CACNA1S cDNA ORF clone, Gallus gallus(chicken)

  • Gene

  • Clones

  • gRNAs

The following CACNA1S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa147930 XM_025143672.1
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa147930 XM_025143673.1
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa147931 XM_025143674.1
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa147932 XM_025143675.1
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa147933 XM_015298692.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa46362 XM_015298692.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa19560 NM_001305147.1
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa19561 NM_001305148.1
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa147930
    Clone ID Related Accession (Same CDS sequence) XM_025143672.1 , XM_025143673.1
    Accession Version XM_025143672.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC
    AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC
    CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC
    CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC
    TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG
    TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC
    TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC
    CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG
    GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT
    GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA
    CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG
    TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT
    ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG
    GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT
    GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG
    GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG
    GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC
    ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC
    AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT
    GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG
    GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG
    GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG
    CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC
    CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGGTTGGA ATACGAGGAT
    GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT CCAGTGCGGG AGCCTGACCT CCCCATCCCT
    TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT GCCTCTGAGC ATCACCCAGC ATCGGGGCCA
    TCCCTCCATC CCCAGCAGGG CAGCACAGTG ACAGCAGCAG CATCCAGCTC AGAACACCGC
    TGGCATGAAA GCATTGACAC AAACACATCT TCTACCCCCG CCATCACCTT CCTCATTCAA
    GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT CTGGCCCGCG ACCCATCCTT TGTTGCAGTG
    ACCAGGGATG AGATGGCAGC CAGCAGCCAG ATGGAGATGG ACGAAGTGGA GAAAGCTGCA
    GTGGAGCTGC TACAGGGAAG AGAAGCACTG CAGGACACAG AAATCAGCTC TGCTCCCCGG
    GACACCTTGG CTGCATCAGC AGTCAGCACC CCGGGGCTCA GCGTGGCCGC ATCCCATCAG
    ACCAGCTCTG CTGCTCGCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024999440.1
    CDS1641..7304
    Translation

    Target ORF information:

    RefSeq Version XM_025143672.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025143672.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC
    AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC
    CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC
    CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC
    TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG
    TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC
    TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC
    CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG
    GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT
    GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA
    CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG
    TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT
    ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG
    GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT
    GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG
    GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG
    GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC
    ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC
    AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT
    GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG
    GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG
    GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG
    CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC
    CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGGTTGGA ATACGAGGAT
    GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT CCAGTGCGGG AGCCTGACCT CCCCATCCCT
    TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT GCCTCTGAGC ATCACCCAGC ATCGGGGCCA
    TCCCTCCATC CCCAGCAGGG CAGCACAGTG ACAGCAGCAG CATCCAGCTC AGAACACCGC
    TGGCATGAAA GCATTGACAC AAACACATCT TCTACCCCCG CCATCACCTT CCTCATTCAA
    GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT CTGGCCCGCG ACCCATCCTT TGTTGCAGTG
    ACCAGGGATG AGATGGCAGC CAGCAGCCAG ATGGAGATGG ACGAAGTGGA GAAAGCTGCA
    GTGGAGCTGC TACAGGGAAG AGAAGCACTG CAGGACACAG AAATCAGCTC TGCTCCCCGG
    GACACCTTGG CTGCATCAGC AGTCAGCACC CCGGGGCTCA GCGTGGCCGC ATCCCATCAG
    ACCAGCTCTG CTGCTCGCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa147930
    Clone ID Related Accession (Same CDS sequence) XM_025143672.1 , XM_025143673.1
    Accession Version XM_025143673.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC
    AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC
    CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC
    CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC
    TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG
    TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC
    TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC
    CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG
    GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT
    GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA
    CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG
    TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT
    ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG
    GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT
    GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG
    GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG
    GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC
    ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC
    AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT
    GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG
    GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG
    GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG
    CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC
    CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGGTTGGA ATACGAGGAT
    GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT CCAGTGCGGG AGCCTGACCT CCCCATCCCT
    TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT GCCTCTGAGC ATCACCCAGC ATCGGGGCCA
    TCCCTCCATC CCCAGCAGGG CAGCACAGTG ACAGCAGCAG CATCCAGCTC AGAACACCGC
    TGGCATGAAA GCATTGACAC AAACACATCT TCTACCCCCG CCATCACCTT CCTCATTCAA
    GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT CTGGCCCGCG ACCCATCCTT TGTTGCAGTG
    ACCAGGGATG AGATGGCAGC CAGCAGCCAG ATGGAGATGG ACGAAGTGGA GAAAGCTGCA
    GTGGAGCTGC TACAGGGAAG AGAAGCACTG CAGGACACAG AAATCAGCTC TGCTCCCCGG
    GACACCTTGG CTGCATCAGC AGTCAGCACC CCGGGGCTCA GCGTGGCCGC ATCCCATCAG
    ACCAGCTCTG CTGCTCGCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024999441.1
    CDS723..6386
    Translation

    Target ORF information:

    RefSeq Version XM_025143673.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025143673.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC
    AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC
    CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC
    CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC
    TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG
    TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC
    TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC
    CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG
    GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT
    GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA
    CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG
    TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT
    ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG
    GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT
    GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG
    GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG
    GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC
    ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC
    AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT
    GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG
    GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG
    GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG
    CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC
    CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGGTTGGA ATACGAGGAT
    GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT CCAGTGCGGG AGCCTGACCT CCCCATCCCT
    TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT GCCTCTGAGC ATCACCCAGC ATCGGGGCCA
    TCCCTCCATC CCCAGCAGGG CAGCACAGTG ACAGCAGCAG CATCCAGCTC AGAACACCGC
    TGGCATGAAA GCATTGACAC AAACACATCT TCTACCCCCG CCATCACCTT CCTCATTCAA
    GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT CTGGCCCGCG ACCCATCCTT TGTTGCAGTG
    ACCAGGGATG AGATGGCAGC CAGCAGCCAG ATGGAGATGG ACGAAGTGGA GAAAGCTGCA
    GTGGAGCTGC TACAGGGAAG AGAAGCACTG CAGGACACAG AAATCAGCTC TGCTCCCCGG
    GACACCTTGG CTGCATCAGC AGTCAGCACC CCGGGGCTCA GCGTGGCCGC ATCCCATCAG
    ACCAGCTCTG CTGCTCGCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa147931
    Clone ID Related Accession (Same CDS sequence) XM_025143674.1
    Accession Version XM_025143674.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5607bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATGACCC GGATGACAAC
    TCCCGTGTCT CCATCACGTT CTTCCGGCTG TTCCGAGTGA TGCGGCTGGT GAAGCTGCTG
    AGCCGGGGAG AAGGCGTCAG GACCCTCCTG TGGACCTTCA TCAAATCCTT CCAGGCTCTG
    CCCTATGTTG CCCTGCTGAT CGTGATGCTG TTCTTCATCT ATGCTGTGAT CGGGATGCAG
    ATGTTTGGGA AGATTGCCAT GGTGGACGGA ACCCAGATCA ACCGAAACAA CAACTTCCAA
    ACCTTCCCAC AAGCTGTGCT GCTGCTCTTC AGGTGTGCCA CGGGGGAGGC CTGGCAGGAG
    ATCCTGCTGG ACTGCAGCTA TGGGAAGCGG TGCGACCCTG AGTCTGACTA CGCGGAGGGC
    GAGGAGTACA CCTGTGGCAC GGGCTTTGCC TACTTCTATT TCATCAGCTT CTACATGCTC
    TGTGCCTTTC TGATCATTAA CCTTTTTGTG GCTGTCATCA TGGACAACTT TGACTACCTC
    ACACGTGACT GGTCCATCCT GGGGCCCCAC CACCTGGATG AGTTCAAACG GATCTGGGCT
    GAGTACGACC CTGAAGCCAA GGGCAGGATT AAGCACTTGG ATGTGGTGAC CCTGCTGAGA
    CGTATCCAAC CTCCCCTGGG CTTTGGGAAG TTCTGCCCTC ACCGTGTAGC TTGTAAGCGC
    CTGGTGTGCA TGAACATGCC CCTGAACAGC GATGGCACTG TCACCTTCAA TGCAACCCTC
    TTTGCGCTGG TGAGGACAGC CCTCAAAATC AAGACAGAAG GTAACTTTGA ACAGGCCAAT
    GAGGAGCTCA GAACCATTAT CAAAAAAATC TGGAAGCGAA CCAGTATGAA GCTTTTGGAC
    CAGGTCATCC CACCTATTGG AGATGATGAG GTGACAGTGG GCAAATTCTA TGCTACTTTC
    CTCATCCAAG AGCATTTCCG AAAGTTCATG AAGCGCCAGG AGGAGTACTA TGGGTACCGT
    CCCAAGAAGA ACCCTGTAGA GATCCAGGCT GGGCTGAGAA GCATTGAAGA AGAAGCTGCT
    CCTGAAATTC ATCGAGCCAT CTCTGGGGAC CTGACTGCTG AGGAGGAGCT GGAGAGGGCA
    ATGGTGGAAG CTGCCATGGA GGAGGGGATA TACAGGAGGA CGGGAGGTTT GTTTGGCCAG
    GTGGACAGCT TCCTGGAGCC GAGCAGCCCC CTGCAGCCCC ACGTGGCCAG CCAGAGGCCC
    CTGCAGTTCA CAGAGGTGGG CAGCGAAGAC CTCGATTCCC CCGTCTTCCT CGACGACTTC
    CCCCAGGAAG GCAACACCAA TGTCAACAAC GCCAACTGGC AGCAGGGGTT GGAATACGAG
    GATGAGGTGC TGAGGAGAGC AGTGCCCAGG CCTCCAGTGC GGGAGCCTGA CCTCCCCATC
    CCTTTGGAGA GGCTGCGGCG GAGGCGAGGA GCTGCCTCTG AGCATCACCC AGCATCGGGG
    CCATCCCTCC ATCCCCAGCA GGGCAGCACA GTGACAGCAG CAGCATCCAG CTCAGAACAC
    CGCTGGCATG AAAGCATTGA CACAAACACA TCTTCTACCC CCGCCATCAC CTTCCTCATT
    CAAGAGGCTC TGATCTCCGG AGGCCTCGCT GCTCTGGCCC GCGACCCATC CTTTGTTGCA
    GTGACCAGGG ATGAGATGGC AGCCAGCAGC CAGATGGAGA TGGACGAAGT GGAGAAAGCT
    GCAGTGGAGC TGCTACAGGG AAGAGAAGCA CTGCAGGACA CAGAAATCAG CTCTGCTCCC
    CGGGACACCT TGGCTGCATC AGCAGTCAGC ACCCCGGGGC TCAGCGTGGC CGCATCCCAT
    CAGACCAGCT CTGCTGCTCG CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024999442.1
    CDS1641..7247
    Translation

    Target ORF information:

    RefSeq Version XM_025143674.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025143674.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATGACCC GGATGACAAC
    TCCCGTGTCT CCATCACGTT CTTCCGGCTG TTCCGAGTGA TGCGGCTGGT GAAGCTGCTG
    AGCCGGGGAG AAGGCGTCAG GACCCTCCTG TGGACCTTCA TCAAATCCTT CCAGGCTCTG
    CCCTATGTTG CCCTGCTGAT CGTGATGCTG TTCTTCATCT ATGCTGTGAT CGGGATGCAG
    ATGTTTGGGA AGATTGCCAT GGTGGACGGA ACCCAGATCA ACCGAAACAA CAACTTCCAA
    ACCTTCCCAC AAGCTGTGCT GCTGCTCTTC AGGTGTGCCA CGGGGGAGGC CTGGCAGGAG
    ATCCTGCTGG ACTGCAGCTA TGGGAAGCGG TGCGACCCTG AGTCTGACTA CGCGGAGGGC
    GAGGAGTACA CCTGTGGCAC GGGCTTTGCC TACTTCTATT TCATCAGCTT CTACATGCTC
    TGTGCCTTTC TGATCATTAA CCTTTTTGTG GCTGTCATCA TGGACAACTT TGACTACCTC
    ACACGTGACT GGTCCATCCT GGGGCCCCAC CACCTGGATG AGTTCAAACG GATCTGGGCT
    GAGTACGACC CTGAAGCCAA GGGCAGGATT AAGCACTTGG ATGTGGTGAC CCTGCTGAGA
    CGTATCCAAC CTCCCCTGGG CTTTGGGAAG TTCTGCCCTC ACCGTGTAGC TTGTAAGCGC
    CTGGTGTGCA TGAACATGCC CCTGAACAGC GATGGCACTG TCACCTTCAA TGCAACCCTC
    TTTGCGCTGG TGAGGACAGC CCTCAAAATC AAGACAGAAG GTAACTTTGA ACAGGCCAAT
    GAGGAGCTCA GAACCATTAT CAAAAAAATC TGGAAGCGAA CCAGTATGAA GCTTTTGGAC
    CAGGTCATCC CACCTATTGG AGATGATGAG GTGACAGTGG GCAAATTCTA TGCTACTTTC
    CTCATCCAAG AGCATTTCCG AAAGTTCATG AAGCGCCAGG AGGAGTACTA TGGGTACCGT
    CCCAAGAAGA ACCCTGTAGA GATCCAGGCT GGGCTGAGAA GCATTGAAGA AGAAGCTGCT
    CCTGAAATTC ATCGAGCCAT CTCTGGGGAC CTGACTGCTG AGGAGGAGCT GGAGAGGGCA
    ATGGTGGAAG CTGCCATGGA GGAGGGGATA TACAGGAGGA CGGGAGGTTT GTTTGGCCAG
    GTGGACAGCT TCCTGGAGCC GAGCAGCCCC CTGCAGCCCC ACGTGGCCAG CCAGAGGCCC
    CTGCAGTTCA CAGAGGTGGG CAGCGAAGAC CTCGATTCCC CCGTCTTCCT CGACGACTTC
    CCCCAGGAAG GCAACACCAA TGTCAACAAC GCCAACTGGC AGCAGGGGTT GGAATACGAG
    GATGAGGTGC TGAGGAGAGC AGTGCCCAGG CCTCCAGTGC GGGAGCCTGA CCTCCCCATC
    CCTTTGGAGA GGCTGCGGCG GAGGCGAGGA GCTGCCTCTG AGCATCACCC AGCATCGGGG
    CCATCCCTCC ATCCCCAGCA GGGCAGCACA GTGACAGCAG CAGCATCCAG CTCAGAACAC
    CGCTGGCATG AAAGCATTGA CACAAACACA TCTTCTACCC CCGCCATCAC CTTCCTCATT
    CAAGAGGCTC TGATCTCCGG AGGCCTCGCT GCTCTGGCCC GCGACCCATC CTTTGTTGCA
    GTGACCAGGG ATGAGATGGC AGCCAGCAGC CAGATGGAGA TGGACGAAGT GGAGAAAGCT
    GCAGTGGAGC TGCTACAGGG AAGAGAAGCA CTGCAGGACA CAGAAATCAG CTCTGCTCCC
    CGGGACACCT TGGCTGCATC AGCAGTCAGC ACCCCGGGGC TCAGCGTGGC CGCATCCCAT
    CAGACCAGCT CTGCTGCTCG CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa147932
    Clone ID Related Accession (Same CDS sequence) XM_025143675.1
    Accession Version XM_025143675.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC
    AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC
    CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC
    CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC
    TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG
    TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC
    TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC
    CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG
    GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT
    GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA
    CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG
    TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT
    ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG
    GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT
    GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG
    GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG
    GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC
    ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC
    AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT
    GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG
    GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG
    GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG
    CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC
    CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGAGTGGA GAAGATGGGG
    AGGGCTGCAT CCTGGGCTCA AAGTGGTGGT CCCTGCTCGG CTGACCCCTC TGCCTGCCCC
    TGCCAGGTTG GAATACGAGG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024999443.1
    CDS1641..6884
    Translation

    Target ORF information:

    RefSeq Version XM_025143675.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025143675.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC
    AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC
    CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC
    CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC
    TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG
    TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC
    TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC
    CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG
    GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT
    GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA
    CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG
    TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT
    ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG
    GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT
    GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG
    GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG
    GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC
    ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC
    AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT
    GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG
    GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG
    GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG
    CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC
    CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGAGTGGA GAAGATGGGG
    AGGGCTGCAT CCTGGGCTCA AAGTGGTGGT CCCTGCTCGG CTGACCCCTC TGCCTGCCCC
    TGCCAGGTTG GAATACGAGG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa147933
    Clone ID Related Accession (Same CDS sequence) XM_015298692.2
    Accession Version XM_015298692.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006113.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC
    AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC
    CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC
    CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC
    TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG
    TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC
    TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC
    CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG
    GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT
    GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA
    CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG
    TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT
    ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG
    GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT
    GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG
    GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG
    GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC
    ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC
    AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT
    GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG
    GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG
    GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG
    CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC
    CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGAGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154178.2
    CDS1641..6791
    Translation

    Target ORF information:

    RefSeq Version XM_015298692.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTGTCCTC TTGCTTGCCT GGCTTTCCTT ACTGGCTGGG GCACCGGAGA AAGAGGTGAC 
    AAGGGACAAA AGCACTTTTC CTTTGGAGAA GATCAGTGCG GTGCCAGGCA GCCGTCCCCA
    CCCCCAGCCC ACTCAGTGGC ATGTGAAGCA CTCGCCTGCC TCGCAATGTG CCGGGATGAT
    GTGAAGAAGA GGCAACAGAA GGAGAAATCC AAGAAGCCGG TGCCACCAGT AGCCCCTCGG
    CCACCCAGGG CTCTGTTCTG CTTAACCCTA CAGAACCCAC TGCGGAAGGC ATGCATCAGC
    ATCGTGGAGT GGAAACCTTT CGAGATCATC ATCCTTCTGA CCATCTTTGC CAACTGTGTT
    GCCCTGGCCA TCTACCAGCC CATGCCGGAG GATGACACCA ACGTGGCCAA CTCCAGCCTG
    GAGAAGTTGG AATATGTCTT CCTGATCTTC TTTGCCATCG AGGCCATGCT CAAGATAATA
    GCTTACGGGT TTCTGTTCCA TACGGACGCC TATCTCCGAA ACGGCTGGAA CGTCCTCGAC
    TTCTCCATCG TTTCCCTGGG GCTTGTCACC ATGACCCTGG AGCAGATCAA TGCAAAGGAG
    GGAGGGTCTT TGGGAGGGAA AGGTGGCTTT GATGTGAAGG CCCTGCGAGC GTTTCGTGTG
    CTGAGACCTC TGCGCCTGGT GTCCGGAGTG CCAAGTCTTC AGGTTGTCCT GAACTCCATT
    ATCAAAGCCA TGGTGCCTCT GCTCCACATT GCACTGCTCG TCCTCTTCAT GATCATCATC
    TATGCCATCG TGGGGCAAGA GCTCTTCAAG GGCAAGATGC ACAAGACCTG CTACTACCTT
    GGGACAGATG TGATCGCCAC GGTGGGGTCA GAGAAGCCAG CCCCCTGCAC AACCTCTGGC
    CATGGGCGTC ACTGCTCCAT CAATGGCACT GAGTGCCGTG GTGGCTGGCC GGGACCCAAC
    AATGGCATCA CTCACTTCGA TAACTTTGGC TTTGCCATGC TGACTGTCTA CCAGTGCATC
    ACCATGGAGG GCTGGACCGA AGTGCTCTAC TGGGTTAACG ATGCCATTGG AAATGAATGG
    CCATGGATCT ACTTTGTCAG CCTTATTTTG CTTGGTTCCT TCTTTGTTCT CAACCTGGTG
    CTGGGGGTGC TCAGTGGGGA GTTCACCAAA GAGCGTGAGA AGGCCAAGTC ACGAGGCACG
    TTTCAGAAGC TGCGGGAGAA GCAGCAGCTG GAGGAGGATA TGAAGGGCTA CATGGACTGG
    ATCACCCATG CTGAGGTGAT GGACAGTGAC CGGGCCAGAG GAGAAGGTAT GATGCCGTTG
    GATGAAGGAG GGTCAGAGAC AGAGAGCTTG TATGAAATTG AGGGCATGAA CAAATGGATT
    CTCTACTTCC GGCAGTGGAG GCGTTGGAAC CGAATGTTTC GTAGGAAGTG CAGGGATGTG
    GTGAAATCCA AGTTCTTCTA CTGGCTGGTC ATCCTGCTGG TGGCACTGAA TACCCTCTCC
    ATCGCCTCTG AGCACCATTT CCAGCCCGAA TGGCTGACGA TTGTACAAGA TAATGCCAAC
    CGGGTGCTGC TTGCTCTCTT TGTGGCCGAA ATGCTGCTGA AGATGTATGC ACTGGGCCTG
    CGCCAGTACT TCATGTCCCT CTTCAACCGC TTCGACTGCT TTGTTGTGTG TGCGGGGGTC
    CTGGAGATCA TCCTGGTGGA GCTCAGCACG TTGTCACCCC TGGGCATCTC TGTGCTGCGC
    TGCATCCGGC TGCTCCGCAT CTTCAAGATC ACCAGGTATT GGACATCACT GAGTAACCTG
    GTGGCCTCTC TGCTCAACTC GGTGCGCTCC ATTGCCTCTC TGCTCCTTCT CCTCTTCCTC
    TTCATCATTG TTTTTGCCCT ACTGGGCATG CAGCTCTTTG GGGGCATGTA TGACTTCGAG
    GACATGGAGG TGCGACGCAG CACATTCGAC AACTTCCCCC AGGCCCTCAT CAGTGTCTTC
    CAGATCCTGA CTGGAGAGGA CTGGAATTCA ATAATGTACA ATGGGATAAT GGCTTATGGG
    GGCCCATCCT TCCCTGGCAT GCTGGTCTGC ATCTACTTCA TCATCCTCTT TGTCTGTGGG
    AACTATATCC TCCTCAACGT CTTCCTGGCC ATTGCAGTTG ATAATCTGGC TGAGGCTGAG
    AGCCTGACCT CAGCTCAGAA GGCCAAAGCT GAGGAACGCA AGCGGCGGAA GATGTCCAGA
    GGTTACCCAG AGAAGTCTGA AGATGAAAAG CAGATGTTGG CAAAGAAGCT TGAGCAGAAA
    GCGAAGGGAG AAGGAATTCC CACAACAGCC AAGTTAAAAG TGGATGAATT TGAATCCAAT
    GTCAATGAGA TCAAAGATCC CTACCCATCC GCAGACTTCC CAGGTGATGA TGAGGAGGAT
    GAGCCTGAAA TCCCCCTGAG TCCTCGGCCA CGTCCTCTGG CAGAGCTGCA GCTGAAAGAG
    AAGGCTGTGC CTATGCCTGA AGCCAGCTCC TTCTTCATCT TCAGCCCAAC GAATAAGTTT
    CGGATGCTGT GCCACAGAAT CGTCAACGCC ACTTGGTTCA CCAACTTCAT CCTGCTCTTC
    ATCTTGCTCA GCAGCATTTC CCTGGCAGCT GAGGATCCCA TCCGAGCCGA GTCATTCCGC
    AACCAGATTC TTGGATACTT TGACATCGGT TTCACTTCTG TCTTCACTGT TGAAATCGTC
    TTGAAGATGA CAGCATATGG TGCTTTCCTG CACAAAGGCT CCTTCTGCAG GAACTCGTTC
    AACATCCTCG ACTTGCTGGT GGTTGCTGTG TCTCTCATCT CCATGGGATT TGAGTCCAGC
    ACAATCTCAG TGGTGAAGAT CCTCCGGGTG CTGAGGGTGC TGAGGCCTCT GCGAGCCATT
    AACAGGGCAA AGGGGCTGAA GCACGTGGTT CAGTGTGTGT TCGTGGCCAT CAAGACCATT
    GGTAACATTG TGGTCGTTAC GACGTTGCTG CAGTTCATGT TTGCCTGCAT TGGGGTGCAG
    CTCTTCAAGG GCAAGTTTTA CAGCTGCACA GATCCCTCCA AGCTAACAGA GAAGGAGTGT
    AGGGGGCATT TCATCAACTA CGTGGATGGG GACCCAACGC AGATCGAGCT GAAGGAGCGG
    GTCTGGTTCC ACAACGCCTT TCACTTCAAC AATGTCCTCT CAGCCATGAT GTCGCTTTTC
    ACTGTTTCCA CCTTCGAGGG CTGGCCAGAG CTGCTGTACA GGGCCATTGA CACTAACGAT
    GAGAATAAAG GCCCTATCTA CAACTACCGG GTGGAGATCG CGATGTTCTT CATCATCTAC
    ATCATCCTCA TTGCCTTCTT CATGATGAAC ATCTTTGTGG GCTTTGTCAT TGTCACCTTC
    CAGGAGCAGG GGGAGAGCGA GTACAAGAAC TGTGAGCTGG ACAAGAACCA GCGCCAGTGT
    GTGCAGTACG CACTGAAGGC TCGCCCGCTG CGGCGGTACA TCCCCAAGAA CCCCTACCAA
    TACCAGATCT GGTACGTGGT CACCTCCTCC TACTTCGAGT ACCTCATGTT CTTCCTGATC
    ATGCTGAACA CCATCTGCCT GGGCATGCAG CATTACAACC AGTCTGCGGA AATGAACCAC
    GTCTCAGACA TCCTCAATGT GGCCTTCACC GTCCTCTTCA CCCTGGAGAT GATCCTCAAG
    CTCATGGCCT TTAAAGCCAA GGGCTACTTT GGGGACCCCT GGAATGTCTT CGACTTCCTC
    ATAGTGATTG GCAGCATCAT TGATGTCATT CTCAGCGAGA TTGATACTGT TCTTGCATCC
    AGTGGTGGAT TGTACTGCCT CGGCGGAGGT TGTGATAGCA TTGACCCGGA TGACAACTCC
    CGTGTCTCCA TCACGTTCTT CCGGCTGTTC CGAGTGATGC GGCTGGTGAA GCTGCTGAGC
    CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG ACCTTCATCA AATCCTTCCA GGCTCTGCCC
    TATGTTGCCC TGCTGATCGT GATGCTGTTC TTCATCTATG CTGTGATCGG GATGCAGATG
    TTTGGGAAGA TTGCCATGGT GGACGGAACC CAGATCAACC GAAACAACAA CTTCCAAACC
    TTCCCACAAG CTGTGCTGCT GCTCTTCAGG TGTGCCACGG GGGAGGCCTG GCAGGAGATC
    CTGCTGGACT GCAGCTATGG GAAGCGGTGC GACCCTGAGT CTGACTACGC GGAGGGCGAG
    GAGTACACCT GTGGCACGGG CTTTGCCTAC TTCTATTTCA TCAGCTTCTA CATGCTCTGT
    GCCTTTCTGA TCATTAACCT TTTTGTGGCT GTCATCATGG ACAACTTTGA CTACCTCACA
    CGTGACTGGT CCATCCTGGG GCCCCACCAC CTGGATGAGT TCAAACGGAT CTGGGCTGAG
    TACGACCCTG AAGCCAAGGG CAGGATTAAG CACTTGGATG TGGTGACCCT GCTGAGACGT
    ATCCAACCTC CCCTGGGCTT TGGGAAGTTC TGCCCTCACC GTGTAGCTTG TAAGCGCCTG
    GTGTGCATGA ACATGCCCCT GAACAGCGAT GGCACTGTCA CCTTCAATGC AACCCTCTTT
    GCGCTGGTGA GGACAGCCCT CAAAATCAAG ACAGAAGGTA ACTTTGAACA GGCCAATGAG
    GAGCTCAGAA CCATTATCAA AAAAATCTGG AAGCGAACCA GTATGAAGCT TTTGGACCAG
    GTCATCCCAC CTATTGGAGA TGATGAGGTG ACAGTGGGCA AATTCTATGC TACTTTCCTC
    ATCCAAGAGC ATTTCCGAAA GTTCATGAAG CGCCAGGAGG AGTACTATGG GTACCGTCCC
    AAGAAGAACC CTGTAGAGAT CCAGGCTGGG CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT
    GAAATTCATC GAGCCATCTC TGGGGACCTG ACTGCTGAGG AGGAGCTGGA GAGGGCAATG
    GTGGAAGCTG CCATGGAGGA GGGGATATAC AGGAGGACGG GAGGTTTGTT TGGCCAGGTG
    GACAGCTTCC TGGAGCCGAG CAGCCCCCTG CAGCCCCACG TGGCCAGCCA GAGGCCCCTG
    CAGTTCACAG AGGTGGGCAG CGAAGACCTC GATTCCCCCG TCTTCCTCGA CGACTTCCCC
    CAGGAAGGCA ACACCAATGT CAACAACGCC AACTGGCAGC AGGGAGCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa46362
    Clone ID Related Accession (Same CDS sequence) XM_015298692.1
    Accession Version XM_015298692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4998bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_456028.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
    AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG
    AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC
    CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT
    GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT
    GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT
    CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG
    ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT
    GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA
    AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA
    CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG
    AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG
    TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT
    GCCATGCTGA CCGTCTTCCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG
    GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT
    GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG
    CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG
    GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT
    GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA
    ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC
    CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG
    CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG
    CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC
    GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG
    TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC
    AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT
    GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTGCT GGGCATGCAG
    CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC
    TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA
    ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC
    TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT
    GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG
    GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG
    ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG
    TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA
    GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT
    CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC
    TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT
    TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG
    GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC
    ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC
    AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT
    CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG
    AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG
    TGTGTGTTCG TGGCCATCAG AACCATTGGT AACATTGTGA TTGTTACCAC TTTGCTGCAG
    TTCATGTTTG CCTGCATTGG AGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT
    CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC
    CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT
    GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG
    CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG
    GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC
    TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT
    GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG
    CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC
    TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT
    TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC
    CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG
    GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC
    AGCGAGATTG ATACTGTTCT TGCATCCAGT GGTGGATTGT ACTGCCTCGG CGGAGGTTGT
    GATAGCATTG ACCCGGATGA CAACTCCCGT GTCTCCATCA CGTTCTTCCG GCTGTTCCGA
    GTGATGCGGC TGGTGAAGCT GCTGAGCCGG GGAGAAGGCG TCAGGACCCT CCTGTGGACC
    TTCATCAAAT CCTTCCAGGC TCTGCCCTAT GTTGCCCTGC TGATCGTGAT GCTGTTCTTC
    ATCTATGCTG TGATCGGGAT GCAGATGTTT GGGAAGATTG CCATGGTGGA CGGAACCCAG
    ATCAACCGAA ACAACAACTT CCAAACCTTC CCCCAAGCTG TGCTGCTGCT CTTCAGGTGT
    GCCACGGGGG AGGCCTGGCA GGAGATCCTG CTGGACTGCA GCTATGGGAA GCGGTGCGAC
    CCTGAGTCTG ACTACGCGGA GGGCGAGGAG TACACCTGTG GCACGGGCTT TGCCTACTTC
    TATTTCATCA GCTTCTACAT GCTCTGTGCC TTTCTGATCA TTAACCTTTT TGTGGCTGTC
    ATCATGGACA ACTTTGACTA CCTGACACGT GACTGGTCCA TCCTGGGGCC CCACCACCTG
    GATGAGTTCA AACGGATCTG GGCTGAGTAC GACCCTGAAG CCAAGGGCAG GATTAAGCAC
    TTGGATGTGG TGACCCTGCT GAGACGTATC CAACCTCCCC TGGGCTTTGG GAAGTTCTGC
    CCTCACCGTG TAGCTTGTAA GCGCCTGGTG TGCATGAACA TGCCCCTGAA CAGCGATGGC
    ACTGTCACCT TCAATGCAAC CCTCTTTGCG CTGGTGAGGA CAGCCCTCAA AATCAAGACA
    GAAGGTAACT TTGAACAGGC CAATGAGGAG CTCAGAACCA TTATCAAAAA AATCTGGAAG
    CGAACCAGTA TGAAGCTTTT GGACCAGGTC ATCCCACCTA TTGGAGATGA TGAGGTGACA
    GTGGGCAAAT TCTATGCTAC TTTCCTCATC CAAGAGCATT TCCGAAAGTT CATGAAGCGC
    CAGGAGGAGT ACTATGGGTA CCGTCCCAAG AAGAACCCTG TAGAGATCCA GGCTGGGCTG
    AGAAGCATTG AAGAAGAAGC TGCTCCTGAA ATTCATCGAG CCATCTCTGG GGACCTGACT
    GCTGAGGAGG AGCTGGAGAG GGCAATGGTG GAAGCTGCCA TGGAGGAGGG GATATACAGG
    AGGACGGGAG GTTTGTTTGG CCAGGTGGAC AGCTTCCTGG AGCCGAGCAG CCCCCTGCAG
    CCCCACGTGG CCAGCCAGAG GCCCCTGCAG TTCACAGAGG TGGGCAGCGA AGACCTCGAT
    TCCCCCGTCT TCCTCGACGA CTTCCCCCAG GAAGGCAACA CCAATGTCAA CAACGCCAAC
    TGGCAGCAGG GAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154178.1
    CDS153..5150
    Translation

    Target ORF information:

    RefSeq Version XM_015298692.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
    AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG
    AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC
    CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT
    GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT
    GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT
    CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG
    ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT
    GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA
    AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA
    CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG
    AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG
    TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT
    GCCATGCTGA CCGTCTTCCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG
    GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT
    GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG
    CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG
    GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT
    GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA
    ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC
    CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG
    CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG
    CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC
    GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG
    TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC
    AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT
    GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTGCT GGGCATGCAG
    CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC
    TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA
    ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC
    TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT
    GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG
    GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG
    ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG
    TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA
    GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT
    CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC
    TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT
    TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG
    GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC
    ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC
    AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT
    CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG
    AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG
    TGTGTGTTCG TGGCCATCAG AACCATTGGT AACATTGTGA TTGTTACCAC TTTGCTGCAG
    TTCATGTTTG CCTGCATTGG AGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT
    CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC
    CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT
    GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG
    CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG
    GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC
    TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT
    GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG
    CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC
    TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT
    TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC
    CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG
    GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC
    AGCGAGATTG ATACTGTTCT TGCATCCAGT GGTGGATTGT ACTGCCTCGG CGGAGGTTGT
    GATAGCATTG ACCCGGATGA CAACTCCCGT GTCTCCATCA CGTTCTTCCG GCTGTTCCGA
    GTGATGCGGC TGGTGAAGCT GCTGAGCCGG GGAGAAGGCG TCAGGACCCT CCTGTGGACC
    TTCATCAAAT CCTTCCAGGC TCTGCCCTAT GTTGCCCTGC TGATCGTGAT GCTGTTCTTC
    ATCTATGCTG TGATCGGGAT GCAGATGTTT GGGAAGATTG CCATGGTGGA CGGAACCCAG
    ATCAACCGAA ACAACAACTT CCAAACCTTC CCCCAAGCTG TGCTGCTGCT CTTCAGGTGT
    GCCACGGGGG AGGCCTGGCA GGAGATCCTG CTGGACTGCA GCTATGGGAA GCGGTGCGAC
    CCTGAGTCTG ACTACGCGGA GGGCGAGGAG TACACCTGTG GCACGGGCTT TGCCTACTTC
    TATTTCATCA GCTTCTACAT GCTCTGTGCC TTTCTGATCA TTAACCTTTT TGTGGCTGTC
    ATCATGGACA ACTTTGACTA CCTGACACGT GACTGGTCCA TCCTGGGGCC CCACCACCTG
    GATGAGTTCA AACGGATCTG GGCTGAGTAC GACCCTGAAG CCAAGGGCAG GATTAAGCAC
    TTGGATGTGG TGACCCTGCT GAGACGTATC CAACCTCCCC TGGGCTTTGG GAAGTTCTGC
    CCTCACCGTG TAGCTTGTAA GCGCCTGGTG TGCATGAACA TGCCCCTGAA CAGCGATGGC
    ACTGTCACCT TCAATGCAAC CCTCTTTGCG CTGGTGAGGA CAGCCCTCAA AATCAAGACA
    GAAGGTAACT TTGAACAGGC CAATGAGGAG CTCAGAACCA TTATCAAAAA AATCTGGAAG
    CGAACCAGTA TGAAGCTTTT GGACCAGGTC ATCCCACCTA TTGGAGATGA TGAGGTGACA
    GTGGGCAAAT TCTATGCTAC TTTCCTCATC CAAGAGCATT TCCGAAAGTT CATGAAGCGC
    CAGGAGGAGT ACTATGGGTA CCGTCCCAAG AAGAACCCTG TAGAGATCCA GGCTGGGCTG
    AGAAGCATTG AAGAAGAAGC TGCTCCTGAA ATTCATCGAG CCATCTCTGG GGACCTGACT
    GCTGAGGAGG AGCTGGAGAG GGCAATGGTG GAAGCTGCCA TGGAGGAGGG GATATACAGG
    AGGACGGGAG GTTTGTTTGG CCAGGTGGAC AGCTTCCTGG AGCCGAGCAG CCCCCTGCAG
    CCCCACGTGG CCAGCCAGAG GCCCCTGCAG TTCACAGAGG TGGGCAGCGA AGACCTCGAT
    TCCCCCGTCT TCCTCGACGA CTTCCCCCAG GAAGGCAACA CCAATGTCAA CAACGCCAAC
    TGGCAGCAGG GAGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa19560
    Clone ID Related Accession (Same CDS sequence) NM_001305147.1
    Accession Version NM_001305147.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-11
    Organism Gallus gallus(chicken)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000168.1. On Feb 25, 2015 this sequence version replaced XM_419259.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on protein alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201367, SAMEA2201368 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
    AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG
    AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC
    CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT
    GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT
    GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT
    CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG
    ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT
    GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA
    AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA
    CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG
    AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG
    TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT
    GCCATGCTGA CTGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG
    GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT
    GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG
    CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG
    GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT
    GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA
    ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC
    CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG
    CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG
    CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC
    GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG
    TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC
    AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT
    GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTACT GGGCATGCAG
    CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC
    TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA
    ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC
    TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT
    GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG
    GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG
    ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG
    TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA
    GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT
    CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC
    TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT
    TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG
    GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC
    ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC
    AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT
    CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG
    AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG
    TGTGTGTTCG TGGCCATCAA GACCATTGGT AACATTGTGG TCGTTACGAC GTTGCTGCAG
    TTCATGTTTG CCTGCATTGG GGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT
    CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC
    CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT
    GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG
    CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG
    GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC
    TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT
    GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG
    CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC
    TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT
    TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC
    CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG
    GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC
    AGCGAGATTG ATACTGTTCT TGCATCCAGT GGTGGATTGT ACTGCCTCGG CGGAGGTTGT
    GATAGCATTG ACCCGGATGA CAACTCCCGT GTCTCCATCA CGTTCTTCCG GCTGTTCCGA
    GTGATGCGGC TGGTGAAGCT GCTGAGCCGG GGAGAAGGCG TCAGGACCCT CCTGTGGACC
    TTCATCAAAT CCTTCCAGGC TCTGCCCTAT GTTGCCCTGC TGATCGTGAT GCTGTTCTTC
    ATCTATGCTG TGATCGGGAT GCAGATGTTT GGGAAGATTG CCATGGTGGA CGGAACCCAG
    ATCAACCGAA ACAACAACTT CCAAACCTTC CCACAAGCTG TGCTGCTGCT CTTCAGGTGT
    GCCACGGGGG AGGCCTGGCA GGAGATCCTG CTGGACTGCA GCTATGGGAA GCGGTGCGAC
    CCTGAGTCTG ACTACGCGGA GGGCGAGGAG TACACCTGTG GCACGGGCTT TGCCTACTTC
    TATTTCATCA GCTTCTACAT GCTCTGTGCC TTTCTGATCA TTAACCTTTT TGTGGCTGTC
    ATCATGGACA ACTTTGACTA CCTCACACGT GACTGGTCCA TCCTGGGGCC CCACCACCTG
    GATGAGTTCA AACGGATCTG GGCTGAGTAC GACCCTGAAG CCAAGGGCAG GATTAAGCAC
    TTGGATGTGG TGACCCTGCT GAGACGTATC CAACCTCCCC TGGGCTTTGG GAAGTTCTGC
    CCTCACCGTG TAGCTTGTAA GCGCCTGGTG TGCATGAACA TGCCCCTGAA CAGCGATGGC
    ACTGTCACCT TCAATGCAAC CCTCTTTGCG CTGGTGAGGA CAGCCCTCAA AATCAAGACA
    GAAGGTAACT TTGAACAGGC CAATGAGGAG CTCAGAACCA TTATCAAAAA AATCTGGAAG
    CGAACCAGTA TGAAGCTTTT GGACCAGGTC ATCCCACCTA TTGGAGATGA TGAGGTGACA
    GTGGGCAAAT TCTATGCTAC TTTCCTCATC CAAGAGCATT TCCGAAAGTT CATGAAGCGC
    CAGGAGGAGT ACTATGGGTA CCGTCCCAAG AAGAACCCTG TAGAGATCCA GGCTGGGCTG
    AGAAGCATTG AAGAAGAAGC TGCTCCTGAA ATTCATCGAG CCATCTCTGG GGACCTGACT
    GCTGAGGAGG AGCTGGAGAG GGCAATGGTG GAAGCTGCCA TGGAGGAGGG GATATACAGG
    AGGACGGGAG GTTTGTTTGG CCAGGTGGAC AGCTTCCTGG AGCCGAGCAG CCCCCTGCAG
    CCCCACGTGG CCAGCCAGAG GCCCCTGCAG TTCACAGAGG TGGGCAGCGA AGACCTCGAT
    TCCCCCGTCT TCCTCGACGA CTTCCCCCAG GAAGGCAACA CCAATGTCAA CAACGCCAAC
    TGGCAGCAGG GGTTGGAATA CGAGGATGAG GTGCTGAGGA GAGCAGTGCC CAGGCCTCCA
    GTGCGGGAGC CTGACCTCCC CATCCCTTTG GAGAGGCTGC GGCGGAGGCG AGGAGCTGCC
    TCTGAGCATC ACCCAGCATC GGGGCCATCC CTCCATCCCC AGCAGGGCAG CACAGTGACA
    GCAGCAGCAT CCAGCTCAGA ACACCGCTGG CATGAAAGCA TTGACACAAA CACATCTTCT
    ACCCCCGCCA TCACCTTCCT CATTCAAGAG GCTCTGATCT CCGGAGGCCT CGCTGCTCTG
    GCCCGCGACC CATCCTTTGT TGCAGTGACC AGGGATGAGA TGGCAGCCAG CAGCCAGATG
    GAGATGGACG AAGTGGAGAA AGCTGCAGTG GAGCTGCTAC AGGGAAGAGA AGCACTGCAG
    GACACAGAAA TCAGCTCTGC TCCCCGGGAC ACCTTGGCTG CATCAGCAGT CAGCACCCCG
    GGGCTCAGCG TGGCCGCATC CCATCAGACC AGCTCTGCTG CTCGCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001292076.1
    CDS130..5640
    Translation

    Target ORF information:

    RefSeq Version NM_001305147.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001305147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
    AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG
    AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC
    CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT
    GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT
    GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT
    CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG
    ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT
    GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA
    AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA
    CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG
    AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG
    TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT
    GCCATGCTGA CTGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG
    GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT
    GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG
    CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG
    GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT
    GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA
    ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC
    CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG
    CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG
    CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC
    GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG
    TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC
    AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT
    GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTACT GGGCATGCAG
    CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC
    TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA
    ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC
    TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT
    GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG
    GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG
    ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG
    TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA
    GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT
    CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC
    TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT
    TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG
    GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC
    ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC
    AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT
    CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG
    AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG
    TGTGTGTTCG TGGCCATCAA GACCATTGGT AACATTGTGG TCGTTACGAC GTTGCTGCAG
    TTCATGTTTG CCTGCATTGG GGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT
    CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC
    CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT
    GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG
    CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG
    GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC
    TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT
    GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG
    CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC
    TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT
    TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC
    CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG
    GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC
    AGCGAGATTG ATACTGTTCT TGCATCCAGT GGTGGATTGT ACTGCCTCGG CGGAGGTTGT
    GATAGCATTG ACCCGGATGA CAACTCCCGT GTCTCCATCA CGTTCTTCCG GCTGTTCCGA
    GTGATGCGGC TGGTGAAGCT GCTGAGCCGG GGAGAAGGCG TCAGGACCCT CCTGTGGACC
    TTCATCAAAT CCTTCCAGGC TCTGCCCTAT GTTGCCCTGC TGATCGTGAT GCTGTTCTTC
    ATCTATGCTG TGATCGGGAT GCAGATGTTT GGGAAGATTG CCATGGTGGA CGGAACCCAG
    ATCAACCGAA ACAACAACTT CCAAACCTTC CCACAAGCTG TGCTGCTGCT CTTCAGGTGT
    GCCACGGGGG AGGCCTGGCA GGAGATCCTG CTGGACTGCA GCTATGGGAA GCGGTGCGAC
    CCTGAGTCTG ACTACGCGGA GGGCGAGGAG TACACCTGTG GCACGGGCTT TGCCTACTTC
    TATTTCATCA GCTTCTACAT GCTCTGTGCC TTTCTGATCA TTAACCTTTT TGTGGCTGTC
    ATCATGGACA ACTTTGACTA CCTCACACGT GACTGGTCCA TCCTGGGGCC CCACCACCTG
    GATGAGTTCA AACGGATCTG GGCTGAGTAC GACCCTGAAG CCAAGGGCAG GATTAAGCAC
    TTGGATGTGG TGACCCTGCT GAGACGTATC CAACCTCCCC TGGGCTTTGG GAAGTTCTGC
    CCTCACCGTG TAGCTTGTAA GCGCCTGGTG TGCATGAACA TGCCCCTGAA CAGCGATGGC
    ACTGTCACCT TCAATGCAAC CCTCTTTGCG CTGGTGAGGA CAGCCCTCAA AATCAAGACA
    GAAGGTAACT TTGAACAGGC CAATGAGGAG CTCAGAACCA TTATCAAAAA AATCTGGAAG
    CGAACCAGTA TGAAGCTTTT GGACCAGGTC ATCCCACCTA TTGGAGATGA TGAGGTGACA
    GTGGGCAAAT TCTATGCTAC TTTCCTCATC CAAGAGCATT TCCGAAAGTT CATGAAGCGC
    CAGGAGGAGT ACTATGGGTA CCGTCCCAAG AAGAACCCTG TAGAGATCCA GGCTGGGCTG
    AGAAGCATTG AAGAAGAAGC TGCTCCTGAA ATTCATCGAG CCATCTCTGG GGACCTGACT
    GCTGAGGAGG AGCTGGAGAG GGCAATGGTG GAAGCTGCCA TGGAGGAGGG GATATACAGG
    AGGACGGGAG GTTTGTTTGG CCAGGTGGAC AGCTTCCTGG AGCCGAGCAG CCCCCTGCAG
    CCCCACGTGG CCAGCCAGAG GCCCCTGCAG TTCACAGAGG TGGGCAGCGA AGACCTCGAT
    TCCCCCGTCT TCCTCGACGA CTTCCCCCAG GAAGGCAACA CCAATGTCAA CAACGCCAAC
    TGGCAGCAGG GGTTGGAATA CGAGGATGAG GTGCTGAGGA GAGCAGTGCC CAGGCCTCCA
    GTGCGGGAGC CTGACCTCCC CATCCCTTTG GAGAGGCTGC GGCGGAGGCG AGGAGCTGCC
    TCTGAGCATC ACCCAGCATC GGGGCCATCC CTCCATCCCC AGCAGGGCAG CACAGTGACA
    GCAGCAGCAT CCAGCTCAGA ACACCGCTGG CATGAAAGCA TTGACACAAA CACATCTTCT
    ACCCCCGCCA TCACCTTCCT CATTCAAGAG GCTCTGATCT CCGGAGGCCT CGCTGCTCTG
    GCCCGCGACC CATCCTTTGT TGCAGTGACC AGGGATGAGA TGGCAGCCAG CAGCCAGATG
    GAGATGGACG AAGTGGAGAA AGCTGCAGTG GAGCTGCTAC AGGGAAGAGA AGCACTGCAG
    GACACAGAAA TCAGCTCTGC TCCCCGGGAC ACCTTGGCTG CATCAGCAGT CAGCACCCCG
    GGGCTCAGCG TGGCCGCATC CCATCAGACC AGCTCTGCTG CTCGCCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa19561
    Clone ID Related Accession (Same CDS sequence) NM_001305148.1
    Accession Version NM_001305148.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-11
    Organism Gallus gallus(chicken)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000168.1. On Feb 25, 2015 this sequence version replaced XM_004934808.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on protein alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201367, SAMEA2201369 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
    AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG
    AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC
    CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT
    GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT
    GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT
    CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG
    ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT
    GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA
    AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA
    CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG
    AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG
    TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT
    GCCATGCTGA CTGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG
    GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT
    GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG
    CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG
    GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT
    GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA
    ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC
    CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG
    CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG
    CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC
    GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG
    TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC
    AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT
    GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTACT GGGCATGCAG
    CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC
    TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA
    ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC
    TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT
    GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG
    GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG
    ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG
    TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA
    GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT
    CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC
    TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT
    TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG
    GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC
    ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC
    AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT
    CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG
    AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG
    TGTGTGTTCG TGGCCATCAA GACCATTGGT AACATTGTGG TCGTTACGAC GTTGCTGCAG
    TTCATGTTTG CCTGCATTGG GGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT
    CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC
    CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT
    GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG
    CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG
    GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC
    TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT
    GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG
    CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC
    TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT
    TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC
    CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG
    GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC
    AGCGAGATTG ATGACCCGGA TGACAACTCC CGTGTCTCCA TCACGTTCTT CCGGCTGTTC
    CGAGTGATGC GGCTGGTGAA GCTGCTGAGC CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG
    ACCTTCATCA AATCCTTCCA GGCTCTGCCC TATGTTGCCC TGCTGATCGT GATGCTGTTC
    TTCATCTATG CTGTGATCGG GATGCAGATG TTTGGGAAGA TTGCCATGGT GGACGGAACC
    CAGATCAACC GAAACAACAA CTTCCAAACC TTCCCACAAG CTGTGCTGCT GCTCTTCAGG
    TGTGCCACGG GGGAGGCCTG GCAGGAGATC CTGCTGGACT GCAGCTATGG GAAGCGGTGC
    GACCCTGAGT CTGACTACGC GGAGGGCGAG GAGTACACCT GTGGCACGGG CTTTGCCTAC
    TTCTATTTCA TCAGCTTCTA CATGCTCTGT GCCTTTCTGA TCATTAACCT TTTTGTGGCT
    GTCATCATGG ACAACTTTGA CTACCTCACA CGTGACTGGT CCATCCTGGG GCCCCACCAC
    CTGGATGAGT TCAAACGGAT CTGGGCTGAG TACGACCCTG AAGCCAAGGG CAGGATTAAG
    CACTTGGATG TGGTGACCCT GCTGAGACGT ATCCAACCTC CCCTGGGCTT TGGGAAGTTC
    TGCCCTCACC GTGTAGCTTG TAAGCGCCTG GTGTGCATGA ACATGCCCCT GAACAGCGAT
    GGCACTGTCA CCTTCAATGC AACCCTCTTT GCGCTGGTGA GGACAGCCCT CAAAATCAAG
    ACAGAAGGTA ACTTTGAACA GGCCAATGAG GAGCTCAGAA CCATTATCAA AAAAATCTGG
    AAGCGAACCA GTATGAAGCT TTTGGACCAG GTCATCCCAC CTATTGGAGA TGATGAGGTG
    ACAGTGGGCA AATTCTATGC TACTTTCCTC ATCCAAGAGC ATTTCCGAAA GTTCATGAAG
    CGCCAGGAGG AGTACTATGG GTACCGTCCC AAGAAGAACC CTGTAGAGAT CCAGGCTGGG
    CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT GAAATTCATC GAGCCATCTC TGGGGACCTG
    ACTGCTGAGG AGGAGCTGGA GAGGGCAATG GTGGAAGCTG CCATGGAGGA GGGGATATAC
    AGGAGGACGG GAGGTTTGTT TGGCCAGGTG GACAGCTTCC TGGAGCCGAG CAGCCCCCTG
    CAGCCCCACG TGGCCAGCCA GAGGCCCCTG CAGTTCACAG AGGTGGGCAG CGAAGACCTC
    GATTCCCCCG TCTTCCTCGA CGACTTCCCC CAGGAAGGCA ACACCAATGT CAACAACGCC
    AACTGGCAGC AGGGGTTGGA ATACGAGGAT GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT
    CCAGTGCGGG AGCCTGACCT CCCCATCCCT TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT
    GCCTCTGAGC ATCACCCAGC ATCGGGGCCA TCCCTCCATC CCCAGCAGGG CAGCACAGTG
    ACAGCAGCAG CATCCAGCTC AGAACACCGC TGGCATGAAA GCATTGACAC AAACACATCT
    TCTACCCCCG CCATCACCTT CCTCATTCAA GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT
    CTGGCCCGCG ACCCATCCTT TGTTGCAGTG ACCAGGGATG AGATGGCAGC CAGCAGCCAG
    ATGGAGATGG ACGAAGTGGA GAAAGCTGCA GTGGAGCTGC TACAGGGAAG AGAAGCACTG
    CAGGACACAG AAATCAGCTC TGCTCCCCGG GACACCTTGG CTGCATCAGC AGTCAGCACC
    CCGGGGCTCA GCGTGGCCGC ATCCCATCAG ACCAGCTCTG CTGCTCGCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001292077.1
    CDS130..5583
    Translation

    Target ORF information:

    RefSeq Version NM_001305148.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001305148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGGAGCCTG CGTCCCACCA GGATGATGTG AAGAAGAGGC AACAGAAGGA GAAATCCAAG 
    AAGCCGGTGC CACCAGTAGC CCCTCGGCCA CCCAGGGCTC TGTTCTGCTT AACCCTACAG
    AACCCACTGC GGAAGGCATG CATCAGCATC GTGGAGTGGA AACCTTTCGA GATCATCATC
    CTTCTGACCA TCTTTGCCAA CTGTGTTGCC CTGGCCATCT ACCAGCCCAT GCCGGAGGAT
    GACACCAACG TGGCCAACTC CAGCCTGGAG AAGTTGGAAT ATGTCTTCCT GATCTTCTTT
    GCCATCGAGG CCATGCTCAA GATAATAGCT TACGGGTTTC TGTTCCATAC GGACGCCTAT
    CTCCGAAACG GCTGGAACGT CCTCGACTTC TCCATCGTTT CCCTGGGGCT TGTCACCATG
    ACCCTGGAGC AGATCAATGC AAAGGAGGGA GGGTCTTTGG GAGGGAAAGG TGGCTTTGAT
    GTGAAGGCCC TGCGAGCGTT TCGTGTGCTG AGACCTCTGC GCCTGGTGTC CGGAGTGCCA
    AGTCTTCAGG TTGTCCTGAA CTCCATTATC AAAGCCATGG TGCCTCTGCT CCACATTGCA
    CTGCTCGTCC TCTTCATGAT CATCATCTAT GCCATCGTGG GGCAAGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTACCTTGGG ACAGATGTGA TCGCCACGGT GGGGTCAGAG
    AAGCCAGCCC CCTGCACAAC CTCTGGCCAT GGGCGTCACT GCTCCATCAA TGGCACTGAG
    TGCCGTGGTG GCTGGCCGGG ACCCAACAAT GGCATCACTC ACTTCGATAA CTTTGGCTTT
    GCCATGCTGA CTGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGAAGT GCTCTACTGG
    GTTAACGATG CCATTGGAAA TGAATGGCCA TGGATCTACT TTGTCAGCCT TATTTTGCTT
    GGTTCCTTCT TTGTTCTCAA CCTGGTGCTG GGGGTGCTCA GTGGGGAGTT CACCAAAGAG
    CGTGAGAAGG CCAAGTCACG AGGCACGTTT CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGATATGA AGGGCTACAT GGACTGGATC ACCCATGCTG AGGTGATGGA CAGTGACCGG
    GCCAGAGGAG AAGGTATGAT GCCGTTGGAT GAAGGAGGGT CAGAGACAGA GAGCTTGTAT
    GAAATTGAGG GCATGAACAA ATGGATTCTC TACTTCCGGC AGTGGAGGCG TTGGAACCGA
    ATGTTTCGTA GGAAGTGCAG GGATGTGGTG AAATCCAAGT TCTTCTACTG GCTGGTCATC
    CTGCTGGTGG CACTGAATAC CCTCTCCATC GCCTCTGAGC ACCATTTCCA GCCCGAATGG
    CTGACGATTG TACAAGATAA TGCCAACCGG GTGCTGCTTG CTCTCTTTGT GGCCGAAATG
    CTGCTGAAGA TGTATGCACT GGGCCTGCGC CAGTACTTCA TGTCCCTCTT CAACCGCTTC
    GACTGCTTTG TTGTGTGTGC GGGGGTCCTG GAGATCATCC TGGTGGAGCT CAGCACGTTG
    TCACCCCTGG GCATCTCTGT GCTGCGCTGC ATCCGGCTGC TCCGCATCTT CAAGATCACC
    AGGTATTGGA CATCACTGAG TAACCTGGTG GCCTCTCTGC TCAACTCGGT GCGCTCCATT
    GCCTCTCTGC TCCTTCTCCT CTTCCTCTTC ATCATTGTTT TTGCCCTACT GGGCATGCAG
    CTCTTTGGGG GCATGTATGA CTTCGAGGAC ATGGAGGTGC GACGCAGCAC ATTCGACAAC
    TTCCCCCAGG CCCTCATCAG TGTCTTCCAG ATCCTGACTG GAGAGGACTG GAATTCAATA
    ATGTACAATG GGATAATGGC TTATGGGGGC CCATCCTTCC CTGGCATGCT GGTCTGCATC
    TACTTCATCA TCCTCTTTGT CTGTGGGAAC TATATCCTCC TCAACGTCTT CCTGGCCATT
    GCAGTTGATA ATCTGGCTGA GGCTGAGAGC CTGACCTCAG CTCAGAAGGC CAAAGCTGAG
    GAACGCAAGC GGCGGAAGAT GTCCAGAGGT TACCCAGAGA AGTCTGAAGA TGAAAAGCAG
    ATGTTGGCAA AGAAGCTTGA GCAGAAAGCG AAGGGAGAAG GAATTCCCAC AACAGCCAAG
    TTAAAAGTGG ATGAATTTGA ATCCAATGTC AATGAGATCA AAGATCCCTA CCCATCCGCA
    GACTTCCCAG GTGATGATGA GGAGGATGAG CCTGAAATCC CCCTGAGTCC TCGGCCACGT
    CCTCTGGCAG AGCTGCAGCT GAAAGAGAAG GCTGTGCCTA TGCCTGAAGC CAGCTCCTTC
    TTCATCTTCA GCCCAACGAA TAAGTTTCGG ATGCTGTGCC ACAGAATCGT CAACGCCACT
    TGGTTCACCA ACTTCATCCT GCTCTTCATC TTGCTCAGCA GCATTTCCCT GGCAGCTGAG
    GATCCCATCC GAGCCGAGTC ATTCCGCAAC CAGATTCTTG GATACTTTGA CATCGGTTTC
    ACTTCTGTCT TCACTGTTGA AATCGTCTTG AAGATGACAG CATATGGTGC TTTCCTGCAC
    AAAGGCTCCT TCTGCAGGAA CTCGTTCAAC ATCCTCGACT TGCTGGTGGT TGCTGTGTCT
    CTCATCTCCA TGGGATTTGA GTCCAGCACA ATCTCAGTGG TGAAGATCCT CCGGGTGCTG
    AGGGTGCTGA GGCCTCTGCG AGCCATTAAC AGGGCAAAGG GGCTGAAGCA CGTGGTTCAG
    TGTGTGTTCG TGGCCATCAA GACCATTGGT AACATTGTGG TCGTTACGAC GTTGCTGCAG
    TTCATGTTTG CCTGCATTGG GGTGCAGCTC TTCAAGGGCA AGTTTTACAG CTGCACAGAT
    CCCTCCAAGC TAACAGAGAA GGAGTGTAGG GGGCATTTCA TCAACTACGT GGATGGGGAC
    CCAACGCAGA TCGAGCTGAA GGAGCGGGTC TGGTTCCACA ACGCCTTTCA CTTCAACAAT
    GTCCTCTCAG CCATGATGTC GCTTTTCACT GTTTCCACCT TCGAGGGCTG GCCAGAGCTG
    CTGTACAGGG CCATTGACAC TAACGATGAG AATAAAGGCC CTATCTACAA CTACCGGGTG
    GAGATCGCGA TGTTCTTCAT CATCTACATC ATCCTCATTG CCTTCTTCAT GATGAACATC
    TTTGTGGGCT TTGTCATTGT CACCTTCCAG GAGCAGGGGG AGAGCGAGTA CAAGAACTGT
    GAGCTGGACA AGAACCAGCG CCAGTGTGTG CAGTACGCAC TGAAGGCTCG CCCGCTGCGG
    CGGTACATCC CCAAGAACCC CTACCAATAC CAGATCTGGT ACGTGGTCAC CTCCTCCTAC
    TTCGAGTACC TCATGTTCTT CCTGATCATG CTGAACACCA TCTGCCTGGG CATGCAGCAT
    TACAACCAGT CTGCGGAAAT GAACCACGTC TCAGACATCC TCAATGTGGC CTTCACCGTC
    CTCTTCACCC TGGAGATGAT CCTCAAGCTC ATGGCCTTTA AAGCCAAGGG CTACTTTGGG
    GACCCCTGGA ATGTCTTCGA CTTCCTCATA GTGATTGGCA GCATCATTGA TGTCATTCTC
    AGCGAGATTG ATGACCCGGA TGACAACTCC CGTGTCTCCA TCACGTTCTT CCGGCTGTTC
    CGAGTGATGC GGCTGGTGAA GCTGCTGAGC CGGGGAGAAG GCGTCAGGAC CCTCCTGTGG
    ACCTTCATCA AATCCTTCCA GGCTCTGCCC TATGTTGCCC TGCTGATCGT GATGCTGTTC
    TTCATCTATG CTGTGATCGG GATGCAGATG TTTGGGAAGA TTGCCATGGT GGACGGAACC
    CAGATCAACC GAAACAACAA CTTCCAAACC TTCCCACAAG CTGTGCTGCT GCTCTTCAGG
    TGTGCCACGG GGGAGGCCTG GCAGGAGATC CTGCTGGACT GCAGCTATGG GAAGCGGTGC
    GACCCTGAGT CTGACTACGC GGAGGGCGAG GAGTACACCT GTGGCACGGG CTTTGCCTAC
    TTCTATTTCA TCAGCTTCTA CATGCTCTGT GCCTTTCTGA TCATTAACCT TTTTGTGGCT
    GTCATCATGG ACAACTTTGA CTACCTCACA CGTGACTGGT CCATCCTGGG GCCCCACCAC
    CTGGATGAGT TCAAACGGAT CTGGGCTGAG TACGACCCTG AAGCCAAGGG CAGGATTAAG
    CACTTGGATG TGGTGACCCT GCTGAGACGT ATCCAACCTC CCCTGGGCTT TGGGAAGTTC
    TGCCCTCACC GTGTAGCTTG TAAGCGCCTG GTGTGCATGA ACATGCCCCT GAACAGCGAT
    GGCACTGTCA CCTTCAATGC AACCCTCTTT GCGCTGGTGA GGACAGCCCT CAAAATCAAG
    ACAGAAGGTA ACTTTGAACA GGCCAATGAG GAGCTCAGAA CCATTATCAA AAAAATCTGG
    AAGCGAACCA GTATGAAGCT TTTGGACCAG GTCATCCCAC CTATTGGAGA TGATGAGGTG
    ACAGTGGGCA AATTCTATGC TACTTTCCTC ATCCAAGAGC ATTTCCGAAA GTTCATGAAG
    CGCCAGGAGG AGTACTATGG GTACCGTCCC AAGAAGAACC CTGTAGAGAT CCAGGCTGGG
    CTGAGAAGCA TTGAAGAAGA AGCTGCTCCT GAAATTCATC GAGCCATCTC TGGGGACCTG
    ACTGCTGAGG AGGAGCTGGA GAGGGCAATG GTGGAAGCTG CCATGGAGGA GGGGATATAC
    AGGAGGACGG GAGGTTTGTT TGGCCAGGTG GACAGCTTCC TGGAGCCGAG CAGCCCCCTG
    CAGCCCCACG TGGCCAGCCA GAGGCCCCTG CAGTTCACAG AGGTGGGCAG CGAAGACCTC
    GATTCCCCCG TCTTCCTCGA CGACTTCCCC CAGGAAGGCA ACACCAATGT CAACAACGCC
    AACTGGCAGC AGGGGTTGGA ATACGAGGAT GAGGTGCTGA GGAGAGCAGT GCCCAGGCCT
    CCAGTGCGGG AGCCTGACCT CCCCATCCCT TTGGAGAGGC TGCGGCGGAG GCGAGGAGCT
    GCCTCTGAGC ATCACCCAGC ATCGGGGCCA TCCCTCCATC CCCAGCAGGG CAGCACAGTG
    ACAGCAGCAG CATCCAGCTC AGAACACCGC TGGCATGAAA GCATTGACAC AAACACATCT
    TCTACCCCCG CCATCACCTT CCTCATTCAA GAGGCTCTGA TCTCCGGAGG CCTCGCTGCT
    CTGGCCCGCG ACCCATCCTT TGTTGCAGTG ACCAGGGATG AGATGGCAGC CAGCAGCCAG
    ATGGAGATGG ACGAAGTGGA GAAAGCTGCA GTGGAGCTGC TACAGGGAAG AGAAGCACTG
    CAGGACACAG AAATCAGCTC TGCTCCCCGG GACACCTTGG CTGCATCAGC AGTCAGCACC
    CCGGGGCTCA GCGTGGCCGC ATCCCATCAG ACCAGCTCTG CTGCTCGCCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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