wrn cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wrn
Entrez Gene ID	569495
Full Name	Werner syndrome
General protein information	Preferred Names Werner syndrome Names Werner syndrome ATP-dependent helicase Werner syndrome ATP-dependent helicase homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303971.2	XP_009302246.1	Werner syndrome ATP-dependent helicase isoform X1
XM_021479248.1	XP_021334923.1	Werner syndrome ATP-dependent helicase
XM_009304279.2	XP_009302554.1	Werner syndrome ATP-dependent helicase isoform X2
XM_009303971.3	XP_009302246.2	Werner syndrome ATP-dependent helicase

Pathways from BioSystems

Homologies

Homo sapiens (human)	WRN	NP_000544.2
Mus musculus (house mouse)	Wrn	NP_035851.3
Bos taurus (cattle)	WRN	NP_001137329.2
Caenorhabditis elegans (roundworm)	wrn-1	NP_495324.2
Canis lupus familiaris (dog)	WRN	XP_539984.4
Gallus gallus (chicken)	WRN	XP_001235033.2
Xenopus tropicalis (tropical clawed frog)	wrn	XP_002939304.1
Pan troglodytes (chimpanzee)	WRN	XP_528104.2
	WRN	XP_001085031.1
Rattus norvegicus (Norway rat)	RGD1564788	XP_006253317.1
Danio rerio (zebrafish)	wrn	XP_697980.5
Oryza sativa (rice)	Os07g0681600	NP_001060655.1

Gene Ontology

The following wrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145058	XM_009303971.3 Latest version!	Danio rerio Werner syndrome (wrn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa45771	XM_009304279.2 Latest version!	Danio rerio Werner syndrome (wrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa45770	XM_021479248.1 Latest version!	Danio rerio Werner syndrome (wrn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa45770	XM_009303971.2 Latest version!	Danio rerio Werner syndrome (wrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wrn ( XM_009303971.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302246.2 Danio rerio Werner syndrome (wrn), mRNA.

Price & Availability↑

CloneID	ODa145058
Clone ID Related Accession (Same CDS sequence)	XM_009303971.3
Accession Version	XM_009303971.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Werner syndrome ATP-dependent helicase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394765.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303971.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA 
GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT 
TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG 
AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT 
CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC 
ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC 
ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT 
GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC 
AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT 
GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT 
GGTTGCTCAG GATCCTCAGC ACCCAGTTTG AAAGAGAGAG TGTTAAAAAT TGCTTGTGAT 
CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA 
CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC 
AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC 
AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA 
AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC CGAGTACGAG 
CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT 
TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG 
TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG 
CCTCAGTCTG GTGCCGAAGA CGAAGATGAA GATGTTGAGG AGAACGAGGA AGAAGGGCTA 
GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA 
CATCACAGTT TCAAACCTGT TCAGTGGAAA GTGATTCAGT CTATTCTCAC AGAGAGGAGA 
GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT 
GTTTACTGCC AGACAATCAG TGTGGTCATT TCACCACTCA TCGCCCTCAT GGAGGATCAA 
GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGATCTGC TCAGACCAAA 
AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC 
TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT 
GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG 
GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC 
GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT 
ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC 
GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG 
GGCTCTGCCA TTGTGTACTG TCCATCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA 
TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA 
GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT 
GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA 
ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT 
CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA 
AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT 
TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA 
GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT 
GAAGAGGATC TAGAGGTGCT GCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG 
GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CCATCCTCTT CCTGCGTGGT 
TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG 
GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG 
ATGGAAGTCA CAGGCCACAG CAAGTTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA 
TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA 
GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT 
ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA 
AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA 
GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA 
GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT 
AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA 
AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA 
GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA 
CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCTG TCGTGAATCA 
GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTCTTC 
CAGACAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATAGCGGTG 
GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA 
GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT 
GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG 
GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT 
GAGCCACTGG ACAAATCAAC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG 
CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC 
ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTACCTC TGAAGTCACA TGAGCCAGTT 
CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA 
CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG 
CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA 
AAGAAGAAAG GACTTTTCAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302246.2
CDS	248..4411
Translation	MGDRTLPKWMNNKPQDEHTSGTYKKNVLEDHLPYLEFGGTVVYSQERHDCSFLSEDLRSALSSGSAVGFDLEWPPSFTKGKTKKVAMVQLCASEDKCYLFHISSMSGFPPGLKMFLEDENIMKVGVGIEGDKWKLLSDYDIKLKNIVDLSDLANEKLRCCEKWSLDGLVKHLLKKQLFKDKLVRCSHWDDFSLTEDQKRYAATDAYAGLLIYQKLQEMISGCSGSSAPSLKERVLKIACDLKDLADHIPNELDCTDGAGRLVDDLSQTLTSLRCVLSNANKPTEQLVEEKPSPGLILDDTSSHHEGVPTTEEQSHSQCVENPGRPPQRECAMSLDISEYELQMLEEQARQEQLEEECILAFQTQHASGPQDLNATESDEEFDDEMMKCADDIEKINNAQMPQSGAEDEDEDVEENEEEGLDSSIPQPVPEQIKCLKMFFGHHSFKPVQWKVIQSILTERRDNLVVMATGYGKSLCFQFPPVYCQTISVVISPLIALMEDQVLQLTMSNIPACFLGSAQTKNLNEQVKRGDFRVVYMTPEFCSGNIPLLVQLDRTVGLSLIAVDEAHCISQWGHDFRSAYRELGKLKKNLPTVPFLALTATASPSIRDDIIKSLHLINPMITCTSFDRPNLYLDVNRKSGDVIQDLKRFLVKKKGGGYEFEGSAIVYCPSKKEAERVTTALFKLDIPCGVYHAGLSIKQRRETQHQFMRDEIQCVVATVAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSACHVLWMPGDMALNKFILNQSKSERFRSYKIDMMAKMEKYLNSTKCRRKLILCHFEDKRLRKVTSGILGSSQCCDNCRSGVNEEDLEVLQQDFGLCAYQLMKAISVMEERFGITAPILFLRGSCSQRVPERFRKHSLFGIGRDVSETWWKALGRELIAEKYLMEVTGHSKFTTLCKITTKGRSWLSKAEDEKHRQLLLQPNRDLFSRVCAPRRKADPAQVEPTVSNLQSPHIRPSLVTFQRTKLGSESPQIVKKGDLPSRSAARQTQIQTDVRAQTPGASAKEIQLQSELYGKLVSERQKLASIRDIPPALLATNKILLDMSKLRPCTMSSLKQVDGVSEAKAAMLTPLIDIIAKFCQTHGLQVDVSSNSASSVQTHSCRESVVGSVAQCLPDSISITYSLFQTEHKSLRQVSDARSLPIAVVESHLLQAQKMGCAVDTDRAGLSTSIMNTIRKNTAGSDLCDFKAVRSRVPEDISNFLLRLAIYELQKEASSTTRHEISWIEPLDKSTTATRLAEKPVCEERQQEPDAIDTSEDDIFISIPMPQEEPCLGSDVPLKSHEPVPQSSGMELASWNQDNLDKDTQDLFSDSPVKTVSQPAKRKLPDWTAGPTTSVTTTSEKRTKKKKGLFS

Target ORF information:

RefSeq Version	XM_009303971.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Werner syndrome (wrn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303971.3

ORF Insert Sequence:

ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA 
GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT 
TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG 
AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT 
CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC 
ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC 
ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT 
GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC 
AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT 
GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT 
GGTTGCTCAG GATCCTCAGC ACCCAGTTTG AAAGAGAGAG TGTTAAAAAT TGCTTGTGAT 
CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA 
CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC 
AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC 
AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA 
AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC CGAGTACGAG 
CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT 
TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG 
TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG 
CCTCAGTCTG GTGCCGAAGA CGAAGATGAA GATGTTGAGG AGAACGAGGA AGAAGGGCTA 
GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA 
CATCACAGTT TCAAACCTGT TCAGTGGAAA GTGATTCAGT CTATTCTCAC AGAGAGGAGA 
GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT 
GTTTACTGCC AGACAATCAG TGTGGTCATT TCACCACTCA TCGCCCTCAT GGAGGATCAA 
GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGATCTGC TCAGACCAAA 
AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC 
TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT 
GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG 
GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC 
GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT 
ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC 
GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG 
GGCTCTGCCA TTGTGTACTG TCCATCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA 
TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA 
GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT 
GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA 
ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT 
CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA 
AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT 
TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA 
GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT 
GAAGAGGATC TAGAGGTGCT GCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG 
GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CCATCCTCTT CCTGCGTGGT 
TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG 
GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG 
ATGGAAGTCA CAGGCCACAG CAAGTTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA 
TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA 
GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT 
ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA 
AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA 
GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA 
GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT 
AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA 
AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA 
GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA 
CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCTG TCGTGAATCA 
GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTCTTC 
CAGACAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATAGCGGTG 
GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA 
GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT 
GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG 
GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT 
GAGCCACTGG ACAAATCAAC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG 
CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC 
ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTACCTC TGAAGTCACA TGAGCCAGTT 
CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA 
CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG 
CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA 
AAGAAGAAAG GACTTTTCAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_009304279.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302554.1 Danio rerio Werner syndrome (wrn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45771
Clone ID Related Accession (Same CDS sequence)	XM_009304279.2
Accession Version	XM_009304279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTAG AGGATGAGAA CATCATGAAA GTTGGCGTGG GAATTGAGGG TGACAAATGG 
AAGTTATTGT CTGATTATGA CATCAAGTTA AAGAATATCG TTGATCTCTC TGATCTGGCA 
AATGAGAAGC TGAGATGTTG TGAGAAGTGG AGTTTGGATG GCCTTGTGAA GCACTTGTTG 
AAGAAGCAGC TCTTTAAGGA CAAACTGGTA CGCTGTAGTC ACTGGGATGA TTTTAGTCTG 
ACAGAAGACC AGAAGAGATA TGCTGCAACA GATGCTTACG CTGGATTGCT CATTTATCAG 
AAACTACAGG AAATGATATC TGGTTGCTCA GGATCCTCAG CGCTCAGTTT GAAAGAGAGA 
GTGTTTAAAA TTGCTTGTGA TCTAAAAGAT CTGGCTGATC ATATTCCCAA TGAACTTGAC 
TGCACAGACG GTGCTGGGAG ACTAGTGGAT GATCTGTCTC AAACTCTTAC ATCTCTAAGA 
TGTGTTTTGT CAAATGCCAA CAAGCCTACT GAACAATTAG TTGAAGAAAA GCCTTCTCCA 
GGACTTATTC TCGACGACAC CAGTTCTCAC CATGAGGGCG TCCCCACAAC TGAAGAGCAG 
AGCCACAGTC AGTGTGTGGA AAACCCTGGC AGACCTCCCC AGAGAGAGTG TGCGATGTCT 
CTGGATATCT CAGAGTACGA GCTGCAGATG CTGGAGGAAC AGGCCAGACA GGAGCAGCTG 
GAGGAGGAGT GTATCTTAGC TTTCCAGACT CAACACGCAT CGGGTCCCCA GGATTTAAAT 
GCGACAGAGA GCGATGAAGA GTTTGATGAT GAAATGATGA AGTGTGCAGA TGACATAGAA 
AAAATAAACA ATGCACAGAT GCCTCAGTCT GGTGCAGAGG ACGAAGATGA AGATGTTGAG 
GAGGACGAGG AAGAAGGGCT AGATTCAAGC ATTCCACAGC CAGTTCCTGA ACAAATCAAA 
TGTCTGAAAA TGTTCTTTGG ACATCACAGT TTCAAACCGG TTCAGTGGAA AGTGATTCAG 
TCCATTCTCA CAGAGAGGAG AGATAATCTT GTCGTCATGG CAACAGGTTA TGGGAAGAGT 
TTGTGCTTCC AGTTTCCTCC TGTGTACTGC CAGACAATCA GTGTGGTCAT TTCACCTCTC 
ATCGCCCTCA TGGAGGATCA AGTGCTTCAG CTCACGATGT CAAACATTCC TGCTTGTTTT 
CTTGGTTCTG CTCAGACCAA AAACCTAAAT GAGCAAGTGA AGAGGGGGGA TTTCAGAGTG 
GTGTACATGA CCCCTGAGTT CTGCTCGGGG AACATTCCTC TGCTAGTGCA GCTCGACAGG 
ACTGTTGGCT TATCACTGAT TGCGGTTGAT GAGGCTCATT GCATTTCTCA GTGGGGTCAT 
GATTTCAGAA GTGCGTATCG GGAGTTGGGC AAACTCAAGA AAAATCTCCC CACTGTCCCT 
TTTTTGGCAC TCACGGCTAC CGCAAGTCCA TCTATCCGTG ACGACATCAT TAAGAGTCTT 
CATCTGATCA ATCCCATGAT TACCTGTACT TCATTTGACC GACCAAACCT TTACTTGGAT 
GTCAATCGCA AGTCCGGGGA CGTCATCCAA GACCTCAAAC GATTTCTTGT TAAAAAGAAA 
GGGGGTGGTT ATGAGTTTGA GGGCTCTGCC ATTGTGTACT GTCCCTCAAA GAAAGAGGCA 
GAGCGTGTGA CAACAGCACT ATTCAAGCTG GACATCCCAT GTGGAGTTTA TCACGCTGGT 
CTGAGCATCA AACAGAGGAG AGAGACTCAG CACCAGTTCA TGAGGGATGA GATTCAGTGT 
GTGGTGGCCA CAGTGGCGTT TGGGATGGGC ATCAATAAAT CTGACATCAG GAAGGTCATC 
CATTATGGGG CTCCCAAAGA AATGGAGTCC TACTATCAGG AGATTGGCAG GGCTGGTAGA 
GACGGGCTGC CCAGTGCCTG TCACGTCTTA TGGATGCCCG GAGATATGGC GCTGAATAAA 
TTCATTCTCA ACCAATCCAA AAGTGAGCGC TTCAGGAGTT ACAAAATAGA CATGATGGCA 
AAAATGGAAA AGTATCTGAA TTCAACCAAA TGTAGAAGAA AGTTGATTTT GTGTCATTTT 
GAAGACAAGC GTCTCAGGAA AGTGACGTCT GGAATTCTGG GAAGCAGTCA ATGTTGCGAT 
AACTGCCGGT CAGGTGTGAA TGAAGAGGAT CTAGAGGTGC TCCAGCAGGA CTTTGGCTTG 
TGTGCATATC AGTTGATGAA GGCCATCAGT GTTATGGAGG AGAGGTTTGG CATCACAGCG 
CCGATCCTCT TCCTGCGTGG TTCATGTTCT CAGCGGGTTC CAGAACGCTT TCGGAAGCAC 
TCTCTCTTTG GTATTGGCAG GGACGTCTCA GAAACTTGGT GGAAAGCTCT TGGCCGAGAA 
CTTATTGCTG AGAAATATCT GATGGAAGTC ACAGGCCACA GCAAATTCAC CACTCTCTGT 
AAAATCACAA CTAAGGGAAG ATCATGGCTG AGTAAAGCTG AAGATGAGAA GCACAGACAG 
CTTTTGCTGC AGCCCAACAG AGATCTGTTC AGCAGAGTGT GTGCTCCGCG GAGAAAAGCA 
GATCCAGCAC AAGTTGAACC TACAGTTTCT AACCTGCAGT CACCTCACAT ACGGCCCTCT 
CTTGTTACAT TTCAGAGGAC AAAGCTTGGT TCTGAATCCC CTCAGATTGT GAAGAAAGGA 
GATTTGCCTT CTAGGTCAGC AGCCAGACAA ACACAGATTC AAACTGATGT CAGAGCCCAA 
ACTCCAGGAG CCTCTGCTAA AGAAATTCAG CTACAGTCTG AGCTGTATGG TAAGCTGGTG 
TCTGAGCGTC AGAAGTTAGC TAGTATCAGA GACATCCCAC CAGCTCTGCT GGCCACCAAT 
AAAATTCTTC TGGACATGTC AAAATTAAGG CCCTGCACGA TGTCCAGCTT GAAACAGGTG 
GATGGAGTTT CAGAAGCAAA AGCAGCCATG TTAACACCTC TCATAGATAT CATCGCCAAA 
TTCTGCCAGA CACACGGATT ACAGGTGGAT GTTTCCTCCA ACTCTGCTTC ATCTGTCCAA 
ACACACTCCA GCCGTGAATC AGTTGTAGGA TCTGTGGCTC AGTGTCTGCC GGACAGCATC 
TCCATCACAT ACAGCCTTTT CCAGATAGAG CACAAGAGCT TGAGGCAGGT ATCAGATGCT 
CGCTCTCTGC CCATTGCGGT GGTGGAGTCT CATCTTCTGC AAGCTCAGAA AATGGGCTGC 
GCTGTGGACA CTGACAGAGC AGGACTCTCA ACATCCATCA TGAACACCAT CAGAAAAAAC 
ACAGCCGGCT CAGATTTGTG TGATTTTAAA GCAGTCCGTT CTCGTGTCCC TGAAGATATT 
AGTAACTTTC TCCTGAGGTT GGCCATTTAT GAACTCCAGA AAGAAGCTTC ATCCACAACA 
CGTCATGAAA TCAGCTGGAT TGAGCCACTG GACAAATCAT CTACTGCAAC CAGACTAGCA 
GAAAAACCTG TGTGTGAAGA GCGACAGCAA GAGCCAGACG CCATAGACAC CAGTGAAGAT 
GATATCTTTA TCAGTATTCC CATGCCTCAG GAAGAACCCT GCTTGGGGAG TGATGTGCCT 
CTGAAGTCAC ATGAGCCAGT TCCTCAGAGC AGTGGAATGG AGCTGGCTTC CTGGAACCAG 
GATAACCTGG ATAAGGACAC ACAAGATCTC TTCAGCGATT CCCCTGTGAA GACTGTGAGT 
CAGCCTGCAA AGAGAAAACT GCCTGACTGG ACTGCAGGAC CTACAACATC AGTAACGACT 
ACATCGGAAA AGAGAACCAA AAAGAAGAAA GGACTTTTCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302554.1
CDS	59..3883
Translation	MFLEDENIMKVGVGIEGDKWKLLSDYDIKLKNIVDLSDLANEKLRCCEKWSLDGLVKHLLKKQLFKDKLVRCSHWDDFSLTEDQKRYAATDAYAGLLIYQKLQEMISGCSGSSALSLKERVFKIACDLKDLADHIPNELDCTDGAGRLVDDLSQTLTSLRCVLSNANKPTEQLVEEKPSPGLILDDTSSHHEGVPTTEEQSHSQCVENPGRPPQRECAMSLDISEYELQMLEEQARQEQLEEECILAFQTQHASGPQDLNATESDEEFDDEMMKCADDIEKINNAQMPQSGAEDEDEDVEEDEEEGLDSSIPQPVPEQIKCLKMFFGHHSFKPVQWKVIQSILTERRDNLVVMATGYGKSLCFQFPPVYCQTISVVISPLIALMEDQVLQLTMSNIPACFLGSAQTKNLNEQVKRGDFRVVYMTPEFCSGNIPLLVQLDRTVGLSLIAVDEAHCISQWGHDFRSAYRELGKLKKNLPTVPFLALTATASPSIRDDIIKSLHLINPMITCTSFDRPNLYLDVNRKSGDVIQDLKRFLVKKKGGGYEFEGSAIVYCPSKKEAERVTTALFKLDIPCGVYHAGLSIKQRRETQHQFMRDEIQCVVATVAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSACHVLWMPGDMALNKFILNQSKSERFRSYKIDMMAKMEKYLNSTKCRRKLILCHFEDKRLRKVTSGILGSSQCCDNCRSGVNEEDLEVLQQDFGLCAYQLMKAISVMEERFGITAPILFLRGSCSQRVPERFRKHSLFGIGRDVSETWWKALGRELIAEKYLMEVTGHSKFTTLCKITTKGRSWLSKAEDEKHRQLLLQPNRDLFSRVCAPRRKADPAQVEPTVSNLQSPHIRPSLVTFQRTKLGSESPQIVKKGDLPSRSAARQTQIQTDVRAQTPGASAKEIQLQSELYGKLVSERQKLASIRDIPPALLATNKILLDMSKLRPCTMSSLKQVDGVSEAKAAMLTPLIDIIAKFCQTHGLQVDVSSNSASSVQTHSSRESVVGSVAQCLPDSISITYSLFQIEHKSLRQVSDARSLPIAVVESHLLQAQKMGCAVDTDRAGLSTSIMNTIRKNTAGSDLCDFKAVRSRVPEDISNFLLRLAIYELQKEASSTTRHEISWIEPLDKSSTATRLAEKPVCEERQQEPDAIDTSEDDIFISIPMPQEEPCLGSDVPLKSHEPVPQSSGMELASWNQDNLDKDTQDLFSDSPVKTVSQPAKRKLPDWTAGPTTSVTTTSEKRTKKKKGLFS

Target ORF information:

RefSeq Version	XM_009304279.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Werner syndrome (wrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304279.2

ORF Insert Sequence:

ATGTTTCTAG AGGATGAGAA CATCATGAAA GTTGGCGTGG GAATTGAGGG TGACAAATGG 
AAGTTATTGT CTGATTATGA CATCAAGTTA AAGAATATCG TTGATCTCTC TGATCTGGCA 
AATGAGAAGC TGAGATGTTG TGAGAAGTGG AGTTTGGATG GCCTTGTGAA GCACTTGTTG 
AAGAAGCAGC TCTTTAAGGA CAAACTGGTA CGCTGTAGTC ACTGGGATGA TTTTAGTCTG 
ACAGAAGACC AGAAGAGATA TGCTGCAACA GATGCTTACG CTGGATTGCT CATTTATCAG 
AAACTACAGG AAATGATATC TGGTTGCTCA GGATCCTCAG CGCTCAGTTT GAAAGAGAGA 
GTGTTTAAAA TTGCTTGTGA TCTAAAAGAT CTGGCTGATC ATATTCCCAA TGAACTTGAC 
TGCACAGACG GTGCTGGGAG ACTAGTGGAT GATCTGTCTC AAACTCTTAC ATCTCTAAGA 
TGTGTTTTGT CAAATGCCAA CAAGCCTACT GAACAATTAG TTGAAGAAAA GCCTTCTCCA 
GGACTTATTC TCGACGACAC CAGTTCTCAC CATGAGGGCG TCCCCACAAC TGAAGAGCAG 
AGCCACAGTC AGTGTGTGGA AAACCCTGGC AGACCTCCCC AGAGAGAGTG TGCGATGTCT 
CTGGATATCT CAGAGTACGA GCTGCAGATG CTGGAGGAAC AGGCCAGACA GGAGCAGCTG 
GAGGAGGAGT GTATCTTAGC TTTCCAGACT CAACACGCAT CGGGTCCCCA GGATTTAAAT 
GCGACAGAGA GCGATGAAGA GTTTGATGAT GAAATGATGA AGTGTGCAGA TGACATAGAA 
AAAATAAACA ATGCACAGAT GCCTCAGTCT GGTGCAGAGG ACGAAGATGA AGATGTTGAG 
GAGGACGAGG AAGAAGGGCT AGATTCAAGC ATTCCACAGC CAGTTCCTGA ACAAATCAAA 
TGTCTGAAAA TGTTCTTTGG ACATCACAGT TTCAAACCGG TTCAGTGGAA AGTGATTCAG 
TCCATTCTCA CAGAGAGGAG AGATAATCTT GTCGTCATGG CAACAGGTTA TGGGAAGAGT 
TTGTGCTTCC AGTTTCCTCC TGTGTACTGC CAGACAATCA GTGTGGTCAT TTCACCTCTC 
ATCGCCCTCA TGGAGGATCA AGTGCTTCAG CTCACGATGT CAAACATTCC TGCTTGTTTT 
CTTGGTTCTG CTCAGACCAA AAACCTAAAT GAGCAAGTGA AGAGGGGGGA TTTCAGAGTG 
GTGTACATGA CCCCTGAGTT CTGCTCGGGG AACATTCCTC TGCTAGTGCA GCTCGACAGG 
ACTGTTGGCT TATCACTGAT TGCGGTTGAT GAGGCTCATT GCATTTCTCA GTGGGGTCAT 
GATTTCAGAA GTGCGTATCG GGAGTTGGGC AAACTCAAGA AAAATCTCCC CACTGTCCCT 
TTTTTGGCAC TCACGGCTAC CGCAAGTCCA TCTATCCGTG ACGACATCAT TAAGAGTCTT 
CATCTGATCA ATCCCATGAT TACCTGTACT TCATTTGACC GACCAAACCT TTACTTGGAT 
GTCAATCGCA AGTCCGGGGA CGTCATCCAA GACCTCAAAC GATTTCTTGT TAAAAAGAAA 
GGGGGTGGTT ATGAGTTTGA GGGCTCTGCC ATTGTGTACT GTCCCTCAAA GAAAGAGGCA 
GAGCGTGTGA CAACAGCACT ATTCAAGCTG GACATCCCAT GTGGAGTTTA TCACGCTGGT 
CTGAGCATCA AACAGAGGAG AGAGACTCAG CACCAGTTCA TGAGGGATGA GATTCAGTGT 
GTGGTGGCCA CAGTGGCGTT TGGGATGGGC ATCAATAAAT CTGACATCAG GAAGGTCATC 
CATTATGGGG CTCCCAAAGA AATGGAGTCC TACTATCAGG AGATTGGCAG GGCTGGTAGA 
GACGGGCTGC CCAGTGCCTG TCACGTCTTA TGGATGCCCG GAGATATGGC GCTGAATAAA 
TTCATTCTCA ACCAATCCAA AAGTGAGCGC TTCAGGAGTT ACAAAATAGA CATGATGGCA 
AAAATGGAAA AGTATCTGAA TTCAACCAAA TGTAGAAGAA AGTTGATTTT GTGTCATTTT 
GAAGACAAGC GTCTCAGGAA AGTGACGTCT GGAATTCTGG GAAGCAGTCA ATGTTGCGAT 
AACTGCCGGT CAGGTGTGAA TGAAGAGGAT CTAGAGGTGC TCCAGCAGGA CTTTGGCTTG 
TGTGCATATC AGTTGATGAA GGCCATCAGT GTTATGGAGG AGAGGTTTGG CATCACAGCG 
CCGATCCTCT TCCTGCGTGG TTCATGTTCT CAGCGGGTTC CAGAACGCTT TCGGAAGCAC 
TCTCTCTTTG GTATTGGCAG GGACGTCTCA GAAACTTGGT GGAAAGCTCT TGGCCGAGAA 
CTTATTGCTG AGAAATATCT GATGGAAGTC ACAGGCCACA GCAAATTCAC CACTCTCTGT 
AAAATCACAA CTAAGGGAAG ATCATGGCTG AGTAAAGCTG AAGATGAGAA GCACAGACAG 
CTTTTGCTGC AGCCCAACAG AGATCTGTTC AGCAGAGTGT GTGCTCCGCG GAGAAAAGCA 
GATCCAGCAC AAGTTGAACC TACAGTTTCT AACCTGCAGT CACCTCACAT ACGGCCCTCT 
CTTGTTACAT TTCAGAGGAC AAAGCTTGGT TCTGAATCCC CTCAGATTGT GAAGAAAGGA 
GATTTGCCTT CTAGGTCAGC AGCCAGACAA ACACAGATTC AAACTGATGT CAGAGCCCAA 
ACTCCAGGAG CCTCTGCTAA AGAAATTCAG CTACAGTCTG AGCTGTATGG TAAGCTGGTG 
TCTGAGCGTC AGAAGTTAGC TAGTATCAGA GACATCCCAC CAGCTCTGCT GGCCACCAAT 
AAAATTCTTC TGGACATGTC AAAATTAAGG CCCTGCACGA TGTCCAGCTT GAAACAGGTG 
GATGGAGTTT CAGAAGCAAA AGCAGCCATG TTAACACCTC TCATAGATAT CATCGCCAAA 
TTCTGCCAGA CACACGGATT ACAGGTGGAT GTTTCCTCCA ACTCTGCTTC ATCTGTCCAA 
ACACACTCCA GCCGTGAATC AGTTGTAGGA TCTGTGGCTC AGTGTCTGCC GGACAGCATC 
TCCATCACAT ACAGCCTTTT CCAGATAGAG CACAAGAGCT TGAGGCAGGT ATCAGATGCT 
CGCTCTCTGC CCATTGCGGT GGTGGAGTCT CATCTTCTGC AAGCTCAGAA AATGGGCTGC 
GCTGTGGACA CTGACAGAGC AGGACTCTCA ACATCCATCA TGAACACCAT CAGAAAAAAC 
ACAGCCGGCT CAGATTTGTG TGATTTTAAA GCAGTCCGTT CTCGTGTCCC TGAAGATATT 
AGTAACTTTC TCCTGAGGTT GGCCATTTAT GAACTCCAGA AAGAAGCTTC ATCCACAACA 
CGTCATGAAA TCAGCTGGAT TGAGCCACTG GACAAATCAT CTACTGCAAC CAGACTAGCA 
GAAAAACCTG TGTGTGAAGA GCGACAGCAA GAGCCAGACG CCATAGACAC CAGTGAAGAT 
GATATCTTTA TCAGTATTCC CATGCCTCAG GAAGAACCCT GCTTGGGGAG TGATGTGCCT 
CTGAAGTCAC ATGAGCCAGT TCCTCAGAGC AGTGGAATGG AGCTGGCTTC CTGGAACCAG 
GATAACCTGG ATAAGGACAC ACAAGATCTC TTCAGCGATT CCCCTGTGAA GACTGTGAGT 
CAGCCTGCAA AGAGAAAACT GCCTGACTGG ACTGCAGGAC CTACAACATC AGTAACGACT 
ACATCGGAAA AGAGAACCAA AAAGAAGAAA GGACTTTTCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_009303971.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302246.1 Danio rerio Werner syndrome (wrn), transcript variant X1, mRNA.

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CloneID	ODa45770
Clone ID Related Accession (Same CDS sequence)	XM_009303971.2 , XM_021479248.1
Accession Version	XM_009303971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA 
GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT 
TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG 
AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT 
CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC 
ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC 
ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT 
GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC 
AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT 
GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT 
GGTTGCTCAG GATCCTCAGC GCTCAGTTTG AAAGAGAGAG TGTTTAAAAT TGCTTGTGAT 
CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA 
CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC 
AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC 
AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA 
AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC AGAGTACGAG 
CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT 
TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG 
TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG 
CCTCAGTCTG GTGCAGAGGA CGAAGATGAA GATGTTGAGG AGGACGAGGA AGAAGGGCTA 
GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA 
CATCACAGTT TCAAACCGGT TCAGTGGAAA GTGATTCAGT CCATTCTCAC AGAGAGGAGA 
GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT 
GTGTACTGCC AGACAATCAG TGTGGTCATT TCACCTCTCA TCGCCCTCAT GGAGGATCAA 
GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGTTCTGC TCAGACCAAA 
AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC 
TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT 
GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG 
GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC 
GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT 
ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC 
GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG 
GGCTCTGCCA TTGTGTACTG TCCCTCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA 
TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA 
GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT 
GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA 
ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT 
CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA 
AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT 
TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA 
GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT 
GAAGAGGATC TAGAGGTGCT CCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG 
GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CGATCCTCTT CCTGCGTGGT 
TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG 
GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG 
ATGGAAGTCA CAGGCCACAG CAAATTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA 
TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA 
GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT 
ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA 
AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA 
GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA 
GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT 
AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA 
AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA 
GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA 
CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCAG CCGTGAATCA 
GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTTTTC 
CAGATAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATTGCGGTG 
GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA 
GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT 
GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG 
GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT 
GAGCCACTGG ACAAATCATC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG 
CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC 
ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTGCCTC TGAAGTCACA TGAGCCAGTT 
CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA 
CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG 
CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA 
AAGAAGAAAG GACTTTTCAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302246.1
CDS	332..4495
Translation	MGDRTLPKWMNNKPQDEHTSGTYKKNVLEDHLPYLEFGGTVVYSQERHDCSFLSEDLRSALSSGSAVGFDLEWPPSFTKGKTKKVAMVQLCASEDKCYLFHISSMSGFPPGLKMFLEDENIMKVGVGIEGDKWKLLSDYDIKLKNIVDLSDLANEKLRCCEKWSLDGLVKHLLKKQLFKDKLVRCSHWDDFSLTEDQKRYAATDAYAGLLIYQKLQEMISGCSGSSALSLKERVFKIACDLKDLADHIPNELDCTDGAGRLVDDLSQTLTSLRCVLSNANKPTEQLVEEKPSPGLILDDTSSHHEGVPTTEEQSHSQCVENPGRPPQRECAMSLDISEYELQMLEEQARQEQLEEECILAFQTQHASGPQDLNATESDEEFDDEMMKCADDIEKINNAQMPQSGAEDEDEDVEEDEEEGLDSSIPQPVPEQIKCLKMFFGHHSFKPVQWKVIQSILTERRDNLVVMATGYGKSLCFQFPPVYCQTISVVISPLIALMEDQVLQLTMSNIPACFLGSAQTKNLNEQVKRGDFRVVYMTPEFCSGNIPLLVQLDRTVGLSLIAVDEAHCISQWGHDFRSAYRELGKLKKNLPTVPFLALTATASPSIRDDIIKSLHLINPMITCTSFDRPNLYLDVNRKSGDVIQDLKRFLVKKKGGGYEFEGSAIVYCPSKKEAERVTTALFKLDIPCGVYHAGLSIKQRRETQHQFMRDEIQCVVATVAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSACHVLWMPGDMALNKFILNQSKSERFRSYKIDMMAKMEKYLNSTKCRRKLILCHFEDKRLRKVTSGILGSSQCCDNCRSGVNEEDLEVLQQDFGLCAYQLMKAISVMEERFGITAPILFLRGSCSQRVPERFRKHSLFGIGRDVSETWWKALGRELIAEKYLMEVTGHSKFTTLCKITTKGRSWLSKAEDEKHRQLLLQPNRDLFSRVCAPRRKADPAQVEPTVSNLQSPHIRPSLVTFQRTKLGSESPQIVKKGDLPSRSAARQTQIQTDVRAQTPGASAKEIQLQSELYGKLVSERQKLASIRDIPPALLATNKILLDMSKLRPCTMSSLKQVDGVSEAKAAMLTPLIDIIAKFCQTHGLQVDVSSNSASSVQTHSSRESVVGSVAQCLPDSISITYSLFQIEHKSLRQVSDARSLPIAVVESHLLQAQKMGCAVDTDRAGLSTSIMNTIRKNTAGSDLCDFKAVRSRVPEDISNFLLRLAIYELQKEASSTTRHEISWIEPLDKSSTATRLAEKPVCEERQQEPDAIDTSEDDIFISIPMPQEEPCLGSDVPLKSHEPVPQSSGMELASWNQDNLDKDTQDLFSDSPVKTVSQPAKRKLPDWTAGPTTSVTTTSEKRTKKKKGLFS

Target ORF information:

RefSeq Version	XM_009303971.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Werner syndrome (wrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303971.2

ORF Insert Sequence:

ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA 
GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT 
TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG 
AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT 
CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC 
ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC 
ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT 
GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC 
AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT 
GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT 
GGTTGCTCAG GATCCTCAGC GCTCAGTTTG AAAGAGAGAG TGTTTAAAAT TGCTTGTGAT 
CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA 
CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC 
AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC 
AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA 
AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC AGAGTACGAG 
CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT 
TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG 
TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG 
CCTCAGTCTG GTGCAGAGGA CGAAGATGAA GATGTTGAGG AGGACGAGGA AGAAGGGCTA 
GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA 
CATCACAGTT TCAAACCGGT TCAGTGGAAA GTGATTCAGT CCATTCTCAC AGAGAGGAGA 
GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT 
GTGTACTGCC AGACAATCAG TGTGGTCATT TCACCTCTCA TCGCCCTCAT GGAGGATCAA 
GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGTTCTGC TCAGACCAAA 
AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC 
TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT 
GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG 
GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC 
GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT 
ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC 
GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG 
GGCTCTGCCA TTGTGTACTG TCCCTCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA 
TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA 
GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT 
GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA 
ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT 
CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA 
AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT 
TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA 
GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT 
GAAGAGGATC TAGAGGTGCT CCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG 
GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CGATCCTCTT CCTGCGTGGT 
TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG 
GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG 
ATGGAAGTCA CAGGCCACAG CAAATTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA 
TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA 
GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT 
ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA 
AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA 
GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA 
GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT 
AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA 
AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA 
GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA 
CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCAG CCGTGAATCA 
GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTTTTC 
CAGATAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATTGCGGTG 
GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA 
GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT 
GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG 
GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT 
GAGCCACTGG ACAAATCATC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG 
CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC 
ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTGCCTC TGAAGTCACA TGAGCCAGTT 
CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA 
CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG 
CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA 
AAGAAGAAAG GACTTTTCAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wrn ( XM_021479248.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334923.1 Danio rerio Werner syndrome (wrn), mRNA.

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CloneID	ODa45770
Clone ID Related Accession (Same CDS sequence)	XM_009303971.2 , XM_021479248.1
Accession Version	XM_021479248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Werner syndrome ATP-dependent helicase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA 
GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT 
TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG 
AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT 
CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC 
ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC 
ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT 
GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC 
AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT 
GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT 
GGTTGCTCAG GATCCTCAGC GCTCAGTTTG AAAGAGAGAG TGTTTAAAAT TGCTTGTGAT 
CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA 
CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC 
AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC 
AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA 
AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC AGAGTACGAG 
CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT 
TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG 
TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG 
CCTCAGTCTG GTGCAGAGGA CGAAGATGAA GATGTTGAGG AGGACGAGGA AGAAGGGCTA 
GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA 
CATCACAGTT TCAAACCGGT TCAGTGGAAA GTGATTCAGT CCATTCTCAC AGAGAGGAGA 
GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT 
GTGTACTGCC AGACAATCAG TGTGGTCATT TCACCTCTCA TCGCCCTCAT GGAGGATCAA 
GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGTTCTGC TCAGACCAAA 
AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC 
TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT 
GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG 
GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC 
GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT 
ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC 
GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG 
GGCTCTGCCA TTGTGTACTG TCCCTCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA 
TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA 
GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT 
GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA 
ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT 
CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA 
AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT 
TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA 
GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT 
GAAGAGGATC TAGAGGTGCT CCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG 
GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CGATCCTCTT CCTGCGTGGT 
TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG 
GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG 
ATGGAAGTCA CAGGCCACAG CAAATTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA 
TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA 
GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT 
ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA 
AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA 
GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA 
GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT 
AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA 
AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA 
GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA 
CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCAG CCGTGAATCA 
GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTTTTC 
CAGATAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATTGCGGTG 
GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA 
GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT 
GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG 
GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT 
GAGCCACTGG ACAAATCATC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG 
CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC 
ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTGCCTC TGAAGTCACA TGAGCCAGTT 
CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA 
CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG 
CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA 
AAGAAGAAAG GACTTTTCAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334923.1
CDS	249..4412
Translation	MGDRTLPKWMNNKPQDEHTSGTYKKNVLEDHLPYLEFGGTVVYSQERHDCSFLSEDLRSALSSGSAVGFDLEWPPSFTKGKTKKVAMVQLCASEDKCYLFHISSMSGFPPGLKMFLEDENIMKVGVGIEGDKWKLLSDYDIKLKNIVDLSDLANEKLRCCEKWSLDGLVKHLLKKQLFKDKLVRCSHWDDFSLTEDQKRYAATDAYAGLLIYQKLQEMISGCSGSSALSLKERVFKIACDLKDLADHIPNELDCTDGAGRLVDDLSQTLTSLRCVLSNANKPTEQLVEEKPSPGLILDDTSSHHEGVPTTEEQSHSQCVENPGRPPQRECAMSLDISEYELQMLEEQARQEQLEEECILAFQTQHASGPQDLNATESDEEFDDEMMKCADDIEKINNAQMPQSGAEDEDEDVEEDEEEGLDSSIPQPVPEQIKCLKMFFGHHSFKPVQWKVIQSILTERRDNLVVMATGYGKSLCFQFPPVYCQTISVVISPLIALMEDQVLQLTMSNIPACFLGSAQTKNLNEQVKRGDFRVVYMTPEFCSGNIPLLVQLDRTVGLSLIAVDEAHCISQWGHDFRSAYRELGKLKKNLPTVPFLALTATASPSIRDDIIKSLHLINPMITCTSFDRPNLYLDVNRKSGDVIQDLKRFLVKKKGGGYEFEGSAIVYCPSKKEAERVTTALFKLDIPCGVYHAGLSIKQRRETQHQFMRDEIQCVVATVAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLPSACHVLWMPGDMALNKFILNQSKSERFRSYKIDMMAKMEKYLNSTKCRRKLILCHFEDKRLRKVTSGILGSSQCCDNCRSGVNEEDLEVLQQDFGLCAYQLMKAISVMEERFGITAPILFLRGSCSQRVPERFRKHSLFGIGRDVSETWWKALGRELIAEKYLMEVTGHSKFTTLCKITTKGRSWLSKAEDEKHRQLLLQPNRDLFSRVCAPRRKADPAQVEPTVSNLQSPHIRPSLVTFQRTKLGSESPQIVKKGDLPSRSAARQTQIQTDVRAQTPGASAKEIQLQSELYGKLVSERQKLASIRDIPPALLATNKILLDMSKLRPCTMSSLKQVDGVSEAKAAMLTPLIDIIAKFCQTHGLQVDVSSNSASSVQTHSSRESVVGSVAQCLPDSISITYSLFQIEHKSLRQVSDARSLPIAVVESHLLQAQKMGCAVDTDRAGLSTSIMNTIRKNTAGSDLCDFKAVRSRVPEDISNFLLRLAIYELQKEASSTTRHEISWIEPLDKSSTATRLAEKPVCEERQQEPDAIDTSEDDIFISIPMPQEEPCLGSDVPLKSHEPVPQSSGMELASWNQDNLDKDTQDLFSDSPVKTVSQPAKRKLPDWTAGPTTSVTTTSEKRTKKKKGLFS

Target ORF information:

RefSeq Version	XM_021479248.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Werner syndrome (wrn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479248.1

ORF Insert Sequence:

ATGGGTGACA GGACTCTACC TAAATGGATG AATAACAAAC CTCAGGATGA ACACACATCA 
GGAACTTATA AAAAGAATGT TTTGGAGGAT CATCTACCGT ATCTGGAATT TGGTGGAACA 
GTCGTATACA GCCAGGAAAG ACACGACTGT TCTTTTTTAT CAGAGGATTT AAGATCTGCT 
TTGTCGTCTG GCTCAGCGGT GGGATTTGAT CTGGAATGGC CACCAAGCTT CACCAAGGGG 
AAGACAAAGA AAGTGGCAAT GGTGCAGCTG TGTGCATCTG AGGACAAATG CTATTTGTTT 
CACATCTCCT CCATGTCAGG GTTTCCTCCT GGCCTGAAGA TGTTTCTAGA GGATGAGAAC 
ATCATGAAAG TTGGCGTGGG AATTGAGGGT GACAAATGGA AGTTATTGTC TGATTATGAC 
ATCAAGTTAA AGAATATCGT TGATCTCTCT GATCTGGCAA ATGAGAAGCT GAGATGTTGT 
GAGAAGTGGA GTTTGGATGG CCTTGTGAAG CACTTGTTGA AGAAGCAGCT CTTTAAGGAC 
AAACTGGTAC GCTGTAGTCA CTGGGATGAT TTTAGTCTGA CAGAAGACCA GAAGAGATAT 
GCTGCAACAG ATGCTTACGC TGGATTGCTC ATTTATCAGA AACTACAGGA AATGATATCT 
GGTTGCTCAG GATCCTCAGC GCTCAGTTTG AAAGAGAGAG TGTTTAAAAT TGCTTGTGAT 
CTAAAAGATC TGGCTGATCA TATTCCCAAT GAACTTGACT GCACAGACGG TGCTGGGAGA 
CTAGTGGATG ATCTGTCTCA AACTCTTACA TCTCTAAGAT GTGTTTTGTC AAATGCCAAC 
AAGCCTACTG AACAATTAGT TGAAGAAAAG CCTTCTCCAG GACTTATTCT CGACGACACC 
AGTTCTCACC ATGAGGGCGT CCCCACAACT GAAGAGCAGA GCCACAGTCA GTGTGTGGAA 
AACCCTGGCA GACCTCCCCA GAGAGAGTGT GCGATGTCTC TGGATATCTC AGAGTACGAG 
CTGCAGATGC TGGAGGAACA GGCCAGACAG GAGCAGCTGG AGGAGGAGTG TATCTTAGCT 
TTCCAGACTC AACACGCATC GGGTCCCCAG GATTTAAATG CGACAGAGAG CGATGAAGAG 
TTTGATGATG AAATGATGAA GTGTGCAGAT GACATAGAAA AAATAAACAA TGCACAGATG 
CCTCAGTCTG GTGCAGAGGA CGAAGATGAA GATGTTGAGG AGGACGAGGA AGAAGGGCTA 
GATTCAAGCA TTCCACAGCC AGTTCCTGAA CAAATCAAAT GTCTGAAAAT GTTCTTTGGA 
CATCACAGTT TCAAACCGGT TCAGTGGAAA GTGATTCAGT CCATTCTCAC AGAGAGGAGA 
GATAATCTTG TCGTCATGGC AACAGGTTAT GGGAAGAGTT TGTGCTTCCA GTTTCCTCCT 
GTGTACTGCC AGACAATCAG TGTGGTCATT TCACCTCTCA TCGCCCTCAT GGAGGATCAA 
GTGCTTCAGC TCACGATGTC AAACATTCCT GCTTGTTTTC TTGGTTCTGC TCAGACCAAA 
AACCTAAATG AGCAAGTGAA GAGGGGGGAT TTCAGAGTGG TGTACATGAC CCCTGAGTTC 
TGCTCGGGGA ACATTCCTCT GCTAGTGCAG CTCGACAGGA CTGTTGGCTT ATCACTGATT 
GCGGTTGATG AGGCTCATTG CATTTCTCAG TGGGGTCATG ATTTCAGAAG TGCGTATCGG 
GAGTTGGGCA AACTCAAGAA AAATCTCCCC ACTGTCCCTT TTTTGGCACT CACGGCTACC 
GCAAGTCCAT CTATCCGTGA CGACATCATT AAGAGTCTTC ATCTGATCAA TCCCATGATT 
ACCTGTACTT CATTTGACCG ACCAAACCTT TACTTGGATG TCAATCGCAA GTCCGGGGAC 
GTCATCCAAG ACCTCAAACG ATTTCTTGTT AAAAAGAAAG GGGGTGGTTA TGAGTTTGAG 
GGCTCTGCCA TTGTGTACTG TCCCTCAAAG AAAGAGGCAG AGCGTGTGAC AACAGCACTA 
TTCAAGCTGG ACATCCCATG TGGAGTTTAT CACGCTGGTC TGAGCATCAA ACAGAGGAGA 
GAGACTCAGC ACCAGTTCAT GAGGGATGAG ATTCAGTGTG TGGTGGCCAC AGTGGCGTTT 
GGGATGGGCA TCAATAAATC TGACATCAGG AAGGTCATCC ATTATGGGGC TCCCAAAGAA 
ATGGAGTCCT ACTATCAGGA GATTGGCAGG GCTGGTAGAG ACGGGCTGCC CAGTGCCTGT 
CACGTCTTAT GGATGCCCGG AGATATGGCG CTGAATAAAT TCATTCTCAA CCAATCCAAA 
AGTGAGCGCT TCAGGAGTTA CAAAATAGAC ATGATGGCAA AAATGGAAAA GTATCTGAAT 
TCAACCAAAT GTAGAAGAAA GTTGATTTTG TGTCATTTTG AAGACAAGCG TCTCAGGAAA 
GTGACGTCTG GAATTCTGGG AAGCAGTCAA TGTTGCGATA ACTGCCGGTC AGGTGTGAAT 
GAAGAGGATC TAGAGGTGCT CCAGCAGGAC TTTGGCTTGT GTGCATATCA GTTGATGAAG 
GCCATCAGTG TTATGGAGGA GAGGTTTGGC ATCACAGCGC CGATCCTCTT CCTGCGTGGT 
TCATGTTCTC AGCGGGTTCC AGAACGCTTT CGGAAGCACT CTCTCTTTGG TATTGGCAGG 
GACGTCTCAG AAACTTGGTG GAAAGCTCTT GGCCGAGAAC TTATTGCTGA GAAATATCTG 
ATGGAAGTCA CAGGCCACAG CAAATTCACC ACTCTCTGTA AAATCACAAC TAAGGGAAGA 
TCATGGCTGA GTAAAGCTGA AGATGAGAAG CACAGACAGC TTTTGCTGCA GCCCAACAGA 
GATCTGTTCA GCAGAGTGTG TGCTCCGCGG AGAAAAGCAG ATCCAGCACA AGTTGAACCT 
ACAGTTTCTA ACCTGCAGTC ACCTCACATA CGGCCCTCTC TTGTTACATT TCAGAGGACA 
AAGCTTGGTT CTGAATCCCC TCAGATTGTG AAGAAAGGAG ATTTGCCTTC TAGGTCAGCA 
GCCAGACAAA CACAGATTCA AACTGATGTC AGAGCCCAAA CTCCAGGAGC CTCTGCTAAA 
GAAATTCAGC TACAGTCTGA GCTGTATGGT AAGCTGGTGT CTGAGCGTCA GAAGTTAGCT 
AGTATCAGAG ACATCCCACC AGCTCTGCTG GCCACCAATA AAATTCTTCT GGACATGTCA 
AAATTAAGGC CCTGCACGAT GTCCAGCTTG AAACAGGTGG ATGGAGTTTC AGAAGCAAAA 
GCAGCCATGT TAACACCTCT CATAGATATC ATCGCCAAAT TCTGCCAGAC ACACGGATTA 
CAGGTGGATG TTTCCTCCAA CTCTGCTTCA TCTGTCCAAA CACACTCCAG CCGTGAATCA 
GTTGTAGGAT CTGTGGCTCA GTGTCTGCCG GACAGCATCT CCATCACATA CAGCCTTTTC 
CAGATAGAGC ACAAGAGCTT GAGGCAGGTA TCAGATGCTC GCTCTCTGCC CATTGCGGTG 
GTGGAGTCTC ATCTTCTGCA AGCTCAGAAA ATGGGCTGCG CTGTGGACAC TGACAGAGCA 
GGACTCTCAA CATCCATCAT GAACACCATC AGAAAAAACA CAGCCGGCTC AGATTTGTGT 
GATTTTAAAG CAGTCCGTTC TCGTGTCCCT GAAGATATTA GTAACTTTCT CCTGAGGTTG 
GCCATTTATG AACTCCAGAA AGAAGCTTCA TCCACAACAC GTCATGAAAT CAGCTGGATT 
GAGCCACTGG ACAAATCATC TACTGCAACC AGACTAGCAG AAAAACCTGT GTGTGAAGAG 
CGACAGCAAG AGCCAGACGC CATAGACACC AGTGAAGATG ATATCTTTAT CAGTATTCCC 
ATGCCTCAGG AAGAACCCTG CTTGGGGAGT GATGTGCCTC TGAAGTCACA TGAGCCAGTT 
CCTCAGAGCA GTGGAATGGA GCTGGCTTCC TGGAACCAGG ATAACCTGGA TAAGGACACA 
CAAGATCTCT TCAGCGATTC CCCTGTGAAG ACTGTGAGTC AGCCTGCAAA GAGAAAACTG 
CCTGACTGGA CTGCAGGACC TACAACATCA GTAACGACTA CATCGGAAAA GAGAACCAAA 
AAGAAGAAAG GACTTTTCAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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wrn cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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