CACNA2D4A cDNA ORF clone, Danio rerio(Zebrafish)

  • Gene

  • Clones

  • gRNAs

The following CACNA2D4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA2D4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa45371 XM_021475260.1
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa58571 XM_017355309.1
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa58572 XM_017355310.2
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa45370 XM_009300430.2
Latest version!
Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa58572 XM_017355310.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa45371
    Clone ID Related Accession (Same CDS sequence) XM_021475260.1
    Accession Version XM_021475260.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTACC CAATACAGCT
    GAGCTTTTTA ACACACAGGC CGAGAGTTTG CTGTTGATGA CCTCTGTAGC CATGCCTGTG
    TTCAGCAAGA AAGAAGAAAC ACTCTCTCAT GGCATCCTCC TCGGTGTGGT TGGCACTGAT
    GTTCCCTTGA AGGAGCTCAT GAGATTGGCT CCTCGATACA AGCTGGGGGT TCACGGCTAT
    GCATTCTTAA ACACTAATAA TGGTTATATA CTCTCTCATC CTGACCTTCG ACCTCTGTAT
    AAAGATGGCA AGAAGCTGAA GCCAAAGCCG AACTATAACA GTGTGGATTT ATCAGAGGTG
    GAGTGGGAGG ATACAGAAGA TGCACTGAGA ACGGCGATGG TAAAAGGGGA AACTGGGACA
    ATCTCTCTGG ACGTCAGAGC AACAGTAGAA AAAGGAAAAA GAGTTGTGTT CCTGAAAAAT
    GAATATTTTT ATACTACCAT CAATGAGACG CCATTTAGTC TTGGGATTGT ACTTACCAAC
    GGTCACGGGA AGAATGTTTT CATCGGAAAT GTTTCAGTTG AAGAAGGCCT ACATGACCTG
    ACTGCGTCTG ATGTCAGTAT TGCTCAAGAA TGGACATACT GTGAGACAGA CATCGATCCA
    CATCATCGCA AATTGACGCA GCTTCAGGCC GTGGTTCGGT ATTTAACAGG AAAAGAACCA
    GATCTGGAAT GTGATGAAAG GCTTCTGCAG CAGACTCTGT TTGACGCAGT GGTCACAGCT
    CCTATGGAAG CCTATTGGAC TGCACTGCTG CTCAACGCCT CCGGTATTGA TGGGGGAGTG
    GAGACAGCGT TCCTGGGCAC GCGCTCTGGC CTCATGAGGA TGATCAGATA TGCTGGCGTA
    GAGAAGCGTG TGGCGAAAAA ATTCTTAACT GCATCAGATA AGGAGAATAT TTTTACTCTA
    GACCATTTCC CTGTTTGGTA TCGGCGTGCG GCAGAAAATC CAGCTGGCCG CTTTTTGTAC
    TACGTTCCTA CAGAGGACAA CTCAGGAGTG AACGGCAGGT TTGTGATTGC TGTCACGCCT
    GTTACAGTCT CTGTGAGCAA AAAGACAGCA GTTGCTGGAG CCATCGGTGT ACAGATGTCT
    CTTGAGATGT TGGAAAAAAG GTTTTGGTCC ATAGCCAACC AGCCGAACGA CACAGATTGT
    TCAGGCATAG AGGGGCTTTG TCCTTTAAGC TGTAGTGATA ATCCGGACAT TAGCTGCTAC
    GTCATTGACA GTAATGGCTT CATCATCATC TCTAGGGACA AGGCTGATGT AGGCAAGTTT
    CTGGGGAAAA TCGAAGGGTC AGTGATGTCC CAACTCTTGA AAATGGGCAT GTTCAAAAGA
    GTTGTACTCT ATGACTATCA GGCCATGTGT AAGGCGCACG CTCACTCTGC TAGTGGCGCT
    CGACCCCTGC TAAGTCCCTT TTATAATCTT TTGGCAGCAC TGAAATGGTT CCTATCCAAC
    TTCCTGTTCC TTTTATTGGA CTTCAACATC TACAATCTGT GGCATTCGGA TCACTTTACA
    GAGGCCAAGA CCTCATTCCA TTCATCTAGT AAGAAGTTAA AGGGTGGCTT TCTGCAGCCG
    TGTGACACTG AATATCCCAG CTTCGTCTAC GAGCCATCAA TCAAAGAAAC CAACAGCCTG
    ATCAAGTGTG GGAGATGTCA AAAGGTGTTC GTAATTCAAC AGATACCAGA TAGCAACTTG
    CTGCTGGTGG TGATTCAGGC TGATTGTGAC TGTTCTCGGC AATATCCACC CATCACCATG
    GAGCCCAAAG AGGTCAAATA CAATGCCTCA GTGAAATGTG ACCGCATGAG ATCTCAGAAG
    ATACGAAGAA GGCCTGAGTC TTGTCACGCT TACCACCCTC AAGAAAATGC CAAGGACTGT
    GGTGGGGCTG TGGCAATCAG CCTGTCTATC ATGCTATTCT TTGCCTGTTT ATCAGTGTCA
    GCACTCATCC ATCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330935.1
    CDS909..4226
    Translation

    Target ORF information:

    RefSeq Version XM_021475260.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475260.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTACC CAATACAGCT
    GAGCTTTTTA ACACACAGGC CGAGAGTTTG CTGTTGATGA CCTCTGTAGC CATGCCTGTG
    TTCAGCAAGA AAGAAGAAAC ACTCTCTCAT GGCATCCTCC TCGGTGTGGT TGGCACTGAT
    GTTCCCTTGA AGGAGCTCAT GAGATTGGCT CCTCGATACA AGCTGGGGGT TCACGGCTAT
    GCATTCTTAA ACACTAATAA TGGTTATATA CTCTCTCATC CTGACCTTCG ACCTCTGTAT
    AAAGATGGCA AGAAGCTGAA GCCAAAGCCG AACTATAACA GTGTGGATTT ATCAGAGGTG
    GAGTGGGAGG ATACAGAAGA TGCACTGAGA ACGGCGATGG TAAAAGGGGA AACTGGGACA
    ATCTCTCTGG ACGTCAGAGC AACAGTAGAA AAAGGAAAAA GAGTTGTGTT CCTGAAAAAT
    GAATATTTTT ATACTACCAT CAATGAGACG CCATTTAGTC TTGGGATTGT ACTTACCAAC
    GGTCACGGGA AGAATGTTTT CATCGGAAAT GTTTCAGTTG AAGAAGGCCT ACATGACCTG
    ACTGCGTCTG ATGTCAGTAT TGCTCAAGAA TGGACATACT GTGAGACAGA CATCGATCCA
    CATCATCGCA AATTGACGCA GCTTCAGGCC GTGGTTCGGT ATTTAACAGG AAAAGAACCA
    GATCTGGAAT GTGATGAAAG GCTTCTGCAG CAGACTCTGT TTGACGCAGT GGTCACAGCT
    CCTATGGAAG CCTATTGGAC TGCACTGCTG CTCAACGCCT CCGGTATTGA TGGGGGAGTG
    GAGACAGCGT TCCTGGGCAC GCGCTCTGGC CTCATGAGGA TGATCAGATA TGCTGGCGTA
    GAGAAGCGTG TGGCGAAAAA ATTCTTAACT GCATCAGATA AGGAGAATAT TTTTACTCTA
    GACCATTTCC CTGTTTGGTA TCGGCGTGCG GCAGAAAATC CAGCTGGCCG CTTTTTGTAC
    TACGTTCCTA CAGAGGACAA CTCAGGAGTG AACGGCAGGT TTGTGATTGC TGTCACGCCT
    GTTACAGTCT CTGTGAGCAA AAAGACAGCA GTTGCTGGAG CCATCGGTGT ACAGATGTCT
    CTTGAGATGT TGGAAAAAAG GTTTTGGTCC ATAGCCAACC AGCCGAACGA CACAGATTGT
    TCAGGCATAG AGGGGCTTTG TCCTTTAAGC TGTAGTGATA ATCCGGACAT TAGCTGCTAC
    GTCATTGACA GTAATGGCTT CATCATCATC TCTAGGGACA AGGCTGATGT AGGCAAGTTT
    CTGGGGAAAA TCGAAGGGTC AGTGATGTCC CAACTCTTGA AAATGGGCAT GTTCAAAAGA
    GTTGTACTCT ATGACTATCA GGCCATGTGT AAGGCGCACG CTCACTCTGC TAGTGGCGCT
    CGACCCCTGC TAAGTCCCTT TTATAATCTT TTGGCAGCAC TGAAATGGTT CCTATCCAAC
    TTCCTGTTCC TTTTATTGGA CTTCAACATC TACAATCTGT GGCATTCGGA TCACTTTACA
    GAGGCCAAGA CCTCATTCCA TTCATCTAGT AAGAAGTTAA AGGGTGGCTT TCTGCAGCCG
    TGTGACACTG AATATCCCAG CTTCGTCTAC GAGCCATCAA TCAAAGAAAC CAACAGCCTG
    ATCAAGTGTG GGAGATGTCA AAAGGTGTTC GTAATTCAAC AGATACCAGA TAGCAACTTG
    CTGCTGGTGG TGATTCAGGC TGATTGTGAC TGTTCTCGGC AATATCCACC CATCACCATG
    GAGCCCAAAG AGGTCAAATA CAATGCCTCA GTGAAATGTG ACCGCATGAG ATCTCAGAAG
    ATACGAAGAA GGCCTGAGTC TTGTCACGCT TACCACCCTC AAGAAAATGC CAAGGACTGT
    GGTGGGGCTG TGGCAATCAG CCTGTCTATC ATGCTATTCT TTGCCTGTTT ATCAGTGTCA
    GCACTCATCC ATCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58571
    Clone ID Related Accession (Same CDS sequence) XM_017355309.1
    Accession Version XM_017355309.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG
    GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA
    ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC
    ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT
    AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG
    AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA
    GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA
    GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC
    ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT
    TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT
    ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG
    CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA
    AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG
    ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC
    ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA
    AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG
    TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC
    AACTCAGGAG GTAGCCTGTA CCCTGCAGTC CACATCAAAA CAACTGAGAC TTTACAGCCT
    TTTGAGAGTA CAGTGCTCAG TATTTTTTTC GTTTCTTTCC CTGCAGTGAA CGGCAGGTTT
    GTGATTGCTG TCACGCCTGT TACAGTCTCT GTGAGCAAAA AGACAGCAGT TGCTGGAGCC
    ATCGGTGTAC AGATGTCTCT TGAGATGTTG GAAAAAAGGT TTTGGTCCAT AGCCAACCAG
    CCGAACGACA CAGATTGTTC AGGCATAGAG GGGCTTTGTC CTTTAAGCTG TAGTGATAAT
    CCGGACATTA GCTGCTACGT CATTGACAGT AATGGCTTCA TCATCATCTC TAGGGACAAG
    GCTGATGTAG GCAAGTTTCT GGGGAAAATC GAAGGGTCAG TGATGTCCCA ACTCTTGAAA
    ATGGGCATGT TCAAAAGAGT TGTACTCTAT GACTATCAGG CCATGTGTAA GGCGCACGCT
    CACTCTGCTA GTGGCGCTCG ACCCCTGCTA AGTCCCTTTT ATAATCTTTT GGCAGCACTG
    AAATGGTTCC TATCCAACTT CCTGTTCCTT TTATTGGACT TCAACATCTA CAATCTGTGG
    CATTCGGATC ACTTTACAGA GGCTAGTAAG AAGTTAAAGG GTGGCTTTCT GCAGCCGTGT
    GACACTGAAT ATCCCAGCTT CGTCTACGAG CCATCAATCA AAGAAACCAA CAGCCTGATC
    AAGTGTGGGA GATGTCAAAA GGTGTTCGTA ATTCAACAGA TACCAGATAG CAACTTGCTG
    CTGGTGGTGA TTCAGGCTGA TTGTGACTGT TCTCGGCAAT ATCCACCCAT CACCATGGAG
    CCCAAAGAGG TCAAATACAA TGCCTCAGTG AAATGTGACC GCATGAGATC TCAGAAGATA
    CGAAGAAGGC CTGAGTCTTG TCACGCTTAC CACCCTCAAG AAAATGCCAA GGACTGTGGT
    GGGGCTGTGG CAATCAGCCT GTCTATCATG CTATTCTTTG CCTGTTTATC AGTGTCAGCA
    CTCATCCATC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210798.1
    CDS441..3815
    Translation

    Target ORF information:

    RefSeq Version XM_017355309.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355309.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG
    GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA
    ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC
    ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT
    AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG
    AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA
    GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA
    GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC
    ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT
    TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT
    ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG
    CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA
    AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG
    ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC
    ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA
    AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG
    TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC
    AACTCAGGAG GTAGCCTGTA CCCTGCAGTC CACATCAAAA CAACTGAGAC TTTACAGCCT
    TTTGAGAGTA CAGTGCTCAG TATTTTTTTC GTTTCTTTCC CTGCAGTGAA CGGCAGGTTT
    GTGATTGCTG TCACGCCTGT TACAGTCTCT GTGAGCAAAA AGACAGCAGT TGCTGGAGCC
    ATCGGTGTAC AGATGTCTCT TGAGATGTTG GAAAAAAGGT TTTGGTCCAT AGCCAACCAG
    CCGAACGACA CAGATTGTTC AGGCATAGAG GGGCTTTGTC CTTTAAGCTG TAGTGATAAT
    CCGGACATTA GCTGCTACGT CATTGACAGT AATGGCTTCA TCATCATCTC TAGGGACAAG
    GCTGATGTAG GCAAGTTTCT GGGGAAAATC GAAGGGTCAG TGATGTCCCA ACTCTTGAAA
    ATGGGCATGT TCAAAAGAGT TGTACTCTAT GACTATCAGG CCATGTGTAA GGCGCACGCT
    CACTCTGCTA GTGGCGCTCG ACCCCTGCTA AGTCCCTTTT ATAATCTTTT GGCAGCACTG
    AAATGGTTCC TATCCAACTT CCTGTTCCTT TTATTGGACT TCAACATCTA CAATCTGTGG
    CATTCGGATC ACTTTACAGA GGCTAGTAAG AAGTTAAAGG GTGGCTTTCT GCAGCCGTGT
    GACACTGAAT ATCCCAGCTT CGTCTACGAG CCATCAATCA AAGAAACCAA CAGCCTGATC
    AAGTGTGGGA GATGTCAAAA GGTGTTCGTA ATTCAACAGA TACCAGATAG CAACTTGCTG
    CTGGTGGTGA TTCAGGCTGA TTGTGACTGT TCTCGGCAAT ATCCACCCAT CACCATGGAG
    CCCAAAGAGG TCAAATACAA TGCCTCAGTG AAATGTGACC GCATGAGATC TCAGAAGATA
    CGAAGAAGGC CTGAGTCTTG TCACGCTTAC CACCCTCAAG AAAATGCCAA GGACTGTGGT
    GGGGCTGTGG CAATCAGCCT GTCTATCATG CTATTCTTTG CCTGTTTATC AGTGTCAGCA
    CTCATCCATC GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58572
    Clone ID Related Accession (Same CDS sequence) XM_017355310.1 , XM_017355310.2
    Accession Version XM_017355310.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG
    GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA
    ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC
    ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT
    AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG
    AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA
    GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA
    GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC
    ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT
    TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT
    ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG
    CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA
    AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG
    ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC
    ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA
    AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG
    TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC
    AACTCAGGAG TGAACGGCAG GTTTGTGATT GCTGTCACGC CTGTTACAGT CTCTGTGAGC
    AAAAAGACAG CAGTTGCTGG AGCCATCGGT GTACAGATGT CTCTTGAGAT GTTGGAAAAA
    AGGTTTTGGT CCATAGCCAA CCAGCCGAAC GACACAGATT GTTCAGGCAT AGAGGGGCTT
    TGTCCTTTAA GCTGTAGTGA TAATCCGGAC ATTAGCTGCT ACGTCATTGA CAGTAATGGC
    TTCATCATCA TCTCTAGGGA CAAGGCTGAT GTAGGCAAGT TTCTGGGGAA AATCGAAGGG
    TCAGTGATGT CCCAACTCTT GAAAATGGGC ATGTTCAAAA GAGTTGTACT CTATGACTAT
    CAGGCCATGT GTAAGGCGCA CGCTCACTCT GCTAGTGGCG CTCGACCCCT GCTAAGTCCC
    TTTTATAATC TTTTGGCAGC ACTGAAATGG TTCCTATCCA ACTTCCTGTT CCTTTTATTG
    GACTTCAACA TCTACAATCT GTGGCATTCG GATCACTTTA CAGAGGCCAA GACCTCATTC
    CATTCATCTA GTAAGAAGTT AAAGGGTGGC TTTCTGCAGC CGTGTGACAC TGAATATCCC
    AGCTTCGTCT ACGAGCCATC AATCAAAGAA ACCAACAGCC TGATCAAGTG TGGGAGATGT
    CAAAAGGTGT TCGTAATTCA ACAGATACCA GATAGCAACT TGCTGCTGGT GGTGATTCAG
    GCTGATTGTG ACTGTTCTCG GCAATATCCA CCCATCACCA TGGAGCCCAA AGAGGTCAAA
    TACAATGCCT CAGTGAAATG TGACCGCATG AGATCTCAGA AGATACGAAG AAGGCCTGAG
    TCTTGTCACG CTTACCACCC TCAAGAAAAT GCCAAGGACT GTGGTGGGGC TGTGGCAATC
    AGCCTGTCTA TCATGCTATT CTTTGCCTGT TTATCAGTGT CAGCACTCAT CCATCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210799.1
    CDS441..3740
    Translation

    Target ORF information:

    RefSeq Version XM_017355310.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG
    GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA
    ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC
    ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT
    AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG
    AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA
    GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA
    GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC
    ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT
    TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT
    ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG
    CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA
    AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG
    ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC
    ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA
    AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG
    TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC
    AACTCAGGAG TGAACGGCAG GTTTGTGATT GCTGTCACGC CTGTTACAGT CTCTGTGAGC
    AAAAAGACAG CAGTTGCTGG AGCCATCGGT GTACAGATGT CTCTTGAGAT GTTGGAAAAA
    AGGTTTTGGT CCATAGCCAA CCAGCCGAAC GACACAGATT GTTCAGGCAT AGAGGGGCTT
    TGTCCTTTAA GCTGTAGTGA TAATCCGGAC ATTAGCTGCT ACGTCATTGA CAGTAATGGC
    TTCATCATCA TCTCTAGGGA CAAGGCTGAT GTAGGCAAGT TTCTGGGGAA AATCGAAGGG
    TCAGTGATGT CCCAACTCTT GAAAATGGGC ATGTTCAAAA GAGTTGTACT CTATGACTAT
    CAGGCCATGT GTAAGGCGCA CGCTCACTCT GCTAGTGGCG CTCGACCCCT GCTAAGTCCC
    TTTTATAATC TTTTGGCAGC ACTGAAATGG TTCCTATCCA ACTTCCTGTT CCTTTTATTG
    GACTTCAACA TCTACAATCT GTGGCATTCG GATCACTTTA CAGAGGCCAA GACCTCATTC
    CATTCATCTA GTAAGAAGTT AAAGGGTGGC TTTCTGCAGC CGTGTGACAC TGAATATCCC
    AGCTTCGTCT ACGAGCCATC AATCAAAGAA ACCAACAGCC TGATCAAGTG TGGGAGATGT
    CAAAAGGTGT TCGTAATTCA ACAGATACCA GATAGCAACT TGCTGCTGGT GGTGATTCAG
    GCTGATTGTG ACTGTTCTCG GCAATATCCA CCCATCACCA TGGAGCCCAA AGAGGTCAAA
    TACAATGCCT CAGTGAAATG TGACCGCATG AGATCTCAGA AGATACGAAG AAGGCCTGAG
    TCTTGTCACG CTTACCACCC TCAAGAAAAT GCCAAGGACT GTGGTGGGGC TGTGGCAATC
    AGCCTGTCTA TCATGCTATT CTTTGCCTGT TTATCAGTGT CAGCACTCAT CCATCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58572
    Clone ID Related Accession (Same CDS sequence) XM_017355310.1 , XM_017355310.2
    Accession Version XM_017355310.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG
    GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA
    ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC
    ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT
    AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG
    AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA
    GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA
    GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC
    ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT
    TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT
    ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG
    CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA
    AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG
    ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC
    ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA
    AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG
    TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC
    AACTCAGGAG TGAACGGCAG GTTTGTGATT GCTGTCACGC CTGTTACAGT CTCTGTGAGC
    AAAAAGACAG CAGTTGCTGG AGCCATCGGT GTACAGATGT CTCTTGAGAT GTTGGAAAAA
    AGGTTTTGGT CCATAGCCAA CCAGCCGAAC GACACAGATT GTTCAGGCAT AGAGGGGCTT
    TGTCCTTTAA GCTGTAGTGA TAATCCGGAC ATTAGCTGCT ACGTCATTGA CAGTAATGGC
    TTCATCATCA TCTCTAGGGA CAAGGCTGAT GTAGGCAAGT TTCTGGGGAA AATCGAAGGG
    TCAGTGATGT CCCAACTCTT GAAAATGGGC ATGTTCAAAA GAGTTGTACT CTATGACTAT
    CAGGCCATGT GTAAGGCGCA CGCTCACTCT GCTAGTGGCG CTCGACCCCT GCTAAGTCCC
    TTTTATAATC TTTTGGCAGC ACTGAAATGG TTCCTATCCA ACTTCCTGTT CCTTTTATTG
    GACTTCAACA TCTACAATCT GTGGCATTCG GATCACTTTA CAGAGGCCAA GACCTCATTC
    CATTCATCTA GTAAGAAGTT AAAGGGTGGC TTTCTGCAGC CGTGTGACAC TGAATATCCC
    AGCTTCGTCT ACGAGCCATC AATCAAAGAA ACCAACAGCC TGATCAAGTG TGGGAGATGT
    CAAAAGGTGT TCGTAATTCA ACAGATACCA GATAGCAACT TGCTGCTGGT GGTGATTCAG
    GCTGATTGTG ACTGTTCTCG GCAATATCCA CCCATCACCA TGGAGCCCAA AGAGGTCAAA
    TACAATGCCT CAGTGAAATG TGACCGCATG AGATCTCAGA AGATACGAAG AAGGCCTGAG
    TCTTGTCACG CTTACCACCC TCAAGAAAAT GCCAAGGACT GTGGTGGGGC TGTGGCAATC
    AGCCTGTCTA TCATGCTATT CTTTGCCTGT TTATCAGTGT CAGCACTCAT CCATCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210799.1
    CDS909..4208
    Translation

    Target ORF information:

    RefSeq Version XM_017355310.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017355310.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG
    GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA
    ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC
    ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT
    AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG
    AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA
    GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA
    GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC
    ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT
    TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT
    ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG
    CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA
    AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG
    ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC
    ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA
    AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG
    TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC
    AACTCAGGAG TGAACGGCAG GTTTGTGATT GCTGTCACGC CTGTTACAGT CTCTGTGAGC
    AAAAAGACAG CAGTTGCTGG AGCCATCGGT GTACAGATGT CTCTTGAGAT GTTGGAAAAA
    AGGTTTTGGT CCATAGCCAA CCAGCCGAAC GACACAGATT GTTCAGGCAT AGAGGGGCTT
    TGTCCTTTAA GCTGTAGTGA TAATCCGGAC ATTAGCTGCT ACGTCATTGA CAGTAATGGC
    TTCATCATCA TCTCTAGGGA CAAGGCTGAT GTAGGCAAGT TTCTGGGGAA AATCGAAGGG
    TCAGTGATGT CCCAACTCTT GAAAATGGGC ATGTTCAAAA GAGTTGTACT CTATGACTAT
    CAGGCCATGT GTAAGGCGCA CGCTCACTCT GCTAGTGGCG CTCGACCCCT GCTAAGTCCC
    TTTTATAATC TTTTGGCAGC ACTGAAATGG TTCCTATCCA ACTTCCTGTT CCTTTTATTG
    GACTTCAACA TCTACAATCT GTGGCATTCG GATCACTTTA CAGAGGCCAA GACCTCATTC
    CATTCATCTA GTAAGAAGTT AAAGGGTGGC TTTCTGCAGC CGTGTGACAC TGAATATCCC
    AGCTTCGTCT ACGAGCCATC AATCAAAGAA ACCAACAGCC TGATCAAGTG TGGGAGATGT
    CAAAAGGTGT TCGTAATTCA ACAGATACCA GATAGCAACT TGCTGCTGGT GGTGATTCAG
    GCTGATTGTG ACTGTTCTCG GCAATATCCA CCCATCACCA TGGAGCCCAA AGAGGTCAAA
    TACAATGCCT CAGTGAAATG TGACCGCATG AGATCTCAGA AGATACGAAG AAGGCCTGAG
    TCTTGTCACG CTTACCACCC TCAAGAAAAT GCCAAGGACT GTGGTGGGGC TGTGGCAATC
    AGCCTGTCTA TCATGCTATT CTTTGCCTGT TTATCAGTGT CAGCACTCAT CCATCGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45370
    Clone ID Related Accession (Same CDS sequence) XM_009300430.2
    Accession Version XM_009300430.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG
    GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA
    ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC
    ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT
    AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG
    AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA
    GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA
    GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC
    ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT
    TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT
    ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG
    CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA
    AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG
    ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC
    ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA
    AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG
    TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC
    AACTCAGGAG GTAGCCTGTA CCCTGCAGTC CACATCAAAA CAACTGAGAC TTTACAGCCT
    TTTGAGAGTA CAGTGCTCAG TATTTTTTTC GTTTCTTTCC CTGCAGTGAA CGGCAGGTTT
    GTGATTGCTG TCACGCCTGT TACAGTCTCT GTGAGCAAAA AGACAGCAGT TGCTGGAGCC
    ATCGGTGTAC AGATGTCTCT TGAGATGTTG GAAAAAAGGT TTTGGTCCAT AGCCAACCAG
    CCGAACGACA CAGATTGTTC AGGCATAGAG GGGCTTTGTC CTTTAAGCTG TAGTGATAAT
    CCGGACATTA GCTGCTACGT CATTGACAGT AATGGCTTCA TCATCATCTC TAGGGACAAG
    GCTGATGTAG GCAAGTTTCT GGGGAAAATC GAAGGGTCAG TGATGTCCCA ACTCTTGAAA
    ATGGGCATGT TCAAAAGAGT TGTACTCTAT GACTATCAGG CCATGTGTAA GGCGCACGCT
    CACTCTGCTA GTGGCGCTCG ACCCCTGCTA AGTCCCTTTT ATAATCTTTT GGCAGCACTG
    AAATGGTTCC TATCCAACTT CCTGTTCCTT TTATTGGACT TCAACATCTA CAATCTGTGG
    CATTCGGATC ACTTTACAGA GGCCAAGACC TCATTCCATT CATCTAGTAA GAAGTTAAAG
    GGTGGCTTTC TGCAGCCGTG TGACACTGAA TATCCCAGCT TCGTCTACGA GCCATCAATC
    AAAGAAACCA ACAGCCTGAT CAAGTGTGGG AGATGTCAAA AGGTGTTCGT AATTCAACAG
    ATACCAGATA GCAACTTGCT GCTGGTGGTG ATTCAGGCTG ATTGTGACTG TTCTCGGCAA
    TATCCACCCA TCACCATGGA GCCCAAAGAG GTCAAATACA ATGCCTCAGT GAAATGTGAC
    CGCATGAGAT CTCAGAAGAT ACGAAGAAGG CCTGAGTCTT GTCACGCTTA CCACCCTCAA
    GAAAATGCCA AGGACTGTGG TGGGGCTGTG GCAATCAGCC TGTCTATCAT GCTATTCTTT
    GCCTGTTTAT CAGTGTCAGC ACTCATCCAT CGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298705.1
    CDS441..3836
    Translation

    Target ORF information:

    RefSeq Version XM_009300430.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300430.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
    AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG
    GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC
    TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA
    AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA
    GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA
    TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC
    ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC
    AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC
    CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG
    GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA
    GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC
    TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG
    AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA
    CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG
    CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT
    TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC
    TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC
    ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG
    CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC
    AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG
    GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC
    CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG
    GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA
    ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC
    ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT
    AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG
    AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA
    GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA
    GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC
    ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT
    TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT
    ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG
    CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA
    AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG
    ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC
    ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA
    AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG
    TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC
    AACTCAGGAG GTAGCCTGTA CCCTGCAGTC CACATCAAAA CAACTGAGAC TTTACAGCCT
    TTTGAGAGTA CAGTGCTCAG TATTTTTTTC GTTTCTTTCC CTGCAGTGAA CGGCAGGTTT
    GTGATTGCTG TCACGCCTGT TACAGTCTCT GTGAGCAAAA AGACAGCAGT TGCTGGAGCC
    ATCGGTGTAC AGATGTCTCT TGAGATGTTG GAAAAAAGGT TTTGGTCCAT AGCCAACCAG
    CCGAACGACA CAGATTGTTC AGGCATAGAG GGGCTTTGTC CTTTAAGCTG TAGTGATAAT
    CCGGACATTA GCTGCTACGT CATTGACAGT AATGGCTTCA TCATCATCTC TAGGGACAAG
    GCTGATGTAG GCAAGTTTCT GGGGAAAATC GAAGGGTCAG TGATGTCCCA ACTCTTGAAA
    ATGGGCATGT TCAAAAGAGT TGTACTCTAT GACTATCAGG CCATGTGTAA GGCGCACGCT
    CACTCTGCTA GTGGCGCTCG ACCCCTGCTA AGTCCCTTTT ATAATCTTTT GGCAGCACTG
    AAATGGTTCC TATCCAACTT CCTGTTCCTT TTATTGGACT TCAACATCTA CAATCTGTGG
    CATTCGGATC ACTTTACAGA GGCCAAGACC TCATTCCATT CATCTAGTAA GAAGTTAAAG
    GGTGGCTTTC TGCAGCCGTG TGACACTGAA TATCCCAGCT TCGTCTACGA GCCATCAATC
    AAAGAAACCA ACAGCCTGAT CAAGTGTGGG AGATGTCAAA AGGTGTTCGT AATTCAACAG
    ATACCAGATA GCAACTTGCT GCTGGTGGTG ATTCAGGCTG ATTGTGACTG TTCTCGGCAA
    TATCCACCCA TCACCATGGA GCCCAAAGAG GTCAAATACA ATGCCTCAGT GAAATGTGAC
    CGCATGAGAT CTCAGAAGAT ACGAAGAAGG CCTGAGTCTT GTCACGCTTA CCACCCTCAA
    GAAAATGCCA AGGACTGTGG TGGGGCTGTG GCAATCAGCC TGTCTATCAT GCTATTCTTT
    GCCTGTTTAT CAGTGTCAGC ACTCATCCAT CGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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