AGBE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGBE
Entrez Gene ID	326264
Full Name	1,4-Alpha-Glucan Branching Enzyme
Synonyms	CG33138,CG4023,Dmel\CG33138
General protein information	Preferred Names 1,4-Alpha-Glucan Branching Enzyme Names CG33138 gene product from transcript CG33138-RA 1,4-Alpha-Glucan branching enzyme AGBE-PA CG33138-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49F4-49F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_176162.3	NP_788342.1	1,4-Alpha-Glucan branching enzyme

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GBE1	XP_425536.4
Danio rerio (zebrafish)	si:ch211-213e17.1	XP_002663606.3
Canis lupus familiaris (dog)	LOC478380	XP_535555.3
Rattus norvegicus (Norway rat)	Gbe1	NP_001093972.1
Arabidopsis thaliana (thale cress)	SBE2.1	NP_181180.1
Xenopus tropicalis (tropical clawed frog)	gbe1	XP_004912135.1
Homo sapiens (human)	GBE1	NP_000149.3
Magnaporthe oryzae (rice blast fungus)	MGG_03186	XP_003716803.1
Oryza sativa (rice)	Os02g0528200	NP_001047009.1
Bos taurus (cattle)	GBE1	NP_001116201.1
Drosophila melanogaster (fruit fly)	CG33138	NP_001260941.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010428	XP_311519.3
Kluyveromyces lactis	KLLA0A11176g	XP_451486.1
Eremothecium gossypii	AGOS_AEL044W	NP_984817.1
Neurospora crassa	NCU05429	XP_963252.2
Arabidopsis thaliana (thale cress)	SBE2.2	NP_195985.3
Pan troglodytes (chimpanzee)	GBE1	XP_516593.2
	GBE1	XP_001118968.2
Mus musculus (house mouse)	Gbe1	NP_083079.1
Danio rerio (zebrafish)	LOC559212	XP_687620.6
Caenorhabditis elegans (roundworm)	CELE_T04A8.7	NP_497961.1
Saccharomyces cerevisiae (baker's yeast)	GLC3	NP_010905.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGBE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGBE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11762	NM_176162.3 Latest version!	Drosophila melanogaster 1,4-Alpha-Glucan branching enzyme (AGBE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGBE ( NM_176162.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788342.1 Drosophila melanogaster 1,4-Alpha-Glucan branching enzyme (AGBE), mRNA.

Price & Availability↑

CloneID	OFa11762
Clone ID Related Accession (Same CDS sequence)	NM_176162.3
Accession Version	NM_176162.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	1,4-Alpha-Glucan branching enzyme
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_176162.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGG CTAAGGACAT CGAGAAGCTG TTCGAGACGG ACGGCTACTT GCGGCCGTTT 
GAGCATGAGA TCCGTCGCCG TCATGGCGTG CTCGAGGATT GGCTGAATAA GATAAACCAA 
AGCGAGGGCG GACTGGATGG CTTTTCGACG GCTTACAAGC ACTACGGACT TCACTTCCAG 
CCGGATAATT CGGTGATTGC CCGCGAGTGG GCACCTGGAG CCAGAGATGT CTATCTCACG 
GGTGACTTCA ACAACTGGCA CTGGGAATCG CATCCATTTA AAAAGCTCGA CTTTGGCAAG 
TGGGAGCTAC ACCTGCCCCC CAACGAGGAC GGCAGTCCGG CCATCAAGCA CTTGAGCGAA 
ATTAAGATTA TAATTCGCAA CCATTCCGGT CAATTGCTGG ACCGCCTGTC GCCCTGGGCC 
AAGTACGTGG TGCAGCCGCC CAAGTCGGCC AATCAGGGGG TCAACTACAA GCAGTACGTG 
TGGGAGCCAC CGTCCTACGA GCGCTACCAA CGCCAGCATC CGGGTCCGCC AAGACCCAAG 
TCGCTCAGGA TCTACGAGTG CCACGTGGGT ATCGCCTCCC AGGAGCCGCG GGTCGGCAGC 
TACGACGAGT TCGCCGATCG CATCGTGCCG CGCATCAAGC GTCAGGGCTA TAATTGCATC 
CAGGTTATGG CCATCATGGA GCACGCCTAC TACGCCAGTT TTGGCTATCA AGTGACCAGC 
TTCTATGCGG CCTCCAGCCG TTATGGCAAC CCGGAGCAGC TGAAGCGCAT GATCGACGTG 
GCACACTCGC ACGGCCTGTT CGTTCTGCTC GATGTGGTCC ACTCGCATGC CTCCAAGAAC 
GTTCAGGACG GTCTGAACCA GTTCGATGGC ACCAACAGTT GCTTCTTCCA CGACGGAGCC 
CGTGGCGAGC ACTCGCTGTG GGACAGTCGT CTCTTCAACT ACGTGGAGTA CGAGGTGCTG 
CGTTTCTTGC TATCCAACCT GCGTTGGTGG CACGACGAGT ACAACTTCGA TGGCTATCGC 
TTCGACGGAG TGACCTCTAT GCTATACCAT TCCCGTGGCA TTGGGGAGGG CTTCAGCGGC 
GATTACAACG AGTACTTTGG CCTGAATGTC GACACGGATG CCCTCAATTA TCTGGGACTG 
GCCAATCATC TGCTGCACAC CATAGATTCG AGGATTATCA CCATTGCAGA GGATGTTTCT 
GGAATGCCTA CCTTGTGTCG CCCCGTATCA GAGGGTGGTA TCGGTTTCGA CTACCGCTTG 
GGTATGGCCA TCCCAGACAA GTGGATCGAA CTGCTGAAGG AGCAGAGTGA CGATGAGTGG 
GACATGGGCA ACTTGGTGCA CACGCTGACC AACCGTCGCT GGATGGAGAA CACAGTGGCC 
TACGCAGAGT CCCACGATCA AGCGCTAGTG GGCGACAAGA CGATCGCCTT CTGGCTGATG 
GACAAGGAGA TGTATACGCA CATGTCGACG TTGTCGGACT CCTCAGTGAT CATCGACCGA 
GGACTGGCGC TGCACAAGAT GATTCGTCTG ATCACGCACG CCCTGGGAGG CGAGGCGTAC 
CTGAACTTTA TGGGCAACGA GTTCGGACAT CCAGAATGGC TGGACTTCCC ACGCGTTGGC 
AACAACGATT CGTATCACTA TGCTCGCCGG CAATGGAATC TTGTGGACGA CGATCTGCTG 
AAGTACAAGT ACCTCAACGA ATTCGATCGA GCCATGAATG AGGCCGAGGA GCGCTACGGC 
TGGCTGCATT CCGGACCCGC TTGGGTCAGC TGGAAGCACG AGGGCGACAA GATAATCGCT 
TTCGAGCGTG CCGGCCTGGT GTTCGTTTTC AACTTCCATC CACAGCAGAG TTTCACCGGA 
TACCGTGTGG GCACCAACTG GGCGGGCACC TACCAGGCGG TGCTCTCCTC AGACGATCCT 
CTCTTCGGCG GCCACAACCG CATCGATGCC AACTGCAAAC ATCCCTCCAA TCCGGAGGGC 
TACGCCGGGC GCTCCAATTT CATTGAGGTC TACACACCAT CACGCACAGC CGTGGTTTAT 
GCCCGCGTCA GTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788342.1
CDS	120..2177
Translation	MAEAKDIEKLFETDGYLRPFEHEIRRRHGVLEDWLNKINQSEGGLDGFSTAYKHYGLHFQPDNSVIAREWAPGARDVYLTGDFNNWHWESHPFKKLDFGKWELHLPPNEDGSPAIKHLSEIKIIIRNHSGQLLDRLSPWAKYVVQPPKSANQGVNYKQYVWEPPSYERYQRQHPGPPRPKSLRIYECHVGIASQEPRVGSYDEFADRIVPRIKRQGYNCIQVMAIMEHAYYASFGYQVTSFYAASSRYGNPEQLKRMIDVAHSHGLFVLLDVVHSHASKNVQDGLNQFDGTNSCFFHDGARGEHSLWDSRLFNYVEYEVLRFLLSNLRWWHDEYNFDGYRFDGVTSMLYHSRGIGEGFSGDYNEYFGLNVDTDALNYLGLANHLLHTIDSRIITIAEDVSGMPTLCRPVSEGGIGFDYRLGMAIPDKWIELLKEQSDDEWDMGNLVHTLTNRRWMENTVAYAESHDQALVGDKTIAFWLMDKEMYTHMSTLSDSSVIIDRGLALHKMIRLITHALGGEAYLNFMGNEFGHPEWLDFPRVGNNDSYHYARRQWNLVDDDLLKYKYLNEFDRAMNEAEERYGWLHSGPAWVSWKHEGDKIIAFERAGLVFVFNFHPQQSFTGYRVGTNWAGTYQAVLSSDDPLFGGHNRIDANCKHPSNPEGYAGRSNFIEVYTPSRTAVVYARVSD

Target ORF information:

RefSeq Version	NM_176162.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 1,4-Alpha-Glucan branching enzyme (AGBE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176162.3

ORF Insert Sequence:

ATGGCCGAGG CTAAGGACAT CGAGAAGCTG TTCGAGACGG ACGGCTACTT GCGGCCGTTT 
GAGCATGAGA TCCGTCGCCG TCATGGCGTG CTCGAGGATT GGCTGAATAA GATAAACCAA 
AGCGAGGGCG GACTGGATGG CTTTTCGACG GCTTACAAGC ACTACGGACT TCACTTCCAG 
CCGGATAATT CGGTGATTGC CCGCGAGTGG GCACCTGGAG CCAGAGATGT CTATCTCACG 
GGTGACTTCA ACAACTGGCA CTGGGAATCG CATCCATTTA AAAAGCTCGA CTTTGGCAAG 
TGGGAGCTAC ACCTGCCCCC CAACGAGGAC GGCAGTCCGG CCATCAAGCA CTTGAGCGAA 
ATTAAGATTA TAATTCGCAA CCATTCCGGT CAATTGCTGG ACCGCCTGTC GCCCTGGGCC 
AAGTACGTGG TGCAGCCGCC CAAGTCGGCC AATCAGGGGG TCAACTACAA GCAGTACGTG 
TGGGAGCCAC CGTCCTACGA GCGCTACCAA CGCCAGCATC CGGGTCCGCC AAGACCCAAG 
TCGCTCAGGA TCTACGAGTG CCACGTGGGT ATCGCCTCCC AGGAGCCGCG GGTCGGCAGC 
TACGACGAGT TCGCCGATCG CATCGTGCCG CGCATCAAGC GTCAGGGCTA TAATTGCATC 
CAGGTTATGG CCATCATGGA GCACGCCTAC TACGCCAGTT TTGGCTATCA AGTGACCAGC 
TTCTATGCGG CCTCCAGCCG TTATGGCAAC CCGGAGCAGC TGAAGCGCAT GATCGACGTG 
GCACACTCGC ACGGCCTGTT CGTTCTGCTC GATGTGGTCC ACTCGCATGC CTCCAAGAAC 
GTTCAGGACG GTCTGAACCA GTTCGATGGC ACCAACAGTT GCTTCTTCCA CGACGGAGCC 
CGTGGCGAGC ACTCGCTGTG GGACAGTCGT CTCTTCAACT ACGTGGAGTA CGAGGTGCTG 
CGTTTCTTGC TATCCAACCT GCGTTGGTGG CACGACGAGT ACAACTTCGA TGGCTATCGC 
TTCGACGGAG TGACCTCTAT GCTATACCAT TCCCGTGGCA TTGGGGAGGG CTTCAGCGGC 
GATTACAACG AGTACTTTGG CCTGAATGTC GACACGGATG CCCTCAATTA TCTGGGACTG 
GCCAATCATC TGCTGCACAC CATAGATTCG AGGATTATCA CCATTGCAGA GGATGTTTCT 
GGAATGCCTA CCTTGTGTCG CCCCGTATCA GAGGGTGGTA TCGGTTTCGA CTACCGCTTG 
GGTATGGCCA TCCCAGACAA GTGGATCGAA CTGCTGAAGG AGCAGAGTGA CGATGAGTGG 
GACATGGGCA ACTTGGTGCA CACGCTGACC AACCGTCGCT GGATGGAGAA CACAGTGGCC 
TACGCAGAGT CCCACGATCA AGCGCTAGTG GGCGACAAGA CGATCGCCTT CTGGCTGATG 
GACAAGGAGA TGTATACGCA CATGTCGACG TTGTCGGACT CCTCAGTGAT CATCGACCGA 
GGACTGGCGC TGCACAAGAT GATTCGTCTG ATCACGCACG CCCTGGGAGG CGAGGCGTAC 
CTGAACTTTA TGGGCAACGA GTTCGGACAT CCAGAATGGC TGGACTTCCC ACGCGTTGGC 
AACAACGATT CGTATCACTA TGCTCGCCGG CAATGGAATC TTGTGGACGA CGATCTGCTG 
AAGTACAAGT ACCTCAACGA ATTCGATCGA GCCATGAATG AGGCCGAGGA GCGCTACGGC 
TGGCTGCATT CCGGACCCGC TTGGGTCAGC TGGAAGCACG AGGGCGACAA GATAATCGCT 
TTCGAGCGTG CCGGCCTGGT GTTCGTTTTC AACTTCCATC CACAGCAGAG TTTCACCGGA 
TACCGTGTGG GCACCAACTG GGCGGGCACC TACCAGGCGG TGCTCTCCTC AGACGATCCT 
CTCTTCGGCG GCCACAACCG CATCGATGCC AACTGCAAAC ATCCCTCCAA TCCGGAGGGC 
TACGCCGGGC GCTCCAATTT CATTGAGGTC TACACACCAT CACGCACAGC CGTGGTTTAT 
GCCCGCGTCA GTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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AGBE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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