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CACNA1S cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol CACNA1S
Entrez Gene ID 100337204
Full Name calcium voltage-gated channel subunit alpha1 S
Synonyms caV1.1
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015475322.1 XP_015330808.1 voltage-dependent L-type calcium channel subunit alpha-1S
XM_024976574.1 XP_024832342.1 voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
XM_024976575.1 XP_024832343.1 voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
XM_015466951.1 XP_015322437.1 voltage-dependent L-type calcium channel subunit alpha-1S

Pathways from BioSystems


Homologies


CACNA1S XP_002802046.1
Rattus norvegicus (Norway rat) Cacna1s NP_446325.1
Homo sapiens (human) CACNA1S NP_000060.2
Pan troglodytes (chimpanzee) CACNA1S XP_525018.3
Canis lupus familiaris (dog) CACNA1S XP_848685.3
Bos taurus (cattle) CACNA1S XP_002694288.2
Mus musculus (house mouse) Cacna1s NP_001074492.1
Danio rerio (zebrafish) cacna1sa NP_001139622.1
Gallus gallus (chicken) CACNA1S XP_419259.3
Danio rerio (zebrafish) cacna1sb NP_999891.1

Gene Ontology

The following CACNA1S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223792 XM_024976574.1
Latest version!		 Bos taurus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223793 XM_024976575.1
Latest version!		 Bos taurus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa93088 XM_015466951.1
Latest version!		 Bos taurus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa93088 XM_015475322.1
Latest version!		 Bos taurus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa223792
Clone ID Related Accession (Same CDS sequence) XM_024976574.1
Accession Version XM_024976574.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5619bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG 
CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC 
ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC 
AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG 
GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG 
CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC 
GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC 
CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG 
AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG 
TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC 
TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA 
ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC 
TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG 
ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG 
ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG 
CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GGATCTTCAA GATCACCAAG 
TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC 
TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC 
CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG 
TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC 
TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG 
CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG 
GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG 
CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGCGTC CTGTGCCACC GCATTGTCAA TGCCACCTGG 
TTCACTAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCTTGGC CGCCGAGGAC 
CCCCTCCGGG CTGAGTCCGT GAGGAACCAG ATCCTCGGGT ATTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGGGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGG TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTCGAGTC CAGCACCATC TCCGTGGTGA AGATCCTGAG GGTCCTGAGG 
GTGCTCCGAC CGCTCCGGGC CATCAACAGA GCCAAAGGCC TGAAGCACGT GGTACAGTGT 
GTGTTCGTGG CCATCCGCAC CATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCTT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTG 
TCCAAGATGA CGGAGGAGGA GTGCAGGGGC TACTACTATG TGTACAAAGA TGGAGACCCC 
ACACAGATAG AGGTGCGTCC CCGCCAGTGG GTGCACAACG CCTTCCACTT CGACAACGTG 
CTTTCGGCCA TGATGTCCCT CTTCACCGTG TCCACCTTCG AGGGATGGCC TGAGCTGCTG 
TACAAGGCCA TTGACTCTCA TGAGGAGGAC AAGGGCCCCG TGTACAACCA CCGCGTGGAG 
ATGGCCATTT TCTTCATCAT CTACATCATC CTTATCGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTTG TCATCGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTGCAG TACGCCCTGA AGGCCCGGCC CCTGAGGTGC 
TACATCCCCA AGAACCCCTA CCAGTACCGG GTGTGGTACA TCGTCACCTC CTCCTACTTC 
GAGTACCTGA TGTTCGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCGG AGCAAATGAA CCACATCTCT GACATCCTCA ACGTAGCCTT CACCATCATC 
TTCACACTGG AGATGGTCCT CAAGCTCATG GCGTTCAAGG CCAGGGGCTA TTTTGGGGAC 
CCCTGGAACG TGTTTGACTT TCTGATTGTC ATCGGCAGCA TCATCGACGT CATCCTCAGT 
GAGATCGACA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG CGGCTGCGGG 
AACGTTGACC CGGACGAGAG CGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGTGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGCGCG GAGGGCGTGC GCACGCTGCT CTGGACTTTC 
ATCAAGTCCT TCCAGGCCCT GCCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCT TGGTGGACGG GACCCAGATC 
AACCGGAACA ACAACTTCCA GACCTTCCCC CAGGCGGTGC TGCTGCTCTT CAGGTGCGCC 
ACGGGGGAGG CGTGGCAGGA GATCCTGCTG GCCTGCAGCT ACGGCCAGCT GTGTGACCCC 
GAGTCGGACT ACGCCCCCGG GGAGGAGTAC ACCTGTGGCA CGGACTTCGC CTACTACTAC 
TTCCTCAGCT TCTACATGCT GTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCCGTCATC 
ATGGACAACT TTGACTACCT GACACGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATCTGGGC GGAGTACGAC CCGGAGGCTA CGGGCAGGAT CAAGCACCTG 
GACGTGGTGA CCCTGCTGAG GAGGATCCAG CCCCCGCTGG GCTTCGGGAA GTTCTGCCCC 
CACCGGGTAG CGTGTAAGCG GCTGGTGGGC ATGAACATGC CCCTCAACAG TGACGGCACG 
GTCACCTTCA ACGCCACGCT CTTCGCCCTG GTGCGCACAG CTCTGAAGAT CAAGACGGAA 
GGTAACTTCG AGCAGGCCAA CGAGGAGCTG CGAGCCATCA TCAAGAAGGT CTGGAAGAGG 
ACCAGCATGA AGCTCCTGGA CCAGGTCATC CCTCCCATAG GAGATGACGA GGTGACGGTG 
GGGAAGTTCT ACGCCACGTT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAGGCGCCAG 
GAGGAGTATT ATGGGTACCG GCCCAAGAAG GACACCGTGC AGATCCAGGC TGGACTGAGG 
ACCATCGAGG AAGAGGCAGC CCCTGAGATC CGCCGCACCA TCTCGGGAGA CCTGATGGCC 
GAAGAGGAGC TGGAGAGAGC CATGGTGGAG GCCGCGATGG AGGAAGGGAT CTTCCGGAGG 
ACCGGCGGCC TGTTTGGCCA GGTGGACAGC TTCCTGGAAA GGACCAACTC GTTGGCCCCG 
CACATGGCCA ACCAGAGACC CCTCCAGTTT ACTGAAATTG AGATGGAGGA GATGGAATCG 
CCCGTCTTCC TGGAGGACTT CCCTCAACGT CCGAGTGCCC ACCCCCTTGC TCGTGCCAAC 
ACCAACAACG CCAACGCCAA CGTCGCCAGT GGCAACGGCA ACCATGGCAA CAGCCCAGCA 
TTTCCCAGTG TCCACTACGA AGGGGAGCTC GCAGGGGAGA CAGAGACGCC TGCCACCAGA 
GGAGGACTCT TCGGCCAGCC CCGGGGGGCC CTGGGGCCCC ACAGCAAGTC CCGAGTAGAG 
TGGCCGCAGG GACAGCTGCC CCGGAGAAGG ACGCCCAGGG CCCAGGCCCC CGCTGCCTCC 
TGCCAGCACG CGGGAGCAGA GTCCCAGGTG CCCGAGGAGA GGAGCCCCAC CCCAGGGTCC 
CTGCCCGGGG AGGCGCCGCC CGGCAGTAGG ACAGAGGACG GAACCCCTGG GGGTCCAGCT 
CCAGCCATGG CCCTGCTGAT CCGCGAGGCT CTGGTTCGAG GGGGCCTGGA CAGCTTGGCG 
GCGGATGCTA ACTTCATCAT GGCCACGGGC CAGGCGCTGG CAGAGGCCTG CCGGATGGAA 
CCGCGGGAAG TGGAGGTGGC AGCCGCAGAG CTTCTGCAGG GACGCGAGGC CCTGGAGGGC 
GTGCCTGGTG CCCGAGGGTG CCCAGGCCTC GGGTCCTCCC TGGGCAGCCT CGACCTGCGT 
GAGGGCTCCC AGGAGACCCT GATTCCCCGC AGGCCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024832342.1
CDS94..5712
Translation

Target ORF information:

RefSeq Version XM_024976574.1
Organism Bos taurus(cattle)
Definition Bos taurus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024976574.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG 
CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC 
ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC 
AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG 
GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG 
CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC 
GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC 
CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG 
AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG 
TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC 
TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA 
ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC 
TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG 
ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG 
ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG 
CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GGATCTTCAA GATCACCAAG 
TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC 
TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC 
CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG 
TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC 
TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG 
CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG 
GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG 
CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGCGTC CTGTGCCACC GCATTGTCAA TGCCACCTGG 
TTCACTAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCTTGGC CGCCGAGGAC 
CCCCTCCGGG CTGAGTCCGT GAGGAACCAG ATCCTCGGGT ATTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGGGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGG TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTCGAGTC CAGCACCATC TCCGTGGTGA AGATCCTGAG GGTCCTGAGG 
GTGCTCCGAC CGCTCCGGGC CATCAACAGA GCCAAAGGCC TGAAGCACGT GGTACAGTGT 
GTGTTCGTGG CCATCCGCAC CATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCTT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTG 
TCCAAGATGA CGGAGGAGGA GTGCAGGGGC TACTACTATG TGTACAAAGA TGGAGACCCC 
ACACAGATAG AGGTGCGTCC CCGCCAGTGG GTGCACAACG CCTTCCACTT CGACAACGTG 
CTTTCGGCCA TGATGTCCCT CTTCACCGTG TCCACCTTCG AGGGATGGCC TGAGCTGCTG 
TACAAGGCCA TTGACTCTCA TGAGGAGGAC AAGGGCCCCG TGTACAACCA CCGCGTGGAG 
ATGGCCATTT TCTTCATCAT CTACATCATC CTTATCGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTTG TCATCGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTGCAG TACGCCCTGA AGGCCCGGCC CCTGAGGTGC 
TACATCCCCA AGAACCCCTA CCAGTACCGG GTGTGGTACA TCGTCACCTC CTCCTACTTC 
GAGTACCTGA TGTTCGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCGG AGCAAATGAA CCACATCTCT GACATCCTCA ACGTAGCCTT CACCATCATC 
TTCACACTGG AGATGGTCCT CAAGCTCATG GCGTTCAAGG CCAGGGGCTA TTTTGGGGAC 
CCCTGGAACG TGTTTGACTT TCTGATTGTC ATCGGCAGCA TCATCGACGT CATCCTCAGT 
GAGATCGACA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG CGGCTGCGGG 
AACGTTGACC CGGACGAGAG CGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGTGTC 
ATGAGGCTGA TCAAGCTGCT GAGCCGCGCG GAGGGCGTGC GCACGCTGCT CTGGACTTTC 
ATCAAGTCCT TCCAGGCCCT GCCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC 
TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCT TGGTGGACGG GACCCAGATC 
AACCGGAACA ACAACTTCCA GACCTTCCCC CAGGCGGTGC TGCTGCTCTT CAGGTGCGCC 
ACGGGGGAGG CGTGGCAGGA GATCCTGCTG GCCTGCAGCT ACGGCCAGCT GTGTGACCCC 
GAGTCGGACT ACGCCCCCGG GGAGGAGTAC ACCTGTGGCA CGGACTTCGC CTACTACTAC 
TTCCTCAGCT TCTACATGCT GTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCCGTCATC 
ATGGACAACT TTGACTACCT GACACGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT 
GAGTTCAAGG CCATCTGGGC GGAGTACGAC CCGGAGGCTA CGGGCAGGAT CAAGCACCTG 
GACGTGGTGA CCCTGCTGAG GAGGATCCAG CCCCCGCTGG GCTTCGGGAA GTTCTGCCCC 
CACCGGGTAG CGTGTAAGCG GCTGGTGGGC ATGAACATGC CCCTCAACAG TGACGGCACG 
GTCACCTTCA ACGCCACGCT CTTCGCCCTG GTGCGCACAG CTCTGAAGAT CAAGACGGAA 
GGTAACTTCG AGCAGGCCAA CGAGGAGCTG CGAGCCATCA TCAAGAAGGT CTGGAAGAGG 
ACCAGCATGA AGCTCCTGGA CCAGGTCATC CCTCCCATAG GAGATGACGA GGTGACGGTG 
GGGAAGTTCT ACGCCACGTT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAGGCGCCAG 
GAGGAGTATT ATGGGTACCG GCCCAAGAAG GACACCGTGC AGATCCAGGC TGGACTGAGG 
ACCATCGAGG AAGAGGCAGC CCCTGAGATC CGCCGCACCA TCTCGGGAGA CCTGATGGCC 
GAAGAGGAGC TGGAGAGAGC CATGGTGGAG GCCGCGATGG AGGAAGGGAT CTTCCGGAGG 
ACCGGCGGCC TGTTTGGCCA GGTGGACAGC TTCCTGGAAA GGACCAACTC GTTGGCCCCG 
CACATGGCCA ACCAGAGACC CCTCCAGTTT ACTGAAATTG AGATGGAGGA GATGGAATCG 
CCCGTCTTCC TGGAGGACTT CCCTCAACGT CCGAGTGCCC ACCCCCTTGC TCGTGCCAAC 
ACCAACAACG CCAACGCCAA CGTCGCCAGT GGCAACGGCA ACCATGGCAA CAGCCCAGCA 
TTTCCCAGTG TCCACTACGA AGGGGAGCTC GCAGGGGAGA CAGAGACGCC TGCCACCAGA 
GGAGGACTCT TCGGCCAGCC CCGGGGGGCC CTGGGGCCCC ACAGCAAGTC CCGAGTAGAG 
TGGCCGCAGG GACAGCTGCC CCGGAGAAGG ACGCCCAGGG CCCAGGCCCC CGCTGCCTCC 
TGCCAGCACG CGGGAGCAGA GTCCCAGGTG CCCGAGGAGA GGAGCCCCAC CCCAGGGTCC 
CTGCCCGGGG AGGCGCCGCC CGGCAGTAGG ACAGAGGACG GAACCCCTGG GGGTCCAGCT 
CCAGCCATGG CCCTGCTGAT CCGCGAGGCT CTGGTTCGAG GGGGCCTGGA CAGCTTGGCG 
GCGGATGCTA ACTTCATCAT GGCCACGGGC CAGGCGCTGG CAGAGGCCTG CCGGATGGAA 
CCGCGGGAAG TGGAGGTGGC AGCCGCAGAG CTTCTGCAGG GACGCGAGGC CCTGGAGGGC 
GTGCCTGGTG CCCGAGGGTG CCCAGGCCTC GGGTCCTCCC TGGGCAGCCT CGACCTGCGT 
GAGGGCTCCC AGGAGACCCT GATTCCCCGC AGGCCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa223793
Clone ID Related Accession (Same CDS sequence) XM_024976575.1
Accession Version XM_024976575.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5562bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG 
CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC 
ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC 
AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG 
GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG 
CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC 
GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC 
CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG 
AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG 
TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC 
TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA 
ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC 
TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG 
ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG 
ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG 
CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GGATCTTCAA GATCACCAAG 
TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC 
TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC 
CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG 
TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC 
TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG 
CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG 
GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG 
CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGCGTC CTGTGCCACC GCATTGTCAA TGCCACCTGG 
TTCACTAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCTTGGC CGCCGAGGAC 
CCCCTCCGGG CTGAGTCCGT GAGGAACCAG ATCCTCGGGT ATTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGGGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGG TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTCGAGTC CAGCACCATC TCCGTGGTGA AGATCCTGAG GGTCCTGAGG 
GTGCTCCGAC CGCTCCGGGC CATCAACAGA GCCAAAGGCC TGAAGCACGT GGTACAGTGT 
GTGTTCGTGG CCATCCGCAC CATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCTT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTG 
TCCAAGATGA CGGAGGAGGA GTGCAGGGGC TACTACTATG TGTACAAAGA TGGAGACCCC 
ACACAGATAG AGGTGCGTCC CCGCCAGTGG GTGCACAACG CCTTCCACTT CGACAACGTG 
CTTTCGGCCA TGATGTCCCT CTTCACCGTG TCCACCTTCG AGGGATGGCC TGAGCTGCTG 
TACAAGGCCA TTGACTCTCA TGAGGAGGAC AAGGGCCCCG TGTACAACCA CCGCGTGGAG 
ATGGCCATTT TCTTCATCAT CTACATCATC CTTATCGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTTG TCATCGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTGCAG TACGCCCTGA AGGCCCGGCC CCTGAGGTGC 
TACATCCCCA AGAACCCCTA CCAGTACCGG GTGTGGTACA TCGTCACCTC CTCCTACTTC 
GAGTACCTGA TGTTCGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCGG AGCAAATGAA CCACATCTCT GACATCCTCA ACGTAGCCTT CACCATCATC 
TTCACACTGG AGATGGTCCT CAAGCTCATG GCGTTCAAGG CCAGGGGCTA TTTTGGGGAC 
CCCTGGAACG TGTTTGACTT TCTGATTGTC ATCGGCAGCA TCATCGACGT CATCCTCAGT 
GAGATCGACG ACCCGGACGA GAGCGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT 
GTCATGAGGC TGATCAAGCT GCTGAGCCGC GCGGAGGGCG TGCGCACGCT GCTCTGGACT 
TTCATCAAGT CCTTCCAGGC CCTGCCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC 
ATCTACGCGG TCATCGGCAT GCAGATGTTC GGGAAGATCG CCTTGGTGGA CGGGACCCAG 
ATCAACCGGA ACAACAACTT CCAGACCTTC CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC 
GCCACGGGGG AGGCGTGGCA GGAGATCCTG CTGGCCTGCA GCTACGGCCA GCTGTGTGAC 
CCCGAGTCGG ACTACGCCCC CGGGGAGGAG TACACCTGTG GCACGGACTT CGCCTACTAC 
TACTTCCTCA GCTTCTACAT GCTGTGCGCC TTCCTGATCA TCAACCTCTT TGTGGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACACGG GACTGGTCCA TCCTGGGCCC TCATCACCTG 
GATGAGTTCA AGGCCATCTG GGCGGAGTAC GACCCGGAGG CTACGGGCAG GATCAAGCAC 
CTGGACGTGG TGACCCTGCT GAGGAGGATC CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC 
CCCCACCGGG TAGCGTGTAA GCGGCTGGTG GGCATGAACA TGCCCCTCAA CAGTGACGGC 
ACGGTCACCT TCAACGCCAC GCTCTTCGCC CTGGTGCGCA CAGCTCTGAA GATCAAGACG 
GAAGGTAACT TCGAGCAGGC CAACGAGGAG CTGCGAGCCA TCATCAAGAA GGTCTGGAAG 
AGGACCAGCA TGAAGCTCCT GGACCAGGTC ATCCCTCCCA TAGGAGATGA CGAGGTGACG 
GTGGGGAAGT TCTACGCCAC GTTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAGGCGC 
CAGGAGGAGT ATTATGGGTA CCGGCCCAAG AAGGACACCG TGCAGATCCA GGCTGGACTG 
AGGACCATCG AGGAAGAGGC AGCCCCTGAG ATCCGCCGCA CCATCTCGGG AGACCTGATG 
GCCGAAGAGG AGCTGGAGAG AGCCATGGTG GAGGCCGCGA TGGAGGAAGG GATCTTCCGG 
AGGACCGGCG GCCTGTTTGG CCAGGTGGAC AGCTTCCTGG AAAGGACCAA CTCGTTGGCC 
CCGCACATGG CCAACCAGAG ACCCCTCCAG TTTACTGAAA TTGAGATGGA GGAGATGGAA 
TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA CGTCCGAGTG CCCACCCCCT TGCTCGTGCC 
AACACCAACA ACGCCAACGC CAACGTCGCC AGTGGCAACG GCAACCATGG CAACAGCCCA 
GCATTTCCCA GTGTCCACTA CGAAGGGGAG CTCGCAGGGG AGACAGAGAC GCCTGCCACC 
AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG GCCCTGGGGC CCCACAGCAA GTCCCGAGTA 
GAGTGGCCGC AGGGACAGCT GCCCCGGAGA AGGACGCCCA GGGCCCAGGC CCCCGCTGCC 
TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG GTGCCCGAGG AGAGGAGCCC CACCCCAGGG 
TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT AGGACAGAGG ACGGAACCCC TGGGGGTCCA 
GCTCCAGCCA TGGCCCTGCT GATCCGCGAG GCTCTGGTTC GAGGGGGCCT GGACAGCTTG 
GCGGCGGATG CTAACTTCAT CATGGCCACG GGCCAGGCGC TGGCAGAGGC CTGCCGGATG 
GAACCGCGGG AAGTGGAGGT GGCAGCCGCA GAGCTTCTGC AGGGACGCGA GGCCCTGGAG 
GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC CTCGGGTCCT CCCTGGGCAG CCTCGACCTG 
CGTGAGGGCT CCCAGGAGAC CCTGATTCCC CGCAGGCCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024832343.1
CDS94..5655
Translation

Target ORF information:

RefSeq Version XM_024976575.1
Organism Bos taurus(cattle)
Definition Bos taurus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024976575.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG 
CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC 
ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC 
AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG 
GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG 
CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC 
GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC 
CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG 
AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG 
TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC 
TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA 
ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC 
TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG 
ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG 
ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG 
CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GGATCTTCAA GATCACCAAG 
TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC 
TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC 
CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG 
TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC 
TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG 
CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG 
GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG 
CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC 
ATCTTCAGCC CCACCAACAA GATCCGCGTC CTGTGCCACC GCATTGTCAA TGCCACCTGG 
TTCACTAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCTTGGC CGCCGAGGAC 
CCCCTCCGGG CTGAGTCCGT GAGGAACCAG ATCCTCGGGT ATTTTGACAT CGGGTTCACC 
TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGGGCCTT CCTGCACAAG 
GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGG TGGTGGTGGC CGTGTCCCTC 
ATCTCCATGG GACTCGAGTC CAGCACCATC TCCGTGGTGA AGATCCTGAG GGTCCTGAGG 
GTGCTCCGAC CGCTCCGGGC CATCAACAGA GCCAAAGGCC TGAAGCACGT GGTACAGTGT 
GTGTTCGTGG CCATCCGCAC CATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC 
ATGTTCGCTT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTG 
TCCAAGATGA CGGAGGAGGA GTGCAGGGGC TACTACTATG TGTACAAAGA TGGAGACCCC 
ACACAGATAG AGGTGCGTCC CCGCCAGTGG GTGCACAACG CCTTCCACTT CGACAACGTG 
CTTTCGGCCA TGATGTCCCT CTTCACCGTG TCCACCTTCG AGGGATGGCC TGAGCTGCTG 
TACAAGGCCA TTGACTCTCA TGAGGAGGAC AAGGGCCCCG TGTACAACCA CCGCGTGGAG 
ATGGCCATTT TCTTCATCAT CTACATCATC CTTATCGCCT TCTTCATGAT GAACATCTTC 
GTGGGCTTTG TCATCGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG 
CTGGACAAGA ACCAGCGCCA GTGCGTGCAG TACGCCCTGA AGGCCCGGCC CCTGAGGTGC 
TACATCCCCA AGAACCCCTA CCAGTACCGG GTGTGGTACA TCGTCACCTC CTCCTACTTC 
GAGTACCTGA TGTTCGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC 
AACCAGTCGG AGCAAATGAA CCACATCTCT GACATCCTCA ACGTAGCCTT CACCATCATC 
TTCACACTGG AGATGGTCCT CAAGCTCATG GCGTTCAAGG CCAGGGGCTA TTTTGGGGAC 
CCCTGGAACG TGTTTGACTT TCTGATTGTC ATCGGCAGCA TCATCGACGT CATCCTCAGT 
GAGATCGACG ACCCGGACGA GAGCGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT 
GTCATGAGGC TGATCAAGCT GCTGAGCCGC GCGGAGGGCG TGCGCACGCT GCTCTGGACT 
TTCATCAAGT CCTTCCAGGC CCTGCCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC 
ATCTACGCGG TCATCGGCAT GCAGATGTTC GGGAAGATCG CCTTGGTGGA CGGGACCCAG 
ATCAACCGGA ACAACAACTT CCAGACCTTC CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC 
GCCACGGGGG AGGCGTGGCA GGAGATCCTG CTGGCCTGCA GCTACGGCCA GCTGTGTGAC 
CCCGAGTCGG ACTACGCCCC CGGGGAGGAG TACACCTGTG GCACGGACTT CGCCTACTAC 
TACTTCCTCA GCTTCTACAT GCTGTGCGCC TTCCTGATCA TCAACCTCTT TGTGGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACACGG GACTGGTCCA TCCTGGGCCC TCATCACCTG 
GATGAGTTCA AGGCCATCTG GGCGGAGTAC GACCCGGAGG CTACGGGCAG GATCAAGCAC 
CTGGACGTGG TGACCCTGCT GAGGAGGATC CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC 
CCCCACCGGG TAGCGTGTAA GCGGCTGGTG GGCATGAACA TGCCCCTCAA CAGTGACGGC 
ACGGTCACCT TCAACGCCAC GCTCTTCGCC CTGGTGCGCA CAGCTCTGAA GATCAAGACG 
GAAGGTAACT TCGAGCAGGC CAACGAGGAG CTGCGAGCCA TCATCAAGAA GGTCTGGAAG 
AGGACCAGCA TGAAGCTCCT GGACCAGGTC ATCCCTCCCA TAGGAGATGA CGAGGTGACG 
GTGGGGAAGT TCTACGCCAC GTTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAGGCGC 
CAGGAGGAGT ATTATGGGTA CCGGCCCAAG AAGGACACCG TGCAGATCCA GGCTGGACTG 
AGGACCATCG AGGAAGAGGC AGCCCCTGAG ATCCGCCGCA CCATCTCGGG AGACCTGATG 
GCCGAAGAGG AGCTGGAGAG AGCCATGGTG GAGGCCGCGA TGGAGGAAGG GATCTTCCGG 
AGGACCGGCG GCCTGTTTGG CCAGGTGGAC AGCTTCCTGG AAAGGACCAA CTCGTTGGCC 
CCGCACATGG CCAACCAGAG ACCCCTCCAG TTTACTGAAA TTGAGATGGA GGAGATGGAA 
TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA CGTCCGAGTG CCCACCCCCT TGCTCGTGCC 
AACACCAACA ACGCCAACGC CAACGTCGCC AGTGGCAACG GCAACCATGG CAACAGCCCA 
GCATTTCCCA GTGTCCACTA CGAAGGGGAG CTCGCAGGGG AGACAGAGAC GCCTGCCACC 
AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG GCCCTGGGGC CCCACAGCAA GTCCCGAGTA 
GAGTGGCCGC AGGGACAGCT GCCCCGGAGA AGGACGCCCA GGGCCCAGGC CCCCGCTGCC 
TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG GTGCCCGAGG AGAGGAGCCC CACCCCAGGG 
TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT AGGACAGAGG ACGGAACCCC TGGGGGTCCA 
GCTCCAGCCA TGGCCCTGCT GATCCGCGAG GCTCTGGTTC GAGGGGGCCT GGACAGCTTG 
GCGGCGGATG CTAACTTCAT CATGGCCACG GGCCAGGCGC TGGCAGAGGC CTGCCGGATG 
GAACCGCGGG AAGTGGAGGT GGCAGCCGCA GAGCTTCTGC AGGGACGCGA GGCCCTGGAG 
GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC CTCGGGTCCT CCCTGGGCAG CCTCGACCTG 
CGTGAGGGCT CCCAGGAGAC CCTGATTCCC CGCAGGCCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa93088
Clone ID Related Accession (Same CDS sequence) XM_015475322.1 , XM_015466951.1
Accession Version XM_015475322.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5532bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product voltage-dependent L-type calcium channel subunit alpha-1S
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG 
CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC 
ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC 
AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG 
GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG 
CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC 
GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC 
CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG 
AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG 
TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC 
TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA 
ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC 
TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG 
ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG 
ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGTGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG 
CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GCATCTTCAA GATCACCAAG 
TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC 
TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC 
CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG 
TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC 
TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG 
CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG 
GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG 
CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC 
ATCTTCAGCC CCACCAACAA GTGGGTGGCT CCTCGGCGGT GGGGCAGGGT GATGGTGGGG 
TCTCAGAGAA GGCTGCTTCC TGCTCCGAGA AGACCGCCTT CTCCCAACTC TCTGGGCCTA 
CAGTTACACT GTGAGAAAGG CATTCCTTTA ACTTCAGATC TCTTGCTCCA TTTAACGTGC 
CAGCATCCCC AGCAGACGTA TATTTTAAGC CTCACGCGTT ACTTATACCC AAGCGTGACC 
AGATACCTCC TGCTCTGCCA CCAGATCCTC GGGTATTTTG ACATCGGGTT CACCTCTGTC 
TTCACTGTGG AGATTGTCCT CAAGATGACC ACCTACGGGG CCTTCCTGCA CAAGGGCTCC 
TTTTGCCCAA ATGAAACATT CCGTGGGCAC CCAGTGTCTG TCTCCCTCCT TCCCTCCGGG 
ATACAGGGGA AGTTCTTCAG CTGCAATGAC TTGTCCAAGA TGACGGAGGA GGAGTGCAGG 
GGCTACTACT ATGTGTACAA AGATGGAGAC CCCACACAGA TAGAGGTGCG TCCCCGCCAG 
TGGGTGCACA ACGCCTTCCA CTTCGACAAC GTGCTTTCGG CCATGATGTC CCTCTTCACC 
GTGTCCACCT TCGAGGGATG GCCTGAGCTG CTGTACAAGG CCATTGACTC TCATGAGGAG 
GACAAGGGCC CCGTGTACAA CCACCGCGTG GAGATGGCCA TTTTCTTCAT CATCTACATC 
ATCCTTATCG CCTTCTTCAT GATGAACATC TTCGTGGGCT TTGTCATCGT CACCTTCCAG 
GAGCAGGGGG AGACTGAGTA CAAGAACTGT GAGCTGGACA AGAACCAGCG CCAGTGCGTG 
CAGTACGCCC TGAAGGCCCG GCCCCTGAGG TGCTACATCC CCAAGAACCC CTACCAGTAC 
CGGGTGTGGT ACATCGTCAC CTCCTCCTAC TTCGAGTACC TGATGTTCGC CCTCATCATG 
CTCAACACCA TCTGCCTGGG CATGCAGCAC TACAACCAGT CGGAGCAAAT GAACCACATC 
TCTGACATCC TCAACGTAGC CTTCACCATC ATCTTCACAC TGGAGATGGT CCTCAAGCTC 
ATGGCGTTCA AGGCCAGGGG CTATTTTGGG GACCCCTGGA ACGTGTTTGA CTTTCTGATT 
GTCATCGGCA GCATCATCGA CGTCATCCTC AGTGAGATCG ACACTTTCCT GGCCTCCAGC 
GGGGGACTGT ATTGCCTGGG TGGCGGCTGC GGGAACGTTG ACCCGGACGA GAGCGCCCGC 
ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT GTCATGAGGC TGATCAAGCT GCTGAGCCGC 
GCGGAGGGCG TGCGCACGCT GCTCTGGACT TTCCATCCTC GCCCGCAGGC CCTGCCCTAC 
GTGGCTCTGC TCATCGTCAT GCTCTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAGATCG CCTTGGTGGA CGGGACCCAG ATCAACCGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC GCCACGGGGG AGGCGTGGCA GGAGATCCTG 
CTGGCCTGCA GCTACGGCCA GCTGTGTGAC CCCGAGTCGG ACTACGCCCC CGGGGAGGAG 
TACACCTGTG GCACGGACTT CGCCTACTAC TACTTCCTCA GCTTCTACAT GCTGTGCGCC 
TTCCTGATCA TCAACCTCTT TGTGGCCGTC ATCATGGACA ACTTTGACTA CCTGACACGG 
GACTGGTCCA TCCTGGGCCC TCATCACCTG GATGAGTTCA AGGCCATCTG GGCGGAGTAC 
GACCCGGAGG CTACGGGCAG GATCAAGCAC CTGGACGTGG TGACCCTGCT GAGGAGGATC 
CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC CCCCACCGGG TAGCGTGTAA GCGGCTGGTG 
GGCATGAACA TGCCCCTCAA CAGTGACGGC ACGGTCACCT TCAACGCCAC GCTCTTCGCC 
CTGGTGCGCA CAGCTCTGAA GATCAAGACG GAAGGTAACT TCGAGCAGGC CAACGAGGAG 
CTGCGAGCCA TCATCAAGAA GGTCTGGAAG AGGACCAGCA TGAAGCTCCT GGACCAGGTC 
ATCCCTCCCA TAGGAGATGA CGAGGTGACG GTGGGGAAGT TCTACGCCAC GTTCCTCATC 
CAGGAGCACT TCCGGAAGTT CATGAGGCGC CAGGAGGAGT ATTATGGGTA CCGGCCCAAG 
AAGGACACCG TGCAGATCCA GGCTGGACTG AGGACCATCG AGGAAGAGGC AGCCCCTGAG 
ATCCGCCGCA CCATCTCGGG AGACCTGATG GCCGAAGAGG AGCTGGAGAG AGCCATGGTG 
GAGGCCGCGA TGGAGGAAGG GATCTTCCGG AGGACCGGCG GCCTGTTTGG CCAGGTGGAC 
AGCTTCCTGG AAAGGACCAA CTCGTTGGCC CCGCACATGG CCAACCAGAG ACCCCTCCAG 
TTTACTGAAA TTGAGATGGA GGAGATGGAA TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA 
CGTCCGAGTG CCCACCCCCT TGCTCGTGCC AACACCAACA ACGCCAACGC CAACGTCGCC 
AGTGGCAACG GCAACCATGG CAACAGCCCA GCATTTCCCA GTGTCCACTA CGAAGGGGAG 
CTCGCAGGGG AGACAGAGAC GCCTGCCACC AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG 
GCCCTGGGGC CCCACAGCAA GTCCCGAGTA GAGTGGCCGC AGGGACAGCT GCCCCGGAGA 
AGGACGCCCA GGGCCCAGGC CCCCGCTGCC TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG 
GTGCCCGAGG AGAGGAGCCC CACCCCAGGG TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT 
AGGACAGAGG ACGGAACCCC TGGGGGTCCA GCTCCAGCCA TGGCCCTGCT GATCCGCGAG 
GCTCTGGTTC AAGGGGGCCT GGACAGCTTG GCGGCGGATG CTAACTTCAT CATGGCCACG 
GGCCAGGCGC TGGCAGAGGC CTGCCGGATG GAACCGCGGG AAGTGGAGGT GGCAGCCGCA 
GAGCTTCTGC AGGGACGCGA GGCCCTGGAG GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC 
CTCGGGTCCT CCCTGGGCAG CCTCGACCTG CGTGAGGGCT CCCAGGAGAC CCTGATTCCC 
CGCAGGCCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330808.1
CDS95..5626
Translation

Target ORF information:

RefSeq Version XM_015475322.1
Organism Bos taurus(cattle)
Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475322.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG 
CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC 
ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC 
AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG 
GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG 
CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC 
GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC 
CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG 
AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG 
TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC 
TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA 
ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC 
TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG 
ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG 
ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGTGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG 
CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GCATCTTCAA GATCACCAAG 
TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC 
TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC 
CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG 
TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC 
TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG 
CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG 
GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG 
CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC 
ATCTTCAGCC CCACCAACAA GTGGGTGGCT CCTCGGCGGT GGGGCAGGGT GATGGTGGGG 
TCTCAGAGAA GGCTGCTTCC TGCTCCGAGA AGACCGCCTT CTCCCAACTC TCTGGGCCTA 
CAGTTACACT GTGAGAAAGG CATTCCTTTA ACTTCAGATC TCTTGCTCCA TTTAACGTGC 
CAGCATCCCC AGCAGACGTA TATTTTAAGC CTCACGCGTT ACTTATACCC AAGCGTGACC 
AGATACCTCC TGCTCTGCCA CCAGATCCTC GGGTATTTTG ACATCGGGTT CACCTCTGTC 
TTCACTGTGG AGATTGTCCT CAAGATGACC ACCTACGGGG CCTTCCTGCA CAAGGGCTCC 
TTTTGCCCAA ATGAAACATT CCGTGGGCAC CCAGTGTCTG TCTCCCTCCT TCCCTCCGGG 
ATACAGGGGA AGTTCTTCAG CTGCAATGAC TTGTCCAAGA TGACGGAGGA GGAGTGCAGG 
GGCTACTACT ATGTGTACAA AGATGGAGAC CCCACACAGA TAGAGGTGCG TCCCCGCCAG 
TGGGTGCACA ACGCCTTCCA CTTCGACAAC GTGCTTTCGG CCATGATGTC CCTCTTCACC 
GTGTCCACCT TCGAGGGATG GCCTGAGCTG CTGTACAAGG CCATTGACTC TCATGAGGAG 
GACAAGGGCC CCGTGTACAA CCACCGCGTG GAGATGGCCA TTTTCTTCAT CATCTACATC 
ATCCTTATCG CCTTCTTCAT GATGAACATC TTCGTGGGCT TTGTCATCGT CACCTTCCAG 
GAGCAGGGGG AGACTGAGTA CAAGAACTGT GAGCTGGACA AGAACCAGCG CCAGTGCGTG 
CAGTACGCCC TGAAGGCCCG GCCCCTGAGG TGCTACATCC CCAAGAACCC CTACCAGTAC 
CGGGTGTGGT ACATCGTCAC CTCCTCCTAC TTCGAGTACC TGATGTTCGC CCTCATCATG 
CTCAACACCA TCTGCCTGGG CATGCAGCAC TACAACCAGT CGGAGCAAAT GAACCACATC 
TCTGACATCC TCAACGTAGC CTTCACCATC ATCTTCACAC TGGAGATGGT CCTCAAGCTC 
ATGGCGTTCA AGGCCAGGGG CTATTTTGGG GACCCCTGGA ACGTGTTTGA CTTTCTGATT 
GTCATCGGCA GCATCATCGA CGTCATCCTC AGTGAGATCG ACACTTTCCT GGCCTCCAGC 
GGGGGACTGT ATTGCCTGGG TGGCGGCTGC GGGAACGTTG ACCCGGACGA GAGCGCCCGC 
ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT GTCATGAGGC TGATCAAGCT GCTGAGCCGC 
GCGGAGGGCG TGCGCACGCT GCTCTGGACT TTCCATCCTC GCCCGCAGGC CCTGCCCTAC 
GTGGCTCTGC TCATCGTCAT GCTCTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAGATCG CCTTGGTGGA CGGGACCCAG ATCAACCGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC GCCACGGGGG AGGCGTGGCA GGAGATCCTG 
CTGGCCTGCA GCTACGGCCA GCTGTGTGAC CCCGAGTCGG ACTACGCCCC CGGGGAGGAG 
TACACCTGTG GCACGGACTT CGCCTACTAC TACTTCCTCA GCTTCTACAT GCTGTGCGCC 
TTCCTGATCA TCAACCTCTT TGTGGCCGTC ATCATGGACA ACTTTGACTA CCTGACACGG 
GACTGGTCCA TCCTGGGCCC TCATCACCTG GATGAGTTCA AGGCCATCTG GGCGGAGTAC 
GACCCGGAGG CTACGGGCAG GATCAAGCAC CTGGACGTGG TGACCCTGCT GAGGAGGATC 
CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC CCCCACCGGG TAGCGTGTAA GCGGCTGGTG 
GGCATGAACA TGCCCCTCAA CAGTGACGGC ACGGTCACCT TCAACGCCAC GCTCTTCGCC 
CTGGTGCGCA CAGCTCTGAA GATCAAGACG GAAGGTAACT TCGAGCAGGC CAACGAGGAG 
CTGCGAGCCA TCATCAAGAA GGTCTGGAAG AGGACCAGCA TGAAGCTCCT GGACCAGGTC 
ATCCCTCCCA TAGGAGATGA CGAGGTGACG GTGGGGAAGT TCTACGCCAC GTTCCTCATC 
CAGGAGCACT TCCGGAAGTT CATGAGGCGC CAGGAGGAGT ATTATGGGTA CCGGCCCAAG 
AAGGACACCG TGCAGATCCA GGCTGGACTG AGGACCATCG AGGAAGAGGC AGCCCCTGAG 
ATCCGCCGCA CCATCTCGGG AGACCTGATG GCCGAAGAGG AGCTGGAGAG AGCCATGGTG 
GAGGCCGCGA TGGAGGAAGG GATCTTCCGG AGGACCGGCG GCCTGTTTGG CCAGGTGGAC 
AGCTTCCTGG AAAGGACCAA CTCGTTGGCC CCGCACATGG CCAACCAGAG ACCCCTCCAG 
TTTACTGAAA TTGAGATGGA GGAGATGGAA TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA 
CGTCCGAGTG CCCACCCCCT TGCTCGTGCC AACACCAACA ACGCCAACGC CAACGTCGCC 
AGTGGCAACG GCAACCATGG CAACAGCCCA GCATTTCCCA GTGTCCACTA CGAAGGGGAG 
CTCGCAGGGG AGACAGAGAC GCCTGCCACC AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG 
GCCCTGGGGC CCCACAGCAA GTCCCGAGTA GAGTGGCCGC AGGGACAGCT GCCCCGGAGA 
AGGACGCCCA GGGCCCAGGC CCCCGCTGCC TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG 
GTGCCCGAGG AGAGGAGCCC CACCCCAGGG TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT 
AGGACAGAGG ACGGAACCCC TGGGGGTCCA GCTCCAGCCA TGGCCCTGCT GATCCGCGAG 
GCTCTGGTTC AAGGGGGCCT GGACAGCTTG GCGGCGGATG CTAACTTCAT CATGGCCACG 
GGCCAGGCGC TGGCAGAGGC CTGCCGGATG GAACCGCGGG AAGTGGAGGT GGCAGCCGCA 
GAGCTTCTGC AGGGACGCGA GGCCCTGGAG GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC 
CTCGGGTCCT CCCTGGGCAG CCTCGACCTG CGTGAGGGCT CCCAGGAGAC CCTGATTCCC 
CGCAGGCCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa93088
Clone ID Related Accession (Same CDS sequence) XM_015475322.1 , XM_015466951.1
Accession Version XM_015466951.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 5532bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product voltage-dependent L-type calcium channel subunit alpha-1S
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG 
CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC 
ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC 
AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG 
GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG 
CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC 
GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC 
CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG 
AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG 
TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC 
TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA 
ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC 
TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG 
ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG 
ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGTGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG 
CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GCATCTTCAA GATCACCAAG 
TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC 
TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC 
CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG 
TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC 
TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG 
CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG 
GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG 
CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC 
ATCTTCAGCC CCACCAACAA GTGGGTGGCT CCTCGGCGGT GGGGCAGGGT GATGGTGGGG 
TCTCAGAGAA GGCTGCTTCC TGCTCCGAGA AGACCGCCTT CTCCCAACTC TCTGGGCCTA 
CAGTTACACT GTGAGAAAGG CATTCCTTTA ACTTCAGATC TCTTGCTCCA TTTAACGTGC 
CAGCATCCCC AGCAGACGTA TATTTTAAGC CTCACGCGTT ACTTATACCC AAGCGTGACC 
AGATACCTCC TGCTCTGCCA CCAGATCCTC GGGTATTTTG ACATCGGGTT CACCTCTGTC 
TTCACTGTGG AGATTGTCCT CAAGATGACC ACCTACGGGG CCTTCCTGCA CAAGGGCTCC 
TTTTGCCCAA ATGAAACATT CCGTGGGCAC CCAGTGTCTG TCTCCCTCCT TCCCTCCGGG 
ATACAGGGGA AGTTCTTCAG CTGCAATGAC TTGTCCAAGA TGACGGAGGA GGAGTGCAGG 
GGCTACTACT ATGTGTACAA AGATGGAGAC CCCACACAGA TAGAGGTGCG TCCCCGCCAG 
TGGGTGCACA ACGCCTTCCA CTTCGACAAC GTGCTTTCGG CCATGATGTC CCTCTTCACC 
GTGTCCACCT TCGAGGGATG GCCTGAGCTG CTGTACAAGG CCATTGACTC TCATGAGGAG 
GACAAGGGCC CCGTGTACAA CCACCGCGTG GAGATGGCCA TTTTCTTCAT CATCTACATC 
ATCCTTATCG CCTTCTTCAT GATGAACATC TTCGTGGGCT TTGTCATCGT CACCTTCCAG 
GAGCAGGGGG AGACTGAGTA CAAGAACTGT GAGCTGGACA AGAACCAGCG CCAGTGCGTG 
CAGTACGCCC TGAAGGCCCG GCCCCTGAGG TGCTACATCC CCAAGAACCC CTACCAGTAC 
CGGGTGTGGT ACATCGTCAC CTCCTCCTAC TTCGAGTACC TGATGTTCGC CCTCATCATG 
CTCAACACCA TCTGCCTGGG CATGCAGCAC TACAACCAGT CGGAGCAAAT GAACCACATC 
TCTGACATCC TCAACGTAGC CTTCACCATC ATCTTCACAC TGGAGATGGT CCTCAAGCTC 
ATGGCGTTCA AGGCCAGGGG CTATTTTGGG GACCCCTGGA ACGTGTTTGA CTTTCTGATT 
GTCATCGGCA GCATCATCGA CGTCATCCTC AGTGAGATCG ACACTTTCCT GGCCTCCAGC 
GGGGGACTGT ATTGCCTGGG TGGCGGCTGC GGGAACGTTG ACCCGGACGA GAGCGCCCGC 
ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT GTCATGAGGC TGATCAAGCT GCTGAGCCGC 
GCGGAGGGCG TGCGCACGCT GCTCTGGACT TTCCATCCTC GCCCGCAGGC CCTGCCCTAC 
GTGGCTCTGC TCATCGTCAT GCTCTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAGATCG CCTTGGTGGA CGGGACCCAG ATCAACCGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC GCCACGGGGG AGGCGTGGCA GGAGATCCTG 
CTGGCCTGCA GCTACGGCCA GCTGTGTGAC CCCGAGTCGG ACTACGCCCC CGGGGAGGAG 
TACACCTGTG GCACGGACTT CGCCTACTAC TACTTCCTCA GCTTCTACAT GCTGTGCGCC 
TTCCTGATCA TCAACCTCTT TGTGGCCGTC ATCATGGACA ACTTTGACTA CCTGACACGG 
GACTGGTCCA TCCTGGGCCC TCATCACCTG GATGAGTTCA AGGCCATCTG GGCGGAGTAC 
GACCCGGAGG CTACGGGCAG GATCAAGCAC CTGGACGTGG TGACCCTGCT GAGGAGGATC 
CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC CCCCACCGGG TAGCGTGTAA GCGGCTGGTG 
GGCATGAACA TGCCCCTCAA CAGTGACGGC ACGGTCACCT TCAACGCCAC GCTCTTCGCC 
CTGGTGCGCA CAGCTCTGAA GATCAAGACG GAAGGTAACT TCGAGCAGGC CAACGAGGAG 
CTGCGAGCCA TCATCAAGAA GGTCTGGAAG AGGACCAGCA TGAAGCTCCT GGACCAGGTC 
ATCCCTCCCA TAGGAGATGA CGAGGTGACG GTGGGGAAGT TCTACGCCAC GTTCCTCATC 
CAGGAGCACT TCCGGAAGTT CATGAGGCGC CAGGAGGAGT ATTATGGGTA CCGGCCCAAG 
AAGGACACCG TGCAGATCCA GGCTGGACTG AGGACCATCG AGGAAGAGGC AGCCCCTGAG 
ATCCGCCGCA CCATCTCGGG AGACCTGATG GCCGAAGAGG AGCTGGAGAG AGCCATGGTG 
GAGGCCGCGA TGGAGGAAGG GATCTTCCGG AGGACCGGCG GCCTGTTTGG CCAGGTGGAC 
AGCTTCCTGG AAAGGACCAA CTCGTTGGCC CCGCACATGG CCAACCAGAG ACCCCTCCAG 
TTTACTGAAA TTGAGATGGA GGAGATGGAA TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA 
CGTCCGAGTG CCCACCCCCT TGCTCGTGCC AACACCAACA ACGCCAACGC CAACGTCGCC 
AGTGGCAACG GCAACCATGG CAACAGCCCA GCATTTCCCA GTGTCCACTA CGAAGGGGAG 
CTCGCAGGGG AGACAGAGAC GCCTGCCACC AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG 
GCCCTGGGGC CCCACAGCAA GTCCCGAGTA GAGTGGCCGC AGGGACAGCT GCCCCGGAGA 
AGGACGCCCA GGGCCCAGGC CCCCGCTGCC TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG 
GTGCCCGAGG AGAGGAGCCC CACCCCAGGG TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT 
AGGACAGAGG ACGGAACCCC TGGGGGTCCA GCTCCAGCCA TGGCCCTGCT GATCCGCGAG 
GCTCTGGTTC AAGGGGGCCT GGACAGCTTG GCGGCGGATG CTAACTTCAT CATGGCCACG 
GGCCAGGCGC TGGCAGAGGC CTGCCGGATG GAACCGCGGG AAGTGGAGGT GGCAGCCGCA 
GAGCTTCTGC AGGGACGCGA GGCCCTGGAG GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC 
CTCGGGTCCT CCCTGGGCAG CCTCGACCTG CGTGAGGGCT CCCAGGAGAC CCTGATTCCC 
CGCAGGCCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015322437.1
CDS95..5626
Translation

Target ORF information:

RefSeq Version XM_015466951.1
Organism Bos taurus(cattle)
Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015466951.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG 
CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC 
ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC 
AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG 
GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC 
GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG 
CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC 
GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC 
AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC 
CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC 
AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG 
AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG 
TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC 
TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG 
GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG 
CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG 
GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC 
TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA 
ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC 
TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG 
ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG 
ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG 
ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC 
TGCTTCGTGG TGTGCAGTGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG 
CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GCATCTTCAA GATCACCAAG 
TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC 
TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC 
TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC 
CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG 
TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC 
TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT 
GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG 
CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG 
GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG 
AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC 
TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG 
CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC 
ATCTTCAGCC CCACCAACAA GTGGGTGGCT CCTCGGCGGT GGGGCAGGGT GATGGTGGGG 
TCTCAGAGAA GGCTGCTTCC TGCTCCGAGA AGACCGCCTT CTCCCAACTC TCTGGGCCTA 
CAGTTACACT GTGAGAAAGG CATTCCTTTA ACTTCAGATC TCTTGCTCCA TTTAACGTGC 
CAGCATCCCC AGCAGACGTA TATTTTAAGC CTCACGCGTT ACTTATACCC AAGCGTGACC 
AGATACCTCC TGCTCTGCCA CCAGATCCTC GGGTATTTTG ACATCGGGTT CACCTCTGTC 
TTCACTGTGG AGATTGTCCT CAAGATGACC ACCTACGGGG CCTTCCTGCA CAAGGGCTCC 
TTTTGCCCAA ATGAAACATT CCGTGGGCAC CCAGTGTCTG TCTCCCTCCT TCCCTCCGGG 
ATACAGGGGA AGTTCTTCAG CTGCAATGAC TTGTCCAAGA TGACGGAGGA GGAGTGCAGG 
GGCTACTACT ATGTGTACAA AGATGGAGAC CCCACACAGA TAGAGGTGCG TCCCCGCCAG 
TGGGTGCACA ACGCCTTCCA CTTCGACAAC GTGCTTTCGG CCATGATGTC CCTCTTCACC 
GTGTCCACCT TCGAGGGATG GCCTGAGCTG CTGTACAAGG CCATTGACTC TCATGAGGAG 
GACAAGGGCC CCGTGTACAA CCACCGCGTG GAGATGGCCA TTTTCTTCAT CATCTACATC 
ATCCTTATCG CCTTCTTCAT GATGAACATC TTCGTGGGCT TTGTCATCGT CACCTTCCAG 
GAGCAGGGGG AGACTGAGTA CAAGAACTGT GAGCTGGACA AGAACCAGCG CCAGTGCGTG 
CAGTACGCCC TGAAGGCCCG GCCCCTGAGG TGCTACATCC CCAAGAACCC CTACCAGTAC 
CGGGTGTGGT ACATCGTCAC CTCCTCCTAC TTCGAGTACC TGATGTTCGC CCTCATCATG 
CTCAACACCA TCTGCCTGGG CATGCAGCAC TACAACCAGT CGGAGCAAAT GAACCACATC 
TCTGACATCC TCAACGTAGC CTTCACCATC ATCTTCACAC TGGAGATGGT CCTCAAGCTC 
ATGGCGTTCA AGGCCAGGGG CTATTTTGGG GACCCCTGGA ACGTGTTTGA CTTTCTGATT 
GTCATCGGCA GCATCATCGA CGTCATCCTC AGTGAGATCG ACACTTTCCT GGCCTCCAGC 
GGGGGACTGT ATTGCCTGGG TGGCGGCTGC GGGAACGTTG ACCCGGACGA GAGCGCCCGC 
ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT GTCATGAGGC TGATCAAGCT GCTGAGCCGC 
GCGGAGGGCG TGCGCACGCT GCTCTGGACT TTCCATCCTC GCCCGCAGGC CCTGCCCTAC 
GTGGCTCTGC TCATCGTCAT GCTCTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC 
GGGAAGATCG CCTTGGTGGA CGGGACCCAG ATCAACCGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC GCCACGGGGG AGGCGTGGCA GGAGATCCTG 
CTGGCCTGCA GCTACGGCCA GCTGTGTGAC CCCGAGTCGG ACTACGCCCC CGGGGAGGAG 
TACACCTGTG GCACGGACTT CGCCTACTAC TACTTCCTCA GCTTCTACAT GCTGTGCGCC 
TTCCTGATCA TCAACCTCTT TGTGGCCGTC ATCATGGACA ACTTTGACTA CCTGACACGG 
GACTGGTCCA TCCTGGGCCC TCATCACCTG GATGAGTTCA AGGCCATCTG GGCGGAGTAC 
GACCCGGAGG CTACGGGCAG GATCAAGCAC CTGGACGTGG TGACCCTGCT GAGGAGGATC 
CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC CCCCACCGGG TAGCGTGTAA GCGGCTGGTG 
GGCATGAACA TGCCCCTCAA CAGTGACGGC ACGGTCACCT TCAACGCCAC GCTCTTCGCC 
CTGGTGCGCA CAGCTCTGAA GATCAAGACG GAAGGTAACT TCGAGCAGGC CAACGAGGAG 
CTGCGAGCCA TCATCAAGAA GGTCTGGAAG AGGACCAGCA TGAAGCTCCT GGACCAGGTC 
ATCCCTCCCA TAGGAGATGA CGAGGTGACG GTGGGGAAGT TCTACGCCAC GTTCCTCATC 
CAGGAGCACT TCCGGAAGTT CATGAGGCGC CAGGAGGAGT ATTATGGGTA CCGGCCCAAG 
AAGGACACCG TGCAGATCCA GGCTGGACTG AGGACCATCG AGGAAGAGGC AGCCCCTGAG 
ATCCGCCGCA CCATCTCGGG AGACCTGATG GCCGAAGAGG AGCTGGAGAG AGCCATGGTG 
GAGGCCGCGA TGGAGGAAGG GATCTTCCGG AGGACCGGCG GCCTGTTTGG CCAGGTGGAC 
AGCTTCCTGG AAAGGACCAA CTCGTTGGCC CCGCACATGG CCAACCAGAG ACCCCTCCAG 
TTTACTGAAA TTGAGATGGA GGAGATGGAA TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA 
CGTCCGAGTG CCCACCCCCT TGCTCGTGCC AACACCAACA ACGCCAACGC CAACGTCGCC 
AGTGGCAACG GCAACCATGG CAACAGCCCA GCATTTCCCA GTGTCCACTA CGAAGGGGAG 
CTCGCAGGGG AGACAGAGAC GCCTGCCACC AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG 
GCCCTGGGGC CCCACAGCAA GTCCCGAGTA GAGTGGCCGC AGGGACAGCT GCCCCGGAGA 
AGGACGCCCA GGGCCCAGGC CCCCGCTGCC TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG 
GTGCCCGAGG AGAGGAGCCC CACCCCAGGG TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT 
AGGACAGAGG ACGGAACCCC TGGGGGTCCA GCTCCAGCCA TGGCCCTGCT GATCCGCGAG 
GCTCTGGTTC AAGGGGGCCT GGACAGCTTG GCGGCGGATG CTAACTTCAT CATGGCCACG 
GGCCAGGCGC TGGCAGAGGC CTGCCGGATG GAACCGCGGG AAGTGGAGGT GGCAGCCGCA 
GAGCTTCTGC AGGGACGCGA GGCCCTGGAG GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC 
CTCGGGTCCT CCCTGGGCAG CCTCGACCTG CGTGAGGGCT CCCAGGAGAC CCTGATTCCC 
CGCAGGCCAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed