si:ch73-103b11.2 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005162905.4	XP_005162962.1	myosin phosphatase Rho-interacting protein isoform X6
XM_005162902.3	XP_005162959.1	centrosome-associated protein CEP250 isoform X3
XM_005162900.3	XP_005162957.1	centrosome-associated protein CEP250 isoform X1
XM_005162903.3	XP_005162960.1	centrosome-associated protein CEP250 isoform X4
XM_005162906.3	XP_005162963.1	myosin phosphatase Rho-interacting protein isoform X6
XM_021470411.1	XP_021326086.1	early endosome antigen 1 isoform X5
XM_005162906.4	XP_005162963.1	myosin phosphatase Rho-interacting protein isoform X7
XM_005162901.3	XP_005162958.1	centrosome-associated protein CEP250 isoform X2
XM_009297658.2	XP_009295933.1	centrosome-associated protein CEP250 isoform X3
XM_005162905.3	XP_005162962.1	myosin phosphatase Rho-interacting protein isoform X5
XM_005162901.4	XP_005162958.1	centrosome-associated protein CEP250 isoform X2
XM_009297658.3	XP_009295933.1	centrosome-associated protein CEP250 isoform X3
XM_005162900.4	XP_005162957.1	centrosome-associated protein CEP250 isoform X1
XM_005162902.4	XP_005162959.1	centrosome-associated protein CEP250 isoform X3
XM_005162903.4	XP_005162960.1	centrosome-associated protein CEP250 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa28906	XM_005162905.4 Latest version!	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa28907	XM_005162906.4 Latest version!	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa141286	XM_021470411.1 Latest version!	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa28901	XM_005162900.4 Latest version!	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa28902	XM_005162901.4 Latest version!	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa28903	XM_005162902.4 Latest version!	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa28903	XM_009297658.3 Latest version!	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa28904	XM_005162903.4 Latest version!	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa28907	XM_005162906.3	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa28906	XM_005162905.3	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa28902	XM_005162901.3	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa28903	XM_009297658.2	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa28901	XM_005162900.3	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa28903	XM_005162902.3	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa28904	XM_005162903.3	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

si:ch73-103b11.2 ( XM_005162905.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162962.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa28906
Clone ID Related Accession (Same CDS sequence)	XM_005162905.4 , XM_005162905.3
Accession Version	XM_005162905.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162905.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGGC CACATGCGAG CGTGGATTTG CCGCCATGGA GGAGACGCAT 
CAGAAAGTGA TTGAAGACAT CCAGAGGCAG CATCAGCGGG AGATCCAGAA ACTTCTAGAG 
GAGAAGGAAA GACTGCTGGA GGAGGAAACA AACGCCACTA TTGCTGCAAT TGAGGCCATG 
AAAAATGCTC ATCGTGAGGA ACTGGAGAAA ACGCAGCGGT CGCAGATGAG CGGTGTGAGC 
GCAGATATTC AGGAGCTGCA CAGACAGTAT GAAGAGGAGT TGCAGTCTAT CCATCGGGAG 
CTGGAGGTTT TGTCTGAGCA GTATTCTCAG AAGTGTCTGG AAAACGCACA TTTGGCTCAA 
GCGCTGGAAG CTGAGAGACA GGCGCTGGGA CAGTGTCAGC GAGAGAACCA GAAACTGCAT 
ATACACAACC AGGAACTAAA CCATCGTCTG AATGAGGAGA TCACCAGAAT GCACTCCTGC 
ATCAGCGGAG AAAAAAACAC CTCTTCTCTG ACGCAAGGCA AAGACATCTA TGAGCTGGAG 
GTTTTGCTAC GAGTGAAGGA GTCCGAGATC CAGTACCTCA AACAAGAAAT AAACTCTCTG 
AAGGACGAGC TGCAGTCGGC ACTTCGAGAT AAGAAGTATG CTTCAGATAA ATACAAGGAC 
ATTTACACCG AGCTGAGCAT TGTGAAAGCT AAAGCTGACT GTGACATTAA CAAGCTGAGG 
GAGAAACTTT TGGCTGCTAC AGAAGCTCTT GGGGAACTAG ACGCTGAGGG AAGCGGTGTT 
ACGGCTGGAT ACGACATTAT GAAGTCAAAG AGTAACCCAG ATTTCCTGAA GAAGGAAAAA 
TCCAGACAGA TGCGTGGAGT CAGGTCAAAG AGTTTAAAGG AGGGCTTGAC CGTACAGGAG 
AGGATGAAGC TCTTCGAAGC GAAAGACTCC AGGAAGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162962.1
CDS	629..4390
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162905.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162905.4

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGGC CACATGCGAG CGTGGATTTG CCGCCATGGA GGAGACGCAT 
CAGAAAGTGA TTGAAGACAT CCAGAGGCAG CATCAGCGGG AGATCCAGAA ACTTCTAGAG 
GAGAAGGAAA GACTGCTGGA GGAGGAAACA AACGCCACTA TTGCTGCAAT TGAGGCCATG 
AAAAATGCTC ATCGTGAGGA ACTGGAGAAA ACGCAGCGGT CGCAGATGAG CGGTGTGAGC 
GCAGATATTC AGGAGCTGCA CAGACAGTAT GAAGAGGAGT TGCAGTCTAT CCATCGGGAG 
CTGGAGGTTT TGTCTGAGCA GTATTCTCAG AAGTGTCTGG AAAACGCACA TTTGGCTCAA 
GCGCTGGAAG CTGAGAGACA GGCGCTGGGA CAGTGTCAGC GAGAGAACCA GAAACTGCAT 
ATACACAACC AGGAACTAAA CCATCGTCTG AATGAGGAGA TCACCAGAAT GCACTCCTGC 
ATCAGCGGAG AAAAAAACAC CTCTTCTCTG ACGCAAGGCA AAGACATCTA TGAGCTGGAG 
GTTTTGCTAC GAGTGAAGGA GTCCGAGATC CAGTACCTCA AACAAGAAAT AAACTCTCTG 
AAGGACGAGC TGCAGTCGGC ACTTCGAGAT AAGAAGTATG CTTCAGATAA ATACAAGGAC 
ATTTACACCG AGCTGAGCAT TGTGAAAGCT AAAGCTGACT GTGACATTAA CAAGCTGAGG 
GAGAAACTTT TGGCTGCTAC AGAAGCTCTT GGGGAACTAG ACGCTGAGGG AAGCGGTGTT 
ACGGCTGGAT ACGACATTAT GAAGTCAAAG AGTAACCCAG ATTTCCTGAA GAAGGAAAAA 
TCCAGACAGA TGCGTGGAGT CAGGTCAAAG AGTTTAAAGG AGGGCTTGAC CGTACAGGAG 
AGGATGAAGC TCTTCGAAGC GAAAGACTCC AGGAAGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162905.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162962.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa28906
Clone ID Related Accession (Same CDS sequence)	XM_005162905.4 , XM_005162905.3
Accession Version	XM_005162905.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162905.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGGC CACATGCGAG CGTGGATTTG CCGCCATGGA GGAGACGCAT 
CAGAAAGTGA TTGAAGACAT CCAGAGGCAG CATCAGCGGG AGATCCAGAA ACTTCTAGAG 
GAGAAGGAAA GACTGCTGGA GGAGGAAACA AACGCCACTA TTGCTGCAAT TGAGGCCATG 
AAAAATGCTC ATCGTGAGGA ACTGGAGAAA ACGCAGCGGT CGCAGATGAG CGGTGTGAGC 
GCAGATATTC AGGAGCTGCA CAGACAGTAT GAAGAGGAGT TGCAGTCTAT CCATCGGGAG 
CTGGAGGTTT TGTCTGAGCA GTATTCTCAG AAGTGTCTGG AAAACGCACA TTTGGCTCAA 
GCGCTGGAAG CTGAGAGACA GGCGCTGGGA CAGTGTCAGC GAGAGAACCA GAAACTGCAT 
ATACACAACC AGGAACTAAA CCATCGTCTG AATGAGGAGA TCACCAGAAT GCACTCCTGC 
ATCAGCGGAG AAAAAAACAC CTCTTCTCTG ACGCAAGGCA AAGACATCTA TGAGCTGGAG 
GTTTTGCTAC GAGTGAAGGA GTCCGAGATC CAGTACCTCA AACAAGAAAT AAACTCTCTG 
AAGGACGAGC TGCAGTCGGC ACTTCGAGAT AAGAAGTATG CTTCAGATAA ATACAAGGAC 
ATTTACACCG AGCTGAGCAT TGTGAAAGCT AAAGCTGACT GTGACATTAA CAAGCTGAGG 
GAGAAACTTT TGGCTGCTAC AGAAGCTCTT GGGGAACTAG ACGCTGAGGG AAGCGGTGTT 
ACGGCTGGAT ACGACATTAT GAAGTCAAAG AGTAACCCAG ATTTCCTGAA GAAGGAAAAA 
TCCAGACAGA TGCGTGGAGT CAGGTCAAAG AGTTTAAAGG AGGGCTTGAC CGTACAGGAG 
AGGATGAAGC TCTTCGAAGC GAAAGACTCC AGGAAGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162962.1
CDS	626..4387
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162905.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162905.3

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGGC CACATGCGAG CGTGGATTTG CCGCCATGGA GGAGACGCAT 
CAGAAAGTGA TTGAAGACAT CCAGAGGCAG CATCAGCGGG AGATCCAGAA ACTTCTAGAG 
GAGAAGGAAA GACTGCTGGA GGAGGAAACA AACGCCACTA TTGCTGCAAT TGAGGCCATG 
AAAAATGCTC ATCGTGAGGA ACTGGAGAAA ACGCAGCGGT CGCAGATGAG CGGTGTGAGC 
GCAGATATTC AGGAGCTGCA CAGACAGTAT GAAGAGGAGT TGCAGTCTAT CCATCGGGAG 
CTGGAGGTTT TGTCTGAGCA GTATTCTCAG AAGTGTCTGG AAAACGCACA TTTGGCTCAA 
GCGCTGGAAG CTGAGAGACA GGCGCTGGGA CAGTGTCAGC GAGAGAACCA GAAACTGCAT 
ATACACAACC AGGAACTAAA CCATCGTCTG AATGAGGAGA TCACCAGAAT GCACTCCTGC 
ATCAGCGGAG AAAAAAACAC CTCTTCTCTG ACGCAAGGCA AAGACATCTA TGAGCTGGAG 
GTTTTGCTAC GAGTGAAGGA GTCCGAGATC CAGTACCTCA AACAAGAAAT AAACTCTCTG 
AAGGACGAGC TGCAGTCGGC ACTTCGAGAT AAGAAGTATG CTTCAGATAA ATACAAGGAC 
ATTTACACCG AGCTGAGCAT TGTGAAAGCT AAAGCTGACT GTGACATTAA CAAGCTGAGG 
GAGAAACTTT TGGCTGCTAC AGAAGCTCTT GGGGAACTAG ACGCTGAGGG AAGCGGTGTT 
ACGGCTGGAT ACGACATTAT GAAGTCAAAG AGTAACCCAG ATTTCCTGAA GAAGGAAAAA 
TCCAGACAGA TGCGTGGAGT CAGGTCAAAG AGTTTAAAGG AGGGCTTGAC CGTACAGGAG 
AGGATGAAGC TCTTCGAAGC GAAAGACTCC AGGAAGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162906.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162963.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X7, mRNA.

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CloneID	ODa28907
Clone ID Related Accession (Same CDS sequence)	XM_005162906.3 , XM_005162906.4
Accession Version	XM_005162906.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162906.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GTTCCCTTCC GGATGAACGA 
ACCAGATCAA ATGTGGAGCA GAGCTTCATC CAACCGCCAT CACAGCTCTC CAACAGCTCG 
ATAGTTGAGC CAAAAAGGCC ACATGAGGAC ATCACCGACT CCGCACCTCC TCCAGATCAG 
CGTAAAAGCA GAGGCCACAA GCGTGAGGGC CGGTCCAAAA CCTTCGACTG GTCAGAATTT 
CGACCAGGAC AAGAAAACGA AAGTGGACAA CCCTCACTAA AGAGAGCAGA TACTTCAGTC 
ATTATCTCAT CTTCTCCATC ATCCATCGCT TCCTCTCCCA TCTCTAGCTC TTCTTCTCAT 
CAGACGTCTT CCTCCATCAC GATCATCAGC CATACTGAAA AGTTGCCACA GCATAATGAA 
GAAGAAGAAC AAGTGGTGCA GGAGTATGTA CCGCACCATC AGACTGTACC TACAACAATG 
ACCGGTCCAG AGACCCCGTC AAACCCTCAT AATGTGCCAC AGGAACCGGC AGGAATAGAT 
TTGAACCAGT CCAGAGAAGT GCAGGAGGTC GATGGTCATG GAGTGATCCG CAAAAACTCT 
GATGTCCAAG TGGAAATTGA ACAGAGGTGG CATCAGGTGG AAACGACGCC CCTCAGGGAG 
GAAAAGCAGG TACCGATCGC TGGTGGCTCA CCTAACGTAT CAAATGTGTC TGTTGGAGAA 
AGAGCCTCTC CGCAAGAGAA GACCAGCAAA TTGGAAAAAG AGTTGGGTCA GGCACAAAAG 
GAGTTGGCCA GACTCCAGGA GCAGAACAGC ATCTTACAAG ACCAACTTCA GGATGCACGC 
GGGAGAGAGC AGAATGCACG GGAAGGATAT GTACTTCAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GAGTTTAAAG 
GAGGGCTTGA CCGTACAGGA GAGGATGAAG CTCTTCGAAG CGAAAGACTC CAGGAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162963.1
CDS	626..4348
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162906.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162906.3

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GTTCCCTTCC GGATGAACGA 
ACCAGATCAA ATGTGGAGCA GAGCTTCATC CAACCGCCAT CACAGCTCTC CAACAGCTCG 
ATAGTTGAGC CAAAAAGGCC ACATGAGGAC ATCACCGACT CCGCACCTCC TCCAGATCAG 
CGTAAAAGCA GAGGCCACAA GCGTGAGGGC CGGTCCAAAA CCTTCGACTG GTCAGAATTT 
CGACCAGGAC AAGAAAACGA AAGTGGACAA CCCTCACTAA AGAGAGCAGA TACTTCAGTC 
ATTATCTCAT CTTCTCCATC ATCCATCGCT TCCTCTCCCA TCTCTAGCTC TTCTTCTCAT 
CAGACGTCTT CCTCCATCAC GATCATCAGC CATACTGAAA AGTTGCCACA GCATAATGAA 
GAAGAAGAAC AAGTGGTGCA GGAGTATGTA CCGCACCATC AGACTGTACC TACAACAATG 
ACCGGTCCAG AGACCCCGTC AAACCCTCAT AATGTGCCAC AGGAACCGGC AGGAATAGAT 
TTGAACCAGT CCAGAGAAGT GCAGGAGGTC GATGGTCATG GAGTGATCCG CAAAAACTCT 
GATGTCCAAG TGGAAATTGA ACAGAGGTGG CATCAGGTGG AAACGACGCC CCTCAGGGAG 
GAAAAGCAGG TACCGATCGC TGGTGGCTCA CCTAACGTAT CAAATGTGTC TGTTGGAGAA 
AGAGCCTCTC CGCAAGAGAA GACCAGCAAA TTGGAAAAAG AGTTGGGTCA GGCACAAAAG 
GAGTTGGCCA GACTCCAGGA GCAGAACAGC ATCTTACAAG ACCAACTTCA GGATGCACGC 
GGGAGAGAGC AGAATGCACG GGAAGGATAT GTACTTCAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GAGTTTAAAG 
GAGGGCTTGA CCGTACAGGA GAGGATGAAG CTCTTCGAAG CGAAAGACTC CAGGAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162906.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162963.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X8, mRNA.

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CloneID	ODa28907
Clone ID Related Accession (Same CDS sequence)	XM_005162906.3 , XM_005162906.4
Accession Version	XM_005162906.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin phosphatase Rho-interacting protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162906.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GTTCCCTTCC GGATGAACGA 
ACCAGATCAA ATGTGGAGCA GAGCTTCATC CAACCGCCAT CACAGCTCTC CAACAGCTCG 
ATAGTTGAGC CAAAAAGGCC ACATGAGGAC ATCACCGACT CCGCACCTCC TCCAGATCAG 
CGTAAAAGCA GAGGCCACAA GCGTGAGGGC CGGTCCAAAA CCTTCGACTG GTCAGAATTT 
CGACCAGGAC AAGAAAACGA AAGTGGACAA CCCTCACTAA AGAGAGCAGA TACTTCAGTC 
ATTATCTCAT CTTCTCCATC ATCCATCGCT TCCTCTCCCA TCTCTAGCTC TTCTTCTCAT 
CAGACGTCTT CCTCCATCAC GATCATCAGC CATACTGAAA AGTTGCCACA GCATAATGAA 
GAAGAAGAAC AAGTGGTGCA GGAGTATGTA CCGCACCATC AGACTGTACC TACAACAATG 
ACCGGTCCAG AGACCCCGTC AAACCCTCAT AATGTGCCAC AGGAACCGGC AGGAATAGAT 
TTGAACCAGT CCAGAGAAGT GCAGGAGGTC GATGGTCATG GAGTGATCCG CAAAAACTCT 
GATGTCCAAG TGGAAATTGA ACAGAGGTGG CATCAGGTGG AAACGACGCC CCTCAGGGAG 
GAAAAGCAGG TACCGATCGC TGGTGGCTCA CCTAACGTAT CAAATGTGTC TGTTGGAGAA 
AGAGCCTCTC CGCAAGAGAA GACCAGCAAA TTGGAAAAAG AGTTGGGTCA GGCACAAAAG 
GAGTTGGCCA GACTCCAGGA GCAGAACAGC ATCTTACAAG ACCAACTTCA GGATGCACGC 
GGGAGAGAGC AGAATGCACG GGAAGGATAT GTACTTCAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GAGTTTAAAG 
GAGGGCTTGA CCGTACAGGA GAGGATGAAG CTCTTCGAAG CGAAAGACTC CAGGAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162963.1
CDS	629..4351
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162906.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162906.4

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GTTCCCTTCC GGATGAACGA 
ACCAGATCAA ATGTGGAGCA GAGCTTCATC CAACCGCCAT CACAGCTCTC CAACAGCTCG 
ATAGTTGAGC CAAAAAGGCC ACATGAGGAC ATCACCGACT CCGCACCTCC TCCAGATCAG 
CGTAAAAGCA GAGGCCACAA GCGTGAGGGC CGGTCCAAAA CCTTCGACTG GTCAGAATTT 
CGACCAGGAC AAGAAAACGA AAGTGGACAA CCCTCACTAA AGAGAGCAGA TACTTCAGTC 
ATTATCTCAT CTTCTCCATC ATCCATCGCT TCCTCTCCCA TCTCTAGCTC TTCTTCTCAT 
CAGACGTCTT CCTCCATCAC GATCATCAGC CATACTGAAA AGTTGCCACA GCATAATGAA 
GAAGAAGAAC AAGTGGTGCA GGAGTATGTA CCGCACCATC AGACTGTACC TACAACAATG 
ACCGGTCCAG AGACCCCGTC AAACCCTCAT AATGTGCCAC AGGAACCGGC AGGAATAGAT 
TTGAACCAGT CCAGAGAAGT GCAGGAGGTC GATGGTCATG GAGTGATCCG CAAAAACTCT 
GATGTCCAAG TGGAAATTGA ACAGAGGTGG CATCAGGTGG AAACGACGCC CCTCAGGGAG 
GAAAAGCAGG TACCGATCGC TGGTGGCTCA CCTAACGTAT CAAATGTGTC TGTTGGAGAA 
AGAGCCTCTC CGCAAGAGAA GACCAGCAAA TTGGAAAAAG AGTTGGGTCA GGCACAAAAG 
GAGTTGGCCA GACTCCAGGA GCAGAACAGC ATCTTACAAG ACCAACTTCA GGATGCACGC 
GGGAGAGAGC AGAATGCACG GGAAGGATAT GTACTTCAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GAGTTTAAAG 
GAGGGCTTGA CCGTACAGGA GAGGATGAAG CTCTTCGAAG CGAAAGACTC CAGGAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_021470411.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326086.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa141286
Clone ID Related Accession (Same CDS sequence)	XM_021470411.1
Accession Version	XM_021470411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	early endosome antigen 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATAC ACACAGAAAG GCGGCATGTG AGTCAGAAGC GTGTGAGGAA CAGGTCACAG 
ATGAAACTCA ACAAGACTAA GCCTGACCTG CTGAACTTTA AGAAAGGATG GATGACCAAA 
CTATACGAAG ATGGATTGTG GAAGAAACAC TGGTTTGTTC TGACCGATCA GAGTCTGAGG 
TTCTACCGTG ACTCCATTGC TGAGGAGGCT TCTGATCTGG ATGGAGAGAT CGATCTGTCC 
TTGTGTTATG ATGTGACCGA ATTCCCAGTT CAGAGGAACT ACGGCTTCCA AATCCTCAGC 
AAAGAAGGGG CGTTCACACT GTCTGCTATG ACTTCTGGGA TACGTCGTAA CTGGATTCAA 
GCCATTCTGA AAAACATGCG GCCCACTGTC GCTCCTGATG TCACACGGAA AAACGTCTCT 
CTGAAACTTT CAGTTCTTAT GCCCAGTTCC CTTCCGGATG AACGAACCAG ATCAAATGTG 
GAGCAGAGCT TCATCCAACC GCCATCACAG CTCTCCAACA GCTCGATAGT TGAGCCAAAA 
AGGCCACATG AGGACATCAC CGACTCCGCA CCTCCTCCAG ATCAGCGTAA AAGCAGAGGC 
CACAAGCGTG AGGGCCGGTC CAAAACCTTC GACTGGTCAG AATTTCGACC AGGACAAGAA 
AACGAAAGTG GACAACCCTC ACTAAAGAGA GCAGATACTT CAGTCATTAT CTCATCTTCT 
CCATCATCCA TCGCTTCCTC TCCCATCTCT AGCTCTTCTT CTCATCAGAC GTCTTCCTCC 
ATCACGATCA TCAGCCATAC TGAAAAGTTG CCACAGCATA ATGAAGAAGA AGAACAAGTG 
GTGCAGGAGT ATGTACCGCA CCATCAGACT GTACCTACAA CAATGACCGG TCCAGAGACC 
CCGTCAAACC CTCATAATGT GCCACAGGAA CCGGCAGGAA TAGATTTGAA CCAGTCCAGA 
GAAGTGCAGG AGGTCGATGG TCATGGAGTG ATCCGCAAAA ACTCTGATGT CCAAGTGGAA 
ATTGAACAGA GGTGGCATCA GGTGGAAACG ACGCCCCTCA GGGAGGAAAA GCAGGTACCG 
ATCGCTGGTG GCTCACCTAA CGTATCAAAT GTGTCTGTTG GAGAAAGAGC CTCTCCGCAA 
GAGAAGACCA GCAAATTGGA AAAAGAGTTG GGTCAGGCAC AAAAGGAGTT GGCCAGACTC 
CAGGAGCAGA ACAGCATCTT ACAAGACCAA CTTCAGGATG CACGCGGGAG AGAGCAGAAT 
GCACGGGAAG GATATGTACT TCAGAATGAC TCGACTATAG CAAAGCCTTC ATCTGATGCT 
CCCGTGTCTA CCACGGTTCA TAAAGCACCA TGGCAGCGCC TAAGCAAACT AAACCAGGAT 
CTAAAGTCTG AGCTGGAGTC TCAGCGGCGG AAGCAAGATA AGGCCAAACA TCAAGTTAGC 
AGCTTGCGTC GTAGCTACAG TGAAGCAAAA GATATAATAG GGCACCATGA GGCTGAAATC 
GAGGCCTTGG AAGAAAAGCT AACGGCTGCC ATGGCAGAGA TTGTAGTGAG TGAGCAGGCT 
GTAGCACGAA TGCGCAGTGA TCTGAAAATT GAACGCGTTC GTTGTCAAGA GCGTGAGGAA 
GAATGGACAC GAAATGAGAC AACATTACGG TCCCAGCTAC AAGATAGTGA AGAACGTCTT 
CGGCAAGTGG AGGCCAGTTT GCTTGAGAAG AATCAAGAGC TGAGGCAGCT GGAGCAGCAG 
CAAGCTTTAC AAAGGGACCA GCACAGAGAG ATGCAGAGGT TACAGGAGAG ACTCGCTGAG 
GCTACAGGGC GTCTGATTTC CATGGAGGAG TCTCAGACTA TGAGGGAGGA AAGGGAGAGA 
AAAGAGCACC GGAGTCTGGA GGAGAAACAT GAGCGGGAAC GACAAGGGCT GACTCGAAGG 
TTGGCAGAAT CTGAAGAAAG GAGACAAGAA ATAGAAGAGC AATTGCAGGA GGCTCAAGAA 
CAGGTGGAGG CACTGCTGAG GGGCAGCAGT GGGATGGAAA CTGTGGGATT GAGGGAGCAA 
GTGCATCGTC TTCAGCAGGA GTTGGAGACA CAGACTGATA TGGTGGAAAT GCTGCGAGAA 
AGTGTGAGAA GGCTGGAGGA GGAACGTGAC CAGCTTACTT GCCGCTGTCA GGAACTCCTC 
AATCAGATTG CAGAGGCTGA CAGAGAGGTT GGAAAGCAAC AGGCATGTTT AACAACGGTG 
GAAACAGACT TTCACTCCCT TGAGACCTCA TATGAGCATG TATCTGAGGA ATTTGCTAGG 
ATAAGTCATG TGCTACAAGA GAAAGAAGAG GAAGTGAGGC AGACAAAGGA AATGTATGAG 
CATCTTATCA GACAAAAGGA GCAGGACTTG AGTGAAGCCC TCATCAAGAT GGCTGCCTTA 
GGCAGTAGTT TAGAAGAAAC AGAACTTTGC TTGCAGCAAA AAGAAGAACT TCTTTCTAAA 
ATGGAGCGTG ACTATCCAGG ACTGGTAGAG TCCCGAAAAG CAGAACAGGA GCTGCAGGCA 
AAACTGGTAG TTGCAGAGGA TCGAATCGCT GAGCTGGAGG AACACCTCAA TGCCCTGCGA 
CTGGGATATG CAGATCTCAG AATGCGACGT TGTCGCTCTC AGGAAGACAT GCTTGAGAGT 
TTAAAGGAGA TTGAACATGA AGTTTCCCTA TCCTCAAGTA CATCCCAGCT TCTTGCTAGA 
AGCAGTTCAG AGACTGAAAT GTCATTAGTA AAGCGACAGA GGATTCGCTT TTCCACTATC 
CAGTGCCAAA CCTACCACCG GTCCCAGGGA GCAGACAAGT TTCAGACAGA AAACACATCT 
TTGGATCTTA CACAAGATCT GAGTCTAGAC CACATGCATG ACCTGAGCCT GACTCAAGAT 
ATTAGTGTAG TCAGTGACAA TTCATTTCAG CAATGCAGAG ATCCTGAAAA GTTCATCTCC 
ATCATACATG CTCTTGAAAC CAAGCTGTTA GCCACTGAGG AGAAACTCCG GCACCTTACG 
GATAAGATAC GGGACCAGCC CAATCTTTCA GCGGTGCAGG AACATCTCAG TACTAGAGCT 
ACCATGGAGG ACTGGACAAC TAAAACCTGT GATGACTTGC CAATGGATGA ACCCAGCCAG 
AAGCACCTAA ACAAGACTGT CAGTGAACAT GACAGTGGTG AATATGAGAA GGCATTGGCA 
CTGGTAGAGT CTTGCACTGA AAGAGTCAGA GAGATTCTCA GTAATCAGAA TGAGACCAGT 
TCTGCTGAAT CTCTGATTTG TACACTTGCA GAAGTAGAGA AAAGGCTGGT TTGTGCAACT 
GCATTCCTAA GGAAAGGCCA CACATTATAT GAGTCGTGCC ACGTATTTGA TGCACCAGAT 
GTGCAGTCAA TGAAGGGAAG TATTAAGCTG TTTGCCAGAA CATTGTGTTT TGAGGCTGAA 
GTTCTGGAGA AGATGGGGTT CTCTTTGCAG GACTTGAACT CTGATATTAT GGCAGCCCTG 
AACTCCATTC ACAAGGAGGC TGAAAACATC AAGAAGAGCT GTGATGGCTG TCTCTCAGTT 
GTCTATGCTG ATTTGCTTAC AAGGAAACTT CTGCTAGAAA CCATGTTCAT GTCAGAGATA 
GACAAACTTG AGACAGGAAA TCTAGCGAAT AGTGGTTTTT CACAGGATGC CATTAAGAGT 
GCATGCATTA GTGCTGAGCT TGCATATTCA CTTCAGAACC TAACAACGAA TTTCCAGGAA 
AGATTTGATG AGCTTCAAAA GGACTTGATT CAGGCTAACA AGACCTTGAA ACAAAGGGAC 
TTTGCTCTGA AAGATGCTGT TAGTGCATCA AAGCATTCAT GCATTGAAAG CATCCCCCAA 
AGTACGACCG ATCATCTTGT TGGTGATGAT GCCCCTCCTG AACTTGTTCC CTATATGGAG 
CAGATTGAGA TGGAGGAAGC TCAGAGTTTA GCTGCGGAGA TTGTTCGTAG ACATTTAACA 
GGTGGCATGC TATCCCACAG TGCTGAATCA GAATCACATC TCCAAATGGG CTGGGAAAAT 
CTTATTGCTG AGCTGAAGAA GCAAGCCAAA GCTCTTCGCA ATATTTCTCA GGAAATTGAG 
AAGATCTGCG AGACGGGAGA AATGAATTCC CTCTCCAGAC TTGCTGAGGC AATCCAATTA 
AGCTCATACC GCAGCGACTC CAGCTCTGTT TGTATGCGAG AGGCTTTAAT GCAAGTTCAG 
GTCGCCTATG TTTCTTGCAG GTTGAGAGCG GGCCACACAA GGGAACTTTC CCTGTGCCAG 
GAGACCAGCC GCAACATGTC AGCGCTCGTC CAGGAACATG CAGAGAGTGT TGCTGCCATA 
CAAAAGCACT ACCAGAGCTG CTTAGAGAAA GAGCATCTTA GCTTCACAGG CACCATCAGT 
TCCTTGCAGG AGGAGAATGA CATGCTACGA GGGGAGCTAT CCTATAAGCT CAGAGAGCTG 
CAAGAGCAAC GGCAGAACCT GTCCCAGCTG GAAGAAGCAT TTCATAGGGA GAAAGAAGAG 
CTGAAGTGCA AACATGCAGA TGAATTTGGA AGGGCAGAGC AGGAGCAGAT GACCAGAGAG 
CTTGAACTAA TGGACCGTGC TGCCAGTAGC CAACGCAAAC TGGAGACCCT GCTGATAGAA 
ATGGAAGATT CAGAGCTAAA GCATAAGGAA CAAGTCCAAA AACTCCAGCT TGAATTTCAG 
GGTAGGATCC AGGAGCTGGA GCACGCCAAT AGAGAGGAGC TCTGCAAGCT GAAGAAAAGC 
TACAGTCAGA CCATCTGCTT TCTGGAGAAT AAAGGTGAGG CCGATGCGTG TCCAATGGAG 
GAAGGCGAAG GATCTGATTC GGTAACATGC AAGGAGTCCC TGCTCCGAGT CCGGGAGCTG 
GAAATGCAGT TGAGCAACAT GAGAGAGGAG CTAGAACAGA AACCACTGGA TGGAGATCTG 
ACCAGCCTGA GGGAAAAGTA CCAGCGAGAC TTGGACAGTC TCAAGGCCAC ATGCGAGCGT 
GGATTTGCCG CCATGGAGGA GACGCATCAG AAAGTGATTG AAGACATCCA GAGGCAGCAT 
CAGCGGGAGA TCCAGAAACT TCTAGAGGAG AAGGAAAGAC TGCTGGAGGA GGAAACAAAC 
GCCACTATTG CTGCAATTGA GGCCATGAAA AATGCTCATC GTGAGGAACT GGAGAAAACG 
CAGCGGTCGC AGATGAGCGG TGTGAGCGCA GATATTCAGG AGCTGCACAG ACAGTATGAA 
GAGGAGTTGC AGTCTATCCA TCGGGAGCTG GAGGTTTTGT CTGAGCAGTA TTCTCAGAAG 
TGTCTGGAAA ACGCACATTT GGCTCAAGCG CTGGAAGCTG AGAGACAGGC GCTGGGACAG 
TGTCAGCGAG AGAACCAGAA ACTGCATATA CACAACCAGG AACTAAACCA TCGTCTGAAT 
GAGGAGATCA CCAGAATGCA CTCCTGCATC AGCGGAGAAA AAAACACCTC TTCTCTGACG 
CAAGGCAAAG ACATCTATGA GCTGGAGGTT TTGCTACGAG TGAAGGAGTC CGAGATCCAG 
TACCTCAAAC AAGAAATAAA CTCTCTGAAG GACGAGCTGC AGTCGGCACT TCGAGATAAG 
AAGTATGCTT CAGATAAATA CAAGGACATT TACACCGAGC TGAGCATTGT GAAAGCTAAA 
GCTGACTGTG ACATTAACAA GCTGAGGGAG AAACTTTTGG CTGCTACAGA AGCTCTTGGG 
GAACTAGACG CTGAGGGAAG CGGTGTTACG GCTGGATACG ACATTATGAA GTCAAAGAGT 
AACCCAGATT TCCTGAAGAA GGAAAAATCC AGACAGATGC GTGGAGTCAG GTCAAAGAGT 
TTAAAGGAGG GCTTGACCGT ACAGGAGAGG ATGAAGCTCT TCGAAGCGAA AGACTCCAGG 
AAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326086.1
CDS	115..6003
Translation	MCIHTERRHVSQKRVRNRSQMKLNKTKPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_021470411.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470411.1

ORF Insert Sequence:

ATGTGCATAC ACACAGAAAG GCGGCATGTG AGTCAGAAGC GTGTGAGGAA CAGGTCACAG 
ATGAAACTCA ACAAGACTAA GCCTGACCTG CTGAACTTTA AGAAAGGATG GATGACCAAA 
CTATACGAAG ATGGATTGTG GAAGAAACAC TGGTTTGTTC TGACCGATCA GAGTCTGAGG 
TTCTACCGTG ACTCCATTGC TGAGGAGGCT TCTGATCTGG ATGGAGAGAT CGATCTGTCC 
TTGTGTTATG ATGTGACCGA ATTCCCAGTT CAGAGGAACT ACGGCTTCCA AATCCTCAGC 
AAAGAAGGGG CGTTCACACT GTCTGCTATG ACTTCTGGGA TACGTCGTAA CTGGATTCAA 
GCCATTCTGA AAAACATGCG GCCCACTGTC GCTCCTGATG TCACACGGAA AAACGTCTCT 
CTGAAACTTT CAGTTCTTAT GCCCAGTTCC CTTCCGGATG AACGAACCAG ATCAAATGTG 
GAGCAGAGCT TCATCCAACC GCCATCACAG CTCTCCAACA GCTCGATAGT TGAGCCAAAA 
AGGCCACATG AGGACATCAC CGACTCCGCA CCTCCTCCAG ATCAGCGTAA AAGCAGAGGC 
CACAAGCGTG AGGGCCGGTC CAAAACCTTC GACTGGTCAG AATTTCGACC AGGACAAGAA 
AACGAAAGTG GACAACCCTC ACTAAAGAGA GCAGATACTT CAGTCATTAT CTCATCTTCT 
CCATCATCCA TCGCTTCCTC TCCCATCTCT AGCTCTTCTT CTCATCAGAC GTCTTCCTCC 
ATCACGATCA TCAGCCATAC TGAAAAGTTG CCACAGCATA ATGAAGAAGA AGAACAAGTG 
GTGCAGGAGT ATGTACCGCA CCATCAGACT GTACCTACAA CAATGACCGG TCCAGAGACC 
CCGTCAAACC CTCATAATGT GCCACAGGAA CCGGCAGGAA TAGATTTGAA CCAGTCCAGA 
GAAGTGCAGG AGGTCGATGG TCATGGAGTG ATCCGCAAAA ACTCTGATGT CCAAGTGGAA 
ATTGAACAGA GGTGGCATCA GGTGGAAACG ACGCCCCTCA GGGAGGAAAA GCAGGTACCG 
ATCGCTGGTG GCTCACCTAA CGTATCAAAT GTGTCTGTTG GAGAAAGAGC CTCTCCGCAA 
GAGAAGACCA GCAAATTGGA AAAAGAGTTG GGTCAGGCAC AAAAGGAGTT GGCCAGACTC 
CAGGAGCAGA ACAGCATCTT ACAAGACCAA CTTCAGGATG CACGCGGGAG AGAGCAGAAT 
GCACGGGAAG GATATGTACT TCAGAATGAC TCGACTATAG CAAAGCCTTC ATCTGATGCT 
CCCGTGTCTA CCACGGTTCA TAAAGCACCA TGGCAGCGCC TAAGCAAACT AAACCAGGAT 
CTAAAGTCTG AGCTGGAGTC TCAGCGGCGG AAGCAAGATA AGGCCAAACA TCAAGTTAGC 
AGCTTGCGTC GTAGCTACAG TGAAGCAAAA GATATAATAG GGCACCATGA GGCTGAAATC 
GAGGCCTTGG AAGAAAAGCT AACGGCTGCC ATGGCAGAGA TTGTAGTGAG TGAGCAGGCT 
GTAGCACGAA TGCGCAGTGA TCTGAAAATT GAACGCGTTC GTTGTCAAGA GCGTGAGGAA 
GAATGGACAC GAAATGAGAC AACATTACGG TCCCAGCTAC AAGATAGTGA AGAACGTCTT 
CGGCAAGTGG AGGCCAGTTT GCTTGAGAAG AATCAAGAGC TGAGGCAGCT GGAGCAGCAG 
CAAGCTTTAC AAAGGGACCA GCACAGAGAG ATGCAGAGGT TACAGGAGAG ACTCGCTGAG 
GCTACAGGGC GTCTGATTTC CATGGAGGAG TCTCAGACTA TGAGGGAGGA AAGGGAGAGA 
AAAGAGCACC GGAGTCTGGA GGAGAAACAT GAGCGGGAAC GACAAGGGCT GACTCGAAGG 
TTGGCAGAAT CTGAAGAAAG GAGACAAGAA ATAGAAGAGC AATTGCAGGA GGCTCAAGAA 
CAGGTGGAGG CACTGCTGAG GGGCAGCAGT GGGATGGAAA CTGTGGGATT GAGGGAGCAA 
GTGCATCGTC TTCAGCAGGA GTTGGAGACA CAGACTGATA TGGTGGAAAT GCTGCGAGAA 
AGTGTGAGAA GGCTGGAGGA GGAACGTGAC CAGCTTACTT GCCGCTGTCA GGAACTCCTC 
AATCAGATTG CAGAGGCTGA CAGAGAGGTT GGAAAGCAAC AGGCATGTTT AACAACGGTG 
GAAACAGACT TTCACTCCCT TGAGACCTCA TATGAGCATG TATCTGAGGA ATTTGCTAGG 
ATAAGTCATG TGCTACAAGA GAAAGAAGAG GAAGTGAGGC AGACAAAGGA AATGTATGAG 
CATCTTATCA GACAAAAGGA GCAGGACTTG AGTGAAGCCC TCATCAAGAT GGCTGCCTTA 
GGCAGTAGTT TAGAAGAAAC AGAACTTTGC TTGCAGCAAA AAGAAGAACT TCTTTCTAAA 
ATGGAGCGTG ACTATCCAGG ACTGGTAGAG TCCCGAAAAG CAGAACAGGA GCTGCAGGCA 
AAACTGGTAG TTGCAGAGGA TCGAATCGCT GAGCTGGAGG AACACCTCAA TGCCCTGCGA 
CTGGGATATG CAGATCTCAG AATGCGACGT TGTCGCTCTC AGGAAGACAT GCTTGAGAGT 
TTAAAGGAGA TTGAACATGA AGTTTCCCTA TCCTCAAGTA CATCCCAGCT TCTTGCTAGA 
AGCAGTTCAG AGACTGAAAT GTCATTAGTA AAGCGACAGA GGATTCGCTT TTCCACTATC 
CAGTGCCAAA CCTACCACCG GTCCCAGGGA GCAGACAAGT TTCAGACAGA AAACACATCT 
TTGGATCTTA CACAAGATCT GAGTCTAGAC CACATGCATG ACCTGAGCCT GACTCAAGAT 
ATTAGTGTAG TCAGTGACAA TTCATTTCAG CAATGCAGAG ATCCTGAAAA GTTCATCTCC 
ATCATACATG CTCTTGAAAC CAAGCTGTTA GCCACTGAGG AGAAACTCCG GCACCTTACG 
GATAAGATAC GGGACCAGCC CAATCTTTCA GCGGTGCAGG AACATCTCAG TACTAGAGCT 
ACCATGGAGG ACTGGACAAC TAAAACCTGT GATGACTTGC CAATGGATGA ACCCAGCCAG 
AAGCACCTAA ACAAGACTGT CAGTGAACAT GACAGTGGTG AATATGAGAA GGCATTGGCA 
CTGGTAGAGT CTTGCACTGA AAGAGTCAGA GAGATTCTCA GTAATCAGAA TGAGACCAGT 
TCTGCTGAAT CTCTGATTTG TACACTTGCA GAAGTAGAGA AAAGGCTGGT TTGTGCAACT 
GCATTCCTAA GGAAAGGCCA CACATTATAT GAGTCGTGCC ACGTATTTGA TGCACCAGAT 
GTGCAGTCAA TGAAGGGAAG TATTAAGCTG TTTGCCAGAA CATTGTGTTT TGAGGCTGAA 
GTTCTGGAGA AGATGGGGTT CTCTTTGCAG GACTTGAACT CTGATATTAT GGCAGCCCTG 
AACTCCATTC ACAAGGAGGC TGAAAACATC AAGAAGAGCT GTGATGGCTG TCTCTCAGTT 
GTCTATGCTG ATTTGCTTAC AAGGAAACTT CTGCTAGAAA CCATGTTCAT GTCAGAGATA 
GACAAACTTG AGACAGGAAA TCTAGCGAAT AGTGGTTTTT CACAGGATGC CATTAAGAGT 
GCATGCATTA GTGCTGAGCT TGCATATTCA CTTCAGAACC TAACAACGAA TTTCCAGGAA 
AGATTTGATG AGCTTCAAAA GGACTTGATT CAGGCTAACA AGACCTTGAA ACAAAGGGAC 
TTTGCTCTGA AAGATGCTGT TAGTGCATCA AAGCATTCAT GCATTGAAAG CATCCCCCAA 
AGTACGACCG ATCATCTTGT TGGTGATGAT GCCCCTCCTG AACTTGTTCC CTATATGGAG 
CAGATTGAGA TGGAGGAAGC TCAGAGTTTA GCTGCGGAGA TTGTTCGTAG ACATTTAACA 
GGTGGCATGC TATCCCACAG TGCTGAATCA GAATCACATC TCCAAATGGG CTGGGAAAAT 
CTTATTGCTG AGCTGAAGAA GCAAGCCAAA GCTCTTCGCA ATATTTCTCA GGAAATTGAG 
AAGATCTGCG AGACGGGAGA AATGAATTCC CTCTCCAGAC TTGCTGAGGC AATCCAATTA 
AGCTCATACC GCAGCGACTC CAGCTCTGTT TGTATGCGAG AGGCTTTAAT GCAAGTTCAG 
GTCGCCTATG TTTCTTGCAG GTTGAGAGCG GGCCACACAA GGGAACTTTC CCTGTGCCAG 
GAGACCAGCC GCAACATGTC AGCGCTCGTC CAGGAACATG CAGAGAGTGT TGCTGCCATA 
CAAAAGCACT ACCAGAGCTG CTTAGAGAAA GAGCATCTTA GCTTCACAGG CACCATCAGT 
TCCTTGCAGG AGGAGAATGA CATGCTACGA GGGGAGCTAT CCTATAAGCT CAGAGAGCTG 
CAAGAGCAAC GGCAGAACCT GTCCCAGCTG GAAGAAGCAT TTCATAGGGA GAAAGAAGAG 
CTGAAGTGCA AACATGCAGA TGAATTTGGA AGGGCAGAGC AGGAGCAGAT GACCAGAGAG 
CTTGAACTAA TGGACCGTGC TGCCAGTAGC CAACGCAAAC TGGAGACCCT GCTGATAGAA 
ATGGAAGATT CAGAGCTAAA GCATAAGGAA CAAGTCCAAA AACTCCAGCT TGAATTTCAG 
GGTAGGATCC AGGAGCTGGA GCACGCCAAT AGAGAGGAGC TCTGCAAGCT GAAGAAAAGC 
TACAGTCAGA CCATCTGCTT TCTGGAGAAT AAAGGTGAGG CCGATGCGTG TCCAATGGAG 
GAAGGCGAAG GATCTGATTC GGTAACATGC AAGGAGTCCC TGCTCCGAGT CCGGGAGCTG 
GAAATGCAGT TGAGCAACAT GAGAGAGGAG CTAGAACAGA AACCACTGGA TGGAGATCTG 
ACCAGCCTGA GGGAAAAGTA CCAGCGAGAC TTGGACAGTC TCAAGGCCAC ATGCGAGCGT 
GGATTTGCCG CCATGGAGGA GACGCATCAG AAAGTGATTG AAGACATCCA GAGGCAGCAT 
CAGCGGGAGA TCCAGAAACT TCTAGAGGAG AAGGAAAGAC TGCTGGAGGA GGAAACAAAC 
GCCACTATTG CTGCAATTGA GGCCATGAAA AATGCTCATC GTGAGGAACT GGAGAAAACG 
CAGCGGTCGC AGATGAGCGG TGTGAGCGCA GATATTCAGG AGCTGCACAG ACAGTATGAA 
GAGGAGTTGC AGTCTATCCA TCGGGAGCTG GAGGTTTTGT CTGAGCAGTA TTCTCAGAAG 
TGTCTGGAAA ACGCACATTT GGCTCAAGCG CTGGAAGCTG AGAGACAGGC GCTGGGACAG 
TGTCAGCGAG AGAACCAGAA ACTGCATATA CACAACCAGG AACTAAACCA TCGTCTGAAT 
GAGGAGATCA CCAGAATGCA CTCCTGCATC AGCGGAGAAA AAAACACCTC TTCTCTGACG 
CAAGGCAAAG ACATCTATGA GCTGGAGGTT TTGCTACGAG TGAAGGAGTC CGAGATCCAG 
TACCTCAAAC AAGAAATAAA CTCTCTGAAG GACGAGCTGC AGTCGGCACT TCGAGATAAG 
AAGTATGCTT CAGATAAATA CAAGGACATT TACACCGAGC TGAGCATTGT GAAAGCTAAA 
GCTGACTGTG ACATTAACAA GCTGAGGGAG AAACTTTTGG CTGCTACAGA AGCTCTTGGG 
GAACTAGACG CTGAGGGAAG CGGTGTTACG GCTGGATACG ACATTATGAA GTCAAAGAGT 
AACCCAGATT TCCTGAAGAA GGAAAAATCC AGACAGATGC GTGGAGTCAG GTCAAAGAGT 
TTAAAGGAGG GCTTGACCGT ACAGGAGAGG ATGAAGCTCT TCGAAGCGAA AGACTCCAGG 
AAGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162900.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162957.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X1, mRNA.

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CloneID	ODa28901
Clone ID Related Accession (Same CDS sequence)	XM_005162900.3 , XM_005162900.4
Accession Version	XM_005162900.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centrosome-associated protein CEP250 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162900.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GAGTTTAAAG 
GAGGGCTTGA CCGTACAGGA GAGGATGAAG CTCTTCGAAG CGAAAGACTC CAGGAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162957.1
CDS	627..8069
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162900.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162900.3

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GAGTTTAAAG 
GAGGGCTTGA CCGTACAGGA GAGGATGAAG CTCTTCGAAG CGAAAGACTC CAGGAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162900.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162957.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X1, mRNA.

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CloneID	ODa28901
Clone ID Related Accession (Same CDS sequence)	XM_005162900.3 , XM_005162900.4
Accession Version	XM_005162900.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162900.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GAGTTTAAAG 
GAGGGCTTGA CCGTACAGGA GAGGATGAAG CTCTTCGAAG CGAAAGACTC CAGGAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162957.1
CDS	635..8077
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162900.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162900.4

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GAGTTTAAAG 
GAGGGCTTGA CCGTACAGGA GAGGATGAAG CTCTTCGAAG CGAAAGACTC CAGGAAGATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162901.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162958.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X2, mRNA.

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CloneID	ODa28902
Clone ID Related Accession (Same CDS sequence)	XM_005162901.3 , XM_005162901.4
Accession Version	XM_005162901.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centrosome-associated protein CEP250 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GTTCCCTTCC GGATGAACGA 
ACCAGATCAA ATGTGGAGCA GAGCTTCATC CAACCGCCAT CACAGCTCTC CAACAGCTCG 
ATAGTTGAGC CAAAAAGGCC ACATGAGGAC ATCACCGACT CCGCACCTCC TCCAGATCAG 
CGTAAAAGCA GAGGCCACAA GCGTGAGGGC CGGTCCAAAA CCTTCGACTG GTCAGAATTT 
CGACCAGGAC AAGAAAACGA AAGTGGACAA CCCTCACTAA AGAGAGCAGA TACTTCAGTC 
ATTATCTCAT CTTCTCCATC ATCCATCGCT TCCTCTCCCA TCTCTAGCTC TTCTTCTCAT 
CAGACGTCTT CCTCCATCAC GATCATCAGC CATACTGAAA AGTTGCCACA GCATAATGAA 
GAAGAAGAAC AAGTGGTGCA GGAGTATGTA CCGCACCATC AGACTGTACC TACAACAATG 
ACCGGTCCAG AGACCCCGTC AAACCCTCAT AATGTGCCAC AGGAACCGGC AGGAATAGAT 
TTGAACCAGT CCAGAGAAGT GCAGGAGGTC GATGGTCATG GAGTGATCCG CAAAAACTCT 
GATGTCCAAG TGGAAATTGA ACAGAGGTGG CATCAGGTGG AAACGACGCC CCTCAGGGAG 
GAAAAGCAGG TACCGATCGC TGGTGGCTCA CCTAACGTAT CAAATGTGTC TGTTGGAGAA 
AGAGCCTCTC CGCAAGAGAA GACCAGCAAA TTGGAAAAAG AGTTGGGTCA GGCACAAAAG 
GAGTTGGCCA GACTCCAGGA GCAGAACAGC ATCTTACAAG ACCAACTTCA GGATGCACGC 
GGGAGAGAGC AGAATGCACG GGAAGGATAT GTACTTCAGA ATGACTCGAC TATAGCAAAG 
CCTTCATCTG ATGCTCCCGT GTCTACCACG GTTCATAAAG CACCATGGCA GCGCCTAAGC 
AAACTAAACC AGGATCTAAA GTCTGAGCTG GAGTCTCAGC GGCGGAAGCA AGATAAGGCC 
AAACATCAAG TTAGCAGCTT GCGTCGTAGC TACAGTGAAG CAAAAGATAT AATAGGGCAC 
CATGAGGCTG AAATCGAGGC CTTGGAAGAA AAGCTAACGG CTGCCATGGC AGAGATTGTA 
GTGAGTGAGC AGGCTGTAGC ACGAATGCGC AGTGATCTGA AAATTGAACG CGTTCGTTGT 
CAAGAGCGTG AGGAAGAATG GACACGAAAT GAGACAACAT TACGGTCCCA GCTACAAGAT 
AGTGAAGAAC GTCTTCGGCA AGTGGAGGCC AGTTTGCTTG AGAAGAATCA AGAGCTGAGG 
CAGCTGGAGC AGCAGCAAGC TTTACAAAGG GACCAGCACA GAGAGATGCA GAGGTTACAG 
GAGAGACTCG CTGAGGCTAC AGGGCGTCTG ATTTCCATGG AGGAGTCTCA GACTATGAGG 
GAGGAAAGGG AGAGAAAAGA GCACCGGAGT CTGGAGGAGA AACATGAGCG GGAACGACAA 
GGGCTGACTC GAAGGTTGGC AGAATCTGAA GAAAGGAGAC AAGAAATAGA AGAGCAATTG 
CAGGAGGCTC AAGAACAGGT GGAGGCACTG CTGAGGGGCA GCAGTGGGAT GGAAACTGTG 
GGATTGAGGG AGCAAGTGCA TCGTCTTCAG CAGGAGTTGG AGACACAGAC TGATATGGTG 
GAAATGCTGC GAGAAAGTGT GAGAAGGCTG GAGGAGGAAC GTGACCAGCT TACTTGCCGC 
TGTCAGGAAC TCCTCAATCA GATTGCAGAG GCTGACAGAG AGGTTGGAAA GCAACAGGCA 
TGTTTAACAA CGGTGGAAAC AGACTTTCAC TCCCTTGAGA CCTCATATGA GCATGTATCT 
GAGGAATTTG CTAGGATAAG TCATGTGCTA CAAGAGAAAG AAGAGGAAGT GAGGCAGACA 
AAGGAAATGT ATGAGCATCT TATCAGACAA AAGGAGCAGG ACTTGAGTGA AGCCCTCATC 
AAGATGGCTG CCTTAGGCAG TAGTTTAGAA GAAACAGAAC TTTGCTTGCA GCAAAAAGAA 
GAACTTCTTT CTAAAATGGA GCGTGACTAT CCAGGACTGG TAGAGTCCCG AAAAGCAGAA 
CAGGAGCTGC AGGCAAAACT GGTAGTTGCA GAGGATCGAA TCGCTGAGCT GGAGGAACAC 
CTCAATGCCC TGCGACTGGG ATATGCAGAT CTCAGAATGC GACGTTGTCG CTCTCAGGAA 
GACATGCTTG AGAGTTTAAA GGAGATTGAA CATGAAGTTT CCCTATCCTC AAGTACATCC 
CAGCTTCTTG CTAGAAGCAG TTCAGAGACT GAAATGTCAT TAGTAAAGCG ACAGAGGATT 
CGCTTTTCCA CTATCCAGTG CCAAACCTAC CACCGGTCCC AGGGAGCAGA CAAGTTTCAG 
ACAGAAAACA CATCTTTGGA TCTTACACAA GATCTGAGTC TAGACCACAT GCATGACCTG 
AGCCTGACTC AAGATATTAG TGTAGTCAGT GACAATTCAT TTCAGCAATG CAGAGATCCT 
GAAAAGTTCA TCTCCATCAT ACATGCTCTT GAAACCAAGC TGTTAGCCAC TGAGGAGAAA 
CTCCGGCACC TTACGGATAA GATACGGGAC CAGCCCAATC TTTCAGCGGT GCAGGAACAT 
CTCAGTACTA GAGCTACCAT GGAGGACTGG ACAACTAAAA CCTGTGATGA CTTGCCAATG 
GATGAACCCA GCCAGAAGCA CCTAAACAAG ACTGTCAGTG AACATGACAG TGGTGAATAT 
GAGAAGGCAT TGGCACTGGT AGAGTCTTGC ACTGAAAGAG TCAGAGAGAT TCTCAGTAAT 
CAGAATGAGA CCAGTTCTGC TGAATCTCTG ATTTGTACAC TTGCAGAAGT AGAGAAAAGG 
CTGGTTTGTG CAACTGCATT CCTAAGGAAA GGCCACACAT TATATGAGTC GTGCCACGTA 
TTTGATGCAC CAGATGTGCA GTCAATGAAG GGAAGTATTA AGCTGTTTGC CAGAACATTG 
TGTTTTGAGG CTGAAGTTCT GGAGAAGATG GGGTTCTCTT TGCAGGACTT GAACTCTGAT 
ATTATGGCAG CCCTGAACTC CATTCACAAG GAGGCTGAAA ACATCAAGAA GAGCTGTGAT 
GGCTGTCTCT CAGTTGTCTA TGCTGATTTG CTTACAAGGA AACTTCTGCT AGAAACCATG 
TTCATGTCAG AGATAGACAA ACTTGAGACA GGAAATCTAG CGAATAGTGG TTTTTCACAG 
GATGCCATTA AGAGTGCATG CATTAGTGCT GAGCTTGCAT ATTCACTTCA GAACCTAACA 
ACGAATTTCC AGGAAAGATT TGATGAGCTT CAAAAGGACT TGATTCAGGC TAACAAGACC 
TTGAAACAAA GGGACTTTGC TCTGAAAGAT GCTGTTAGTG CATCAAAGCA TTCATGCATT 
GAAAGCATCC CCCAAAGTAC GACCGATCAT CTTGTTGGTG ATGATGCCCC TCCTGAACTT 
GTTCCCTATA TGGAGCAGAT TGAGATGGAG GAAGCTCAGA GTTTAGCTGC GGAGATTGTT 
CGTAGACATT TAACAGGTGG CATGCTATCC CACAGTGCTG AATCAGAATC ACATCTCCAA 
ATGGGCTGGG AAAATCTTAT TGCTGAGCTG AAGAAGCAAG CCAAAGCTCT TCGCAATATT 
TCTCAGGAAA TTGAGAAGAT CTGCGAGACG GGAGAAATGA ATTCCCTCTC CAGACTTGCT 
GAGGCAATCC AATTAAGCTC ATACCGCAGC GACTCCAGCT CTGTTTGTAT GCGAGAGGCT 
TTAATGCAAG TTCAGGTCGC CTATGTTTCT TGCAGGTTGA GAGCGGGCCA CACAAGGGAA 
CTTTCCCTGT GCCAGGAGAC CAGCCGCAAC ATGTCAGCGC TCGTCCAGGA ACATGCAGAG 
AGTGTTGCTG CCATACAAAA GCACTACCAG AGCTGCTTAG AGAAAGAGCA TCTTAGCTTC 
ACAGGCACCA TCAGTTCCTT GCAGGAGGAG AATGACATGC TACGAGGGGA GCTATCCTAT 
AAGCTCAGAG AGCTGCAAGA GCAACGGCAG AACCTGTCCC AGCTGGAAGA AGCATTTCAT 
AGGGAGAAAG AAGAGCTGAA GTGCAAACAT GCAGATGAAT TTGGAAGGGC AGAGCAGGAG 
CAGATGACCA GAGAGCTTGA ACTAATGGAC CGTGCTGCCA GTAGCCAACG CAAACTGGAG 
ACCCTGCTGA TAGAAATGGA AGATTCAGAG CTAAAGCATA AGGAACAAGT CCAAAAACTC 
CAGCTTGAAT TTCAGGGTAG GATCCAGGAG CTGGAGCACG CCAATAGAGA GGAGCTCTGC 
AAGCTGAAGA AAAGCTACAG TCAGACCATC TGCTTTCTGG AGAATAAAGG TGAGGCCGAT 
GCGTGTCCAA TGGAGGAAGG CGAAGGATCT GATTCGGTAA CATGCAAGGA GTCCCTGCTC 
CGAGTCCGGG AGCTGGAAAT GCAGTTGAGC AACATGAGAG AGGAGCTAGA ACAGAAACCA 
CTGGATGGAG ATCTGACCAG CCTGAGGGAA AAGTACCAGC GAGACTTGGA CAGTCTCAAG 
GCCACATGCG AGCGTGGATT TGCCGCCATG GAGGAGACGC ATCAGAAAGT GATTGAAGAC 
ATCCAGAGGC AGCATCAGCG GGAGATCCAG AAACTTCTAG AGGAGAAGGA AAGACTGCTG 
GAGGAGGAAA CAAACGCCAC TATTGCTGCA ATTGAGGCCA TGAAAAATGC TCATCGTGAG 
GAACTGGAGA AAACGCAGCG GTCGCAGATG AGCGGTGTGA GCGCAGATAT TCAGGAGCTG 
CACAGACAGT ATGAAGAGGA GTTGCAGTCT ATCCATCGGG AGCTGGAGGT TTTGTCTGAG 
CAGTATTCTC AGAAGTGTCT GGAAAACGCA CATTTGGCTC AAGCGCTGGA AGCTGAGAGA 
CAGGCGCTGG GACAGTGTCA GCGAGAGAAC CAGAAACTGC ATATACACAA CCAGGAACTA 
AACCATCGTC TGAATGAGGA GATCACCAGA ATGCACTCCT GCATCAGCGG AGAAAAAAAC 
ACCTCTTCTC TGACGCAAGG CAAAGACATC TATGAGCTGG AGGTTTTGCT ACGAGTGAAG 
GAGTCCGAGA TCCAGTACCT CAAACAAGAA ATAAACTCTC TGAAGGACGA GCTGCAGTCG 
GCACTTCGAG ATAAGAAGTA TGCTTCAGAT AAATACAAGG ACATTTACAC CGAGCTGAGC 
ATTGTGAAAG CTAAAGCTGA CTGTGACATT AACAAGCTGA GGGAGAAACT TTTGGCTGCT 
ACAGAAGCTC TTGGGGAACT AGACGCTGAG GGAAGCGGTG TTACGGCTGG ATACGACATT 
ATGAAGTCAA AGAGTAACCC AGATTTCCTG AAGAAGGAAA AATCCAGACA GATGCGTGGA 
GTCAGGTCAA AGAGTTTAAA GGAGGGCTTG ACCGTACAGG AGAGGATGAA GCTCTTCGAA 
GCGAAAGACT CCAGGAAGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162958.1
CDS	627..8030
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162901.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162901.3

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GTTCCCTTCC GGATGAACGA 
ACCAGATCAA ATGTGGAGCA GAGCTTCATC CAACCGCCAT CACAGCTCTC CAACAGCTCG 
ATAGTTGAGC CAAAAAGGCC ACATGAGGAC ATCACCGACT CCGCACCTCC TCCAGATCAG 
CGTAAAAGCA GAGGCCACAA GCGTGAGGGC CGGTCCAAAA CCTTCGACTG GTCAGAATTT 
CGACCAGGAC AAGAAAACGA AAGTGGACAA CCCTCACTAA AGAGAGCAGA TACTTCAGTC 
ATTATCTCAT CTTCTCCATC ATCCATCGCT TCCTCTCCCA TCTCTAGCTC TTCTTCTCAT 
CAGACGTCTT CCTCCATCAC GATCATCAGC CATACTGAAA AGTTGCCACA GCATAATGAA 
GAAGAAGAAC AAGTGGTGCA GGAGTATGTA CCGCACCATC AGACTGTACC TACAACAATG 
ACCGGTCCAG AGACCCCGTC AAACCCTCAT AATGTGCCAC AGGAACCGGC AGGAATAGAT 
TTGAACCAGT CCAGAGAAGT GCAGGAGGTC GATGGTCATG GAGTGATCCG CAAAAACTCT 
GATGTCCAAG TGGAAATTGA ACAGAGGTGG CATCAGGTGG AAACGACGCC CCTCAGGGAG 
GAAAAGCAGG TACCGATCGC TGGTGGCTCA CCTAACGTAT CAAATGTGTC TGTTGGAGAA 
AGAGCCTCTC CGCAAGAGAA GACCAGCAAA TTGGAAAAAG AGTTGGGTCA GGCACAAAAG 
GAGTTGGCCA GACTCCAGGA GCAGAACAGC ATCTTACAAG ACCAACTTCA GGATGCACGC 
GGGAGAGAGC AGAATGCACG GGAAGGATAT GTACTTCAGA ATGACTCGAC TATAGCAAAG 
CCTTCATCTG ATGCTCCCGT GTCTACCACG GTTCATAAAG CACCATGGCA GCGCCTAAGC 
AAACTAAACC AGGATCTAAA GTCTGAGCTG GAGTCTCAGC GGCGGAAGCA AGATAAGGCC 
AAACATCAAG TTAGCAGCTT GCGTCGTAGC TACAGTGAAG CAAAAGATAT AATAGGGCAC 
CATGAGGCTG AAATCGAGGC CTTGGAAGAA AAGCTAACGG CTGCCATGGC AGAGATTGTA 
GTGAGTGAGC AGGCTGTAGC ACGAATGCGC AGTGATCTGA AAATTGAACG CGTTCGTTGT 
CAAGAGCGTG AGGAAGAATG GACACGAAAT GAGACAACAT TACGGTCCCA GCTACAAGAT 
AGTGAAGAAC GTCTTCGGCA AGTGGAGGCC AGTTTGCTTG AGAAGAATCA AGAGCTGAGG 
CAGCTGGAGC AGCAGCAAGC TTTACAAAGG GACCAGCACA GAGAGATGCA GAGGTTACAG 
GAGAGACTCG CTGAGGCTAC AGGGCGTCTG ATTTCCATGG AGGAGTCTCA GACTATGAGG 
GAGGAAAGGG AGAGAAAAGA GCACCGGAGT CTGGAGGAGA AACATGAGCG GGAACGACAA 
GGGCTGACTC GAAGGTTGGC AGAATCTGAA GAAAGGAGAC AAGAAATAGA AGAGCAATTG 
CAGGAGGCTC AAGAACAGGT GGAGGCACTG CTGAGGGGCA GCAGTGGGAT GGAAACTGTG 
GGATTGAGGG AGCAAGTGCA TCGTCTTCAG CAGGAGTTGG AGACACAGAC TGATATGGTG 
GAAATGCTGC GAGAAAGTGT GAGAAGGCTG GAGGAGGAAC GTGACCAGCT TACTTGCCGC 
TGTCAGGAAC TCCTCAATCA GATTGCAGAG GCTGACAGAG AGGTTGGAAA GCAACAGGCA 
TGTTTAACAA CGGTGGAAAC AGACTTTCAC TCCCTTGAGA CCTCATATGA GCATGTATCT 
GAGGAATTTG CTAGGATAAG TCATGTGCTA CAAGAGAAAG AAGAGGAAGT GAGGCAGACA 
AAGGAAATGT ATGAGCATCT TATCAGACAA AAGGAGCAGG ACTTGAGTGA AGCCCTCATC 
AAGATGGCTG CCTTAGGCAG TAGTTTAGAA GAAACAGAAC TTTGCTTGCA GCAAAAAGAA 
GAACTTCTTT CTAAAATGGA GCGTGACTAT CCAGGACTGG TAGAGTCCCG AAAAGCAGAA 
CAGGAGCTGC AGGCAAAACT GGTAGTTGCA GAGGATCGAA TCGCTGAGCT GGAGGAACAC 
CTCAATGCCC TGCGACTGGG ATATGCAGAT CTCAGAATGC GACGTTGTCG CTCTCAGGAA 
GACATGCTTG AGAGTTTAAA GGAGATTGAA CATGAAGTTT CCCTATCCTC AAGTACATCC 
CAGCTTCTTG CTAGAAGCAG TTCAGAGACT GAAATGTCAT TAGTAAAGCG ACAGAGGATT 
CGCTTTTCCA CTATCCAGTG CCAAACCTAC CACCGGTCCC AGGGAGCAGA CAAGTTTCAG 
ACAGAAAACA CATCTTTGGA TCTTACACAA GATCTGAGTC TAGACCACAT GCATGACCTG 
AGCCTGACTC AAGATATTAG TGTAGTCAGT GACAATTCAT TTCAGCAATG CAGAGATCCT 
GAAAAGTTCA TCTCCATCAT ACATGCTCTT GAAACCAAGC TGTTAGCCAC TGAGGAGAAA 
CTCCGGCACC TTACGGATAA GATACGGGAC CAGCCCAATC TTTCAGCGGT GCAGGAACAT 
CTCAGTACTA GAGCTACCAT GGAGGACTGG ACAACTAAAA CCTGTGATGA CTTGCCAATG 
GATGAACCCA GCCAGAAGCA CCTAAACAAG ACTGTCAGTG AACATGACAG TGGTGAATAT 
GAGAAGGCAT TGGCACTGGT AGAGTCTTGC ACTGAAAGAG TCAGAGAGAT TCTCAGTAAT 
CAGAATGAGA CCAGTTCTGC TGAATCTCTG ATTTGTACAC TTGCAGAAGT AGAGAAAAGG 
CTGGTTTGTG CAACTGCATT CCTAAGGAAA GGCCACACAT TATATGAGTC GTGCCACGTA 
TTTGATGCAC CAGATGTGCA GTCAATGAAG GGAAGTATTA AGCTGTTTGC CAGAACATTG 
TGTTTTGAGG CTGAAGTTCT GGAGAAGATG GGGTTCTCTT TGCAGGACTT GAACTCTGAT 
ATTATGGCAG CCCTGAACTC CATTCACAAG GAGGCTGAAA ACATCAAGAA GAGCTGTGAT 
GGCTGTCTCT CAGTTGTCTA TGCTGATTTG CTTACAAGGA AACTTCTGCT AGAAACCATG 
TTCATGTCAG AGATAGACAA ACTTGAGACA GGAAATCTAG CGAATAGTGG TTTTTCACAG 
GATGCCATTA AGAGTGCATG CATTAGTGCT GAGCTTGCAT ATTCACTTCA GAACCTAACA 
ACGAATTTCC AGGAAAGATT TGATGAGCTT CAAAAGGACT TGATTCAGGC TAACAAGACC 
TTGAAACAAA GGGACTTTGC TCTGAAAGAT GCTGTTAGTG CATCAAAGCA TTCATGCATT 
GAAAGCATCC CCCAAAGTAC GACCGATCAT CTTGTTGGTG ATGATGCCCC TCCTGAACTT 
GTTCCCTATA TGGAGCAGAT TGAGATGGAG GAAGCTCAGA GTTTAGCTGC GGAGATTGTT 
CGTAGACATT TAACAGGTGG CATGCTATCC CACAGTGCTG AATCAGAATC ACATCTCCAA 
ATGGGCTGGG AAAATCTTAT TGCTGAGCTG AAGAAGCAAG CCAAAGCTCT TCGCAATATT 
TCTCAGGAAA TTGAGAAGAT CTGCGAGACG GGAGAAATGA ATTCCCTCTC CAGACTTGCT 
GAGGCAATCC AATTAAGCTC ATACCGCAGC GACTCCAGCT CTGTTTGTAT GCGAGAGGCT 
TTAATGCAAG TTCAGGTCGC CTATGTTTCT TGCAGGTTGA GAGCGGGCCA CACAAGGGAA 
CTTTCCCTGT GCCAGGAGAC CAGCCGCAAC ATGTCAGCGC TCGTCCAGGA ACATGCAGAG 
AGTGTTGCTG CCATACAAAA GCACTACCAG AGCTGCTTAG AGAAAGAGCA TCTTAGCTTC 
ACAGGCACCA TCAGTTCCTT GCAGGAGGAG AATGACATGC TACGAGGGGA GCTATCCTAT 
AAGCTCAGAG AGCTGCAAGA GCAACGGCAG AACCTGTCCC AGCTGGAAGA AGCATTTCAT 
AGGGAGAAAG AAGAGCTGAA GTGCAAACAT GCAGATGAAT TTGGAAGGGC AGAGCAGGAG 
CAGATGACCA GAGAGCTTGA ACTAATGGAC CGTGCTGCCA GTAGCCAACG CAAACTGGAG 
ACCCTGCTGA TAGAAATGGA AGATTCAGAG CTAAAGCATA AGGAACAAGT CCAAAAACTC 
CAGCTTGAAT TTCAGGGTAG GATCCAGGAG CTGGAGCACG CCAATAGAGA GGAGCTCTGC 
AAGCTGAAGA AAAGCTACAG TCAGACCATC TGCTTTCTGG AGAATAAAGG TGAGGCCGAT 
GCGTGTCCAA TGGAGGAAGG CGAAGGATCT GATTCGGTAA CATGCAAGGA GTCCCTGCTC 
CGAGTCCGGG AGCTGGAAAT GCAGTTGAGC AACATGAGAG AGGAGCTAGA ACAGAAACCA 
CTGGATGGAG ATCTGACCAG CCTGAGGGAA AAGTACCAGC GAGACTTGGA CAGTCTCAAG 
GCCACATGCG AGCGTGGATT TGCCGCCATG GAGGAGACGC ATCAGAAAGT GATTGAAGAC 
ATCCAGAGGC AGCATCAGCG GGAGATCCAG AAACTTCTAG AGGAGAAGGA AAGACTGCTG 
GAGGAGGAAA CAAACGCCAC TATTGCTGCA ATTGAGGCCA TGAAAAATGC TCATCGTGAG 
GAACTGGAGA AAACGCAGCG GTCGCAGATG AGCGGTGTGA GCGCAGATAT TCAGGAGCTG 
CACAGACAGT ATGAAGAGGA GTTGCAGTCT ATCCATCGGG AGCTGGAGGT TTTGTCTGAG 
CAGTATTCTC AGAAGTGTCT GGAAAACGCA CATTTGGCTC AAGCGCTGGA AGCTGAGAGA 
CAGGCGCTGG GACAGTGTCA GCGAGAGAAC CAGAAACTGC ATATACACAA CCAGGAACTA 
AACCATCGTC TGAATGAGGA GATCACCAGA ATGCACTCCT GCATCAGCGG AGAAAAAAAC 
ACCTCTTCTC TGACGCAAGG CAAAGACATC TATGAGCTGG AGGTTTTGCT ACGAGTGAAG 
GAGTCCGAGA TCCAGTACCT CAAACAAGAA ATAAACTCTC TGAAGGACGA GCTGCAGTCG 
GCACTTCGAG ATAAGAAGTA TGCTTCAGAT AAATACAAGG ACATTTACAC CGAGCTGAGC 
ATTGTGAAAG CTAAAGCTGA CTGTGACATT AACAAGCTGA GGGAGAAACT TTTGGCTGCT 
ACAGAAGCTC TTGGGGAACT AGACGCTGAG GGAAGCGGTG TTACGGCTGG ATACGACATT 
ATGAAGTCAA AGAGTAACCC AGATTTCCTG AAGAAGGAAA AATCCAGACA GATGCGTGGA 
GTCAGGTCAA AGAGTTTAAA GGAGGGCTTG ACCGTACAGG AGAGGATGAA GCTCTTCGAA 
GCGAAAGACT CCAGGAAGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162901.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162958.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X2, mRNA.

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CloneID	ODa28902
Clone ID Related Accession (Same CDS sequence)	XM_005162901.3 , XM_005162901.4
Accession Version	XM_005162901.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162901.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GTTCCCTTCC GGATGAACGA 
ACCAGATCAA ATGTGGAGCA GAGCTTCATC CAACCGCCAT CACAGCTCTC CAACAGCTCG 
ATAGTTGAGC CAAAAAGGCC ACATGAGGAC ATCACCGACT CCGCACCTCC TCCAGATCAG 
CGTAAAAGCA GAGGCCACAA GCGTGAGGGC CGGTCCAAAA CCTTCGACTG GTCAGAATTT 
CGACCAGGAC AAGAAAACGA AAGTGGACAA CCCTCACTAA AGAGAGCAGA TACTTCAGTC 
ATTATCTCAT CTTCTCCATC ATCCATCGCT TCCTCTCCCA TCTCTAGCTC TTCTTCTCAT 
CAGACGTCTT CCTCCATCAC GATCATCAGC CATACTGAAA AGTTGCCACA GCATAATGAA 
GAAGAAGAAC AAGTGGTGCA GGAGTATGTA CCGCACCATC AGACTGTACC TACAACAATG 
ACCGGTCCAG AGACCCCGTC AAACCCTCAT AATGTGCCAC AGGAACCGGC AGGAATAGAT 
TTGAACCAGT CCAGAGAAGT GCAGGAGGTC GATGGTCATG GAGTGATCCG CAAAAACTCT 
GATGTCCAAG TGGAAATTGA ACAGAGGTGG CATCAGGTGG AAACGACGCC CCTCAGGGAG 
GAAAAGCAGG TACCGATCGC TGGTGGCTCA CCTAACGTAT CAAATGTGTC TGTTGGAGAA 
AGAGCCTCTC CGCAAGAGAA GACCAGCAAA TTGGAAAAAG AGTTGGGTCA GGCACAAAAG 
GAGTTGGCCA GACTCCAGGA GCAGAACAGC ATCTTACAAG ACCAACTTCA GGATGCACGC 
GGGAGAGAGC AGAATGCACG GGAAGGATAT GTACTTCAGA ATGACTCGAC TATAGCAAAG 
CCTTCATCTG ATGCTCCCGT GTCTACCACG GTTCATAAAG CACCATGGCA GCGCCTAAGC 
AAACTAAACC AGGATCTAAA GTCTGAGCTG GAGTCTCAGC GGCGGAAGCA AGATAAGGCC 
AAACATCAAG TTAGCAGCTT GCGTCGTAGC TACAGTGAAG CAAAAGATAT AATAGGGCAC 
CATGAGGCTG AAATCGAGGC CTTGGAAGAA AAGCTAACGG CTGCCATGGC AGAGATTGTA 
GTGAGTGAGC AGGCTGTAGC ACGAATGCGC AGTGATCTGA AAATTGAACG CGTTCGTTGT 
CAAGAGCGTG AGGAAGAATG GACACGAAAT GAGACAACAT TACGGTCCCA GCTACAAGAT 
AGTGAAGAAC GTCTTCGGCA AGTGGAGGCC AGTTTGCTTG AGAAGAATCA AGAGCTGAGG 
CAGCTGGAGC AGCAGCAAGC TTTACAAAGG GACCAGCACA GAGAGATGCA GAGGTTACAG 
GAGAGACTCG CTGAGGCTAC AGGGCGTCTG ATTTCCATGG AGGAGTCTCA GACTATGAGG 
GAGGAAAGGG AGAGAAAAGA GCACCGGAGT CTGGAGGAGA AACATGAGCG GGAACGACAA 
GGGCTGACTC GAAGGTTGGC AGAATCTGAA GAAAGGAGAC AAGAAATAGA AGAGCAATTG 
CAGGAGGCTC AAGAACAGGT GGAGGCACTG CTGAGGGGCA GCAGTGGGAT GGAAACTGTG 
GGATTGAGGG AGCAAGTGCA TCGTCTTCAG CAGGAGTTGG AGACACAGAC TGATATGGTG 
GAAATGCTGC GAGAAAGTGT GAGAAGGCTG GAGGAGGAAC GTGACCAGCT TACTTGCCGC 
TGTCAGGAAC TCCTCAATCA GATTGCAGAG GCTGACAGAG AGGTTGGAAA GCAACAGGCA 
TGTTTAACAA CGGTGGAAAC AGACTTTCAC TCCCTTGAGA CCTCATATGA GCATGTATCT 
GAGGAATTTG CTAGGATAAG TCATGTGCTA CAAGAGAAAG AAGAGGAAGT GAGGCAGACA 
AAGGAAATGT ATGAGCATCT TATCAGACAA AAGGAGCAGG ACTTGAGTGA AGCCCTCATC 
AAGATGGCTG CCTTAGGCAG TAGTTTAGAA GAAACAGAAC TTTGCTTGCA GCAAAAAGAA 
GAACTTCTTT CTAAAATGGA GCGTGACTAT CCAGGACTGG TAGAGTCCCG AAAAGCAGAA 
CAGGAGCTGC AGGCAAAACT GGTAGTTGCA GAGGATCGAA TCGCTGAGCT GGAGGAACAC 
CTCAATGCCC TGCGACTGGG ATATGCAGAT CTCAGAATGC GACGTTGTCG CTCTCAGGAA 
GACATGCTTG AGAGTTTAAA GGAGATTGAA CATGAAGTTT CCCTATCCTC AAGTACATCC 
CAGCTTCTTG CTAGAAGCAG TTCAGAGACT GAAATGTCAT TAGTAAAGCG ACAGAGGATT 
CGCTTTTCCA CTATCCAGTG CCAAACCTAC CACCGGTCCC AGGGAGCAGA CAAGTTTCAG 
ACAGAAAACA CATCTTTGGA TCTTACACAA GATCTGAGTC TAGACCACAT GCATGACCTG 
AGCCTGACTC AAGATATTAG TGTAGTCAGT GACAATTCAT TTCAGCAATG CAGAGATCCT 
GAAAAGTTCA TCTCCATCAT ACATGCTCTT GAAACCAAGC TGTTAGCCAC TGAGGAGAAA 
CTCCGGCACC TTACGGATAA GATACGGGAC CAGCCCAATC TTTCAGCGGT GCAGGAACAT 
CTCAGTACTA GAGCTACCAT GGAGGACTGG ACAACTAAAA CCTGTGATGA CTTGCCAATG 
GATGAACCCA GCCAGAAGCA CCTAAACAAG ACTGTCAGTG AACATGACAG TGGTGAATAT 
GAGAAGGCAT TGGCACTGGT AGAGTCTTGC ACTGAAAGAG TCAGAGAGAT TCTCAGTAAT 
CAGAATGAGA CCAGTTCTGC TGAATCTCTG ATTTGTACAC TTGCAGAAGT AGAGAAAAGG 
CTGGTTTGTG CAACTGCATT CCTAAGGAAA GGCCACACAT TATATGAGTC GTGCCACGTA 
TTTGATGCAC CAGATGTGCA GTCAATGAAG GGAAGTATTA AGCTGTTTGC CAGAACATTG 
TGTTTTGAGG CTGAAGTTCT GGAGAAGATG GGGTTCTCTT TGCAGGACTT GAACTCTGAT 
ATTATGGCAG CCCTGAACTC CATTCACAAG GAGGCTGAAA ACATCAAGAA GAGCTGTGAT 
GGCTGTCTCT CAGTTGTCTA TGCTGATTTG CTTACAAGGA AACTTCTGCT AGAAACCATG 
TTCATGTCAG AGATAGACAA ACTTGAGACA GGAAATCTAG CGAATAGTGG TTTTTCACAG 
GATGCCATTA AGAGTGCATG CATTAGTGCT GAGCTTGCAT ATTCACTTCA GAACCTAACA 
ACGAATTTCC AGGAAAGATT TGATGAGCTT CAAAAGGACT TGATTCAGGC TAACAAGACC 
TTGAAACAAA GGGACTTTGC TCTGAAAGAT GCTGTTAGTG CATCAAAGCA TTCATGCATT 
GAAAGCATCC CCCAAAGTAC GACCGATCAT CTTGTTGGTG ATGATGCCCC TCCTGAACTT 
GTTCCCTATA TGGAGCAGAT TGAGATGGAG GAAGCTCAGA GTTTAGCTGC GGAGATTGTT 
CGTAGACATT TAACAGGTGG CATGCTATCC CACAGTGCTG AATCAGAATC ACATCTCCAA 
ATGGGCTGGG AAAATCTTAT TGCTGAGCTG AAGAAGCAAG CCAAAGCTCT TCGCAATATT 
TCTCAGGAAA TTGAGAAGAT CTGCGAGACG GGAGAAATGA ATTCCCTCTC CAGACTTGCT 
GAGGCAATCC AATTAAGCTC ATACCGCAGC GACTCCAGCT CTGTTTGTAT GCGAGAGGCT 
TTAATGCAAG TTCAGGTCGC CTATGTTTCT TGCAGGTTGA GAGCGGGCCA CACAAGGGAA 
CTTTCCCTGT GCCAGGAGAC CAGCCGCAAC ATGTCAGCGC TCGTCCAGGA ACATGCAGAG 
AGTGTTGCTG CCATACAAAA GCACTACCAG AGCTGCTTAG AGAAAGAGCA TCTTAGCTTC 
ACAGGCACCA TCAGTTCCTT GCAGGAGGAG AATGACATGC TACGAGGGGA GCTATCCTAT 
AAGCTCAGAG AGCTGCAAGA GCAACGGCAG AACCTGTCCC AGCTGGAAGA AGCATTTCAT 
AGGGAGAAAG AAGAGCTGAA GTGCAAACAT GCAGATGAAT TTGGAAGGGC AGAGCAGGAG 
CAGATGACCA GAGAGCTTGA ACTAATGGAC CGTGCTGCCA GTAGCCAACG CAAACTGGAG 
ACCCTGCTGA TAGAAATGGA AGATTCAGAG CTAAAGCATA AGGAACAAGT CCAAAAACTC 
CAGCTTGAAT TTCAGGGTAG GATCCAGGAG CTGGAGCACG CCAATAGAGA GGAGCTCTGC 
AAGCTGAAGA AAAGCTACAG TCAGACCATC TGCTTTCTGG AGAATAAAGG TGAGGCCGAT 
GCGTGTCCAA TGGAGGAAGG CGAAGGATCT GATTCGGTAA CATGCAAGGA GTCCCTGCTC 
CGAGTCCGGG AGCTGGAAAT GCAGTTGAGC AACATGAGAG AGGAGCTAGA ACAGAAACCA 
CTGGATGGAG ATCTGACCAG CCTGAGGGAA AAGTACCAGC GAGACTTGGA CAGTCTCAAG 
GCCACATGCG AGCGTGGATT TGCCGCCATG GAGGAGACGC ATCAGAAAGT GATTGAAGAC 
ATCCAGAGGC AGCATCAGCG GGAGATCCAG AAACTTCTAG AGGAGAAGGA AAGACTGCTG 
GAGGAGGAAA CAAACGCCAC TATTGCTGCA ATTGAGGCCA TGAAAAATGC TCATCGTGAG 
GAACTGGAGA AAACGCAGCG GTCGCAGATG AGCGGTGTGA GCGCAGATAT TCAGGAGCTG 
CACAGACAGT ATGAAGAGGA GTTGCAGTCT ATCCATCGGG AGCTGGAGGT TTTGTCTGAG 
CAGTATTCTC AGAAGTGTCT GGAAAACGCA CATTTGGCTC AAGCGCTGGA AGCTGAGAGA 
CAGGCGCTGG GACAGTGTCA GCGAGAGAAC CAGAAACTGC ATATACACAA CCAGGAACTA 
AACCATCGTC TGAATGAGGA GATCACCAGA ATGCACTCCT GCATCAGCGG AGAAAAAAAC 
ACCTCTTCTC TGACGCAAGG CAAAGACATC TATGAGCTGG AGGTTTTGCT ACGAGTGAAG 
GAGTCCGAGA TCCAGTACCT CAAACAAGAA ATAAACTCTC TGAAGGACGA GCTGCAGTCG 
GCACTTCGAG ATAAGAAGTA TGCTTCAGAT AAATACAAGG ACATTTACAC CGAGCTGAGC 
ATTGTGAAAG CTAAAGCTGA CTGTGACATT AACAAGCTGA GGGAGAAACT TTTGGCTGCT 
ACAGAAGCTC TTGGGGAACT AGACGCTGAG GGAAGCGGTG TTACGGCTGG ATACGACATT 
ATGAAGTCAA AGAGTAACCC AGATTTCCTG AAGAAGGAAA AATCCAGACA GATGCGTGGA 
GTCAGGTCAA AGAGTTTAAA GGAGGGCTTG ACCGTACAGG AGAGGATGAA GCTCTTCGAA 
GCGAAAGACT CCAGGAAGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162958.1
CDS	635..8038
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162901.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162901.4

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GTTCCCTTCC GGATGAACGA 
ACCAGATCAA ATGTGGAGCA GAGCTTCATC CAACCGCCAT CACAGCTCTC CAACAGCTCG 
ATAGTTGAGC CAAAAAGGCC ACATGAGGAC ATCACCGACT CCGCACCTCC TCCAGATCAG 
CGTAAAAGCA GAGGCCACAA GCGTGAGGGC CGGTCCAAAA CCTTCGACTG GTCAGAATTT 
CGACCAGGAC AAGAAAACGA AAGTGGACAA CCCTCACTAA AGAGAGCAGA TACTTCAGTC 
ATTATCTCAT CTTCTCCATC ATCCATCGCT TCCTCTCCCA TCTCTAGCTC TTCTTCTCAT 
CAGACGTCTT CCTCCATCAC GATCATCAGC CATACTGAAA AGTTGCCACA GCATAATGAA 
GAAGAAGAAC AAGTGGTGCA GGAGTATGTA CCGCACCATC AGACTGTACC TACAACAATG 
ACCGGTCCAG AGACCCCGTC AAACCCTCAT AATGTGCCAC AGGAACCGGC AGGAATAGAT 
TTGAACCAGT CCAGAGAAGT GCAGGAGGTC GATGGTCATG GAGTGATCCG CAAAAACTCT 
GATGTCCAAG TGGAAATTGA ACAGAGGTGG CATCAGGTGG AAACGACGCC CCTCAGGGAG 
GAAAAGCAGG TACCGATCGC TGGTGGCTCA CCTAACGTAT CAAATGTGTC TGTTGGAGAA 
AGAGCCTCTC CGCAAGAGAA GACCAGCAAA TTGGAAAAAG AGTTGGGTCA GGCACAAAAG 
GAGTTGGCCA GACTCCAGGA GCAGAACAGC ATCTTACAAG ACCAACTTCA GGATGCACGC 
GGGAGAGAGC AGAATGCACG GGAAGGATAT GTACTTCAGA ATGACTCGAC TATAGCAAAG 
CCTTCATCTG ATGCTCCCGT GTCTACCACG GTTCATAAAG CACCATGGCA GCGCCTAAGC 
AAACTAAACC AGGATCTAAA GTCTGAGCTG GAGTCTCAGC GGCGGAAGCA AGATAAGGCC 
AAACATCAAG TTAGCAGCTT GCGTCGTAGC TACAGTGAAG CAAAAGATAT AATAGGGCAC 
CATGAGGCTG AAATCGAGGC CTTGGAAGAA AAGCTAACGG CTGCCATGGC AGAGATTGTA 
GTGAGTGAGC AGGCTGTAGC ACGAATGCGC AGTGATCTGA AAATTGAACG CGTTCGTTGT 
CAAGAGCGTG AGGAAGAATG GACACGAAAT GAGACAACAT TACGGTCCCA GCTACAAGAT 
AGTGAAGAAC GTCTTCGGCA AGTGGAGGCC AGTTTGCTTG AGAAGAATCA AGAGCTGAGG 
CAGCTGGAGC AGCAGCAAGC TTTACAAAGG GACCAGCACA GAGAGATGCA GAGGTTACAG 
GAGAGACTCG CTGAGGCTAC AGGGCGTCTG ATTTCCATGG AGGAGTCTCA GACTATGAGG 
GAGGAAAGGG AGAGAAAAGA GCACCGGAGT CTGGAGGAGA AACATGAGCG GGAACGACAA 
GGGCTGACTC GAAGGTTGGC AGAATCTGAA GAAAGGAGAC AAGAAATAGA AGAGCAATTG 
CAGGAGGCTC AAGAACAGGT GGAGGCACTG CTGAGGGGCA GCAGTGGGAT GGAAACTGTG 
GGATTGAGGG AGCAAGTGCA TCGTCTTCAG CAGGAGTTGG AGACACAGAC TGATATGGTG 
GAAATGCTGC GAGAAAGTGT GAGAAGGCTG GAGGAGGAAC GTGACCAGCT TACTTGCCGC 
TGTCAGGAAC TCCTCAATCA GATTGCAGAG GCTGACAGAG AGGTTGGAAA GCAACAGGCA 
TGTTTAACAA CGGTGGAAAC AGACTTTCAC TCCCTTGAGA CCTCATATGA GCATGTATCT 
GAGGAATTTG CTAGGATAAG TCATGTGCTA CAAGAGAAAG AAGAGGAAGT GAGGCAGACA 
AAGGAAATGT ATGAGCATCT TATCAGACAA AAGGAGCAGG ACTTGAGTGA AGCCCTCATC 
AAGATGGCTG CCTTAGGCAG TAGTTTAGAA GAAACAGAAC TTTGCTTGCA GCAAAAAGAA 
GAACTTCTTT CTAAAATGGA GCGTGACTAT CCAGGACTGG TAGAGTCCCG AAAAGCAGAA 
CAGGAGCTGC AGGCAAAACT GGTAGTTGCA GAGGATCGAA TCGCTGAGCT GGAGGAACAC 
CTCAATGCCC TGCGACTGGG ATATGCAGAT CTCAGAATGC GACGTTGTCG CTCTCAGGAA 
GACATGCTTG AGAGTTTAAA GGAGATTGAA CATGAAGTTT CCCTATCCTC AAGTACATCC 
CAGCTTCTTG CTAGAAGCAG TTCAGAGACT GAAATGTCAT TAGTAAAGCG ACAGAGGATT 
CGCTTTTCCA CTATCCAGTG CCAAACCTAC CACCGGTCCC AGGGAGCAGA CAAGTTTCAG 
ACAGAAAACA CATCTTTGGA TCTTACACAA GATCTGAGTC TAGACCACAT GCATGACCTG 
AGCCTGACTC AAGATATTAG TGTAGTCAGT GACAATTCAT TTCAGCAATG CAGAGATCCT 
GAAAAGTTCA TCTCCATCAT ACATGCTCTT GAAACCAAGC TGTTAGCCAC TGAGGAGAAA 
CTCCGGCACC TTACGGATAA GATACGGGAC CAGCCCAATC TTTCAGCGGT GCAGGAACAT 
CTCAGTACTA GAGCTACCAT GGAGGACTGG ACAACTAAAA CCTGTGATGA CTTGCCAATG 
GATGAACCCA GCCAGAAGCA CCTAAACAAG ACTGTCAGTG AACATGACAG TGGTGAATAT 
GAGAAGGCAT TGGCACTGGT AGAGTCTTGC ACTGAAAGAG TCAGAGAGAT TCTCAGTAAT 
CAGAATGAGA CCAGTTCTGC TGAATCTCTG ATTTGTACAC TTGCAGAAGT AGAGAAAAGG 
CTGGTTTGTG CAACTGCATT CCTAAGGAAA GGCCACACAT TATATGAGTC GTGCCACGTA 
TTTGATGCAC CAGATGTGCA GTCAATGAAG GGAAGTATTA AGCTGTTTGC CAGAACATTG 
TGTTTTGAGG CTGAAGTTCT GGAGAAGATG GGGTTCTCTT TGCAGGACTT GAACTCTGAT 
ATTATGGCAG CCCTGAACTC CATTCACAAG GAGGCTGAAA ACATCAAGAA GAGCTGTGAT 
GGCTGTCTCT CAGTTGTCTA TGCTGATTTG CTTACAAGGA AACTTCTGCT AGAAACCATG 
TTCATGTCAG AGATAGACAA ACTTGAGACA GGAAATCTAG CGAATAGTGG TTTTTCACAG 
GATGCCATTA AGAGTGCATG CATTAGTGCT GAGCTTGCAT ATTCACTTCA GAACCTAACA 
ACGAATTTCC AGGAAAGATT TGATGAGCTT CAAAAGGACT TGATTCAGGC TAACAAGACC 
TTGAAACAAA GGGACTTTGC TCTGAAAGAT GCTGTTAGTG CATCAAAGCA TTCATGCATT 
GAAAGCATCC CCCAAAGTAC GACCGATCAT CTTGTTGGTG ATGATGCCCC TCCTGAACTT 
GTTCCCTATA TGGAGCAGAT TGAGATGGAG GAAGCTCAGA GTTTAGCTGC GGAGATTGTT 
CGTAGACATT TAACAGGTGG CATGCTATCC CACAGTGCTG AATCAGAATC ACATCTCCAA 
ATGGGCTGGG AAAATCTTAT TGCTGAGCTG AAGAAGCAAG CCAAAGCTCT TCGCAATATT 
TCTCAGGAAA TTGAGAAGAT CTGCGAGACG GGAGAAATGA ATTCCCTCTC CAGACTTGCT 
GAGGCAATCC AATTAAGCTC ATACCGCAGC GACTCCAGCT CTGTTTGTAT GCGAGAGGCT 
TTAATGCAAG TTCAGGTCGC CTATGTTTCT TGCAGGTTGA GAGCGGGCCA CACAAGGGAA 
CTTTCCCTGT GCCAGGAGAC CAGCCGCAAC ATGTCAGCGC TCGTCCAGGA ACATGCAGAG 
AGTGTTGCTG CCATACAAAA GCACTACCAG AGCTGCTTAG AGAAAGAGCA TCTTAGCTTC 
ACAGGCACCA TCAGTTCCTT GCAGGAGGAG AATGACATGC TACGAGGGGA GCTATCCTAT 
AAGCTCAGAG AGCTGCAAGA GCAACGGCAG AACCTGTCCC AGCTGGAAGA AGCATTTCAT 
AGGGAGAAAG AAGAGCTGAA GTGCAAACAT GCAGATGAAT TTGGAAGGGC AGAGCAGGAG 
CAGATGACCA GAGAGCTTGA ACTAATGGAC CGTGCTGCCA GTAGCCAACG CAAACTGGAG 
ACCCTGCTGA TAGAAATGGA AGATTCAGAG CTAAAGCATA AGGAACAAGT CCAAAAACTC 
CAGCTTGAAT TTCAGGGTAG GATCCAGGAG CTGGAGCACG CCAATAGAGA GGAGCTCTGC 
AAGCTGAAGA AAAGCTACAG TCAGACCATC TGCTTTCTGG AGAATAAAGG TGAGGCCGAT 
GCGTGTCCAA TGGAGGAAGG CGAAGGATCT GATTCGGTAA CATGCAAGGA GTCCCTGCTC 
CGAGTCCGGG AGCTGGAAAT GCAGTTGAGC AACATGAGAG AGGAGCTAGA ACAGAAACCA 
CTGGATGGAG ATCTGACCAG CCTGAGGGAA AAGTACCAGC GAGACTTGGA CAGTCTCAAG 
GCCACATGCG AGCGTGGATT TGCCGCCATG GAGGAGACGC ATCAGAAAGT GATTGAAGAC 
ATCCAGAGGC AGCATCAGCG GGAGATCCAG AAACTTCTAG AGGAGAAGGA AAGACTGCTG 
GAGGAGGAAA CAAACGCCAC TATTGCTGCA ATTGAGGCCA TGAAAAATGC TCATCGTGAG 
GAACTGGAGA AAACGCAGCG GTCGCAGATG AGCGGTGTGA GCGCAGATAT TCAGGAGCTG 
CACAGACAGT ATGAAGAGGA GTTGCAGTCT ATCCATCGGG AGCTGGAGGT TTTGTCTGAG 
CAGTATTCTC AGAAGTGTCT GGAAAACGCA CATTTGGCTC AAGCGCTGGA AGCTGAGAGA 
CAGGCGCTGG GACAGTGTCA GCGAGAGAAC CAGAAACTGC ATATACACAA CCAGGAACTA 
AACCATCGTC TGAATGAGGA GATCACCAGA ATGCACTCCT GCATCAGCGG AGAAAAAAAC 
ACCTCTTCTC TGACGCAAGG CAAAGACATC TATGAGCTGG AGGTTTTGCT ACGAGTGAAG 
GAGTCCGAGA TCCAGTACCT CAAACAAGAA ATAAACTCTC TGAAGGACGA GCTGCAGTCG 
GCACTTCGAG ATAAGAAGTA TGCTTCAGAT AAATACAAGG ACATTTACAC CGAGCTGAGC 
ATTGTGAAAG CTAAAGCTGA CTGTGACATT AACAAGCTGA GGGAGAAACT TTTGGCTGCT 
ACAGAAGCTC TTGGGGAACT AGACGCTGAG GGAAGCGGTG TTACGGCTGG ATACGACATT 
ATGAAGTCAA AGAGTAACCC AGATTTCCTG AAGAAGGAAA AATCCAGACA GATGCGTGGA 
GTCAGGTCAA AGAGTTTAAA GGAGGGCTTG ACCGTACAGG AGAGGATGAA GCTCTTCGAA 
GCGAAAGACT CCAGGAAGAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162902.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162959.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X3, mRNA.

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CloneID	ODa28903
Clone ID Related Accession (Same CDS sequence)	XM_005162902.3 , XM_009297658.2 , XM_009297658.3 , XM_005162902.4
Accession Version	XM_005162902.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centrosome-associated protein CEP250 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GGCAGACAGT 
GAGGGGTTAA TCGAGGGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162959.1
CDS	627..8030
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKADSEGLIEGF

Target ORF information:

RefSeq Version	XM_005162902.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162902.3

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GGCAGACAGT 
GAGGGGTTAA TCGAGGGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_009297658.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295933.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X4, mRNA.

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CloneID	ODa28903
Clone ID Related Accession (Same CDS sequence)	XM_005162902.3 , XM_009297658.2 , XM_009297658.3 , XM_005162902.4
Accession Version	XM_009297658.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centrosome-associated protein CEP250 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GGCAGACAGT 
GAGGGGTTAA TCGAGGGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295933.1
CDS	627..8030
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKADSEGLIEGF

Target ORF information:

RefSeq Version	XM_009297658.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297658.2

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GGCAGACAGT 
GAGGGGTTAA TCGAGGGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_009297658.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295933.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X4, mRNA.

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CloneID	ODa28903
Clone ID Related Accession (Same CDS sequence)	XM_005162902.3 , XM_009297658.2 , XM_009297658.3 , XM_005162902.4
Accession Version	XM_009297658.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297658.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GGCAGACAGT 
GAGGGGTTAA TCGAGGGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295933.1
CDS	635..8038
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKADSEGLIEGF

Target ORF information:

RefSeq Version	XM_009297658.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297658.3

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GGCAGACAGT 
GAGGGGTTAA TCGAGGGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162902.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162959.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X3, mRNA.

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CloneID	ODa28903
Clone ID Related Accession (Same CDS sequence)	XM_005162902.3 , XM_009297658.2 , XM_009297658.3 , XM_005162902.4
Accession Version	XM_005162902.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162902.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GGCAGACAGT 
GAGGGGTTAA TCGAGGGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162959.1
CDS	635..8038
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRYLSFDPELYSSPISSHKHTSHDPHSTVHTQASSVVCDAHKHNMQSKSEKHLSQSSEILSDSTLNRDSNIYSPNPHANVLPSSLSSSQSSLDSEQSPESVVGGALGRAGRSGCGYVALADVPRARRLSHRDAYRCSQKRQELRARTRSPGREEVERLFGCQRRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKADSEGLIEGF

Target ORF information:

RefSeq Version	XM_005162902.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162902.4

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGTATCTCTC CTTTGATCCA 
GAGCTCTACA GTTCTCCAAT CAGCTCACAC AAACACACGT CACATGACCC ACACAGCACT 
GTGCACACGC AGGCTTCCAG TGTTGTGTGT GACGCTCATA AACACAACAT GCAAAGCAAA 
TCAGAGAAGC ATCTTTCTCA GTCGTCGGAG ATCCTGTCAG ATTCAACGCT GAACCGGGAT 
TCAAACATCT ACAGTCCGAA CCCGCACGCA AACGTCCTCC CATCATCCCT GTCCTCCTCT 
CAGAGCTCTT TGGACTCTGA GCAGAGTCCG GAGAGTGTAG TGGGTGGAGC CTTGGGCAGG 
GCGGGGCGCA GTGGGTGTGG TTATGTGGCT CTGGCTGATG TGCCAAGGGC ACGGAGACTC 
AGCCATCGGG ATGCGTATCG ATGCAGTCAG AAGCGGCAGG AGCTGCGGGC GCGCACCAGG 
AGCCCTGGCA GAGAGGAGGT GGAGCGGCTG TTCGGATGCC AGCGAAGGCG TTCGCAGGTT 
ATTGAGAGAT TTGAGGGTCA GGACACTGTC AACGCTCCAG AGCAAATGGA CACCACGAGT 
TCCTCAACAG ATCCCGCCCC TTCTTACTCT AAAACAGCCA ATCAGAGAGC AGGACGCAAT 
GAGAAACGCC TCTCCAACCA GAAACAGGAG TTTTCCTTGG ATTCAGCTAA ATGCCGGACT 
GATGAGGTTT CTTCATTAGC TGGTTACCGG AGGGCCAAAA GTCTGGATCG CAGAACCACA 
GAAACACTCA TGACTCCTGA CCTGCTGAAC TTTAAGAAAG GATGGATGAC CAAACTATAC 
GAAGATGGAT TGTGGAAGAA ACACTGGTTT GTTCTGACCG ATCAGAGTCT GAGGTTCTAC 
CGTGACTCCA TTGCTGAGGA GGCTTCTGAT CTGGATGGAG AGATCGATCT GTCCTTGTGT 
TATGATGTGA CCGAATTCCC AGTTCAGAGG AACTACGGCT TCCAAATCCT CAGCAAAGAA 
GGGGCGTTCA CACTGTCTGC TATGACTTCT GGGATACGTC GTAACTGGAT TCAAGCCATT 
CTGAAAAACA TGCGGCCCAC TGTCGCTCCT GATGTCACAC GGAAAAACGT CTCTCTGAAA 
CTTTCAGTTC TTATGCCCAG TTCCCTTCCG GATGAACGAA CCAGATCAAA TGTGGAGCAG 
AGCTTCATCC AACCGCCATC ACAGCTCTCC AACAGCTCGA TAGTTGAGCC AAAAAGGCCA 
CATGAGGACA TCACCGACTC CGCACCTCCT CCAGATCAGC GTAAAAGCAG AGGCCACAAG 
CGTGAGGGCC GGTCCAAAAC CTTCGACTGG TCAGAATTTC GACCAGGACA AGAAAACGAA 
AGTGGACAAC CCTCACTAAA GAGAGCAGAT ACTTCAGTCA TTATCTCATC TTCTCCATCA 
TCCATCGCTT CCTCTCCCAT CTCTAGCTCT TCTTCTCATC AGACGTCTTC CTCCATCACG 
ATCATCAGCC ATACTGAAAA GTTGCCACAG CATAATGAAG AAGAAGAACA AGTGGTGCAG 
GAGTATGTAC CGCACCATCA GACTGTACCT ACAACAATGA CCGGTCCAGA GACCCCGTCA 
AACCCTCATA ATGTGCCACA GGAACCGGCA GGAATAGATT TGAACCAGTC CAGAGAAGTG 
CAGGAGGTCG ATGGTCATGG AGTGATCCGC AAAAACTCTG ATGTCCAAGT GGAAATTGAA 
CAGAGGTGGC ATCAGGTGGA AACGACGCCC CTCAGGGAGG AAAAGCAGGT ACCGATCGCT 
GGTGGCTCAC CTAACGTATC AAATGTGTCT GTTGGAGAAA GAGCCTCTCC GCAAGAGAAG 
ACCAGCAAAT TGGAAAAAGA GTTGGGTCAG GCACAAAAGG AGTTGGCCAG ACTCCAGGAG 
CAGAACAGCA TCTTACAAGA CCAACTTCAG GATGCACGCG GGAGAGAGCA GAATGCACGG 
GAAGGATATG TACTTCAGAA TGACTCGACT ATAGCAAAGC CTTCATCTGA TGCTCCCGTG 
TCTACCACGG TTCATAAAGC ACCATGGCAG CGCCTAAGCA AACTAAACCA GGATCTAAAG 
TCTGAGCTGG AGTCTCAGCG GCGGAAGCAA GATAAGGCCA AACATCAAGT TAGCAGCTTG 
CGTCGTAGCT ACAGTGAAGC AAAAGATATA ATAGGGCACC ATGAGGCTGA AATCGAGGCC 
TTGGAAGAAA AGCTAACGGC TGCCATGGCA GAGATTGTAG TGAGTGAGCA GGCTGTAGCA 
CGAATGCGCA GTGATCTGAA AATTGAACGC GTTCGTTGTC AAGAGCGTGA GGAAGAATGG 
ACACGAAATG AGACAACATT ACGGTCCCAG CTACAAGATA GTGAAGAACG TCTTCGGCAA 
GTGGAGGCCA GTTTGCTTGA GAAGAATCAA GAGCTGAGGC AGCTGGAGCA GCAGCAAGCT 
TTACAAAGGG ACCAGCACAG AGAGATGCAG AGGTTACAGG AGAGACTCGC TGAGGCTACA 
GGGCGTCTGA TTTCCATGGA GGAGTCTCAG ACTATGAGGG AGGAAAGGGA GAGAAAAGAG 
CACCGGAGTC TGGAGGAGAA ACATGAGCGG GAACGACAAG GGCTGACTCG AAGGTTGGCA 
GAATCTGAAG AAAGGAGACA AGAAATAGAA GAGCAATTGC AGGAGGCTCA AGAACAGGTG 
GAGGCACTGC TGAGGGGCAG CAGTGGGATG GAAACTGTGG GATTGAGGGA GCAAGTGCAT 
CGTCTTCAGC AGGAGTTGGA GACACAGACT GATATGGTGG AAATGCTGCG AGAAAGTGTG 
AGAAGGCTGG AGGAGGAACG TGACCAGCTT ACTTGCCGCT GTCAGGAACT CCTCAATCAG 
ATTGCAGAGG CTGACAGAGA GGTTGGAAAG CAACAGGCAT GTTTAACAAC GGTGGAAACA 
GACTTTCACT CCCTTGAGAC CTCATATGAG CATGTATCTG AGGAATTTGC TAGGATAAGT 
CATGTGCTAC AAGAGAAAGA AGAGGAAGTG AGGCAGACAA AGGAAATGTA TGAGCATCTT 
ATCAGACAAA AGGAGCAGGA CTTGAGTGAA GCCCTCATCA AGATGGCTGC CTTAGGCAGT 
AGTTTAGAAG AAACAGAACT TTGCTTGCAG CAAAAAGAAG AACTTCTTTC TAAAATGGAG 
CGTGACTATC CAGGACTGGT AGAGTCCCGA AAAGCAGAAC AGGAGCTGCA GGCAAAACTG 
GTAGTTGCAG AGGATCGAAT CGCTGAGCTG GAGGAACACC TCAATGCCCT GCGACTGGGA 
TATGCAGATC TCAGAATGCG ACGTTGTCGC TCTCAGGAAG ACATGCTTGA GAGTTTAAAG 
GAGATTGAAC ATGAAGTTTC CCTATCCTCA AGTACATCCC AGCTTCTTGC TAGAAGCAGT 
TCAGAGACTG AAATGTCATT AGTAAAGCGA CAGAGGATTC GCTTTTCCAC TATCCAGTGC 
CAAACCTACC ACCGGTCCCA GGGAGCAGAC AAGTTTCAGA CAGAAAACAC ATCTTTGGAT 
CTTACACAAG ATCTGAGTCT AGACCACATG CATGACCTGA GCCTGACTCA AGATATTAGT 
GTAGTCAGTG ACAATTCATT TCAGCAATGC AGAGATCCTG AAAAGTTCAT CTCCATCATA 
CATGCTCTTG AAACCAAGCT GTTAGCCACT GAGGAGAAAC TCCGGCACCT TACGGATAAG 
ATACGGGACC AGCCCAATCT TTCAGCGGTG CAGGAACATC TCAGTACTAG AGCTACCATG 
GAGGACTGGA CAACTAAAAC CTGTGATGAC TTGCCAATGG ATGAACCCAG CCAGAAGCAC 
CTAAACAAGA CTGTCAGTGA ACATGACAGT GGTGAATATG AGAAGGCATT GGCACTGGTA 
GAGTCTTGCA CTGAAAGAGT CAGAGAGATT CTCAGTAATC AGAATGAGAC CAGTTCTGCT 
GAATCTCTGA TTTGTACACT TGCAGAAGTA GAGAAAAGGC TGGTTTGTGC AACTGCATTC 
CTAAGGAAAG GCCACACATT ATATGAGTCG TGCCACGTAT TTGATGCACC AGATGTGCAG 
TCAATGAAGG GAAGTATTAA GCTGTTTGCC AGAACATTGT GTTTTGAGGC TGAAGTTCTG 
GAGAAGATGG GGTTCTCTTT GCAGGACTTG AACTCTGATA TTATGGCAGC CCTGAACTCC 
ATTCACAAGG AGGCTGAAAA CATCAAGAAG AGCTGTGATG GCTGTCTCTC AGTTGTCTAT 
GCTGATTTGC TTACAAGGAA ACTTCTGCTA GAAACCATGT TCATGTCAGA GATAGACAAA 
CTTGAGACAG GAAATCTAGC GAATAGTGGT TTTTCACAGG ATGCCATTAA GAGTGCATGC 
ATTAGTGCTG AGCTTGCATA TTCACTTCAG AACCTAACAA CGAATTTCCA GGAAAGATTT 
GATGAGCTTC AAAAGGACTT GATTCAGGCT AACAAGACCT TGAAACAAAG GGACTTTGCT 
CTGAAAGATG CTGTTAGTGC ATCAAAGCAT TCATGCATTG AAAGCATCCC CCAAAGTACG 
ACCGATCATC TTGTTGGTGA TGATGCCCCT CCTGAACTTG TTCCCTATAT GGAGCAGATT 
GAGATGGAGG AAGCTCAGAG TTTAGCTGCG GAGATTGTTC GTAGACATTT AACAGGTGGC 
ATGCTATCCC ACAGTGCTGA ATCAGAATCA CATCTCCAAA TGGGCTGGGA AAATCTTATT 
GCTGAGCTGA AGAAGCAAGC CAAAGCTCTT CGCAATATTT CTCAGGAAAT TGAGAAGATC 
TGCGAGACGG GAGAAATGAA TTCCCTCTCC AGACTTGCTG AGGCAATCCA ATTAAGCTCA 
TACCGCAGCG ACTCCAGCTC TGTTTGTATG CGAGAGGCTT TAATGCAAGT TCAGGTCGCC 
TATGTTTCTT GCAGGTTGAG AGCGGGCCAC ACAAGGGAAC TTTCCCTGTG CCAGGAGACC 
AGCCGCAACA TGTCAGCGCT CGTCCAGGAA CATGCAGAGA GTGTTGCTGC CATACAAAAG 
CACTACCAGA GCTGCTTAGA GAAAGAGCAT CTTAGCTTCA CAGGCACCAT CAGTTCCTTG 
CAGGAGGAGA ATGACATGCT ACGAGGGGAG CTATCCTATA AGCTCAGAGA GCTGCAAGAG 
CAACGGCAGA ACCTGTCCCA GCTGGAAGAA GCATTTCATA GGGAGAAAGA AGAGCTGAAG 
TGCAAACATG CAGATGAATT TGGAAGGGCA GAGCAGGAGC AGATGACCAG AGAGCTTGAA 
CTAATGGACC GTGCTGCCAG TAGCCAACGC AAACTGGAGA CCCTGCTGAT AGAAATGGAA 
GATTCAGAGC TAAAGCATAA GGAACAAGTC CAAAAACTCC AGCTTGAATT TCAGGGTAGG 
ATCCAGGAGC TGGAGCACGC CAATAGAGAG GAGCTCTGCA AGCTGAAGAA AAGCTACAGT 
CAGACCATCT GCTTTCTGGA GAATAAAGGT GAGGCCGATG CGTGTCCAAT GGAGGAAGGC 
GAAGGATCTG ATTCGGTAAC ATGCAAGGAG TCCCTGCTCC GAGTCCGGGA GCTGGAAATG 
CAGTTGAGCA ACATGAGAGA GGAGCTAGAA CAGAAACCAC TGGATGGAGA TCTGACCAGC 
CTGAGGGAAA AGTACCAGCG AGACTTGGAC AGTCTCAAGG CCACATGCGA GCGTGGATTT 
GCCGCCATGG AGGAGACGCA TCAGAAAGTG ATTGAAGACA TCCAGAGGCA GCATCAGCGG 
GAGATCCAGA AACTTCTAGA GGAGAAGGAA AGACTGCTGG AGGAGGAAAC AAACGCCACT 
ATTGCTGCAA TTGAGGCCAT GAAAAATGCT CATCGTGAGG AACTGGAGAA AACGCAGCGG 
TCGCAGATGA GCGGTGTGAG CGCAGATATT CAGGAGCTGC ACAGACAGTA TGAAGAGGAG 
TTGCAGTCTA TCCATCGGGA GCTGGAGGTT TTGTCTGAGC AGTATTCTCA GAAGTGTCTG 
GAAAACGCAC ATTTGGCTCA AGCGCTGGAA GCTGAGAGAC AGGCGCTGGG ACAGTGTCAG 
CGAGAGAACC AGAAACTGCA TATACACAAC CAGGAACTAA ACCATCGTCT GAATGAGGAG 
ATCACCAGAA TGCACTCCTG CATCAGCGGA GAAAAAAACA CCTCTTCTCT GACGCAAGGC 
AAAGACATCT ATGAGCTGGA GGTTTTGCTA CGAGTGAAGG AGTCCGAGAT CCAGTACCTC 
AAACAAGAAA TAAACTCTCT GAAGGACGAG CTGCAGTCGG CACTTCGAGA TAAGAAGTAT 
GCTTCAGATA AATACAAGGA CATTTACACC GAGCTGAGCA TTGTGAAAGC TAAAGCTGAC 
TGTGACATTA ACAAGCTGAG GGAGAAACTT TTGGCTGCTA CAGAAGCTCT TGGGGAACTA 
GACGCTGAGG GAAGCGGTGT TACGGCTGGA TACGACATTA TGAAGTCAAA GAGTAACCCA 
GATTTCCTGA AGAAGGAAAA ATCCAGACAG ATGCGTGGAG TCAGGTCAAA GGCAGACAGT 
GAGGGGTTAA TCGAGGGTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162903.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162960.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X5, mRNA.

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CloneID	ODa28904
Clone ID Related Accession (Same CDS sequence)	XM_005162903.3 , XM_005162903.4
Accession Version	XM_005162903.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	centrosome-associated protein CEP250 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162903.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGCGTTCGCA GGTTATTGAG 
AGATTTGAGG GTCAGGACAC TGTCAACGCT CCAGAGCAAA TGGACACCAC GAGTTCCTCA 
ACAGATCCCG CCCCTTCTTA CTCTAAAACA GCCAATCAGA GAGCAGGACG CAATGAGAAA 
CGCCTCTCCA ACCAGAAACA GGAGTTTTCC TTGGATTCAG CTAAATGCCG GACTGATGAG 
GTTTCTTCAT TAGCTGGTTA CCGGAGGGCC AAAAGTCTGG ATCGCAGAAC CACAGAAACA 
CTCATGACTC CTGACCTGCT GAACTTTAAG AAAGGATGGA TGACCAAACT ATACGAAGAT 
GGATTGTGGA AGAAACACTG GTTTGTTCTG ACCGATCAGA GTCTGAGGTT CTACCGTGAC 
TCCATTGCTG AGGAGGCTTC TGATCTGGAT GGAGAGATCG ATCTGTCCTT GTGTTATGAT 
GTGACCGAAT TCCCAGTTCA GAGGAACTAC GGCTTCCAAA TCCTCAGCAA AGAAGGGGCG 
TTCACACTGT CTGCTATGAC TTCTGGGATA CGTCGTAACT GGATTCAAGC CATTCTGAAA 
AACATGCGGC CCACTGTCGC TCCTGATGTC ACACGGAAAA ACGTCTCTCT GAAACTTTCA 
GTTCTTATGC CCAGTTCCCT TCCGGATGAA CGAACCAGAT CAAATGTGGA GCAGAGCTTC 
ATCCAACCGC CATCACAGCT CTCCAACAGC TCGATAGTTG AGCCAAAAAG GCCACATGAG 
GACATCACCG ACTCCGCACC TCCTCCAGAT CAGCGTAAAA GCAGAGGCCA CAAGCGTGAG 
GGCCGGTCCA AAACCTTCGA CTGGTCAGAA TTTCGACCAG GACAAGAAAA CGAAAGTGGA 
CAACCCTCAC TAAAGAGAGC AGATACTTCA GTCATTATCT CATCTTCTCC ATCATCCATC 
GCTTCCTCTC CCATCTCTAG CTCTTCTTCT CATCAGACGT CTTCCTCCAT CACGATCATC 
AGCCATACTG AAAAGTTGCC ACAGCATAAT GAAGAAGAAG AACAAGTGGT GCAGGAGTAT 
GTACCGCACC ATCAGACTGT ACCTACAACA ATGACCGGTC CAGAGACCCC GTCAAACCCT 
CATAATGTGC CACAGGAACC GGCAGGAATA GATTTGAACC AGTCCAGAGA AGTGCAGGAG 
GTCGATGGTC ATGGAGTGAT CCGCAAAAAC TCTGATGTCC AAGTGGAAAT TGAACAGAGG 
TGGCATCAGG TGGAAACGAC GCCCCTCAGG GAGGAAAAGC AGGTACCGAT CGCTGGTGGC 
TCACCTAACG TATCAAATGT GTCTGTTGGA GAAAGAGCCT CTCCGCAAGA GAAGACCAGC 
AAATTGGAAA AAGAGTTGGG TCAGGCACAA AAGGAGTTGG CCAGACTCCA GGAGCAGAAC 
AGCATCTTAC AAGACCAACT TCAGGATGCA CGCGGGAGAG AGCAGAATGC ACGGGAAGGA 
TATGTACTTC AGAATGACTC GACTATAGCA AAGCCTTCAT CTGATGCTCC CGTGTCTACC 
ACGGTTCATA AAGCACCATG GCAGCGCCTA AGCAAACTAA ACCAGGATCT AAAGTCTGAG 
CTGGAGTCTC AGCGGCGGAA GCAAGATAAG GCCAAACATC AAGTTAGCAG CTTGCGTCGT 
AGCTACAGTG AAGCAAAAGA TATAATAGGG CACCATGAGG CTGAAATCGA GGCCTTGGAA 
GAAAAGCTAA CGGCTGCCAT GGCAGAGATT GTAGTGAGTG AGCAGGCTGT AGCACGAATG 
CGCAGTGATC TGAAAATTGA ACGCGTTCGT TGTCAAGAGC GTGAGGAAGA ATGGACACGA 
AATGAGACAA CATTACGGTC CCAGCTACAA GATAGTGAAG AACGTCTTCG GCAAGTGGAG 
GCCAGTTTGC TTGAGAAGAA TCAAGAGCTG AGGCAGCTGG AGCAGCAGCA AGCTTTACAA 
AGGGACCAGC ACAGAGAGAT GCAGAGGTTA CAGGAGAGAC TCGCTGAGGC TACAGGGCGT 
CTGATTTCCA TGGAGGAGTC TCAGACTATG AGGGAGGAAA GGGAGAGAAA AGAGCACCGG 
AGTCTGGAGG AGAAACATGA GCGGGAACGA CAAGGGCTGA CTCGAAGGTT GGCAGAATCT 
GAAGAAAGGA GACAAGAAAT AGAAGAGCAA TTGCAGGAGG CTCAAGAACA GGTGGAGGCA 
CTGCTGAGGG GCAGCAGTGG GATGGAAACT GTGGGATTGA GGGAGCAAGT GCATCGTCTT 
CAGCAGGAGT TGGAGACACA GACTGATATG GTGGAAATGC TGCGAGAAAG TGTGAGAAGG 
CTGGAGGAGG AACGTGACCA GCTTACTTGC CGCTGTCAGG AACTCCTCAA TCAGATTGCA 
GAGGCTGACA GAGAGGTTGG AAAGCAACAG GCATGTTTAA CAACGGTGGA AACAGACTTT 
CACTCCCTTG AGACCTCATA TGAGCATGTA TCTGAGGAAT TTGCTAGGAT AAGTCATGTG 
CTACAAGAGA AAGAAGAGGA AGTGAGGCAG ACAAAGGAAA TGTATGAGCA TCTTATCAGA 
CAAAAGGAGC AGGACTTGAG TGAAGCCCTC ATCAAGATGG CTGCCTTAGG CAGTAGTTTA 
GAAGAAACAG AACTTTGCTT GCAGCAAAAA GAAGAACTTC TTTCTAAAAT GGAGCGTGAC 
TATCCAGGAC TGGTAGAGTC CCGAAAAGCA GAACAGGAGC TGCAGGCAAA ACTGGTAGTT 
GCAGAGGATC GAATCGCTGA GCTGGAGGAA CACCTCAATG CCCTGCGACT GGGATATGCA 
GATCTCAGAA TGCGACGTTG TCGCTCTCAG GAAGACATGC TTGAGAGTTT AAAGGAGATT 
GAACATGAAG TTTCCCTATC CTCAAGTACA TCCCAGCTTC TTGCTAGAAG CAGTTCAGAG 
ACTGAAATGT CATTAGTAAA GCGACAGAGG ATTCGCTTTT CCACTATCCA GTGCCAAACC 
TACCACCGGT CCCAGGGAGC AGACAAGTTT CAGACAGAAA ACACATCTTT GGATCTTACA 
CAAGATCTGA GTCTAGACCA CATGCATGAC CTGAGCCTGA CTCAAGATAT TAGTGTAGTC 
AGTGACAATT CATTTCAGCA ATGCAGAGAT CCTGAAAAGT TCATCTCCAT CATACATGCT 
CTTGAAACCA AGCTGTTAGC CACTGAGGAG AAACTCCGGC ACCTTACGGA TAAGATACGG 
GACCAGCCCA ATCTTTCAGC GGTGCAGGAA CATCTCAGTA CTAGAGCTAC CATGGAGGAC 
TGGACAACTA AAACCTGTGA TGACTTGCCA ATGGATGAAC CCAGCCAGAA GCACCTAAAC 
AAGACTGTCA GTGAACATGA CAGTGGTGAA TATGAGAAGG CATTGGCACT GGTAGAGTCT 
TGCACTGAAA GAGTCAGAGA GATTCTCAGT AATCAGAATG AGACCAGTTC TGCTGAATCT 
CTGATTTGTA CACTTGCAGA AGTAGAGAAA AGGCTGGTTT GTGCAACTGC ATTCCTAAGG 
AAAGGCCACA CATTATATGA GTCGTGCCAC GTATTTGATG CACCAGATGT GCAGTCAATG 
AAGGGAAGTA TTAAGCTGTT TGCCAGAACA TTGTGTTTTG AGGCTGAAGT TCTGGAGAAG 
ATGGGGTTCT CTTTGCAGGA CTTGAACTCT GATATTATGG CAGCCCTGAA CTCCATTCAC 
AAGGAGGCTG AAAACATCAA GAAGAGCTGT GATGGCTGTC TCTCAGTTGT CTATGCTGAT 
TTGCTTACAA GGAAACTTCT GCTAGAAACC ATGTTCATGT CAGAGATAGA CAAACTTGAG 
ACAGGAAATC TAGCGAATAG TGGTTTTTCA CAGGATGCCA TTAAGAGTGC ATGCATTAGT 
GCTGAGCTTG CATATTCACT TCAGAACCTA ACAACGAATT TCCAGGAAAG ATTTGATGAG 
CTTCAAAAGG ACTTGATTCA GGCTAACAAG ACCTTGAAAC AAAGGGACTT TGCTCTGAAA 
GATGCTGTTA GTGCATCAAA GCATTCATGC ATTGAAAGCA TCCCCCAAAG TACGACCGAT 
CATCTTGTTG GTGATGATGC CCCTCCTGAA CTTGTTCCCT ATATGGAGCA GATTGAGATG 
GAGGAAGCTC AGAGTTTAGC TGCGGAGATT GTTCGTAGAC ATTTAACAGG TGGCATGCTA 
TCCCACAGTG CTGAATCAGA ATCACATCTC CAAATGGGCT GGGAAAATCT TATTGCTGAG 
CTGAAGAAGC AAGCCAAAGC TCTTCGCAAT ATTTCTCAGG AAATTGAGAA GATCTGCGAG 
ACGGGAGAAA TGAATTCCCT CTCCAGACTT GCTGAGGCAA TCCAATTAAG CTCATACCGC 
AGCGACTCCA GCTCTGTTTG TATGCGAGAG GCTTTAATGC AAGTTCAGGT CGCCTATGTT 
TCTTGCAGGT TGAGAGCGGG CCACACAAGG GAACTTTCCC TGTGCCAGGA GACCAGCCGC 
AACATGTCAG CGCTCGTCCA GGAACATGCA GAGAGTGTTG CTGCCATACA AAAGCACTAC 
CAGAGCTGCT TAGAGAAAGA GCATCTTAGC TTCACAGGCA CCATCAGTTC CTTGCAGGAG 
GAGAATGACA TGCTACGAGG GGAGCTATCC TATAAGCTCA GAGAGCTGCA AGAGCAACGG 
CAGAACCTGT CCCAGCTGGA AGAAGCATTT CATAGGGAGA AAGAAGAGCT GAAGTGCAAA 
CATGCAGATG AATTTGGAAG GGCAGAGCAG GAGCAGATGA CCAGAGAGCT TGAACTAATG 
GACCGTGCTG CCAGTAGCCA ACGCAAACTG GAGACCCTGC TGATAGAAAT GGAAGATTCA 
GAGCTAAAGC ATAAGGAACA AGTCCAAAAA CTCCAGCTTG AATTTCAGGG TAGGATCCAG 
GAGCTGGAGC ACGCCAATAG AGAGGAGCTC TGCAAGCTGA AGAAAAGCTA CAGTCAGACC 
ATCTGCTTTC TGGAGAATAA AGGTGAGGCC GATGCGTGTC CAATGGAGGA AGGCGAAGGA 
TCTGATTCGG TAACATGCAA GGAGTCCCTG CTCCGAGTCC GGGAGCTGGA AATGCAGTTG 
AGCAACATGA GAGAGGAGCT AGAACAGAAA CCACTGGATG GAGATCTGAC CAGCCTGAGG 
GAAAAGTACC AGCGAGACTT GGACAGTCTC AAGGCCACAT GCGAGCGTGG ATTTGCCGCC 
ATGGAGGAGA CGCATCAGAA AGTGATTGAA GACATCCAGA GGCAGCATCA GCGGGAGATC 
CAGAAACTTC TAGAGGAGAA GGAAAGACTG CTGGAGGAGG AAACAAACGC CACTATTGCT 
GCAATTGAGG CCATGAAAAA TGCTCATCGT GAGGAACTGG AGAAAACGCA GCGGTCGCAG 
ATGAGCGGTG TGAGCGCAGA TATTCAGGAG CTGCACAGAC AGTATGAAGA GGAGTTGCAG 
TCTATCCATC GGGAGCTGGA GGTTTTGTCT GAGCAGTATT CTCAGAAGTG TCTGGAAAAC 
GCACATTTGG CTCAAGCGCT GGAAGCTGAG AGACAGGCGC TGGGACAGTG TCAGCGAGAG 
AACCAGAAAC TGCATATACA CAACCAGGAA CTAAACCATC GTCTGAATGA GGAGATCACC 
AGAATGCACT CCTGCATCAG CGGAGAAAAA AACACCTCTT CTCTGACGCA AGGCAAAGAC 
ATCTATGAGC TGGAGGTTTT GCTACGAGTG AAGGAGTCCG AGATCCAGTA CCTCAAACAA 
GAAATAAACT CTCTGAAGGA CGAGCTGCAG TCGGCACTTC GAGATAAGAA GTATGCTTCA 
GATAAATACA AGGACATTTA CACCGAGCTG AGCATTGTGA AAGCTAAAGC TGACTGTGAC 
ATTAACAAGC TGAGGGAGAA ACTTTTGGCT GCTACAGAAG CTCTTGGGGA ACTAGACGCT 
GAGGGAAGCG GTGTTACGGC TGGATACGAC ATTATGAAGT CAAAGAGTAA CCCAGATTTC 
CTGAAGAAGG AAAAATCCAG ACAGATGCGT GGAGTCAGGT CAAAGAGTTT AAAGGAGGGC 
TTGACCGTAC AGGAGAGGAT GAAGCTCTTC GAAGCGAAAG ACTCCAGGAA GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162960.1
CDS	627..7583
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162903.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162903.3

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGCGTTCGCA GGTTATTGAG 
AGATTTGAGG GTCAGGACAC TGTCAACGCT CCAGAGCAAA TGGACACCAC GAGTTCCTCA 
ACAGATCCCG CCCCTTCTTA CTCTAAAACA GCCAATCAGA GAGCAGGACG CAATGAGAAA 
CGCCTCTCCA ACCAGAAACA GGAGTTTTCC TTGGATTCAG CTAAATGCCG GACTGATGAG 
GTTTCTTCAT TAGCTGGTTA CCGGAGGGCC AAAAGTCTGG ATCGCAGAAC CACAGAAACA 
CTCATGACTC CTGACCTGCT GAACTTTAAG AAAGGATGGA TGACCAAACT ATACGAAGAT 
GGATTGTGGA AGAAACACTG GTTTGTTCTG ACCGATCAGA GTCTGAGGTT CTACCGTGAC 
TCCATTGCTG AGGAGGCTTC TGATCTGGAT GGAGAGATCG ATCTGTCCTT GTGTTATGAT 
GTGACCGAAT TCCCAGTTCA GAGGAACTAC GGCTTCCAAA TCCTCAGCAA AGAAGGGGCG 
TTCACACTGT CTGCTATGAC TTCTGGGATA CGTCGTAACT GGATTCAAGC CATTCTGAAA 
AACATGCGGC CCACTGTCGC TCCTGATGTC ACACGGAAAA ACGTCTCTCT GAAACTTTCA 
GTTCTTATGC CCAGTTCCCT TCCGGATGAA CGAACCAGAT CAAATGTGGA GCAGAGCTTC 
ATCCAACCGC CATCACAGCT CTCCAACAGC TCGATAGTTG AGCCAAAAAG GCCACATGAG 
GACATCACCG ACTCCGCACC TCCTCCAGAT CAGCGTAAAA GCAGAGGCCA CAAGCGTGAG 
GGCCGGTCCA AAACCTTCGA CTGGTCAGAA TTTCGACCAG GACAAGAAAA CGAAAGTGGA 
CAACCCTCAC TAAAGAGAGC AGATACTTCA GTCATTATCT CATCTTCTCC ATCATCCATC 
GCTTCCTCTC CCATCTCTAG CTCTTCTTCT CATCAGACGT CTTCCTCCAT CACGATCATC 
AGCCATACTG AAAAGTTGCC ACAGCATAAT GAAGAAGAAG AACAAGTGGT GCAGGAGTAT 
GTACCGCACC ATCAGACTGT ACCTACAACA ATGACCGGTC CAGAGACCCC GTCAAACCCT 
CATAATGTGC CACAGGAACC GGCAGGAATA GATTTGAACC AGTCCAGAGA AGTGCAGGAG 
GTCGATGGTC ATGGAGTGAT CCGCAAAAAC TCTGATGTCC AAGTGGAAAT TGAACAGAGG 
TGGCATCAGG TGGAAACGAC GCCCCTCAGG GAGGAAAAGC AGGTACCGAT CGCTGGTGGC 
TCACCTAACG TATCAAATGT GTCTGTTGGA GAAAGAGCCT CTCCGCAAGA GAAGACCAGC 
AAATTGGAAA AAGAGTTGGG TCAGGCACAA AAGGAGTTGG CCAGACTCCA GGAGCAGAAC 
AGCATCTTAC AAGACCAACT TCAGGATGCA CGCGGGAGAG AGCAGAATGC ACGGGAAGGA 
TATGTACTTC AGAATGACTC GACTATAGCA AAGCCTTCAT CTGATGCTCC CGTGTCTACC 
ACGGTTCATA AAGCACCATG GCAGCGCCTA AGCAAACTAA ACCAGGATCT AAAGTCTGAG 
CTGGAGTCTC AGCGGCGGAA GCAAGATAAG GCCAAACATC AAGTTAGCAG CTTGCGTCGT 
AGCTACAGTG AAGCAAAAGA TATAATAGGG CACCATGAGG CTGAAATCGA GGCCTTGGAA 
GAAAAGCTAA CGGCTGCCAT GGCAGAGATT GTAGTGAGTG AGCAGGCTGT AGCACGAATG 
CGCAGTGATC TGAAAATTGA ACGCGTTCGT TGTCAAGAGC GTGAGGAAGA ATGGACACGA 
AATGAGACAA CATTACGGTC CCAGCTACAA GATAGTGAAG AACGTCTTCG GCAAGTGGAG 
GCCAGTTTGC TTGAGAAGAA TCAAGAGCTG AGGCAGCTGG AGCAGCAGCA AGCTTTACAA 
AGGGACCAGC ACAGAGAGAT GCAGAGGTTA CAGGAGAGAC TCGCTGAGGC TACAGGGCGT 
CTGATTTCCA TGGAGGAGTC TCAGACTATG AGGGAGGAAA GGGAGAGAAA AGAGCACCGG 
AGTCTGGAGG AGAAACATGA GCGGGAACGA CAAGGGCTGA CTCGAAGGTT GGCAGAATCT 
GAAGAAAGGA GACAAGAAAT AGAAGAGCAA TTGCAGGAGG CTCAAGAACA GGTGGAGGCA 
CTGCTGAGGG GCAGCAGTGG GATGGAAACT GTGGGATTGA GGGAGCAAGT GCATCGTCTT 
CAGCAGGAGT TGGAGACACA GACTGATATG GTGGAAATGC TGCGAGAAAG TGTGAGAAGG 
CTGGAGGAGG AACGTGACCA GCTTACTTGC CGCTGTCAGG AACTCCTCAA TCAGATTGCA 
GAGGCTGACA GAGAGGTTGG AAAGCAACAG GCATGTTTAA CAACGGTGGA AACAGACTTT 
CACTCCCTTG AGACCTCATA TGAGCATGTA TCTGAGGAAT TTGCTAGGAT AAGTCATGTG 
CTACAAGAGA AAGAAGAGGA AGTGAGGCAG ACAAAGGAAA TGTATGAGCA TCTTATCAGA 
CAAAAGGAGC AGGACTTGAG TGAAGCCCTC ATCAAGATGG CTGCCTTAGG CAGTAGTTTA 
GAAGAAACAG AACTTTGCTT GCAGCAAAAA GAAGAACTTC TTTCTAAAAT GGAGCGTGAC 
TATCCAGGAC TGGTAGAGTC CCGAAAAGCA GAACAGGAGC TGCAGGCAAA ACTGGTAGTT 
GCAGAGGATC GAATCGCTGA GCTGGAGGAA CACCTCAATG CCCTGCGACT GGGATATGCA 
GATCTCAGAA TGCGACGTTG TCGCTCTCAG GAAGACATGC TTGAGAGTTT AAAGGAGATT 
GAACATGAAG TTTCCCTATC CTCAAGTACA TCCCAGCTTC TTGCTAGAAG CAGTTCAGAG 
ACTGAAATGT CATTAGTAAA GCGACAGAGG ATTCGCTTTT CCACTATCCA GTGCCAAACC 
TACCACCGGT CCCAGGGAGC AGACAAGTTT CAGACAGAAA ACACATCTTT GGATCTTACA 
CAAGATCTGA GTCTAGACCA CATGCATGAC CTGAGCCTGA CTCAAGATAT TAGTGTAGTC 
AGTGACAATT CATTTCAGCA ATGCAGAGAT CCTGAAAAGT TCATCTCCAT CATACATGCT 
CTTGAAACCA AGCTGTTAGC CACTGAGGAG AAACTCCGGC ACCTTACGGA TAAGATACGG 
GACCAGCCCA ATCTTTCAGC GGTGCAGGAA CATCTCAGTA CTAGAGCTAC CATGGAGGAC 
TGGACAACTA AAACCTGTGA TGACTTGCCA ATGGATGAAC CCAGCCAGAA GCACCTAAAC 
AAGACTGTCA GTGAACATGA CAGTGGTGAA TATGAGAAGG CATTGGCACT GGTAGAGTCT 
TGCACTGAAA GAGTCAGAGA GATTCTCAGT AATCAGAATG AGACCAGTTC TGCTGAATCT 
CTGATTTGTA CACTTGCAGA AGTAGAGAAA AGGCTGGTTT GTGCAACTGC ATTCCTAAGG 
AAAGGCCACA CATTATATGA GTCGTGCCAC GTATTTGATG CACCAGATGT GCAGTCAATG 
AAGGGAAGTA TTAAGCTGTT TGCCAGAACA TTGTGTTTTG AGGCTGAAGT TCTGGAGAAG 
ATGGGGTTCT CTTTGCAGGA CTTGAACTCT GATATTATGG CAGCCCTGAA CTCCATTCAC 
AAGGAGGCTG AAAACATCAA GAAGAGCTGT GATGGCTGTC TCTCAGTTGT CTATGCTGAT 
TTGCTTACAA GGAAACTTCT GCTAGAAACC ATGTTCATGT CAGAGATAGA CAAACTTGAG 
ACAGGAAATC TAGCGAATAG TGGTTTTTCA CAGGATGCCA TTAAGAGTGC ATGCATTAGT 
GCTGAGCTTG CATATTCACT TCAGAACCTA ACAACGAATT TCCAGGAAAG ATTTGATGAG 
CTTCAAAAGG ACTTGATTCA GGCTAACAAG ACCTTGAAAC AAAGGGACTT TGCTCTGAAA 
GATGCTGTTA GTGCATCAAA GCATTCATGC ATTGAAAGCA TCCCCCAAAG TACGACCGAT 
CATCTTGTTG GTGATGATGC CCCTCCTGAA CTTGTTCCCT ATATGGAGCA GATTGAGATG 
GAGGAAGCTC AGAGTTTAGC TGCGGAGATT GTTCGTAGAC ATTTAACAGG TGGCATGCTA 
TCCCACAGTG CTGAATCAGA ATCACATCTC CAAATGGGCT GGGAAAATCT TATTGCTGAG 
CTGAAGAAGC AAGCCAAAGC TCTTCGCAAT ATTTCTCAGG AAATTGAGAA GATCTGCGAG 
ACGGGAGAAA TGAATTCCCT CTCCAGACTT GCTGAGGCAA TCCAATTAAG CTCATACCGC 
AGCGACTCCA GCTCTGTTTG TATGCGAGAG GCTTTAATGC AAGTTCAGGT CGCCTATGTT 
TCTTGCAGGT TGAGAGCGGG CCACACAAGG GAACTTTCCC TGTGCCAGGA GACCAGCCGC 
AACATGTCAG CGCTCGTCCA GGAACATGCA GAGAGTGTTG CTGCCATACA AAAGCACTAC 
CAGAGCTGCT TAGAGAAAGA GCATCTTAGC TTCACAGGCA CCATCAGTTC CTTGCAGGAG 
GAGAATGACA TGCTACGAGG GGAGCTATCC TATAAGCTCA GAGAGCTGCA AGAGCAACGG 
CAGAACCTGT CCCAGCTGGA AGAAGCATTT CATAGGGAGA AAGAAGAGCT GAAGTGCAAA 
CATGCAGATG AATTTGGAAG GGCAGAGCAG GAGCAGATGA CCAGAGAGCT TGAACTAATG 
GACCGTGCTG CCAGTAGCCA ACGCAAACTG GAGACCCTGC TGATAGAAAT GGAAGATTCA 
GAGCTAAAGC ATAAGGAACA AGTCCAAAAA CTCCAGCTTG AATTTCAGGG TAGGATCCAG 
GAGCTGGAGC ACGCCAATAG AGAGGAGCTC TGCAAGCTGA AGAAAAGCTA CAGTCAGACC 
ATCTGCTTTC TGGAGAATAA AGGTGAGGCC GATGCGTGTC CAATGGAGGA AGGCGAAGGA 
TCTGATTCGG TAACATGCAA GGAGTCCCTG CTCCGAGTCC GGGAGCTGGA AATGCAGTTG 
AGCAACATGA GAGAGGAGCT AGAACAGAAA CCACTGGATG GAGATCTGAC CAGCCTGAGG 
GAAAAGTACC AGCGAGACTT GGACAGTCTC AAGGCCACAT GCGAGCGTGG ATTTGCCGCC 
ATGGAGGAGA CGCATCAGAA AGTGATTGAA GACATCCAGA GGCAGCATCA GCGGGAGATC 
CAGAAACTTC TAGAGGAGAA GGAAAGACTG CTGGAGGAGG AAACAAACGC CACTATTGCT 
GCAATTGAGG CCATGAAAAA TGCTCATCGT GAGGAACTGG AGAAAACGCA GCGGTCGCAG 
ATGAGCGGTG TGAGCGCAGA TATTCAGGAG CTGCACAGAC AGTATGAAGA GGAGTTGCAG 
TCTATCCATC GGGAGCTGGA GGTTTTGTCT GAGCAGTATT CTCAGAAGTG TCTGGAAAAC 
GCACATTTGG CTCAAGCGCT GGAAGCTGAG AGACAGGCGC TGGGACAGTG TCAGCGAGAG 
AACCAGAAAC TGCATATACA CAACCAGGAA CTAAACCATC GTCTGAATGA GGAGATCACC 
AGAATGCACT CCTGCATCAG CGGAGAAAAA AACACCTCTT CTCTGACGCA AGGCAAAGAC 
ATCTATGAGC TGGAGGTTTT GCTACGAGTG AAGGAGTCCG AGATCCAGTA CCTCAAACAA 
GAAATAAACT CTCTGAAGGA CGAGCTGCAG TCGGCACTTC GAGATAAGAA GTATGCTTCA 
GATAAATACA AGGACATTTA CACCGAGCTG AGCATTGTGA AAGCTAAAGC TGACTGTGAC 
ATTAACAAGC TGAGGGAGAA ACTTTTGGCT GCTACAGAAG CTCTTGGGGA ACTAGACGCT 
GAGGGAAGCG GTGTTACGGC TGGATACGAC ATTATGAAGT CAAAGAGTAA CCCAGATTTC 
CTGAAGAAGG AAAAATCCAG ACAGATGCGT GGAGTCAGGT CAAAGAGTTT AAAGGAGGGC 
TTGACCGTAC AGGAGAGGAT GAAGCTCTTC GAAGCGAAAG ACTCCAGGAA GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch73-103b11.2 ( XM_005162903.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162960.1 Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X5, mRNA.

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CloneID	ODa28904
Clone ID Related Accession (Same CDS sequence)	XM_005162903.3 , XM_005162903.4
Accession Version	XM_005162903.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162903.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGCGTTCGCA GGTTATTGAG 
AGATTTGAGG GTCAGGACAC TGTCAACGCT CCAGAGCAAA TGGACACCAC GAGTTCCTCA 
ACAGATCCCG CCCCTTCTTA CTCTAAAACA GCCAATCAGA GAGCAGGACG CAATGAGAAA 
CGCCTCTCCA ACCAGAAACA GGAGTTTTCC TTGGATTCAG CTAAATGCCG GACTGATGAG 
GTTTCTTCAT TAGCTGGTTA CCGGAGGGCC AAAAGTCTGG ATCGCAGAAC CACAGAAACA 
CTCATGACTC CTGACCTGCT GAACTTTAAG AAAGGATGGA TGACCAAACT ATACGAAGAT 
GGATTGTGGA AGAAACACTG GTTTGTTCTG ACCGATCAGA GTCTGAGGTT CTACCGTGAC 
TCCATTGCTG AGGAGGCTTC TGATCTGGAT GGAGAGATCG ATCTGTCCTT GTGTTATGAT 
GTGACCGAAT TCCCAGTTCA GAGGAACTAC GGCTTCCAAA TCCTCAGCAA AGAAGGGGCG 
TTCACACTGT CTGCTATGAC TTCTGGGATA CGTCGTAACT GGATTCAAGC CATTCTGAAA 
AACATGCGGC CCACTGTCGC TCCTGATGTC ACACGGAAAA ACGTCTCTCT GAAACTTTCA 
GTTCTTATGC CCAGTTCCCT TCCGGATGAA CGAACCAGAT CAAATGTGGA GCAGAGCTTC 
ATCCAACCGC CATCACAGCT CTCCAACAGC TCGATAGTTG AGCCAAAAAG GCCACATGAG 
GACATCACCG ACTCCGCACC TCCTCCAGAT CAGCGTAAAA GCAGAGGCCA CAAGCGTGAG 
GGCCGGTCCA AAACCTTCGA CTGGTCAGAA TTTCGACCAG GACAAGAAAA CGAAAGTGGA 
CAACCCTCAC TAAAGAGAGC AGATACTTCA GTCATTATCT CATCTTCTCC ATCATCCATC 
GCTTCCTCTC CCATCTCTAG CTCTTCTTCT CATCAGACGT CTTCCTCCAT CACGATCATC 
AGCCATACTG AAAAGTTGCC ACAGCATAAT GAAGAAGAAG AACAAGTGGT GCAGGAGTAT 
GTACCGCACC ATCAGACTGT ACCTACAACA ATGACCGGTC CAGAGACCCC GTCAAACCCT 
CATAATGTGC CACAGGAACC GGCAGGAATA GATTTGAACC AGTCCAGAGA AGTGCAGGAG 
GTCGATGGTC ATGGAGTGAT CCGCAAAAAC TCTGATGTCC AAGTGGAAAT TGAACAGAGG 
TGGCATCAGG TGGAAACGAC GCCCCTCAGG GAGGAAAAGC AGGTACCGAT CGCTGGTGGC 
TCACCTAACG TATCAAATGT GTCTGTTGGA GAAAGAGCCT CTCCGCAAGA GAAGACCAGC 
AAATTGGAAA AAGAGTTGGG TCAGGCACAA AAGGAGTTGG CCAGACTCCA GGAGCAGAAC 
AGCATCTTAC AAGACCAACT TCAGGATGCA CGCGGGAGAG AGCAGAATGC ACGGGAAGGA 
TATGTACTTC AGAATGACTC GACTATAGCA AAGCCTTCAT CTGATGCTCC CGTGTCTACC 
ACGGTTCATA AAGCACCATG GCAGCGCCTA AGCAAACTAA ACCAGGATCT AAAGTCTGAG 
CTGGAGTCTC AGCGGCGGAA GCAAGATAAG GCCAAACATC AAGTTAGCAG CTTGCGTCGT 
AGCTACAGTG AAGCAAAAGA TATAATAGGG CACCATGAGG CTGAAATCGA GGCCTTGGAA 
GAAAAGCTAA CGGCTGCCAT GGCAGAGATT GTAGTGAGTG AGCAGGCTGT AGCACGAATG 
CGCAGTGATC TGAAAATTGA ACGCGTTCGT TGTCAAGAGC GTGAGGAAGA ATGGACACGA 
AATGAGACAA CATTACGGTC CCAGCTACAA GATAGTGAAG AACGTCTTCG GCAAGTGGAG 
GCCAGTTTGC TTGAGAAGAA TCAAGAGCTG AGGCAGCTGG AGCAGCAGCA AGCTTTACAA 
AGGGACCAGC ACAGAGAGAT GCAGAGGTTA CAGGAGAGAC TCGCTGAGGC TACAGGGCGT 
CTGATTTCCA TGGAGGAGTC TCAGACTATG AGGGAGGAAA GGGAGAGAAA AGAGCACCGG 
AGTCTGGAGG AGAAACATGA GCGGGAACGA CAAGGGCTGA CTCGAAGGTT GGCAGAATCT 
GAAGAAAGGA GACAAGAAAT AGAAGAGCAA TTGCAGGAGG CTCAAGAACA GGTGGAGGCA 
CTGCTGAGGG GCAGCAGTGG GATGGAAACT GTGGGATTGA GGGAGCAAGT GCATCGTCTT 
CAGCAGGAGT TGGAGACACA GACTGATATG GTGGAAATGC TGCGAGAAAG TGTGAGAAGG 
CTGGAGGAGG AACGTGACCA GCTTACTTGC CGCTGTCAGG AACTCCTCAA TCAGATTGCA 
GAGGCTGACA GAGAGGTTGG AAAGCAACAG GCATGTTTAA CAACGGTGGA AACAGACTTT 
CACTCCCTTG AGACCTCATA TGAGCATGTA TCTGAGGAAT TTGCTAGGAT AAGTCATGTG 
CTACAAGAGA AAGAAGAGGA AGTGAGGCAG ACAAAGGAAA TGTATGAGCA TCTTATCAGA 
CAAAAGGAGC AGGACTTGAG TGAAGCCCTC ATCAAGATGG CTGCCTTAGG CAGTAGTTTA 
GAAGAAACAG AACTTTGCTT GCAGCAAAAA GAAGAACTTC TTTCTAAAAT GGAGCGTGAC 
TATCCAGGAC TGGTAGAGTC CCGAAAAGCA GAACAGGAGC TGCAGGCAAA ACTGGTAGTT 
GCAGAGGATC GAATCGCTGA GCTGGAGGAA CACCTCAATG CCCTGCGACT GGGATATGCA 
GATCTCAGAA TGCGACGTTG TCGCTCTCAG GAAGACATGC TTGAGAGTTT AAAGGAGATT 
GAACATGAAG TTTCCCTATC CTCAAGTACA TCCCAGCTTC TTGCTAGAAG CAGTTCAGAG 
ACTGAAATGT CATTAGTAAA GCGACAGAGG ATTCGCTTTT CCACTATCCA GTGCCAAACC 
TACCACCGGT CCCAGGGAGC AGACAAGTTT CAGACAGAAA ACACATCTTT GGATCTTACA 
CAAGATCTGA GTCTAGACCA CATGCATGAC CTGAGCCTGA CTCAAGATAT TAGTGTAGTC 
AGTGACAATT CATTTCAGCA ATGCAGAGAT CCTGAAAAGT TCATCTCCAT CATACATGCT 
CTTGAAACCA AGCTGTTAGC CACTGAGGAG AAACTCCGGC ACCTTACGGA TAAGATACGG 
GACCAGCCCA ATCTTTCAGC GGTGCAGGAA CATCTCAGTA CTAGAGCTAC CATGGAGGAC 
TGGACAACTA AAACCTGTGA TGACTTGCCA ATGGATGAAC CCAGCCAGAA GCACCTAAAC 
AAGACTGTCA GTGAACATGA CAGTGGTGAA TATGAGAAGG CATTGGCACT GGTAGAGTCT 
TGCACTGAAA GAGTCAGAGA GATTCTCAGT AATCAGAATG AGACCAGTTC TGCTGAATCT 
CTGATTTGTA CACTTGCAGA AGTAGAGAAA AGGCTGGTTT GTGCAACTGC ATTCCTAAGG 
AAAGGCCACA CATTATATGA GTCGTGCCAC GTATTTGATG CACCAGATGT GCAGTCAATG 
AAGGGAAGTA TTAAGCTGTT TGCCAGAACA TTGTGTTTTG AGGCTGAAGT TCTGGAGAAG 
ATGGGGTTCT CTTTGCAGGA CTTGAACTCT GATATTATGG CAGCCCTGAA CTCCATTCAC 
AAGGAGGCTG AAAACATCAA GAAGAGCTGT GATGGCTGTC TCTCAGTTGT CTATGCTGAT 
TTGCTTACAA GGAAACTTCT GCTAGAAACC ATGTTCATGT CAGAGATAGA CAAACTTGAG 
ACAGGAAATC TAGCGAATAG TGGTTTTTCA CAGGATGCCA TTAAGAGTGC ATGCATTAGT 
GCTGAGCTTG CATATTCACT TCAGAACCTA ACAACGAATT TCCAGGAAAG ATTTGATGAG 
CTTCAAAAGG ACTTGATTCA GGCTAACAAG ACCTTGAAAC AAAGGGACTT TGCTCTGAAA 
GATGCTGTTA GTGCATCAAA GCATTCATGC ATTGAAAGCA TCCCCCAAAG TACGACCGAT 
CATCTTGTTG GTGATGATGC CCCTCCTGAA CTTGTTCCCT ATATGGAGCA GATTGAGATG 
GAGGAAGCTC AGAGTTTAGC TGCGGAGATT GTTCGTAGAC ATTTAACAGG TGGCATGCTA 
TCCCACAGTG CTGAATCAGA ATCACATCTC CAAATGGGCT GGGAAAATCT TATTGCTGAG 
CTGAAGAAGC AAGCCAAAGC TCTTCGCAAT ATTTCTCAGG AAATTGAGAA GATCTGCGAG 
ACGGGAGAAA TGAATTCCCT CTCCAGACTT GCTGAGGCAA TCCAATTAAG CTCATACCGC 
AGCGACTCCA GCTCTGTTTG TATGCGAGAG GCTTTAATGC AAGTTCAGGT CGCCTATGTT 
TCTTGCAGGT TGAGAGCGGG CCACACAAGG GAACTTTCCC TGTGCCAGGA GACCAGCCGC 
AACATGTCAG CGCTCGTCCA GGAACATGCA GAGAGTGTTG CTGCCATACA AAAGCACTAC 
CAGAGCTGCT TAGAGAAAGA GCATCTTAGC TTCACAGGCA CCATCAGTTC CTTGCAGGAG 
GAGAATGACA TGCTACGAGG GGAGCTATCC TATAAGCTCA GAGAGCTGCA AGAGCAACGG 
CAGAACCTGT CCCAGCTGGA AGAAGCATTT CATAGGGAGA AAGAAGAGCT GAAGTGCAAA 
CATGCAGATG AATTTGGAAG GGCAGAGCAG GAGCAGATGA CCAGAGAGCT TGAACTAATG 
GACCGTGCTG CCAGTAGCCA ACGCAAACTG GAGACCCTGC TGATAGAAAT GGAAGATTCA 
GAGCTAAAGC ATAAGGAACA AGTCCAAAAA CTCCAGCTTG AATTTCAGGG TAGGATCCAG 
GAGCTGGAGC ACGCCAATAG AGAGGAGCTC TGCAAGCTGA AGAAAAGCTA CAGTCAGACC 
ATCTGCTTTC TGGAGAATAA AGGTGAGGCC GATGCGTGTC CAATGGAGGA AGGCGAAGGA 
TCTGATTCGG TAACATGCAA GGAGTCCCTG CTCCGAGTCC GGGAGCTGGA AATGCAGTTG 
AGCAACATGA GAGAGGAGCT AGAACAGAAA CCACTGGATG GAGATCTGAC CAGCCTGAGG 
GAAAAGTACC AGCGAGACTT GGACAGTCTC AAGGCCACAT GCGAGCGTGG ATTTGCCGCC 
ATGGAGGAGA CGCATCAGAA AGTGATTGAA GACATCCAGA GGCAGCATCA GCGGGAGATC 
CAGAAACTTC TAGAGGAGAA GGAAAGACTG CTGGAGGAGG AAACAAACGC CACTATTGCT 
GCAATTGAGG CCATGAAAAA TGCTCATCGT GAGGAACTGG AGAAAACGCA GCGGTCGCAG 
ATGAGCGGTG TGAGCGCAGA TATTCAGGAG CTGCACAGAC AGTATGAAGA GGAGTTGCAG 
TCTATCCATC GGGAGCTGGA GGTTTTGTCT GAGCAGTATT CTCAGAAGTG TCTGGAAAAC 
GCACATTTGG CTCAAGCGCT GGAAGCTGAG AGACAGGCGC TGGGACAGTG TCAGCGAGAG 
AACCAGAAAC TGCATATACA CAACCAGGAA CTAAACCATC GTCTGAATGA GGAGATCACC 
AGAATGCACT CCTGCATCAG CGGAGAAAAA AACACCTCTT CTCTGACGCA AGGCAAAGAC 
ATCTATGAGC TGGAGGTTTT GCTACGAGTG AAGGAGTCCG AGATCCAGTA CCTCAAACAA 
GAAATAAACT CTCTGAAGGA CGAGCTGCAG TCGGCACTTC GAGATAAGAA GTATGCTTCA 
GATAAATACA AGGACATTTA CACCGAGCTG AGCATTGTGA AAGCTAAAGC TGACTGTGAC 
ATTAACAAGC TGAGGGAGAA ACTTTTGGCT GCTACAGAAG CTCTTGGGGA ACTAGACGCT 
GAGGGAAGCG GTGTTACGGC TGGATACGAC ATTATGAAGT CAAAGAGTAA CCCAGATTTC 
CTGAAGAAGG AAAAATCCAG ACAGATGCGT GGAGTCAGGT CAAAGAGTTT AAAGGAGGGC 
TTGACCGTAC AGGAGAGGAT GAAGCTCTTC GAAGCGAAAG ACTCCAGGAA GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162960.1
CDS	634..7590
Translation	MAQSTSAKDNACRKFQANIFNKSKCQNCFKARDAHLLSDEDFTQAKPIYGGWLLLAPEGTNFDNPLHRSRKWQRRFFLLYEHGLLRYALDDMASTLPQGTINLNQCVDVVDGESRTGQKHSLCICTPDKDHYIRAESKETIHGWQEALMVFPRTNKQNQKKKRKVETPTPQEPGPAKVTVTSTSSSSSMVCLPSTIAHAERVPATRTTLWQEETWSRATLPTSCSTGSLSQLDSGNSNVGRKPRVESGYFSLEKPKIEDEGQQYHLQQEEEPPSKLQSHPPELSSSSSSSSRSRRSQVIERFEGQDTVNAPEQMDTTSSSTDPAPSYSKTANQRAGRNEKRLSNQKQEFSLDSAKCRTDEVSSLAGYRRAKSLDRRTTETLMTPDLLNFKKGWMTKLYEDGLWKKHWFVLTDQSLRFYRDSIAEEASDLDGEIDLSLCYDVTEFPVQRNYGFQILSKEGAFTLSAMTSGIRRNWIQAILKNMRPTVAPDVTRKNVSLKLSVLMPSSLPDERTRSNVEQSFIQPPSQLSNSSIVEPKRPHEDITDSAPPPDQRKSRGHKREGRSKTFDWSEFRPGQENESGQPSLKRADTSVIISSSPSSIASSPISSSSSHQTSSSITIISHTEKLPQHNEEEEQVVQEYVPHHQTVPTTMTGPETPSNPHNVPQEPAGIDLNQSREVQEVDGHGVIRKNSDVQVEIEQRWHQVETTPLREEKQVPIAGGSPNVSNVSVGERASPQEKTSKLEKELGQAQKELARLQEQNSILQDQLQDARGREQNAREGYVLQNDSTIAKPSSDAPVSTTVHKAPWQRLSKLNQDLKSELESQRRKQDKAKHQVSSLRRSYSEAKDIIGHHEAEIEALEEKLTAAMAEIVVSEQAVARMRSDLKIERVRCQEREEEWTRNETTLRSQLQDSEERLRQVEASLLEKNQELRQLEQQQALQRDQHREMQRLQERLAEATGRLISMEESQTMREERERKEHRSLEEKHERERQGLTRRLAESEERRQEIEEQLQEAQEQVEALLRGSSGMETVGLREQVHRLQQELETQTDMVEMLRESVRRLEEERDQLTCRCQELLNQIAEADREVGKQQACLTTVETDFHSLETSYEHVSEEFARISHVLQEKEEEVRQTKEMYEHLIRQKEQDLSEALIKMAALGSSLEETELCLQQKEELLSKMERDYPGLVESRKAEQELQAKLVVAEDRIAELEEHLNALRLGYADLRMRRCRSQEDMLESLKEIEHEVSLSSSTSQLLARSSSETEMSLVKRQRIRFSTIQCQTYHRSQGADKFQTENTSLDLTQDLSLDHMHDLSLTQDISVVSDNSFQQCRDPEKFISIIHALETKLLATEEKLRHLTDKIRDQPNLSAVQEHLSTRATMEDWTTKTCDDLPMDEPSQKHLNKTVSEHDSGEYEKALALVESCTERVREILSNQNETSSAESLICTLAEVEKRLVCATAFLRKGHTLYESCHVFDAPDVQSMKGSIKLFARTLCFEAEVLEKMGFSLQDLNSDIMAALNSIHKEAENIKKSCDGCLSVVYADLLTRKLLLETMFMSEIDKLETGNLANSGFSQDAIKSACISAELAYSLQNLTTNFQERFDELQKDLIQANKTLKQRDFALKDAVSASKHSCIESIPQSTTDHLVGDDAPPELVPYMEQIEMEEAQSLAAEIVRRHLTGGMLSHSAESESHLQMGWENLIAELKKQAKALRNISQEIEKICETGEMNSLSRLAEAIQLSSYRSDSSSVCMREALMQVQVAYVSCRLRAGHTRELSLCQETSRNMSALVQEHAESVAAIQKHYQSCLEKEHLSFTGTISSLQEENDMLRGELSYKLRELQEQRQNLSQLEEAFHREKEELKCKHADEFGRAEQEQMTRELELMDRAASSQRKLETLLIEMEDSELKHKEQVQKLQLEFQGRIQELEHANREELCKLKKSYSQTICFLENKGEADACPMEEGEGSDSVTCKESLLRVRELEMQLSNMREELEQKPLDGDLTSLREKYQRDLDSLKATCERGFAAMEETHQKVIEDIQRQHQREIQKLLEEKERLLEEETNATIAAIEAMKNAHREELEKTQRSQMSGVSADIQELHRQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALGQCQRENQKLHIHNQELNHRLNEEITRMHSCISGEKNTSSLTQGKDIYELEVLLRVKESEIQYLKQEINSLKDELQSALRDKKYASDKYKDIYTELSIVKAKADCDINKLREKLLAATEALGELDAEGSGVTAGYDIMKSKSNPDFLKKEKSRQMRGVRSKSLKEGLTVQERMKLFEAKDSRKI

Target ORF information:

RefSeq Version	XM_005162903.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch73-103b11.2 (si:ch73-103b11.2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162903.4

ORF Insert Sequence:

ATGGCACAGA GCACGTCTGC GAAAGATAAC GCCTGTCGAA AATTCCAGGC CAATATTTTC 
AACAAGAGTA AATGCCAGAA CTGCTTCAAA GCTCGAGACG CGCACCTGCT GAGTGATGAA 
GACTTCACAC AGGCAAAGCC CATCTACGGT GGATGGCTGC TCCTGGCACC TGAAGGAACC 
AACTTTGACA ATCCTCTGCA CAGATCACGG AAATGGCAGA GACGTTTTTT CCTTCTGTAT 
GAACATGGAC TCCTGCGTTA TGCGCTGGAT GATATGGCCA GCACTCTTCC ACAGGGCACC 
ATAAACCTGA ATCAGTGTGT GGATGTGGTG GACGGTGAAA GCCGGACGGG TCAGAAACAC 
TCGCTCTGCA TCTGCACACC AGACAAAGAC CATTACATCC GCGCTGAGAG CAAAGAGACA 
ATACATGGAT GGCAGGAGGC GCTGATGGTG TTTCCACGAA CCAACAAACA AAACCAGAAG 
AAGAAACGGA AAGTAGAAAC GCCAACACCA CAGGAACCAG GCCCAGCGAA GGTGACGGTC 
ACCAGTACGA GCAGCAGCAG CAGTATGGTG TGTCTGCCTA GCACTATAGC TCATGCAGAG 
CGAGTGCCGG CCACTCGAAC CACACTGTGG CAGGAGGAAA CATGGAGTCG AGCCACTTTA 
CCCACAAGCT GCAGCACCGG CAGCCTGAGT CAACTGGATT CTGGAAACTC GAATGTTGGT 
CGCAAGCCTC GCGTGGAGAG TGGATATTTT TCACTGGAAA AACCCAAGAT TGAAGATGAA 
GGACAGCAGT ATCACCTTCA ACAGGAGGAA GAGCCTCCAT CAAAGCTGCA ATCACATCCA 
CCTGAGCTTT CCTCTTCGTC ATCATCATCC TCTCGCTCCA GGCGTTCGCA GGTTATTGAG 
AGATTTGAGG GTCAGGACAC TGTCAACGCT CCAGAGCAAA TGGACACCAC GAGTTCCTCA 
ACAGATCCCG CCCCTTCTTA CTCTAAAACA GCCAATCAGA GAGCAGGACG CAATGAGAAA 
CGCCTCTCCA ACCAGAAACA GGAGTTTTCC TTGGATTCAG CTAAATGCCG GACTGATGAG 
GTTTCTTCAT TAGCTGGTTA CCGGAGGGCC AAAAGTCTGG ATCGCAGAAC CACAGAAACA 
CTCATGACTC CTGACCTGCT GAACTTTAAG AAAGGATGGA TGACCAAACT ATACGAAGAT 
GGATTGTGGA AGAAACACTG GTTTGTTCTG ACCGATCAGA GTCTGAGGTT CTACCGTGAC 
TCCATTGCTG AGGAGGCTTC TGATCTGGAT GGAGAGATCG ATCTGTCCTT GTGTTATGAT 
GTGACCGAAT TCCCAGTTCA GAGGAACTAC GGCTTCCAAA TCCTCAGCAA AGAAGGGGCG 
TTCACACTGT CTGCTATGAC TTCTGGGATA CGTCGTAACT GGATTCAAGC CATTCTGAAA 
AACATGCGGC CCACTGTCGC TCCTGATGTC ACACGGAAAA ACGTCTCTCT GAAACTTTCA 
GTTCTTATGC CCAGTTCCCT TCCGGATGAA CGAACCAGAT CAAATGTGGA GCAGAGCTTC 
ATCCAACCGC CATCACAGCT CTCCAACAGC TCGATAGTTG AGCCAAAAAG GCCACATGAG 
GACATCACCG ACTCCGCACC TCCTCCAGAT CAGCGTAAAA GCAGAGGCCA CAAGCGTGAG 
GGCCGGTCCA AAACCTTCGA CTGGTCAGAA TTTCGACCAG GACAAGAAAA CGAAAGTGGA 
CAACCCTCAC TAAAGAGAGC AGATACTTCA GTCATTATCT CATCTTCTCC ATCATCCATC 
GCTTCCTCTC CCATCTCTAG CTCTTCTTCT CATCAGACGT CTTCCTCCAT CACGATCATC 
AGCCATACTG AAAAGTTGCC ACAGCATAAT GAAGAAGAAG AACAAGTGGT GCAGGAGTAT 
GTACCGCACC ATCAGACTGT ACCTACAACA ATGACCGGTC CAGAGACCCC GTCAAACCCT 
CATAATGTGC CACAGGAACC GGCAGGAATA GATTTGAACC AGTCCAGAGA AGTGCAGGAG 
GTCGATGGTC ATGGAGTGAT CCGCAAAAAC TCTGATGTCC AAGTGGAAAT TGAACAGAGG 
TGGCATCAGG TGGAAACGAC GCCCCTCAGG GAGGAAAAGC AGGTACCGAT CGCTGGTGGC 
TCACCTAACG TATCAAATGT GTCTGTTGGA GAAAGAGCCT CTCCGCAAGA GAAGACCAGC 
AAATTGGAAA AAGAGTTGGG TCAGGCACAA AAGGAGTTGG CCAGACTCCA GGAGCAGAAC 
AGCATCTTAC AAGACCAACT TCAGGATGCA CGCGGGAGAG AGCAGAATGC ACGGGAAGGA 
TATGTACTTC AGAATGACTC GACTATAGCA AAGCCTTCAT CTGATGCTCC CGTGTCTACC 
ACGGTTCATA AAGCACCATG GCAGCGCCTA AGCAAACTAA ACCAGGATCT AAAGTCTGAG 
CTGGAGTCTC AGCGGCGGAA GCAAGATAAG GCCAAACATC AAGTTAGCAG CTTGCGTCGT 
AGCTACAGTG AAGCAAAAGA TATAATAGGG CACCATGAGG CTGAAATCGA GGCCTTGGAA 
GAAAAGCTAA CGGCTGCCAT GGCAGAGATT GTAGTGAGTG AGCAGGCTGT AGCACGAATG 
CGCAGTGATC TGAAAATTGA ACGCGTTCGT TGTCAAGAGC GTGAGGAAGA ATGGACACGA 
AATGAGACAA CATTACGGTC CCAGCTACAA GATAGTGAAG AACGTCTTCG GCAAGTGGAG 
GCCAGTTTGC TTGAGAAGAA TCAAGAGCTG AGGCAGCTGG AGCAGCAGCA AGCTTTACAA 
AGGGACCAGC ACAGAGAGAT GCAGAGGTTA CAGGAGAGAC TCGCTGAGGC TACAGGGCGT 
CTGATTTCCA TGGAGGAGTC TCAGACTATG AGGGAGGAAA GGGAGAGAAA AGAGCACCGG 
AGTCTGGAGG AGAAACATGA GCGGGAACGA CAAGGGCTGA CTCGAAGGTT GGCAGAATCT 
GAAGAAAGGA GACAAGAAAT AGAAGAGCAA TTGCAGGAGG CTCAAGAACA GGTGGAGGCA 
CTGCTGAGGG GCAGCAGTGG GATGGAAACT GTGGGATTGA GGGAGCAAGT GCATCGTCTT 
CAGCAGGAGT TGGAGACACA GACTGATATG GTGGAAATGC TGCGAGAAAG TGTGAGAAGG 
CTGGAGGAGG AACGTGACCA GCTTACTTGC CGCTGTCAGG AACTCCTCAA TCAGATTGCA 
GAGGCTGACA GAGAGGTTGG AAAGCAACAG GCATGTTTAA CAACGGTGGA AACAGACTTT 
CACTCCCTTG AGACCTCATA TGAGCATGTA TCTGAGGAAT TTGCTAGGAT AAGTCATGTG 
CTACAAGAGA AAGAAGAGGA AGTGAGGCAG ACAAAGGAAA TGTATGAGCA TCTTATCAGA 
CAAAAGGAGC AGGACTTGAG TGAAGCCCTC ATCAAGATGG CTGCCTTAGG CAGTAGTTTA 
GAAGAAACAG AACTTTGCTT GCAGCAAAAA GAAGAACTTC TTTCTAAAAT GGAGCGTGAC 
TATCCAGGAC TGGTAGAGTC CCGAAAAGCA GAACAGGAGC TGCAGGCAAA ACTGGTAGTT 
GCAGAGGATC GAATCGCTGA GCTGGAGGAA CACCTCAATG CCCTGCGACT GGGATATGCA 
GATCTCAGAA TGCGACGTTG TCGCTCTCAG GAAGACATGC TTGAGAGTTT AAAGGAGATT 
GAACATGAAG TTTCCCTATC CTCAAGTACA TCCCAGCTTC TTGCTAGAAG CAGTTCAGAG 
ACTGAAATGT CATTAGTAAA GCGACAGAGG ATTCGCTTTT CCACTATCCA GTGCCAAACC 
TACCACCGGT CCCAGGGAGC AGACAAGTTT CAGACAGAAA ACACATCTTT GGATCTTACA 
CAAGATCTGA GTCTAGACCA CATGCATGAC CTGAGCCTGA CTCAAGATAT TAGTGTAGTC 
AGTGACAATT CATTTCAGCA ATGCAGAGAT CCTGAAAAGT TCATCTCCAT CATACATGCT 
CTTGAAACCA AGCTGTTAGC CACTGAGGAG AAACTCCGGC ACCTTACGGA TAAGATACGG 
GACCAGCCCA ATCTTTCAGC GGTGCAGGAA CATCTCAGTA CTAGAGCTAC CATGGAGGAC 
TGGACAACTA AAACCTGTGA TGACTTGCCA ATGGATGAAC CCAGCCAGAA GCACCTAAAC 
AAGACTGTCA GTGAACATGA CAGTGGTGAA TATGAGAAGG CATTGGCACT GGTAGAGTCT 
TGCACTGAAA GAGTCAGAGA GATTCTCAGT AATCAGAATG AGACCAGTTC TGCTGAATCT 
CTGATTTGTA CACTTGCAGA AGTAGAGAAA AGGCTGGTTT GTGCAACTGC ATTCCTAAGG 
AAAGGCCACA CATTATATGA GTCGTGCCAC GTATTTGATG CACCAGATGT GCAGTCAATG 
AAGGGAAGTA TTAAGCTGTT TGCCAGAACA TTGTGTTTTG AGGCTGAAGT TCTGGAGAAG 
ATGGGGTTCT CTTTGCAGGA CTTGAACTCT GATATTATGG CAGCCCTGAA CTCCATTCAC 
AAGGAGGCTG AAAACATCAA GAAGAGCTGT GATGGCTGTC TCTCAGTTGT CTATGCTGAT 
TTGCTTACAA GGAAACTTCT GCTAGAAACC ATGTTCATGT CAGAGATAGA CAAACTTGAG 
ACAGGAAATC TAGCGAATAG TGGTTTTTCA CAGGATGCCA TTAAGAGTGC ATGCATTAGT 
GCTGAGCTTG CATATTCACT TCAGAACCTA ACAACGAATT TCCAGGAAAG ATTTGATGAG 
CTTCAAAAGG ACTTGATTCA GGCTAACAAG ACCTTGAAAC AAAGGGACTT TGCTCTGAAA 
GATGCTGTTA GTGCATCAAA GCATTCATGC ATTGAAAGCA TCCCCCAAAG TACGACCGAT 
CATCTTGTTG GTGATGATGC CCCTCCTGAA CTTGTTCCCT ATATGGAGCA GATTGAGATG 
GAGGAAGCTC AGAGTTTAGC TGCGGAGATT GTTCGTAGAC ATTTAACAGG TGGCATGCTA 
TCCCACAGTG CTGAATCAGA ATCACATCTC CAAATGGGCT GGGAAAATCT TATTGCTGAG 
CTGAAGAAGC AAGCCAAAGC TCTTCGCAAT ATTTCTCAGG AAATTGAGAA GATCTGCGAG 
ACGGGAGAAA TGAATTCCCT CTCCAGACTT GCTGAGGCAA TCCAATTAAG CTCATACCGC 
AGCGACTCCA GCTCTGTTTG TATGCGAGAG GCTTTAATGC AAGTTCAGGT CGCCTATGTT 
TCTTGCAGGT TGAGAGCGGG CCACACAAGG GAACTTTCCC TGTGCCAGGA GACCAGCCGC 
AACATGTCAG CGCTCGTCCA GGAACATGCA GAGAGTGTTG CTGCCATACA AAAGCACTAC 
CAGAGCTGCT TAGAGAAAGA GCATCTTAGC TTCACAGGCA CCATCAGTTC CTTGCAGGAG 
GAGAATGACA TGCTACGAGG GGAGCTATCC TATAAGCTCA GAGAGCTGCA AGAGCAACGG 
CAGAACCTGT CCCAGCTGGA AGAAGCATTT CATAGGGAGA AAGAAGAGCT GAAGTGCAAA 
CATGCAGATG AATTTGGAAG GGCAGAGCAG GAGCAGATGA CCAGAGAGCT TGAACTAATG 
GACCGTGCTG CCAGTAGCCA ACGCAAACTG GAGACCCTGC TGATAGAAAT GGAAGATTCA 
GAGCTAAAGC ATAAGGAACA AGTCCAAAAA CTCCAGCTTG AATTTCAGGG TAGGATCCAG 
GAGCTGGAGC ACGCCAATAG AGAGGAGCTC TGCAAGCTGA AGAAAAGCTA CAGTCAGACC 
ATCTGCTTTC TGGAGAATAA AGGTGAGGCC GATGCGTGTC CAATGGAGGA AGGCGAAGGA 
TCTGATTCGG TAACATGCAA GGAGTCCCTG CTCCGAGTCC GGGAGCTGGA AATGCAGTTG 
AGCAACATGA GAGAGGAGCT AGAACAGAAA CCACTGGATG GAGATCTGAC CAGCCTGAGG 
GAAAAGTACC AGCGAGACTT GGACAGTCTC AAGGCCACAT GCGAGCGTGG ATTTGCCGCC 
ATGGAGGAGA CGCATCAGAA AGTGATTGAA GACATCCAGA GGCAGCATCA GCGGGAGATC 
CAGAAACTTC TAGAGGAGAA GGAAAGACTG CTGGAGGAGG AAACAAACGC CACTATTGCT 
GCAATTGAGG CCATGAAAAA TGCTCATCGT GAGGAACTGG AGAAAACGCA GCGGTCGCAG 
ATGAGCGGTG TGAGCGCAGA TATTCAGGAG CTGCACAGAC AGTATGAAGA GGAGTTGCAG 
TCTATCCATC GGGAGCTGGA GGTTTTGTCT GAGCAGTATT CTCAGAAGTG TCTGGAAAAC 
GCACATTTGG CTCAAGCGCT GGAAGCTGAG AGACAGGCGC TGGGACAGTG TCAGCGAGAG 
AACCAGAAAC TGCATATACA CAACCAGGAA CTAAACCATC GTCTGAATGA GGAGATCACC 
AGAATGCACT CCTGCATCAG CGGAGAAAAA AACACCTCTT CTCTGACGCA AGGCAAAGAC 
ATCTATGAGC TGGAGGTTTT GCTACGAGTG AAGGAGTCCG AGATCCAGTA CCTCAAACAA 
GAAATAAACT CTCTGAAGGA CGAGCTGCAG TCGGCACTTC GAGATAAGAA GTATGCTTCA 
GATAAATACA AGGACATTTA CACCGAGCTG AGCATTGTGA AAGCTAAAGC TGACTGTGAC 
ATTAACAAGC TGAGGGAGAA ACTTTTGGCT GCTACAGAAG CTCTTGGGGA ACTAGACGCT 
GAGGGAAGCG GTGTTACGGC TGGATACGAC ATTATGAAGT CAAAGAGTAA CCCAGATTTC 
CTGAAGAAGG AAAAATCCAG ACAGATGCGT GGAGTCAGGT CAAAGAGTTT AAAGGAGGGC 
TTGACCGTAC AGGAGAGGAT GAAGCTCTTC GAAGCGAAAG ACTCCAGGAA GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	si:ch73-103b11.2
Entrez Gene ID	562530
Full Name	si:ch73-103b11.2
Synonyms	im:7154419
General protein information	Preferred Names si:ch73-103b11.2 Names centrosome-associated protein CEP250 early endosome antigen 1 myosin phosphatase Rho-interacting protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Canis lupus familiaris (dog)	LOC479530	XP_005620232.1
Rattus norvegicus (Norway rat)	Mprip	NP_446266.2
Xenopus tropicalis (tropical clawed frog)	mprip	XP_004918142.1
Homo sapiens (human)	MPRIP	NP_055949.2
Mus musculus (house mouse)	Mprip	NP_957697.1
Gallus gallus (chicken)	MPRIP	XP_004945241.1
Danio rerio (zebrafish)	si:ch73-103b11.2	XP_005162957.1
Pan troglodytes (chimpanzee)	MPRIP	XP_001160793.1
Bos taurus (cattle)	MPRIP	NP_001099097.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

si:ch73-103b11.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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