slka cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slka
Entrez Gene ID	568838
Full Name	STE20-like kinase a
Synonyms	im:7150762,si:ch211-223p8.7,slkn,wu:fk52g01
General protein information	Preferred Names STE20-like kinase a Names STE20-like serine/threonine-protein kinase fk52g01
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001145601.1	NP_001139073.1	STE20-like serine/threonine-protein kinase
XM_005156872.3	XP_005156929.1	STE20-like serine/threonine-protein kinase isoform X2
XM_005156871.3	XP_005156928.1	STE20-like serine/threonine-protein kinase isoform X1
XM_005156871.4	XP_005156928.1	STE20-like serine/threonine-protein kinase isoform X1
XM_005156872.4	XP_005156929.1	STE20-like serine/threonine-protein kinase isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slk	NP_033315.2
Canis lupus familiaris (dog)	SLK	XP_544006.2
Pan troglodytes (chimpanzee)	SLK	XP_508017.2
Rattus norvegicus (Norway rat)	Slk	NP_062222.2
Gallus gallus (chicken)	SLK	XP_004942322.1
	SLK	XP_001114162.1
Danio rerio (zebrafish)	slka	NP_001139073.1
Homo sapiens (human)	SLK	NP_055535.2
Bos taurus (cattle)	SLK	NP_001193006.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following slka gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slka cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36643	NM_001145601.1 Latest version!	Danio rerio STE20-like kinase a (slka), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa18809	XM_005156871.4 Latest version!	Danio rerio STE20-like kinase a (slka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa18810	XM_005156872.4 Latest version!	Danio rerio STE20-like kinase a (slka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
ODa18809	XM_005156871.3	Danio rerio STE20-like kinase a (slka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa18810	XM_005156872.3	Danio rerio STE20-like kinase a (slka), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slka ( NM_001145601.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139073.1 Danio rerio STE20-like kinase a (slka), mRNA.

Price & Availability↑

CloneID	ODa36643
Clone ID Related Accession (Same CDS sequence)	NM_001145601.1
Accession Version	NM_001145601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	STE20-like serine/threonine-protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929005.7. On Mar 5, 2009 this sequence version replaced XM_692194.2. ##Evidence-Data-START## Transcript exon combination :: GFIL01038951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGAGCA AGAGTTTCTC CAGAGACAGC AGCAGGAGCT GGATGGAGCT 
CTGAAGAAGA TCATCCAGCA GCACAAACTG GAGATCGCCA CTATAGAAAG AGACTGTCTC 
AACCACAAGC AGCAGCTCAT GAGAGCAAGA GAAGCAGCCA TGTGGGAGCT GGAGGAGCGC 
CACCTGCAGG AGAAACACCA GTTACTGAAG CAGCAGCTGA AGGATCAATA CTTCATGCAG 
AGACATCAGC TCCTCAAGAG ACATGACAAG GAGATGGAGC AGATGCAGGG TTATAACCAG 
CGGTTGATTG AGGAGATGAA GAACAGACAG GCGCAGGAAC GAGCTCGACT GCCGAAGATC 
CAGCGCAGTG AAGCCAAGAC GCGGATGGCC ATGTTTAAGA AGAGCCTCCG CATCACTGGC 
ACCGGGACGC CAGAGCAGGA TAGAGAAAAA ATCAAACAGT TTGCAGTTCA GGAGGACAAG 
AGACAGAAGA ATGAGAGACT TCATCAGCAT CAAAAACATG AGAACCAGAT GAGAGACCTG 
CAGCTGCAGT GCGACTCCAA CATCAGAGAA CTTCAGCAGA TGCAGAATGA GAAATGTCAC 
CTGCTGATTG AGCATGAGAC TCAGAAGCTG AAGGAGCTTG ATGAGGAACA CAGTCAGGAG 
CTAAAAGAAT GGAGGGAGAA GCTTCGGCCA CGGAAGAAGG CTCTGGAGGA AGAGTTCACC 
CGTAAACTCC AGGAGCAGGA AGTCTTCTTT AAGATGAGCG GAGAGTCCGA ATGTCTCAAT 
CCCAGCACCC AGAGCAGAGT CTCGAAATTC TACCCTATTC CTAGTGTGCA CTCCTCCGGC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139073.1
CDS	1..3606
Translation	MSFFNFRKIFKLGTEKKKKQYEHVNRDVNPEDFWEIVGELGDGAFGKVYKAQNKQTGILAAAKVIDTKTEEELEDYMVEIDILASCDHQYIVKLLDAFYYESKLWILIEFCAGGAVDAVMLELERPLTEPQIRVVCKQSLEALVYLHENKIIHRDLKAGNILFTSDGNIKLADFGVSAKNTKTLQRRDSFIGTPYWMAPEVVMCETSKDRPYDYKADIWSLGVTLIELAQIEPPNHEMNPMRVLLKIAKSEPPTLASPSRWSPEFSDFLRKALDKNVDNRWSALQLLQHPFVSSLVDNKPLRELIAEAKAEVFEEFEEGKEEEEEEEPEPQSAVQGHKRALSDTSVGSSEDERQNQNTPTLESVTERAEPERKPEDRACDKTSAMVVDANESNHLEDGKIVEANVSDTSAEELRSGDELISKQEESPMPVSTEGGVTDQPNAPTANDTNLQEIVTVSEETGEEEKKEVKVEEKLEKKEEESEEINMKAKAQSITDEEKEHALPSDEAEPKNKSDGVTEEAITELIEVTEKTEDKLENEEEVHGKEPEEDKTVTSADVPTEKLKEQTETLPDVSTEEVKKEQPEASPDVSTEEVKEQPETLPGASSEEVKKEQPETLPDASSEEVKKQPEPSPKGSTEEVKEEQPETSPEVSKEVKEDQLVPEDQNKTEEKAEITEPRGVDVVEDISLSKVPVVVINGVKEEEVSATEEPAEAPEDRPEIQEAPAMPESDHNTVDVAKPDAEKEKDSDSGSSSAADTNSIDINLSISSFLSKSKEGSVSIQDIKRQKKTLKKTRKFLVDGVEVSVTTSKIVTDNDTKSEELRFLRRQELRELRLLQKEEQRAQQQLSNKLQQQKEQLFRRFEQEMSGKKRQYDQEVENLEKQQKQTIERLEQDHTNRLRDEAKRIKAEQDKELSKFQNMLKNRKKEEQEFLQRQQQELDGALKKIIQQHKLEIATIERDCLNHKQQLMRAREAAMWELEERHLQEKHQLLKQQLKDQYFMQRHQLLKRHDKEMEQMQGYNQRLIEEMKNRQAQERARLPKIQRSEAKTRMAMFKKSLRITGTGTPEQDREKIKQFAVQEDKRQKNERLHQHQKHENQMRDLQLQCDSNIRELQQMQNEKCHLLIEHETQKLKELDEEHSQELKEWREKLRPRKKALEEEFTRKLQEQEVFFKMSGESECLNPSTQSRVSKFYPIPSVHSSGS

Target ORF information:

RefSeq Version	NM_001145601.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio STE20-like kinase a (slka), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145601.1

ORF Insert Sequence:

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGAGCA AGAGTTTCTC CAGAGACAGC AGCAGGAGCT GGATGGAGCT 
CTGAAGAAGA TCATCCAGCA GCACAAACTG GAGATCGCCA CTATAGAAAG AGACTGTCTC 
AACCACAAGC AGCAGCTCAT GAGAGCAAGA GAAGCAGCCA TGTGGGAGCT GGAGGAGCGC 
CACCTGCAGG AGAAACACCA GTTACTGAAG CAGCAGCTGA AGGATCAATA CTTCATGCAG 
AGACATCAGC TCCTCAAGAG ACATGACAAG GAGATGGAGC AGATGCAGGG TTATAACCAG 
CGGTTGATTG AGGAGATGAA GAACAGACAG GCGCAGGAAC GAGCTCGACT GCCGAAGATC 
CAGCGCAGTG AAGCCAAGAC GCGGATGGCC ATGTTTAAGA AGAGCCTCCG CATCACTGGC 
ACCGGGACGC CAGAGCAGGA TAGAGAAAAA ATCAAACAGT TTGCAGTTCA GGAGGACAAG 
AGACAGAAGA ATGAGAGACT TCATCAGCAT CAAAAACATG AGAACCAGAT GAGAGACCTG 
CAGCTGCAGT GCGACTCCAA CATCAGAGAA CTTCAGCAGA TGCAGAATGA GAAATGTCAC 
CTGCTGATTG AGCATGAGAC TCAGAAGCTG AAGGAGCTTG ATGAGGAACA CAGTCAGGAG 
CTAAAAGAAT GGAGGGAGAA GCTTCGGCCA CGGAAGAAGG CTCTGGAGGA AGAGTTCACC 
CGTAAACTCC AGGAGCAGGA AGTCTTCTTT AAGATGAGCG GAGAGTCCGA ATGTCTCAAT 
CCCAGCACCC AGAGCAGAGT CTCGAAATTC TACCCTATTC CTAGTGTGCA CTCCTCCGGC 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slka ( XM_005156871.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156928.1 Danio rerio STE20-like kinase a (slka), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18809
Clone ID Related Accession (Same CDS sequence)	XM_005156871.3 , XM_005156871.4
Accession Version	XM_005156871.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	STE20-like serine/threonine-protein kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGTCAA ACAAGAGGTC GGACTGTCCC CCAAACACAT GAGAAAAGAG 
CTCATGAAAC GCTTAAAAGA AGACCTAGCT GCCATTCAGC ATGGAGAGGA GCAAGAGTTT 
CTCCAGAGAC AGCAGCAGGA GCTGGATGGA GCTCTGAAGA AGATCATCCA GCAGCACAAA 
CTGGAGATCG CCACTATAGA AAGAGACTGT CTCAACCACA AGCAGCAGCT CATGAGAGCA 
AGAGAAGCAG CCATGTGGGA GCTGGAGGAG CGCCACCTGC AGGAGAAACA CCAGTTACTG 
AAGCAGCAGC TGAAGGATCA ATACTTCATG CAGAGACATC AGCTCCTCAA GAGACATGAC 
AAGGAGATGG AGCAGATGCA GGGTTATAAC CAGCGGTTGA TTGAGGAGAT GAAGAACAGA 
CAGGCGCAGG AACGAGCTCG ACTGCCGAAG ATCCAGCGCA GTGAAGCCAA GACGCGGATG 
GCCATGTTTA AGAAGAGCCT CCGCATCACT GGCACCGGGA CGCCAGAGCA GGATAGAGAA 
AAAATCAAAC AGTTTGCAGT TCAGGAGGAC AAGAGACAGA AGAATGAGAG ACTTCATCAG 
CATCAAAAAC ATGAGAACCA GATGAGAGAC CTGCAGCTGC AGTGCGACTC CAACATCAGA 
GAACTTCAGC AGATGCAGAA TGAGAAATGT CACCTGCTGA TTGAGCATGA GACTCAGAAG 
CTGAAGGAGC TTGATGAGGA ACACAGTCAG GAGCTAAAAG AATGGAGGGA GAAGCTTCGG 
CCACGGAAGA AGGCTCTGGA GGAAGAGTTC ACCCGTAAAC TCCAGGAGCA GGAAGTCTTC 
TTTAAGATGA GCGGAGAGTC CGAATGTCTC AATCCCAGCA CCCAGAGCAG AGTCTCGAAA 
TTCTACCCTA TTCCTAGTGT GCACTCCTCC GGCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156928.1
CDS	778..4476
Translation	MSFFNFRKIFKLGTEKKKKQYEHVNRDVNPEDFWEIVGELGDGAFGKVYKAQNKQTGILAAAKVIDTKTEEELEDYMVEIDILASCDHQYIVKLLDAFYYESKLWILIEFCAGGAVDAVMLELERPLTEPQIRVVCKQSLEALVYLHENKIIHRDLKAGNILFTSDGNIKLADFGVSAKNTKTLQRRDSFIGTPYWMAPEVVMCETSKDRPYDYKADIWSLGVTLIELAQIEPPNHEMNPMRVLLKIAKSEPPTLASPSRWSPEFSDFLRKALDKNVDNRWSALQLLQHPFVSSLVDNKPLRELIAEAKAEVFEEFEEGKEEEEEEEPEPQSAVQGHKRALSDTSVGSSEDERQNQNTPTLESVTERAEPERKPEDRACDKTSAMVVDANESNHLEDGKIVEANVSDTSAEELRSGDELISKQEESPMPVSTEGGVTDQPNAPTANDTNLQEIVTVSEETGEEEKKEVKVEEKLEKKEEESEEINMKAKAQSITDEEKEHALPSDEAEPKNKSDGVTEEAITELIEVTEKTEDKLENEEEVHGKEPEEDKTVTSADVPTEKLKEQTETLPDVSTEEVKKEQPEASPDVSTEEVKEQPETLPGASSEEVKKEQPETLPDASSEEVKKQPEPSPKGSTEEVKEEQPETSPEVSKEVKEDQLVPEDQNKTEEKAEITEPRGVDVVEDISLSKVPVVVINGVKEEEVSATEEPAEAPEDRPEIQEAPAMPESDHNTVDVAKPDAEKEKDSDSGSSSAADTNSIDINLSISSFLSKSKEGSVSIQDIKRQKKTLKKTRKFLVDGVEVSVTTSKIVTDNDTKSEELRFLRRQELRELRLLQKEEQRAQQQLSNKLQQQKEQLFRRFEQEMSGKKRQYDQEVENLEKQQKQTIERLEQDHTNRLRDEAKRIKAEQDKELSKFQNMLKNRKKEVKQEVGLSPKHMRKELMKRLKEDLAAIQHGEEQEFLQRQQQELDGALKKIIQQHKLEIATIERDCLNHKQQLMRAREAAMWELEERHLQEKHQLLKQQLKDQYFMQRHQLLKRHDKEMEQMQGYNQRLIEEMKNRQAQERARLPKIQRSEAKTRMAMFKKSLRITGTGTPEQDREKIKQFAVQEDKRQKNERLHQHQKHENQMRDLQLQCDSNIRELQQMQNEKCHLLIEHETQKLKELDEEHSQELKEWREKLRPRKKALEEEFTRKLQEQEVFFKMSGESECLNPSTQSRVSKFYPIPSVHSSGS

Target ORF information:

RefSeq Version	XM_005156871.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio STE20-like kinase a (slka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156871.3

ORF Insert Sequence:

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGTCAA ACAAGAGGTC GGACTGTCCC CCAAACACAT GAGAAAAGAG 
CTCATGAAAC GCTTAAAAGA AGACCTAGCT GCCATTCAGC ATGGAGAGGA GCAAGAGTTT 
CTCCAGAGAC AGCAGCAGGA GCTGGATGGA GCTCTGAAGA AGATCATCCA GCAGCACAAA 
CTGGAGATCG CCACTATAGA AAGAGACTGT CTCAACCACA AGCAGCAGCT CATGAGAGCA 
AGAGAAGCAG CCATGTGGGA GCTGGAGGAG CGCCACCTGC AGGAGAAACA CCAGTTACTG 
AAGCAGCAGC TGAAGGATCA ATACTTCATG CAGAGACATC AGCTCCTCAA GAGACATGAC 
AAGGAGATGG AGCAGATGCA GGGTTATAAC CAGCGGTTGA TTGAGGAGAT GAAGAACAGA 
CAGGCGCAGG AACGAGCTCG ACTGCCGAAG ATCCAGCGCA GTGAAGCCAA GACGCGGATG 
GCCATGTTTA AGAAGAGCCT CCGCATCACT GGCACCGGGA CGCCAGAGCA GGATAGAGAA 
AAAATCAAAC AGTTTGCAGT TCAGGAGGAC AAGAGACAGA AGAATGAGAG ACTTCATCAG 
CATCAAAAAC ATGAGAACCA GATGAGAGAC CTGCAGCTGC AGTGCGACTC CAACATCAGA 
GAACTTCAGC AGATGCAGAA TGAGAAATGT CACCTGCTGA TTGAGCATGA GACTCAGAAG 
CTGAAGGAGC TTGATGAGGA ACACAGTCAG GAGCTAAAAG AATGGAGGGA GAAGCTTCGG 
CCACGGAAGA AGGCTCTGGA GGAAGAGTTC ACCCGTAAAC TCCAGGAGCA GGAAGTCTTC 
TTTAAGATGA GCGGAGAGTC CGAATGTCTC AATCCCAGCA CCCAGAGCAG AGTCTCGAAA 
TTCTACCCTA TTCCTAGTGT GCACTCCTCC GGCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slka ( XM_005156871.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156928.1 Danio rerio STE20-like kinase a (slka), transcript variant X1, mRNA.

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CloneID	ODa18809
Clone ID Related Accession (Same CDS sequence)	XM_005156871.3 , XM_005156871.4
Accession Version	XM_005156871.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	STE20-like serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156871.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGTCAA ACAAGAGGTC GGACTGTCCC CCAAACACAT GAGAAAAGAG 
CTCATGAAAC GCTTAAAAGA AGACCTAGCT GCCATTCAGC ATGGAGAGGA GCAAGAGTTT 
CTCCAGAGAC AGCAGCAGGA GCTGGATGGA GCTCTGAAGA AGATCATCCA GCAGCACAAA 
CTGGAGATCG CCACTATAGA AAGAGACTGT CTCAACCACA AGCAGCAGCT CATGAGAGCA 
AGAGAAGCAG CCATGTGGGA GCTGGAGGAG CGCCACCTGC AGGAGAAACA CCAGTTACTG 
AAGCAGCAGC TGAAGGATCA ATACTTCATG CAGAGACATC AGCTCCTCAA GAGACATGAC 
AAGGAGATGG AGCAGATGCA GGGTTATAAC CAGCGGTTGA TTGAGGAGAT GAAGAACAGA 
CAGGCGCAGG AACGAGCTCG ACTGCCGAAG ATCCAGCGCA GTGAAGCCAA GACGCGGATG 
GCCATGTTTA AGAAGAGCCT CCGCATCACT GGCACCGGGA CGCCAGAGCA GGATAGAGAA 
AAAATCAAAC AGTTTGCAGT TCAGGAGGAC AAGAGACAGA AGAATGAGAG ACTTCATCAG 
CATCAAAAAC ATGAGAACCA GATGAGAGAC CTGCAGCTGC AGTGCGACTC CAACATCAGA 
GAACTTCAGC AGATGCAGAA TGAGAAATGT CACCTGCTGA TTGAGCATGA GACTCAGAAG 
CTGAAGGAGC TTGATGAGGA ACACAGTCAG GAGCTAAAAG AATGGAGGGA GAAGCTTCGG 
CCACGGAAGA AGGCTCTGGA GGAAGAGTTC ACCCGTAAAC TCCAGGAGCA GGAAGTCTTC 
TTTAAGATGA GCGGAGAGTC CGAATGTCTC AATCCCAGCA CCCAGAGCAG AGTCTCGAAA 
TTCTACCCTA TTCCTAGTGT GCACTCCTCC GGCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156928.1
CDS	785..4483
Translation	MSFFNFRKIFKLGTEKKKKQYEHVNRDVNPEDFWEIVGELGDGAFGKVYKAQNKQTGILAAAKVIDTKTEEELEDYMVEIDILASCDHQYIVKLLDAFYYESKLWILIEFCAGGAVDAVMLELERPLTEPQIRVVCKQSLEALVYLHENKIIHRDLKAGNILFTSDGNIKLADFGVSAKNTKTLQRRDSFIGTPYWMAPEVVMCETSKDRPYDYKADIWSLGVTLIELAQIEPPNHEMNPMRVLLKIAKSEPPTLASPSRWSPEFSDFLRKALDKNVDNRWSALQLLQHPFVSSLVDNKPLRELIAEAKAEVFEEFEEGKEEEEEEEPEPQSAVQGHKRALSDTSVGSSEDERQNQNTPTLESVTERAEPERKPEDRACDKTSAMVVDANESNHLEDGKIVEANVSDTSAEELRSGDELISKQEESPMPVSTEGGVTDQPNAPTANDTNLQEIVTVSEETGEEEKKEVKVEEKLEKKEEESEEINMKAKAQSITDEEKEHALPSDEAEPKNKSDGVTEEAITELIEVTEKTEDKLENEEEVHGKEPEEDKTVTSADVPTEKLKEQTETLPDVSTEEVKKEQPEASPDVSTEEVKEQPETLPGASSEEVKKEQPETLPDASSEEVKKQPEPSPKGSTEEVKEEQPETSPEVSKEVKEDQLVPEDQNKTEEKAEITEPRGVDVVEDISLSKVPVVVINGVKEEEVSATEEPAEAPEDRPEIQEAPAMPESDHNTVDVAKPDAEKEKDSDSGSSSAADTNSIDINLSISSFLSKSKEGSVSIQDIKRQKKTLKKTRKFLVDGVEVSVTTSKIVTDNDTKSEELRFLRRQELRELRLLQKEEQRAQQQLSNKLQQQKEQLFRRFEQEMSGKKRQYDQEVENLEKQQKQTIERLEQDHTNRLRDEAKRIKAEQDKELSKFQNMLKNRKKEVKQEVGLSPKHMRKELMKRLKEDLAAIQHGEEQEFLQRQQQELDGALKKIIQQHKLEIATIERDCLNHKQQLMRAREAAMWELEERHLQEKHQLLKQQLKDQYFMQRHQLLKRHDKEMEQMQGYNQRLIEEMKNRQAQERARLPKIQRSEAKTRMAMFKKSLRITGTGTPEQDREKIKQFAVQEDKRQKNERLHQHQKHENQMRDLQLQCDSNIRELQQMQNEKCHLLIEHETQKLKELDEEHSQELKEWREKLRPRKKALEEEFTRKLQEQEVFFKMSGESECLNPSTQSRVSKFYPIPSVHSSGS

Target ORF information:

RefSeq Version	XM_005156871.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio STE20-like kinase a (slka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156871.4

ORF Insert Sequence:

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGTCAA ACAAGAGGTC GGACTGTCCC CCAAACACAT GAGAAAAGAG 
CTCATGAAAC GCTTAAAAGA AGACCTAGCT GCCATTCAGC ATGGAGAGGA GCAAGAGTTT 
CTCCAGAGAC AGCAGCAGGA GCTGGATGGA GCTCTGAAGA AGATCATCCA GCAGCACAAA 
CTGGAGATCG CCACTATAGA AAGAGACTGT CTCAACCACA AGCAGCAGCT CATGAGAGCA 
AGAGAAGCAG CCATGTGGGA GCTGGAGGAG CGCCACCTGC AGGAGAAACA CCAGTTACTG 
AAGCAGCAGC TGAAGGATCA ATACTTCATG CAGAGACATC AGCTCCTCAA GAGACATGAC 
AAGGAGATGG AGCAGATGCA GGGTTATAAC CAGCGGTTGA TTGAGGAGAT GAAGAACAGA 
CAGGCGCAGG AACGAGCTCG ACTGCCGAAG ATCCAGCGCA GTGAAGCCAA GACGCGGATG 
GCCATGTTTA AGAAGAGCCT CCGCATCACT GGCACCGGGA CGCCAGAGCA GGATAGAGAA 
AAAATCAAAC AGTTTGCAGT TCAGGAGGAC AAGAGACAGA AGAATGAGAG ACTTCATCAG 
CATCAAAAAC ATGAGAACCA GATGAGAGAC CTGCAGCTGC AGTGCGACTC CAACATCAGA 
GAACTTCAGC AGATGCAGAA TGAGAAATGT CACCTGCTGA TTGAGCATGA GACTCAGAAG 
CTGAAGGAGC TTGATGAGGA ACACAGTCAG GAGCTAAAAG AATGGAGGGA GAAGCTTCGG 
CCACGGAAGA AGGCTCTGGA GGAAGAGTTC ACCCGTAAAC TCCAGGAGCA GGAAGTCTTC 
TTTAAGATGA GCGGAGAGTC CGAATGTCTC AATCCCAGCA CCCAGAGCAG AGTCTCGAAA 
TTCTACCCTA TTCCTAGTGT GCACTCCTCC GGCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slka ( XM_005156872.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156929.1 Danio rerio STE20-like kinase a (slka), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18810
Clone ID Related Accession (Same CDS sequence)	XM_005156872.3 , XM_005156872.4
Accession Version	XM_005156872.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	STE20-like serine/threonine-protein kinase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156872.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGGTGT GGCCCAGGTT ATGATACAGT CTTTTCAGTT GTCCTCATGT 
GCTCTGTTCA ACGCTCAGAT GCAGGATGAG CAAGAGTTTC TCCAGAGACA GCAGCAGGAG 
CTGGATGGAG CTCTGAAGAA GATCATCCAG CAGCACAAAC TGGAGATCGC CACTATAGAA 
AGAGACTGTC TCAACCACAA GCAGCAGCTC ATGAGAGCAA GAGAAGCAGC CATGTGGGAG 
CTGGAGGAGC GCCACCTGCA GGAGAAACAC CAGTTACTGA AGCAGCAGCT GAAGGATCAA 
TACTTCATGC AGAGACATCA GCTCCTCAAG AGACATGACA AGGAGATGGA GCAGATGCAG 
GGTTATAACC AGCGGTTGAT TGAGGAGATG AAGAACAGAC AGGCGCAGGA ACGAGCTCGA 
CTGCCGAAGA TCCAGCGCAG TGAAGCCAAG ACGCGGATGG CCATGTTTAA GAAGAGCCTC 
CGCATCACTG GCACCGGGAC GCCAGAGCAG GATAGAGAAA AAATCAAACA GTTTGCAGTT 
CAGGAGGACA AGAGACAGAA GAATGAGAGA CTTCATCAGC ATCAAAAACA TGAGAACCAG 
ATGAGAGACC TGCAGCTGCA GTGCGACTCC AACATCAGAG AACTTCAGCA GATGCAGAAT 
GAGAAATGTC ACCTGCTGAT TGAGCATGAG ACTCAGAAGC TGAAGGAGCT TGATGAGGAA 
CACAGTCAGG AGCTAAAAGA ATGGAGGGAG AAGCTTCGGC CACGGAAGAA GGCTCTGGAG 
GAAGAGTTCA CCCGTAAACT CCAGGAGCAG GAAGTCTTCT TTAAGATGAG CGGAGAGTCC 
GAATGTCTCA ATCCCAGCAC CCAGAGCAGA GTCTCGAAAT TCTACCCTAT TCCTAGTGTG 
CACTCCTCCG GCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156929.1
CDS	778..4455
Translation	MSFFNFRKIFKLGTEKKKKQYEHVNRDVNPEDFWEIVGELGDGAFGKVYKAQNKQTGILAAAKVIDTKTEEELEDYMVEIDILASCDHQYIVKLLDAFYYESKLWILIEFCAGGAVDAVMLELERPLTEPQIRVVCKQSLEALVYLHENKIIHRDLKAGNILFTSDGNIKLADFGVSAKNTKTLQRRDSFIGTPYWMAPEVVMCETSKDRPYDYKADIWSLGVTLIELAQIEPPNHEMNPMRVLLKIAKSEPPTLASPSRWSPEFSDFLRKALDKNVDNRWSALQLLQHPFVSSLVDNKPLRELIAEAKAEVFEEFEEGKEEEEEEEPEPQSAVQGHKRALSDTSVGSSEDERQNQNTPTLESVTERAEPERKPEDRACDKTSAMVVDANESNHLEDGKIVEANVSDTSAEELRSGDELISKQEESPMPVSTEGGVTDQPNAPTANDTNLQEIVTVSEETGEEEKKEVKVEEKLEKKEEESEEINMKAKAQSITDEEKEHALPSDEAEPKNKSDGVTEEAITELIEVTEKTEDKLENEEEVHGKEPEEDKTVTSADVPTEKLKEQTETLPDVSTEEVKKEQPEASPDVSTEEVKEQPETLPGASSEEVKKEQPETLPDASSEEVKKQPEPSPKGSTEEVKEEQPETSPEVSKEVKEDQLVPEDQNKTEEKAEITEPRGVDVVEDISLSKVPVVVINGVKEEEVSATEEPAEAPEDRPEIQEAPAMPESDHNTVDVAKPDAEKEKDSDSGSSSAADTNSIDINLSISSFLSKSKEGSVSIQDIKRQKKTLKKTRKFLVDGVEVSVTTSKIVTDNDTKSEELRFLRRQELRELRLLQKEEQRAQQQLSNKLQQQKEQLFRRFEQEMSGKKRQYDQEVENLEKQQKQTIERLEQDHTNRLRDEAKRIKAEQDKELSKFQNMLKNRKKEGVAQVMIQSFQLSSCALFNAQMQDEQEFLQRQQQELDGALKKIIQQHKLEIATIERDCLNHKQQLMRAREAAMWELEERHLQEKHQLLKQQLKDQYFMQRHQLLKRHDKEMEQMQGYNQRLIEEMKNRQAQERARLPKIQRSEAKTRMAMFKKSLRITGTGTPEQDREKIKQFAVQEDKRQKNERLHQHQKHENQMRDLQLQCDSNIRELQQMQNEKCHLLIEHETQKLKELDEEHSQELKEWREKLRPRKKALEEEFTRKLQEQEVFFKMSGESECLNPSTQSRVSKFYPIPSVHSSGS

Target ORF information:

RefSeq Version	XM_005156872.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio STE20-like kinase a (slka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156872.3

ORF Insert Sequence:

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGGTGT GGCCCAGGTT ATGATACAGT CTTTTCAGTT GTCCTCATGT 
GCTCTGTTCA ACGCTCAGAT GCAGGATGAG CAAGAGTTTC TCCAGAGACA GCAGCAGGAG 
CTGGATGGAG CTCTGAAGAA GATCATCCAG CAGCACAAAC TGGAGATCGC CACTATAGAA 
AGAGACTGTC TCAACCACAA GCAGCAGCTC ATGAGAGCAA GAGAAGCAGC CATGTGGGAG 
CTGGAGGAGC GCCACCTGCA GGAGAAACAC CAGTTACTGA AGCAGCAGCT GAAGGATCAA 
TACTTCATGC AGAGACATCA GCTCCTCAAG AGACATGACA AGGAGATGGA GCAGATGCAG 
GGTTATAACC AGCGGTTGAT TGAGGAGATG AAGAACAGAC AGGCGCAGGA ACGAGCTCGA 
CTGCCGAAGA TCCAGCGCAG TGAAGCCAAG ACGCGGATGG CCATGTTTAA GAAGAGCCTC 
CGCATCACTG GCACCGGGAC GCCAGAGCAG GATAGAGAAA AAATCAAACA GTTTGCAGTT 
CAGGAGGACA AGAGACAGAA GAATGAGAGA CTTCATCAGC ATCAAAAACA TGAGAACCAG 
ATGAGAGACC TGCAGCTGCA GTGCGACTCC AACATCAGAG AACTTCAGCA GATGCAGAAT 
GAGAAATGTC ACCTGCTGAT TGAGCATGAG ACTCAGAAGC TGAAGGAGCT TGATGAGGAA 
CACAGTCAGG AGCTAAAAGA ATGGAGGGAG AAGCTTCGGC CACGGAAGAA GGCTCTGGAG 
GAAGAGTTCA CCCGTAAACT CCAGGAGCAG GAAGTCTTCT TTAAGATGAG CGGAGAGTCC 
GAATGTCTCA ATCCCAGCAC CCAGAGCAGA GTCTCGAAAT TCTACCCTAT TCCTAGTGTG 
CACTCCTCCG GCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slka ( XM_005156872.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156929.1 Danio rerio STE20-like kinase a (slka), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18810
Clone ID Related Accession (Same CDS sequence)	XM_005156872.3 , XM_005156872.4
Accession Version	XM_005156872.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	STE20-like serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156872.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGGTGT GGCCCAGGTT ATGATACAGT CTTTTCAGTT GTCCTCATGT 
GCTCTGTTCA ACGCTCAGAT GCAGGATGAG CAAGAGTTTC TCCAGAGACA GCAGCAGGAG 
CTGGATGGAG CTCTGAAGAA GATCATCCAG CAGCACAAAC TGGAGATCGC CACTATAGAA 
AGAGACTGTC TCAACCACAA GCAGCAGCTC ATGAGAGCAA GAGAAGCAGC CATGTGGGAG 
CTGGAGGAGC GCCACCTGCA GGAGAAACAC CAGTTACTGA AGCAGCAGCT GAAGGATCAA 
TACTTCATGC AGAGACATCA GCTCCTCAAG AGACATGACA AGGAGATGGA GCAGATGCAG 
GGTTATAACC AGCGGTTGAT TGAGGAGATG AAGAACAGAC AGGCGCAGGA ACGAGCTCGA 
CTGCCGAAGA TCCAGCGCAG TGAAGCCAAG ACGCGGATGG CCATGTTTAA GAAGAGCCTC 
CGCATCACTG GCACCGGGAC GCCAGAGCAG GATAGAGAAA AAATCAAACA GTTTGCAGTT 
CAGGAGGACA AGAGACAGAA GAATGAGAGA CTTCATCAGC ATCAAAAACA TGAGAACCAG 
ATGAGAGACC TGCAGCTGCA GTGCGACTCC AACATCAGAG AACTTCAGCA GATGCAGAAT 
GAGAAATGTC ACCTGCTGAT TGAGCATGAG ACTCAGAAGC TGAAGGAGCT TGATGAGGAA 
CACAGTCAGG AGCTAAAAGA ATGGAGGGAG AAGCTTCGGC CACGGAAGAA GGCTCTGGAG 
GAAGAGTTCA CCCGTAAACT CCAGGAGCAG GAAGTCTTCT TTAAGATGAG CGGAGAGTCC 
GAATGTCTCA ATCCCAGCAC CCAGAGCAGA GTCTCGAAAT TCTACCCTAT TCCTAGTGTG 
CACTCCTCCG GCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156929.1
CDS	785..4462
Translation	MSFFNFRKIFKLGTEKKKKQYEHVNRDVNPEDFWEIVGELGDGAFGKVYKAQNKQTGILAAAKVIDTKTEEELEDYMVEIDILASCDHQYIVKLLDAFYYESKLWILIEFCAGGAVDAVMLELERPLTEPQIRVVCKQSLEALVYLHENKIIHRDLKAGNILFTSDGNIKLADFGVSAKNTKTLQRRDSFIGTPYWMAPEVVMCETSKDRPYDYKADIWSLGVTLIELAQIEPPNHEMNPMRVLLKIAKSEPPTLASPSRWSPEFSDFLRKALDKNVDNRWSALQLLQHPFVSSLVDNKPLRELIAEAKAEVFEEFEEGKEEEEEEEPEPQSAVQGHKRALSDTSVGSSEDERQNQNTPTLESVTERAEPERKPEDRACDKTSAMVVDANESNHLEDGKIVEANVSDTSAEELRSGDELISKQEESPMPVSTEGGVTDQPNAPTANDTNLQEIVTVSEETGEEEKKEVKVEEKLEKKEEESEEINMKAKAQSITDEEKEHALPSDEAEPKNKSDGVTEEAITELIEVTEKTEDKLENEEEVHGKEPEEDKTVTSADVPTEKLKEQTETLPDVSTEEVKKEQPEASPDVSTEEVKEQPETLPGASSEEVKKEQPETLPDASSEEVKKQPEPSPKGSTEEVKEEQPETSPEVSKEVKEDQLVPEDQNKTEEKAEITEPRGVDVVEDISLSKVPVVVINGVKEEEVSATEEPAEAPEDRPEIQEAPAMPESDHNTVDVAKPDAEKEKDSDSGSSSAADTNSIDINLSISSFLSKSKEGSVSIQDIKRQKKTLKKTRKFLVDGVEVSVTTSKIVTDNDTKSEELRFLRRQELRELRLLQKEEQRAQQQLSNKLQQQKEQLFRRFEQEMSGKKRQYDQEVENLEKQQKQTIERLEQDHTNRLRDEAKRIKAEQDKELSKFQNMLKNRKKEGVAQVMIQSFQLSSCALFNAQMQDEQEFLQRQQQELDGALKKIIQQHKLEIATIERDCLNHKQQLMRAREAAMWELEERHLQEKHQLLKQQLKDQYFMQRHQLLKRHDKEMEQMQGYNQRLIEEMKNRQAQERARLPKIQRSEAKTRMAMFKKSLRITGTGTPEQDREKIKQFAVQEDKRQKNERLHQHQKHENQMRDLQLQCDSNIRELQQMQNEKCHLLIEHETQKLKELDEEHSQELKEWREKLRPRKKALEEEFTRKLQEQEVFFKMSGESECLNPSTQSRVSKFYPIPSVHSSGS

Target ORF information:

RefSeq Version	XM_005156872.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio STE20-like kinase a (slka), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156872.4

ORF Insert Sequence:

ATGTCCTTTT TCAATTTTCG GAAGATATTT AAGTTGGGAA CGGAGAAGAA GAAGAAACAG 
TATGAGCATG TCAACAGAGA CGTCAACCCC GAGGATTTTT GGGAGATCGT GGGAGAGCTT 
GGGGATGGAG CCTTTGGCAA AGTCTACAAG GCTCAGAACA AACAGACTGG GATCTTGGCT 
GCAGCTAAAG TGATCGATAC CAAAACAGAA GAGGAGCTTG AGGACTACAT GGTGGAGATC 
GACATCCTCG CCTCCTGTGA CCATCAGTAT ATAGTCAAAC TGCTGGATGC TTTCTATTAT 
GAGAGCAAAC TCTGGATTCT CATTGAGTTT TGTGCTGGAG GAGCGGTAGA CGCTGTGATG 
TTGGAGCTGG AGCGGCCACT GACAGAGCCT CAGATCCGGG TGGTGTGTAA ACAGTCGCTG 
GAGGCGCTGG TTTACCTCCA TGAGAATAAG ATTATACACC GGGACCTGAA GGCTGGAAAT 
ATTCTCTTTA CCTCAGATGG AAACATCAAA TTGGCTGACT TTGGAGTGTC TGCAAAGAAT 
ACAAAGACCC TACAGAGAAG AGACTCTTTC ATTGGAACGC CATACTGGAT GGCACCCGAG 
GTGGTGATGT GTGAGACGTC AAAGGACAGA CCATACGACT ACAAAGCGGA CATCTGGTCC 
CTGGGGGTGA CGCTGATTGA GCTGGCACAG ATCGAGCCAC CCAACCACGA GATGAACCCA 
ATGAGAGTCC TGCTAAAAAT AGCAAAGTCT GAGCCCCCCA CTCTGGCATC TCCATCACGA 
TGGTCTCCTG AGTTCAGTGA TTTCTTGCGG AAAGCACTGG ATAAGAATGT AGACAACAGG 
TGGAGTGCCT TGCAGCTTTT ACAGCATCCA TTTGTTTCTA GTCTAGTGGA TAACAAACCA 
CTCCGAGAAC TAATTGCTGA AGCTAAAGCC GAAGTGTTTG AGGAGTTTGA AGAGGGAAAA 
GAGGAAGAGG AGGAAGAAGA GCCCGAGCCC CAATCGGCAG TGCAAGGACA CAAACGTGCA 
TTATCAGACA CCAGTGTTGG CAGCTCAGAG GATGAGAGAC AAAATCAGAA CACGCCAACT 
CTCGAGTCGG TCACAGAGAG AGCTGAACCT GAAAGAAAAC CTGAGGATCG AGCTTGTGAC 
AAGACATCTG CCATGGTGGT GGATGCTAAC GAATCTAACC ATCTTGAGGA TGGGAAGATA 
GTTGAAGCCA ATGTTTCAGA CACCAGCGCT GAGGAATTAC GTTCTGGTGA TGAGCTCATA 
TCAAAGCAAG AAGAATCTCC TATGCCAGTT AGCACAGAAG GGGGGGTTAC TGATCAGCCG 
AATGCTCCAA CAGCAAATGA CACTAACCTC CAAGAGATTG TAACTGTCAG TGAGGAAACT 
GGTGAGGAGG AAAAGAAAGA AGTGAAGGTG GAAGAGAAAC TAGAGAAGAA AGAAGAGGAG 
TCTGAAGAAA TTAATATGAA AGCCAAAGCA CAAAGCATTA CAGATGAAGA AAAGGAGCAT 
GCCTTACCTT CAGATGAGGC TGAACCGAAG AATAAATCCG ATGGTGTTAC TGAAGAAGCC 
ATTACTGAAC TAATTGAAGT TACAGAAAAA ACTGAAGACA AGCTTGAGAA TGAAGAAGAG 
GTGCATGGAA AGGAACCAGA AGAAGATAAG ACAGTTACCT CAGCTGATGT TCCTACAGAA 
AAATTGAAGG AGCAAACAGA AACTTTACCA GATGTTTCTA CAGAGGAAGT AAAGAAGGAG 
CAACCGGAAG CTTCACCAGA TGTTTCTACA GAGGAAGTAA AGGAGCAACC GGAAACTTTA 
CCAGGTGCTT CTTCAGAAGA AGTAAAGAAG GAGCAACCGG AAACTTTACC AGATGCTTCT 
TCAGAAGAAG TAAAGAAGCA ACCAGAACCT TCACCGAAAG GTTCTACAGA AGAAGTAAAA 
GAGGAGCAAC CGGAGACTTC ACCAGAAGTT TCTAAAGAAG TAAAGGAAGA TCAGTTGGTC 
CCAGAGGACC AAAATAAAAC TGAAGAAAAG GCTGAGATTA CAGAACCCAG GGGGGTAGAT 
GTGGTAGAGG ACATCTCTCT GAGTAAAGTT CCTGTTGTTG TCATTAATGG TGTGAAAGAA 
GAGGAGGTCA GTGCTACAGA AGAACCTGCA GAAGCCCCTG AAGACAGACC AGAGATCCAG 
GAAGCCCCTG CCATGCCTGA AAGTGACCAC AATACAGTAG ATGTGGCAAA ACCAGATGCC 
GAAAAGGAAA AAGACTCCGA TTCAGGCAGC AGTTCTGCTG CAGATACTAA TAGCATTGAT 
ATTAATCTGT CCATTTCCAG TTTCCTTTCC AAGAGCAAAG AAGGATCTGT TTCAATACAG 
GACATCAAAC GCCAAAAGAA AACCCTGAAA AAGACTCGCA AGTTTTTGGT CGACGGGGTG 
GAAGTCAGTG TCACCACATC CAAGATTGTC ACAGACAATG ACACCAAGAG TGAGGAACTG 
AGATTCCTGA GGCGCCAAGA GCTACGAGAA CTGAGGCTGC TGCAAAAAGA GGAGCAAAGG 
GCTCAACAGC AGCTCAGCAA TAAACTGCAG CAGCAGAAAG AGCAACTCTT CAGACGCTTT 
GAGCAGGAAA TGTCGGGAAA GAAACGGCAG TATGACCAAG AGGTGGAGAA CCTTGAGAAA 
CAGCAGAAGC AGACTATAGA GCGTCTGGAG CAGGACCACA CCAACCGACT AAGAGACGAA 
GCCAAAAGGA TCAAAGCTGA GCAGGACAAG GAGCTCTCCA AGTTCCAGAA CATGCTGAAG 
AATCGCAAAA AAGAGGGTGT GGCCCAGGTT ATGATACAGT CTTTTCAGTT GTCCTCATGT 
GCTCTGTTCA ACGCTCAGAT GCAGGATGAG CAAGAGTTTC TCCAGAGACA GCAGCAGGAG 
CTGGATGGAG CTCTGAAGAA GATCATCCAG CAGCACAAAC TGGAGATCGC CACTATAGAA 
AGAGACTGTC TCAACCACAA GCAGCAGCTC ATGAGAGCAA GAGAAGCAGC CATGTGGGAG 
CTGGAGGAGC GCCACCTGCA GGAGAAACAC CAGTTACTGA AGCAGCAGCT GAAGGATCAA 
TACTTCATGC AGAGACATCA GCTCCTCAAG AGACATGACA AGGAGATGGA GCAGATGCAG 
GGTTATAACC AGCGGTTGAT TGAGGAGATG AAGAACAGAC AGGCGCAGGA ACGAGCTCGA 
CTGCCGAAGA TCCAGCGCAG TGAAGCCAAG ACGCGGATGG CCATGTTTAA GAAGAGCCTC 
CGCATCACTG GCACCGGGAC GCCAGAGCAG GATAGAGAAA AAATCAAACA GTTTGCAGTT 
CAGGAGGACA AGAGACAGAA GAATGAGAGA CTTCATCAGC ATCAAAAACA TGAGAACCAG 
ATGAGAGACC TGCAGCTGCA GTGCGACTCC AACATCAGAG AACTTCAGCA GATGCAGAAT 
GAGAAATGTC ACCTGCTGAT TGAGCATGAG ACTCAGAAGC TGAAGGAGCT TGATGAGGAA 
CACAGTCAGG AGCTAAAAGA ATGGAGGGAG AAGCTTCGGC CACGGAAGAA GGCTCTGGAG 
GAAGAGTTCA CCCGTAAACT CCAGGAGCAG GAAGTCTTCT TTAAGATGAG CGGAGAGTCC 
GAATGTCTCA ATCCCAGCAC CCAGAGCAGA GTCTCGAAAT TCTACCCTAT TCCTAGTGTG 
CACTCCTCCG GCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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slka cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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