ORF » Species Summary » Danio rerio » IRS1 cDNA ORF clone

IRS1 cDNA ORF clone, Danio rerio(Zebrafish)

The following IRS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa20737 XM_682610.9
Latest version!		 Danio rerio insulin receptor substrate 1 (irs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa20737 XM_682610.8 Danio rerio insulin receptor substrate 1 (irs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    IRS1 ( XM_682610.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_687702.4 Danio rerio insulin receptor substrate 1 (irs1), mRNA.
    Price & Availability↑
    CloneID ODa20737
    Clone ID Related Accession (Same CDS sequence) XM_682610.9 , XM_682610.8
    Accession Version XM_682610.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product insulin receptor substrate 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_682610.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCAAGCC CGACTACGGA GCAAGGATGT TTTTCAGACG TGAAAAAGGT GGGTTATTTA 
    AGGAAACCCA AAAGCATGCA CAAAAGGTTT TTTGTACTGA GGGCGGCGAG CGCCTCCGGA 
    CCGTCCCGAC TGGAGTACTA TGAAAACGAG AAGAAGTGGA GACACAAGTC TGGAGCGCCG 
    AAAAGATCCA TTCCGCTGGA GAGCTGCTTT AACATCAACA AAAGAGCGGA TTCAAAAAAC 
    AAACATCTCG TCGCGCTTTA TACCAAGGAT GAGTACTTTG CTATCGCTGC TGACAGCGAG 
    ATCGAGCAAG AGTCATGGTA TGAAGCCCTG GTTGATCTCC ATAACAGAGG TAAGGTCCAC 
    GAAACTGCCC CGGGCCGTGG AGTTGGCGAG GATAATTACG GGGAAGCCAC GCCGGGACCT 
    GCCTTTAAAG AGGTTTGGCA AGTTATATTG AAACCAAAGG GTCTGGGTCA CACAAAGAAC 
    TTGATTGGAG TTTACAGATT ATGCCTCACC AATAAGACTA TCAGCTTTGT GAAACTGAAT 
    TCGGATGCAG CAGCTGTGGT TTTGCAGTTG ATGAACATTA GGAGATGTGG TCACTCTGAA 
    AATTTCTTTT TTATTGAAGT CGGCCGATCT GCTGTGACAG GCCCCGGGGA GTTTTGGATG 
    CAAGTGGATG ACTCTGTTGT GGCTCAGAAC ATGCATGAAA CTATATTAGA AGCCATGAAA 
    GCCATGAGTG AGGAGTTCAG ACCCCGCAGC AAAAGCCAGT CATCTTCTAA CTGCTCAAAC 
    CCCATCTCAG TGCCTGTCAG GCGGCATCAT CACAATAATC CTCCCCCTAG TCAAGTTGGC 
    TTAGGCAGGC GCTCTCGGGC GGAGAGTGTG ACGGCCACTT CTCCTGTTAG GCCTGGCAAA 
    CACAGCCATT CGTTTAGAGT GAGAGCATCT AGTGATGGAG AGGGCACCAT GTCCAGGCCG 
    GCATCTGTGG ACGGGAGTCC AAGCAGCCCG AGCAATGCCC GACCTCAATC GCAACGGCAC 
    AGAGGGGGAT CCAGACTTCA CCCGCCACTC AATCACAGCA GATCAATACC CACGCCCACC 
    TCACGGTGCT CCCCCTCAGC CATCAGCCCC ATCAGCCTGT CCTCCAGCAG CACGAGCGGG 
    CATGGCTCCA CATCTGACTG CCTCTTTCCA CGTCGCTCTA GTGCCTCGAT ATCTGGATCA 
    CCCAGCGATG GCGGATTCAT CTCCTCAGAT GAATATGGAT CCAGCCCCTG TGACTTTCGA 
    AGCTCGTTTC GTAGCGTGAC TCCAGACTCG CTTGGCCACA CACCACCTGC CAGGGAGGAA 
    GAGATAAACA ACTACATCTG CATGGCAAAG CCCGGTTCAC TTCCTGGTGC CGGGAGCCAG 
    TCACGAAGCC AATCTAGAGG AACACCGTCA AGACTAGAGG AGCCTGAGTT GGAAAAATGT 
    TTTCGGAAAA GAACTCATTC TTCTGGTGCT TCGCCGCCAA CTCCATGCCA TCAGAAAACT 
    CCCTCACAAT CGCCTGCTGC ATCTTTAGAG GAATACACTG TGATGATGCC TACATACCCA 
    CGAAGCCGCT CTGCATCATC GTCATCATCA ACATATAGGC ACTCATTCAT GCCAACACAT 
    TCATACCCAG AGGAAGGTAC AGATGCATCA CAAGCAAAAG ATGAAAGGGG GCCAGATCAC 
    AAAGATGACG GCTATATGCC CATGCTGCCA GGCGTTGCGC CTGCAACCCC TACAACCAAA 
    AGCAGTGACT ACATGCCCAT GAGTCCAAAA AGTGTGTCTG CACCACAGCA AATTATCAAC 
    CCCAGGCAAC ACTCGCATGT CGACTCCAAT GGTTACATGA TGATGTCACC AAGTGGCAGC 
    TGCTCTCCAG ATGGCACAAC AAACTATGGC AAGATCTGGA CGAATGGCAT CAACCCTAAG 
    CTCTCCGTAG AGAGTATGGA AGGTAAAGTG TCGTCATGTG GAGATTACAT AAACATGTCT 
    CCAGCTAGTT GCTCAACAAC AAGTACGCCT CCAGACTGCT TTTTCAACCC TGTGGAAGAT 
    CCTCCCAGGC CTATGTATGC TTACTACTCT TTGCCCCGCT CATTCAAACA TGCTAACAGG 
    AAGCAGGATC AGAGCCCGCT TCGAGTGTCA CTGGGCTCGA GTCGATTAGC ATACGCTGAC 
    TCCTCTTCAT CTTCAGCAAG CAGTGACAGC TTGGGTGGTC AAAGCAATTC CCAGCAGTCT 
    GCTGTCAAAC CCAAAAGGTC AGAGGGCAAC GGTAGGCTGA CACGTCCAAC ACGGCTGTCA 
    CTGGACGCCA GCAAAGCAAG TACTCTTCCA CGCACACGTG AGAGCCCCTT CCCTCCTGAG 
    CCTAAAAGTC CTGGGGAGTA TGTCAACATT GAGTTCAACG ACAAGGCATT TTCAGCGAGC 
    TTAGCATCGC TGTTCCCACC TGTTTATTCA GTAAGTGATG CTGAGGCCCC GTCGGATCTT 
    TCATCATCTG AGTACATGAA CATGCAGCTG GGATCCCAGG CCAGACGGCT GTGCTCCGAA 
    TTGCAGACTT CCTCTTCCAG TTCAGATTAT GCCATAATCA CACCGTCAAA CTCTGTATCC 
    TCGCCTCCAC TGCCATGTGA GAGCATATGT GAACGTGACT ACATCAGCAT GCAGTTAGCA 
    TCCTGCACTG GCTTTCCTGA CACACAATTG ATGCTAATGA CCGAGGCCCT GCCAGATGCA 
    GATGCCCCTC CTTGTTCTGT ATCACCCAAT CATGACGTGA CGACATTAAG CGGCGCTCCT 
    GGAAGACCAG CTCTGATTAG CCCTTTATCA GGTCTGAGTG CATTCACACG GGTTAACTCC 
    ACTTCCGGAC GAAACCAGGG CGCTAAAGTA ATACGTGCTG ACCCACAGGG TCGACGGCGG 
    CACAGTTCAG AGACTTTTGC ATCCACAGCG ACCAGAGGGT CAGTCGACAC ATCGAGCACC 
    TGTCAATCAG GGGATGTAAA GCGCCACAGC TCCGCCTCCT TCGAGAATGT GTGGTTGAGA 
    CCTGATCAGG CCTCTGCTGC TTCTACCGCT CCATCTGCAT CGACAGTAAC CAACGGACGA 
    AGGGAAACCG CTTCAGGCCA AATTCCCACA TTAACAAACC ATGACCAGAA CGGCCTGAAC 
    TACATTGATC TGGATCTAAT GCAGAATGGA GAGCAACATG TTCCTGATTG GAATGCTTTC 
    CAGTCTCGGC CGGTGGAGCT GGGAGCTGTT GAAGGTATAG AAGAGCTCAG TGCCTATGCC 
    AGTATAAACT TCCAGAAATC AGATGAGACC AGAGGGAATC TCACACACAG AGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687702.4
    CDS1007..4306
    Translation

    Target ORF information:

    RefSeq Version XM_682610.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio insulin receptor substrate 1 (irs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_682610.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCAAGCC CGACTACGGA GCAAGGATGT TTTTCAGACG TGAAAAAGGT GGGTTATTTA 
    AGGAAACCCA AAAGCATGCA CAAAAGGTTT TTTGTACTGA GGGCGGCGAG CGCCTCCGGA 
    CCGTCCCGAC TGGAGTACTA TGAAAACGAG AAGAAGTGGA GACACAAGTC TGGAGCGCCG 
    AAAAGATCCA TTCCGCTGGA GAGCTGCTTT AACATCAACA AAAGAGCGGA TTCAAAAAAC 
    AAACATCTCG TCGCGCTTTA TACCAAGGAT GAGTACTTTG CTATCGCTGC TGACAGCGAG 
    ATCGAGCAAG AGTCATGGTA TGAAGCCCTG GTTGATCTCC ATAACAGAGG TAAGGTCCAC 
    GAAACTGCCC CGGGCCGTGG AGTTGGCGAG GATAATTACG GGGAAGCCAC GCCGGGACCT 
    GCCTTTAAAG AGGTTTGGCA AGTTATATTG AAACCAAAGG GTCTGGGTCA CACAAAGAAC 
    TTGATTGGAG TTTACAGATT ATGCCTCACC AATAAGACTA TCAGCTTTGT GAAACTGAAT 
    TCGGATGCAG CAGCTGTGGT TTTGCAGTTG ATGAACATTA GGAGATGTGG TCACTCTGAA 
    AATTTCTTTT TTATTGAAGT CGGCCGATCT GCTGTGACAG GCCCCGGGGA GTTTTGGATG 
    CAAGTGGATG ACTCTGTTGT GGCTCAGAAC ATGCATGAAA CTATATTAGA AGCCATGAAA 
    GCCATGAGTG AGGAGTTCAG ACCCCGCAGC AAAAGCCAGT CATCTTCTAA CTGCTCAAAC 
    CCCATCTCAG TGCCTGTCAG GCGGCATCAT CACAATAATC CTCCCCCTAG TCAAGTTGGC 
    TTAGGCAGGC GCTCTCGGGC GGAGAGTGTG ACGGCCACTT CTCCTGTTAG GCCTGGCAAA 
    CACAGCCATT CGTTTAGAGT GAGAGCATCT AGTGATGGAG AGGGCACCAT GTCCAGGCCG 
    GCATCTGTGG ACGGGAGTCC AAGCAGCCCG AGCAATGCCC GACCTCAATC GCAACGGCAC 
    AGAGGGGGAT CCAGACTTCA CCCGCCACTC AATCACAGCA GATCAATACC CACGCCCACC 
    TCACGGTGCT CCCCCTCAGC CATCAGCCCC ATCAGCCTGT CCTCCAGCAG CACGAGCGGG 
    CATGGCTCCA CATCTGACTG CCTCTTTCCA CGTCGCTCTA GTGCCTCGAT ATCTGGATCA 
    CCCAGCGATG GCGGATTCAT CTCCTCAGAT GAATATGGAT CCAGCCCCTG TGACTTTCGA 
    AGCTCGTTTC GTAGCGTGAC TCCAGACTCG CTTGGCCACA CACCACCTGC CAGGGAGGAA 
    GAGATAAACA ACTACATCTG CATGGCAAAG CCCGGTTCAC TTCCTGGTGC CGGGAGCCAG 
    TCACGAAGCC AATCTAGAGG AACACCGTCA AGACTAGAGG AGCCTGAGTT GGAAAAATGT 
    TTTCGGAAAA GAACTCATTC TTCTGGTGCT TCGCCGCCAA CTCCATGCCA TCAGAAAACT 
    CCCTCACAAT CGCCTGCTGC ATCTTTAGAG GAATACACTG TGATGATGCC TACATACCCA 
    CGAAGCCGCT CTGCATCATC GTCATCATCA ACATATAGGC ACTCATTCAT GCCAACACAT 
    TCATACCCAG AGGAAGGTAC AGATGCATCA CAAGCAAAAG ATGAAAGGGG GCCAGATCAC 
    AAAGATGACG GCTATATGCC CATGCTGCCA GGCGTTGCGC CTGCAACCCC TACAACCAAA 
    AGCAGTGACT ACATGCCCAT GAGTCCAAAA AGTGTGTCTG CACCACAGCA AATTATCAAC 
    CCCAGGCAAC ACTCGCATGT CGACTCCAAT GGTTACATGA TGATGTCACC AAGTGGCAGC 
    TGCTCTCCAG ATGGCACAAC AAACTATGGC AAGATCTGGA CGAATGGCAT CAACCCTAAG 
    CTCTCCGTAG AGAGTATGGA AGGTAAAGTG TCGTCATGTG GAGATTACAT AAACATGTCT 
    CCAGCTAGTT GCTCAACAAC AAGTACGCCT CCAGACTGCT TTTTCAACCC TGTGGAAGAT 
    CCTCCCAGGC CTATGTATGC TTACTACTCT TTGCCCCGCT CATTCAAACA TGCTAACAGG 
    AAGCAGGATC AGAGCCCGCT TCGAGTGTCA CTGGGCTCGA GTCGATTAGC ATACGCTGAC 
    TCCTCTTCAT CTTCAGCAAG CAGTGACAGC TTGGGTGGTC AAAGCAATTC CCAGCAGTCT 
    GCTGTCAAAC CCAAAAGGTC AGAGGGCAAC GGTAGGCTGA CACGTCCAAC ACGGCTGTCA 
    CTGGACGCCA GCAAAGCAAG TACTCTTCCA CGCACACGTG AGAGCCCCTT CCCTCCTGAG 
    CCTAAAAGTC CTGGGGAGTA TGTCAACATT GAGTTCAACG ACAAGGCATT TTCAGCGAGC 
    TTAGCATCGC TGTTCCCACC TGTTTATTCA GTAAGTGATG CTGAGGCCCC GTCGGATCTT 
    TCATCATCTG AGTACATGAA CATGCAGCTG GGATCCCAGG CCAGACGGCT GTGCTCCGAA 
    TTGCAGACTT CCTCTTCCAG TTCAGATTAT GCCATAATCA CACCGTCAAA CTCTGTATCC 
    TCGCCTCCAC TGCCATGTGA GAGCATATGT GAACGTGACT ACATCAGCAT GCAGTTAGCA 
    TCCTGCACTG GCTTTCCTGA CACACAATTG ATGCTAATGA CCGAGGCCCT GCCAGATGCA 
    GATGCCCCTC CTTGTTCTGT ATCACCCAAT CATGACGTGA CGACATTAAG CGGCGCTCCT 
    GGAAGACCAG CTCTGATTAG CCCTTTATCA GGTCTGAGTG CATTCACACG GGTTAACTCC 
    ACTTCCGGAC GAAACCAGGG CGCTAAAGTA ATACGTGCTG ACCCACAGGG TCGACGGCGG 
    CACAGTTCAG AGACTTTTGC ATCCACAGCG ACCAGAGGGT CAGTCGACAC ATCGAGCACC 
    TGTCAATCAG GGGATGTAAA GCGCCACAGC TCCGCCTCCT TCGAGAATGT GTGGTTGAGA 
    CCTGATCAGG CCTCTGCTGC TTCTACCGCT CCATCTGCAT CGACAGTAAC CAACGGACGA 
    AGGGAAACCG CTTCAGGCCA AATTCCCACA TTAACAAACC ATGACCAGAA CGGCCTGAAC 
    TACATTGATC TGGATCTAAT GCAGAATGGA GAGCAACATG TTCCTGATTG GAATGCTTTC 
    CAGTCTCGGC CGGTGGAGCT GGGAGCTGTT GAAGGTATAG AAGAGCTCAG TGCCTATGCC 
    AGTATAAACT TCCAGAAATC AGATGAGACC AGAGGGAATC TCACACACAG AGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    IRS1 ( XM_682610.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_687702.4 Danio rerio insulin receptor substrate 1 (irs1), mRNA.
    Price & Availability↑
    CloneID ODa20737
    Clone ID Related Accession (Same CDS sequence) XM_682610.9 , XM_682610.8
    Accession Version XM_682610.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product insulin receptor substrate 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_682610.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCAAGCC CGACTACGGA GCAAGGATGT TTTTCAGACG TGAAAAAGGT GGGTTATTTA 
    AGGAAACCCA AAAGCATGCA CAAAAGGTTT TTTGTACTGA GGGCGGCGAG CGCCTCCGGA 
    CCGTCCCGAC TGGAGTACTA TGAAAACGAG AAGAAGTGGA GACACAAGTC TGGAGCGCCG 
    AAAAGATCCA TTCCGCTGGA GAGCTGCTTT AACATCAACA AAAGAGCGGA TTCAAAAAAC 
    AAACATCTCG TCGCGCTTTA TACCAAGGAT GAGTACTTTG CTATCGCTGC TGACAGCGAG 
    ATCGAGCAAG AGTCATGGTA TGAAGCCCTG GTTGATCTCC ATAACAGAGG TAAGGTCCAC 
    GAAACTGCCC CGGGCCGTGG AGTTGGCGAG GATAATTACG GGGAAGCCAC GCCGGGACCT 
    GCCTTTAAAG AGGTTTGGCA AGTTATATTG AAACCAAAGG GTCTGGGTCA CACAAAGAAC 
    TTGATTGGAG TTTACAGATT ATGCCTCACC AATAAGACTA TCAGCTTTGT GAAACTGAAT 
    TCGGATGCAG CAGCTGTGGT TTTGCAGTTG ATGAACATTA GGAGATGTGG TCACTCTGAA 
    AATTTCTTTT TTATTGAAGT CGGCCGATCT GCTGTGACAG GCCCCGGGGA GTTTTGGATG 
    CAAGTGGATG ACTCTGTTGT GGCTCAGAAC ATGCATGAAA CTATATTAGA AGCCATGAAA 
    GCCATGAGTG AGGAGTTCAG ACCCCGCAGC AAAAGCCAGT CATCTTCTAA CTGCTCAAAC 
    CCCATCTCAG TGCCTGTCAG GCGGCATCAT CACAATAATC CTCCCCCTAG TCAAGTTGGC 
    TTAGGCAGGC GCTCTCGGGC GGAGAGTGTG ACGGCCACTT CTCCTGTTAG GCCTGGCAAA 
    CACAGCCATT CGTTTAGAGT GAGAGCATCT AGTGATGGAG AGGGCACCAT GTCCAGGCCG 
    GCATCTGTGG ACGGGAGTCC AAGCAGCCCG AGCAATGCCC GACCTCAATC GCAACGGCAC 
    AGAGGGGGAT CCAGACTTCA CCCGCCACTC AATCACAGCA GATCAATACC CACGCCCACC 
    TCACGGTGCT CCCCCTCAGC CATCAGCCCC ATCAGCCTGT CCTCCAGCAG CACGAGCGGG 
    CATGGCTCCA CATCTGACTG CCTCTTTCCA CGTCGCTCTA GTGCCTCGAT ATCTGGATCA 
    CCCAGCGATG GCGGATTCAT CTCCTCAGAT GAATATGGAT CCAGCCCCTG TGACTTTCGA 
    AGCTCGTTTC GTAGCGTGAC TCCAGACTCG CTTGGCCACA CACCACCTGC CAGGGAGGAA 
    GAGATAAACA ACTACATCTG CATGGCAAAG CCCGGTTCAC TTCCTGGTGC CGGGAGCCAG 
    TCACGAAGCC AATCTAGAGG AACACCGTCA AGACTAGAGG AGCCTGAGTT GGAAAAATGT 
    TTTCGGAAAA GAACTCATTC TTCTGGTGCT TCGCCGCCAA CTCCATGCCA TCAGAAAACT 
    CCCTCACAAT CGCCTGCTGC ATCTTTAGAG GAATACACTG TGATGATGCC TACATACCCA 
    CGAAGCCGCT CTGCATCATC GTCATCATCA ACATATAGGC ACTCATTCAT GCCAACACAT 
    TCATACCCAG AGGAAGGTAC AGATGCATCA CAAGCAAAAG ATGAAAGGGG GCCAGATCAC 
    AAAGATGACG GCTATATGCC CATGCTGCCA GGCGTTGCGC CTGCAACCCC TACAACCAAA 
    AGCAGTGACT ACATGCCCAT GAGTCCAAAA AGTGTGTCTG CACCACAGCA AATTATCAAC 
    CCCAGGCAAC ACTCGCATGT CGACTCCAAT GGTTACATGA TGATGTCACC AAGTGGCAGC 
    TGCTCTCCAG ATGGCACAAC AAACTATGGC AAGATCTGGA CGAATGGCAT CAACCCTAAG 
    CTCTCCGTAG AGAGTATGGA AGGTAAAGTG TCGTCATGTG GAGATTACAT AAACATGTCT 
    CCAGCTAGTT GCTCAACAAC AAGTACGCCT CCAGACTGCT TTTTCAACCC TGTGGAAGAT 
    CCTCCCAGGC CTATGTATGC TTACTACTCT TTGCCCCGCT CATTCAAACA TGCTAACAGG 
    AAGCAGGATC AGAGCCCGCT TCGAGTGTCA CTGGGCTCGA GTCGATTAGC ATACGCTGAC 
    TCCTCTTCAT CTTCAGCAAG CAGTGACAGC TTGGGTGGTC AAAGCAATTC CCAGCAGTCT 
    GCTGTCAAAC CCAAAAGGTC AGAGGGCAAC GGTAGGCTGA CACGTCCAAC ACGGCTGTCA 
    CTGGACGCCA GCAAAGCAAG TACTCTTCCA CGCACACGTG AGAGCCCCTT CCCTCCTGAG 
    CCTAAAAGTC CTGGGGAGTA TGTCAACATT GAGTTCAACG ACAAGGCATT TTCAGCGAGC 
    TTAGCATCGC TGTTCCCACC TGTTTATTCA GTAAGTGATG CTGAGGCCCC GTCGGATCTT 
    TCATCATCTG AGTACATGAA CATGCAGCTG GGATCCCAGG CCAGACGGCT GTGCTCCGAA 
    TTGCAGACTT CCTCTTCCAG TTCAGATTAT GCCATAATCA CACCGTCAAA CTCTGTATCC 
    TCGCCTCCAC TGCCATGTGA GAGCATATGT GAACGTGACT ACATCAGCAT GCAGTTAGCA 
    TCCTGCACTG GCTTTCCTGA CACACAATTG ATGCTAATGA CCGAGGCCCT GCCAGATGCA 
    GATGCCCCTC CTTGTTCTGT ATCACCCAAT CATGACGTGA CGACATTAAG CGGCGCTCCT 
    GGAAGACCAG CTCTGATTAG CCCTTTATCA GGTCTGAGTG CATTCACACG GGTTAACTCC 
    ACTTCCGGAC GAAACCAGGG CGCTAAAGTA ATACGTGCTG ACCCACAGGG TCGACGGCGG 
    CACAGTTCAG AGACTTTTGC ATCCACAGCG ACCAGAGGGT CAGTCGACAC ATCGAGCACC 
    TGTCAATCAG GGGATGTAAA GCGCCACAGC TCCGCCTCCT TCGAGAATGT GTGGTTGAGA 
    CCTGATCAGG CCTCTGCTGC TTCTACCGCT CCATCTGCAT CGACAGTAAC CAACGGACGA 
    AGGGAAACCG CTTCAGGCCA AATTCCCACA TTAACAAACC ATGACCAGAA CGGCCTGAAC 
    TACATTGATC TGGATCTAAT GCAGAATGGA GAGCAACATG TTCCTGATTG GAATGCTTTC 
    CAGTCTCGGC CGGTGGAGCT GGGAGCTGTT GAAGGTATAG AAGAGCTCAG TGCCTATGCC 
    AGTATAAACT TCCAGAAATC AGATGAGACC AGAGGGAATC TCACACACAG AGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_687702.4
    CDS1011..4310
    Translation

    Target ORF information:

    RefSeq Version XM_682610.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio insulin receptor substrate 1 (irs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_682610.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCAAGCC CGACTACGGA GCAAGGATGT TTTTCAGACG TGAAAAAGGT GGGTTATTTA 
    AGGAAACCCA AAAGCATGCA CAAAAGGTTT TTTGTACTGA GGGCGGCGAG CGCCTCCGGA 
    CCGTCCCGAC TGGAGTACTA TGAAAACGAG AAGAAGTGGA GACACAAGTC TGGAGCGCCG 
    AAAAGATCCA TTCCGCTGGA GAGCTGCTTT AACATCAACA AAAGAGCGGA TTCAAAAAAC 
    AAACATCTCG TCGCGCTTTA TACCAAGGAT GAGTACTTTG CTATCGCTGC TGACAGCGAG 
    ATCGAGCAAG AGTCATGGTA TGAAGCCCTG GTTGATCTCC ATAACAGAGG TAAGGTCCAC 
    GAAACTGCCC CGGGCCGTGG AGTTGGCGAG GATAATTACG GGGAAGCCAC GCCGGGACCT 
    GCCTTTAAAG AGGTTTGGCA AGTTATATTG AAACCAAAGG GTCTGGGTCA CACAAAGAAC 
    TTGATTGGAG TTTACAGATT ATGCCTCACC AATAAGACTA TCAGCTTTGT GAAACTGAAT 
    TCGGATGCAG CAGCTGTGGT TTTGCAGTTG ATGAACATTA GGAGATGTGG TCACTCTGAA 
    AATTTCTTTT TTATTGAAGT CGGCCGATCT GCTGTGACAG GCCCCGGGGA GTTTTGGATG 
    CAAGTGGATG ACTCTGTTGT GGCTCAGAAC ATGCATGAAA CTATATTAGA AGCCATGAAA 
    GCCATGAGTG AGGAGTTCAG ACCCCGCAGC AAAAGCCAGT CATCTTCTAA CTGCTCAAAC 
    CCCATCTCAG TGCCTGTCAG GCGGCATCAT CACAATAATC CTCCCCCTAG TCAAGTTGGC 
    TTAGGCAGGC GCTCTCGGGC GGAGAGTGTG ACGGCCACTT CTCCTGTTAG GCCTGGCAAA 
    CACAGCCATT CGTTTAGAGT GAGAGCATCT AGTGATGGAG AGGGCACCAT GTCCAGGCCG 
    GCATCTGTGG ACGGGAGTCC AAGCAGCCCG AGCAATGCCC GACCTCAATC GCAACGGCAC 
    AGAGGGGGAT CCAGACTTCA CCCGCCACTC AATCACAGCA GATCAATACC CACGCCCACC 
    TCACGGTGCT CCCCCTCAGC CATCAGCCCC ATCAGCCTGT CCTCCAGCAG CACGAGCGGG 
    CATGGCTCCA CATCTGACTG CCTCTTTCCA CGTCGCTCTA GTGCCTCGAT ATCTGGATCA 
    CCCAGCGATG GCGGATTCAT CTCCTCAGAT GAATATGGAT CCAGCCCCTG TGACTTTCGA 
    AGCTCGTTTC GTAGCGTGAC TCCAGACTCG CTTGGCCACA CACCACCTGC CAGGGAGGAA 
    GAGATAAACA ACTACATCTG CATGGCAAAG CCCGGTTCAC TTCCTGGTGC CGGGAGCCAG 
    TCACGAAGCC AATCTAGAGG AACACCGTCA AGACTAGAGG AGCCTGAGTT GGAAAAATGT 
    TTTCGGAAAA GAACTCATTC TTCTGGTGCT TCGCCGCCAA CTCCATGCCA TCAGAAAACT 
    CCCTCACAAT CGCCTGCTGC ATCTTTAGAG GAATACACTG TGATGATGCC TACATACCCA 
    CGAAGCCGCT CTGCATCATC GTCATCATCA ACATATAGGC ACTCATTCAT GCCAACACAT 
    TCATACCCAG AGGAAGGTAC AGATGCATCA CAAGCAAAAG ATGAAAGGGG GCCAGATCAC 
    AAAGATGACG GCTATATGCC CATGCTGCCA GGCGTTGCGC CTGCAACCCC TACAACCAAA 
    AGCAGTGACT ACATGCCCAT GAGTCCAAAA AGTGTGTCTG CACCACAGCA AATTATCAAC 
    CCCAGGCAAC ACTCGCATGT CGACTCCAAT GGTTACATGA TGATGTCACC AAGTGGCAGC 
    TGCTCTCCAG ATGGCACAAC AAACTATGGC AAGATCTGGA CGAATGGCAT CAACCCTAAG 
    CTCTCCGTAG AGAGTATGGA AGGTAAAGTG TCGTCATGTG GAGATTACAT AAACATGTCT 
    CCAGCTAGTT GCTCAACAAC AAGTACGCCT CCAGACTGCT TTTTCAACCC TGTGGAAGAT 
    CCTCCCAGGC CTATGTATGC TTACTACTCT TTGCCCCGCT CATTCAAACA TGCTAACAGG 
    AAGCAGGATC AGAGCCCGCT TCGAGTGTCA CTGGGCTCGA GTCGATTAGC ATACGCTGAC 
    TCCTCTTCAT CTTCAGCAAG CAGTGACAGC TTGGGTGGTC AAAGCAATTC CCAGCAGTCT 
    GCTGTCAAAC CCAAAAGGTC AGAGGGCAAC GGTAGGCTGA CACGTCCAAC ACGGCTGTCA 
    CTGGACGCCA GCAAAGCAAG TACTCTTCCA CGCACACGTG AGAGCCCCTT CCCTCCTGAG 
    CCTAAAAGTC CTGGGGAGTA TGTCAACATT GAGTTCAACG ACAAGGCATT TTCAGCGAGC 
    TTAGCATCGC TGTTCCCACC TGTTTATTCA GTAAGTGATG CTGAGGCCCC GTCGGATCTT 
    TCATCATCTG AGTACATGAA CATGCAGCTG GGATCCCAGG CCAGACGGCT GTGCTCCGAA 
    TTGCAGACTT CCTCTTCCAG TTCAGATTAT GCCATAATCA CACCGTCAAA CTCTGTATCC 
    TCGCCTCCAC TGCCATGTGA GAGCATATGT GAACGTGACT ACATCAGCAT GCAGTTAGCA 
    TCCTGCACTG GCTTTCCTGA CACACAATTG ATGCTAATGA CCGAGGCCCT GCCAGATGCA 
    GATGCCCCTC CTTGTTCTGT ATCACCCAAT CATGACGTGA CGACATTAAG CGGCGCTCCT 
    GGAAGACCAG CTCTGATTAG CCCTTTATCA GGTCTGAGTG CATTCACACG GGTTAACTCC 
    ACTTCCGGAC GAAACCAGGG CGCTAAAGTA ATACGTGCTG ACCCACAGGG TCGACGGCGG 
    CACAGTTCAG AGACTTTTGC ATCCACAGCG ACCAGAGGGT CAGTCGACAC ATCGAGCACC 
    TGTCAATCAG GGGATGTAAA GCGCCACAGC TCCGCCTCCT TCGAGAATGT GTGGTTGAGA 
    CCTGATCAGG CCTCTGCTGC TTCTACCGCT CCATCTGCAT CGACAGTAAC CAACGGACGA 
    AGGGAAACCG CTTCAGGCCA AATTCCCACA TTAACAAACC ATGACCAGAA CGGCCTGAAC 
    TACATTGATC TGGATCTAAT GCAGAATGGA GAGCAACATG TTCCTGATTG GAATGCTTTC 
    CAGTCTCGGC CGGTGGAGCT GGGAGCTGTT GAAGGTATAG AAGAGCTCAG TGCCTATGCC 
    AGTATAAACT TCCAGAAATC AGATGAGACC AGAGGGAATC TCACACACAG AGAAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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