usp31 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp31
Entrez Gene ID	564487
Full Name	ubiquitin specific peptidase 31
General protein information	Preferred Names ubiquitin specific peptidase 31 Names ubiquitin carboxyl-terminal hydrolase 31
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164228.3	XP_005164285.1	ubiquitin carboxyl-terminal hydrolase 31 isoform X1
XM_005164229.4	XP_005164286.1	ubiquitin carboxyl-terminal hydrolase 31 isoform X2
XM_005164229.3	XP_005164286.1	ubiquitin carboxyl-terminal hydrolase 31 isoform X2
XM_005164228.4	XP_005164285.1	ubiquitin carboxyl-terminal hydrolase 31 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP31	NP_065769.3
Canis lupus familiaris (dog)	USP31	XP_005622126.1
Mus musculus (house mouse)	Usp31	NP_001028345.1
Gallus gallus (chicken)	USP31	XP_003642194.2
Xenopus tropicalis (tropical clawed frog)	usp31	XP_002932037.2
Rattus norvegicus (Norway rat)	Usp31	NP_001101018.1
Pan troglodytes (chimpanzee)	USP31	XP_003315066.1
	USP31	XP_001087999.2
Bos taurus (cattle)	USP31	XP_002698069.1
Danio rerio (zebrafish)	usp31	XP_005164286.1

Gene Ontology

The following usp31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08711	XM_005164228.4 Latest version!	Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa08712	XM_005164229.4 Latest version!	Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa08712	XM_005164229.3	Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa08711	XM_005164228.3	Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp31 ( XM_005164228.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164285.1 Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08711
Clone ID Related Accession (Same CDS sequence)	XM_005164228.3 , XM_005164228.4
Accession Version	XM_005164228.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 31 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164228.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AATCTTCCAG CAAAGAGAAG AAGTCCGGCT TCAGCAAGAA GCTGTTCCGC 
CGGGGTTCGG TGCGCTCCGT GGGCAGTTTT ATGAGCAGGG TCCTCAAGAC CCTCTCAACA 
CTCTCTCACT TCGGCTCTGA GCTGCACGCG CCCGATGGAG AAAAGGACGA CGGCGGATTC 
ACATCCTTCA AGAACGAAGG AAAGGGCTCA GTCGCGTCGG ACGAGAGCGA CTGCGGGGGC 
TTTCTGTCCA GGGACAAGAT CCCCGGCGTG GCCGGTTTGA AAAACCACGG CAATACGTGT 
TTCATGAACG CTATTCTGCA GTGCCTGAGC AACACAGAGC TGTTCGCCGA GTATTTGGCC 
CTGGAACAGT ACCGAGGAGA TCAGACTGAC GACGAGAAGC CCAAGACCAA CGGGGTCGTG 
GGCAGAAAGG GCGCTCAGGA CCGGGGAGAG GTGACCGAAC AGCTGTCCGG GCTGGTCCGA 
GCCTTGTGGA CATTTGAGTA CACACCGCAG CACAGTCGAG ACTTCAAAAA TGCTGTTTCT 
AGGAGTGCCA TGCAATATCG GGGAAATGCA CAACACGATG CCCAGGAGTT TCTTCTCTGG 
TTACTGGACC GCGTCCATGA AGACCTCAAC CATCTGGTAC ACCCCAACAT CAGAGGATCT 
GTTAAGCCAC CCACAGAGGA AGACGATGCA GCTTTAGAGG GACCTTCCCC TCCTCTGTCA 
GCCGGCTCAT TTGTCCAGGA GCTGTTTCAA GCTCAGTACA GATCCTCGCT AACCTGTCCA 
CACTGTCAGA AACAGAGCAA CACTTTTGAT CCTTTTCTGT GTATCTCCCT CCCGATTCCA 
TTACCTCACA CACGGCCTCT GTATGTGACT GTGGTTTATC AAGGCAAGTA CTCTCACTGC 
ATGAGGATCG GTGTGGCCGT ACCGCTCAAC AGCACTGTGT CCAGACTGAG AGAGGCCGTG 
TCACGCGAGA CCAAGATCCC ACCCGACCAG TTTGTCCTGA CTGAAATGTA TTACGACGGT 
TTCCACCGCT CCTTTTGTGA CGATGACGAC GATCTTGATA TCATTCAAGA AAGTGACTCA 
GTCTTTGCTT TTGAAACCCC TGAGACTTTT AGATTAGAAA ACATTCGCTC TAAAAGAGGG 
AGCCTCCTTG CGAATCTGAA TCAAAATAAT TTGAAATACG GAGCAGAGAG CAGCCGGACG 
CCGTCTTTCA TGCAGGGCGC AGTGAATCCA GCATCGCCCA ACAAAAACAC TGGATCTGAG 
AAAATGATTC TGCTGATCTG TAACAGAGCC TGCACTGGCC ATCAAGGACG GAGGTTCGGC 
CTTCCATTTG TACTATATCT AGAGCGCAGT GTGACGTGGG ACGTGTTACA GAAGGAGATC 
CTGGAGAAAA TGCGTCACCT GACAAGGCCA GGGGTTTACG TTCAGATTGG ACCCTTCAGT 
CTGCGAGTTG TCGGAGTGGT TGGAATCACC TATCTGCTGC CGCAAGAGGA GCAGCCCCTG 
TGTCATCCAA CTGTGGAAAG AGCATATAAG TCCTGTGGGC CAGGAGGACC TCCTCATGTT 
AAGATTGTAG TGGAGTGGGA CAAGGAGACA AAGGAATATT TGTTTGGACA CACTGAGGAA 
GAGTACATCC CTGATGCTGA GAGTGTGTAT CTGCACAGAG AGCAGCACCA TCAGCCGCAG 
GCCTGCACCC TCGCTCAGTG CTTCCAGCTC TACACCAAAG AGGAGCAGTT GGCCCCAGAT 
GATGCCTGGC GTTGCCCACA TTGCAGGCAG CTGCAACAGG GACGGATCAA ACTCAGCCTG 
TGGACCCTGC CGGATGTTCT CATACTGCAC CTCAAGAGGT TTAGACAGGA AGGAGATAGG 
AGGGTTAAGA TGCAGAACAT GGTGAGATTT CCTTTGCTTG GTATGGATAT GGCACCACAC 
GTGGTGAAAA GAAGCCAGAG CAGCTGGAGC TTGCCTTCCC ACTGGTCACC TTGGAGAAGG 
CCTTACGGAC TCGGACGTAA CCCAGACGAC TACCTGTATG ATCTCTATGC TGTGTGTAAC 
CATCATGGCA ATATGCATGG AGGACATTAC ACAGCGTACT GTAAGAATTC CATTGATGGC 
CAGTGGTATT GCTTCGATGA CAGTGAGGTG CAGCCTGTAG CAGATGAAGA CGTCTGTCAG 
CAGACCGCAT ACATTCTGTT CTATCAAAGG AGGACAACTA TTCCCTCATG GTCGGCAAAC 
AGCTCTGTTG CAGGTTCCAC CAGTTCATCT TTATGTGAAC ACTGGGTTAA CCGACTGCCA 
GGCAGCAGGC CACCTAGTTT GGCGTCCGGA GCCTCCTCAA GACGTACATC CCTGATCTCT 
CTGGCAGAGT CGGTGGAGTT TCCCGGTGAC CGCAGTGAAG ACGATGCTTT AGCGCTTACT 
AACACAGGAT CTCCCGCCTT GCTCAGAGGA TTTTCAACAA GGCCCTTCGT GAGAAGCATT 
CAGCGGCAGA GCTTGTCCTC CAGATCATCT GTTACAAGCC CACTGGCTTT CAGCGAAAAT 
GGGATGAAAC CTTCCTGGTC TCTGTCCACT AAACTCCAAA TGAGATCCAA CTCACCCTCG 
CGATTGTCTT TGGACTCTCG CTCTTCACCT CCTCTAGAGA GGATAGGAGA AGCTTGTGAT 
GATAAAGTGT CCACGTCTTG CTTTGGCGGC TACAGCAGAC ATGAGCGTCA GCCTAGCAGT 
AAAGCCCCGT TGGCCATGAT GGAGGGTAAC GGTAGTGACG ATGGTAATGG CAAACGAATC 
CTAGACGAGG CTTACTGCCG AGCTCCACTA CAGGCTGACA AGAAAAGTTC AAAAGGTGAG 
CCGGTTGACA ACAATAACCA GATCAATGCT GTGGACCAGA ACTCTCTCGG ACCGCTCTCC 
AAAGAACAGA AACATAAGAG CTCAGAAAAG AAAACTGAAG GGTCTGGCAG CAAAAAGAGC 
TCTACTAAGA CCAAGGGGGA CCAAGAAAAG CCCTCCAAAA AGCGGCAGAG CACCTCCTCT 
GTTACAAAAT CTCCATCCTC CCAAGTGTCT TCCAGCCCTC AAGTGAAGGG CACCAAAACG 
ACCAATCTAA AAGAAAAGAG CAATTCTCCC AAAAGTACCA AGGGTACACC TTCAGGAACT 
CCCTCCAGAA AGAAAGAGTC TTCTGAGTCT CCAGTGTCAG GAGGCACAAA GGGAAAACCG 
TCCCTTACTT CCACACCCCA GTCCTCTGCC TCTCCCTCAC CTACATCCAA GAAGAGTTCC 
TCGCTGGCTG AGAGGAACTC TGTTTCTGGC AAGAAGAAGC TGGTAGAGAG AGGCTCCAGC 
AAGGATTTGA CACACACCAG TCCCCTTGCG GAGAAGTCTA GAGTCAGTGC CACCCCAGTA 
CGGACATCCC AGCCTAGGAC TGCTGAGGGA AGCCGTCCCG AAAGGAGGAC TGTGAGAAGC 
TCCAGTAGTA GTTCCTCAGT CACCAGCCTG CGTTCCCCAA GTGTCTCCTC GAGAGACCTG 
TGCCGCAGCA GTAAATCTGA GGAAAAGGGA CTCTCATTTT TTAGAAGTGC GCTTCGGCAA 
AAAGAAACCA GACGGTCAGC CGATCTGGGA AAGACCACAA TTCTATCCAA GAAAGCTGCC 
GAGAGGACAG CAAAGTCCAG CAGCCAAAAC AAACTCAATG CCGATGAGGG CGAAGGCTCT 
GGAAACTCTT CCCAGCAAGT CTCTCCGGAG CCTCGCTCCA ACAAGTCTGC CAACAATGAG 
AAAGAGTCCT CGAAGAACTC CACTCCTAGC AAACGCTCAC TCTTACCAGT AGGCAAGTCC 
AAGTCTTCCA ATTCTGAGAC TAGTCTTCTG TCTCCCATCA ATGGGAAGAG GCCTCTTGAA 
AAGGTGGCAT CCTCTAGAAA GCTTTCTTCC AGCATGCAAA CCTCTGCACG GCCAACACAG 
ACGCCTCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164285.1
CDS	1330..5241
Translation	MSSKSSSKEKKSGFSKKLFRRGSVRSVGSFMSRVLKTLSTLSHFGSELHAPDGEKDDGGFTSFKNEGKGSVASDESDCGGFLSRDKIPGVAGLKNHGNTCFMNAILQCLSNTELFAEYLALEQYRGDQTDDEKPKTNGVVGRKGAQDRGEVTEQLSGLVRALWTFEYTPQHSRDFKNAVSRSAMQYRGNAQHDAQEFLLWLLDRVHEDLNHLVHPNIRGSVKPPTEEDDAALEGPSPPLSAGSFVQELFQAQYRSSLTCPHCQKQSNTFDPFLCISLPIPLPHTRPLYVTVVYQGKYSHCMRIGVAVPLNSTVSRLREAVSRETKIPPDQFVLTEMYYDGFHRSFCDDDDDLDIIQESDSVFAFETPETFRLENIRSKRGSLLANLNQNNLKYGAESSRTPSFMQGAVNPASPNKNTGSEKMILLICNRACTGHQGRRFGLPFVLYLERSVTWDVLQKEILEKMRHLTRPGVYVQIGPFSLRVVGVVGITYLLPQEEQPLCHPTVERAYKSCGPGGPPHVKIVVEWDKETKEYLFGHTEEEYIPDAESVYLHREQHHQPQACTLAQCFQLYTKEEQLAPDDAWRCPHCRQLQQGRIKLSLWTLPDVLILHLKRFRQEGDRRVKMQNMVRFPLLGMDMAPHVVKRSQSSWSLPSHWSPWRRPYGLGRNPDDYLYDLYAVCNHHGNMHGGHYTAYCKNSIDGQWYCFDDSEVQPVADEDVCQQTAYILFYQRRTTIPSWSANSSVAGSTSSSLCEHWVNRLPGSRPPSLASGASSRRTSLISLAESVEFPGDRSEDDALALTNTGSPALLRGFSTRPFVRSIQRQSLSSRSSVTSPLAFSENGMKPSWSLSTKLQMRSNSPSRLSLDSRSSPPLERIGEACDDKVSTSCFGGYSRHERQPSSKAPLAMMEGNGSDDGNGKRILDEAYCRAPLQADKKSSKGEPVDNNNQINAVDQNSLGPLSKEQKHKSSEKKTEGSGSKKSSTKTKGDQEKPSKKRQSTSSVTKSPSSQVSSSPQVKGTKTTNLKEKSNSPKSTKGTPSGTPSRKKESSESPVSGGTKGKPSLTSTPQSSASPSPTSKKSSSLAERNSVSGKKKLVERGSSKDLTHTSPLAEKSRVSATPVRTSQPRTAEGSRPERRTVRSSSSSSSVTSLRSPSVSSRDLCRSSKSEEKGLSFFRSALRQKETRRSADLGKTTILSKKAAERTAKSSSQNKLNADEGEGSGNSSQQVSPEPRSNKSANNEKESSKNSTPSKRSLLPVGKSKSSNSETSLLSPINGKRPLEKVASSRKLSSSMQTSARPTQTPQ

Target ORF information:

RefSeq Version	XM_005164228.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164228.3

ORF Insert Sequence:

ATGTCCAGCA AATCTTCCAG CAAAGAGAAG AAGTCCGGCT TCAGCAAGAA GCTGTTCCGC 
CGGGGTTCGG TGCGCTCCGT GGGCAGTTTT ATGAGCAGGG TCCTCAAGAC CCTCTCAACA 
CTCTCTCACT TCGGCTCTGA GCTGCACGCG CCCGATGGAG AAAAGGACGA CGGCGGATTC 
ACATCCTTCA AGAACGAAGG AAAGGGCTCA GTCGCGTCGG ACGAGAGCGA CTGCGGGGGC 
TTTCTGTCCA GGGACAAGAT CCCCGGCGTG GCCGGTTTGA AAAACCACGG CAATACGTGT 
TTCATGAACG CTATTCTGCA GTGCCTGAGC AACACAGAGC TGTTCGCCGA GTATTTGGCC 
CTGGAACAGT ACCGAGGAGA TCAGACTGAC GACGAGAAGC CCAAGACCAA CGGGGTCGTG 
GGCAGAAAGG GCGCTCAGGA CCGGGGAGAG GTGACCGAAC AGCTGTCCGG GCTGGTCCGA 
GCCTTGTGGA CATTTGAGTA CACACCGCAG CACAGTCGAG ACTTCAAAAA TGCTGTTTCT 
AGGAGTGCCA TGCAATATCG GGGAAATGCA CAACACGATG CCCAGGAGTT TCTTCTCTGG 
TTACTGGACC GCGTCCATGA AGACCTCAAC CATCTGGTAC ACCCCAACAT CAGAGGATCT 
GTTAAGCCAC CCACAGAGGA AGACGATGCA GCTTTAGAGG GACCTTCCCC TCCTCTGTCA 
GCCGGCTCAT TTGTCCAGGA GCTGTTTCAA GCTCAGTACA GATCCTCGCT AACCTGTCCA 
CACTGTCAGA AACAGAGCAA CACTTTTGAT CCTTTTCTGT GTATCTCCCT CCCGATTCCA 
TTACCTCACA CACGGCCTCT GTATGTGACT GTGGTTTATC AAGGCAAGTA CTCTCACTGC 
ATGAGGATCG GTGTGGCCGT ACCGCTCAAC AGCACTGTGT CCAGACTGAG AGAGGCCGTG 
TCACGCGAGA CCAAGATCCC ACCCGACCAG TTTGTCCTGA CTGAAATGTA TTACGACGGT 
TTCCACCGCT CCTTTTGTGA CGATGACGAC GATCTTGATA TCATTCAAGA AAGTGACTCA 
GTCTTTGCTT TTGAAACCCC TGAGACTTTT AGATTAGAAA ACATTCGCTC TAAAAGAGGG 
AGCCTCCTTG CGAATCTGAA TCAAAATAAT TTGAAATACG GAGCAGAGAG CAGCCGGACG 
CCGTCTTTCA TGCAGGGCGC AGTGAATCCA GCATCGCCCA ACAAAAACAC TGGATCTGAG 
AAAATGATTC TGCTGATCTG TAACAGAGCC TGCACTGGCC ATCAAGGACG GAGGTTCGGC 
CTTCCATTTG TACTATATCT AGAGCGCAGT GTGACGTGGG ACGTGTTACA GAAGGAGATC 
CTGGAGAAAA TGCGTCACCT GACAAGGCCA GGGGTTTACG TTCAGATTGG ACCCTTCAGT 
CTGCGAGTTG TCGGAGTGGT TGGAATCACC TATCTGCTGC CGCAAGAGGA GCAGCCCCTG 
TGTCATCCAA CTGTGGAAAG AGCATATAAG TCCTGTGGGC CAGGAGGACC TCCTCATGTT 
AAGATTGTAG TGGAGTGGGA CAAGGAGACA AAGGAATATT TGTTTGGACA CACTGAGGAA 
GAGTACATCC CTGATGCTGA GAGTGTGTAT CTGCACAGAG AGCAGCACCA TCAGCCGCAG 
GCCTGCACCC TCGCTCAGTG CTTCCAGCTC TACACCAAAG AGGAGCAGTT GGCCCCAGAT 
GATGCCTGGC GTTGCCCACA TTGCAGGCAG CTGCAACAGG GACGGATCAA ACTCAGCCTG 
TGGACCCTGC CGGATGTTCT CATACTGCAC CTCAAGAGGT TTAGACAGGA AGGAGATAGG 
AGGGTTAAGA TGCAGAACAT GGTGAGATTT CCTTTGCTTG GTATGGATAT GGCACCACAC 
GTGGTGAAAA GAAGCCAGAG CAGCTGGAGC TTGCCTTCCC ACTGGTCACC TTGGAGAAGG 
CCTTACGGAC TCGGACGTAA CCCAGACGAC TACCTGTATG ATCTCTATGC TGTGTGTAAC 
CATCATGGCA ATATGCATGG AGGACATTAC ACAGCGTACT GTAAGAATTC CATTGATGGC 
CAGTGGTATT GCTTCGATGA CAGTGAGGTG CAGCCTGTAG CAGATGAAGA CGTCTGTCAG 
CAGACCGCAT ACATTCTGTT CTATCAAAGG AGGACAACTA TTCCCTCATG GTCGGCAAAC 
AGCTCTGTTG CAGGTTCCAC CAGTTCATCT TTATGTGAAC ACTGGGTTAA CCGACTGCCA 
GGCAGCAGGC CACCTAGTTT GGCGTCCGGA GCCTCCTCAA GACGTACATC CCTGATCTCT 
CTGGCAGAGT CGGTGGAGTT TCCCGGTGAC CGCAGTGAAG ACGATGCTTT AGCGCTTACT 
AACACAGGAT CTCCCGCCTT GCTCAGAGGA TTTTCAACAA GGCCCTTCGT GAGAAGCATT 
CAGCGGCAGA GCTTGTCCTC CAGATCATCT GTTACAAGCC CACTGGCTTT CAGCGAAAAT 
GGGATGAAAC CTTCCTGGTC TCTGTCCACT AAACTCCAAA TGAGATCCAA CTCACCCTCG 
CGATTGTCTT TGGACTCTCG CTCTTCACCT CCTCTAGAGA GGATAGGAGA AGCTTGTGAT 
GATAAAGTGT CCACGTCTTG CTTTGGCGGC TACAGCAGAC ATGAGCGTCA GCCTAGCAGT 
AAAGCCCCGT TGGCCATGAT GGAGGGTAAC GGTAGTGACG ATGGTAATGG CAAACGAATC 
CTAGACGAGG CTTACTGCCG AGCTCCACTA CAGGCTGACA AGAAAAGTTC AAAAGGTGAG 
CCGGTTGACA ACAATAACCA GATCAATGCT GTGGACCAGA ACTCTCTCGG ACCGCTCTCC 
AAAGAACAGA AACATAAGAG CTCAGAAAAG AAAACTGAAG GGTCTGGCAG CAAAAAGAGC 
TCTACTAAGA CCAAGGGGGA CCAAGAAAAG CCCTCCAAAA AGCGGCAGAG CACCTCCTCT 
GTTACAAAAT CTCCATCCTC CCAAGTGTCT TCCAGCCCTC AAGTGAAGGG CACCAAAACG 
ACCAATCTAA AAGAAAAGAG CAATTCTCCC AAAAGTACCA AGGGTACACC TTCAGGAACT 
CCCTCCAGAA AGAAAGAGTC TTCTGAGTCT CCAGTGTCAG GAGGCACAAA GGGAAAACCG 
TCCCTTACTT CCACACCCCA GTCCTCTGCC TCTCCCTCAC CTACATCCAA GAAGAGTTCC 
TCGCTGGCTG AGAGGAACTC TGTTTCTGGC AAGAAGAAGC TGGTAGAGAG AGGCTCCAGC 
AAGGATTTGA CACACACCAG TCCCCTTGCG GAGAAGTCTA GAGTCAGTGC CACCCCAGTA 
CGGACATCCC AGCCTAGGAC TGCTGAGGGA AGCCGTCCCG AAAGGAGGAC TGTGAGAAGC 
TCCAGTAGTA GTTCCTCAGT CACCAGCCTG CGTTCCCCAA GTGTCTCCTC GAGAGACCTG 
TGCCGCAGCA GTAAATCTGA GGAAAAGGGA CTCTCATTTT TTAGAAGTGC GCTTCGGCAA 
AAAGAAACCA GACGGTCAGC CGATCTGGGA AAGACCACAA TTCTATCCAA GAAAGCTGCC 
GAGAGGACAG CAAAGTCCAG CAGCCAAAAC AAACTCAATG CCGATGAGGG CGAAGGCTCT 
GGAAACTCTT CCCAGCAAGT CTCTCCGGAG CCTCGCTCCA ACAAGTCTGC CAACAATGAG 
AAAGAGTCCT CGAAGAACTC CACTCCTAGC AAACGCTCAC TCTTACCAGT AGGCAAGTCC 
AAGTCTTCCA ATTCTGAGAC TAGTCTTCTG TCTCCCATCA ATGGGAAGAG GCCTCTTGAA 
AAGGTGGCAT CCTCTAGAAA GCTTTCTTCC AGCATGCAAA CCTCTGCACG GCCAACACAG 
ACGCCTCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp31 ( XM_005164228.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164285.1 Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08711
Clone ID Related Accession (Same CDS sequence)	XM_005164228.3 , XM_005164228.4
Accession Version	XM_005164228.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 31 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164228.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AATCTTCCAG CAAAGAGAAG AAGTCCGGCT TCAGCAAGAA GCTGTTCCGC 
CGGGGTTCGG TGCGCTCCGT GGGCAGTTTT ATGAGCAGGG TCCTCAAGAC CCTCTCAACA 
CTCTCTCACT TCGGCTCTGA GCTGCACGCG CCCGATGGAG AAAAGGACGA CGGCGGATTC 
ACATCCTTCA AGAACGAAGG AAAGGGCTCA GTCGCGTCGG ACGAGAGCGA CTGCGGGGGC 
TTTCTGTCCA GGGACAAGAT CCCCGGCGTG GCCGGTTTGA AAAACCACGG CAATACGTGT 
TTCATGAACG CTATTCTGCA GTGCCTGAGC AACACAGAGC TGTTCGCCGA GTATTTGGCC 
CTGGAACAGT ACCGAGGAGA TCAGACTGAC GACGAGAAGC CCAAGACCAA CGGGGTCGTG 
GGCAGAAAGG GCGCTCAGGA CCGGGGAGAG GTGACCGAAC AGCTGTCCGG GCTGGTCCGA 
GCCTTGTGGA CATTTGAGTA CACACCGCAG CACAGTCGAG ACTTCAAAAA TGCTGTTTCT 
AGGAGTGCCA TGCAATATCG GGGAAATGCA CAACACGATG CCCAGGAGTT TCTTCTCTGG 
TTACTGGACC GCGTCCATGA AGACCTCAAC CATCTGGTAC ACCCCAACAT CAGAGGATCT 
GTTAAGCCAC CCACAGAGGA AGACGATGCA GCTTTAGAGG GACCTTCCCC TCCTCTGTCA 
GCCGGCTCAT TTGTCCAGGA GCTGTTTCAA GCTCAGTACA GATCCTCGCT AACCTGTCCA 
CACTGTCAGA AACAGAGCAA CACTTTTGAT CCTTTTCTGT GTATCTCCCT CCCGATTCCA 
TTACCTCACA CACGGCCTCT GTATGTGACT GTGGTTTATC AAGGCAAGTA CTCTCACTGC 
ATGAGGATCG GTGTGGCCGT ACCGCTCAAC AGCACTGTGT CCAGACTGAG AGAGGCCGTG 
TCACGCGAGA CCAAGATCCC ACCCGACCAG TTTGTCCTGA CTGAAATGTA TTACGACGGT 
TTCCACCGCT CCTTTTGTGA CGATGACGAC GATCTTGATA TCATTCAAGA AAGTGACTCA 
GTCTTTGCTT TTGAAACCCC TGAGACTTTT AGATTAGAAA ACATTCGCTC TAAAAGAGGG 
AGCCTCCTTG CGAATCTGAA TCAAAATAAT TTGAAATACG GAGCAGAGAG CAGCCGGACG 
CCGTCTTTCA TGCAGGGCGC AGTGAATCCA GCATCGCCCA ACAAAAACAC TGGATCTGAG 
AAAATGATTC TGCTGATCTG TAACAGAGCC TGCACTGGCC ATCAAGGACG GAGGTTCGGC 
CTTCCATTTG TACTATATCT AGAGCGCAGT GTGACGTGGG ACGTGTTACA GAAGGAGATC 
CTGGAGAAAA TGCGTCACCT GACAAGGCCA GGGGTTTACG TTCAGATTGG ACCCTTCAGT 
CTGCGAGTTG TCGGAGTGGT TGGAATCACC TATCTGCTGC CGCAAGAGGA GCAGCCCCTG 
TGTCATCCAA CTGTGGAAAG AGCATATAAG TCCTGTGGGC CAGGAGGACC TCCTCATGTT 
AAGATTGTAG TGGAGTGGGA CAAGGAGACA AAGGAATATT TGTTTGGACA CACTGAGGAA 
GAGTACATCC CTGATGCTGA GAGTGTGTAT CTGCACAGAG AGCAGCACCA TCAGCCGCAG 
GCCTGCACCC TCGCTCAGTG CTTCCAGCTC TACACCAAAG AGGAGCAGTT GGCCCCAGAT 
GATGCCTGGC GTTGCCCACA TTGCAGGCAG CTGCAACAGG GACGGATCAA ACTCAGCCTG 
TGGACCCTGC CGGATGTTCT CATACTGCAC CTCAAGAGGT TTAGACAGGA AGGAGATAGG 
AGGGTTAAGA TGCAGAACAT GGTGAGATTT CCTTTGCTTG GTATGGATAT GGCACCACAC 
GTGGTGAAAA GAAGCCAGAG CAGCTGGAGC TTGCCTTCCC ACTGGTCACC TTGGAGAAGG 
CCTTACGGAC TCGGACGTAA CCCAGACGAC TACCTGTATG ATCTCTATGC TGTGTGTAAC 
CATCATGGCA ATATGCATGG AGGACATTAC ACAGCGTACT GTAAGAATTC CATTGATGGC 
CAGTGGTATT GCTTCGATGA CAGTGAGGTG CAGCCTGTAG CAGATGAAGA CGTCTGTCAG 
CAGACCGCAT ACATTCTGTT CTATCAAAGG AGGACAACTA TTCCCTCATG GTCGGCAAAC 
AGCTCTGTTG CAGGTTCCAC CAGTTCATCT TTATGTGAAC ACTGGGTTAA CCGACTGCCA 
GGCAGCAGGC CACCTAGTTT GGCGTCCGGA GCCTCCTCAA GACGTACATC CCTGATCTCT 
CTGGCAGAGT CGGTGGAGTT TCCCGGTGAC CGCAGTGAAG ACGATGCTTT AGCGCTTACT 
AACACAGGAT CTCCCGCCTT GCTCAGAGGA TTTTCAACAA GGCCCTTCGT GAGAAGCATT 
CAGCGGCAGA GCTTGTCCTC CAGATCATCT GTTACAAGCC CACTGGCTTT CAGCGAAAAT 
GGGATGAAAC CTTCCTGGTC TCTGTCCACT AAACTCCAAA TGAGATCCAA CTCACCCTCG 
CGATTGTCTT TGGACTCTCG CTCTTCACCT CCTCTAGAGA GGATAGGAGA AGCTTGTGAT 
GATAAAGTGT CCACGTCTTG CTTTGGCGGC TACAGCAGAC ATGAGCGTCA GCCTAGCAGT 
AAAGCCCCGT TGGCCATGAT GGAGGGTAAC GGTAGTGACG ATGGTAATGG CAAACGAATC 
CTAGACGAGG CTTACTGCCG AGCTCCACTA CAGGCTGACA AGAAAAGTTC AAAAGGTGAG 
CCGGTTGACA ACAATAACCA GATCAATGCT GTGGACCAGA ACTCTCTCGG ACCGCTCTCC 
AAAGAACAGA AACATAAGAG CTCAGAAAAG AAAACTGAAG GGTCTGGCAG CAAAAAGAGC 
TCTACTAAGA CCAAGGGGGA CCAAGAAAAG CCCTCCAAAA AGCGGCAGAG CACCTCCTCT 
GTTACAAAAT CTCCATCCTC CCAAGTGTCT TCCAGCCCTC AAGTGAAGGG CACCAAAACG 
ACCAATCTAA AAGAAAAGAG CAATTCTCCC AAAAGTACCA AGGGTACACC TTCAGGAACT 
CCCTCCAGAA AGAAAGAGTC TTCTGAGTCT CCAGTGTCAG GAGGCACAAA GGGAAAACCG 
TCCCTTACTT CCACACCCCA GTCCTCTGCC TCTCCCTCAC CTACATCCAA GAAGAGTTCC 
TCGCTGGCTG AGAGGAACTC TGTTTCTGGC AAGAAGAAGC TGGTAGAGAG AGGCTCCAGC 
AAGGATTTGA CACACACCAG TCCCCTTGCG GAGAAGTCTA GAGTCAGTGC CACCCCAGTA 
CGGACATCCC AGCCTAGGAC TGCTGAGGGA AGCCGTCCCG AAAGGAGGAC TGTGAGAAGC 
TCCAGTAGTA GTTCCTCAGT CACCAGCCTG CGTTCCCCAA GTGTCTCCTC GAGAGACCTG 
TGCCGCAGCA GTAAATCTGA GGAAAAGGGA CTCTCATTTT TTAGAAGTGC GCTTCGGCAA 
AAAGAAACCA GACGGTCAGC CGATCTGGGA AAGACCACAA TTCTATCCAA GAAAGCTGCC 
GAGAGGACAG CAAAGTCCAG CAGCCAAAAC AAACTCAATG CCGATGAGGG CGAAGGCTCT 
GGAAACTCTT CCCAGCAAGT CTCTCCGGAG CCTCGCTCCA ACAAGTCTGC CAACAATGAG 
AAAGAGTCCT CGAAGAACTC CACTCCTAGC AAACGCTCAC TCTTACCAGT AGGCAAGTCC 
AAGTCTTCCA ATTCTGAGAC TAGTCTTCTG TCTCCCATCA ATGGGAAGAG GCCTCTTGAA 
AAGGTGGCAT CCTCTAGAAA GCTTTCTTCC AGCATGCAAA CCTCTGCACG GCCAACACAG 
ACGCCTCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164285.1
CDS	1304..5215
Translation	MSSKSSSKEKKSGFSKKLFRRGSVRSVGSFMSRVLKTLSTLSHFGSELHAPDGEKDDGGFTSFKNEGKGSVASDESDCGGFLSRDKIPGVAGLKNHGNTCFMNAILQCLSNTELFAEYLALEQYRGDQTDDEKPKTNGVVGRKGAQDRGEVTEQLSGLVRALWTFEYTPQHSRDFKNAVSRSAMQYRGNAQHDAQEFLLWLLDRVHEDLNHLVHPNIRGSVKPPTEEDDAALEGPSPPLSAGSFVQELFQAQYRSSLTCPHCQKQSNTFDPFLCISLPIPLPHTRPLYVTVVYQGKYSHCMRIGVAVPLNSTVSRLREAVSRETKIPPDQFVLTEMYYDGFHRSFCDDDDDLDIIQESDSVFAFETPETFRLENIRSKRGSLLANLNQNNLKYGAESSRTPSFMQGAVNPASPNKNTGSEKMILLICNRACTGHQGRRFGLPFVLYLERSVTWDVLQKEILEKMRHLTRPGVYVQIGPFSLRVVGVVGITYLLPQEEQPLCHPTVERAYKSCGPGGPPHVKIVVEWDKETKEYLFGHTEEEYIPDAESVYLHREQHHQPQACTLAQCFQLYTKEEQLAPDDAWRCPHCRQLQQGRIKLSLWTLPDVLILHLKRFRQEGDRRVKMQNMVRFPLLGMDMAPHVVKRSQSSWSLPSHWSPWRRPYGLGRNPDDYLYDLYAVCNHHGNMHGGHYTAYCKNSIDGQWYCFDDSEVQPVADEDVCQQTAYILFYQRRTTIPSWSANSSVAGSTSSSLCEHWVNRLPGSRPPSLASGASSRRTSLISLAESVEFPGDRSEDDALALTNTGSPALLRGFSTRPFVRSIQRQSLSSRSSVTSPLAFSENGMKPSWSLSTKLQMRSNSPSRLSLDSRSSPPLERIGEACDDKVSTSCFGGYSRHERQPSSKAPLAMMEGNGSDDGNGKRILDEAYCRAPLQADKKSSKGEPVDNNNQINAVDQNSLGPLSKEQKHKSSEKKTEGSGSKKSSTKTKGDQEKPSKKRQSTSSVTKSPSSQVSSSPQVKGTKTTNLKEKSNSPKSTKGTPSGTPSRKKESSESPVSGGTKGKPSLTSTPQSSASPSPTSKKSSSLAERNSVSGKKKLVERGSSKDLTHTSPLAEKSRVSATPVRTSQPRTAEGSRPERRTVRSSSSSSSVTSLRSPSVSSRDLCRSSKSEEKGLSFFRSALRQKETRRSADLGKTTILSKKAAERTAKSSSQNKLNADEGEGSGNSSQQVSPEPRSNKSANNEKESSKNSTPSKRSLLPVGKSKSSNSETSLLSPINGKRPLEKVASSRKLSSSMQTSARPTQTPQ

Target ORF information:

RefSeq Version	XM_005164228.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164228.4

ORF Insert Sequence:

ATGTCCAGCA AATCTTCCAG CAAAGAGAAG AAGTCCGGCT TCAGCAAGAA GCTGTTCCGC 
CGGGGTTCGG TGCGCTCCGT GGGCAGTTTT ATGAGCAGGG TCCTCAAGAC CCTCTCAACA 
CTCTCTCACT TCGGCTCTGA GCTGCACGCG CCCGATGGAG AAAAGGACGA CGGCGGATTC 
ACATCCTTCA AGAACGAAGG AAAGGGCTCA GTCGCGTCGG ACGAGAGCGA CTGCGGGGGC 
TTTCTGTCCA GGGACAAGAT CCCCGGCGTG GCCGGTTTGA AAAACCACGG CAATACGTGT 
TTCATGAACG CTATTCTGCA GTGCCTGAGC AACACAGAGC TGTTCGCCGA GTATTTGGCC 
CTGGAACAGT ACCGAGGAGA TCAGACTGAC GACGAGAAGC CCAAGACCAA CGGGGTCGTG 
GGCAGAAAGG GCGCTCAGGA CCGGGGAGAG GTGACCGAAC AGCTGTCCGG GCTGGTCCGA 
GCCTTGTGGA CATTTGAGTA CACACCGCAG CACAGTCGAG ACTTCAAAAA TGCTGTTTCT 
AGGAGTGCCA TGCAATATCG GGGAAATGCA CAACACGATG CCCAGGAGTT TCTTCTCTGG 
TTACTGGACC GCGTCCATGA AGACCTCAAC CATCTGGTAC ACCCCAACAT CAGAGGATCT 
GTTAAGCCAC CCACAGAGGA AGACGATGCA GCTTTAGAGG GACCTTCCCC TCCTCTGTCA 
GCCGGCTCAT TTGTCCAGGA GCTGTTTCAA GCTCAGTACA GATCCTCGCT AACCTGTCCA 
CACTGTCAGA AACAGAGCAA CACTTTTGAT CCTTTTCTGT GTATCTCCCT CCCGATTCCA 
TTACCTCACA CACGGCCTCT GTATGTGACT GTGGTTTATC AAGGCAAGTA CTCTCACTGC 
ATGAGGATCG GTGTGGCCGT ACCGCTCAAC AGCACTGTGT CCAGACTGAG AGAGGCCGTG 
TCACGCGAGA CCAAGATCCC ACCCGACCAG TTTGTCCTGA CTGAAATGTA TTACGACGGT 
TTCCACCGCT CCTTTTGTGA CGATGACGAC GATCTTGATA TCATTCAAGA AAGTGACTCA 
GTCTTTGCTT TTGAAACCCC TGAGACTTTT AGATTAGAAA ACATTCGCTC TAAAAGAGGG 
AGCCTCCTTG CGAATCTGAA TCAAAATAAT TTGAAATACG GAGCAGAGAG CAGCCGGACG 
CCGTCTTTCA TGCAGGGCGC AGTGAATCCA GCATCGCCCA ACAAAAACAC TGGATCTGAG 
AAAATGATTC TGCTGATCTG TAACAGAGCC TGCACTGGCC ATCAAGGACG GAGGTTCGGC 
CTTCCATTTG TACTATATCT AGAGCGCAGT GTGACGTGGG ACGTGTTACA GAAGGAGATC 
CTGGAGAAAA TGCGTCACCT GACAAGGCCA GGGGTTTACG TTCAGATTGG ACCCTTCAGT 
CTGCGAGTTG TCGGAGTGGT TGGAATCACC TATCTGCTGC CGCAAGAGGA GCAGCCCCTG 
TGTCATCCAA CTGTGGAAAG AGCATATAAG TCCTGTGGGC CAGGAGGACC TCCTCATGTT 
AAGATTGTAG TGGAGTGGGA CAAGGAGACA AAGGAATATT TGTTTGGACA CACTGAGGAA 
GAGTACATCC CTGATGCTGA GAGTGTGTAT CTGCACAGAG AGCAGCACCA TCAGCCGCAG 
GCCTGCACCC TCGCTCAGTG CTTCCAGCTC TACACCAAAG AGGAGCAGTT GGCCCCAGAT 
GATGCCTGGC GTTGCCCACA TTGCAGGCAG CTGCAACAGG GACGGATCAA ACTCAGCCTG 
TGGACCCTGC CGGATGTTCT CATACTGCAC CTCAAGAGGT TTAGACAGGA AGGAGATAGG 
AGGGTTAAGA TGCAGAACAT GGTGAGATTT CCTTTGCTTG GTATGGATAT GGCACCACAC 
GTGGTGAAAA GAAGCCAGAG CAGCTGGAGC TTGCCTTCCC ACTGGTCACC TTGGAGAAGG 
CCTTACGGAC TCGGACGTAA CCCAGACGAC TACCTGTATG ATCTCTATGC TGTGTGTAAC 
CATCATGGCA ATATGCATGG AGGACATTAC ACAGCGTACT GTAAGAATTC CATTGATGGC 
CAGTGGTATT GCTTCGATGA CAGTGAGGTG CAGCCTGTAG CAGATGAAGA CGTCTGTCAG 
CAGACCGCAT ACATTCTGTT CTATCAAAGG AGGACAACTA TTCCCTCATG GTCGGCAAAC 
AGCTCTGTTG CAGGTTCCAC CAGTTCATCT TTATGTGAAC ACTGGGTTAA CCGACTGCCA 
GGCAGCAGGC CACCTAGTTT GGCGTCCGGA GCCTCCTCAA GACGTACATC CCTGATCTCT 
CTGGCAGAGT CGGTGGAGTT TCCCGGTGAC CGCAGTGAAG ACGATGCTTT AGCGCTTACT 
AACACAGGAT CTCCCGCCTT GCTCAGAGGA TTTTCAACAA GGCCCTTCGT GAGAAGCATT 
CAGCGGCAGA GCTTGTCCTC CAGATCATCT GTTACAAGCC CACTGGCTTT CAGCGAAAAT 
GGGATGAAAC CTTCCTGGTC TCTGTCCACT AAACTCCAAA TGAGATCCAA CTCACCCTCG 
CGATTGTCTT TGGACTCTCG CTCTTCACCT CCTCTAGAGA GGATAGGAGA AGCTTGTGAT 
GATAAAGTGT CCACGTCTTG CTTTGGCGGC TACAGCAGAC ATGAGCGTCA GCCTAGCAGT 
AAAGCCCCGT TGGCCATGAT GGAGGGTAAC GGTAGTGACG ATGGTAATGG CAAACGAATC 
CTAGACGAGG CTTACTGCCG AGCTCCACTA CAGGCTGACA AGAAAAGTTC AAAAGGTGAG 
CCGGTTGACA ACAATAACCA GATCAATGCT GTGGACCAGA ACTCTCTCGG ACCGCTCTCC 
AAAGAACAGA AACATAAGAG CTCAGAAAAG AAAACTGAAG GGTCTGGCAG CAAAAAGAGC 
TCTACTAAGA CCAAGGGGGA CCAAGAAAAG CCCTCCAAAA AGCGGCAGAG CACCTCCTCT 
GTTACAAAAT CTCCATCCTC CCAAGTGTCT TCCAGCCCTC AAGTGAAGGG CACCAAAACG 
ACCAATCTAA AAGAAAAGAG CAATTCTCCC AAAAGTACCA AGGGTACACC TTCAGGAACT 
CCCTCCAGAA AGAAAGAGTC TTCTGAGTCT CCAGTGTCAG GAGGCACAAA GGGAAAACCG 
TCCCTTACTT CCACACCCCA GTCCTCTGCC TCTCCCTCAC CTACATCCAA GAAGAGTTCC 
TCGCTGGCTG AGAGGAACTC TGTTTCTGGC AAGAAGAAGC TGGTAGAGAG AGGCTCCAGC 
AAGGATTTGA CACACACCAG TCCCCTTGCG GAGAAGTCTA GAGTCAGTGC CACCCCAGTA 
CGGACATCCC AGCCTAGGAC TGCTGAGGGA AGCCGTCCCG AAAGGAGGAC TGTGAGAAGC 
TCCAGTAGTA GTTCCTCAGT CACCAGCCTG CGTTCCCCAA GTGTCTCCTC GAGAGACCTG 
TGCCGCAGCA GTAAATCTGA GGAAAAGGGA CTCTCATTTT TTAGAAGTGC GCTTCGGCAA 
AAAGAAACCA GACGGTCAGC CGATCTGGGA AAGACCACAA TTCTATCCAA GAAAGCTGCC 
GAGAGGACAG CAAAGTCCAG CAGCCAAAAC AAACTCAATG CCGATGAGGG CGAAGGCTCT 
GGAAACTCTT CCCAGCAAGT CTCTCCGGAG CCTCGCTCCA ACAAGTCTGC CAACAATGAG 
AAAGAGTCCT CGAAGAACTC CACTCCTAGC AAACGCTCAC TCTTACCAGT AGGCAAGTCC 
AAGTCTTCCA ATTCTGAGAC TAGTCTTCTG TCTCCCATCA ATGGGAAGAG GCCTCTTGAA 
AAGGTGGCAT CCTCTAGAAA GCTTTCTTCC AGCATGCAAA CCTCTGCACG GCCAACACAG 
ACGCCTCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp31 ( XM_005164229.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164286.1 Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X2, mRNA.

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CloneID	ODa08712
Clone ID Related Accession (Same CDS sequence)	XM_005164229.4 , XM_005164229.3
Accession Version	XM_005164229.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 31 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164229.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AATCTTCCAG CAAAGAGAAG AAGTCCGGCT TCAGCAAGAA GCTGTTCCGC 
CGGGGTTCGG TGCGCTCCGT GGGCAGTTTT ATGAGCAGGG TCCTCAAGAC CCTCTCAACA 
CTCTCTCACT TCGGCTCTGA GCTGCACGCG CCCGATGGAG AAAAGGACGA CGGCGGATTC 
ACATCCTTCA AGAACGAAGG AAAGGGCTCA GTCGCGTCGG ACGAGAGCGA CTGCGGGGGC 
TTTCTGTCCA GGGACAAGAT CCCCGGCGTG GCCGGTTTGA AAAACCACGG CAATACGTGT 
TTCATGAACG CTATTCTGCA GTGCCTGAGC AACACAGAGC TGTTCGCCGA GTATTTGGCC 
CTGGAACAGT ACCGAGGAGA TCAGACTGAC GACGAGAAGC CCAAGACCAA CGGGGTCGTG 
GGCAGAAAGG GCGCTCAGGA CCGGGGAGAG GTGACCGAAC AGCTGTCCGG GCTGGTCCGA 
GCCTTGTGGA CATTTGAGTA CACACCGCAG CACAGTCGAG ACTTCAAAAA TGCTGTTTCT 
AGGAGTGCCA TGCAATATCG GGGAAATGCA CAACACGATG CCCAGGAGTT TCTTCTCTGG 
TTACTGGACC GCGTCCATGA AGACCTCAAC CATCTGGTAC ACCCCAACAT CAGAGGATCT 
GTTAAGCCAC CCACAGAGGA AGACGATGCA GCTTTAGAGG GACCTTCCCC TCCTCTGTCA 
GCCGGCTCAT TTGTCCAGGA GCTGTTTCAA GCTCAGTACA GATCCTCGCT AACCTGTCCA 
CACTGTCAGA AACAGAGCAA CACTTTTGAT CCTTTTCTGT GTATCTCCCT CCCGATTCCA 
TTACCTCACA CACGGCCTCT GTATGTGACT GTGGTTTATC AAGGCAAGTA CTCTCACTGC 
ATGAGGATCG GTGTGGCCGT ACCGCTCAAC AGCACTGTGT CCAGACTGAG AGAGGCCGTG 
TCACGCGAGA CCAAGATCCC ACCCGACCAG TTTGTCCTGA CTGAAATGTA TTACGACGGT 
TTCCACCGCT CCTTTTGTGA CGATGACGAC GATCTTGATA TCATTCAAGA AAGTGACTCA 
GTCTTTGCTT TTGAAACCCC TGAGACTTTT AGATTAGAAA ACATTCGCTC TAAAAGAGGG 
AGCCTCCTTG CGAATCTGAA TCAAAATAAT TTGAAATACG GAGCAGAGAG CAGCCGGACG 
CCGTCTTTCA TGCAGGGCGC AGTGAATCCA GCATCGCCCA ACAAAAACAC TGGATCTGAG 
AAAATGATTC TGCTGATCTG TAACAGAGCC TGCACTGGCC ATCAAGGACG GAGGTTCGGC 
CTTCCATTTG TACTATATCT AGAGCGCAGT GTGACGTGGG ACGTGTTACA GAAGGAGATC 
CTGGAGAAAA TGCGTCACCT GACAAGGCCA GGGGTTTACG TTCAGATTGG ACCCTTCAGT 
CTGCGAGTTG TCGGAGTGGT TGGAATCACC TATCTGCTGC CGCAAGAGGA GCAGCCCCTG 
TGTCATCCAA CTGTGGAAAG AGCATATAAG TCCTGTGGGC CAGGAGGACC TCCTCATGTT 
AAGATTGTAG TGGAGTGGGA CAAGGAGACA AAGGAATATT TGTTTGGACA CACTGAGGAA 
GAGTACATCC CTGATGCTGA GAGTGTGTAT CTGCACAGAG AGCAGCACCA TCAGCCGCAG 
GCCTGCACCC TCGCTCAGTG CTTCCAGCTC TACACCAAAG AGGAGCAGTT GGCCCCAGAT 
GATGCCTGGC GTTGCCCACA TTGCAGGCAG CTGCAACAGG GACGGATCAA ACTCAGCCTG 
TGGACCCTGC CGGATGTTCT CATACTGCAC CTCAAGAGGT TTAGACAGGA AGGAGATAGG 
AGGGTTAAGA TGCAGAACAT GGTGAGATTT CCTTTGCTTG GTATGGATAT GGCACCACAC 
GTGGTGAAAA GAAGCCAGAG CAGCTGGAGC TTGCCTTCCC ACTGGTCACC TTGGAGAAGG 
CCTTACGGAC TCGGACGTAA CCCAGACGAC TACCTGTATG ATCTCTATGC TGTGTGTAAC 
CATCATGGCA ATATGCATGG AGGACATTAC ACAGCGTACT GTAAGAATTC CATTGATGGC 
CAGTGGTATT GCTTCGATGA CAGTGAGGTG CAGCCTGTAG CAGATGAAGA CGTCTGTCAG 
CAGACCGCAT ACATTCTGTT CTATCAAAGG AGGACAACTA TTCCCTCATG GTCGGCAAAC 
AGCTCTGTTG CAGGTTCCAC CAGTTCATCT TTATGTGAAC ACTGGGTTAA CCGACTGCCA 
GGCAGCAGGC CACCTAGTTT GGCGTCCGGA GCCTCCTCAA GACGTACATC CCTGATCTCT 
CTGGCAGAGT CGGTGGAGTT TCCCGGTGAC CGCAGTGAAG ACGATGGAGG ATTTTCAACA 
AGGCCCTTCG TGAGAAGCAT TCAGCGGCAG AGCTTGTCCT CCAGATCATC TGTTACAAGC 
CCACTGGCTT TCAGCGAAAA TGGGATGAAA CCTTCCTGGT CTCTGTCCAC TAAACTCCAA 
ATGAGATCCA ACTCACCCTC GCGATTGTCT TTGGACTCTC GCTCTTCACC TCCTCTAGAG 
AGGATAGGAG AAGCTTGTGA TGATAAAGTG TCCACGTCTT GCTTTGGCGG CTACAGCAGA 
CATGAGCGTC AGCCTAGCAG TAAAGCCCCG TTGGCCATGA TGGAGGGTAA CGGTAGTGAC 
GATGGTAATG GCAAACGAAT CCTAGACGAG GCTTACTGCC GAGCTCCACT ACAGGCTGAC 
AAGAAAAGTT CAAAAGGTGA GCCGGTTGAC AACAATAACC AGATCAATGC TGTGGACCAG 
AACTCTCTCG GACCGCTCTC CAAAGAACAG AAACATAAGA GCTCAGAAAA GAAAACTGAA 
GGGTCTGGCA GCAAAAAGAG CTCTACTAAG ACCAAGGGGG ACCAAGAAAA GCCCTCCAAA 
AAGCGGCAGA GCACCTCCTC TGTTACAAAA TCTCCATCCT CCCAAGTGTC TTCCAGCCCT 
CAAGTGAAGG GCACCAAAAC GACCAATCTA AAAGAAAAGA GCAATTCTCC CAAAAGTACC 
AAGGGTACAC CTTCAGGAAC TCCCTCCAGA AAGAAAGAGT CTTCTGAGTC TCCAGTGTCA 
GGAGGCACAA AGGGAAAACC GTCCCTTACT TCCACACCCC AGTCCTCTGC CTCTCCCTCA 
CCTACATCCA AGAAGAGTTC CTCGCTGGCT GAGAGGAACT CTGTTTCTGG CAAGAAGAAG 
CTGGTAGAGA GAGGCTCCAG CAAGGATTTG ACACACACCA GTCCCCTTGC GGAGAAGTCT 
AGAGTCAGTG CCACCCCAGT ACGGACATCC CAGCCTAGGA CTGCTGAGGG AAGCCGTCCC 
GAAAGGAGGA CTGTGAGAAG CTCCAGTAGT AGTTCCTCAG TCACCAGCCT GCGTTCCCCA 
AGTGTCTCCT CGAGAGACCT GTGCCGCAGC AGTAAATCTG AGGAAAAGGG ACTCTCATTT 
TTTAGAAGTG CGCTTCGGCA AAAAGAAACC AGACGGTCAG CCGATCTGGG AAAGACCACA 
ATTCTATCCA AGAAAGCTGC CGAGAGGACA GCAAAGTCCA GCAGCCAAAA CAAACTCAAT 
GCCGATGAGG GCGAAGGCTC TGGAAACTCT TCCCAGCAAG TCTCTCCGGA GCCTCGCTCC 
AACAAGTCTG CCAACAATGA GAAAGAGTCC TCGAAGAACT CCACTCCTAG CAAACGCTCA 
CTCTTACCAG TAGGCAAGTC CAAGTCTTCC AATTCTGAGA CTAGTCTTCT GTCTCCCATC 
AATGGGAAGA GGCCTCTTGA AAAGGTGGCA TCCTCTAGAA AGCTTTCTTC CAGCATGCAA 
ACCTCTGCAC GGCCAACACA GACGCCTCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164286.1
CDS	1303..5175
Translation	MSSKSSSKEKKSGFSKKLFRRGSVRSVGSFMSRVLKTLSTLSHFGSELHAPDGEKDDGGFTSFKNEGKGSVASDESDCGGFLSRDKIPGVAGLKNHGNTCFMNAILQCLSNTELFAEYLALEQYRGDQTDDEKPKTNGVVGRKGAQDRGEVTEQLSGLVRALWTFEYTPQHSRDFKNAVSRSAMQYRGNAQHDAQEFLLWLLDRVHEDLNHLVHPNIRGSVKPPTEEDDAALEGPSPPLSAGSFVQELFQAQYRSSLTCPHCQKQSNTFDPFLCISLPIPLPHTRPLYVTVVYQGKYSHCMRIGVAVPLNSTVSRLREAVSRETKIPPDQFVLTEMYYDGFHRSFCDDDDDLDIIQESDSVFAFETPETFRLENIRSKRGSLLANLNQNNLKYGAESSRTPSFMQGAVNPASPNKNTGSEKMILLICNRACTGHQGRRFGLPFVLYLERSVTWDVLQKEILEKMRHLTRPGVYVQIGPFSLRVVGVVGITYLLPQEEQPLCHPTVERAYKSCGPGGPPHVKIVVEWDKETKEYLFGHTEEEYIPDAESVYLHREQHHQPQACTLAQCFQLYTKEEQLAPDDAWRCPHCRQLQQGRIKLSLWTLPDVLILHLKRFRQEGDRRVKMQNMVRFPLLGMDMAPHVVKRSQSSWSLPSHWSPWRRPYGLGRNPDDYLYDLYAVCNHHGNMHGGHYTAYCKNSIDGQWYCFDDSEVQPVADEDVCQQTAYILFYQRRTTIPSWSANSSVAGSTSSSLCEHWVNRLPGSRPPSLASGASSRRTSLISLAESVEFPGDRSEDDGGFSTRPFVRSIQRQSLSSRSSVTSPLAFSENGMKPSWSLSTKLQMRSNSPSRLSLDSRSSPPLERIGEACDDKVSTSCFGGYSRHERQPSSKAPLAMMEGNGSDDGNGKRILDEAYCRAPLQADKKSSKGEPVDNNNQINAVDQNSLGPLSKEQKHKSSEKKTEGSGSKKSSTKTKGDQEKPSKKRQSTSSVTKSPSSQVSSSPQVKGTKTTNLKEKSNSPKSTKGTPSGTPSRKKESSESPVSGGTKGKPSLTSTPQSSASPSPTSKKSSSLAERNSVSGKKKLVERGSSKDLTHTSPLAEKSRVSATPVRTSQPRTAEGSRPERRTVRSSSSSSSVTSLRSPSVSSRDLCRSSKSEEKGLSFFRSALRQKETRRSADLGKTTILSKKAAERTAKSSSQNKLNADEGEGSGNSSQQVSPEPRSNKSANNEKESSKNSTPSKRSLLPVGKSKSSNSETSLLSPINGKRPLEKVASSRKLSSSMQTSARPTQTPQ

Target ORF information:

RefSeq Version	XM_005164229.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164229.4

ORF Insert Sequence:

ATGTCCAGCA AATCTTCCAG CAAAGAGAAG AAGTCCGGCT TCAGCAAGAA GCTGTTCCGC 
CGGGGTTCGG TGCGCTCCGT GGGCAGTTTT ATGAGCAGGG TCCTCAAGAC CCTCTCAACA 
CTCTCTCACT TCGGCTCTGA GCTGCACGCG CCCGATGGAG AAAAGGACGA CGGCGGATTC 
ACATCCTTCA AGAACGAAGG AAAGGGCTCA GTCGCGTCGG ACGAGAGCGA CTGCGGGGGC 
TTTCTGTCCA GGGACAAGAT CCCCGGCGTG GCCGGTTTGA AAAACCACGG CAATACGTGT 
TTCATGAACG CTATTCTGCA GTGCCTGAGC AACACAGAGC TGTTCGCCGA GTATTTGGCC 
CTGGAACAGT ACCGAGGAGA TCAGACTGAC GACGAGAAGC CCAAGACCAA CGGGGTCGTG 
GGCAGAAAGG GCGCTCAGGA CCGGGGAGAG GTGACCGAAC AGCTGTCCGG GCTGGTCCGA 
GCCTTGTGGA CATTTGAGTA CACACCGCAG CACAGTCGAG ACTTCAAAAA TGCTGTTTCT 
AGGAGTGCCA TGCAATATCG GGGAAATGCA CAACACGATG CCCAGGAGTT TCTTCTCTGG 
TTACTGGACC GCGTCCATGA AGACCTCAAC CATCTGGTAC ACCCCAACAT CAGAGGATCT 
GTTAAGCCAC CCACAGAGGA AGACGATGCA GCTTTAGAGG GACCTTCCCC TCCTCTGTCA 
GCCGGCTCAT TTGTCCAGGA GCTGTTTCAA GCTCAGTACA GATCCTCGCT AACCTGTCCA 
CACTGTCAGA AACAGAGCAA CACTTTTGAT CCTTTTCTGT GTATCTCCCT CCCGATTCCA 
TTACCTCACA CACGGCCTCT GTATGTGACT GTGGTTTATC AAGGCAAGTA CTCTCACTGC 
ATGAGGATCG GTGTGGCCGT ACCGCTCAAC AGCACTGTGT CCAGACTGAG AGAGGCCGTG 
TCACGCGAGA CCAAGATCCC ACCCGACCAG TTTGTCCTGA CTGAAATGTA TTACGACGGT 
TTCCACCGCT CCTTTTGTGA CGATGACGAC GATCTTGATA TCATTCAAGA AAGTGACTCA 
GTCTTTGCTT TTGAAACCCC TGAGACTTTT AGATTAGAAA ACATTCGCTC TAAAAGAGGG 
AGCCTCCTTG CGAATCTGAA TCAAAATAAT TTGAAATACG GAGCAGAGAG CAGCCGGACG 
CCGTCTTTCA TGCAGGGCGC AGTGAATCCA GCATCGCCCA ACAAAAACAC TGGATCTGAG 
AAAATGATTC TGCTGATCTG TAACAGAGCC TGCACTGGCC ATCAAGGACG GAGGTTCGGC 
CTTCCATTTG TACTATATCT AGAGCGCAGT GTGACGTGGG ACGTGTTACA GAAGGAGATC 
CTGGAGAAAA TGCGTCACCT GACAAGGCCA GGGGTTTACG TTCAGATTGG ACCCTTCAGT 
CTGCGAGTTG TCGGAGTGGT TGGAATCACC TATCTGCTGC CGCAAGAGGA GCAGCCCCTG 
TGTCATCCAA CTGTGGAAAG AGCATATAAG TCCTGTGGGC CAGGAGGACC TCCTCATGTT 
AAGATTGTAG TGGAGTGGGA CAAGGAGACA AAGGAATATT TGTTTGGACA CACTGAGGAA 
GAGTACATCC CTGATGCTGA GAGTGTGTAT CTGCACAGAG AGCAGCACCA TCAGCCGCAG 
GCCTGCACCC TCGCTCAGTG CTTCCAGCTC TACACCAAAG AGGAGCAGTT GGCCCCAGAT 
GATGCCTGGC GTTGCCCACA TTGCAGGCAG CTGCAACAGG GACGGATCAA ACTCAGCCTG 
TGGACCCTGC CGGATGTTCT CATACTGCAC CTCAAGAGGT TTAGACAGGA AGGAGATAGG 
AGGGTTAAGA TGCAGAACAT GGTGAGATTT CCTTTGCTTG GTATGGATAT GGCACCACAC 
GTGGTGAAAA GAAGCCAGAG CAGCTGGAGC TTGCCTTCCC ACTGGTCACC TTGGAGAAGG 
CCTTACGGAC TCGGACGTAA CCCAGACGAC TACCTGTATG ATCTCTATGC TGTGTGTAAC 
CATCATGGCA ATATGCATGG AGGACATTAC ACAGCGTACT GTAAGAATTC CATTGATGGC 
CAGTGGTATT GCTTCGATGA CAGTGAGGTG CAGCCTGTAG CAGATGAAGA CGTCTGTCAG 
CAGACCGCAT ACATTCTGTT CTATCAAAGG AGGACAACTA TTCCCTCATG GTCGGCAAAC 
AGCTCTGTTG CAGGTTCCAC CAGTTCATCT TTATGTGAAC ACTGGGTTAA CCGACTGCCA 
GGCAGCAGGC CACCTAGTTT GGCGTCCGGA GCCTCCTCAA GACGTACATC CCTGATCTCT 
CTGGCAGAGT CGGTGGAGTT TCCCGGTGAC CGCAGTGAAG ACGATGGAGG ATTTTCAACA 
AGGCCCTTCG TGAGAAGCAT TCAGCGGCAG AGCTTGTCCT CCAGATCATC TGTTACAAGC 
CCACTGGCTT TCAGCGAAAA TGGGATGAAA CCTTCCTGGT CTCTGTCCAC TAAACTCCAA 
ATGAGATCCA ACTCACCCTC GCGATTGTCT TTGGACTCTC GCTCTTCACC TCCTCTAGAG 
AGGATAGGAG AAGCTTGTGA TGATAAAGTG TCCACGTCTT GCTTTGGCGG CTACAGCAGA 
CATGAGCGTC AGCCTAGCAG TAAAGCCCCG TTGGCCATGA TGGAGGGTAA CGGTAGTGAC 
GATGGTAATG GCAAACGAAT CCTAGACGAG GCTTACTGCC GAGCTCCACT ACAGGCTGAC 
AAGAAAAGTT CAAAAGGTGA GCCGGTTGAC AACAATAACC AGATCAATGC TGTGGACCAG 
AACTCTCTCG GACCGCTCTC CAAAGAACAG AAACATAAGA GCTCAGAAAA GAAAACTGAA 
GGGTCTGGCA GCAAAAAGAG CTCTACTAAG ACCAAGGGGG ACCAAGAAAA GCCCTCCAAA 
AAGCGGCAGA GCACCTCCTC TGTTACAAAA TCTCCATCCT CCCAAGTGTC TTCCAGCCCT 
CAAGTGAAGG GCACCAAAAC GACCAATCTA AAAGAAAAGA GCAATTCTCC CAAAAGTACC 
AAGGGTACAC CTTCAGGAAC TCCCTCCAGA AAGAAAGAGT CTTCTGAGTC TCCAGTGTCA 
GGAGGCACAA AGGGAAAACC GTCCCTTACT TCCACACCCC AGTCCTCTGC CTCTCCCTCA 
CCTACATCCA AGAAGAGTTC CTCGCTGGCT GAGAGGAACT CTGTTTCTGG CAAGAAGAAG 
CTGGTAGAGA GAGGCTCCAG CAAGGATTTG ACACACACCA GTCCCCTTGC GGAGAAGTCT 
AGAGTCAGTG CCACCCCAGT ACGGACATCC CAGCCTAGGA CTGCTGAGGG AAGCCGTCCC 
GAAAGGAGGA CTGTGAGAAG CTCCAGTAGT AGTTCCTCAG TCACCAGCCT GCGTTCCCCA 
AGTGTCTCCT CGAGAGACCT GTGCCGCAGC AGTAAATCTG AGGAAAAGGG ACTCTCATTT 
TTTAGAAGTG CGCTTCGGCA AAAAGAAACC AGACGGTCAG CCGATCTGGG AAAGACCACA 
ATTCTATCCA AGAAAGCTGC CGAGAGGACA GCAAAGTCCA GCAGCCAAAA CAAACTCAAT 
GCCGATGAGG GCGAAGGCTC TGGAAACTCT TCCCAGCAAG TCTCTCCGGA GCCTCGCTCC 
AACAAGTCTG CCAACAATGA GAAAGAGTCC TCGAAGAACT CCACTCCTAG CAAACGCTCA 
CTCTTACCAG TAGGCAAGTC CAAGTCTTCC AATTCTGAGA CTAGTCTTCT GTCTCCCATC 
AATGGGAAGA GGCCTCTTGA AAAGGTGGCA TCCTCTAGAA AGCTTTCTTC CAGCATGCAA 
ACCTCTGCAC GGCCAACACA GACGCCTCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp31 ( XM_005164229.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164286.1 Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X2, mRNA.

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CloneID	ODa08712
Clone ID Related Accession (Same CDS sequence)	XM_005164229.4 , XM_005164229.3
Accession Version	XM_005164229.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 31 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGCA AATCTTCCAG CAAAGAGAAG AAGTCCGGCT TCAGCAAGAA GCTGTTCCGC 
CGGGGTTCGG TGCGCTCCGT GGGCAGTTTT ATGAGCAGGG TCCTCAAGAC CCTCTCAACA 
CTCTCTCACT TCGGCTCTGA GCTGCACGCG CCCGATGGAG AAAAGGACGA CGGCGGATTC 
ACATCCTTCA AGAACGAAGG AAAGGGCTCA GTCGCGTCGG ACGAGAGCGA CTGCGGGGGC 
TTTCTGTCCA GGGACAAGAT CCCCGGCGTG GCCGGTTTGA AAAACCACGG CAATACGTGT 
TTCATGAACG CTATTCTGCA GTGCCTGAGC AACACAGAGC TGTTCGCCGA GTATTTGGCC 
CTGGAACAGT ACCGAGGAGA TCAGACTGAC GACGAGAAGC CCAAGACCAA CGGGGTCGTG 
GGCAGAAAGG GCGCTCAGGA CCGGGGAGAG GTGACCGAAC AGCTGTCCGG GCTGGTCCGA 
GCCTTGTGGA CATTTGAGTA CACACCGCAG CACAGTCGAG ACTTCAAAAA TGCTGTTTCT 
AGGAGTGCCA TGCAATATCG GGGAAATGCA CAACACGATG CCCAGGAGTT TCTTCTCTGG 
TTACTGGACC GCGTCCATGA AGACCTCAAC CATCTGGTAC ACCCCAACAT CAGAGGATCT 
GTTAAGCCAC CCACAGAGGA AGACGATGCA GCTTTAGAGG GACCTTCCCC TCCTCTGTCA 
GCCGGCTCAT TTGTCCAGGA GCTGTTTCAA GCTCAGTACA GATCCTCGCT AACCTGTCCA 
CACTGTCAGA AACAGAGCAA CACTTTTGAT CCTTTTCTGT GTATCTCCCT CCCGATTCCA 
TTACCTCACA CACGGCCTCT GTATGTGACT GTGGTTTATC AAGGCAAGTA CTCTCACTGC 
ATGAGGATCG GTGTGGCCGT ACCGCTCAAC AGCACTGTGT CCAGACTGAG AGAGGCCGTG 
TCACGCGAGA CCAAGATCCC ACCCGACCAG TTTGTCCTGA CTGAAATGTA TTACGACGGT 
TTCCACCGCT CCTTTTGTGA CGATGACGAC GATCTTGATA TCATTCAAGA AAGTGACTCA 
GTCTTTGCTT TTGAAACCCC TGAGACTTTT AGATTAGAAA ACATTCGCTC TAAAAGAGGG 
AGCCTCCTTG CGAATCTGAA TCAAAATAAT TTGAAATACG GAGCAGAGAG CAGCCGGACG 
CCGTCTTTCA TGCAGGGCGC AGTGAATCCA GCATCGCCCA ACAAAAACAC TGGATCTGAG 
AAAATGATTC TGCTGATCTG TAACAGAGCC TGCACTGGCC ATCAAGGACG GAGGTTCGGC 
CTTCCATTTG TACTATATCT AGAGCGCAGT GTGACGTGGG ACGTGTTACA GAAGGAGATC 
CTGGAGAAAA TGCGTCACCT GACAAGGCCA GGGGTTTACG TTCAGATTGG ACCCTTCAGT 
CTGCGAGTTG TCGGAGTGGT TGGAATCACC TATCTGCTGC CGCAAGAGGA GCAGCCCCTG 
TGTCATCCAA CTGTGGAAAG AGCATATAAG TCCTGTGGGC CAGGAGGACC TCCTCATGTT 
AAGATTGTAG TGGAGTGGGA CAAGGAGACA AAGGAATATT TGTTTGGACA CACTGAGGAA 
GAGTACATCC CTGATGCTGA GAGTGTGTAT CTGCACAGAG AGCAGCACCA TCAGCCGCAG 
GCCTGCACCC TCGCTCAGTG CTTCCAGCTC TACACCAAAG AGGAGCAGTT GGCCCCAGAT 
GATGCCTGGC GTTGCCCACA TTGCAGGCAG CTGCAACAGG GACGGATCAA ACTCAGCCTG 
TGGACCCTGC CGGATGTTCT CATACTGCAC CTCAAGAGGT TTAGACAGGA AGGAGATAGG 
AGGGTTAAGA TGCAGAACAT GGTGAGATTT CCTTTGCTTG GTATGGATAT GGCACCACAC 
GTGGTGAAAA GAAGCCAGAG CAGCTGGAGC TTGCCTTCCC ACTGGTCACC TTGGAGAAGG 
CCTTACGGAC TCGGACGTAA CCCAGACGAC TACCTGTATG ATCTCTATGC TGTGTGTAAC 
CATCATGGCA ATATGCATGG AGGACATTAC ACAGCGTACT GTAAGAATTC CATTGATGGC 
CAGTGGTATT GCTTCGATGA CAGTGAGGTG CAGCCTGTAG CAGATGAAGA CGTCTGTCAG 
CAGACCGCAT ACATTCTGTT CTATCAAAGG AGGACAACTA TTCCCTCATG GTCGGCAAAC 
AGCTCTGTTG CAGGTTCCAC CAGTTCATCT TTATGTGAAC ACTGGGTTAA CCGACTGCCA 
GGCAGCAGGC CACCTAGTTT GGCGTCCGGA GCCTCCTCAA GACGTACATC CCTGATCTCT 
CTGGCAGAGT CGGTGGAGTT TCCCGGTGAC CGCAGTGAAG ACGATGGAGG ATTTTCAACA 
AGGCCCTTCG TGAGAAGCAT TCAGCGGCAG AGCTTGTCCT CCAGATCATC TGTTACAAGC 
CCACTGGCTT TCAGCGAAAA TGGGATGAAA CCTTCCTGGT CTCTGTCCAC TAAACTCCAA 
ATGAGATCCA ACTCACCCTC GCGATTGTCT TTGGACTCTC GCTCTTCACC TCCTCTAGAG 
AGGATAGGAG AAGCTTGTGA TGATAAAGTG TCCACGTCTT GCTTTGGCGG CTACAGCAGA 
CATGAGCGTC AGCCTAGCAG TAAAGCCCCG TTGGCCATGA TGGAGGGTAA CGGTAGTGAC 
GATGGTAATG GCAAACGAAT CCTAGACGAG GCTTACTGCC GAGCTCCACT ACAGGCTGAC 
AAGAAAAGTT CAAAAGGTGA GCCGGTTGAC AACAATAACC AGATCAATGC TGTGGACCAG 
AACTCTCTCG GACCGCTCTC CAAAGAACAG AAACATAAGA GCTCAGAAAA GAAAACTGAA 
GGGTCTGGCA GCAAAAAGAG CTCTACTAAG ACCAAGGGGG ACCAAGAAAA GCCCTCCAAA 
AAGCGGCAGA GCACCTCCTC TGTTACAAAA TCTCCATCCT CCCAAGTGTC TTCCAGCCCT 
CAAGTGAAGG GCACCAAAAC GACCAATCTA AAAGAAAAGA GCAATTCTCC CAAAAGTACC 
AAGGGTACAC CTTCAGGAAC TCCCTCCAGA AAGAAAGAGT CTTCTGAGTC TCCAGTGTCA 
GGAGGCACAA AGGGAAAACC GTCCCTTACT TCCACACCCC AGTCCTCTGC CTCTCCCTCA 
CCTACATCCA AGAAGAGTTC CTCGCTGGCT GAGAGGAACT CTGTTTCTGG CAAGAAGAAG 
CTGGTAGAGA GAGGCTCCAG CAAGGATTTG ACACACACCA GTCCCCTTGC GGAGAAGTCT 
AGAGTCAGTG CCACCCCAGT ACGGACATCC CAGCCTAGGA CTGCTGAGGG AAGCCGTCCC 
GAAAGGAGGA CTGTGAGAAG CTCCAGTAGT AGTTCCTCAG TCACCAGCCT GCGTTCCCCA 
AGTGTCTCCT CGAGAGACCT GTGCCGCAGC AGTAAATCTG AGGAAAAGGG ACTCTCATTT 
TTTAGAAGTG CGCTTCGGCA AAAAGAAACC AGACGGTCAG CCGATCTGGG AAAGACCACA 
ATTCTATCCA AGAAAGCTGC CGAGAGGACA GCAAAGTCCA GCAGCCAAAA CAAACTCAAT 
GCCGATGAGG GCGAAGGCTC TGGAAACTCT TCCCAGCAAG TCTCTCCGGA GCCTCGCTCC 
AACAAGTCTG CCAACAATGA GAAAGAGTCC TCGAAGAACT CCACTCCTAG CAAACGCTCA 
CTCTTACCAG TAGGCAAGTC CAAGTCTTCC AATTCTGAGA CTAGTCTTCT GTCTCCCATC 
AATGGGAAGA GGCCTCTTGA AAAGGTGGCA TCCTCTAGAA AGCTTTCTTC CAGCATGCAA 
ACCTCTGCAC GGCCAACACA GACGCCTCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164286.1
CDS	1329..5201
Translation	MSSKSSSKEKKSGFSKKLFRRGSVRSVGSFMSRVLKTLSTLSHFGSELHAPDGEKDDGGFTSFKNEGKGSVASDESDCGGFLSRDKIPGVAGLKNHGNTCFMNAILQCLSNTELFAEYLALEQYRGDQTDDEKPKTNGVVGRKGAQDRGEVTEQLSGLVRALWTFEYTPQHSRDFKNAVSRSAMQYRGNAQHDAQEFLLWLLDRVHEDLNHLVHPNIRGSVKPPTEEDDAALEGPSPPLSAGSFVQELFQAQYRSSLTCPHCQKQSNTFDPFLCISLPIPLPHTRPLYVTVVYQGKYSHCMRIGVAVPLNSTVSRLREAVSRETKIPPDQFVLTEMYYDGFHRSFCDDDDDLDIIQESDSVFAFETPETFRLENIRSKRGSLLANLNQNNLKYGAESSRTPSFMQGAVNPASPNKNTGSEKMILLICNRACTGHQGRRFGLPFVLYLERSVTWDVLQKEILEKMRHLTRPGVYVQIGPFSLRVVGVVGITYLLPQEEQPLCHPTVERAYKSCGPGGPPHVKIVVEWDKETKEYLFGHTEEEYIPDAESVYLHREQHHQPQACTLAQCFQLYTKEEQLAPDDAWRCPHCRQLQQGRIKLSLWTLPDVLILHLKRFRQEGDRRVKMQNMVRFPLLGMDMAPHVVKRSQSSWSLPSHWSPWRRPYGLGRNPDDYLYDLYAVCNHHGNMHGGHYTAYCKNSIDGQWYCFDDSEVQPVADEDVCQQTAYILFYQRRTTIPSWSANSSVAGSTSSSLCEHWVNRLPGSRPPSLASGASSRRTSLISLAESVEFPGDRSEDDGGFSTRPFVRSIQRQSLSSRSSVTSPLAFSENGMKPSWSLSTKLQMRSNSPSRLSLDSRSSPPLERIGEACDDKVSTSCFGGYSRHERQPSSKAPLAMMEGNGSDDGNGKRILDEAYCRAPLQADKKSSKGEPVDNNNQINAVDQNSLGPLSKEQKHKSSEKKTEGSGSKKSSTKTKGDQEKPSKKRQSTSSVTKSPSSQVSSSPQVKGTKTTNLKEKSNSPKSTKGTPSGTPSRKKESSESPVSGGTKGKPSLTSTPQSSASPSPTSKKSSSLAERNSVSGKKKLVERGSSKDLTHTSPLAEKSRVSATPVRTSQPRTAEGSRPERRTVRSSSSSSSVTSLRSPSVSSRDLCRSSKSEEKGLSFFRSALRQKETRRSADLGKTTILSKKAAERTAKSSSQNKLNADEGEGSGNSSQQVSPEPRSNKSANNEKESSKNSTPSKRSLLPVGKSKSSNSETSLLSPINGKRPLEKVASSRKLSSSMQTSARPTQTPQ

Target ORF information:

RefSeq Version	XM_005164229.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 31 (usp31), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164229.3

ORF Insert Sequence:

ATGTCCAGCA AATCTTCCAG CAAAGAGAAG AAGTCCGGCT TCAGCAAGAA GCTGTTCCGC 
CGGGGTTCGG TGCGCTCCGT GGGCAGTTTT ATGAGCAGGG TCCTCAAGAC CCTCTCAACA 
CTCTCTCACT TCGGCTCTGA GCTGCACGCG CCCGATGGAG AAAAGGACGA CGGCGGATTC 
ACATCCTTCA AGAACGAAGG AAAGGGCTCA GTCGCGTCGG ACGAGAGCGA CTGCGGGGGC 
TTTCTGTCCA GGGACAAGAT CCCCGGCGTG GCCGGTTTGA AAAACCACGG CAATACGTGT 
TTCATGAACG CTATTCTGCA GTGCCTGAGC AACACAGAGC TGTTCGCCGA GTATTTGGCC 
CTGGAACAGT ACCGAGGAGA TCAGACTGAC GACGAGAAGC CCAAGACCAA CGGGGTCGTG 
GGCAGAAAGG GCGCTCAGGA CCGGGGAGAG GTGACCGAAC AGCTGTCCGG GCTGGTCCGA 
GCCTTGTGGA CATTTGAGTA CACACCGCAG CACAGTCGAG ACTTCAAAAA TGCTGTTTCT 
AGGAGTGCCA TGCAATATCG GGGAAATGCA CAACACGATG CCCAGGAGTT TCTTCTCTGG 
TTACTGGACC GCGTCCATGA AGACCTCAAC CATCTGGTAC ACCCCAACAT CAGAGGATCT 
GTTAAGCCAC CCACAGAGGA AGACGATGCA GCTTTAGAGG GACCTTCCCC TCCTCTGTCA 
GCCGGCTCAT TTGTCCAGGA GCTGTTTCAA GCTCAGTACA GATCCTCGCT AACCTGTCCA 
CACTGTCAGA AACAGAGCAA CACTTTTGAT CCTTTTCTGT GTATCTCCCT CCCGATTCCA 
TTACCTCACA CACGGCCTCT GTATGTGACT GTGGTTTATC AAGGCAAGTA CTCTCACTGC 
ATGAGGATCG GTGTGGCCGT ACCGCTCAAC AGCACTGTGT CCAGACTGAG AGAGGCCGTG 
TCACGCGAGA CCAAGATCCC ACCCGACCAG TTTGTCCTGA CTGAAATGTA TTACGACGGT 
TTCCACCGCT CCTTTTGTGA CGATGACGAC GATCTTGATA TCATTCAAGA AAGTGACTCA 
GTCTTTGCTT TTGAAACCCC TGAGACTTTT AGATTAGAAA ACATTCGCTC TAAAAGAGGG 
AGCCTCCTTG CGAATCTGAA TCAAAATAAT TTGAAATACG GAGCAGAGAG CAGCCGGACG 
CCGTCTTTCA TGCAGGGCGC AGTGAATCCA GCATCGCCCA ACAAAAACAC TGGATCTGAG 
AAAATGATTC TGCTGATCTG TAACAGAGCC TGCACTGGCC ATCAAGGACG GAGGTTCGGC 
CTTCCATTTG TACTATATCT AGAGCGCAGT GTGACGTGGG ACGTGTTACA GAAGGAGATC 
CTGGAGAAAA TGCGTCACCT GACAAGGCCA GGGGTTTACG TTCAGATTGG ACCCTTCAGT 
CTGCGAGTTG TCGGAGTGGT TGGAATCACC TATCTGCTGC CGCAAGAGGA GCAGCCCCTG 
TGTCATCCAA CTGTGGAAAG AGCATATAAG TCCTGTGGGC CAGGAGGACC TCCTCATGTT 
AAGATTGTAG TGGAGTGGGA CAAGGAGACA AAGGAATATT TGTTTGGACA CACTGAGGAA 
GAGTACATCC CTGATGCTGA GAGTGTGTAT CTGCACAGAG AGCAGCACCA TCAGCCGCAG 
GCCTGCACCC TCGCTCAGTG CTTCCAGCTC TACACCAAAG AGGAGCAGTT GGCCCCAGAT 
GATGCCTGGC GTTGCCCACA TTGCAGGCAG CTGCAACAGG GACGGATCAA ACTCAGCCTG 
TGGACCCTGC CGGATGTTCT CATACTGCAC CTCAAGAGGT TTAGACAGGA AGGAGATAGG 
AGGGTTAAGA TGCAGAACAT GGTGAGATTT CCTTTGCTTG GTATGGATAT GGCACCACAC 
GTGGTGAAAA GAAGCCAGAG CAGCTGGAGC TTGCCTTCCC ACTGGTCACC TTGGAGAAGG 
CCTTACGGAC TCGGACGTAA CCCAGACGAC TACCTGTATG ATCTCTATGC TGTGTGTAAC 
CATCATGGCA ATATGCATGG AGGACATTAC ACAGCGTACT GTAAGAATTC CATTGATGGC 
CAGTGGTATT GCTTCGATGA CAGTGAGGTG CAGCCTGTAG CAGATGAAGA CGTCTGTCAG 
CAGACCGCAT ACATTCTGTT CTATCAAAGG AGGACAACTA TTCCCTCATG GTCGGCAAAC 
AGCTCTGTTG CAGGTTCCAC CAGTTCATCT TTATGTGAAC ACTGGGTTAA CCGACTGCCA 
GGCAGCAGGC CACCTAGTTT GGCGTCCGGA GCCTCCTCAA GACGTACATC CCTGATCTCT 
CTGGCAGAGT CGGTGGAGTT TCCCGGTGAC CGCAGTGAAG ACGATGGAGG ATTTTCAACA 
AGGCCCTTCG TGAGAAGCAT TCAGCGGCAG AGCTTGTCCT CCAGATCATC TGTTACAAGC 
CCACTGGCTT TCAGCGAAAA TGGGATGAAA CCTTCCTGGT CTCTGTCCAC TAAACTCCAA 
ATGAGATCCA ACTCACCCTC GCGATTGTCT TTGGACTCTC GCTCTTCACC TCCTCTAGAG 
AGGATAGGAG AAGCTTGTGA TGATAAAGTG TCCACGTCTT GCTTTGGCGG CTACAGCAGA 
CATGAGCGTC AGCCTAGCAG TAAAGCCCCG TTGGCCATGA TGGAGGGTAA CGGTAGTGAC 
GATGGTAATG GCAAACGAAT CCTAGACGAG GCTTACTGCC GAGCTCCACT ACAGGCTGAC 
AAGAAAAGTT CAAAAGGTGA GCCGGTTGAC AACAATAACC AGATCAATGC TGTGGACCAG 
AACTCTCTCG GACCGCTCTC CAAAGAACAG AAACATAAGA GCTCAGAAAA GAAAACTGAA 
GGGTCTGGCA GCAAAAAGAG CTCTACTAAG ACCAAGGGGG ACCAAGAAAA GCCCTCCAAA 
AAGCGGCAGA GCACCTCCTC TGTTACAAAA TCTCCATCCT CCCAAGTGTC TTCCAGCCCT 
CAAGTGAAGG GCACCAAAAC GACCAATCTA AAAGAAAAGA GCAATTCTCC CAAAAGTACC 
AAGGGTACAC CTTCAGGAAC TCCCTCCAGA AAGAAAGAGT CTTCTGAGTC TCCAGTGTCA 
GGAGGCACAA AGGGAAAACC GTCCCTTACT TCCACACCCC AGTCCTCTGC CTCTCCCTCA 
CCTACATCCA AGAAGAGTTC CTCGCTGGCT GAGAGGAACT CTGTTTCTGG CAAGAAGAAG 
CTGGTAGAGA GAGGCTCCAG CAAGGATTTG ACACACACCA GTCCCCTTGC GGAGAAGTCT 
AGAGTCAGTG CCACCCCAGT ACGGACATCC CAGCCTAGGA CTGCTGAGGG AAGCCGTCCC 
GAAAGGAGGA CTGTGAGAAG CTCCAGTAGT AGTTCCTCAG TCACCAGCCT GCGTTCCCCA 
AGTGTCTCCT CGAGAGACCT GTGCCGCAGC AGTAAATCTG AGGAAAAGGG ACTCTCATTT 
TTTAGAAGTG CGCTTCGGCA AAAAGAAACC AGACGGTCAG CCGATCTGGG AAAGACCACA 
ATTCTATCCA AGAAAGCTGC CGAGAGGACA GCAAAGTCCA GCAGCCAAAA CAAACTCAAT 
GCCGATGAGG GCGAAGGCTC TGGAAACTCT TCCCAGCAAG TCTCTCCGGA GCCTCGCTCC 
AACAAGTCTG CCAACAATGA GAAAGAGTCC TCGAAGAACT CCACTCCTAG CAAACGCTCA 
CTCTTACCAG TAGGCAAGTC CAAGTCTTCC AATTCTGAGA CTAGTCTTCT GTCTCCCATC 
AATGGGAAGA GGCCTCTTGA AAAGGTGGCA TCCTCTAGAA AGCTTTCTTC CAGCATGCAA 
ACCTCTGCAC GGCCAACACA GACGCCTCAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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usp31 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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