tfpi2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tfpi2
Entrez Gene ID	560339
Full Name	tissue factor pathway inhibitor 2
Synonyms	si:ch211-262k23.2
General protein information	Preferred Names tissue factor pathway inhibitor 2 Names tissue factor pathway inhibitor 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040096.2	NP_001035185.1	tissue factor pathway inhibitor 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TFPI2	XP_001168275.1
Canis lupus familiaris (dog)	TFPI2	XP_532462.3
Mus musculus (house mouse)	Tfpi2	NP_033390.1
Danio rerio (zebrafish)	tfpi2	NP_001035185.1
Homo sapiens (human)	TFPI2	NP_006519.1
	TFPI2	XP_001099023.1
Bos taurus (cattle)	TFPI2	NP_877589.1
Rattus norvegicus (Norway rat)	Tfpi2	NP_775164.1
Gallus gallus (chicken)	TFPI2	XP_418662.2
Xenopus tropicalis (tropical clawed frog)	tfpi2	NP_001015766.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Gistelinck C, Gioia R, Gagliardi A, Tonelli F, Marchese L, Bianchi L, Landi C, Bini L, Huysseune A, Witten PE, Staes A, Gevaert K, De Rocker N, Menten B, Malfait F, Leikin S, Carra S, Tenni R, Rossi A, De Paepe A, Coucke P, Willaert A, Forlino A
Scientific reports621540(2016 Feb)

Tissue factor pathway inhibitor-2 is critical in zebrafish cardiogenesis.
Zhang Y, Wang H, Wen D, Zhang J, Zheng F, Jiang N, Ma D
Biochemical and biophysical research communications456(3)827-33(2015 Jan)

Tissue factor pathway inhibitor-2: a novel gene involved in zebrafish central nervous system development.
Zhang Y, Wang L, Zhou W, Wang H, Zhang J, Deng S, Li W, Li H, Mao Z, Ma D
Developmental biology381(1)38-49(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taken together, these results demonstrated a critical role of zTfpi-2 during embryonic cardiac development, as well as an important regulator of hematopoiesis.
Title: Tissue factor pathway inhibitor-2 is critical in zebrafish cardiogenesis.

zTfpi2 is involved in zebrafish central nervous system development
Title: Tissue factor pathway inhibitor-2: a novel gene involved in zebrafish central nervous system development.

The following tfpi2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tfpi2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00501	NM_001040096.2 Latest version!	Danio rerio tissue factor pathway inhibitor 2 (tfpi2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tfpi2 ( NM_001040096.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001035185.1 Danio rerio tissue factor pathway inhibitor 2 (tfpi2), mRNA.

Price & Availability↑

CloneID	ODa00501
Clone ID Related Accession (Same CDS sequence)	NM_001040096.2
Accession Version	NM_001040096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	tissue factor pathway inhibitor 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125893.1. On Oct 29, 2006 this sequence version replaced NM_001040096.1. ##Evidence-Data-START## Transcript exon combination :: BC125893.1, CK141863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTGTG ATTTACTCGG AGCTCTGTTG CTTTTCATTT CTGTGCTAGA AAGCGCCGTC 
GGACTGACGT TACAACCTAA AGAAGTTTGT CTGTTGCAAA TTGAAGAAGG GACGTGTAAT 
GACGACATTC AGCGGTTTTA CTACAATACA ATCAGCCAGC AGTGTGAAGA GTTCAGCTAC 
AGCGGCTGTG GAGGAAACCA AAACAACTTC AGGTCTTTCG TGGAATGTCA GAAAACATGC 
TTCAGGATAC CAAAAATCCC CCAGATCTGT CGTTTTCAAA AGAAAGAGGG GCCCTGCCGT 
GGCCTCTTCA GCCGTTATTT CTTCAATATG ACCTCCATGC AGTGTGAACC ATTCACTTAT 
GGTGGCTGCC AAGGGAATGA GAACAATTTT CGAAACCCAG AGGAATGCAT AGAATATTGC 
AAACCTCCGA AAACGATCCC TGTGATTTGC CTGGATAATT TGGATAAAGG AAGATGCTCA 
GCATCCATAC CAAGGTATTA TTACAACTCT GCCACAAAGA CATGCGAGGA GTTCATGTAC 
ACCGGATGCG GAGGAAGCAA CAATAACTTC ATCTCCAAGC AAAGCTGCGT CGACGTTTGT 
GGGAGCAAAC GTTGGAGTCC CACTAAAAAA TCGGTGAGAG TTTCTAAGCA GTACCTGAGA 
CGAGTGAAAC CTCAGCCATC GAGGGAGAAG ACGAACAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035185.1
CDS	59..760
Translation	MACDLLGALLLFISVLESAVGLTLQPKEVCLLQIEEGTCNDDIQRFYYNTISQQCEEFSYSGCGGNQNNFRSFVECQKTCFRIPKIPQICRFQKKEGPCRGLFSRYFFNMTSMQCEPFTYGGCQGNENNFRNPEECIEYCKPPKTIPVICLDNLDKGRCSASIPRYYYNSATKTCEEFMYTGCGGSNNNFISKQSCVDVCGSKRWSPTKKSVRVSKQYLRRVKPQPSREKTNK

Target ORF information:

RefSeq Version	NM_001040096.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tissue factor pathway inhibitor 2 (tfpi2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040096.2

ORF Insert Sequence:

ATGGCGTGTG ATTTACTCGG AGCTCTGTTG CTTTTCATTT CTGTGCTAGA AAGCGCCGTC 
GGACTGACGT TACAACCTAA AGAAGTTTGT CTGTTGCAAA TTGAAGAAGG GACGTGTAAT 
GACGACATTC AGCGGTTTTA CTACAATACA ATCAGCCAGC AGTGTGAAGA GTTCAGCTAC 
AGCGGCTGTG GAGGAAACCA AAACAACTTC AGGTCTTTCG TGGAATGTCA GAAAACATGC 
TTCAGGATAC CAAAAATCCC CCAGATCTGT CGTTTTCAAA AGAAAGAGGG GCCCTGCCGT 
GGCCTCTTCA GCCGTTATTT CTTCAATATG ACCTCCATGC AGTGTGAACC ATTCACTTAT 
GGTGGCTGCC AAGGGAATGA GAACAATTTT CGAAACCCAG AGGAATGCAT AGAATATTGC 
AAACCTCCGA AAACGATCCC TGTGATTTGC CTGGATAATT TGGATAAAGG AAGATGCTCA 
GCATCCATAC CAAGGTATTA TTACAACTCT GCCACAAAGA CATGCGAGGA GTTCATGTAC 
ACCGGATGCG GAGGAAGCAA CAATAACTTC ATCTCCAAGC AAAGCTGCGT CGACGTTTGT 
GGGAGCAAAC GTTGGAGTCC CACTAAAAAA TCGGTGAGAG TTTCTAAGCA GTACCTGAGA 
CGAGTGAAAC CTCAGCCATC GAGGGAGAAG ACGAACAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Scientific reports621540(2016 Feb)
Gistelinck C,Gioia R,Gagliardi A,Tonelli F,Marchese L,Bianchi L,Landi C,Bini L,Huysseune A,Witten PE,Staes A,Gevaert K,De Rocker N,Menten B,Malfait F,Leikin S,Carra S,Tenni R,Rossi A,De Paepe A,Coucke P,Willaert A,Forlino A

Tissue factor pathway inhibitor-2 is critical in zebrafish cardiogenesis.
Biochemical and biophysical research communications456(3)827-33(2015 Jan)
Zhang Y,Wang H,Wen D,Zhang J,Zheng F,Jiang N,Ma D

Tissue factor pathway inhibitor-2: a novel gene involved in zebrafish central nervous system development.
Developmental biology381(1)38-49(2013 Sep)
Zhang Y,Wang L,Zhou W,Wang H,Zhang J,Deng S,Li W,Li H,Mao Z,Ma D

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tfpi2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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