Vm32E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vm32E
Entrez Gene ID	34558
Full Name	Vitelline membrane 32E
Synonyms	CG16874,Dmel\CG16874,VM321,VM32E,VMP32E,Vm3,Vm32Ea,Vm32Ec
General protein information	Preferred Names Vitelline membrane 32E Names CG16874 gene product from transcript CG16874-RB CG16874-PA CG16874-PB Vm32E-PA Vm32E-PB vitelline membrane 32E
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32E1-32E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298908.1	NP_001285837.1	vitelline membrane 32E, isoform B
NM_057755.2	NP_477103.1	vitelline membrane 32E, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

heterodimeric complex EcR-B1/Usp mediates the ecdysone-dependent VM32E transcriptional activation in the main body follicle cells.
Title: EcR-B1 and Usp nuclear hormone receptors regulate expression of the VM32E eggshell gene during Drosophila oogenesis.

the expression of the VM32E gene in the main body follicle cells is modulated by the epidermal growth factor receptor (Egfr) activity
Title: Egfr signaling modulates VM32E gene expression during Drosophila oogenesis.

The following Vm32E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vm32E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16672	NM_057755.2 Latest version!	Drosophila melanogaster vitelline membrane 32E, transcript variant A (Vm32E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa16672	NM_001298908.1 Latest version!	Drosophila melanogaster vitelline membrane 32E, transcript variant B (Vm32E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vm32E ( NM_001298908.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285837.1 Drosophila melanogaster vitelline membrane 32E, transcript variant B (Vm32E), mRNA.

Price & Availability↑

CloneID	OFa16672
Clone ID Related Accession (Same CDS sequence)	NM_001298908.1 , NM_057755.2
Accession Version	NM_001298908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vitelline membrane 32E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGATCG TTGCTCTCAC CCTCGTTGCG TTTGTGGCCA TTGCCGGTGC CTCCTGCCCG 
TATGCAGCTC CAGCTCCAGC TTATTCAGCG CCCGCTGCTT CTTCTGGCTA CCCGGCTCCA 
CCATGCCCCA CCAACTACCT GTTCAGCTGC CAGCCCAATT TGGCCCCAGC TCCTTGTGCC 
CAGGAGGCCC CAGCCTATGG ATCCGCCGGC GCCTACACAG AACAGGTGCC CCACTACGTG 
GGAAGTCCCA ACCGAGAGCA GTTGCAGCAA TTTCACCAGC GCATTGGAAT GGCGGCTTTG 
ATGGAGGAAC TGCGCGGCTT GGGCCAAGGA ATCCAGGGTC AACAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285837.1
CDS	32..382
Translation	MQIVALTLVAFVAIAGASCPYAAPAPAYSAPAASSGYPAPPCPTNYLFSCQPNLAPAPCAQEAPAYGSAGAYTEQVPHYVGSPNREQLQQFHQRIGMAALMEELRGLGQGIQGQQY

Target ORF information:

RefSeq Version	NM_001298908.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vitelline membrane 32E, transcript variant B (Vm32E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298908.1

ORF Insert Sequence:

ATGCAGATCG TTGCTCTCAC CCTCGTTGCG TTTGTGGCCA TTGCCGGTGC CTCCTGCCCG 
TATGCAGCTC CAGCTCCAGC TTATTCAGCG CCCGCTGCTT CTTCTGGCTA CCCGGCTCCA 
CCATGCCCCA CCAACTACCT GTTCAGCTGC CAGCCCAATT TGGCCCCAGC TCCTTGTGCC 
CAGGAGGCCC CAGCCTATGG ATCCGCCGGC GCCTACACAG AACAGGTGCC CCACTACGTG 
GGAAGTCCCA ACCGAGAGCA GTTGCAGCAA TTTCACCAGC GCATTGGAAT GGCGGCTTTG 
ATGGAGGAAC TGCGCGGCTT GGGCCAAGGA ATCCAGGGTC AACAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vm32E ( NM_057755.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477103.1 Drosophila melanogaster vitelline membrane 32E, transcript variant A (Vm32E), mRNA.

Price & Availability↑

CloneID	OFa16672
Clone ID Related Accession (Same CDS sequence)	NM_001298908.1 , NM_057755.2
Accession Version	NM_057755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vitelline membrane 32E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Mar 19, 2002 this sequence version replaced NM_057755.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGATCG TTGCTCTCAC CCTCGTTGCG TTTGTGGCCA TTGCCGGTGC CTCCTGCCCG 
TATGCAGCTC CAGCTCCAGC TTATTCAGCG CCCGCTGCTT CTTCTGGCTA CCCGGCTCCA 
CCATGCCCCA CCAACTACCT GTTCAGCTGC CAGCCCAATT TGGCCCCAGC TCCTTGTGCC 
CAGGAGGCCC CAGCCTATGG ATCCGCCGGC GCCTACACAG AACAGGTGCC CCACTACGTG 
GGAAGTCCCA ACCGAGAGCA GTTGCAGCAA TTTCACCAGC GCATTGGAAT GGCGGCTTTG 
ATGGAGGAAC TGCGCGGCTT GGGCCAAGGA ATCCAGGGTC AACAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477103.1
CDS	32..382
Translation	MQIVALTLVAFVAIAGASCPYAAPAPAYSAPAASSGYPAPPCPTNYLFSCQPNLAPAPCAQEAPAYGSAGAYTEQVPHYVGSPNREQLQQFHQRIGMAALMEELRGLGQGIQGQQY

Target ORF information:

RefSeq Version	NM_057755.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vitelline membrane 32E, transcript variant A (Vm32E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057755.2

ORF Insert Sequence:

ATGCAGATCG TTGCTCTCAC CCTCGTTGCG TTTGTGGCCA TTGCCGGTGC CTCCTGCCCG 
TATGCAGCTC CAGCTCCAGC TTATTCAGCG CCCGCTGCTT CTTCTGGCTA CCCGGCTCCA 
CCATGCCCCA CCAACTACCT GTTCAGCTGC CAGCCCAATT TGGCCCCAGC TCCTTGTGCC 
CAGGAGGCCC CAGCCTATGG ATCCGCCGGC GCCTACACAG AACAGGTGCC CCACTACGTG 
GGAAGTCCCA ACCGAGAGCA GTTGCAGCAA TTTCACCAGC GCATTGGAAT GGCGGCTTTG 
ATGGAGGAAC TGCGCGGCTT GGGCCAAGGA ATCCAGGGTC AACAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vm32E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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