Sec8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Gene Symbol	Sec8
Entrez Gene ID	40712
Full Name	Secretory 8
Synonyms	CG2095,Dmel\CG2095,Sec8p,dsec8,fun,sec8
General protein information	Preferred Names Secretory 8 Names CG2095 gene product from transcript CG2095-RA CG2095-PA Sec8 ortholog Sec8-PA funnel cakes sec8 secretory 8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83C2-83C2\|3-48.8 cM

mRNA and Protein(s)

mRNA Protein Name

NM_169098.4 NP_730996.2 secretory 8
Pathways from BioSystems

mRNA	Protein	Name
NM_169098.4	NP_730996.2	secretory 8

Homologies

	EXOC4	XP_001101640.2
Canis lupus familiaris (dog)	EXOC4	XP_539369.2
Mus musculus (house mouse)	Exoc4	NP_033174.2
Rattus norvegicus (Norway rat)	Exoc4	NP_446327.1
Pan troglodytes (chimpanzee)	EXOC4	XP_001139207.1
Drosophila melanogaster (fruit fly)	sec8	NP_730996.2
Xenopus tropicalis (tropical clawed frog)	exoc4	XP_002931915.2
Homo sapiens (human)	EXOC4	NP_068579.3
Gallus gallus (chicken)	EXOC4	NP_001186249.1
Bos taurus (cattle)	EXOC4	NP_001095677.1
Danio rerio (zebrafish)	exoc4	NP_001025305.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001694	XP_321399.5
Caenorhabditis elegans (roundworm)	sec-8	NP_492732.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

No evidence that Sec8 is required for basal neurotransmission, though glutamate receptor trafficking was mildly disrupted in sec8 mutants.
Title: Increased synaptic microtubules and altered synapse development in Drosophila sec8 mutants.

Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function
Title: Sec6 mutations and the Drosophila exocyst complex.

The following Sec8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sec8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03994	NM_169098.4 Latest version!	Drosophila melanogaster secretory 8 (Sec8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sec8 ( NM_169098.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730996.2 Drosophila melanogaster secretory 8 (Sec8), mRNA.

Price & Availability↑

CloneID	OFa03994
Clone ID Related Accession (Same CDS sequence)	NM_169098.4
Accession Version	NM_169098.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169098.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCC CACCGCCCAC GAAGCCCCCT AGGGGTGTGA AGTACGGCAA GGATGAGTCC 
GCAGGATGTG GCTTCCTGGT CAACGTCATC AAGTCCCTGG GATTCAGCGA GACCACCGAG 
GAGCGCCAGA AGGAGAAGCA GAAGATCGAG GCGGAATTCA AGCGCTCGGA TCTGCGCTTG 
AACGAGCTGG TCTCGCGGCA CGACCAGCAG CTTACCCAGG TGCTGCCCCT GTTCAGCCAG 
GTGTCCTCGG AGGTGACGGC CAGCCGGGAG CGGATTCATG CAGTCAAGGA GAACCTTGGT 
GTCTGCAAGC GGCTGCTGCA GTGCCGGCGC GATGAGCTCC GGAAGATGTG GATGGATGCG 
GTGCAGCACA AGTACGTTCT GGAGATGCTG GAGCAAATCC AGGAGCTGCG CAAAGTGCCT 
CAAAGGGTGG TGGGCTACAC CGCCAAACGA CAGTATCTGC ACGCCAGCAA GGCCCTAACT 
GATGCCCTGA CCACCCTCAA TGGACCCTTA CAGGCCGTCG AGGGCTTGTC AGATTTGCGA 
ACTGACCTGC AAACCCGACG CCAGCAGCTG TATCAGCGGC TGCACGAGGA ACTGGTCACC 
CAGGTGTACA CCAACTCGGC CAACGAGGCC CTCTCTTCGT TCCAACGCAC CAACTCCAGT 
AGGCTGAATT CCAGTTTTAC TCGGGGAATA GGTGCTCGTC GCTCTACGGA TCGCATCGAG 
GCCAATGCTC GAGTGCGCAA AGCGCTGGCC GAAATGGCGC AGAGCTTCGA TTTGGACAAG 
GCAGAGGTTA TCGAAGACGC AGATCTCATT TACCCGGAGC TGAGCATGAG CTACTTCGTT 
GCCATCATAG TGGAGTCGTT CGGAATGCTG CACAAGGTGC CTGACTCTCT GGAGACACTG 
CGCGTCCAAA TCCAAACAGA GTTGCTCAAC GTGGTGCGGC ACACAACGCA TCAACTGTCT 
GTGAGTGGAG CAACGGCGGA CACCAATCCG CTCCTTTCAC TTCTGGAGGT CATATTCAAG 
CAGTTCAAGG CAATCGCCAA AACGCATTCC TTGTTGCTGA AGAACTATTT GTCGGTGGGA 
CAGAAGTACT CTGTTGTTGG ACCGCAACCA TACGATCTAA CCGATTTCTG GGCCCAGGCT 
CAGTCAGTGT TGCAACTGTT GCTCACCGAC TACTTGGATA TACAGAATGC TGCCGCGGAC 
GAAAGTGCCC AGACGGGGTT TTCAGAGCCC ACCAGCAACA TAAATTCCTA TTTTCTGAGG 
CGCAAGGTGC CGAGCACCAA GCGATCAATG TTCAAGTTCG ATAAGTCCTC GCACGTTGGC 
ACGAGCAACA ATAGCGACGC CTTCAAGGAG CATCGTCGCA ACGCCTCGGA TGCCTCCGTG 
GATGACAACC TTGCCGGCCA GCTTGGTGGC AGTGGCAAGG GCTCCACCAG TGGCCTCTTT 
CCTCACGAGA AAAAGCAGCG GGAAAAAATA CTTATTTGTA CGCCCGATCA AAGTATCATA 
ACCAAAGTGT ATCTACCTCT AATGGGATAT ATCAAGGAGA TAGAGAACTT TATGAAATGC 
AAGCCAGGGC AACCCTGCAG TCTACATGAC TTTTTAGACA ACTACATTAA GGACACATTC 
CTAACCAAAG GCCACAACCG CAACCTGCAG CTGACCATAG AGTCGCTGTC GAAGAATCAA 
GACGCATGGC GCACTATTAT AAGTCCTGAG GAAATAAAGG CTCTTAATCT AAGCAGGCCG 
TTGCTGCAGT CTACTGTGAT GGTCGAACGG AGGTTGATGG AAACTAAGAA CCTCATTCAA 
GACTTGCCCT GCTACTCCGA AGACCTGCTT AAAATGGTGT GCGCGTTGTT GAAGGCCTAT 
CGGGAAATAT GTCAGGCGGC CTATCGCGGA ATCGTGCAGC CAGATTCAGA GGATAAGAGA 
ATTTACAGCG TGGCATGGCT TAAAGATGAA GATATCAGCA GGTTTTTAAA AACGCTTCCC 
AACTGGACTG ATTTAAAGAC CTACAGTCAG AAGTCGCGCC ACAACCGAAA ACTGCATCGC 
GGAAGCTTTG AGCCGTCGGA GGAGGAGAGC CCGTTGCAGG TTCAGCAGCG CAACATCCGC 
GAGGCTGAAA TGCTGACCAG TAATTTAGGC GAGGGTGGGA TCACCCAACA AGAGATTTTG 
GTCGAGATCA GTGTGCTTAA GGAGCTGGCC ATACTGCAGG AGAGCATGGA GTGGTTTTCG 
TGCCGAGTGT CGGAATTTGC CAACGACTTA CGCCGGCCGT TGGTAAACGG ACTTAACGCC 
GTTCCAGCTG AGTGCGGCGC CGACATTGCT GTGAAGGATG GGACGATCAA AGTGATGACT 
AATCTGGCTT TGGAGTTTGA TGAGCTGGCG AACACCTGCC TGCTGGTATT GCATCTGGAG 
GTGCGCGTTC AATGCTTCCA CTATCTGCGC TCGAAGTCGA GTGTAAGGAC CAACAGCTAT 
GTGGGCTCCA AGGATGATAT CTTGGAGCCC GACCGCCAGG TGCAAGTTCT GACCAAACGA 
CTGTCTGAGA TGGATGAGGC GTTTAGTGCC ACTCTACATC CGAGGAAGAC CAGGTACATT 
TTCGAGGGTC TGGCGCACTT GGCATCGCGC ATTCTTATCC AGGCCTCCAA TTATCTGGAG 
CACATAGATC AAATAACCGT GCAGCGCATG TGCAGGAACG CGATTGCCCT GCAGCAAACA 
TTGAGTAACA TAACTGCCTC CAGGGAAGTG GCTTTGGATC AGGCGAGACA CTTCTATGAG 
CTGCTTTGCA TGGAGCCAGA CGAAATACTC AATGCGTTGC TGGAGCGGGG CACTCAGTTC 
TCGGAAATGC AGCTGCTCAA TGCCTTGCAG TTGTCCTGCA AGTCCTTCGG CATAACCGAC 
GCCAATCTCC TCGCTTCCTA TCAGCAGAAG CTGTCGGACA TACTAGGCGC CAAGCCCTCG 
AAGGGAGTAG TTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730996.2
CDS	180..3137
Translation	MDAPPPTKPPRGVKYGKDESAGCGFLVNVIKSLGFSETTEERQKEKQKIEAEFKRSDLRLNELVSRHDQQLTQVLPLFSQVSSEVTASRERIHAVKENLGVCKRLLQCRRDELRKMWMDAVQHKYVLEMLEQIQELRKVPQRVVGYTAKRQYLHASKALTDALTTLNGPLQAVEGLSDLRTDLQTRRQQLYQRLHEELVTQVYTNSANEALSSFQRTNSSRLNSSFTRGIGARRSTDRIEANARVRKALAEMAQSFDLDKAEVIEDADLIYPELSMSYFVAIIVESFGMLHKVPDSLETLRVQIQTELLNVVRHTTHQLSVSGATADTNPLLSLLEVIFKQFKAIAKTHSLLLKNYLSVGQKYSVVGPQPYDLTDFWAQAQSVLQLLLTDYLDIQNAAADESAQTGFSEPTSNINSYFLRRKVPSTKRSMFKFDKSSHVGTSNNSDAFKEHRRNASDASVDDNLAGQLGGSGKGSTSGLFPHEKKQREKILICTPDQSIITKVYLPLMGYIKEIENFMKCKPGQPCSLHDFLDNYIKDTFLTKGHNRNLQLTIESLSKNQDAWRTIISPEEIKALNLSRPLLQSTVMVERRLMETKNLIQDLPCYSEDLLKMVCALLKAYREICQAAYRGIVQPDSEDKRIYSVAWLKDEDISRFLKTLPNWTDLKTYSQKSRHNRKLHRGSFEPSEEESPLQVQQRNIREAEMLTSNLGEGGITQQEILVEISVLKELAILQESMEWFSCRVSEFANDLRRPLVNGLNAVPAECGADIAVKDGTIKVMTNLALEFDELANTCLLVLHLEVRVQCFHYLRSKSSVRTNSYVGSKDDILEPDRQVQVLTKRLSEMDEAFSATLHPRKTRYIFEGLAHLASRILIQASNYLEHIDQITVQRMCRNAIALQQTLSNITASREVALDQARHFYELLCMEPDEILNALLERGTQFSEMQLLNALQLSCKSFGITDANLLASYQQKLSDILGAKPSKGVVV

Target ORF information:

RefSeq Version	NM_169098.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 8 (Sec8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169098.4

ORF Insert Sequence:

ATGGACGCCC CACCGCCCAC GAAGCCCCCT AGGGGTGTGA AGTACGGCAA GGATGAGTCC 
GCAGGATGTG GCTTCCTGGT CAACGTCATC AAGTCCCTGG GATTCAGCGA GACCACCGAG 
GAGCGCCAGA AGGAGAAGCA GAAGATCGAG GCGGAATTCA AGCGCTCGGA TCTGCGCTTG 
AACGAGCTGG TCTCGCGGCA CGACCAGCAG CTTACCCAGG TGCTGCCCCT GTTCAGCCAG 
GTGTCCTCGG AGGTGACGGC CAGCCGGGAG CGGATTCATG CAGTCAAGGA GAACCTTGGT 
GTCTGCAAGC GGCTGCTGCA GTGCCGGCGC GATGAGCTCC GGAAGATGTG GATGGATGCG 
GTGCAGCACA AGTACGTTCT GGAGATGCTG GAGCAAATCC AGGAGCTGCG CAAAGTGCCT 
CAAAGGGTGG TGGGCTACAC CGCCAAACGA CAGTATCTGC ACGCCAGCAA GGCCCTAACT 
GATGCCCTGA CCACCCTCAA TGGACCCTTA CAGGCCGTCG AGGGCTTGTC AGATTTGCGA 
ACTGACCTGC AAACCCGACG CCAGCAGCTG TATCAGCGGC TGCACGAGGA ACTGGTCACC 
CAGGTGTACA CCAACTCGGC CAACGAGGCC CTCTCTTCGT TCCAACGCAC CAACTCCAGT 
AGGCTGAATT CCAGTTTTAC TCGGGGAATA GGTGCTCGTC GCTCTACGGA TCGCATCGAG 
GCCAATGCTC GAGTGCGCAA AGCGCTGGCC GAAATGGCGC AGAGCTTCGA TTTGGACAAG 
GCAGAGGTTA TCGAAGACGC AGATCTCATT TACCCGGAGC TGAGCATGAG CTACTTCGTT 
GCCATCATAG TGGAGTCGTT CGGAATGCTG CACAAGGTGC CTGACTCTCT GGAGACACTG 
CGCGTCCAAA TCCAAACAGA GTTGCTCAAC GTGGTGCGGC ACACAACGCA TCAACTGTCT 
GTGAGTGGAG CAACGGCGGA CACCAATCCG CTCCTTTCAC TTCTGGAGGT CATATTCAAG 
CAGTTCAAGG CAATCGCCAA AACGCATTCC TTGTTGCTGA AGAACTATTT GTCGGTGGGA 
CAGAAGTACT CTGTTGTTGG ACCGCAACCA TACGATCTAA CCGATTTCTG GGCCCAGGCT 
CAGTCAGTGT TGCAACTGTT GCTCACCGAC TACTTGGATA TACAGAATGC TGCCGCGGAC 
GAAAGTGCCC AGACGGGGTT TTCAGAGCCC ACCAGCAACA TAAATTCCTA TTTTCTGAGG 
CGCAAGGTGC CGAGCACCAA GCGATCAATG TTCAAGTTCG ATAAGTCCTC GCACGTTGGC 
ACGAGCAACA ATAGCGACGC CTTCAAGGAG CATCGTCGCA ACGCCTCGGA TGCCTCCGTG 
GATGACAACC TTGCCGGCCA GCTTGGTGGC AGTGGCAAGG GCTCCACCAG TGGCCTCTTT 
CCTCACGAGA AAAAGCAGCG GGAAAAAATA CTTATTTGTA CGCCCGATCA AAGTATCATA 
ACCAAAGTGT ATCTACCTCT AATGGGATAT ATCAAGGAGA TAGAGAACTT TATGAAATGC 
AAGCCAGGGC AACCCTGCAG TCTACATGAC TTTTTAGACA ACTACATTAA GGACACATTC 
CTAACCAAAG GCCACAACCG CAACCTGCAG CTGACCATAG AGTCGCTGTC GAAGAATCAA 
GACGCATGGC GCACTATTAT AAGTCCTGAG GAAATAAAGG CTCTTAATCT AAGCAGGCCG 
TTGCTGCAGT CTACTGTGAT GGTCGAACGG AGGTTGATGG AAACTAAGAA CCTCATTCAA 
GACTTGCCCT GCTACTCCGA AGACCTGCTT AAAATGGTGT GCGCGTTGTT GAAGGCCTAT 
CGGGAAATAT GTCAGGCGGC CTATCGCGGA ATCGTGCAGC CAGATTCAGA GGATAAGAGA 
ATTTACAGCG TGGCATGGCT TAAAGATGAA GATATCAGCA GGTTTTTAAA AACGCTTCCC 
AACTGGACTG ATTTAAAGAC CTACAGTCAG AAGTCGCGCC ACAACCGAAA ACTGCATCGC 
GGAAGCTTTG AGCCGTCGGA GGAGGAGAGC CCGTTGCAGG TTCAGCAGCG CAACATCCGC 
GAGGCTGAAA TGCTGACCAG TAATTTAGGC GAGGGTGGGA TCACCCAACA AGAGATTTTG 
GTCGAGATCA GTGTGCTTAA GGAGCTGGCC ATACTGCAGG AGAGCATGGA GTGGTTTTCG 
TGCCGAGTGT CGGAATTTGC CAACGACTTA CGCCGGCCGT TGGTAAACGG ACTTAACGCC 
GTTCCAGCTG AGTGCGGCGC CGACATTGCT GTGAAGGATG GGACGATCAA AGTGATGACT 
AATCTGGCTT TGGAGTTTGA TGAGCTGGCG AACACCTGCC TGCTGGTATT GCATCTGGAG 
GTGCGCGTTC AATGCTTCCA CTATCTGCGC TCGAAGTCGA GTGTAAGGAC CAACAGCTAT 
GTGGGCTCCA AGGATGATAT CTTGGAGCCC GACCGCCAGG TGCAAGTTCT GACCAAACGA 
CTGTCTGAGA TGGATGAGGC GTTTAGTGCC ACTCTACATC CGAGGAAGAC CAGGTACATT 
TTCGAGGGTC TGGCGCACTT GGCATCGCGC ATTCTTATCC AGGCCTCCAA TTATCTGGAG 
CACATAGATC AAATAACCGT GCAGCGCATG TGCAGGAACG CGATTGCCCT GCAGCAAACA 
TTGAGTAACA TAACTGCCTC CAGGGAAGTG GCTTTGGATC AGGCGAGACA CTTCTATGAG 
CTGCTTTGCA TGGAGCCAGA CGAAATACTC AATGCGTTGC TGGAGCGGGG CACTCAGTTC 
TCGGAAATGC AGCTGCTCAA TGCCTTGCAG TTGTCCTGCA AGTCCTTCGG CATAACCGAC 
GCCAATCTCC TCGCTTCCTA TCAGCAGAAG CTGTCGGACA TACTAGGCGC CAAGCCCTCG 
AAGGGAGTAG TTGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sec8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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