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IRS1 cDNA ORF clone, Danio rerio(Zebrafish)

Summary

Gene Symbol IRS1
Entrez Gene ID 559281
Full Name insulin receptor substrate 1
Synonyms si:dkeyp-51h1.1,wu:fb51f11,wu:fd14h02
General protein information
Preferred Names

insulin receptor substrate 1

Names

insulin receptor substrate 1
wu:fd14h02
fb51f11
fd14h02
Gene Type protein-coding
Organism Danio rerio(Zebrafish)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_682610.9 XP_687702.4 insulin receptor substrate 1
XM_682610.8 XP_687702.4 insulin receptor substrate 1

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Irs1 NP_037101.1
Gallus gallus (chicken) IRS1 XP_004951158.1
Homo sapiens (human) IRS1 NP_005535.1
Pan troglodytes (chimpanzee) IRS1 XP_001134895.1
Xenopus tropicalis (tropical clawed frog) LOC100495320 XP_004914519.1
Canis lupus familiaris (dog) IRS1 XP_543274.3
Danio rerio (zebrafish) irs1 XP_005157831.1
Bos taurus (cattle) IRS1 XP_003585821.1
Mus musculus (house mouse) Irs1 NP_034700.2

Gene Ontology

The following IRS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa20737 XM_682610.9
Latest version!		 Danio rerio insulin receptor substrate 1 (irs1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa20737 XM_682610.8 Danio rerio insulin receptor substrate 1 (irs1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ODa20737
Clone ID Related Accession (Same CDS sequence) XM_682610.9 , XM_682610.8
Accession Version XM_682610.9 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-06-14
Organism Danio rerio(zebrafish)
Product insulin receptor substrate 1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_682610.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCAAGCC CGACTACGGA GCAAGGATGT TTTTCAGACG TGAAAAAGGT GGGTTATTTA 
AGGAAACCCA AAAGCATGCA CAAAAGGTTT TTTGTACTGA GGGCGGCGAG CGCCTCCGGA 
CCGTCCCGAC TGGAGTACTA TGAAAACGAG AAGAAGTGGA GACACAAGTC TGGAGCGCCG 
AAAAGATCCA TTCCGCTGGA GAGCTGCTTT AACATCAACA AAAGAGCGGA TTCAAAAAAC 
AAACATCTCG TCGCGCTTTA TACCAAGGAT GAGTACTTTG CTATCGCTGC TGACAGCGAG 
ATCGAGCAAG AGTCATGGTA TGAAGCCCTG GTTGATCTCC ATAACAGAGG TAAGGTCCAC 
GAAACTGCCC CGGGCCGTGG AGTTGGCGAG GATAATTACG GGGAAGCCAC GCCGGGACCT 
GCCTTTAAAG AGGTTTGGCA AGTTATATTG AAACCAAAGG GTCTGGGTCA CACAAAGAAC 
TTGATTGGAG TTTACAGATT ATGCCTCACC AATAAGACTA TCAGCTTTGT GAAACTGAAT 
TCGGATGCAG CAGCTGTGGT TTTGCAGTTG ATGAACATTA GGAGATGTGG TCACTCTGAA 
AATTTCTTTT TTATTGAAGT CGGCCGATCT GCTGTGACAG GCCCCGGGGA GTTTTGGATG 
CAAGTGGATG ACTCTGTTGT GGCTCAGAAC ATGCATGAAA CTATATTAGA AGCCATGAAA 
GCCATGAGTG AGGAGTTCAG ACCCCGCAGC AAAAGCCAGT CATCTTCTAA CTGCTCAAAC 
CCCATCTCAG TGCCTGTCAG GCGGCATCAT CACAATAATC CTCCCCCTAG TCAAGTTGGC 
TTAGGCAGGC GCTCTCGGGC GGAGAGTGTG ACGGCCACTT CTCCTGTTAG GCCTGGCAAA 
CACAGCCATT CGTTTAGAGT GAGAGCATCT AGTGATGGAG AGGGCACCAT GTCCAGGCCG 
GCATCTGTGG ACGGGAGTCC AAGCAGCCCG AGCAATGCCC GACCTCAATC GCAACGGCAC 
AGAGGGGGAT CCAGACTTCA CCCGCCACTC AATCACAGCA GATCAATACC CACGCCCACC 
TCACGGTGCT CCCCCTCAGC CATCAGCCCC ATCAGCCTGT CCTCCAGCAG CACGAGCGGG 
CATGGCTCCA CATCTGACTG CCTCTTTCCA CGTCGCTCTA GTGCCTCGAT ATCTGGATCA 
CCCAGCGATG GCGGATTCAT CTCCTCAGAT GAATATGGAT CCAGCCCCTG TGACTTTCGA 
AGCTCGTTTC GTAGCGTGAC TCCAGACTCG CTTGGCCACA CACCACCTGC CAGGGAGGAA 
GAGATAAACA ACTACATCTG CATGGCAAAG CCCGGTTCAC TTCCTGGTGC CGGGAGCCAG 
TCACGAAGCC AATCTAGAGG AACACCGTCA AGACTAGAGG AGCCTGAGTT GGAAAAATGT 
TTTCGGAAAA GAACTCATTC TTCTGGTGCT TCGCCGCCAA CTCCATGCCA TCAGAAAACT 
CCCTCACAAT CGCCTGCTGC ATCTTTAGAG GAATACACTG TGATGATGCC TACATACCCA 
CGAAGCCGCT CTGCATCATC GTCATCATCA ACATATAGGC ACTCATTCAT GCCAACACAT 
TCATACCCAG AGGAAGGTAC AGATGCATCA CAAGCAAAAG ATGAAAGGGG GCCAGATCAC 
AAAGATGACG GCTATATGCC CATGCTGCCA GGCGTTGCGC CTGCAACCCC TACAACCAAA 
AGCAGTGACT ACATGCCCAT GAGTCCAAAA AGTGTGTCTG CACCACAGCA AATTATCAAC 
CCCAGGCAAC ACTCGCATGT CGACTCCAAT GGTTACATGA TGATGTCACC AAGTGGCAGC 
TGCTCTCCAG ATGGCACAAC AAACTATGGC AAGATCTGGA CGAATGGCAT CAACCCTAAG 
CTCTCCGTAG AGAGTATGGA AGGTAAAGTG TCGTCATGTG GAGATTACAT AAACATGTCT 
CCAGCTAGTT GCTCAACAAC AAGTACGCCT CCAGACTGCT TTTTCAACCC TGTGGAAGAT 
CCTCCCAGGC CTATGTATGC TTACTACTCT TTGCCCCGCT CATTCAAACA TGCTAACAGG 
AAGCAGGATC AGAGCCCGCT TCGAGTGTCA CTGGGCTCGA GTCGATTAGC ATACGCTGAC 
TCCTCTTCAT CTTCAGCAAG CAGTGACAGC TTGGGTGGTC AAAGCAATTC CCAGCAGTCT 
GCTGTCAAAC CCAAAAGGTC AGAGGGCAAC GGTAGGCTGA CACGTCCAAC ACGGCTGTCA 
CTGGACGCCA GCAAAGCAAG TACTCTTCCA CGCACACGTG AGAGCCCCTT CCCTCCTGAG 
CCTAAAAGTC CTGGGGAGTA TGTCAACATT GAGTTCAACG ACAAGGCATT TTCAGCGAGC 
TTAGCATCGC TGTTCCCACC TGTTTATTCA GTAAGTGATG CTGAGGCCCC GTCGGATCTT 
TCATCATCTG AGTACATGAA CATGCAGCTG GGATCCCAGG CCAGACGGCT GTGCTCCGAA 
TTGCAGACTT CCTCTTCCAG TTCAGATTAT GCCATAATCA CACCGTCAAA CTCTGTATCC 
TCGCCTCCAC TGCCATGTGA GAGCATATGT GAACGTGACT ACATCAGCAT GCAGTTAGCA 
TCCTGCACTG GCTTTCCTGA CACACAATTG ATGCTAATGA CCGAGGCCCT GCCAGATGCA 
GATGCCCCTC CTTGTTCTGT ATCACCCAAT CATGACGTGA CGACATTAAG CGGCGCTCCT 
GGAAGACCAG CTCTGATTAG CCCTTTATCA GGTCTGAGTG CATTCACACG GGTTAACTCC 
ACTTCCGGAC GAAACCAGGG CGCTAAAGTA ATACGTGCTG ACCCACAGGG TCGACGGCGG 
CACAGTTCAG AGACTTTTGC ATCCACAGCG ACCAGAGGGT CAGTCGACAC ATCGAGCACC 
TGTCAATCAG GGGATGTAAA GCGCCACAGC TCCGCCTCCT TCGAGAATGT GTGGTTGAGA 
CCTGATCAGG CCTCTGCTGC TTCTACCGCT CCATCTGCAT CGACAGTAAC CAACGGACGA 
AGGGAAACCG CTTCAGGCCA AATTCCCACA TTAACAAACC ATGACCAGAA CGGCCTGAAC 
TACATTGATC TGGATCTAAT GCAGAATGGA GAGCAACATG TTCCTGATTG GAATGCTTTC 
CAGTCTCGGC CGGTGGAGCT GGGAGCTGTT GAAGGTATAG AAGAGCTCAG TGCCTATGCC 
AGTATAAACT TCCAGAAATC AGATGAGACC AGAGGGAATC TCACACACAG AGAAGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_687702.4
CDS1007..4306
Translation

Target ORF information:

RefSeq Version XM_682610.9
Organism Danio rerio(zebrafish)
Definition Danio rerio insulin receptor substrate 1 (irs1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_682610.9

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCAAGCC CGACTACGGA GCAAGGATGT TTTTCAGACG TGAAAAAGGT GGGTTATTTA 
AGGAAACCCA AAAGCATGCA CAAAAGGTTT TTTGTACTGA GGGCGGCGAG CGCCTCCGGA 
CCGTCCCGAC TGGAGTACTA TGAAAACGAG AAGAAGTGGA GACACAAGTC TGGAGCGCCG 
AAAAGATCCA TTCCGCTGGA GAGCTGCTTT AACATCAACA AAAGAGCGGA TTCAAAAAAC 
AAACATCTCG TCGCGCTTTA TACCAAGGAT GAGTACTTTG CTATCGCTGC TGACAGCGAG 
ATCGAGCAAG AGTCATGGTA TGAAGCCCTG GTTGATCTCC ATAACAGAGG TAAGGTCCAC 
GAAACTGCCC CGGGCCGTGG AGTTGGCGAG GATAATTACG GGGAAGCCAC GCCGGGACCT 
GCCTTTAAAG AGGTTTGGCA AGTTATATTG AAACCAAAGG GTCTGGGTCA CACAAAGAAC 
TTGATTGGAG TTTACAGATT ATGCCTCACC AATAAGACTA TCAGCTTTGT GAAACTGAAT 
TCGGATGCAG CAGCTGTGGT TTTGCAGTTG ATGAACATTA GGAGATGTGG TCACTCTGAA 
AATTTCTTTT TTATTGAAGT CGGCCGATCT GCTGTGACAG GCCCCGGGGA GTTTTGGATG 
CAAGTGGATG ACTCTGTTGT GGCTCAGAAC ATGCATGAAA CTATATTAGA AGCCATGAAA 
GCCATGAGTG AGGAGTTCAG ACCCCGCAGC AAAAGCCAGT CATCTTCTAA CTGCTCAAAC 
CCCATCTCAG TGCCTGTCAG GCGGCATCAT CACAATAATC CTCCCCCTAG TCAAGTTGGC 
TTAGGCAGGC GCTCTCGGGC GGAGAGTGTG ACGGCCACTT CTCCTGTTAG GCCTGGCAAA 
CACAGCCATT CGTTTAGAGT GAGAGCATCT AGTGATGGAG AGGGCACCAT GTCCAGGCCG 
GCATCTGTGG ACGGGAGTCC AAGCAGCCCG AGCAATGCCC GACCTCAATC GCAACGGCAC 
AGAGGGGGAT CCAGACTTCA CCCGCCACTC AATCACAGCA GATCAATACC CACGCCCACC 
TCACGGTGCT CCCCCTCAGC CATCAGCCCC ATCAGCCTGT CCTCCAGCAG CACGAGCGGG 
CATGGCTCCA CATCTGACTG CCTCTTTCCA CGTCGCTCTA GTGCCTCGAT ATCTGGATCA 
CCCAGCGATG GCGGATTCAT CTCCTCAGAT GAATATGGAT CCAGCCCCTG TGACTTTCGA 
AGCTCGTTTC GTAGCGTGAC TCCAGACTCG CTTGGCCACA CACCACCTGC CAGGGAGGAA 
GAGATAAACA ACTACATCTG CATGGCAAAG CCCGGTTCAC TTCCTGGTGC CGGGAGCCAG 
TCACGAAGCC AATCTAGAGG AACACCGTCA AGACTAGAGG AGCCTGAGTT GGAAAAATGT 
TTTCGGAAAA GAACTCATTC TTCTGGTGCT TCGCCGCCAA CTCCATGCCA TCAGAAAACT 
CCCTCACAAT CGCCTGCTGC ATCTTTAGAG GAATACACTG TGATGATGCC TACATACCCA 
CGAAGCCGCT CTGCATCATC GTCATCATCA ACATATAGGC ACTCATTCAT GCCAACACAT 
TCATACCCAG AGGAAGGTAC AGATGCATCA CAAGCAAAAG ATGAAAGGGG GCCAGATCAC 
AAAGATGACG GCTATATGCC CATGCTGCCA GGCGTTGCGC CTGCAACCCC TACAACCAAA 
AGCAGTGACT ACATGCCCAT GAGTCCAAAA AGTGTGTCTG CACCACAGCA AATTATCAAC 
CCCAGGCAAC ACTCGCATGT CGACTCCAAT GGTTACATGA TGATGTCACC AAGTGGCAGC 
TGCTCTCCAG ATGGCACAAC AAACTATGGC AAGATCTGGA CGAATGGCAT CAACCCTAAG 
CTCTCCGTAG AGAGTATGGA AGGTAAAGTG TCGTCATGTG GAGATTACAT AAACATGTCT 
CCAGCTAGTT GCTCAACAAC AAGTACGCCT CCAGACTGCT TTTTCAACCC TGTGGAAGAT 
CCTCCCAGGC CTATGTATGC TTACTACTCT TTGCCCCGCT CATTCAAACA TGCTAACAGG 
AAGCAGGATC AGAGCCCGCT TCGAGTGTCA CTGGGCTCGA GTCGATTAGC ATACGCTGAC 
TCCTCTTCAT CTTCAGCAAG CAGTGACAGC TTGGGTGGTC AAAGCAATTC CCAGCAGTCT 
GCTGTCAAAC CCAAAAGGTC AGAGGGCAAC GGTAGGCTGA CACGTCCAAC ACGGCTGTCA 
CTGGACGCCA GCAAAGCAAG TACTCTTCCA CGCACACGTG AGAGCCCCTT CCCTCCTGAG 
CCTAAAAGTC CTGGGGAGTA TGTCAACATT GAGTTCAACG ACAAGGCATT TTCAGCGAGC 
TTAGCATCGC TGTTCCCACC TGTTTATTCA GTAAGTGATG CTGAGGCCCC GTCGGATCTT 
TCATCATCTG AGTACATGAA CATGCAGCTG GGATCCCAGG CCAGACGGCT GTGCTCCGAA 
TTGCAGACTT CCTCTTCCAG TTCAGATTAT GCCATAATCA CACCGTCAAA CTCTGTATCC 
TCGCCTCCAC TGCCATGTGA GAGCATATGT GAACGTGACT ACATCAGCAT GCAGTTAGCA 
TCCTGCACTG GCTTTCCTGA CACACAATTG ATGCTAATGA CCGAGGCCCT GCCAGATGCA 
GATGCCCCTC CTTGTTCTGT ATCACCCAAT CATGACGTGA CGACATTAAG CGGCGCTCCT 
GGAAGACCAG CTCTGATTAG CCCTTTATCA GGTCTGAGTG CATTCACACG GGTTAACTCC 
ACTTCCGGAC GAAACCAGGG CGCTAAAGTA ATACGTGCTG ACCCACAGGG TCGACGGCGG 
CACAGTTCAG AGACTTTTGC ATCCACAGCG ACCAGAGGGT CAGTCGACAC ATCGAGCACC 
TGTCAATCAG GGGATGTAAA GCGCCACAGC TCCGCCTCCT TCGAGAATGT GTGGTTGAGA 
CCTGATCAGG CCTCTGCTGC TTCTACCGCT CCATCTGCAT CGACAGTAAC CAACGGACGA 
AGGGAAACCG CTTCAGGCCA AATTCCCACA TTAACAAACC ATGACCAGAA CGGCCTGAAC 
TACATTGATC TGGATCTAAT GCAGAATGGA GAGCAACATG TTCCTGATTG GAATGCTTTC 
CAGTCTCGGC CGGTGGAGCT GGGAGCTGTT GAAGGTATAG AAGAGCTCAG TGCCTATGCC 
AGTATAAACT TCCAGAAATC AGATGAGACC AGAGGGAATC TCACACACAG AGAAGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ODa20737
Clone ID Related Accession (Same CDS sequence) XM_682610.9 , XM_682610.8
Accession Version XM_682610.8 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-23
Organism Danio rerio(Zebrafish)
Product insulin receptor substrate 1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_682610.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCAAGCC CGACTACGGA GCAAGGATGT TTTTCAGACG TGAAAAAGGT GGGTTATTTA 
AGGAAACCCA AAAGCATGCA CAAAAGGTTT TTTGTACTGA GGGCGGCGAG CGCCTCCGGA 
CCGTCCCGAC TGGAGTACTA TGAAAACGAG AAGAAGTGGA GACACAAGTC TGGAGCGCCG 
AAAAGATCCA TTCCGCTGGA GAGCTGCTTT AACATCAACA AAAGAGCGGA TTCAAAAAAC 
AAACATCTCG TCGCGCTTTA TACCAAGGAT GAGTACTTTG CTATCGCTGC TGACAGCGAG 
ATCGAGCAAG AGTCATGGTA TGAAGCCCTG GTTGATCTCC ATAACAGAGG TAAGGTCCAC 
GAAACTGCCC CGGGCCGTGG AGTTGGCGAG GATAATTACG GGGAAGCCAC GCCGGGACCT 
GCCTTTAAAG AGGTTTGGCA AGTTATATTG AAACCAAAGG GTCTGGGTCA CACAAAGAAC 
TTGATTGGAG TTTACAGATT ATGCCTCACC AATAAGACTA TCAGCTTTGT GAAACTGAAT 
TCGGATGCAG CAGCTGTGGT TTTGCAGTTG ATGAACATTA GGAGATGTGG TCACTCTGAA 
AATTTCTTTT TTATTGAAGT CGGCCGATCT GCTGTGACAG GCCCCGGGGA GTTTTGGATG 
CAAGTGGATG ACTCTGTTGT GGCTCAGAAC ATGCATGAAA CTATATTAGA AGCCATGAAA 
GCCATGAGTG AGGAGTTCAG ACCCCGCAGC AAAAGCCAGT CATCTTCTAA CTGCTCAAAC 
CCCATCTCAG TGCCTGTCAG GCGGCATCAT CACAATAATC CTCCCCCTAG TCAAGTTGGC 
TTAGGCAGGC GCTCTCGGGC GGAGAGTGTG ACGGCCACTT CTCCTGTTAG GCCTGGCAAA 
CACAGCCATT CGTTTAGAGT GAGAGCATCT AGTGATGGAG AGGGCACCAT GTCCAGGCCG 
GCATCTGTGG ACGGGAGTCC AAGCAGCCCG AGCAATGCCC GACCTCAATC GCAACGGCAC 
AGAGGGGGAT CCAGACTTCA CCCGCCACTC AATCACAGCA GATCAATACC CACGCCCACC 
TCACGGTGCT CCCCCTCAGC CATCAGCCCC ATCAGCCTGT CCTCCAGCAG CACGAGCGGG 
CATGGCTCCA CATCTGACTG CCTCTTTCCA CGTCGCTCTA GTGCCTCGAT ATCTGGATCA 
CCCAGCGATG GCGGATTCAT CTCCTCAGAT GAATATGGAT CCAGCCCCTG TGACTTTCGA 
AGCTCGTTTC GTAGCGTGAC TCCAGACTCG CTTGGCCACA CACCACCTGC CAGGGAGGAA 
GAGATAAACA ACTACATCTG CATGGCAAAG CCCGGTTCAC TTCCTGGTGC CGGGAGCCAG 
TCACGAAGCC AATCTAGAGG AACACCGTCA AGACTAGAGG AGCCTGAGTT GGAAAAATGT 
TTTCGGAAAA GAACTCATTC TTCTGGTGCT TCGCCGCCAA CTCCATGCCA TCAGAAAACT 
CCCTCACAAT CGCCTGCTGC ATCTTTAGAG GAATACACTG TGATGATGCC TACATACCCA 
CGAAGCCGCT CTGCATCATC GTCATCATCA ACATATAGGC ACTCATTCAT GCCAACACAT 
TCATACCCAG AGGAAGGTAC AGATGCATCA CAAGCAAAAG ATGAAAGGGG GCCAGATCAC 
AAAGATGACG GCTATATGCC CATGCTGCCA GGCGTTGCGC CTGCAACCCC TACAACCAAA 
AGCAGTGACT ACATGCCCAT GAGTCCAAAA AGTGTGTCTG CACCACAGCA AATTATCAAC 
CCCAGGCAAC ACTCGCATGT CGACTCCAAT GGTTACATGA TGATGTCACC AAGTGGCAGC 
TGCTCTCCAG ATGGCACAAC AAACTATGGC AAGATCTGGA CGAATGGCAT CAACCCTAAG 
CTCTCCGTAG AGAGTATGGA AGGTAAAGTG TCGTCATGTG GAGATTACAT AAACATGTCT 
CCAGCTAGTT GCTCAACAAC AAGTACGCCT CCAGACTGCT TTTTCAACCC TGTGGAAGAT 
CCTCCCAGGC CTATGTATGC TTACTACTCT TTGCCCCGCT CATTCAAACA TGCTAACAGG 
AAGCAGGATC AGAGCCCGCT TCGAGTGTCA CTGGGCTCGA GTCGATTAGC ATACGCTGAC 
TCCTCTTCAT CTTCAGCAAG CAGTGACAGC TTGGGTGGTC AAAGCAATTC CCAGCAGTCT 
GCTGTCAAAC CCAAAAGGTC AGAGGGCAAC GGTAGGCTGA CACGTCCAAC ACGGCTGTCA 
CTGGACGCCA GCAAAGCAAG TACTCTTCCA CGCACACGTG AGAGCCCCTT CCCTCCTGAG 
CCTAAAAGTC CTGGGGAGTA TGTCAACATT GAGTTCAACG ACAAGGCATT TTCAGCGAGC 
TTAGCATCGC TGTTCCCACC TGTTTATTCA GTAAGTGATG CTGAGGCCCC GTCGGATCTT 
TCATCATCTG AGTACATGAA CATGCAGCTG GGATCCCAGG CCAGACGGCT GTGCTCCGAA 
TTGCAGACTT CCTCTTCCAG TTCAGATTAT GCCATAATCA CACCGTCAAA CTCTGTATCC 
TCGCCTCCAC TGCCATGTGA GAGCATATGT GAACGTGACT ACATCAGCAT GCAGTTAGCA 
TCCTGCACTG GCTTTCCTGA CACACAATTG ATGCTAATGA CCGAGGCCCT GCCAGATGCA 
GATGCCCCTC CTTGTTCTGT ATCACCCAAT CATGACGTGA CGACATTAAG CGGCGCTCCT 
GGAAGACCAG CTCTGATTAG CCCTTTATCA GGTCTGAGTG CATTCACACG GGTTAACTCC 
ACTTCCGGAC GAAACCAGGG CGCTAAAGTA ATACGTGCTG ACCCACAGGG TCGACGGCGG 
CACAGTTCAG AGACTTTTGC ATCCACAGCG ACCAGAGGGT CAGTCGACAC ATCGAGCACC 
TGTCAATCAG GGGATGTAAA GCGCCACAGC TCCGCCTCCT TCGAGAATGT GTGGTTGAGA 
CCTGATCAGG CCTCTGCTGC TTCTACCGCT CCATCTGCAT CGACAGTAAC CAACGGACGA 
AGGGAAACCG CTTCAGGCCA AATTCCCACA TTAACAAACC ATGACCAGAA CGGCCTGAAC 
TACATTGATC TGGATCTAAT GCAGAATGGA GAGCAACATG TTCCTGATTG GAATGCTTTC 
CAGTCTCGGC CGGTGGAGCT GGGAGCTGTT GAAGGTATAG AAGAGCTCAG TGCCTATGCC 
AGTATAAACT TCCAGAAATC AGATGAGACC AGAGGGAATC TCACACACAG AGAAGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_687702.4
CDS1011..4310
Translation

Target ORF information:

RefSeq Version XM_682610.8
Organism Danio rerio(Zebrafish)
Definition Danio rerio insulin receptor substrate 1 (irs1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_682610.8

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCAAGCC CGACTACGGA GCAAGGATGT TTTTCAGACG TGAAAAAGGT GGGTTATTTA 
AGGAAACCCA AAAGCATGCA CAAAAGGTTT TTTGTACTGA GGGCGGCGAG CGCCTCCGGA 
CCGTCCCGAC TGGAGTACTA TGAAAACGAG AAGAAGTGGA GACACAAGTC TGGAGCGCCG 
AAAAGATCCA TTCCGCTGGA GAGCTGCTTT AACATCAACA AAAGAGCGGA TTCAAAAAAC 
AAACATCTCG TCGCGCTTTA TACCAAGGAT GAGTACTTTG CTATCGCTGC TGACAGCGAG 
ATCGAGCAAG AGTCATGGTA TGAAGCCCTG GTTGATCTCC ATAACAGAGG TAAGGTCCAC 
GAAACTGCCC CGGGCCGTGG AGTTGGCGAG GATAATTACG GGGAAGCCAC GCCGGGACCT 
GCCTTTAAAG AGGTTTGGCA AGTTATATTG AAACCAAAGG GTCTGGGTCA CACAAAGAAC 
TTGATTGGAG TTTACAGATT ATGCCTCACC AATAAGACTA TCAGCTTTGT GAAACTGAAT 
TCGGATGCAG CAGCTGTGGT TTTGCAGTTG ATGAACATTA GGAGATGTGG TCACTCTGAA 
AATTTCTTTT TTATTGAAGT CGGCCGATCT GCTGTGACAG GCCCCGGGGA GTTTTGGATG 
CAAGTGGATG ACTCTGTTGT GGCTCAGAAC ATGCATGAAA CTATATTAGA AGCCATGAAA 
GCCATGAGTG AGGAGTTCAG ACCCCGCAGC AAAAGCCAGT CATCTTCTAA CTGCTCAAAC 
CCCATCTCAG TGCCTGTCAG GCGGCATCAT CACAATAATC CTCCCCCTAG TCAAGTTGGC 
TTAGGCAGGC GCTCTCGGGC GGAGAGTGTG ACGGCCACTT CTCCTGTTAG GCCTGGCAAA 
CACAGCCATT CGTTTAGAGT GAGAGCATCT AGTGATGGAG AGGGCACCAT GTCCAGGCCG 
GCATCTGTGG ACGGGAGTCC AAGCAGCCCG AGCAATGCCC GACCTCAATC GCAACGGCAC 
AGAGGGGGAT CCAGACTTCA CCCGCCACTC AATCACAGCA GATCAATACC CACGCCCACC 
TCACGGTGCT CCCCCTCAGC CATCAGCCCC ATCAGCCTGT CCTCCAGCAG CACGAGCGGG 
CATGGCTCCA CATCTGACTG CCTCTTTCCA CGTCGCTCTA GTGCCTCGAT ATCTGGATCA 
CCCAGCGATG GCGGATTCAT CTCCTCAGAT GAATATGGAT CCAGCCCCTG TGACTTTCGA 
AGCTCGTTTC GTAGCGTGAC TCCAGACTCG CTTGGCCACA CACCACCTGC CAGGGAGGAA 
GAGATAAACA ACTACATCTG CATGGCAAAG CCCGGTTCAC TTCCTGGTGC CGGGAGCCAG 
TCACGAAGCC AATCTAGAGG AACACCGTCA AGACTAGAGG AGCCTGAGTT GGAAAAATGT 
TTTCGGAAAA GAACTCATTC TTCTGGTGCT TCGCCGCCAA CTCCATGCCA TCAGAAAACT 
CCCTCACAAT CGCCTGCTGC ATCTTTAGAG GAATACACTG TGATGATGCC TACATACCCA 
CGAAGCCGCT CTGCATCATC GTCATCATCA ACATATAGGC ACTCATTCAT GCCAACACAT 
TCATACCCAG AGGAAGGTAC AGATGCATCA CAAGCAAAAG ATGAAAGGGG GCCAGATCAC 
AAAGATGACG GCTATATGCC CATGCTGCCA GGCGTTGCGC CTGCAACCCC TACAACCAAA 
AGCAGTGACT ACATGCCCAT GAGTCCAAAA AGTGTGTCTG CACCACAGCA AATTATCAAC 
CCCAGGCAAC ACTCGCATGT CGACTCCAAT GGTTACATGA TGATGTCACC AAGTGGCAGC 
TGCTCTCCAG ATGGCACAAC AAACTATGGC AAGATCTGGA CGAATGGCAT CAACCCTAAG 
CTCTCCGTAG AGAGTATGGA AGGTAAAGTG TCGTCATGTG GAGATTACAT AAACATGTCT 
CCAGCTAGTT GCTCAACAAC AAGTACGCCT CCAGACTGCT TTTTCAACCC TGTGGAAGAT 
CCTCCCAGGC CTATGTATGC TTACTACTCT TTGCCCCGCT CATTCAAACA TGCTAACAGG 
AAGCAGGATC AGAGCCCGCT TCGAGTGTCA CTGGGCTCGA GTCGATTAGC ATACGCTGAC 
TCCTCTTCAT CTTCAGCAAG CAGTGACAGC TTGGGTGGTC AAAGCAATTC CCAGCAGTCT 
GCTGTCAAAC CCAAAAGGTC AGAGGGCAAC GGTAGGCTGA CACGTCCAAC ACGGCTGTCA 
CTGGACGCCA GCAAAGCAAG TACTCTTCCA CGCACACGTG AGAGCCCCTT CCCTCCTGAG 
CCTAAAAGTC CTGGGGAGTA TGTCAACATT GAGTTCAACG ACAAGGCATT TTCAGCGAGC 
TTAGCATCGC TGTTCCCACC TGTTTATTCA GTAAGTGATG CTGAGGCCCC GTCGGATCTT 
TCATCATCTG AGTACATGAA CATGCAGCTG GGATCCCAGG CCAGACGGCT GTGCTCCGAA 
TTGCAGACTT CCTCTTCCAG TTCAGATTAT GCCATAATCA CACCGTCAAA CTCTGTATCC 
TCGCCTCCAC TGCCATGTGA GAGCATATGT GAACGTGACT ACATCAGCAT GCAGTTAGCA 
TCCTGCACTG GCTTTCCTGA CACACAATTG ATGCTAATGA CCGAGGCCCT GCCAGATGCA 
GATGCCCCTC CTTGTTCTGT ATCACCCAAT CATGACGTGA CGACATTAAG CGGCGCTCCT 
GGAAGACCAG CTCTGATTAG CCCTTTATCA GGTCTGAGTG CATTCACACG GGTTAACTCC 
ACTTCCGGAC GAAACCAGGG CGCTAAAGTA ATACGTGCTG ACCCACAGGG TCGACGGCGG 
CACAGTTCAG AGACTTTTGC ATCCACAGCG ACCAGAGGGT CAGTCGACAC ATCGAGCACC 
TGTCAATCAG GGGATGTAAA GCGCCACAGC TCCGCCTCCT TCGAGAATGT GTGGTTGAGA 
CCTGATCAGG CCTCTGCTGC TTCTACCGCT CCATCTGCAT CGACAGTAAC CAACGGACGA 
AGGGAAACCG CTTCAGGCCA AATTCCCACA TTAACAAACC ATGACCAGAA CGGCCTGAAC 
TACATTGATC TGGATCTAAT GCAGAATGGA GAGCAACATG TTCCTGATTG GAATGCTTTC 
CAGTCTCGGC CGGTGGAGCT GGGAGCTGTT GAAGGTATAG AAGAGCTCAG TGCCTATGCC 
AGTATAAACT TCCAGAAATC AGATGAGACC AGAGGGAATC TCACACACAG AGAAGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed