ORF cDNA Clones and Custom Clones » Species Summary » Gallus gallus » CACNA1S cDNA ORF clone

CACNA1S cDNA ORF clone, Gallus gallus(chicken)

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Summary

Gene Symbol	CACNA1S
Entrez Gene ID	395985
Full Name	calcium voltage-gated channel subunit alpha1 S
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 S Names voltage-dependent L-type calcium channel subunit alpha-1S L-type calcium channel, alpha 1 subunit calcium channel voltage-dependent L type alpha 1S calcium channel, voltage-dependent, L type, alpha 1S subunit voltage-gated calcium channel subunit alpha Cav1.1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025143674.1	XP_024999442.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
XM_015298692.1	XP_015154178.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
NM_001305148.1	NP_001292077.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform 2
XM_025143675.1	XP_024999443.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform X3
XM_015298692.2	XP_015154178.2	voltage-dependent L-type calcium channel subunit alpha-1S isoform X4
XM_025143672.1	XP_024999440.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
XM_025143673.1	XP_024999441.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
NM_001305147.1	NP_001292076.1	voltage-dependent L-type calcium channel subunit alpha-1S isoform 1

Homologies

	CACNA1S	XP_002802046.1
Rattus norvegicus (Norway rat)	Cacna1s	NP_446325.1
Homo sapiens (human)	CACNA1S	NP_000060.2
Pan troglodytes (chimpanzee)	CACNA1S	XP_525018.3
Canis lupus familiaris (dog)	CACNA1S	XP_848685.3
Bos taurus (cattle)	CACNA1S	XP_002694288.2
Mus musculus (house mouse)	Cacna1s	NP_001074492.1
Danio rerio (zebrafish)	cacna1sa	NP_001139622.1
Gallus gallus (chicken)	CACNA1S	XP_419259.3
Danio rerio (zebrafish)	cacna1sb	NP_999891.1

Bibliography

Related articles in PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

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The following CACNA1S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa147930	XM_025143672.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147930	XM_025143673.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147931	XM_025143674.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147932	XM_025143675.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa147933	XM_015298692.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa46362	XM_015298692.1 Latest version!	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa19560	NM_001305147.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa19561	NM_001305148.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1S ( XM_025143672.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024999440.1 Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.

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CloneID	OGa147930
Clone ID Related Accession (Same CDS sequence)	XM_025143672.1 , XM_025143673.1
Accession Version	XM_025143672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC 
AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC 
CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC 
CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC 
TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG 
TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC 
TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC 
CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG 
GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT 
GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA 
CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG 
TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT 
ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG 
GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT 
GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG 
GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG 
GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC 
ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC 
AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT 
GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG 
GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG 
GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG 
CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC 
CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGGTTGGA ATACGAGGAT 
GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT CCAGTGCGGG AGCCTGACCT CCCCATCCCT 
TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT GCCTCTGAGC ATCACCCAGC ATCGGGGCCA 
TCCCTCCATC CCCAGCAGGG CAGCACAGTG ACAGCAGCAG CATCCAGCTC AGAACACCGC 
TGGCATGAAA GCATTGACAC AAACACATCT TCTACCCCCG CCATCACCTT CCTCATTCAA 
GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT CTGGCCCGCG ACCCATCCTT TGTTGCAGTG 
ACCAGGGATG AGATGGCAGC CAGCAGCCAG ATGGAGATGG ACGAAGTGGA GAAAGCTGCA 
GTGGAGCTGC TACAGGGAAG AGAAGCACTG CAGGACACAG AAATCAGCTC TGCTCCCCGG 
GACACCTTGG CTGCATCAGC AGTCAGCACC CCGGGGCTCA GCGTGGCCGC ATCCCATCAG 
ACCAGCTCTG CTGCTCGCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024999440.1
CDS	1641..7304
Translation	MCPLACLAFLTGWGTGERGDKGQKHFSFGEDQCGARQPSPPPAHSVACEALACLAMCRDDVKKRQQKEKSKKPVPPVAPRPPRALFCLTLQNPLRKACISIVEWKPFEIIILLTIFANCVALAIYQPMPEDDTNVANSSLEKLEYVFLIFFAIEAMLKIIAYGFLFHTDAYLRNGWNVLDFSIVSLGLVTMTLEQINAKEGGSLGGKGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGQELFKGKMHKTCYYLGTDVIATVGSEKPAPCTTSGHGRHCSINGTECRGGWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDMKGYMDWITHAEVMDSDRARGEGMMPLDEGGSETESLYEIEGMNKWILYFRQWRRWNRMFRRKCRDVVKSKFFYWLVILLVALNTLSIASEHHFQPEWLTIVQDNANRVLLALFVAEMLLKMYALGLRQYFMSLFNRFDCFVVCAGVLEIILVELSTLSPLGISVLRCIRLLRIFKITRYWTSLSNLVASLLNSVRSIASLLLLLFLFIIVFALLGMQLFGGMYDFEDMEVRRSTFDNFPQALISVFQILTGEDWNSIMYNGIMAYGGPSFPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGYPEKSEDEKQMLAKKLEQKAKGEGIPTTAKLKVDEFESNVNEIKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKFRMLCHRIVNATWFTNFILLFILLSSISLAAEDPIRAESFRNQILGYFDIGFTSVFTVEIVLKMTAYGAFLHKGSFCRNSFNILDLLVVAVSLISMGFESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVVVTTLLQFMFACIGVQLFKGKFYSCTDPSKLTEKECRGHFINYVDGDPTQIELKERVWFHNAFHFNNVLSAMMSLFTVSTFEGWPELLYRAIDTNDENKGPIYNYRVEIAMFFIIYIILIAFFMMNIFVGFVIVTFQEQGESEYKNCELDKNQRQCVQYALKARPLRRYIPKNPYQYQIWYVVTSSYFEYLMFFLIMLNTICLGMQHYNQSAEMNHVSDILNVAFTVLFTLEMILKLMAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDTVLASSGGLYCLGGGCDSIDPDDNSRVSITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLDCSYGKRCDPESDYAEGEEYTCGTGFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVCMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRTIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKNPVEIQAGLRSIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIYRRTGGLFGQVDSFLEPSSPLQPHVASQRPLQFTEVGSEDLDSPVFLDDFPQEGNTNVNNANWQQGLEYEDEVLRRAVPRPPVREPDLPIPLERLRRRRGAASEHHPASGPSLHPQQGSTVTAAASSSEHRWHESIDTNTSSTPAITFLIQEALISGGLAALARDPSFVAVTRDEMAASSQMEMDEVEKAAVELLQGREALQDTEISSAPRDTLAASAVSTPGLSVAASHQTSSAARL

Target ORF information:

RefSeq Version	XM_025143672.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143672.1

ORF Insert Sequence:

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC 
AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC 
CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC 
CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC 
TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG 
TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC 
TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC 
CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG 
GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT 
GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA 
CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG 
TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT 
ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG 
GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT 
GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG 
GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG 
GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC 
ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC 
AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT 
GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG 
GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG 
GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG 
CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC 
CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGGTTGGA ATACGAGGAT 
GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT CCAGTGCGGG AGCCTGACCT CCCCATCCCT 
TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT GCCTCTGAGC ATCACCCAGC ATCGGGGCCA 
TCCCTCCATC CCCAGCAGGG CAGCACAGTG ACAGCAGCAG CATCCAGCTC AGAACACCGC 
TGGCATGAAA GCATTGACAC AAACACATCT TCTACCCCCG CCATCACCTT CCTCATTCAA 
GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT CTGGCCCGCG ACCCATCCTT TGTTGCAGTG 
ACCAGGGATG AGATGGCAGC CAGCAGCCAG ATGGAGATGG ACGAAGTGGA GAAAGCTGCA 
GTGGAGCTGC TACAGGGAAG AGAAGCACTG CAGGACACAG AAATCAGCTC TGCTCCCCGG 
GACACCTTGG CTGCATCAGC AGTCAGCACC CCGGGGCTCA GCGTGGCCGC ATCCCATCAG 
ACCAGCTCTG CTGCTCGCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1S ( XM_025143673.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024999441.1 Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA.

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CloneID	OGa147930
Clone ID Related Accession (Same CDS sequence)	XM_025143672.1 , XM_025143673.1
Accession Version	XM_025143673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC 
AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC 
CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC 
CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC 
TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG 
TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC 
TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC 
CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG 
GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT 
GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA 
CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG 
TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT 
ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG 
GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT 
GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG 
GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG 
GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC 
ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC 
AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT 
GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG 
GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG 
GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG 
CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC 
CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGGTTGGA ATACGAGGAT 
GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT CCAGTGCGGG AGCCTGACCT CCCCATCCCT 
TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT GCCTCTGAGC ATCACCCAGC ATCGGGGCCA 
TCCCTCCATC CCCAGCAGGG CAGCACAGTG ACAGCAGCAG CATCCAGCTC AGAACACCGC 
TGGCATGAAA GCATTGACAC AAACACATCT TCTACCCCCG CCATCACCTT CCTCATTCAA 
GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT CTGGCCCGCG ACCCATCCTT TGTTGCAGTG 
ACCAGGGATG AGATGGCAGC CAGCAGCCAG ATGGAGATGG ACGAAGTGGA GAAAGCTGCA 
GTGGAGCTGC TACAGGGAAG AGAAGCACTG CAGGACACAG AAATCAGCTC TGCTCCCCGG 
GACACCTTGG CTGCATCAGC AGTCAGCACC CCGGGGCTCA GCGTGGCCGC ATCCCATCAG 
ACCAGCTCTG CTGCTCGCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024999441.1
CDS	723..6386
Translation	MCPLACLAFLTGWGTGERGDKGQKHFSFGEDQCGARQPSPPPAHSVACEALACLAMCRDDVKKRQQKEKSKKPVPPVAPRPPRALFCLTLQNPLRKACISIVEWKPFEIIILLTIFANCVALAIYQPMPEDDTNVANSSLEKLEYVFLIFFAIEAMLKIIAYGFLFHTDAYLRNGWNVLDFSIVSLGLVTMTLEQINAKEGGSLGGKGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGQELFKGKMHKTCYYLGTDVIATVGSEKPAPCTTSGHGRHCSINGTECRGGWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDMKGYMDWITHAEVMDSDRARGEGMMPLDEGGSETESLYEIEGMNKWILYFRQWRRWNRMFRRKCRDVVKSKFFYWLVILLVALNTLSIASEHHFQPEWLTIVQDNANRVLLALFVAEMLLKMYALGLRQYFMSLFNRFDCFVVCAGVLEIILVELSTLSPLGISVLRCIRLLRIFKITRYWTSLSNLVASLLNSVRSIASLLLLLFLFIIVFALLGMQLFGGMYDFEDMEVRRSTFDNFPQALISVFQILTGEDWNSIMYNGIMAYGGPSFPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGYPEKSEDEKQMLAKKLEQKAKGEGIPTTAKLKVDEFESNVNEIKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKFRMLCHRIVNATWFTNFILLFILLSSISLAAEDPIRAESFRNQILGYFDIGFTSVFTVEIVLKMTAYGAFLHKGSFCRNSFNILDLLVVAVSLISMGFESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVVVTTLLQFMFACIGVQLFKGKFYSCTDPSKLTEKECRGHFINYVDGDPTQIELKERVWFHNAFHFNNVLSAMMSLFTVSTFEGWPELLYRAIDTNDENKGPIYNYRVEIAMFFIIYIILIAFFMMNIFVGFVIVTFQEQGESEYKNCELDKNQRQCVQYALKARPLRRYIPKNPYQYQIWYVVTSSYFEYLMFFLIMLNTICLGMQHYNQSAEMNHVSDILNVAFTVLFTLEMILKLMAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDTVLASSGGLYCLGGGCDSIDPDDNSRVSITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLDCSYGKRCDPESDYAEGEEYTCGTGFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVCMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRTIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKNPVEIQAGLRSIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIYRRTGGLFGQVDSFLEPSSPLQPHVASQRPLQFTEVGSEDLDSPVFLDDFPQEGNTNVNNANWQQGLEYEDEVLRRAVPRPPVREPDLPIPLERLRRRRGAASEHHPASGPSLHPQQGSTVTAAASSSEHRWHESIDTNTSSTPAITFLIQEALISGGLAALARDPSFVAVTRDEMAASSQMEMDEVEKAAVELLQGREALQDTEISSAPRDTLAASAVSTPGLSVAASHQTSSAARL

Target ORF information:

RefSeq Version	XM_025143673.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143673.1

ORF Insert Sequence:

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC 
AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC 
CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC 
CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC 
TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG 
TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC 
TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC 
CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG 
GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT 
GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA 
CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG 
TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT 
ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG 
GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT 
GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG 
GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG 
GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC 
ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC 
AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT 
GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG 
GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG 
GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG 
CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC 
CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGGTTGGA ATACGAGGAT 
GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT CCAGTGCGGG AGCCTGACCT CCCCATCCCT 
TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT GCCTCTGAGC ATCACCCAGC ATCGGGGCCA 
TCCCTCCATC CCCAGCAGGG CAGCACAGTG ACAGCAGCAG CATCCAGCTC AGAACACCGC 
TGGCATGAAA GCATTGACAC AAACACATCT TCTACCCCCG CCATCACCTT CCTCATTCAA 
GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT CTGGCCCGCG ACCCATCCTT TGTTGCAGTG 
ACCAGGGATG AGATGGCAGC CAGCAGCCAG ATGGAGATGG ACGAAGTGGA GAAAGCTGCA 
GTGGAGCTGC TACAGGGAAG AGAAGCACTG CAGGACACAG AAATCAGCTC TGCTCCCCGG 
GACACCTTGG CTGCATCAGC AGTCAGCACC CCGGGGCTCA GCGTGGCCGC ATCCCATCAG 
ACCAGCTCTG CTGCTCGCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1S ( XM_025143674.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024999442.1 Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X3, mRNA.

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CloneID	OGa147931
Clone ID Related Accession (Same CDS sequence)	XM_025143674.1
Accession Version	XM_025143674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATGACCC GGATGACAAC 
TCCCGTGTCT CCATCACGTT CTTCCGGCTG TTCCGAGTGA TGCGGCTGGT GAAGCTGCTG 
AGCCGGGGAG AAGGCGTCAG GACCCTCCTG TGGACCTTCA TCAAATCCTT CCAGGCTCTG 
CCCTATGTTG CCCTGCTGAT CGTGATGCTG TTCTTCATCT ATGCTGTGAT CGGGATGCAG 
ATGTTTGGGA AGATTGCCAT GGTGGACGGA ACCCAGATCA ACCGAAACAA CAACTTCCAA 
ACCTTCCCAC AAGCTGTGCT GCTGCTCTTC AGGTGTGCCA CGGGGGAGGC CTGGCAGGAG 
ATCCTGCTGG ACTGCAGCTA TGGGAAGCGG TGCGACCCTG AGTCTGACTA CGCGGAGGGC 
GAGGAGTACA CCTGTGGCAC GGGCTTTGCC TACTTCTATT TCATCAGCTT CTACATGCTC 
TGTGCCTTTC TGATCATTAA CCTTTTTGTG GCTGTCATCA TGGACAACTT TGACTACCTC 
ACACGTGACT GGTCCATCCT GGGGCCCCAC CACCTGGATG AGTTCAAACG GATCTGGGCT 
GAGTACGACC CTGAAGCCAA GGGCAGGATT AAGCACTTGG ATGTGGTGAC CCTGCTGAGA 
CGTATCCAAC CTCCCCTGGG CTTTGGGAAG TTCTGCCCTC ACCGTGTAGC TTGTAAGCGC 
CTGGTGTGCA TGAACATGCC CCTGAACAGC GATGGCACTG TCACCTTCAA TGCAACCCTC 
TTTGCGCTGG TGAGGACAGC CCTCAAAATC AAGACAGAAG GTAACTTTGA ACAGGCCAAT 
GAGGAGCTCA GAACCATTAT CAAAAAAATC TGGAAGCGAA CCAGTATGAA GCTTTTGGAC 
CAGGTCATCC CACCTATTGG AGATGATGAG GTGACAGTGG GCAAATTCTA TGCTACTTTC 
CTCATCCAAG AGCATTTCCG AAAGTTCATG AAGCGCCAGG AGGAGTACTA TGGGTACCGT 
CCCAAGAAGA ACCCTGTAGA GATCCAGGCT GGGCTGAGAA GCATTGAAGA AGAAGCTGCT 
CCTGAAATTC ATCGAGCCAT CTCTGGGGAC CTGACTGCTG AGGAGGAGCT GGAGAGGGCA 
ATGGTGGAAG CTGCCATGGA GGAGGGGATA TACAGGAGGA CGGGAGGTTT GTTTGGCCAG 
GTGGACAGCT TCCTGGAGCC GAGCAGCCCC CTGCAGCCCC ACGTGGCCAG CCAGAGGCCC 
CTGCAGTTCA CAGAGGTGGG CAGCGAAGAC CTCGATTCCC CCGTCTTCCT CGACGACTTC 
CCCCAGGAAG GCAACACCAA TGTCAACAAC GCCAACTGGC AGCAGGGGTT GGAATACGAG 
GATGAGGTGC TGAGGAGAGC AGTGCCCAGG CCTCCAGTGC GGGAGCCTGA CCTCCCCATC 
CCTTTGGAGA GGCTGCGGCG GAGGCGAGGA GCTGCCTCTG AGCATCACCC AGCATCGGGG 
CCATCCCTCC ATCCCCAGCA GGGCAGCACA GTGACAGCAG CAGCATCCAG CTCAGAACAC 
CGCTGGCATG AAAGCATTGA CACAAACACA TCTTCTACCC CCGCCATCAC CTTCCTCATT 
CAAGAGGCTC TGATCTCCGG AGGCCTCGCT GCTCTGGCCC GCGACCCATC CTTTGTTGCA 
GTGACCAGGG ATGAGATGGC AGCCAGCAGC CAGATGGAGA TGGACGAAGT GGAGAAAGCT 
GCAGTGGAGC TGCTACAGGG AAGAGAAGCA CTGCAGGACA CAGAAATCAG CTCTGCTCCC 
CGGGACACCT TGGCTGCATC AGCAGTCAGC ACCCCGGGGC TCAGCGTGGC CGCATCCCAT 
CAGACCAGCT CTGCTGCTCG CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024999442.1
CDS	1641..7247
Translation	MCPLACLAFLTGWGTGERGDKGQKHFSFGEDQCGARQPSPPPAHSVACEALACLAMCRDDVKKRQQKEKSKKPVPPVAPRPPRALFCLTLQNPLRKACISIVEWKPFEIIILLTIFANCVALAIYQPMPEDDTNVANSSLEKLEYVFLIFFAIEAMLKIIAYGFLFHTDAYLRNGWNVLDFSIVSLGLVTMTLEQINAKEGGSLGGKGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGQELFKGKMHKTCYYLGTDVIATVGSEKPAPCTTSGHGRHCSINGTECRGGWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDMKGYMDWITHAEVMDSDRARGEGMMPLDEGGSETESLYEIEGMNKWILYFRQWRRWNRMFRRKCRDVVKSKFFYWLVILLVALNTLSIASEHHFQPEWLTIVQDNANRVLLALFVAEMLLKMYALGLRQYFMSLFNRFDCFVVCAGVLEIILVELSTLSPLGISVLRCIRLLRIFKITRYWTSLSNLVASLLNSVRSIASLLLLLFLFIIVFALLGMQLFGGMYDFEDMEVRRSTFDNFPQALISVFQILTGEDWNSIMYNGIMAYGGPSFPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGYPEKSEDEKQMLAKKLEQKAKGEGIPTTAKLKVDEFESNVNEIKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKFRMLCHRIVNATWFTNFILLFILLSSISLAAEDPIRAESFRNQILGYFDIGFTSVFTVEIVLKMTAYGAFLHKGSFCRNSFNILDLLVVAVSLISMGFESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVVVTTLLQFMFACIGVQLFKGKFYSCTDPSKLTEKECRGHFINYVDGDPTQIELKERVWFHNAFHFNNVLSAMMSLFTVSTFEGWPELLYRAIDTNDENKGPIYNYRVEIAMFFIIYIILIAFFMMNIFVGFVIVTFQEQGESEYKNCELDKNQRQCVQYALKARPLRRYIPKNPYQYQIWYVVTSSYFEYLMFFLIMLNTICLGMQHYNQSAEMNHVSDILNVAFTVLFTLEMILKLMAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDDPDDNSRVSITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLDCSYGKRCDPESDYAEGEEYTCGTGFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVCMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRTIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKNPVEIQAGLRSIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIYRRTGGLFGQVDSFLEPSSPLQPHVASQRPLQFTEVGSEDLDSPVFLDDFPQEGNTNVNNANWQQGLEYEDEVLRRAVPRPPVREPDLPIPLERLRRRRGAASEHHPASGPSLHPQQGSTVTAAASSSEHRWHESIDTNTSSTPAITFLIQEALISGGLAALARDPSFVAVTRDEMAASSQMEMDEVEKAAVELLQGREALQDTEISSAPRDTLAASAVSTPGLSVAASHQTSSAARL

Target ORF information:

RefSeq Version	XM_025143674.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143674.1

ORF Insert Sequence:

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATGACCC GGATGACAAC 
TCCCGTGTCT CCATCACGTT CTTCCGGCTG TTCCGAGTGA TGCGGCTGGT GAAGCTGCTG 
AGCCGGGGAG AAGGCGTCAG GACCCTCCTG TGGACCTTCA TCAAATCCTT CCAGGCTCTG 
CCCTATGTTG CCCTGCTGAT CGTGATGCTG TTCTTCATCT ATGCTGTGAT CGGGATGCAG 
ATGTTTGGGA AGATTGCCAT GGTGGACGGA ACCCAGATCA ACCGAAACAA CAACTTCCAA 
ACCTTCCCAC AAGCTGTGCT GCTGCTCTTC AGGTGTGCCA CGGGGGAGGC CTGGCAGGAG 
ATCCTGCTGG ACTGCAGCTA TGGGAAGCGG TGCGACCCTG AGTCTGACTA CGCGGAGGGC 
GAGGAGTACA CCTGTGGCAC GGGCTTTGCC TACTTCTATT TCATCAGCTT CTACATGCTC 
TGTGCCTTTC TGATCATTAA CCTTTTTGTG GCTGTCATCA TGGACAACTT TGACTACCTC 
ACACGTGACT GGTCCATCCT GGGGCCCCAC CACCTGGATG AGTTCAAACG GATCTGGGCT 
GAGTACGACC CTGAAGCCAA GGGCAGGATT AAGCACTTGG ATGTGGTGAC CCTGCTGAGA 
CGTATCCAAC CTCCCCTGGG CTTTGGGAAG TTCTGCCCTC ACCGTGTAGC TTGTAAGCGC 
CTGGTGTGCA TGAACATGCC CCTGAACAGC GATGGCACTG TCACCTTCAA TGCAACCCTC 
TTTGCGCTGG TGAGGACAGC CCTCAAAATC AAGACAGAAG GTAACTTTGA ACAGGCCAAT 
GAGGAGCTCA GAACCATTAT CAAAAAAATC TGGAAGCGAA CCAGTATGAA GCTTTTGGAC 
CAGGTCATCC CACCTATTGG AGATGATGAG GTGACAGTGG GCAAATTCTA TGCTACTTTC 
CTCATCCAAG AGCATTTCCG AAAGTTCATG AAGCGCCAGG AGGAGTACTA TGGGTACCGT 
CCCAAGAAGA ACCCTGTAGA GATCCAGGCT GGGCTGAGAA GCATTGAAGA AGAAGCTGCT 
CCTGAAATTC ATCGAGCCAT CTCTGGGGAC CTGACTGCTG AGGAGGAGCT GGAGAGGGCA 
ATGGTGGAAG CTGCCATGGA GGAGGGGATA TACAGGAGGA CGGGAGGTTT GTTTGGCCAG 
GTGGACAGCT TCCTGGAGCC GAGCAGCCCC CTGCAGCCCC ACGTGGCCAG CCAGAGGCCC 
CTGCAGTTCA CAGAGGTGGG CAGCGAAGAC CTCGATTCCC CCGTCTTCCT CGACGACTTC 
CCCCAGGAAG GCAACACCAA TGTCAACAAC GCCAACTGGC AGCAGGGGTT GGAATACGAG 
GATGAGGTGC TGAGGAGAGC AGTGCCCAGG CCTCCAGTGC GGGAGCCTGA CCTCCCCATC 
CCTTTGGAGA GGCTGCGGCG GAGGCGAGGA GCTGCCTCTG AGCATCACCC AGCATCGGGG 
CCATCCCTCC ATCCCCAGCA GGGCAGCACA GTGACAGCAG CAGCATCCAG CTCAGAACAC 
CGCTGGCATG AAAGCATTGA CACAAACACA TCTTCTACCC CCGCCATCAC CTTCCTCATT 
CAAGAGGCTC TGATCTCCGG AGGCCTCGCT GCTCTGGCCC GCGACCCATC CTTTGTTGCA 
GTGACCAGGG ATGAGATGGC AGCCAGCAGC CAGATGGAGA TGGACGAAGT GGAGAAAGCT 
GCAGTGGAGC TGCTACAGGG AAGAGAAGCA CTGCAGGACA CAGAAATCAG CTCTGCTCCC 
CGGGACACCT TGGCTGCATC AGCAGTCAGC ACCCCGGGGC TCAGCGTGGC CGCATCCCAT 
CAGACCAGCT CTGCTGCTCG CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1S ( XM_025143675.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_024999443.1 Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X4, mRNA.

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CloneID	OGa147932
Clone ID Related Accession (Same CDS sequence)	XM_025143675.1
Accession Version	XM_025143675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC 
AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC 
CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC 
CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC 
TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG 
TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC 
TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC 
CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG 
GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT 
GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA 
CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG 
TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT 
ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG 
GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT 
GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG 
GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG 
GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC 
ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC 
AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT 
GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG 
GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG 
GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG 
CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC 
CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGAGTGGA GAAGATGGGG 
AGGGCTGCAT CCTGGGCTCA AAGTGGTGGT CCCTGCTCGG CTGACCCCTC TGCCTGCCCC 
TGCCAGGTTG GAATACGAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024999443.1
CDS	1641..6884
Translation	MCPLACLAFLTGWGTGERGDKGQKHFSFGEDQCGARQPSPPPAHSVACEALACLAMCRDDVKKRQQKEKSKKPVPPVAPRPPRALFCLTLQNPLRKACISIVEWKPFEIIILLTIFANCVALAIYQPMPEDDTNVANSSLEKLEYVFLIFFAIEAMLKIIAYGFLFHTDAYLRNGWNVLDFSIVSLGLVTMTLEQINAKEGGSLGGKGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGQELFKGKMHKTCYYLGTDVIATVGSEKPAPCTTSGHGRHCSINGTECRGGWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDMKGYMDWITHAEVMDSDRARGEGMMPLDEGGSETESLYEIEGMNKWILYFRQWRRWNRMFRRKCRDVVKSKFFYWLVILLVALNTLSIASEHHFQPEWLTIVQDNANRVLLALFVAEMLLKMYALGLRQYFMSLFNRFDCFVVCAGVLEIILVELSTLSPLGISVLRCIRLLRIFKITRYWTSLSNLVASLLNSVRSIASLLLLLFLFIIVFALLGMQLFGGMYDFEDMEVRRSTFDNFPQALISVFQILTGEDWNSIMYNGIMAYGGPSFPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGYPEKSEDEKQMLAKKLEQKAKGEGIPTTAKLKVDEFESNVNEIKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKFRMLCHRIVNATWFTNFILLFILLSSISLAAEDPIRAESFRNQILGYFDIGFTSVFTVEIVLKMTAYGAFLHKGSFCRNSFNILDLLVVAVSLISMGFESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVVVTTLLQFMFACIGVQLFKGKFYSCTDPSKLTEKECRGHFINYVDGDPTQIELKERVWFHNAFHFNNVLSAMMSLFTVSTFEGWPELLYRAIDTNDENKGPIYNYRVEIAMFFIIYIILIAFFMMNIFVGFVIVTFQEQGESEYKNCELDKNQRQCVQYALKARPLRRYIPKNPYQYQIWYVVTSSYFEYLMFFLIMLNTICLGMQHYNQSAEMNHVSDILNVAFTVLFTLEMILKLMAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDTVLASSGGLYCLGGGCDSIDPDDNSRVSITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLDCSYGKRCDPESDYAEGEEYTCGTGFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVCMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRTIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKNPVEIQAGLRSIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIYRRTGGLFGQVDSFLEPSSPLQPHVASQRPLQFTEVGSEDLDSPVFLDDFPQEGNTNVNNANWQQGVEKMGRAASWAQSGGPCSADPSACPCQVGIRG

Target ORF information:

RefSeq Version	XM_025143675.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025143675.1

ORF Insert Sequence:

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC 
AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC 
CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC 
CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC 
TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG 
TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC 
TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC 
CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG 
GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT 
GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA 
CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG 
TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT 
ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG 
GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT 
GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG 
GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG 
GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC 
ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC 
AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT 
GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG 
GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG 
GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG 
CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC 
CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGAGTGGA GAAGATGGGG 
AGGGCTGCAT CCTGGGCTCA AAGTGGTGGT CCCTGCTCGG CTGACCCCTC TGCCTGCCCC 
TGCCAGGTTG GAATACGAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1S ( XM_015298692.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154178.2 Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X5, mRNA.

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CloneID	OGa147933
Clone ID Related Accession (Same CDS sequence)	XM_015298692.2
Accession Version	XM_015298692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC 
AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC 
CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC 
CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC 
TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG 
TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC 
TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC 
CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG 
GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT 
GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA 
CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG 
TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT 
ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG 
GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT 
GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG 
GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG 
GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC 
ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC 
AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT 
GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG 
GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG 
GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG 
CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC 
CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154178.2
CDS	1641..6791
Translation	MCPLACLAFLTGWGTGERGDKGQKHFSFGEDQCGARQPSPPPAHSVACEALACLAMCRDDVKKRQQKEKSKKPVPPVAPRPPRALFCLTLQNPLRKACISIVEWKPFEIIILLTIFANCVALAIYQPMPEDDTNVANSSLEKLEYVFLIFFAIEAMLKIIAYGFLFHTDAYLRNGWNVLDFSIVSLGLVTMTLEQINAKEGGSLGGKGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGQELFKGKMHKTCYYLGTDVIATVGSEKPAPCTTSGHGRHCSINGTECRGGWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDMKGYMDWITHAEVMDSDRARGEGMMPLDEGGSETESLYEIEGMNKWILYFRQWRRWNRMFRRKCRDVVKSKFFYWLVILLVALNTLSIASEHHFQPEWLTIVQDNANRVLLALFVAEMLLKMYALGLRQYFMSLFNRFDCFVVCAGVLEIILVELSTLSPLGISVLRCIRLLRIFKITRYWTSLSNLVASLLNSVRSIASLLLLLFLFIIVFALLGMQLFGGMYDFEDMEVRRSTFDNFPQALISVFQILTGEDWNSIMYNGIMAYGGPSFPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGYPEKSEDEKQMLAKKLEQKAKGEGIPTTAKLKVDEFESNVNEIKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKFRMLCHRIVNATWFTNFILLFILLSSISLAAEDPIRAESFRNQILGYFDIGFTSVFTVEIVLKMTAYGAFLHKGSFCRNSFNILDLLVVAVSLISMGFESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVVVTTLLQFMFACIGVQLFKGKFYSCTDPSKLTEKECRGHFINYVDGDPTQIELKERVWFHNAFHFNNVLSAMMSLFTVSTFEGWPELLYRAIDTNDENKGPIYNYRVEIAMFFIIYIILIAFFMMNIFVGFVIVTFQEQGESEYKNCELDKNQRQCVQYALKARPLRRYIPKNPYQYQIWYVVTSSYFEYLMFFLIMLNTICLGMQHYNQSAEMNHVSDILNVAFTVLFTLEMILKLMAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDTVLASSGGLYCLGGGCDSIDPDDNSRVSITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLDCSYGKRCDPESDYAEGEEYTCGTGFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVCMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRTIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKNPVEIQAGLRSIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIYRRTGGLFGQVDSFLEPSSPLQPHVASQRPLQFTEVGSEDLDSPVFLDDFPQEGNTNVNNANWQQGA

Target ORF information:

RefSeq Version	XM_015298692.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298692.2

ORF Insert Sequence:

ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA 
CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT 
GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG 
CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC 
ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT 
GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG 
GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA 
GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC 
TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG 
GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG 
CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT 
ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC 
TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT 
GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC 
CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC 
AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC 
ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG 
CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG 
CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG 
TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG 
ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG 
GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT 
CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG 
GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC 
ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC 
CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG 
CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC 
CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC 
TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG 
GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC 
TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG 
GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC 
CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG 
GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG 
AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG 
AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA 
GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA 
GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT 
GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT 
GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG 
AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT 
CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC 
ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC 
AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC 
TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC 
AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC 
ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT 
AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT 
GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG 
CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT 
AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG 
GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC 
ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT 
GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC 
ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC 
CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT 
GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA 
TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC 
ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC 
GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG 
CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC 
ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC 
AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC 
CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC 
CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC 
TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG 
TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC 
TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC 
CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG 
GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT 
GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA 
CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG 
TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT 
ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG 
GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT 
GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG 
GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG 
GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC 
ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC 
AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT 
GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG 
GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG 
GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG 
CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC 
CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1S ( XM_015298692.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015154178.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), transcript variant X1, mRNA.

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CloneID	OGa46362
Clone ID Related Accession (Same CDS sequence)	XM_015298692.1
Accession Version	XM_015298692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456028.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG 
AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC 
CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT 
GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT 
GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT 
CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG 
ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT 
GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA 
AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA 
CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG 
AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG 
TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT 
GCCATGCTGA CCGTCTTCCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG 
GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT 
GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG 
CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG 
GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT 
GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA 
ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC 
CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG 
CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG 
CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC 
GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG 
TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC 
AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT 
GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTGCT GGGCATGCAG 
CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC 
TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA 
ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC 
TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT 
GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG 
GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG 
ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG 
TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA 
GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT 
CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC 
TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT 
TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG 
GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC 
ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC 
AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT 
CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG 
AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG 
TGTGTGTTCG TGGCCATCAG AACCATTGGT AACATTGTGA TTGTTACCAC TTTGCTGCAG 
TTCATGTTTG CCTGCATTGG AGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT 
CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC 
CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT 
GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG 
CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG 
GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC 
TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT 
GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG 
CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC 
TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT 
TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC 
CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG 
GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC 
AGCGAGATTG ATACTGTTCT TGCATCCAGT GGTGGATTGT ACTGCCTCGG CGGAGGTTGT 
GATAGCATTG ACCCGGATGA CAACTCCCGT GTCTCCATCA CGTTCTTCCG GCTGTTCCGA 
GTGATGCGGC TGGTGAAGCT GCTGAGCCGG GGAGAAGGCG TCAGGACCCT CCTGTGGACC 
TTCATCAAAT CCTTCCAGGC TCTGCCCTAT GTTGCCCTGC TGATCGTGAT GCTGTTCTTC 
ATCTATGCTG TGATCGGGAT GCAGATGTTT GGGAAGATTG CCATGGTGGA CGGAACCCAG 
ATCAACCGAA ACAACAACTT CCAAACCTTC CCCCAAGCTG TGCTGCTGCT CTTCAGGTGT 
GCCACGGGGG AGGCCTGGCA GGAGATCCTG CTGGACTGCA GCTATGGGAA GCGGTGCGAC 
CCTGAGTCTG ACTACGCGGA GGGCGAGGAG TACACCTGTG GCACGGGCTT TGCCTACTTC 
TATTTCATCA GCTTCTACAT GCTCTGTGCC TTTCTGATCA TTAACCTTTT TGTGGCTGTC 
ATCATGGACA ACTTTGACTA CCTGACACGT GACTGGTCCA TCCTGGGGCC CCACCACCTG 
GATGAGTTCA AACGGATCTG GGCTGAGTAC GACCCTGAAG CCAAGGGCAG GATTAAGCAC 
TTGGATGTGG TGACCCTGCT GAGACGTATC CAACCTCCCC TGGGCTTTGG GAAGTTCTGC 
CCTCACCGTG TAGCTTGTAA GCGCCTGGTG TGCATGAACA TGCCCCTGAA CAGCGATGGC 
ACTGTCACCT TCAATGCAAC CCTCTTTGCG CTGGTGAGGA CAGCCCTCAA AATCAAGACA 
GAAGGTAACT TTGAACAGGC CAATGAGGAG CTCAGAACCA TTATCAAAAA AATCTGGAAG 
CGAACCAGTA TGAAGCTTTT GGACCAGGTC ATCCCACCTA TTGGAGATGA TGAGGTGACA 
GTGGGCAAAT TCTATGCTAC TTTCCTCATC CAAGAGCATT TCCGAAAGTT CATGAAGCGC 
CAGGAGGAGT ACTATGGGTA CCGTCCCAAG AAGAACCCTG TAGAGATCCA GGCTGGGCTG 
AGAAGCATTG AAGAAGAAGC TGCTCCTGAA ATTCATCGAG CCATCTCTGG GGACCTGACT 
GCTGAGGAGG AGCTGGAGAG GGCAATGGTG GAAGCTGCCA TGGAGGAGGG GATATACAGG 
AGGACGGGAG GTTTGTTTGG CCAGGTGGAC AGCTTCCTGG AGCCGAGCAG CCCCCTGCAG 
CCCCACGTGG CCAGCCAGAG GCCCCTGCAG TTCACAGAGG TGGGCAGCGA AGACCTCGAT 
TCCCCCGTCT TCCTCGACGA CTTCCCCCAG GAAGGCAACA CCAATGTCAA CAACGCCAAC 
TGGCAGCAGG GAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015154178.1
CDS	153..5150
Translation	MEPASHQDDVKKRQQKEKSKKPVPPVAPRPPRALFCLTLQNPLRKACISIVEWKPFEIIILLTIFANCVALAIYQPMPEDDTNVANSSLEKLEYVFLIFFAIEAMLKIIAYGFLFHTDAYLRNGWNVLDFSIVSLGLVTMTLEQINAKEGGSLGGKGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGQELFKGKMHKTCYYLGTDVIATVGSEKPAPCTTSGHGRHCSINGTECRGGWPGPNNGITHFDNFGFAMLTVFQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDMKGYMDWITHAEVMDSDRARGEGMMPLDEGGSETESLYEIEGMNKWILYFRQWRRWNRMFRRKCRDVVKSKFFYWLVILLVALNTLSIASEHHFQPEWLTIVQDNANRVLLALFVAEMLLKMYALGLRQYFMSLFNRFDCFVVCAGVLEIILVELSTLSPLGISVLRCIRLLRIFKITRYWTSLSNLVASLLNSVRSIASLLLLLFLFIIVFALLGMQLFGGMYDFEDMEVRRSTFDNFPQALISVFQILTGEDWNSIMYNGIMAYGGPSFPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGYPEKSEDEKQMLAKKLEQKAKGEGIPTTAKLKVDEFESNVNEIKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKFRMLCHRIVNATWFTNFILLFILLSSISLAAEDPIRAESFRNQILGYFDIGFTSVFTVEIVLKMTAYGAFLHKGSFCRNSFNILDLLVVAVSLISMGFESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKFYSCTDPSKLTEKECRGHFINYVDGDPTQIELKERVWFHNAFHFNNVLSAMMSLFTVSTFEGWPELLYRAIDTNDENKGPIYNYRVEIAMFFIIYIILIAFFMMNIFVGFVIVTFQEQGESEYKNCELDKNQRQCVQYALKARPLRRYIPKNPYQYQIWYVVTSSYFEYLMFFLIMLNTICLGMQHYNQSAEMNHVSDILNVAFTVLFTLEMILKLMAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDTVLASSGGLYCLGGGCDSIDPDDNSRVSITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLDCSYGKRCDPESDYAEGEEYTCGTGFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVCMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRTIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKNPVEIQAGLRSIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIYRRTGGLFGQVDSFLEPSSPLQPHVASQRPLQFTEVGSEDLDSPVFLDDFPQEGNTNVNNANWQQGA

Target ORF information:

RefSeq Version	XM_015298692.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015298692.1

ORF Insert Sequence:

ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG 
AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC 
CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT 
GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT 
GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT 
CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG 
ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT 
GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA 
AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA 
CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG 
AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG 
TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT 
GCCATGCTGA CCGTCTTCCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG 
GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT 
GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG 
CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG 
GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT 
GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA 
ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC 
CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG 
CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG 
CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC 
GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG 
TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC 
AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT 
GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTGCT GGGCATGCAG 
CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC 
TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA 
ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC 
TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT 
GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG 
GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG 
ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG 
TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA 
GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT 
CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC 
TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT 
TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG 
GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC 
ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC 
AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT 
CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG 
AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG 
TGTGTGTTCG TGGCCATCAG AACCATTGGT AACATTGTGA TTGTTACCAC TTTGCTGCAG 
TTCATGTTTG CCTGCATTGG AGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT 
CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC 
CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT 
GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG 
CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG 
GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC 
TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT 
GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG 
CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC 
TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT 
TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC 
CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG 
GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC 
AGCGAGATTG ATACTGTTCT TGCATCCAGT GGTGGATTGT ACTGCCTCGG CGGAGGTTGT 
GATAGCATTG ACCCGGATGA CAACTCCCGT GTCTCCATCA CGTTCTTCCG GCTGTTCCGA 
GTGATGCGGC TGGTGAAGCT GCTGAGCCGG GGAGAAGGCG TCAGGACCCT CCTGTGGACC 
TTCATCAAAT CCTTCCAGGC TCTGCCCTAT GTTGCCCTGC TGATCGTGAT GCTGTTCTTC 
ATCTATGCTG TGATCGGGAT GCAGATGTTT GGGAAGATTG CCATGGTGGA CGGAACCCAG 
ATCAACCGAA ACAACAACTT CCAAACCTTC CCCCAAGCTG TGCTGCTGCT CTTCAGGTGT 
GCCACGGGGG AGGCCTGGCA GGAGATCCTG CTGGACTGCA GCTATGGGAA GCGGTGCGAC 
CCTGAGTCTG ACTACGCGGA GGGCGAGGAG TACACCTGTG GCACGGGCTT TGCCTACTTC 
TATTTCATCA GCTTCTACAT GCTCTGTGCC TTTCTGATCA TTAACCTTTT TGTGGCTGTC 
ATCATGGACA ACTTTGACTA CCTGACACGT GACTGGTCCA TCCTGGGGCC CCACCACCTG 
GATGAGTTCA AACGGATCTG GGCTGAGTAC GACCCTGAAG CCAAGGGCAG GATTAAGCAC 
TTGGATGTGG TGACCCTGCT GAGACGTATC CAACCTCCCC TGGGCTTTGG GAAGTTCTGC 
CCTCACCGTG TAGCTTGTAA GCGCCTGGTG TGCATGAACA TGCCCCTGAA CAGCGATGGC 
ACTGTCACCT TCAATGCAAC CCTCTTTGCG CTGGTGAGGA CAGCCCTCAA AATCAAGACA 
GAAGGTAACT TTGAACAGGC CAATGAGGAG CTCAGAACCA TTATCAAAAA AATCTGGAAG 
CGAACCAGTA TGAAGCTTTT GGACCAGGTC ATCCCACCTA TTGGAGATGA TGAGGTGACA 
GTGGGCAAAT TCTATGCTAC TTTCCTCATC CAAGAGCATT TCCGAAAGTT CATGAAGCGC 
CAGGAGGAGT ACTATGGGTA CCGTCCCAAG AAGAACCCTG TAGAGATCCA GGCTGGGCTG 
AGAAGCATTG AAGAAGAAGC TGCTCCTGAA ATTCATCGAG CCATCTCTGG GGACCTGACT 
GCTGAGGAGG AGCTGGAGAG GGCAATGGTG GAAGCTGCCA TGGAGGAGGG GATATACAGG 
AGGACGGGAG GTTTGTTTGG CCAGGTGGAC AGCTTCCTGG AGCCGAGCAG CCCCCTGCAG 
CCCCACGTGG CCAGCCAGAG GCCCCTGCAG TTCACAGAGG TGGGCAGCGA AGACCTCGAT 
TCCCCCGTCT TCCTCGACGA CTTCCCCCAG GAAGGCAACA CCAATGTCAA CAACGCCAAC 
TGGCAGCAGG GAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1S ( NM_001305147.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001292076.1 Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 1, mRNA.

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CloneID	OGa19560
Clone ID Related Accession (Same CDS sequence)	NM_001305147.1
Accession Version	NM_001305147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-11
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000168.1. On Feb 25, 2015 this sequence version replaced XM_419259.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on protein alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201367, SAMEA2201368 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG 
AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC 
CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT 
GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT 
GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT 
CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG 
ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT 
GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA 
AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA 
CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG 
AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG 
TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT 
GCCATGCTGA CTGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG 
GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT 
GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG 
CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG 
GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT 
GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA 
ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC 
CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG 
CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG 
CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC 
GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG 
TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC 
AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT 
GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTACT GGGCATGCAG 
CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC 
TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA 
ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC 
TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT 
GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG 
GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG 
ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG 
TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA 
GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT 
CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC 
TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT 
TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG 
GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC 
ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC 
AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT 
CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG 
AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG 
TGTGTGTTCG TGGCCATCAA GACCATTGGT AACATTGTGG TCGTTACGAC GTTGCTGCAG 
TTCATGTTTG CCTGCATTGG GGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT 
CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC 
CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT 
GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG 
CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG 
GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC 
TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT 
GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG 
CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC 
TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT 
TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC 
CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG 
GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC 
AGCGAGATTG ATACTGTTCT TGCATCCAGT GGTGGATTGT ACTGCCTCGG CGGAGGTTGT 
GATAGCATTG ACCCGGATGA CAACTCCCGT GTCTCCATCA CGTTCTTCCG GCTGTTCCGA 
GTGATGCGGC TGGTGAAGCT GCTGAGCCGG GGAGAAGGCG TCAGGACCCT CCTGTGGACC 
TTCATCAAAT CCTTCCAGGC TCTGCCCTAT GTTGCCCTGC TGATCGTGAT GCTGTTCTTC 
ATCTATGCTG TGATCGGGAT GCAGATGTTT GGGAAGATTG CCATGGTGGA CGGAACCCAG 
ATCAACCGAA ACAACAACTT CCAAACCTTC CCACAAGCTG TGCTGCTGCT CTTCAGGTGT 
GCCACGGGGG AGGCCTGGCA GGAGATCCTG CTGGACTGCA GCTATGGGAA GCGGTGCGAC 
CCTGAGTCTG ACTACGCGGA GGGCGAGGAG TACACCTGTG GCACGGGCTT TGCCTACTTC 
TATTTCATCA GCTTCTACAT GCTCTGTGCC TTTCTGATCA TTAACCTTTT TGTGGCTGTC 
ATCATGGACA ACTTTGACTA CCTCACACGT GACTGGTCCA TCCTGGGGCC CCACCACCTG 
GATGAGTTCA AACGGATCTG GGCTGAGTAC GACCCTGAAG CCAAGGGCAG GATTAAGCAC 
TTGGATGTGG TGACCCTGCT GAGACGTATC CAACCTCCCC TGGGCTTTGG GAAGTTCTGC 
CCTCACCGTG TAGCTTGTAA GCGCCTGGTG TGCATGAACA TGCCCCTGAA CAGCGATGGC 
ACTGTCACCT TCAATGCAAC CCTCTTTGCG CTGGTGAGGA CAGCCCTCAA AATCAAGACA 
GAAGGTAACT TTGAACAGGC CAATGAGGAG CTCAGAACCA TTATCAAAAA AATCTGGAAG 
CGAACCAGTA TGAAGCTTTT GGACCAGGTC ATCCCACCTA TTGGAGATGA TGAGGTGACA 
GTGGGCAAAT TCTATGCTAC TTTCCTCATC CAAGAGCATT TCCGAAAGTT CATGAAGCGC 
CAGGAGGAGT ACTATGGGTA CCGTCCCAAG AAGAACCCTG TAGAGATCCA GGCTGGGCTG 
AGAAGCATTG AAGAAGAAGC TGCTCCTGAA ATTCATCGAG CCATCTCTGG GGACCTGACT 
GCTGAGGAGG AGCTGGAGAG GGCAATGGTG GAAGCTGCCA TGGAGGAGGG GATATACAGG 
AGGACGGGAG GTTTGTTTGG CCAGGTGGAC AGCTTCCTGG AGCCGAGCAG CCCCCTGCAG 
CCCCACGTGG CCAGCCAGAG GCCCCTGCAG TTCACAGAGG TGGGCAGCGA AGACCTCGAT 
TCCCCCGTCT TCCTCGACGA CTTCCCCCAG GAAGGCAACA CCAATGTCAA CAACGCCAAC 
TGGCAGCAGG GGTTGGAATA CGAGGATGAG GTGCTGAGGA GAGCAGTGCC CAGGCCTCCA 
GTGCGGGAGC CTGACCTCCC CATCCCTTTG GAGAGGCTGC GGCGGAGGCG AGGAGCTGCC 
TCTGAGCATC ACCCAGCATC GGGGCCATCC CTCCATCCCC AGCAGGGCAG CACAGTGACA 
GCAGCAGCAT CCAGCTCAGA ACACCGCTGG CATGAAAGCA TTGACACAAA CACATCTTCT 
ACCCCCGCCA TCACCTTCCT CATTCAAGAG GCTCTGATCT CCGGAGGCCT CGCTGCTCTG 
GCCCGCGACC CATCCTTTGT TGCAGTGACC AGGGATGAGA TGGCAGCCAG CAGCCAGATG 
GAGATGGACG AAGTGGAGAA AGCTGCAGTG GAGCTGCTAC AGGGAAGAGA AGCACTGCAG 
GACACAGAAA TCAGCTCTGC TCCCCGGGAC ACCTTGGCTG CATCAGCAGT CAGCACCCCG 
GGGCTCAGCG TGGCCGCATC CCATCAGACC AGCTCTGCTG CTCGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292076.1
CDS	130..5640
Translation	MEPASHQDDVKKRQQKEKSKKPVPPVAPRPPRALFCLTLQNPLRKACISIVEWKPFEIIILLTIFANCVALAIYQPMPEDDTNVANSSLEKLEYVFLIFFAIEAMLKIIAYGFLFHTDAYLRNGWNVLDFSIVSLGLVTMTLEQINAKEGGSLGGKGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGQELFKGKMHKTCYYLGTDVIATVGSEKPAPCTTSGHGRHCSINGTECRGGWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDMKGYMDWITHAEVMDSDRARGEGMMPLDEGGSETESLYEIEGMNKWILYFRQWRRWNRMFRRKCRDVVKSKFFYWLVILLVALNTLSIASEHHFQPEWLTIVQDNANRVLLALFVAEMLLKMYALGLRQYFMSLFNRFDCFVVCAGVLEIILVELSTLSPLGISVLRCIRLLRIFKITRYWTSLSNLVASLLNSVRSIASLLLLLFLFIIVFALLGMQLFGGMYDFEDMEVRRSTFDNFPQALISVFQILTGEDWNSIMYNGIMAYGGPSFPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGYPEKSEDEKQMLAKKLEQKAKGEGIPTTAKLKVDEFESNVNEIKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKFRMLCHRIVNATWFTNFILLFILLSSISLAAEDPIRAESFRNQILGYFDIGFTSVFTVEIVLKMTAYGAFLHKGSFCRNSFNILDLLVVAVSLISMGFESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVVVTTLLQFMFACIGVQLFKGKFYSCTDPSKLTEKECRGHFINYVDGDPTQIELKERVWFHNAFHFNNVLSAMMSLFTVSTFEGWPELLYRAIDTNDENKGPIYNYRVEIAMFFIIYIILIAFFMMNIFVGFVIVTFQEQGESEYKNCELDKNQRQCVQYALKARPLRRYIPKNPYQYQIWYVVTSSYFEYLMFFLIMLNTICLGMQHYNQSAEMNHVSDILNVAFTVLFTLEMILKLMAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDTVLASSGGLYCLGGGCDSIDPDDNSRVSITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLDCSYGKRCDPESDYAEGEEYTCGTGFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVCMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRTIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKNPVEIQAGLRSIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIYRRTGGLFGQVDSFLEPSSPLQPHVASQRPLQFTEVGSEDLDSPVFLDDFPQEGNTNVNNANWQQGLEYEDEVLRRAVPRPPVREPDLPIPLERLRRRRGAASEHHPASGPSLHPQQGSTVTAAASSSEHRWHESIDTNTSSTPAITFLIQEALISGGLAALARDPSFVAVTRDEMAASSQMEMDEVEKAAVELLQGREALQDTEISSAPRDTLAASAVSTPGLSVAASHQTSSAARL

Target ORF information:

RefSeq Version	NM_001305147.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305147.1

ORF Insert Sequence:

ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG 
AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC 
CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT 
GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT 
GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT 
CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG 
ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT 
GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA 
AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA 
CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG 
AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG 
TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT 
GCCATGCTGA CTGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG 
GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT 
GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG 
CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG 
GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT 
GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA 
ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC 
CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG 
CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG 
CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC 
GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG 
TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC 
AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT 
GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTACT GGGCATGCAG 
CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC 
TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA 
ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC 
TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT 
GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG 
GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG 
ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG 
TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA 
GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT 
CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC 
TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT 
TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG 
GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC 
ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC 
AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT 
CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG 
AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG 
TGTGTGTTCG TGGCCATCAA GACCATTGGT AACATTGTGG TCGTTACGAC GTTGCTGCAG 
TTCATGTTTG CCTGCATTGG GGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT 
CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC 
CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT 
GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG 
CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG 
GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC 
TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT 
GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG 
CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC 
TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT 
TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC 
CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG 
GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC 
AGCGAGATTG ATACTGTTCT TGCATCCAGT GGTGGATTGT ACTGCCTCGG CGGAGGTTGT 
GATAGCATTG ACCCGGATGA CAACTCCCGT GTCTCCATCA CGTTCTTCCG GCTGTTCCGA 
GTGATGCGGC TGGTGAAGCT GCTGAGCCGG GGAGAAGGCG TCAGGACCCT CCTGTGGACC 
TTCATCAAAT CCTTCCAGGC TCTGCCCTAT GTTGCCCTGC TGATCGTGAT GCTGTTCTTC 
ATCTATGCTG TGATCGGGAT GCAGATGTTT GGGAAGATTG CCATGGTGGA CGGAACCCAG 
ATCAACCGAA ACAACAACTT CCAAACCTTC CCACAAGCTG TGCTGCTGCT CTTCAGGTGT 
GCCACGGGGG AGGCCTGGCA GGAGATCCTG CTGGACTGCA GCTATGGGAA GCGGTGCGAC 
CCTGAGTCTG ACTACGCGGA GGGCGAGGAG TACACCTGTG GCACGGGCTT TGCCTACTTC 
TATTTCATCA GCTTCTACAT GCTCTGTGCC TTTCTGATCA TTAACCTTTT TGTGGCTGTC 
ATCATGGACA ACTTTGACTA CCTCACACGT GACTGGTCCA TCCTGGGGCC CCACCACCTG 
GATGAGTTCA AACGGATCTG GGCTGAGTAC GACCCTGAAG CCAAGGGCAG GATTAAGCAC 
TTGGATGTGG TGACCCTGCT GAGACGTATC CAACCTCCCC TGGGCTTTGG GAAGTTCTGC 
CCTCACCGTG TAGCTTGTAA GCGCCTGGTG TGCATGAACA TGCCCCTGAA CAGCGATGGC 
ACTGTCACCT TCAATGCAAC CCTCTTTGCG CTGGTGAGGA CAGCCCTCAA AATCAAGACA 
GAAGGTAACT TTGAACAGGC CAATGAGGAG CTCAGAACCA TTATCAAAAA AATCTGGAAG 
CGAACCAGTA TGAAGCTTTT GGACCAGGTC ATCCCACCTA TTGGAGATGA TGAGGTGACA 
GTGGGCAAAT TCTATGCTAC TTTCCTCATC CAAGAGCATT TCCGAAAGTT CATGAAGCGC 
CAGGAGGAGT ACTATGGGTA CCGTCCCAAG AAGAACCCTG TAGAGATCCA GGCTGGGCTG 
AGAAGCATTG AAGAAGAAGC TGCTCCTGAA ATTCATCGAG CCATCTCTGG GGACCTGACT 
GCTGAGGAGG AGCTGGAGAG GGCAATGGTG GAAGCTGCCA TGGAGGAGGG GATATACAGG 
AGGACGGGAG GTTTGTTTGG CCAGGTGGAC AGCTTCCTGG AGCCGAGCAG CCCCCTGCAG 
CCCCACGTGG CCAGCCAGAG GCCCCTGCAG TTCACAGAGG TGGGCAGCGA AGACCTCGAT 
TCCCCCGTCT TCCTCGACGA CTTCCCCCAG GAAGGCAACA CCAATGTCAA CAACGCCAAC 
TGGCAGCAGG GGTTGGAATA CGAGGATGAG GTGCTGAGGA GAGCAGTGCC CAGGCCTCCA 
GTGCGGGAGC CTGACCTCCC CATCCCTTTG GAGAGGCTGC GGCGGAGGCG AGGAGCTGCC 
TCTGAGCATC ACCCAGCATC GGGGCCATCC CTCCATCCCC AGCAGGGCAG CACAGTGACA 
GCAGCAGCAT CCAGCTCAGA ACACCGCTGG CATGAAAGCA TTGACACAAA CACATCTTCT 
ACCCCCGCCA TCACCTTCCT CATTCAAGAG GCTCTGATCT CCGGAGGCCT CGCTGCTCTG 
GCCCGCGACC CATCCTTTGT TGCAGTGACC AGGGATGAGA TGGCAGCCAG CAGCCAGATG 
GAGATGGACG AAGTGGAGAA AGCTGCAGTG GAGCTGCTAC AGGGAAGAGA AGCACTGCAG 
GACACAGAAA TCAGCTCTGC TCCCCGGGAC ACCTTGGCTG CATCAGCAGT CAGCACCCCG 
GGGCTCAGCG TGGCCGCATC CCATCAGACC AGCTCTGCTG CTCGCCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1S ( NM_001305148.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001292077.1 Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 2, mRNA.

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CloneID	OGa19561
Clone ID Related Accession (Same CDS sequence)	NM_001305148.1
Accession Version	NM_001305148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-11
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1S isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000168.1. On Feb 25, 2015 this sequence version replaced XM_004934808.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on protein alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201367, SAMEA2201369 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG 
AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC 
CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT 
GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT 
GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT 
CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG 
ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT 
GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA 
AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA 
CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG 
AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG 
TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT 
GCCATGCTGA CTGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG 
GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT 
GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG 
CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG 
GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT 
GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA 
ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC 
CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG 
CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG 
CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC 
GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG 
TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC 
AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT 
GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTACT GGGCATGCAG 
CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC 
TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA 
ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC 
TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT 
GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG 
GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG 
ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG 
TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA 
GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT 
CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC 
TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT 
TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG 
GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC 
ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC 
AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT 
CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG 
AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG 
TGTGTGTTCG TGGCCATCAA GACCATTGGT AACATTGTGG TCGTTACGAC GTTGCTGCAG 
TTCATGTTTG CCTGCATTGG GGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT 
CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC 
CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT 
GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG 
CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG 
GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC 
TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT 
GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG 
CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC 
TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT 
TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC 
CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG 
GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC 
AGCGAGATTG ATGACCCGGA TGACAACTCC CGTGTCTCCA TCACGTTCTT CCGGCTGTTC 
CGAGTGATGC GGCTGGTGAA GCTGCTGAGC CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG 
ACCTTCATCA AATCCTTCCA GGCTCTGCCC TATGTTGCCC TGCTGATCGT GATGCTGTTC 
TTCATCTATG CTGTGATCGG GATGCAGATG TTTGGGAAGA TTGCCATGGT GGACGGAACC 
CAGATCAACC GAAACAACAA CTTCCAAACC TTCCCACAAG CTGTGCTGCT GCTCTTCAGG 
TGTGCCACGG GGGAGGCCTG GCAGGAGATC CTGCTGGACT GCAGCTATGG GAAGCGGTGC 
GACCCTGAGT CTGACTACGC GGAGGGCGAG GAGTACACCT GTGGCACGGG CTTTGCCTAC 
TTCTATTTCA TCAGCTTCTA CATGCTCTGT GCCTTTCTGA TCATTAACCT TTTTGTGGCT 
GTCATCATGG ACAACTTTGA CTACCTCACA CGTGACTGGT CCATCCTGGG GCCCCACCAC 
CTGGATGAGT TCAAACGGAT CTGGGCTGAG TACGACCCTG AAGCCAAGGG CAGGATTAAG 
CACTTGGATG TGGTGACCCT GCTGAGACGT ATCCAACCTC CCCTGGGCTT TGGGAAGTTC 
TGCCCTCACC GTGTAGCTTG TAAGCGCCTG GTGTGCATGA ACATGCCCCT GAACAGCGAT 
GGCACTGTCA CCTTCAATGC AACCCTCTTT GCGCTGGTGA GGACAGCCCT CAAAATCAAG 
ACAGAAGGTA ACTTTGAACA GGCCAATGAG GAGCTCAGAA CCATTATCAA AAAAATCTGG 
AAGCGAACCA GTATGAAGCT TTTGGACCAG GTCATCCCAC CTATTGGAGA TGATGAGGTG 
ACAGTGGGCA AATTCTATGC TACTTTCCTC ATCCAAGAGC ATTTCCGAAA GTTCATGAAG 
CGCCAGGAGG AGTACTATGG GTACCGTCCC AAGAAGAACC CTGTAGAGAT CCAGGCTGGG 
CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT GAAATTCATC GAGCCATCTC TGGGGACCTG 
ACTGCTGAGG AGGAGCTGGA GAGGGCAATG GTGGAAGCTG CCATGGAGGA GGGGATATAC 
AGGAGGACGG GAGGTTTGTT TGGCCAGGTG GACAGCTTCC TGGAGCCGAG CAGCCCCCTG 
CAGCCCCACG TGGCCAGCCA GAGGCCCCTG CAGTTCACAG AGGTGGGCAG CGAAGACCTC 
GATTCCCCCG TCTTCCTCGA CGACTTCCCC CAGGAAGGCA ACACCAATGT CAACAACGCC 
AACTGGCAGC AGGGGTTGGA ATACGAGGAT GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT 
CCAGTGCGGG AGCCTGACCT CCCCATCCCT TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT 
GCCTCTGAGC ATCACCCAGC ATCGGGGCCA TCCCTCCATC CCCAGCAGGG CAGCACAGTG 
ACAGCAGCAG CATCCAGCTC AGAACACCGC TGGCATGAAA GCATTGACAC AAACACATCT 
TCTACCCCCG CCATCACCTT CCTCATTCAA GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT 
CTGGCCCGCG ACCCATCCTT TGTTGCAGTG ACCAGGGATG AGATGGCAGC CAGCAGCCAG 
ATGGAGATGG ACGAAGTGGA GAAAGCTGCA GTGGAGCTGC TACAGGGAAG AGAAGCACTG 
CAGGACACAG AAATCAGCTC TGCTCCCCGG GACACCTTGG CTGCATCAGC AGTCAGCACC 
CCGGGGCTCA GCGTGGCCGC ATCCCATCAG ACCAGCTCTG CTGCTCGCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292077.1
CDS	130..5583
Translation	MEPASHQDDVKKRQQKEKSKKPVPPVAPRPPRALFCLTLQNPLRKACISIVEWKPFEIIILLTIFANCVALAIYQPMPEDDTNVANSSLEKLEYVFLIFFAIEAMLKIIAYGFLFHTDAYLRNGWNVLDFSIVSLGLVTMTLEQINAKEGGSLGGKGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGQELFKGKMHKTCYYLGTDVIATVGSEKPAPCTTSGHGRHCSINGTECRGGWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDMKGYMDWITHAEVMDSDRARGEGMMPLDEGGSETESLYEIEGMNKWILYFRQWRRWNRMFRRKCRDVVKSKFFYWLVILLVALNTLSIASEHHFQPEWLTIVQDNANRVLLALFVAEMLLKMYALGLRQYFMSLFNRFDCFVVCAGVLEIILVELSTLSPLGISVLRCIRLLRIFKITRYWTSLSNLVASLLNSVRSIASLLLLLFLFIIVFALLGMQLFGGMYDFEDMEVRRSTFDNFPQALISVFQILTGEDWNSIMYNGIMAYGGPSFPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGYPEKSEDEKQMLAKKLEQKAKGEGIPTTAKLKVDEFESNVNEIKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPMPEASSFFIFSPTNKFRMLCHRIVNATWFTNFILLFILLSSISLAAEDPIRAESFRNQILGYFDIGFTSVFTVEIVLKMTAYGAFLHKGSFCRNSFNILDLLVVAVSLISMGFESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVVVTTLLQFMFACIGVQLFKGKFYSCTDPSKLTEKECRGHFINYVDGDPTQIELKERVWFHNAFHFNNVLSAMMSLFTVSTFEGWPELLYRAIDTNDENKGPIYNYRVEIAMFFIIYIILIAFFMMNIFVGFVIVTFQEQGESEYKNCELDKNQRQCVQYALKARPLRRYIPKNPYQYQIWYVVTSSYFEYLMFFLIMLNTICLGMQHYNQSAEMNHVSDILNVAFTVLFTLEMILKLMAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDDPDDNSRVSITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLDCSYGKRCDPESDYAEGEEYTCGTGFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVCMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRTIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKNPVEIQAGLRSIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIYRRTGGLFGQVDSFLEPSSPLQPHVASQRPLQFTEVGSEDLDSPVFLDDFPQEGNTNVNNANWQQGLEYEDEVLRRAVPRPPVREPDLPIPLERLRRRRGAASEHHPASGPSLHPQQGSTVTAAASSSEHRWHESIDTNTSSTPAITFLIQEALISGGLAALARDPSFVAVTRDEMAASSQMEMDEVEKAAVELLQGREALQDTEISSAPRDTLAASAVSTPGLSVAASHQTSSAARL

Target ORF information:

RefSeq Version	NM_001305148.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305148.1

ORF Insert Sequence:

ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG 
AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC 
CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT 
GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT 
GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT 
CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG 
ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT 
GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA 
AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA 
CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG 
AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG 
TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT 
GCCATGCTGA CTGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG 
GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT 
GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG 
CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG 
GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT 
GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA 
ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC 
CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG 
CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG 
CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC 
GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG 
TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC 
AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT 
GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTACT GGGCATGCAG 
CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC 
TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA 
ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC 
TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT 
GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG 
GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG 
ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG 
TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA 
GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT 
CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC 
TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT 
TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG 
GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC 
ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC 
AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT 
CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG 
AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG 
TGTGTGTTCG TGGCCATCAA GACCATTGGT AACATTGTGG TCGTTACGAC GTTGCTGCAG 
TTCATGTTTG CCTGCATTGG GGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT 
CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC 
CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT 
GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG 
CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG 
GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC 
TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT 
GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG 
CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC 
TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT 
TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC 
CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG 
GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC 
AGCGAGATTG ATGACCCGGA TGACAACTCC CGTGTCTCCA TCACGTTCTT CCGGCTGTTC 
CGAGTGATGC GGCTGGTGAA GCTGCTGAGC CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG 
ACCTTCATCA AATCCTTCCA GGCTCTGCCC TATGTTGCCC TGCTGATCGT GATGCTGTTC 
TTCATCTATG CTGTGATCGG GATGCAGATG TTTGGGAAGA TTGCCATGGT GGACGGAACC 
CAGATCAACC GAAACAACAA CTTCCAAACC TTCCCACAAG CTGTGCTGCT GCTCTTCAGG 
TGTGCCACGG GGGAGGCCTG GCAGGAGATC CTGCTGGACT GCAGCTATGG GAAGCGGTGC 
GACCCTGAGT CTGACTACGC GGAGGGCGAG GAGTACACCT GTGGCACGGG CTTTGCCTAC 
TTCTATTTCA TCAGCTTCTA CATGCTCTGT GCCTTTCTGA TCATTAACCT TTTTGTGGCT 
GTCATCATGG ACAACTTTGA CTACCTCACA CGTGACTGGT CCATCCTGGG GCCCCACCAC 
CTGGATGAGT TCAAACGGAT CTGGGCTGAG TACGACCCTG AAGCCAAGGG CAGGATTAAG 
CACTTGGATG TGGTGACCCT GCTGAGACGT ATCCAACCTC CCCTGGGCTT TGGGAAGTTC 
TGCCCTCACC GTGTAGCTTG TAAGCGCCTG GTGTGCATGA ACATGCCCCT GAACAGCGAT 
GGCACTGTCA CCTTCAATGC AACCCTCTTT GCGCTGGTGA GGACAGCCCT CAAAATCAAG 
ACAGAAGGTA ACTTTGAACA GGCCAATGAG GAGCTCAGAA CCATTATCAA AAAAATCTGG 
AAGCGAACCA GTATGAAGCT TTTGGACCAG GTCATCCCAC CTATTGGAGA TGATGAGGTG 
ACAGTGGGCA AATTCTATGC TACTTTCCTC ATCCAAGAGC ATTTCCGAAA GTTCATGAAG 
CGCCAGGAGG AGTACTATGG GTACCGTCCC AAGAAGAACC CTGTAGAGAT CCAGGCTGGG 
CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT GAAATTCATC GAGCCATCTC TGGGGACCTG 
ACTGCTGAGG AGGAGCTGGA GAGGGCAATG GTGGAAGCTG CCATGGAGGA GGGGATATAC 
AGGAGGACGG GAGGTTTGTT TGGCCAGGTG GACAGCTTCC TGGAGCCGAG CAGCCCCCTG 
CAGCCCCACG TGGCCAGCCA GAGGCCCCTG CAGTTCACAG AGGTGGGCAG CGAAGACCTC 
GATTCCCCCG TCTTCCTCGA CGACTTCCCC CAGGAAGGCA ACACCAATGT CAACAACGCC 
AACTGGCAGC AGGGGTTGGA ATACGAGGAT GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT 
CCAGTGCGGG AGCCTGACCT CCCCATCCCT TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT 
GCCTCTGAGC ATCACCCAGC ATCGGGGCCA TCCCTCCATC CCCAGCAGGG CAGCACAGTG 
ACAGCAGCAG CATCCAGCTC AGAACACCGC TGGCATGAAA GCATTGACAC AAACACATCT 
TCTACCCCCG CCATCACCTT CCTCATTCAA GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT 
CTGGCCCGCG ACCCATCCTT TGTTGCAGTG ACCAGGGATG AGATGGCAGC CAGCAGCCAG 
ATGGAGATGG ACGAAGTGGA GAAAGCTGCA GTGGAGCTGC TACAGGGAAG AGAAGCACTG 
CAGGACACAG AAATCAGCTC TGCTCCCCGG GACACCTTGG CTGCATCAGC AGTCAGCACC 
CCGGGGCTCA GCGTGGCCGC ATCCCATCAG ACCAGCTCTG CTGCTCGCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

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CACNA1S cDNA ORF clone, Gallus gallus(chicken)

Summary

Reference Sequences (RefSeq)

Pathways from BioSystems

Homologies

Gene Ontology

Bibliography

Reference Sequences (Refseq)

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

PubMed

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