGGAGA CCTGACAGCT 
GAAGAGGAGC TGGAGAGAGC CATGGTGGAG GCTGCGATGG AGGAAGGGAT ATTCCGGAGG 
ACCGGAGGCC TCTTTGGCCA GGTGGACAAC TTCCTGGAAA GGACCAACTC CCTGCCCCCT 
GTCATGGCCA ACCAGAGACC CCTCCAGTTT GCTGAGATAG AGATGGAAGA GCTGGAATCA 
CCCGTCTTCT TGGAGGATTT CCCTCAAGAT CCTAGAACCA ACCCCCTTGC TCGTGCCAAT 
ACCAACAATG CCAATGCCAA CGTTGCCCAT GACAACAGTG ACCATAGCAA CAGCCAGGTG 
TTTTCCAGCA TCCGCTACGA GAGGGAGTTC CCTGGAGAGC CAGAGCCGCC TGCTGCCAGA 
GGAGGAGCCT TGGGCCAACC CCGGAGGGTC CTGGGACCCC ACGGCAGGCC CTGTGTGGAA 
AAGCTGAAAG GACAGAGGAC CCAGAGAGAA ATGCCCAGAC CCCTGGCGTC TCCCGCCCCC 
TGCCAGTGTG CCGGGCCGGA GCGTCCCCTG CCCGAGGAGC GGAGTTGCCC CCCGCCGGGG 
TCTCTGCCTC AGGGAGCGCC CCCCAGCAGG AGGCCCACGG AGAGTGGCTG CAGGTGCCCG 
ACCCCAGCTA CGGCCCGGCT GGTTCGAGAG GCTCTGGTCC GAGGAGGCCT GGACACCTTG 
GCGGCCGATG CCAGCTTCGT CATGGCAACG GGCCAGGCCC TGGCAGATGC CTGCCGGATG 
GAACCCGAGG ACGTGGAGGT GGCGGCAACA GAGCTGCTCA AGAAGCAAGA GTCCCCAGAG 
GGCCGGGCCA GTGCGCTGGG ACGCCGGAGC CGCGGGTCCT CGCTGGGCAG CCTTGACCTG 
CATCTGGGCT CCCGGGAGAC CCTGGTGCCC CCCAGGCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_848685.3
CDS114..5735
Translation

Target ORF information:

RefSeq Version XM_843592.6
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_843592.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
ATGGAGCCGT CCTCGCCCCA GGACGAGGGC CTGAGGAAGA AACAGCCCAA GAAGCCGGTT 
CCGGAGATCC TGCCGAGGCC GCCCCGGGCC CTGTTCTGCT TGACCCTGCA GAACCCCCTG 
AGGAAGGCCT GCATCAACAT TGTGGAATGG AAACCCTTCG AGACAATCAT CTTGCTCACC 
ATCTTTGCCA ACTGCGTGGC TCTGGCTGTG TACCTGCCCA TGCCGGAAGA CGACAACAAC 
ACTCTCAACC TTGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTTTT CTCCATCGAA 
GCGGCCATGA AGATCATCGC CTACGGCTTC CTCTTCCACC AGGACGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGACTT CATCATTGTC TTCCTGGGGG TTTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CAACACAGCC CCGATGAGCA GCAAAGGGGC CGGCCTGGAT 
GTCAAGGCCC TGAGAGCCTT CCGTGTGCTC AGACCCCTCC GGCTGGTGTC AGGGGTGCCG 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCCCTGTT CCACATCGCC 
CTGCTCGTCC TCTTTATGGT CATCATCTAC GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGG ACAGATATCG TGGCCACGGT GGAGAACGAG 
AAGCCCTCAC CCTGTGCCAG GACGGGCTCG GGGCGCCCAT GCACCATCAA TGGCAGCGAG 
TGTCGGGGCG GCTGGCCGGG GCCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATCACC ATGGAGGGGT GGACCGATGT CCTCTACTGG 
GTCAACGATG CCATCGGGAA CGAGTGGCCC TGGATCTATT TTGTCACCCT CATTCTGCTG 
GGATCCTTCT TCATTCTCAA CCTGGTGCTG GGTGTCCTGA GCGGGGAATT CACTAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GCGGCTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTCGAGGAC 
TTGAGAGAAG GGAAGCTGTC TTTGGATGAA GGAGGCTCCG ACACAGAGAG CCTGTACGAG 
ATCGAGGGCC TGAACAAAAT CATCCAGTTC GTCCGCCACT GGAGGCAGTG GAACCGGATC 
TTTCGCTGGA AGTGCCATGA CGTGATCAAG TCCAGGGTCT TCTACTGGCT GGTGATCCTG 
GTCGTCGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC CCTGTGGCTG 
ACCCACTTGC AAGACGTGGC CAACCGGGTG CTGCTGGCGC TCTTCACCGT CGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTCG TGGAATCGGG CGCCATGAGC 
CCCCTGGGCA TCTCCGTGCT GCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAG 
TACTGGACGT CCCTGAGCAA CCTGGTGGCG TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC ATCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTATGACTT CGAGGACACC GAGGTGCGGC GCAGCAACTT TGACAACTTC 
CCGCAGGCCC TCATCAGCGT CTTCCAGGTC CTGACGGGCG AGGACTGGAA CTCCATGATG 
TACAGCGGGA TCATGGCCTA CGGCGGCCCG TCCTACCCGG GGGTGCTCGT GTGCATTTAT 
TTCATCATTC TGTTCGTCTG TGGCAACTAT ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCGGAGGC AGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCGGAGGAG 
AGAAAGCGCA GGAAGATGTC CAAGGGTCTC CCAGATAAGT CAGAGGAGGA GAAGTCCGTG 
ATGGCCAAGA AGCTAGAGCA GAAACCCAAG GGTGAGGGCA TCCCTACCAC CGCCAAGCTG 
AAGATCGATG AGTTTGAATC GAATGTCAAC GAAGTGAAGG ATCCTTACCC CTCGGCTGAC 
TTCCCAGGCG ATGATGAGGA AGACGAGCCT GAGATCCCAG TGAGCCCCCG ACCACGGCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCA GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGTGTC CTGTGCCACC GCATTGTCAA CGCTACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CCGCCCTGGC CGCTGAGGAC 
CCCATCCGGG CCGAGTCTGT GAGGAATCAG ATCCTTGGGT ATTTTGACAT CGCTTTCACC 
TCTGTTTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGCGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGCACCATC TCCGTGGTGA AAATCCTGAG GGTGCTCAGG 
GTGCTCCGAC CCCTGCGGGC CATCAACAGA GCCAAGGGGC TGAAGCACGT GGTGCAGTGC 
GTGTTCGTGG CCATCCGCAC GATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTA 
TCCAAGATGA CAGAGCAGGA GTGCAGGGGC TATTACTACG TGTACAAGGA CGGGGACCCC 
ACGCAGATGG AGCTGCGTCC CCGCCAGTGG ATACACAGCG ACTTCCACTT TGACAACGTG 
CTCTCCGCCA TGATGTCACT CTTCACAGTG TCCACCTTTG AGGGATGGCC CCAGCTACTT 
TACCAGGCCA TAGACTCCTA CAAGGAGGAC ATGGGCCCCG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTACAG TACGCCCTGA AGGCCCGCCC GCTGAGATGC 
TATATCCCCA AAAATCCATA CCAGTACCAG GTGTGGTACG TTGTCACCTC CTCCTACTTC 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCAG AGGAGATGAA CCACATCTCA GATATCCTCA ATGTGGCCTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAACTCATG GCATTCAAGG CCAGGGGCTA TTTTGGAGAC 
CCGTGGAACG TGTTCGACTT CTTGATTGTC ATCGGCAGCA TCATTGACGT CATCCTCAGT 
GAAATTGATA CTTTCCTGGC CTCCAGCGGA GGACTGTATT GCCTGGGTGG AGGCTGCGGG 
AACGTTGACC CAGACGAGAG CGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGGGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGGGCA GAGGGAGTCC GCACTCTCCT GTGGACGTTC 
ATCAAGTCCT TTCAGGCCCT GCCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCCGTCA TCGGCATGCA GATGTTTGGG AAGATCGCCA TGGTGGACGG GACCCAGATA 
AACCGGAACA ACAACTTCCA GACCTTCCCA CAAGCTGTGT TACTGCTCTT CAGGTGTGCG 
ACAGGGGAGG CGTGGCAGGA GATCTTGCTG GCCTGCAGCT ACGGCAAGTT GTGCGACCCC 
GAGTCAGACT ACGCCCCGGG CGAGGAGTAC ACGTGTGGCA CCAACTTCGC CTACTACTAT 
TTCATCAGCT TCTACATGCT GTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCTGTCATC 
ATGGACAACT TTGACTACCT CACGCGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATTTGGGC AGAGTATGAC CCAGAAGCCA AGGGCAGAAT CAAACACCTG 
GATGTGGTGA CGCTGCTGAG ACGCATCCAG CCCCCCCTGG GCTTTGGCAA GTTCTGCCCA 
CACCGGGTGG CCTGCAAGCG GCTGGTGGGC ATGAACATGC CCTTGAACAG CGACGGCACC 
GTCACCTTCA ACGCCACGCT CTTCGCGCTG GTCCGCACGG CCCTCAAGAT CAAGACGGAA 
GGTAACTTTG AGCAGGCCAA CGAGGAGCTG AGGGCCATCA TCAAGAAGAT CTGGAAGAGA 
ACCAGCATGA AGCTCCTGGA CCAGGTCATT CCTCCAATAG GAGATGATGA GGTGACTGTA 
GGGAAGTTCT ACGCCACGTT TCTCATCCAG GAGCACTTCC GGAAGTTCAT GAAGCGCCAG 
GAAGAATATT ATGGGTATCG GCCCAAGAAG GACACCATAC AGATCCAGGC TGGACTGCGG 
ACCATTGAGG AAGAGGCAGC CCCAGAGATC CGCCGTACCA TCTCAGGAGA CCTGACAGCT 
GAAGAGGAGC TGGAGAGAGC CATGGTGGAG GCTGCGATGG AGGAAGGGAT ATTCCGGAGG 
ACCGGAGGCC TCTTTGGCCA GGTGGACAAC TTCCTGGAAA GGACCAACTC CCTGCCCCCT 
GTCATGGCCA ACCAGAGACC CCTCCAGTTT GCTGAGATAG AGATGGAAGA GCTGGAATCA 
CCCGTCTTCT TGGAGGATTT CCCTCAAGAT CCTAGAACCA ACCCCCTTGC TCGTGCCAAT 
ACCAACAATG CCAATGCCAA CGTTGCCCAT GACAACAGTG ACCATAGCAA CAGCCAGGTG 
TTTTCCAGCA TCCGCTACGA GAGGGAGTTC CCTGGAGAGC CAGAGCCGCC TGCTGCCAGA 
GGAGGAGCCT TGGGCCAACC CCGGAGGGTC CTGGGACCCC ACGGCAGGCC CTGTGTGGAA 
AAGCTGAAAG GACAGAGGAC CCAGAGAGAA ATGCCCAGAC CCCTGGCGTC TCCCGCCCCC 
TGCCAGTGTG CCGGGCCGGA GCGTCCCCTG CCCGAGGAGC GGAGTTGCCC CCCGCCGGGG 
TCTCTGCCTC AGGGAGCGCC CCCCAGCAGG AGGCCCACGG AGAGTGGCTG CAGGTGCCCG 
ACCCCAGCTA CGGCCCGGCT GGTTCGAGAG GCTCTGGTCC GAGGAGGCCT GGACACCTTG 
GCGGCCGATG CCAGCTTCGT CATGGCAACG GGCCAGGCCC TGGCAGATGC CTGCCGGATG 
GAACCCGAGG ACGTGGAGGT GGCGGCAACA GAGCTGCTCA AGAAGCAAGA GTCCCCAGAG 
GGCCGGGCCA GTGCGCTGGG ACGCCGGAGC CGCGGGTCCT CGCTGGGCAG CCTTGACCTG 
CATCTGGGCT CCCGGGAGAC CCTGGTGCCC CCCAGGCAGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe08963
Clone ID Related Accession (Same CDS sequence) XM_005622266.2 , XM_005622266.3
Accession Version XM_005622266.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5565bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-09-16
Organism Canis lupus familiaris(Dog)
Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGT CCTCGCCCCA GGACGAGGGC CTGAGGAAGA AACAGCCCAA GAAGCCGGTT 
CCGGAGATCC TGCCGAGGCC GCCCCGGGCC CTGTTCTGCT TGACCCTGCA GAACCCCCTG 
AGGAAGGCCT GCATCAACAT TGTGGAATGG AAACCCTTCG AGACAATCAT CTTGCTCACC 
ATCTTTGCCA ACTGCGTGGC TCTGGCTGTG TACCTGCCCA TGCCGGAAGA CGACAACAAC 
ACTCTCAACC TTGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTTTT CTCCATCGAA 
GCGGCCATGA AGATCATCGC CTACGGCTTC CTCTTCCACC AGGACGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGACTT CATCATTGTC TTCCTGGGGG TTTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CAACACAGCC CCGATGAGCA GCAAAGGGGC CGGCCTGGAT 
GTCAAGGCCC TGAGAGCCTT CCGTGTGCTC AGACCCCTCC GGCTGGTGTC AGGGGTGCCG 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCCCTGTT CCACATCGCC 
CTGCTCGTCC TCTTTATGGT CATCATCTAC GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGG ACAGATATCG TGGCCACGGT GGAGAACGAG 
AAGCCCTCAC CCTGTGCCAG GACGGGCTCG GGGCGCCCAT GCACCATCAA TGGCAGCGAG 
TGTCGGGGCG GCTGGCCGGG GCCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATCACC ATGGAGGGGT GGACCGATGT CCTCTACTGG 
GTCAACGATG CCATCGGGAA CGAGTGGCCC TGGATCTATT TTGTCACCCT CATTCTGCTG 
GGATCCTTCT TCATTCTCAA CCTGGTGCTG GGTGTCCTGA GCGGGGAATT CACTAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GCGGCTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTCGAGGAC 
TTGAGAGAAG GGAAGCTGTC TTTGGATGAA GGAGGCTCCG ACACAGAGAG CCTGTACGAG 
ATCGAGGGCC TGAACAAAAT CATCCAGTTC GTCCGCCACT GGAGGCAGTG GAACCGGATC 
TTTCGCTGGA AGTGCCATGA CGTGATCAAG TCCAGGGTCT TCTACTGGCT GGTGATCCTG 
GTCGTCGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC CCTGTGGCTG 
ACCCACTTGC AAGACGTGGC CAACCGGGTG CTGCTGGCGC TCTTCACCGT CGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTCG TGGAATCGGG CGCCATGAGC 
CCCCTGGGCA TCTCCGTGCT GCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAG 
TACTGGACGT CCCTGAGCAA CCTGGTGGCG TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC ATCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTATGACTT CGAGGACACC GAGGTGCGGC GCAGCAACTT TGACAACTTC 
CCGCAGGCCC TCATCAGCGT CTTCCAGGTC CTGACGGGCG AGGACTGGAA CTCCATGATG 
TACAGCGGGA TCATGGCCTA CGGCGGCCCG TCCTACCCGG GGGTGCTCGT GTGCATTTAT 
TTCATCATTC TGTTCGTCTG TGGCAACTAT ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCGGAGGC AGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCGGAGGAG 
AGAAAGCGCA GGAAGATGTC CAAGGGTCTC CCAGATAAGT CAGAGGAGGA GAAGTCCGTG 
ATGGCCAAGA AGCTAGAGCA GAAACCCAAG GGTGAGGGCA TCCCTACCAC CGCCAAGCTG 
AAGATCGATG AGTTTGAATC GAATGTCAAC GAAGTGAAGG ATCCTTACCC CTCGGCTGAC 
TTCCCAGGCG ATGATGAGGA AGACGAGCCT GAGATCCCAG TGAGCCCCCG ACCACGGCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCA GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGTGTC CTGTGCCACC GCATTGTCAA CGCTACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CCGCCCTGGC CGCTGAGGAC 
CCCATCCGGG CCGAGTCTGT GAGGAATCAG ATCCTTGGGT ATTTTGACAT CGCTTTCACC 
TCTGTTTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGCGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGCACCATC TCCGTGGTGA AAATCCTGAG GGTGCTCAGG 
GTGCTCCGAC CCCTGCGGGC CATCAACAGA GCCAAGGGGC TGAAGCACGT GGTGCAGTGC 
GTGTTCGTGG CCATCCGCAC GATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTA 
TCCAAGATGA CAGAGCAGGA GTGCAGGGGC TATTACTACG TGTACAAGGA CGGGGACCCC 
ACGCAGATGG AGCTGCGTCC CCGCCAGTGG ATACACAGCG ACTTCCACTT TGACAACGTG 
CTCTCCGCCA TGATGTCACT CTTCACAGTG TCCACCTTTG AGGGATGGCC CCAGCTACTT 
TACCAGGCCA TAGACTCCTA CAAGGAGGAC ATGGGCCCCG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTACAG TACGCCCTGA AGGCCCGCCC GCTGAGATGC 
TATATCCCCA AAAATCCATA CCAGTACCAG GTGTGGTACG TTGTCACCTC CTCCTACTTC 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCAG AGGAGATGAA CCACATCTCA GATATCCTCA ATGTGGCCTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAACTCATG GCATTCAAGG CCAGGGGCTA TTTTGGAGAC 
CCGTGGAACG TGTTCGACTT CTTGATTGTC ATCGGCAGCA TCATTGACGT CATCCTCAGT 
GAAATTGATG ACCCAGACGA GAGCGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGG 
GTCATGAGGC TGATCAAGCT GCTGAGCCGG GCAGAGGGAG TCCGCACTCT CCTGTGGACG 
TTCATCAAGT CCTTTCAGGC CCTGCCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC 
ATCTACGCCG TCATCGGCAT GCAGATGTTT GGGAAGATCG CCATGGTGGA CGGGACCCAG 
ATAAACCGGA ACAACAACTT CCAGACCTTC CCACAAGCTG TGTTACTGCT CTTCAGGTGT 
GCGACAGGGG AGGCGTGGCA GGAGATCTTG CTGGCCTGCA GCTACGGCAA GTTGTGCGAC 
CCCGAGTCAG ACTACGCCCC GGGCGAGGAG TACACGTGTG GCACCAACTT CGCCTACTAC 
TATTTCATCA GCTTCTACAT GCTGTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCTGTC 
ATCATGGACA ACTTTGACTA CCTCACGCGG GACTGGTCCA TCCTGGGCCC TCATCACCTG 
GATGAGTTCA AGGCCATTTG GGCAGAGTAT GACCCAGAAG CCAAGGGCAG AATCAAACAC 
CTGGATGTGG TGACGCTGCT GAGACGCATC CAGCCCCCCC TGGGCTTTGG CAAGTTCTGC 
CCACACCGGG TGGCCTGCAA GCGGCTGGTG GGCATGAACA TGCCCTTGAA CAGCGACGGC 
ACCGTCACCT TCAACGCCAC GCTCTTCGCG CTGGTCCGCA CGGCCCTCAA GATCAAGACG 
GAAGGTAACT TTGAGCAGGC CAACGAGGAG CTGAGGGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCCT GGACCAGGTC ATTCCTCCAA TAGGAGATGA TGAGGTGACT 
GTAGGGAAGT TCTACGCCAC GTTTCTCATC CAGGAGCACT TCCGGAAGTT CATGAAGCGC 
CAGGAAGAAT ATTATGGGTA TCGGCCCAAG AAGGACACCA TACAGATCCA GGCTGGACTG 
CGGACCATTG AGGAAGAGGC AGCCCCAGAG ATCCGCCGTA CCATCTCAGG AGACCTGACA 
GCTGAAGAGG AGCTGGAGAG AGCCATGGTG GAGGCTGCGA TGGAGGAAGG GATATTCCGG 
AGGACCGGAG GCCTCTTTGG CCAGGTGGAC AACTTCCTGG AAAGGACCAA CTCCCTGCCC 
CCTGTCATGG CCAACCAGAG ACCCCTCCAG TTTGCTGAGA TAGAGATGGA AGAGCTGGAA 
TCACCCGTCT TCTTGGAGGA TTTCCCTCAA GATCCTAGAA CCAACCCCCT TGCTCGTGCC 
AATACCAACA ATGCCAATGC CAACGTTGCC CATGACAACA GTGACCATAG CAACAGCCAG 
GTGTTTTCCA GCATCCGCTA CGAGAGGGAG TTCCCTGGAG AGCCAGAGCC GCCTGCTGCC 
AGAGGAGGAG CCTTGGGCCA ACCCCGGAGG GTCCTGGGAC CCCACGGCAG GCCCTGTGTG 
GAAAAGCTGA AAGGACAGAG GACCCAGAGA GAAATGCCCA GACCCCTGGC GTCTCCCGCC 
CCCTGCCAGT GTGCCGGGCC GGAGCGTCCC CTGCCCGAGG AGCGGAGTTG CCCCCCGCCG 
GGGTCTCTGC CTCAGGGAGC GCCCCCCAGC AGGAGGCCCA CGGAGAGTGG CTGCAGGTGC 
CCGACCCCAG CTACGGCCCG GCTGGTTCGA GAGGCTCTGG TCCGAGGAGG CCTGGACACC 
TTGGCGGCCG ATGCCAGCTT CGTCATGGCA ACGGGCCAGG CCCTGGCAGA TGCCTGCCGG 
ATGGAACCCG AGGACGTGGA GGTGGCGGCA ACAGAGCTGC TCAAGAAGCA AGAGTCCCCA 
GAGGGCCGGG CCAGTGCGCT GGGACGCCGG AGCCGCGGGT CCTCGCTGGG CAGCCTTGAC 
CTGCATCTGG GCTCCCGGGA GACCCTGGTG CCCCCCAGGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005622323.1
CDS114..5678
Translation

Target ORF information:

RefSeq Version XM_005622266.2
Organism Canis lupus familiaris(Dog)
Definition Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005622266.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGT CCTCGCCCCA GGACGAGGGC CTGAGGAAGA AACAGCCCAA GAAGCCGGTT 
CCGGAGATCC TGCCGAGGCC GCCCCGGGCC CTGTTCTGCT TGACCCTGCA GAACCCCCTG 
AGGAAGGCCT GCATCAACAT TGTGGAATGG AAACCCTTCG AGACAATCAT CTTGCTCACC 
ATCTTTGCCA ACTGCGTGGC TCTGGCTGTG TACCTGCCCA TGCCGGAAGA CGACAACAAC 
ACTCTCAACC TTGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTTTT CTCCATCGAA 
GCGGCCATGA AGATCATCGC CTACGGCTTC CTCTTCCACC AGGACGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGACTT CATCATTGTC TTCCTGGGGG TTTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CAACACAGCC CCGATGAGCA GCAAAGGGGC CGGCCTGGAT 
GTCAAGGCCC TGAGAGCCTT CCGTGTGCTC AGACCCCTCC GGCTGGTGTC AGGGGTGCCG 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCCCTGTT CCACATCGCC 
CTGCTCGTCC TCTTTATGGT CATCATCTAC GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGG ACAGATATCG TGGCCACGGT GGAGAACGAG 
AAGCCCTCAC CCTGTGCCAG GACGGGCTCG GGGCGCCCAT GCACCATCAA TGGCAGCGAG 
TGTCGGGGCG GCTGGCCGGG GCCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATCACC ATGGAGGGGT GGACCGATGT CCTCTACTGG 
GTCAACGATG CCATCGGGAA CGAGTGGCCC TGGATCTATT TTGTCACCCT CATTCTGCTG 
GGATCCTTCT TCATTCTCAA CCTGGTGCTG GGTGTCCTGA GCGGGGAATT CACTAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GCGGCTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTCGAGGAC 
TTGAGAGAAG GGAAGCTGTC TTTGGATGAA GGAGGCTCCG ACACAGAGAG CCTGTACGAG 
ATCGAGGGCC TGAACAAAAT CATCCAGTTC GTCCGCCACT GGAGGCAGTG GAACCGGATC 
TTTCGCTGGA AGTGCCATGA CGTGATCAAG TCCAGGGTCT TCTACTGGCT GGTGATCCTG 
GTCGTCGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC CCTGTGGCTG 
ACCCACTTGC AAGACGTGGC CAACCGGGTG CTGCTGGCGC TCTTCACCGT CGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTCG TGGAATCGGG CGCCATGAGC 
CCCCTGGGCA TCTCCGTGCT GCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAG 
TACTGGACGT CCCTGAGCAA CCTGGTGGCG TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC ATCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTATGACTT CGAGGACACC GAGGTGCGGC GCAGCAACTT TGACAACTTC 
CCGCAGGCCC TCATCAGCGT CTTCCAGGTC CTGACGGGCG AGGACTGGAA CTCCATGATG 
TACAGCGGGA TCATGGCCTA CGGCGGCCCG TCCTACCCGG GGGTGCTCGT GTGCATTTAT 
TTCATCATTC TGTTCGTCTG TGGCAACTAT ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCGGAGGC AGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCGGAGGAG 
AGAAAGCGCA GGAAGATGTC CAAGGGTCTC CCAGATAAGT CAGAGGAGGA GAAGTCCGTG 
ATGGCCAAGA AGCTAGAGCA GAAACCCAAG GGTGAGGGCA TCCCTACCAC CGCCAAGCTG 
AAGATCGATG AGTTTGAATC GAATGTCAAC GAAGTGAAGG ATCCTTACCC CTCGGCTGAC 
TTCCCAGGCG ATGATGAGGA AGACGAGCCT GAGATCCCAG TGAGCCCCCG ACCACGGCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCA GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGTGTC CTGTGCCACC GCATTGTCAA CGCTACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CCGCCCTGGC CGCTGAGGAC 
CCCATCCGGG CCGAGTCTGT GAGGAATCAG ATCCTTGGGT ATTTTGACAT CGCTTTCACC 
TCTGTTTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGCGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGCACCATC TCCGTGGTGA AAATCCTGAG GGTGCTCAGG 
GTGCTCCGAC CCCTGCGGGC CATCAACAGA GCCAAGGGGC TGAAGCACGT GGTGCAGTGC 
GTGTTCGTGG CCATCCGCAC GATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTA 
TCCAAGATGA CAGAGCAGGA GTGCAGGGGC TATTACTACG TGTACAAGGA CGGGGACCCC 
ACGCAGATGG AGCTGCGTCC CCGCCAGTGG ATACACAGCG ACTTCCACTT TGACAACGTG 
CTCTCCGCCA TGATGTCACT CTTCACAGTG TCCACCTTTG AGGGATGGCC CCAGCTACTT 
TACCAGGCCA TAGACTCCTA CAAGGAGGAC ATGGGCCCCG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTACAG TACGCCCTGA AGGCCCGCCC GCTGAGATGC 
TATATCCCCA AAAATCCATA CCAGTACCAG GTGTGGTACG TTGTCACCTC CTCCTACTTC 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCAG AGGAGATGAA CCACATCTCA GATATCCTCA ATGTGGCCTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAACTCATG GCATTCAAGG CCAGGGGCTA TTTTGGAGAC 
CCGTGGAACG TGTTCGACTT CTTGATTGTC ATCGGCAGCA TCATTGACGT CATCCTCAGT 
GAAATTGATG ACCCAGACGA GAGCGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGG 
GTCATGAGGC TGATCAAGCT GCTGAGCCGG GCAGAGGGAG TCCGCACTCT CCTGTGGACG 
TTCATCAAGT CCTTTCAGGC CCTGCCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC 
ATCTACGCCG TCATCGGCAT GCAGATGTTT GGGAAGATCG CCATGGTGGA CGGGACCCAG 
ATAAACCGGA ACAACAACTT CCAGACCTTC CCACAAGCTG TGTTACTGCT CTTCAGGTGT 
GCGACAGGGG AGGCGTGGCA GGAGATCTTG CTGGCCTGCA GCTACGGCAA GTTGTGCGAC 
CCCGAGTCAG ACTACGCCCC GGGCGAGGAG TACACGTGTG GCACCAACTT CGCCTACTAC 
TATTTCATCA GCTTCTACAT GCTGTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCTGTC 
ATCATGGACA ACTTTGACTA CCTCACGCGG GACTGGTCCA TCCTGGGCCC TCATCACCTG 
GATGAGTTCA AGGCCATTTG GGCAGAGTAT GACCCAGAAG CCAAGGGCAG AATCAAACAC 
CTGGATGTGG TGACGCTGCT GAGACGCATC CAGCCCCCCC TGGGCTTTGG CAAGTTCTGC 
CCACACCGGG TGGCCTGCAA GCGGCTGGTG GGCATGAACA TGCCCTTGAA CAGCGACGGC 
ACCGTCACCT TCAACGCCAC GCTCTTCGCG CTGGTCCGCA CGGCCCTCAA GATCAAGACG 
GAAGGTAACT TTGAGCAGGC CAACGAGGAG CTGAGGGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCCT GGACCAGGTC ATTCCTCCAA TAGGAGATGA TGAGGTGACT 
GTAGGGAAGT TCTACGCCAC GTTTCTCATC CAGGAGCACT TCCGGAAGTT CATGAAGCGC 
CAGGAAGAAT ATTATGGGTA TCGGCCCAAG AAGGACACCA TACAGATCCA GGCTGGACTG 
CGGACCATTG AGGAAGAGGC AGCCCCAGAG ATCCGCCGTA CCATCTCAGG AGACCTGACA 
GCTGAAGAGG AGCTGGAGAG AGCCATGGTG GAGGCTGCGA TGGAGGAAGG GATATTCCGG 
AGGACCGGAG GCCTCTTTGG CCAGGTGGAC AACTTCCTGG AAAGGACCAA CTCCCTGCCC 
CCTGTCATGG CCAACCAGAG ACCCCTCCAG TTTGCTGAGA TAGAGATGGA AGAGCTGGAA 
TCACCCGTCT TCTTGGAGGA TTTCCCTCAA GATCCTAGAA CCAACCCCCT TGCTCGTGCC 
AATACCAACA ATGCCAATGC CAACGTTGCC CATGACAACA GTGACCATAG CAACAGCCAG 
GTGTTTTCCA GCATCCGCTA CGAGAGGGAG TTCCCTGGAG AGCCAGAGCC GCCTGCTGCC 
AGAGGAGGAG CCTTGGGCCA ACCCCGGAGG GTCCTGGGAC CCCACGGCAG GCCCTGTGTG 
GAAAAGCTGA AAGGACAGAG GACCCAGAGA GAAATGCCCA GACCCCTGGC GTCTCCCGCC 
CCCTGCCAGT GTGCCGGGCC GGAGCGTCCC CTGCCCGAGG AGCGGAGTTG CCCCCCGCCG 
GGGTCTCTGC CTCAGGGAGC GCCCCCCAGC AGGAGGCCCA CGGAGAGTGG CTGCAGGTGC 
CCGACCCCAG CTACGGCCCG GCTGGTTCGA GAGGCTCTGG TCCGAGGAGG CCTGGACACC 
TTGGCGGCCG ATGCCAGCTT CGTCATGGCA ACGGGCCAGG CCCTGGCAGA TGCCTGCCGG 
ATGGAACCCG AGGACGTGGA GGTGGCGGCA ACAGAGCTGC TCAAGAAGCA AGAGTCCCCA 
GAGGGCCGGG CCAGTGCGCT GGGACGCCGG AGCCGCGGGT CCTCGCTGGG CAGCCTTGAC 
CTGCATCTGG GCTCCCGGGA GACCCTGGTG CCCCCCAGGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OCe08963
Clone ID Related Accession (Same CDS sequence) XM_005622266.2 , XM_005622266.3
Accession Version XM_005622266.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5565bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-09-04
Organism Canis lupus familiaris(dog)
Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005622266.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGT CCTCGCCCCA GGACGAGGGC CTGAGGAAGA AACAGCCCAA GAAGCCGGTT 
CCGGAGATCC TGCCGAGGCC GCCCCGGGCC CTGTTCTGCT TGACCCTGCA GAACCCCCTG 
AGGAAGGCCT GCATCAACAT TGTGGAATGG AAACCCTTCG AGACAATCAT CTTGCTCACC 
ATCTTTGCCA ACTGCGTGGC TCTGGCTGTG TACCTGCCCA TGCCGGAAGA CGACAACAAC 
ACTCTCAACC TTGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTTTT CTCCATCGAA 
GCGGCCATGA AGATCATCGC CTACGGCTTC CTCTTCCACC AGGACGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGACTT CATCATTGTC TTCCTGGGGG TTTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CAACACAGCC CCGATGAGCA GCAAAGGGGC CGGCCTGGAT 
GTCAAGGCCC TGAGAGCCTT CCGTGTGCTC AGACCCCTCC GGCTGGTGTC AGGGGTGCCG 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCCCTGTT CCACATCGCC 
CTGCTCGTCC TCTTTATGGT CATCATCTAC GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGG ACAGATATCG TGGCCACGGT GGAGAACGAG 
AAGCCCTCAC CCTGTGCCAG GACGGGCTCG GGGCGCCCAT GCACCATCAA TGGCAGCGAG 
TGTCGGGGCG GCTGGCCGGG GCCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATCACC ATGGAGGGGT GGACCGATGT CCTCTACTGG 
GTCAACGATG CCATCGGGAA CGAGTGGCCC TGGATCTATT TTGTCACCCT CATTCTGCTG 
GGATCCTTCT TCATTCTCAA CCTGGTGCTG GGTGTCCTGA GCGGGGAATT CACTAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GCGGCTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTCGAGGAC 
TTGAGAGAAG GGAAGCTGTC TTTGGATGAA GGAGGCTCCG ACACAGAGAG CCTGTACGAG 
ATCGAGGGCC TGAACAAAAT CATCCAGTTC GTCCGCCACT GGAGGCAGTG GAACCGGATC 
TTTCGCTGGA AGTGCCATGA CGTGATCAAG TCCAGGGTCT TCTACTGGCT GGTGATCCTG 
GTCGTCGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC CCTGTGGCTG 
ACCCACTTGC AAGACGTGGC CAACCGGGTG CTGCTGGCGC TCTTCACCGT CGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTCG TGGAATCGGG CGCCATGAGC 
CCCCTGGGCA TCTCCGTGCT GCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAG 
TACTGGACGT CCCTGAGCAA CCTGGTGGCG TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC ATCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTATGACTT CGAGGACACC GAGGTGCGGC GCAGCAACTT TGACAACTTC 
CCGCAGGCCC TCATCAGCGT CTTCCAGGTC CTGACGGGCG AGGACTGGAA CTCCATGATG 
TACAGCGGGA TCATGGCCTA CGGCGGCCCG TCCTACCCGG GGGTGCTCGT GTGCATTTAT 
TTCATCATTC TGTTCGTCTG TGGCAACTAT ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCGGAGGC AGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCGGAGGAG 
AGAAAGCGCA GGAAGATGTC CAAGGGTCTC CCAGATAAGT CAGAGGAGGA GAAGTCCGTG 
ATGGCCAAGA AGCTAGAGCA GAAACCCAAG GGTGAGGGCA TCCCTACCAC CGCCAAGCTG 
AAGATCGATG AGTTTGAATC GAATGTCAAC GAAGTGAAGG ATCCTTACCC CTCGGCTGAC 
TTCCCAGGCG ATGATGAGGA AGACGAGCCT GAGATCCCAG TGAGCCCCCG ACCACGGCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCA GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGTGTC CTGTGCCACC GCATTGTCAA CGCTACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CCGCCCTGGC CGCTGAGGAC 
CCCATCCGGG CCGAGTCTGT GAGGAATCAG ATCCTTGGGT ATTTTGACAT CGCTTTCACC 
TCTGTTTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGCGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGCACCATC TCCGTGGTGA AAATCCTGAG GGTGCTCAGG 
GTGCTCCGAC CCCTGCGGGC CATCAACAGA GCCAAGGGGC TGAAGCACGT GGTGCAGTGC 
GTGTTCGTGG CCATCCGCAC GATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTA 
TCCAAGATGA CAGAGCAGGA GTGCAGGGGC TATTACTACG TGTACAAGGA CGGGGACCCC 
ACGCAGATGG AGCTGCGTCC CCGCCAGTGG ATACACAGCG ACTTCCACTT TGACAACGTG 
CTCTCCGCCA TGATGTCACT CTTCACAGTG TCCACCTTTG AGGGATGGCC CCAGCTACTT 
TACCAGGCCA TAGACTCCTA CAAGGAGGAC ATGGGCCCCG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTACAG TACGCCCTGA AGGCCCGCCC GCTGAGATGC 
TATATCCCCA AAAATCCATA CCAGTACCAG GTGTGGTACG TTGTCACCTC CTCCTACTTC 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCAG AGGAGATGAA CCACATCTCA GATATCCTCA ATGTGGCCTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAACTCATG GCATTCAAGG CCAGGGGCTA TTTTGGAGAC 
CCGTGGAACG TGTTCGACTT CTTGATTGTC ATCGGCAGCA TCATTGACGT CATCCTCAGT 
GAAATTGATG ACCCAGACGA GAGCGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGG 
GTCATGAGGC TGATCAAGCT GCTGAGCCGG GCAGAGGGAG TCCGCACTCT CCTGTGGACG 
TTCATCAAGT CCTTTCAGGC CCTGCCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC 
ATCTACGCCG TCATCGGCAT GCAGATGTTT GGGAAGATCG CCATGGTGGA CGGGACCCAG 
ATAAACCGGA ACAACAACTT CCAGACCTTC CCACAAGCTG TGTTACTGCT CTTCAGGTGT 
GCGACAGGGG AGGCGTGGCA GGAGATCTTG CTGGCCTGCA GCTACGGCAA GTTGTGCGAC 
CCCGAGTCAG ACTACGCCCC GGGCGAGGAG TACACGTGTG GCACCAACTT CGCCTACTAC 
TATTTCATCA GCTTCTACAT GCTGTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCTGTC 
ATCATGGACA ACTTTGACTA CCTCACGCGG GACTGGTCCA TCCTGGGCCC TCATCACCTG 
GATGAGTTCA AGGCCATTTG GGCAGAGTAT GACCCAGAAG CCAAGGGCAG AATCAAACAC 
CTGGATGTGG TGACGCTGCT GAGACGCATC CAGCCCCCCC TGGGCTTTGG CAAGTTCTGC 
CCACACCGGG TGGCCTGCAA GCGGCTGGTG GGCATGAACA TGCCCTTGAA CAGCGACGGC 
ACCGTCACCT TCAACGCCAC GCTCTTCGCG CTGGTCCGCA CGGCCCTCAA GATCAAGACG 
GAAGGTAACT TTGAGCAGGC CAACGAGGAG CTGAGGGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCCT GGACCAGGTC ATTCCTCCAA TAGGAGATGA TGAGGTGACT 
GTAGGGAAGT TCTACGCCAC GTTTCTCATC CAGGAGCACT TCCGGAAGTT CATGAAGCGC 
CAGGAAGAAT ATTATGGGTA TCGGCCCAAG AAGGACACCA TACAGATCCA GGCTGGACTG 
CGGACCATTG AGGAAGAGGC AGCCCCAGAG ATCCGCCGTA CCATCTCAGG AGACCTGACA 
GCTGAAGAGG AGCTGGAGAG AGCCATGGTG GAGGCTGCGA TGGAGGAAGG GATATTCCGG 
AGGACCGGAG GCCTCTTTGG CCAGGTGGAC AACTTCCTGG AAAGGACCAA CTCCCTGCCC 
CCTGTCATGG CCAACCAGAG ACCCCTCCAG TTTGCTGAGA TAGAGATGGA AGAGCTGGAA 
TCACCCGTCT TCTTGGAGGA TTTCCCTCAA GATCCTAGAA CCAACCCCCT TGCTCGTGCC 
AATACCAACA ATGCCAATGC CAACGTTGCC CATGACAACA GTGACCATAG CAACAGCCAG 
GTGTTTTCCA GCATCCGCTA CGAGAGGGAG TTCCCTGGAG AGCCAGAGCC GCCTGCTGCC 
AGAGGAGGAG CCTTGGGCCA ACCCCGGAGG GTCCTGGGAC CCCACGGCAG GCCCTGTGTG 
GAAAAGCTGA AAGGACAGAG GACCCAGAGA GAAATGCCCA GACCCCTGGC GTCTCCCGCC 
CCCTGCCAGT GTGCCGGGCC GGAGCGTCCC CTGCCCGAGG AGCGGAGTTG CCCCCCGCCG 
GGGTCTCTGC CTCAGGGAGC GCCCCCCAGC AGGAGGCCCA CGGAGAGTGG CTGCAGGTGC 
CCGACCCCAG CTACGGCCCG GCTGGTTCGA GAGGCTCTGG TCCGAGGAGG CCTGGACACC 
TTGGCGGCCG ATGCCAGCTT CGTCATGGCA ACGGGCCAGG CCCTGGCAGA TGCCTGCCGG 
ATGGAACCCG AGGACGTGGA GGTGGCGGCA ACAGAGCTGC TCAAGAAGCA AGAGTCCCCA 
GAGGGCCGGG CCAGTGCGCT GGGACGCCGG AGCCGCGGGT CCTCGCTGGG CAGCCTTGAC 
CTGCATCTGG GCTCCCGGGA GACCCTGGTG CCCCCCAGGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005622323.1
CDS1..5565
Translation

Target ORF information:

RefSeq Version XM_005622266.3
Organism Canis lupus familiaris(dog)
Definition Canis lupus familiaris calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005622266.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGT CCTCGCCCCA GGACGAGGGC CTGAGGAAGA AACAGCCCAA GAAGCCGGTT 
CCGGAGATCC TGCCGAGGCC GCCCCGGGCC CTGTTCTGCT TGACCCTGCA GAACCCCCTG 
AGGAAGGCCT GCATCAACAT TGTGGAATGG AAACCCTTCG AGACAATCAT CTTGCTCACC 
ATCTTTGCCA ACTGCGTGGC TCTGGCTGTG TACCTGCCCA TGCCGGAAGA CGACAACAAC 
ACTCTCAACC TTGGCCTGGA GAAGCTGGAG TATTTCTTCC TCATTGTTTT CTCCATCGAA 
GCGGCCATGA AGATCATCGC CTACGGCTTC CTCTTCCACC AGGACGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGACTT CATCATTGTC TTCCTGGGGG TTTTCACCGT GATTCTGGAA 
CAGGTTAACG TCATCCAAAG CAACACAGCC CCGATGAGCA GCAAAGGGGC CGGCCTGGAT 
GTCAAGGCCC TGAGAGCCTT CCGTGTGCTC AGACCCCTCC GGCTGGTGTC AGGGGTGCCG 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCCCTGTT CCACATCGCC 
CTGCTCGTCC TCTTTATGGT CATCATCTAC GCCATCATCG GGCTGGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATTGGG ACAGATATCG TGGCCACGGT GGAGAACGAG 
AAGCCCTCAC CCTGTGCCAG GACGGGCTCG GGGCGCCCAT GCACCATCAA TGGCAGCGAG 
TGTCGGGGCG GCTGGCCGGG GCCCAACCAC GGCATCACCC ACTTCGACAA CTTTGGCTTC 
TCCATGCTCA CCGTGTACCA GTGCATCACC ATGGAGGGGT GGACCGATGT CCTCTACTGG 
GTCAACGATG CCATCGGGAA CGAGTGGCCC TGGATCTATT TTGTCACCCT CATTCTGCTG 
GGATCCTTCT TCATTCTCAA CCTGGTGCTG GGTGTCCTGA GCGGGGAATT CACTAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGAACCTTC CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GCGGCTACAT GAGCTGGATC ACACAGGGCG AGGTCATGGA CGTCGAGGAC 
TTGAGAGAAG GGAAGCTGTC TTTGGATGAA GGAGGCTCCG ACACAGAGAG CCTGTACGAG 
ATCGAGGGCC TGAACAAAAT CATCCAGTTC GTCCGCCACT GGAGGCAGTG GAACCGGATC 
TTTCGCTGGA AGTGCCATGA CGTGATCAAG TCCAGGGTCT TCTACTGGCT GGTGATCCTG 
GTCGTCGCCC TCAACACCCT GTCTATCGCC TCAGAGCACC ACAACCAGCC CCTGTGGCTG 
ACCCACTTGC AAGACGTGGC CAACCGGGTG CTGCTGGCGC TCTTCACCGT CGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTCG TGGAATCGGG CGCCATGAGC 
CCCCTGGGCA TCTCCGTGCT GCGCTGCATC CGCCTCCTGA GGATCTTCAA GATCACCAAG 
TACTGGACGT CCCTGAGCAA CCTGGTGGCG TCCCTGCTCA ACTCCATCCG CTCCATCGCC 
TCCCTGCTGC TGCTGCTCTT CCTCTTCATC ATCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTATGACTT CGAGGACACC GAGGTGCGGC GCAGCAACTT TGACAACTTC 
CCGCAGGCCC TCATCAGCGT CTTCCAGGTC CTGACGGGCG AGGACTGGAA CTCCATGATG 
TACAGCGGGA TCATGGCCTA CGGCGGCCCG TCCTACCCGG GGGTGCTCGT GTGCATTTAT 
TTCATCATTC TGTTCGTCTG TGGCAACTAT ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCGGAGGC AGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCGGAGGAG 
AGAAAGCGCA GGAAGATGTC CAAGGGTCTC CCAGATAAGT CAGAGGAGGA GAAGTCCGTG 
ATGGCCAAGA AGCTAGAGCA GAAACCCAAG GGTGAGGGCA TCCCTACCAC CGCCAAGCTG 
AAGATCGATG AGTTTGAATC GAATGTCAAC GAAGTGAAGG ATCCTTACCC CTCGGCTGAC 
TTCCCAGGCG ATGATGAGGA AGACGAGCCT GAGATCCCAG TGAGCCCCCG ACCACGGCCC 
CTGGCTGAGC TGCAGCTGAA AGAGAAGGCA GTGCCCATTC CAGAAGCCAG CTCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGTGTC CTGTGCCACC GCATTGTCAA CGCTACCTGG 
TTCACCAACT TCATCCTGCT CTTCATCCTG CTCAGCAGCG CCGCCCTGGC CGCTGAGGAC 
CCCATCCGGG CCGAGTCTGT GAGGAATCAG ATCCTTGGGT ATTTTGACAT CGCTTTCACC 
TCTGTTTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGCGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGC TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTTGAGTC CAGCACCATC TCCGTGGTGA AAATCCTGAG GGTGCTCAGG 
GTGCTCCGAC CCCTGCGGGC CATCAACAGA GCCAAGGGGC TGAAGCACGT GGTGCAGTGC 
GTGTTCGTGG CCATCCGCAC GATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCCT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTA 
TCCAAGATGA CAGAGCAGGA GTGCAGGGGC TATTACTACG TGTACAAGGA CGGGGACCCC 
ACGCAGATGG AGCTGCGTCC CCGCCAGTGG ATACACAGCG ACTTCCACTT TGACAACGTG 
CTCTCCGCCA TGATGTCACT CTTCACAGTG TCCACCTTTG AGGGATGGCC CCAGCTACTT 
TACCAGGCCA TAGACTCCTA CAAGGAGGAC ATGGGCCCCG TCTACAACAA CCGAGTGGAG 
ATGGCCATCT TCTTCATCAT CTACATCATC CTCATTGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTCG TCATTGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTACAG TACGCCCTGA AGGCCCGCCC GCTGAGATGC 
TATATCCCCA AAAATCCATA CCAGTACCAG GTGTGGTACG TTGTCACCTC CTCCTACTTC 
GAATACCTGA TGTTTGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCAG AGGAGATGAA CCACATCTCA GATATCCTCA ATGTGGCCTT CACCATCATC 
TTCACCCTGG AGATGGTCCT CAAACTCATG GCATTCAAGG CCAGGGGCTA TTTTGGAGAC 
CCGTGGAACG TGTTCGACTT CTTGATTGTC ATCGGCAGCA TCATTGACGT CATCCTCAGT 
GAAATTGATG ACCCAGACGA GAGCGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGG 
GTCATGAGGC TGATCAAGCT GCTGAGCCGG GCAGAGGGAG TCCGCACTCT CCTGTGGACG 
TTCATCAAGT CCTTTCAGGC CCTGCCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC 
ATCTACGCCG TCATCGGCAT GCAGATGTTT GGGAAGATCG CCATGGTGGA CGGGACCCAG 
ATAAACCGGA ACAACAACTT CCAGACCTTC CCACAAGCTG TGTTACTGCT CTTCAGGTGT 
GCGACAGGGG AGGCGTGGCA GGAGATCTTG CTGGCCTGCA GCTACGGCAA GTTGTGCGAC 
CCCGAGTCAG ACTACGCCCC GGGCGAGGAG TACACGTGTG GCACCAACTT CGCCTACTAC 
TATTTCATCA GCTTCTACAT GCTGTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCTGTC 
ATCATGGACA ACTTTGACTA CCTCACGCGG GACTGGTCCA TCCTGGGCCC TCATCACCTG 
GATGAGTTCA AGGCCATTTG GGCAGAGTAT GACCCAGAAG CCAAGGGCAG AATCAAACAC 
CTGGATGTGG TGACGCTGCT GAGACGCATC CAGCCCCCCC TGGGCTTTGG CAAGTTCTGC 
CCACACCGGG TGGCCTGCAA GCGGCTGGTG GGCATGAACA TGCCCTTGAA CAGCGACGGC 
ACCGTCACCT TCAACGCCAC GCTCTTCGCG CTGGTCCGCA CGGCCCTCAA GATCAAGACG 
GAAGGTAACT TTGAGCAGGC CAACGAGGAG CTGAGGGCCA TCATCAAGAA GATCTGGAAG 
AGAACCAGCA TGAAGCTCCT GGACCAGGTC ATTCCTCCAA TAGGAGATGA TGAGGTGACT 
GTAGGGAAGT TCTACGCCAC GTTTCTCATC CAGGAGCACT TCCGGAAGTT CATGAAGCGC 
CAGGAAGAAT ATTATGGGTA TCGGCCCAAG AAGGACACCA TACAGATCCA GGCTGGACTG 
CGGACCATTG AGGAAGAGGC AGCCCCAGAG ATCCGCCGTA CCATCTCAGG AGACCTGACA 
GCTGAAGAGG AGCTGGAGAG AGCCATGGTG GAGGCTGCGA TGGAGGAAGG GATATTCCGG 
AGGACCGGAG GCCTCTTTGG CCAGGTGGAC AACTTCCTGG AAAGGACCAA CTCCCTGCCC 
CCTGTCATGG CCAACCAGAG ACCCCTCCAG TTTGCTGAGA TAGAGATGGA AGAGCTGGAA 
TCACCCGTCT TCTTGGAGGA TTTCCCTCAA GATCCTAGAA CCAACCCCCT TGCTCGTGCC 
AATACCAACA ATGCCAATGC CAACGTTGCC CATGACAACA GTGACCATAG CAACAGCCAG 
GTGTTTTCCA GCATCCGCTA CGAGAGGGAG TTCCCTGGAG AGCCAGAGCC GCCTGCTGCC 
AGAGGAGGAG CCTTGGGCCA ACCCCGGAGG GTCCTGGGAC CCCACGGCAG GCCCTGTGTG 
GAAAAGCTGA AAGGACAGAG GACCCAGAGA GAAATGCCCA GACCCCTGGC GTCTCCCGCC 
CCCTGCCAGT GTGCCGGGCC GGAGCGTCCC CTGCCCGAGG AGCGGAGTTG CCCCCCGCCG 
GGGTCTCTGC CTCAGGGAGC GCCCCCCAGC AGGAGGCCCA CGGAGAGTGG CTGCAGGTGC 
CCGACCCCAG CTACGGCCCG GCTGGTTCGA GAGGCTCTGG TCCGAGGAGG CCTGGACACC 
TTGGCGGCCG ATGCCAGCTT CGTCATGGCA ACGGGCCAGG CCCTGGCAGA TGCCTGCCGG 
ATGGAACCCG AGGACGTGGA GGTGGCGGCA ACAGAGCTGC TCAAGAAGCA AGAGTCCCCA 
GAGGGCCGGG CCAGTGCGCT GGGACGCCGG AGCCGCGGGT CCTCGCTGGG CAGCCTTGAC 
CTGCATCTGG GCTCCCGGGA GACCCTGGTG CCCCCCAGGC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed