Spn53F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spn53F
Entrez Gene ID	36931
Full Name	Serpin 53F
Synonyms	CG10956,Dmel\CG10956
General protein information	Preferred Names Serpin 53F Names CG10956 gene product from transcript CG10956-RB CG10956-PB Spn53F-PB serpin 53F
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53F12-53F12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043088.2	NP_001036553.1	serpin 53F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spn53F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spn53F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10327	NM_001043088.2 Latest version!	Drosophila melanogaster serpin 53F (Spn53F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spn53F ( NM_001043088.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036553.1 Drosophila melanogaster serpin 53F (Spn53F), mRNA.

Price & Availability↑

CloneID	OFa10327
Clone ID Related Accession (Same CDS sequence)	NM_001043088.2
Accession Version	NM_001043088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serpin 53F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001043088.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACGTGC TACTGCTAAT CCTTTTAGGA ATCTCACGCT ACAGGGCTCA GAAAAATTCA 
CAATTGCCAG ATGAACTTTA CGCAGCCATC GTGAACAGCT TTTCGAACAG GAACATTATG 
TTCTCGACGG AGATGATACG ATCCAGCATG CTGTTCATCT ATGTGGGCGT GGAGGAAGAT 
GAATCCGAGC AAATTCGAAA GGCTATGCAC TATCGAGGAA CCCATCTATC TGAATACAAG 
CCGAAAACAC AGAAAATCTT TGCCATGAGT GTGAAGAAGG CACCTGTGGC CAAGAGTCTT 
ACCCGATTCT ATGTACGTCA GAATATGAAA ATGTCCACGG AATATAGAGT TTTTATGCGG 
CATACAGAGG GAAGAGCTCG TAATATCGCC TTTGCTCGTG AACAGCTAGA CGAGGTTAAT 
ACATTTTACA GCCATGAAAT GGGCGAGCAA ATTGGGCAGG TGGTAAAAGA AAGCTGGTGG 
AAACCGAACA GCCAAGGACT TCTCGTAAAT GCCATTTTCT TCAATTTGAG TTGGGAGCGG 
ACATTCAATC CGGAGGCCAC ATATCCCCGG GAGTTTCGAG TAAATGCCAC AAAGAGCGTT 
ATGATACCCA TGATGCACGA GGATAGTAAG TTTGCATTTG GCATCCTAGG GAATCTTAAA 
GCCACCGCCG TCCTGGTGCC TTTTTCCCAT GGAGATCTCC GAATGCTCTT GATAAAGCCC 
GATCAGCCTG ATGGACTTGC TGCCTTGCAA ATGAAGCTGC AGGCCATGAA TATCTTAAGC 
GTTGCCAGGA ACCTCACTAT GATGGATGTG TTTGTGGGTA TACCGAAATT TAAGATACAC 
AGTGATCTAG AACTCTCTCC AGCTTTTGAA AAGATGGGCA TCAAGGATAT ATTTAAGCCC 
AGCAAGTCAT TTTCAACGTT GCTTCACAGG AATACCAACT TTCGGATTGA CGGAGTTATT 
CATGTAGTCA CCTTTGAGTT TCAGGAGCAG GGCATTGGTA CGCCAAGTAC AGATGTTGGC 
AATGGTAGTT TGACACACAC CTTTAATGGG GTGAAATATT TTTTGGCTAC TCATCCATTT 
GCTTTTTATA TCATAGACAA CACATCAATA TATTTTGCCG GTCATGTAAC AAGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036553.1
CDS	11..1150
Translation	MYVLLLILLGISRYRAQKNSQLPDELYAAIVNSFSNRNIMFSTEMIRSSMLFIYVGVEEDESEQIRKAMHYRGTHLSEYKPKTQKIFAMSVKKAPVAKSLTRFYVRQNMKMSTEYRVFMRHTEGRARNIAFAREQLDEVNTFYSHEMGEQIGQVVKESWWKPNSQGLLVNAIFFNLSWERTFNPEATYPREFRVNATKSVMIPMMHEDSKFAFGILGNLKATAVLVPFSHGDLRMLLIKPDQPDGLAALQMKLQAMNILSVARNLTMMDVFVGIPKFKIHSDLELSPAFEKMGIKDIFKPSKSFSTLLHRNTNFRIDGVIHVVTFEFQEQGIGTPSTDVGNGSLTHTFNGVKYFLATHPFAFYIIDNTSIYFAGHVTSF

Target ORF information:

RefSeq Version	NM_001043088.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serpin 53F (Spn53F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043088.2

ORF Insert Sequence:

ATGTACGTGC TACTGCTAAT CCTTTTAGGA ATCTCACGCT ACAGGGCTCA GAAAAATTCA 
CAATTGCCAG ATGAACTTTA CGCAGCCATC GTGAACAGCT TTTCGAACAG GAACATTATG 
TTCTCGACGG AGATGATACG ATCCAGCATG CTGTTCATCT ATGTGGGCGT GGAGGAAGAT 
GAATCCGAGC AAATTCGAAA GGCTATGCAC TATCGAGGAA CCCATCTATC TGAATACAAG 
CCGAAAACAC AGAAAATCTT TGCCATGAGT GTGAAGAAGG CACCTGTGGC CAAGAGTCTT 
ACCCGATTCT ATGTACGTCA GAATATGAAA ATGTCCACGG AATATAGAGT TTTTATGCGG 
CATACAGAGG GAAGAGCTCG TAATATCGCC TTTGCTCGTG AACAGCTAGA CGAGGTTAAT 
ACATTTTACA GCCATGAAAT GGGCGAGCAA ATTGGGCAGG TGGTAAAAGA AAGCTGGTGG 
AAACCGAACA GCCAAGGACT TCTCGTAAAT GCCATTTTCT TCAATTTGAG TTGGGAGCGG 
ACATTCAATC CGGAGGCCAC ATATCCCCGG GAGTTTCGAG TAAATGCCAC AAAGAGCGTT 
ATGATACCCA TGATGCACGA GGATAGTAAG TTTGCATTTG GCATCCTAGG GAATCTTAAA 
GCCACCGCCG TCCTGGTGCC TTTTTCCCAT GGAGATCTCC GAATGCTCTT GATAAAGCCC 
GATCAGCCTG ATGGACTTGC TGCCTTGCAA ATGAAGCTGC AGGCCATGAA TATCTTAAGC 
GTTGCCAGGA ACCTCACTAT GATGGATGTG TTTGTGGGTA TACCGAAATT TAAGATACAC 
AGTGATCTAG AACTCTCTCC AGCTTTTGAA AAGATGGGCA TCAAGGATAT ATTTAAGCCC 
AGCAAGTCAT TTTCAACGTT GCTTCACAGG AATACCAACT TTCGGATTGA CGGAGTTATT 
CATGTAGTCA CCTTTGAGTT TCAGGAGCAG GGCATTGGTA CGCCAAGTAC AGATGTTGGC 
AATGGTAGTT TGACACACAC CTTTAATGGG GTGAAATATT TTTTGGCTAC TCATCCATTT 
GCTTTTTATA TCATAGACAA CACATCAATA TATTTTGCCG GTCATGTAAC AAGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Spn53F cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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