foxi3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	foxi3a
Entrez Gene ID	387257
Full Name	forkhead box I3a
Synonyms	foxi3
General protein information	Preferred Names forkhead box I3a Names forkhead box I3a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_198917.2	NP_944599.2	forkhead box I3a

Pathways from BioSystems

Homologies

	FOXI1	XP_001092246.1
Gallus gallus (chicken)	FOXI1	XP_425185.1
Rattus norvegicus (Norway rat)	Foxi1	NP_001099246.1
Pan troglodytes (chimpanzee)	FOXI1	XP_527110.1
Homo sapiens (human)	FOXI1	NP_036320.2
Canis lupus familiaris (dog)	FOXI1	XP_546245.2
Mus musculus (house mouse)	Foxi1	NP_076396.3
Bos taurus (cattle)	FOXI1	NP_001179172.1
Danio rerio (zebrafish)	foxi3b	NP_944600.1
Danio rerio (zebrafish)	foxi3a	NP_944599.2
Xenopus tropicalis (tropical clawed frog)	foxi1	NP_988949.1

Gene Ontology
Bibliography

Related articles in PubMed

The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models.
Shu Y, Lou Q, Dai Z, Dai X, He J, Hu W, Yin Z
Scientific reports618597(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Stanniocalcin-1 controls ion regulation functions of ion-transporting epithelium other than calcium balance.
Chou MY, Lin CH, Chao PL, Hung JC, Cruz SA, Hwang PP
International journal of biological sciences11(2)122-32(2015)

Zebrafish transforming growth factor-?-stimulated clone 22 domain 3 (TSC22D3) plays critical roles in Bmp-dependent dorsoventral patterning via two deubiquitylating enzymes Usp15 and Otud4.
Tse WK, Jiang YJ, Wong CK
Biochimica et biophysica acta1830(10)4584-93(2013 Oct)

Compensatory regulation of Na+ absorption by Na+/H+ exchanger and Na+-Cl- cotransporter in zebrafish (Danio rerio).
Chang WJ, Wang YF, Hu HJ, Wang JH, Lee TH, Hwang PP
Frontiers in zoology10(1)46(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Treatment of zebrafish embryos with exogenous cortisol significantly increased the density of mature ionocytes and foxi3 expression.
Title: Cortisol promotes differentiation of epidermal ionocytes through Foxi3 transcription factors in zebrafish (Danio rerio).

Data suggest that grhl1 is a novel common marker of all or most "non-keratinocyte" epidermal progenitors, and that the sub-functionalisation of these cells is regulated by differential positive and negative effects of Foxi3 factors.
Title: Zebrafish grainyhead-like1 is a common marker of different non-keratinocyte epidermal cell lineages, which segregate from each other in a Foxi3-dependent manner.

two foxi3 genes are expressed in ionocyte precursors and are required both for ionocyte-specific expression of the Notch ligand Jagged2a, and for ionocyte differentiation, characterized by the production of particular ATPases
Title: Foxi3 transcription factors and Notch signaling control the formation of skin ionocytes from epidermal precursors of the zebrafish embryo.

we propose that foxi3a is a key gene in the control of mitochondria-rich cell differentiation
Title: Visualization in zebrafish larvae of Na(+) uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a.

an extensive phylogenetic analysis of the FoxI class that includes these new sequences together with those previously reported
Title: Expression and phylogenetic analyses of three zebrafish FoxI class genes.

The following foxi3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the foxi3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35907	NM_198917.2 Latest version!	Danio rerio forkhead box I3a (foxi3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

foxi3a ( NM_198917.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_944599.2 Danio rerio forkhead box I3a (foxi3a), mRNA.

Price & Availability↑

CloneID	ODa35907
Clone ID Related Accession (Same CDS sequence)	NM_198917.2
Accession Version	NM_198917.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	forkhead box I3a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133872.1. On Mar 11, 2007 this sequence version replaced NM_198917.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC133872.1, AY331583.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACATCAT TTGTTCCACA GTCTCTTTCA CCTCAGTTCC ACAGCATGGG GCAGGAGTCC 
CAAGAGTTCA GCCTGTACGG TGACAACTTC TACAGCGCCC AGCATGTGCC TAGCCCACAA 
CAAACCCTGC CATCCGCTTA CGATTTCGGA GAATACGCAG GGCAGACCTC CAACCCTTAC 
CTGTGGTTCA ACGGGCCAGG ACTCAGTCCA GCTCCTTGCC TTACCACAGG GCCTCAGCAC 
TATGGGATGG CGAAGCAGTA TGTGGGCGCA AGTGGAATCG GAGGCTCAGA GGGTGCCTTC 
AGCTGGTTCT CTCTGCCATC CCAAGAAGAT CTGATGAAAC TCGTGAGGCC TCCCTACTCG 
TACTCTGCAC TTATTGCGAT GGCAATACAT GGAGCTCCAA ACCGCCGGCT CACCCTCAGC 
CAGATCTACC AGTATGTGGC TGACAACTTT CCTTTCTACA ACAAGAGCAA AGCCAGCTGG 
CAGAACTCAA TCCGGCACAA TCTCTCGCTC AACGACTGCT TCATGAAAGT GCCCAGAGAT 
GACAGCGATC CAGGAAAAGG CAACTATTGG ACTCTTGACC CAAACTGTGA GAAGATGTTT 
GATAATGGAA ACTTCCGGCG CAAGAGGAAG AGAAAGTCGG ACTCTCTGGC TGAAGAGGAG 
GGAAAAGGGT ACTCTGGATC AGACTCTGCT CTGTCCAGTC CTAAAAACCC CAGCGACTCC 
TCGGAGAGAG GAAACTCTCC AATCTCCACA GATCAAGCTC CGTGTCTCAA CAGCTTCCTA 
AATCAAATGG GGGATGTGGC TTCAGGATCC AGAGAGGCTC TCCTCCCTTC TCCTCTGGCT 
GTCCCGCTGA GCCAAAGGTC ATCTCCGACT GGGGTCTATG GCTCATACTC ACCGAACGCA 
ACCATGCCAC AATGGGAAAC CCAAATCCCT CAATCCAGCA TCTCATCCAC TCCCTACAAA 
GATGGCTATA GTGACTCAAT GCTGAACCCG TACAGCAGCC AGCTCTATCC AGTGCTGGGA 
TCCTCTGATT TGCTGTATCC ACGGGAGGGA TCTGAGGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_944599.2
CDS	83..1144
Translation	MTSFVPQSLSPQFHSMGQESQEFSLYGDNFYSAQHVPSPQQTLPSAYDFGEYAGQTSNPYLWFNGPGLSPAPCLTTGPQHYGMAKQYVGASGIGGSEGAFSWFSLPSQEDLMKLVRPPYSYSALIAMAIHGAPNRRLTLSQIYQYVADNFPFYNKSKASWQNSIRHNLSLNDCFMKVPRDDSDPGKGNYWTLDPNCEKMFDNGNFRRKRKRKSDSLAEEEGKGYSGSDSALSSPKNPSDSSERGNSPISTDQAPCLNSFLNQMGDVASGSREALLPSPLAVPLSQRSSPTGVYGSYSPNATMPQWETQIPQSSISSTPYKDGYSDSMLNPYSSQLYPVLGSSDLLYPREGSEV

Target ORF information:

RefSeq Version	NM_198917.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio forkhead box I3a (foxi3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198917.2

ORF Insert Sequence:

ATGACATCAT TTGTTCCACA GTCTCTTTCA CCTCAGTTCC ACAGCATGGG GCAGGAGTCC 
CAAGAGTTCA GCCTGTACGG TGACAACTTC TACAGCGCCC AGCATGTGCC TAGCCCACAA 
CAAACCCTGC CATCCGCTTA CGATTTCGGA GAATACGCAG GGCAGACCTC CAACCCTTAC 
CTGTGGTTCA ACGGGCCAGG ACTCAGTCCA GCTCCTTGCC TTACCACAGG GCCTCAGCAC 
TATGGGATGG CGAAGCAGTA TGTGGGCGCA AGTGGAATCG GAGGCTCAGA GGGTGCCTTC 
AGCTGGTTCT CTCTGCCATC CCAAGAAGAT CTGATGAAAC TCGTGAGGCC TCCCTACTCG 
TACTCTGCAC TTATTGCGAT GGCAATACAT GGAGCTCCAA ACCGCCGGCT CACCCTCAGC 
CAGATCTACC AGTATGTGGC TGACAACTTT CCTTTCTACA ACAAGAGCAA AGCCAGCTGG 
CAGAACTCAA TCCGGCACAA TCTCTCGCTC AACGACTGCT TCATGAAAGT GCCCAGAGAT 
GACAGCGATC CAGGAAAAGG CAACTATTGG ACTCTTGACC CAAACTGTGA GAAGATGTTT 
GATAATGGAA ACTTCCGGCG CAAGAGGAAG AGAAAGTCGG ACTCTCTGGC TGAAGAGGAG 
GGAAAAGGGT ACTCTGGATC AGACTCTGCT CTGTCCAGTC CTAAAAACCC CAGCGACTCC 
TCGGAGAGAG GAAACTCTCC AATCTCCACA GATCAAGCTC CGTGTCTCAA CAGCTTCCTA 
AATCAAATGG GGGATGTGGC TTCAGGATCC AGAGAGGCTC TCCTCCCTTC TCCTCTGGCT 
GTCCCGCTGA GCCAAAGGTC ATCTCCGACT GGGGTCTATG GCTCATACTC ACCGAACGCA 
ACCATGCCAC AATGGGAAAC CCAAATCCCT CAATCCAGCA TCTCATCCAC TCCCTACAAA 
GATGGCTATA GTGACTCAAT GCTGAACCCG TACAGCAGCC AGCTCTATCC AGTGCTGGGA 
TCCTCTGATT TGCTGTATCC ACGGGAGGGA TCTGAGGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models.
Scientific reports618597(2016 Jan)
Shu Y,Lou Q,Dai Z,Dai X,He J,Hu W,Yin Z

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Stanniocalcin-1 controls ion regulation functions of ion-transporting epithelium other than calcium balance.
International journal of biological sciences11(2)122-32(2015)
Chou MY,Lin CH,Chao PL,Hung JC,Cruz SA,Hwang PP

Zebrafish transforming growth factor-?-stimulated clone 22 domain 3 (TSC22D3) plays critical roles in Bmp-dependent dorsoventral patterning via two deubiquitylating enzymes Usp15 and Otud4.
Biochimica et biophysica acta1830(10)4584-93(2013 Oct)
Tse WK,Jiang YJ,Wong CK

Compensatory regulation of Na+ absorption by Na+/H+ exchanger and Na+-Cl- cotransporter in zebrafish (Danio rerio).
Frontiers in zoology10(1)46(2013 Aug)
Chang WJ,Wang YF,Hu HJ,Wang JH,Lee TH,Hwang PP