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vari cDNA ORF clone, Drosophila melanogaster(fruit fly)

Summary

Gene Symbol vari
Entrez Gene ID 35343
Full Name varicose
Synonyms 38E.21,CG9326,Dmel\CG9326,Vari,l(2)03953,l(2)03953b,l(2)38EFa,szar
General protein information
Preferred Names

varicose

Names

CG9326 gene product from transcript CG9326-RD
CG9326-PB
CG9326-PC
CG9326-PD
CG9326-PE
CG9326-PF
lethal (2) 38EFa
senz'aria
senz-aria
vari-PB
vari-PC
vari-PD
vari-PE
vari-PF
Gene Type protein-coding
Organism Drosophila melanogaster(fruit fly)
Genome

2L

38E1-39A3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_165342.4 NP_724288.3 varicose, isoform B
NM_165343.3 NP_724289.2 varicose, isoform C
NM_001273715.1 NP_001260644.1 varicose, isoform E
NM_001273716.1 NP_001260645.1 varicose, isoform F
NM_206011.3 NP_995733.2 varicose, isoform D

Pathways from BioSystems


Homologies


MPP6 XP_002803397.1
Canis lupus familiaris (dog) MPP6 XP_005628695.1
Rattus norvegicus (Norway rat) Mpp6 NP_001128454.1
Danio rerio (zebrafish) mpp6a NP_001243118.1
Drosophila melanogaster (fruit fly) vari NP_001260644.1
Xenopus tropicalis (tropical clawed frog) mpp6 NP_001017060.1
Homo sapiens (human) MPP6 NP_057531.2
Pan troglodytes (chimpanzee) MPP6 NP_001233585.1
Mus musculus (house mouse) Mpp6 NP_001158205.1
Danio rerio (zebrafish) mpp6b NP_001038242.1
Bos taurus (cattle) MPP6 XP_005205614.1
Gallus gallus (chicken) MPP6 XP_418721.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP008321 XP_317142.4

Gene Ontology


Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vari fundamentally extends the similarity of Drosophila and vertebrate basolateral regions from sharing only polarity complexes to sharing both polarity and cell-adhesion complexes.
Title: Drosophila Varicose, a member of a new subgroup of basolateral MAGUKs, is required for septate junctions and tracheal morphogenesis.

The following vari gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vari cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa15062 NM_206011.3
Latest version!		 Drosophila melanogaster varicose (vari), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa15063 NM_165343.3
Latest version!		 Drosophila melanogaster varicose (vari), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa15063 NM_001273716.1
Latest version!		 Drosophila melanogaster varicose (vari), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OFa15064 NM_165342.4
Latest version!		 Drosophila melanogaster varicose (vari), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa16954 NM_001273715.1
Latest version!		 Drosophila melanogaster varicose (vari), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OFa15062
Clone ID Related Accession (Same CDS sequence) NM_206011.3
Accession Version NM_206011.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product varicose, isoform D
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_206011.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTTGCTACAT GCGTGCCCTT TTCACATACA ATCCATCCGA AGATTCCTTG 
CTGCCATGCA GGGATATTGG ATTGCCCTTC AAGTCGGGCG ACATTTTGCA GATCATCAAC 
GTAAAGGATC CCAACTGGTG GCAGGCCAAG AACATTACTG CCGAATCTGA CAAAATTGGA 
CTCATACCAT CCCAAGAGCT GGAGGAGCGG CGCAAAGCCT TCGTGGCCCC CGAGGCGGAC 
TACGTTCACA AGATTGGCAT TTGCGGAACA AGAATCTCGA AGCGAAAGCG CAAGACCATG 
TACCGATCTG TGGCCAATTG CGAGTTCGAC AAGGCGGAGC TGCTGCTCTA TGAGGAGGTC 
ACGCGGATGC CGCCGTTCCG CAGGAAAACC CTGGTTCTCA TCGGTGTTTC CGGGGTGGGA 
AGACGTACGC TCAAGAATCG GCTGATCAAC AGCGATGTGG ACAAGTTCGG AGCGGTCATT 
CCACATACCA GTCGCCCCAA GCGCGCCTTG GAGGAGAACG GCAGTAGCTA CTGGTTCATG 
GACCGCGAGG AAATGGAAGA GGCCGTGCGG AACAACGAGT TCCTGGAGTA CGGCGAGCAC 
AATGGCAATC TGTACGGCAC GCATTTGCAG TCCATCAAGG ATGTGATCAA CAGTGGGCGC 
ATGTGCATCC TGGATTGTGC ACCGAATGCC CTGAAGATCC TGCACAACAG CCAGGAACTG 
ATGCCTTTTG TCATCTTTGT GGCAGCGCCT GGCATGGAGC AGCTCAAGAC CATCTATGCG 
GATCGCAGGG CCACGGGCTC CAACCGGAAT TTATCTTTTG ATCGCCAGAG TTCCATAAGA 
TTCAGCTCCA GACGCGCCCG TACGCTCGAG TCCCTAGCGT CGTTGTATGA GGACGACGAC 
CTTGTCGCCA CCGTCGAGGA GAGCAGCTTC GTCCAGCGCA AGTATGAGAA ATACTTCGAC 
ATGGTCATCG TGAACGAGGA CTTCGATGAG ACGTTCCGCC AGGTGGTGGA AACGCTGGAT 
CAGATGAGCC ACGAGGAGCA GTGGGTGCCT GTCAACTGGA TCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_995733.2
CDS285..2132
Translation

Target ORF information:

RefSeq Version NM_206011.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster varicose (vari), transcript variant D, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_206011.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTTGCTACAT GCGTGCCCTT TTCACATACA ATCCATCCGA AGATTCCTTG 
CTGCCATGCA GGGATATTGG ATTGCCCTTC AAGTCGGGCG ACATTTTGCA GATCATCAAC 
GTAAAGGATC CCAACTGGTG GCAGGCCAAG AACATTACTG CCGAATCTGA CAAAATTGGA 
CTCATACCAT CCCAAGAGCT GGAGGAGCGG CGCAAAGCCT TCGTGGCCCC CGAGGCGGAC 
TACGTTCACA AGATTGGCAT TTGCGGAACA AGAATCTCGA AGCGAAAGCG CAAGACCATG 
TACCGATCTG TGGCCAATTG CGAGTTCGAC AAGGCGGAGC TGCTGCTCTA TGAGGAGGTC 
ACGCGGATGC CGCCGTTCCG CAGGAAAACC CTGGTTCTCA TCGGTGTTTC CGGGGTGGGA 
AGACGTACGC TCAAGAATCG GCTGATCAAC AGCGATGTGG ACAAGTTCGG AGCGGTCATT 
CCACATACCA GTCGCCCCAA GCGCGCCTTG GAGGAGAACG GCAGTAGCTA CTGGTTCATG 
GACCGCGAGG AAATGGAAGA GGCCGTGCGG AACAACGAGT TCCTGGAGTA CGGCGAGCAC 
AATGGCAATC TGTACGGCAC GCATTTGCAG TCCATCAAGG ATGTGATCAA CAGTGGGCGC 
ATGTGCATCC TGGATTGTGC ACCGAATGCC CTGAAGATCC TGCACAACAG CCAGGAACTG 
ATGCCTTTTG TCATCTTTGT GGCAGCGCCT GGCATGGAGC AGCTCAAGAC CATCTATGCG 
GATCGCAGGG CCACGGGCTC CAACCGGAAT TTATCTTTTG ATCGCCAGAG TTCCATAAGA 
TTCAGCTCCA GACGCGCCCG TACGCTCGAG TCCCTAGCGT CGTTGTATGA GGACGACGAC 
CTTGTCGCCA CCGTCGAGGA GAGCAGCTTC GTCCAGCGCA AGTATGAGAA ATACTTCGAC 
ATGGTCATCG TGAACGAGGA CTTCGATGAG ACGTTCCGCC AGGTGGTGGA AACGCTGGAT 
CAGATGAGCC ACGAGGAGCA GTGGGTGCCT GTCAACTGGA TCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa15063
Clone ID Related Accession (Same CDS sequence) NM_165343.3 , NM_001273716.1
Accession Version NM_165343.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product varicose, isoform C
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165343.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCAGTGG AGACCATCAA GATGGTGGGT CTGCGCCGAG ATCCCAGCAA GCCGCTGGGC 
CTGACCGTCG AACTGGACGA ATTCAAGCAG CTGGTCGTGG CCAGGATTCT GGCGGGCGGG 
GTGATCGACA AACAGAGCAT GCTGCACGTT GGCGATGTCA TCCTAGAGGT GAACGGTACG 
CCCGTTCGCA CTCCCGATGA GCTGCAGGTG GAGGTGTCGC GGGCCAAGGA GAATCTCACC 
CTCAAGATCG GGCCGAACGT GGACGAGGAG ATCAAGAGCG GTCGCTATAC TGTGAGTGGG 
GGTCAGGTAA AACAGAATGG CATCGCGAGT CTCGAGACGG GCAAGAAACT GACGTGCTAC 
ATGCGTGCCC TTTTCACATA CAATCCATCC GAAGATTCCT TGCTGCCATG CAGGGATATT 
GGATTGCCCT TCAAGTCGGG CGACATTTTG CAGATCATCA ACGTAAAGGA TCCCAACTGG 
TGGCAGGCCA AGAACATTAC TGCCGAATCT GACAAAATTG GACTCATACC ATCCCAAGAG 
CTGGAGGAGC GGCGCAAAGC CTTCGTGGCC CCCGAGGCGG ACTACGTTCA CAAGATTGGC 
ATTTGCGGAA CAAGAATCTC GAAGCGAAAG CGCAAGACCA TGTACCGATC TGTGGCCAAT 
TGCGAGTTCG ACAAGGCGGA GCTGCTGCTC TATGAGGAGG TCACGCGGAT GCCGCCGTTC 
CGCAGGAAAA CCCTGGTTCT CATCGGTGTT TCCGGGGTGG GAAGACGTAC GCTCAAGAAT 
CGGCTGATCA ACAGCGATGT GGACAAGTTC GGAGCGGTCA TTCCACATAC CAGTCGCCCC 
AAGCGCGCCT TGGAGGAGAA CGGCAGTAGC TACTGGTTCA TGGACCGCGA GGAAATGGAA 
GAGGCCGTGC GGAACAACGA GTTCCTGGAG TACGGCGAGC ACAATGGCAA TCTGTACGGC 
ACGCATTTGC AGTCCATCAA GGATGTGATC AACAGTGGGC GCATGTGCAT CCTGGATTGT 
GCACCGAATG CCCTGAAGAT CCTGCACAAC AGCCAGGAAC TGATGCCTTT TGTCATCTTT 
GTGGCAGCGC CTGGCATGGA GCAGCTCAAG ACCATCTATG CGGATCGCAG GGCCACGGGC 
TCCAACCGGA ATTTATCTTT TGATCGCCAG AGTTCCATAA GATTCAGCTC CAGACGCGCC 
CGTACGCTCG AGTCCCTAGC GTCGTTGTAT GAGGACGACG ACCTTGTCGC CACCGTCGAG 
GAGAGCAGCT TCGTCCAGCG CAAGTATGAG AAATACTTCG ACATGGTCAT CGTGAACGAG 
GACTTCGATG AGACGTTCCG CCAGGTGGTG GAAACGCTGG ATCAGATGAG CCACGAGGAG 
CAGTGGGTGC CTGTCAACTG GATCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_724289.2
CDS326..1735
Translation

Target ORF information:

RefSeq Version NM_165343.3
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster varicose (vari), transcript variant C, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_165343.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCAGTGG AGACCATCAA GATGGTGGGT CTGCGCCGAG ATCCCAGCAA GCCGCTGGGC 
CTGACCGTCG AACTGGACGA ATTCAAGCAG CTGGTCGTGG CCAGGATTCT GGCGGGCGGG 
GTGATCGACA AACAGAGCAT GCTGCACGTT GGCGATGTCA TCCTAGAGGT GAACGGTACG 
CCCGTTCGCA CTCCCGATGA GCTGCAGGTG GAGGTGTCGC GGGCCAAGGA GAATCTCACC 
CTCAAGATCG GGCCGAACGT GGACGAGGAG ATCAAGAGCG GTCGCTATAC TGTGAGTGGG 
GGTCAGGTAA AACAGAATGG CATCGCGAGT CTCGAGACGG GCAAGAAACT GACGTGCTAC 
ATGCGTGCCC TTTTCACATA CAATCCATCC GAAGATTCCT TGCTGCCATG CAGGGATATT 
GGATTGCCCT TCAAGTCGGG CGACATTTTG CAGATCATCA ACGTAAAGGA TCCCAACTGG 
TGGCAGGCCA AGAACATTAC TGCCGAATCT GACAAAATTG GACTCATACC ATCCCAAGAG 
CTGGAGGAGC GGCGCAAAGC CTTCGTGGCC CCCGAGGCGG ACTACGTTCA CAAGATTGGC 
ATTTGCGGAA CAAGAATCTC GAAGCGAAAG CGCAAGACCA TGTACCGATC TGTGGCCAAT 
TGCGAGTTCG ACAAGGCGGA GCTGCTGCTC TATGAGGAGG TCACGCGGAT GCCGCCGTTC 
CGCAGGAAAA CCCTGGTTCT CATCGGTGTT TCCGGGGTGG GAAGACGTAC GCTCAAGAAT 
CGGCTGATCA ACAGCGATGT GGACAAGTTC GGAGCGGTCA TTCCACATAC CAGTCGCCCC 
AAGCGCGCCT TGGAGGAGAA CGGCAGTAGC TACTGGTTCA TGGACCGCGA GGAAATGGAA 
GAGGCCGTGC GGAACAACGA GTTCCTGGAG TACGGCGAGC ACAATGGCAA TCTGTACGGC 
ACGCATTTGC AGTCCATCAA GGATGTGATC AACAGTGGGC GCATGTGCAT CCTGGATTGT 
GCACCGAATG CCCTGAAGAT CCTGCACAAC AGCCAGGAAC TGATGCCTTT TGTCATCTTT 
GTGGCAGCGC CTGGCATGGA GCAGCTCAAG ACCATCTATG CGGATCGCAG GGCCACGGGC 
TCCAACCGGA ATTTATCTTT TGATCGCCAG AGTTCCATAA GATTCAGCTC CAGACGCGCC 
CGTACGCTCG AGTCCCTAGC GTCGTTGTAT GAGGACGACG ACCTTGTCGC CACCGTCGAG 
GAGAGCAGCT TCGTCCAGCG CAAGTATGAG AAATACTTCG ACATGGTCAT CGTGAACGAG 
GACTTCGATG AGACGTTCCG CCAGGTGGTG GAAACGCTGG ATCAGATGAG CCACGAGGAG 
CAGTGGGTGC CTGTCAACTG GATCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa15063
Clone ID Related Accession (Same CDS sequence) NM_165343.3 , NM_001273716.1
Accession Version NM_001273716.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product varicose, isoform F
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCAGTGG AGACCATCAA GATGGTGGGT CTGCGCCGAG ATCCCAGCAA GCCGCTGGGC 
CTGACCGTCG AACTGGACGA ATTCAAGCAG CTGGTCGTGG CCAGGATTCT GGCGGGCGGG 
GTGATCGACA AACAGAGCAT GCTGCACGTT GGCGATGTCA TCCTAGAGGT GAACGGTACG 
CCCGTTCGCA CTCCCGATGA GCTGCAGGTG GAGGTGTCGC GGGCCAAGGA GAATCTCACC 
CTCAAGATCG GGCCGAACGT GGACGAGGAG ATCAAGAGCG GTCGCTATAC TGTGAGTGGG 
GGTCAGGTAA AACAGAATGG CATCGCGAGT CTCGAGACGG GCAAGAAACT GACGTGCTAC 
ATGCGTGCCC TTTTCACATA CAATCCATCC GAAGATTCCT TGCTGCCATG CAGGGATATT 
GGATTGCCCT TCAAGTCGGG CGACATTTTG CAGATCATCA ACGTAAAGGA TCCCAACTGG 
TGGCAGGCCA AGAACATTAC TGCCGAATCT GACAAAATTG GACTCATACC ATCCCAAGAG 
CTGGAGGAGC GGCGCAAAGC CTTCGTGGCC CCCGAGGCGG ACTACGTTCA CAAGATTGGC 
ATTTGCGGAA CAAGAATCTC GAAGCGAAAG CGCAAGACCA TGTACCGATC TGTGGCCAAT 
TGCGAGTTCG ACAAGGCGGA GCTGCTGCTC TATGAGGAGG TCACGCGGAT GCCGCCGTTC 
CGCAGGAAAA CCCTGGTTCT CATCGGTGTT TCCGGGGTGG GAAGACGTAC GCTCAAGAAT 
CGGCTGATCA ACAGCGATGT GGACAAGTTC GGAGCGGTCA TTCCACATAC CAGTCGCCCC 
AAGCGCGCCT TGGAGGAGAA CGGCAGTAGC TACTGGTTCA TGGACCGCGA GGAAATGGAA 
GAGGCCGTGC GGAACAACGA GTTCCTGGAG TACGGCGAGC ACAATGGCAA TCTGTACGGC 
ACGCATTTGC AGTCCATCAA GGATGTGATC AACAGTGGGC GCATGTGCAT CCTGGATTGT 
GCACCGAATG CCCTGAAGAT CCTGCACAAC AGCCAGGAAC TGATGCCTTT TGTCATCTTT 
GTGGCAGCGC CTGGCATGGA GCAGCTCAAG ACCATCTATG CGGATCGCAG GGCCACGGGC 
TCCAACCGGA ATTTATCTTT TGATCGCCAG AGTTCCATAA GATTCAGCTC CAGACGCGCC 
CGTACGCTCG AGTCCCTAGC GTCGTTGTAT GAGGACGACG ACCTTGTCGC CACCGTCGAG 
GAGAGCAGCT TCGTCCAGCG CAAGTATGAG AAATACTTCG ACATGGTCAT CGTGAACGAG 
GACTTCGATG AGACGTTCCG CCAGGTGGTG GAAACGCTGG ATCAGATGAG CCACGAGGAG 
CAGTGGGTGC CTGTCAACTG GATCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001260645.1
CDS385..1794
Translation

Target ORF information:

RefSeq Version NM_001273716.1
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster varicose (vari), transcript variant F, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001273716.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCAGTGG AGACCATCAA GATGGTGGGT CTGCGCCGAG ATCCCAGCAA GCCGCTGGGC 
CTGACCGTCG AACTGGACGA ATTCAAGCAG CTGGTCGTGG CCAGGATTCT GGCGGGCGGG 
GTGATCGACA AACAGAGCAT GCTGCACGTT GGCGATGTCA TCCTAGAGGT GAACGGTACG 
CCCGTTCGCA CTCCCGATGA GCTGCAGGTG GAGGTGTCGC GGGCCAAGGA GAATCTCACC 
CTCAAGATCG GGCCGAACGT GGACGAGGAG ATCAAGAGCG GTCGCTATAC TGTGAGTGGG 
GGTCAGGTAA AACAGAATGG CATCGCGAGT CTCGAGACGG GCAAGAAACT GACGTGCTAC 
ATGCGTGCCC TTTTCACATA CAATCCATCC GAAGATTCCT TGCTGCCATG CAGGGATATT 
GGATTGCCCT TCAAGTCGGG CGACATTTTG CAGATCATCA ACGTAAAGGA TCCCAACTGG 
TGGCAGGCCA AGAACATTAC TGCCGAATCT GACAAAATTG GACTCATACC ATCCCAAGAG 
CTGGAGGAGC GGCGCAAAGC CTTCGTGGCC CCCGAGGCGG ACTACGTTCA CAAGATTGGC 
ATTTGCGGAA CAAGAATCTC GAAGCGAAAG CGCAAGACCA TGTACCGATC TGTGGCCAAT 
TGCGAGTTCG ACAAGGCGGA GCTGCTGCTC TATGAGGAGG TCACGCGGAT GCCGCCGTTC 
CGCAGGAAAA CCCTGGTTCT CATCGGTGTT TCCGGGGTGG GAAGACGTAC GCTCAAGAAT 
CGGCTGATCA ACAGCGATGT GGACAAGTTC GGAGCGGTCA TTCCACATAC CAGTCGCCCC 
AAGCGCGCCT TGGAGGAGAA CGGCAGTAGC TACTGGTTCA TGGACCGCGA GGAAATGGAA 
GAGGCCGTGC GGAACAACGA GTTCCTGGAG TACGGCGAGC ACAATGGCAA TCTGTACGGC 
ACGCATTTGC AGTCCATCAA GGATGTGATC AACAGTGGGC GCATGTGCAT CCTGGATTGT 
GCACCGAATG CCCTGAAGAT CCTGCACAAC AGCCAGGAAC TGATGCCTTT TGTCATCTTT 
GTGGCAGCGC CTGGCATGGA GCAGCTCAAG ACCATCTATG CGGATCGCAG GGCCACGGGC 
TCCAACCGGA ATTTATCTTT TGATCGCCAG AGTTCCATAA GATTCAGCTC CAGACGCGCC 
CGTACGCTCG AGTCCCTAGC GTCGTTGTAT GAGGACGACG ACCTTGTCGC CACCGTCGAG 
GAGAGCAGCT TCGTCCAGCG CAAGTATGAG AAATACTTCG ACATGGTCAT CGTGAACGAG 
GACTTCGATG AGACGTTCCG CCAGGTGGTG GAAACGCTGG ATCAGATGAG CCACGAGGAG 
CAGTGGGTGC CTGTCAACTG GATCTACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa15064
Clone ID Related Accession (Same CDS sequence) NM_165342.4
Accession Version NM_165342.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1911bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product varicose, isoform B
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165342.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTGTGAGTGG GGGTCAGGTA AAACAGAATG GCATCGCGAG TCTCGAGACG 
GGCAAGAAAC TGACGTGCTA CATGCGTGCC CTTTTCACAT ACAATCCATC CGAAGATTCC 
TTGCTGCCAT GCAGGGATAT TGGATTGCCC TTCAAGTCGG GCGACATTTT GCAGATCATC 
AACGTAAAGG ATCCCAACTG GTGGCAGGCC AAGAACATTA CTGCCGAATC TGACAAAATT 
GGACTCATAC CATCCCAAGA GCTGGAGGAG CGGCGCAAAG CCTTCGTGGC CCCCGAGGCG 
GACTACGTTC ACAAGATTGG CATTTGCGGA ACAAGAATCT CGAAGCGAAA GCGCAAGACC 
ATGTACCGAT CTGTGGCCAA TTGCGAGTTC GACAAGGCGG AGCTGCTGCT CTATGAGGAG 
GTCACGCGGA TGCCGCCGTT CCGCAGGAAA ACCCTGGTTC TCATCGGTGT TTCCGGGGTG 
GGAAGACGTA CGCTCAAGAA TCGGCTGATC AACAGCGATG TGGACAAGTT CGGAGCGGTC 
ATTCCACATA CCAGTCGCCC CAAGCGCGCC TTGGAGGAGA ACGGCAGTAG CTACTGGTTC 
ATGGACCGCG AGGAAATGGA AGAGGCCGTG CGGAACAACG AGTTCCTGGA GTACGGCGAG 
CACAATGGCA ATCTGTACGG CACGCATTTG CAGTCCATCA AGGATGTGAT CAACAGTGGG 
CGCATGTGCA TCCTGGATTG TGCACCGAAT GCCCTGAAGA TCCTGCACAA CAGCCAGGAA 
CTGATGCCTT TTGTCATCTT TGTGGCAGCG CCTGGCATGG AGCAGCTCAA GACCATCTAT 
GCGGATCGCA GGGCCACGGG CTCCAACCGG AATTTATCTT TTGATCGCCA GAGTTCCATA 
AGATTCAGCT CCAGACGCGC CCGTACGCTC GAGTCCCTAG CGTCGTTGTA TGAGGACGAC 
GACCTTGTCG CCACCGTCGA GGAGAGCAGC TTCGTCCAGC GCAAGTATGA GAAATACTTC 
GACATGGTCA TCGTGAACGA GGACTTCGAT GAGACGTTCC GCCAGGTGGT GGAAACGCTG 
GATCAGATGA GCCACGAGGA GCAGTGGGTG CCTGTCAACT GGATCTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_724288.3
CDS285..2195
Translation

Target ORF information:

RefSeq Version NM_165342.4
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster varicose (vari), transcript variant B, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_165342.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTGTGAGTGG GGGTCAGGTA AAACAGAATG GCATCGCGAG TCTCGAGACG 
GGCAAGAAAC TGACGTGCTA CATGCGTGCC CTTTTCACAT ACAATCCATC CGAAGATTCC 
TTGCTGCCAT GCAGGGATAT TGGATTGCCC TTCAAGTCGG GCGACATTTT GCAGATCATC 
AACGTAAAGG ATCCCAACTG GTGGCAGGCC AAGAACATTA CTGCCGAATC TGACAAAATT 
GGACTCATAC CATCCCAAGA GCTGGAGGAG CGGCGCAAAG CCTTCGTGGC CCCCGAGGCG 
GACTACGTTC ACAAGATTGG CATTTGCGGA ACAAGAATCT CGAAGCGAAA GCGCAAGACC 
ATGTACCGAT CTGTGGCCAA TTGCGAGTTC GACAAGGCGG AGCTGCTGCT CTATGAGGAG 
GTCACGCGGA TGCCGCCGTT CCGCAGGAAA ACCCTGGTTC TCATCGGTGT TTCCGGGGTG 
GGAAGACGTA CGCTCAAGAA TCGGCTGATC AACAGCGATG TGGACAAGTT CGGAGCGGTC 
ATTCCACATA CCAGTCGCCC CAAGCGCGCC TTGGAGGAGA ACGGCAGTAG CTACTGGTTC 
ATGGACCGCG AGGAAATGGA AGAGGCCGTG CGGAACAACG AGTTCCTGGA GTACGGCGAG 
CACAATGGCA ATCTGTACGG CACGCATTTG CAGTCCATCA AGGATGTGAT CAACAGTGGG 
CGCATGTGCA TCCTGGATTG TGCACCGAAT GCCCTGAAGA TCCTGCACAA CAGCCAGGAA 
CTGATGCCTT TTGTCATCTT TGTGGCAGCG CCTGGCATGG AGCAGCTCAA GACCATCTAT 
GCGGATCGCA GGGCCACGGG CTCCAACCGG AATTTATCTT TTGATCGCCA GAGTTCCATA 
AGATTCAGCT CCAGACGCGC CCGTACGCTC GAGTCCCTAG CGTCGTTGTA TGAGGACGAC 
GACCTTGTCG CCACCGTCGA GGAGAGCAGC TTCGTCCAGC GCAAGTATGA GAAATACTTC 
GACATGGTCA TCGTGAACGA GGACTTCGAT GAGACGTTCC GCCAGGTGGT GGAAACGCTG 
GATCAGATGA GCCACGAGGA GCAGTGGGTG CCTGTCAACT GGATCTACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OFa16954
Clone ID Related Accession (Same CDS sequence) NM_001273715.1
Accession Version NM_001273715.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1836bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-24
Organism Drosophila melanogaster(fruit fly)
Product varicose, isoform E
Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTGTGAGTGG GGGTCAGGTA AAACAGAATG GCATCGCGAG TCTCGAGACG 
GGCAAGAAAC TGACGTGCTA CATGCGTGCC CTTTTCACAT ACAATCCATC CGAAGATTCC 
TTGCTGCCAT GCAGGGATAT TGGATTGCCC TTCAAGTCGG GCGACATTTT GCAGATCATC 
AACGTAAAGG ATCCCAACTG GTGGCAGGCC AAGAACATTA CTGCCGAATC TGACAAAATT 
GGACTCATAC CATCCCAAGA GCTGGAGGAG CGGCGCAAAG CCTTCGTGGC CCCCGAGGCG 
GACTACGTTC ACAAGATTGG CATTTGCGGA ACAAGAATCT CGAAGCGAAA GCGCAAGACC 
ATGTACCGAT CTGTGGCCAA TTGCGAGTTC GACAAGGCGG AGCTGCTGCT CTATGAGGAG 
GTCACGCGGA TGCCGCCGTT CCGCAGGAAA ACCCTGGTTC TCATCGGTGT TTCCGGGGTG 
GGAAGACGTA CGCTCAAGAA TCGGCTGATC AACAGCGATG TGGACAAGTT CGGAGCGGTC 
ATTCCACATA CCAGTCGCCC CAAGCGCGCC TTGGAGGAGA ACGGCAGTAG CTACTGGTTC 
ATGGACCGCG AGGAAATGGA AGAGGCCGTG CGGAACAACG AGTTCCTGGA GTACGGCGAG 
CACAATGGCA ATCTGTACGG CACGCATTTG CAGTCCATCA AGGATGTGAT CAACAGTGGG 
CGCATGTGCA TCCTGGATTG TGCACCGAAT GCCCTGAAGA TCCTGCACAA CAGCCAGGAA 
CTGATGCCTT TTGTCATCTT TGTGGCAGCG CCTGGCATGG AGCAGCTCAA GACCATCTAT 
GCGGATCGCA GGGCCACGGG CTCCAACCGG AATTTATCTG ACGACGACCT TGTCGCCACC 
GTCGAGGAGA GCAGCTTCGT CCAGCGCAAG TATGAGAAAT ACTTCGACAT GGTCATCGTG 
AACGAGGACT TCGATGAGAC GTTCCGCCAG GTGGTGGAAA CGCTGGATCA GATGAGCCAC 
GAGGAGCAGT GGGTGCCTGT CAACTGGATC TACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001260644.1
CDS285..2120
Translation

Target ORF information:

RefSeq Version NM_001273715.1
Organism Drosophila melanogaster(fruit fly)
Definition Drosophila melanogaster varicose (vari), transcript variant E, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001273715.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGTTCGGT GGAGCGCGCG GTCGCGGCGC CAAGCTCGCC AGGATAAAGT GGAGCTATTG 
GCCAGAAACA ACAAAGTGAA TGGCGAGGAC GATACCTCCG ATAACGCCGC CTTTCGCAAC 
TCGACTGACC TATCCGACCA CGATGAGATC TTCCTCAAGG GATTGCTGCG TAGTAACTCC 
AACACGCCCC ACAAGGAACT GATGCTGAAT CCGACCGAGC CGCAGCCAGT GCCACTATTC 
CTGCCCGCTC ATCTGAACAA CAAGCCCATC TGCGACGACA TCATCCGCAA GTTCTCACCC 
TCGAGGCGCC TGGAGTCGCG AGAGCTCGCC AAGCTTCTCG CCCAGCCGCA TTTTCGTGCT 
CTTTTGCGCG CCCATGATGA GATAGGAGCA CTCTACGAGC AACGACTTAA AGCTGCCGGG 
GGATCCACCA GCCAACTAGA GATCGCCAGC CAACGCCAAA CGGGAGGCTA CCTGTTCACC 
GAGGACGTCC TCAACACCAA GATGCCAGTG GAGACCATCA AGATGGTGGG TCTGCGCCGA 
GATCCCAGCA AGCCGCTGGG CCTGACCGTC GAACTGGACG AATTCAAGCA GCTGGTCGTG 
GCCAGGATTC TGGCGGGCGG GGTGATCGAC AAACAGAGCA TGCTGCACGT TGGCGATGTC 
ATCCTAGAGG TGAACGGTAC GCCCGTTCGC ACTCCCGATG AGCTGCAGGT GGAGGTGTCG 
CGGGCCAAGG AGAATCTCAC CCTCAAGATC GGGCCGAACG TGGACGAGGA GATCAAGAGC 
GGTCGCTATA CTGTGAGTGG GGGTCAGGTA AAACAGAATG GCATCGCGAG TCTCGAGACG 
GGCAAGAAAC TGACGTGCTA CATGCGTGCC CTTTTCACAT ACAATCCATC CGAAGATTCC 
TTGCTGCCAT GCAGGGATAT TGGATTGCCC TTCAAGTCGG GCGACATTTT GCAGATCATC 
AACGTAAAGG ATCCCAACTG GTGGCAGGCC AAGAACATTA CTGCCGAATC TGACAAAATT 
GGACTCATAC CATCCCAAGA GCTGGAGGAG CGGCGCAAAG CCTTCGTGGC CCCCGAGGCG 
GACTACGTTC ACAAGATTGG CATTTGCGGA ACAAGAATCT CGAAGCGAAA GCGCAAGACC 
ATGTACCGAT CTGTGGCCAA TTGCGAGTTC GACAAGGCGG AGCTGCTGCT CTATGAGGAG 
GTCACGCGGA TGCCGCCGTT CCGCAGGAAA ACCCTGGTTC TCATCGGTGT TTCCGGGGTG 
GGAAGACGTA CGCTCAAGAA TCGGCTGATC AACAGCGATG TGGACAAGTT CGGAGCGGTC 
ATTCCACATA CCAGTCGCCC CAAGCGCGCC TTGGAGGAGA ACGGCAGTAG CTACTGGTTC 
ATGGACCGCG AGGAAATGGA AGAGGCCGTG CGGAACAACG AGTTCCTGGA GTACGGCGAG 
CACAATGGCA ATCTGTACGG CACGCATTTG CAGTCCATCA AGGATGTGAT CAACAGTGGG 
CGCATGTGCA TCCTGGATTG TGCACCGAAT GCCCTGAAGA TCCTGCACAA CAGCCAGGAA 
CTGATGCCTT TTGTCATCTT TGTGGCAGCG CCTGGCATGG AGCAGCTCAA GACCATCTAT 
GCGGATCGCA GGGCCACGGG CTCCAACCGG AATTTATCTG ACGACGACCT TGTCGCCACC 
GTCGAGGAGA GCAGCTTCGT CCAGCGCAAG TATGAGAAAT ACTTCGACAT GGTCATCGTG 
AACGAGGACT TCGATGAGAC GTTCCGCCAG GTGGTGGAAA CGCTGGATCA GATGAGCCAC 
GAGGAGCAGT GGGTGCCTGT CAACTGGATC TACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,


book

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,


book

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE


book

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH


book

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D