Nlg1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlg1
Entrez Gene ID	40913
Full Name	Neuroligin 1
Synonyms	CG12148,CG14596,CG31146,DNlg1,Dmel\CG31146,anon-WO0118547.256,dnlg1,nlg1
General protein information	Preferred Names Neuroligin 1 Names CG31146 gene product from transcript CG31146-RD CG31146-PD CG31146-PE Nlg1-PD Nlg1-PE neuroligin neuroligin 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84D11-84D12

mRNA and Protein(s)

mRNA	Protein	Name
NM_169191.4	NP_731172.2	neuroligin 1, isoform D
NM_001260037.2	NP_001246966.1	neuroligin 1, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic interaction experiments demonstrate Syx4, Syt4, and Nlg1 regulate synaptic growth and plasticity through both shared and parallel signaling pathways.
Title: The postsynaptic t-SNARE Syntaxin 4 controls traffic of Neuroligin 1 and Synaptotagmin 4 to regulate retrograde signaling.

presynaptic spinophilin fine-tunes neurexin/neuroligin signalling to control active zone number and functionality
Title: Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function.

Overexpression of dnlg1 in muscle resulted in synaptic overgrowth, but reduced the amplitudes of excitatory junctional currents.
Title: Drosophila neuroligin 1 regulates synaptic growth and function in response to activity and phosphoinositide-3-kinase.

The following Nlg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04209	NM_169191.4 Latest version!	Drosophila melanogaster neuroligin 1, transcript variant D (Nlg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa04209	NM_001260037.2 Latest version!	Drosophila melanogaster neuroligin 1, transcript variant E (Nlg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlg1 ( NM_169191.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731172.2 Drosophila melanogaster neuroligin 1, transcript variant D (Nlg1), mRNA.

Price & Availability↑

CloneID	OFa04209
Clone ID Related Accession (Same CDS sequence)	NM_169191.4 , NM_001260037.2
Accession Version	NM_169191.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuroligin 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169191.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTC GTTTGGCAGC ATTTTGGCTA TTTCTGCTGA CAGTCGGCGG CAATCACAAG 
CTATTAAGCC ACGTTTCCCC AATGGGCGTG GCAGCCGAAA AGCAGCCGTT GCACAAAGGA 
AATGAGCTGG AAAACGCAAT GAAATTGCGT GAAGCCCCAA AGCAATCCCG GGTTATCGGG 
GACATCACGA CGACAATTCA GCCGGACTCG GATCCGGGCC GATCAATTGG CCATCAGGCG 
TTGCGACGTG CCAAGGCCCC GCCGCCCAGC TTGGAGCTGC ATTTCAGGCA GAATCTGAAC 
AAGCTTTTCG CCGGCGATGA AACCACCCAC GTGGGCCACA CCAATGCCAC TGACAACACC 
ACGGCCGCAG TTTTGGCCAC CGACGAAGGT TCCAGCGAAC ACGAGCCGTC AACCACTCAT 
CACCCGGAAC GGCGACACGT GGTCCCCGAT AAGCTGCAGT ACACCCAGGA GATCCAGGTG 
AAGCAGGGCC GGCTCATGGG CATCACACGT CGCTTCCAGG TGACCAGCGG GCTGCGCCAG 
GTGGACCAGT TCCTGGGGCT TCCATACGCG GAGGCGCCCA CCGGAAATCG ACGCTTCATG 
CCACCGGGTG CTCCACTACC ATGGCAGGGC TTGAAGATAG CGCGACATCT TCCGCCCGTG 
TGTCCGCAGA AATTGCCAGA TCTTTCGCCC CACGGCAGTG AAAATATGTC CCGGGCTCGT 
CATAAACACC TGAGTCGACT GCTCCCTTAT CTGCGGACGG AGAGCGAGGA CTGTTTGTAT 
CTCAATCTGT ATGTGCCACA TGAAGAGCCT CAAAGCACCC CCAAGCAATA CGCTGTGCTG 
GTCTATCTGC ACGGGGAGTC CTTCGAGTGG AACAGTGGCA ATCCCTACGA TGGCTCCGTC 
TTGTCCAGCT ATGGAGAGGT GATTGTGGTC ACCGTCAACT ACCGGCTGGG AGTGTTGGGA 
TTCTTACGGC CGAGCATCGA TGCGCATAAT ATAGCGAATT ACGCCCTGCT CGACCAGATT 
GCGGCGCTAC ACTGGATCAA GGAAAACATC GAAGCCTTCG GCGGTGACAA TTCGCGTGTC 
ACTTTAATGG GGCACAGTAC GGGGGCGGCG TGTGTCAATT ACCTAATGGT ATCGCCGGTG 
GCCTCAGGCC TCTTTCACAG GGCCATCCTC ATGTCCGGAT CCGCGATGTC CGACTGGGCC 
GCCAGCAATC AGTCCCTGCA ATTGACGATG CAAATCGCCC ATGCCCTCGA ATGTCCACTC 
AACGAGCACG TGGAGGCAGA GGACGACGAT GTCCTGCTCG ATTGTTTGCG CCATCGTCGC 
TACCAGGATA TCTTGCACAT ACCAACGGCG CTCACACAAT TTTCAACATC ATTGGGTCCA 
ATTGTCGATG GACATGTAAT ACCAAATCAA CCGTACAAGG TCATGGGGCA TTATACGGAG 
CACTTCAGCC GTTATGATTT ATTATTCGGC ATCACGGAGT CAGAATCCTA TCACACACTG 
GCCGCATTAG CACTCGAGGA AGGATTACGT GAAAATGAAC GCGATAATTT ATTGCGCTTC 
TATATGCAGA GCCGCTTCGA TATACGCCCC GATTTGGCAT TGGCTGCCAC GCTGAAGAAA 
TACCAGGACA TGTACAACAA TCCGATTAAG GCCACTAATT TGGAGCACCG TGACGTGGTT 
CTGGACATAC TGTCTGATGC TCGCGTGGTT GGCCCGCTTC TGCAAACGGG AATGTTCCAC 
GCGGATGTCA ATCGGCGCAA TTATATGTAC GTATTTGGCC ATAACAGCGC GACGGGACCG 
TTTGCCCACC TACCGCATAG CATTATGGGC GAGGAATTGG CGTTTATCTT CGGGGCTCCC 
TTGGCCGCCG CAGGTCCCTT TCCCAGTGGC AACTACACGG TGCAGGAAAA GCTGCTTTCC 
GAGGCGGTAA TGGCATATTG GACGAATTTC GTAAAGACCG GCAATCCCAA GGCCCCATGG 
AAGGGAATCT TCATCAATTC ACATGCCTTG GAGTGGGATC GCTACGACCT GGATTGGCCG 
GAATTCAATA GGCGGGCTCA GGCATATCTC AATATTGGCA TTCCGCCAAC AGTGGGCTAC 
AAGTACCGCC AGATTTATAT GAATTTTTGG AACAAAGAGC TCCCGGACGA ACTCAACCAG 
ATAGCGGCCA TTCAGGAGCA GCTCCAGAAG CCCGGGCAAG AGGTGATTAC AGGTCACATG 
AGCAAGTACG GGCCGAGGGA CCACGGAGCC GAGGACCCTG TGAGGACATT AAAGCTGCTC 
CTCCAGGAAC CCAGTCTAGC TGGACCAACA CAGAGTGGCG AATCGACAGA AACAGCGGCG 
GAAAATATGT ACAATGCTCC ACCCACCTTT GGTCATGTGC ACAAGATGCA GGGTGGTAGT 
GATTTCGAGG ACTTGGTGAC CTCCACCAAC TCACTGGAGA GTGGGGAGGA TCATCCTCAG 
GCTCCTCCTG AAACCGTGGC CAAATCCGAG GCCACGATGC AACTACTGAT CGCCTTAATT 
ACCATTATAA TAGTTCTCAA TCTCCTGATT TATGGAACCT TTTTGTTGCG ACAGCGCCGT 
CGAAGGGCCA AGGCTCTACC TTTTCCGGCT CCTAAATTGG GCGGCACCAT CCTCAGCTAC 
GATGGAGCCA ATGATGAGGA GCTGAAGAGG TGCAGCAAGT CCCGCGACGG AGATGATAGT 
TTCGTCCTGG AGATGACAAG GAAATCAAAT ACCTATGAGG CCATCAAGAC AGGTCAGAGA 
TCGCTGAGTT GCTCTACAGT GGACACCCAC ACTAAGGTCT GTGAGTGGAT GTCGAGTCAG 
GAAGCCCCGA AATCGGGTAG CTTCAACACG GCCACTCCGC CTCCACAACC GATTTTATCC 
GATGGCAGGC TGATCATATG CCAGGATATA GAGGTGGCCG ACGCAGCTCT ACTCATCCCC 
CAACACATGC ACGAGCCGCA GCACTACGAG ATGCTCCTGC AGAGACAACA TTCGGCCCTA 
ACCGAACCGA GTGATGAAAT CCTCCAGCAG CAATATCCCT TGAGGAACCA CTCCCATTCC 
CATTCGGATC CTGTGGACAT GATCCTAGCT GCTGATGAGC AGGTCACCAG CTTTGTGCAT 
GCCGACGATG TGGACATCAA CGTGACAAGC AGAGATGACT CCGATGGTCT GGAGGTTATA 
CCTTTGACGT CTGCTCAGCA ACTAGAGCTT TTGCGTCAGC GTAATTATCC CAAAGTCCTG 
CCCACAGAAC AGGATTTAAT TAACAGCAGC TATAAGAGAA ATTCCCTGCC GCCACAAAAT 
TTCAACGCTC CTCTGCCGCC GCCGCGAACC ATTAGCAACA CTCTGGGCAG GAGGCGGAGG 
GACAGCAGCA ACATAACCAC TTCGCCACTC CAAGTGGCCA GAGATTGTGG AGGCGAGGAC 
GAGGACCTTA AGGAGCCCCA AATCACACAG AACACTCTCA TTGTTGGACC CATTGTGCCC 
AAATCACCCG CCTCCAGTCT CAAGCGAGTG AAGAGAATGC CAGAGTCTTC GGCAATGACG 
GCTCTCAGTG GATCCTTTCA ATCCTTCGAG GCCGTACCCC CGGCCCACGA AACCACGCCC 
CCTCAGGGTG GCGAGCGGAC CGAGTGCATT TACGCAATTA GGCCCAGTCC CGGAAGTTGC 
AGCTGGGCGG CGCCCAATGG CGATTTGTAC GCGCAGCCCA TGAAATCGTC TTCGAGGAAC 
TCGCTCATTC CCAGGCCCAC AAAGCCGGCG GAGTCTCAAT CCCAAGCCAC GCCAGCCGGA 
CCAGCTGGGG AATCTCTTGG ATCAACGGGA ATGGCAACAG CAAGTCGCAT TCCTCAATTG 
CAACGCCAGG CTTCCGGCAA GGATTTGCAA GCAAGAACGG CAACTGACAA CACCGCAAAT 
CCTTTGGGAT GCCAACCGCG AACCGATTCC ACAATATCAT CGGGCTCGTC GGCATCCTAT 
GCCTCGACCA CGGACTCCTC CTCCTCCTCA TCCACTGGAA CCGTACGCAC CGAGCTGCAG 
CAATTCCAGC CAGCTCCCGG CAGAAGTATT ACAACCAATA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731172.2
CDS	768..4832
Translation	MKFRLAAFWLFLLTVGGNHKLLSHVSPMGVAAEKQPLHKGNELENAMKLREAPKQSRVIGDITTTIQPDSDPGRSIGHQALRRAKAPPPSLELHFRQNLNKLFAGDETTHVGHTNATDNTTAAVLATDEGSSEHEPSTTHHPERRHVVPDKLQYTQEIQVKQGRLMGITRRFQVTSGLRQVDQFLGLPYAEAPTGNRRFMPPGAPLPWQGLKIARHLPPVCPQKLPDLSPHGSENMSRARHKHLSRLLPYLRTESEDCLYLNLYVPHEEPQSTPKQYAVLVYLHGESFEWNSGNPYDGSVLSSYGEVIVVTVNYRLGVLGFLRPSIDAHNIANYALLDQIAALHWIKENIEAFGGDNSRVTLMGHSTGAACVNYLMVSPVASGLFHRAILMSGSAMSDWAASNQSLQLTMQIAHALECPLNEHVEAEDDDVLLDCLRHRRYQDILHIPTALTQFSTSLGPIVDGHVIPNQPYKVMGHYTEHFSRYDLLFGITESESYHTLAALALEEGLRENERDNLLRFYMQSRFDIRPDLALAATLKKYQDMYNNPIKATNLEHRDVVLDILSDARVVGPLLQTGMFHADVNRRNYMYVFGHNSATGPFAHLPHSIMGEELAFIFGAPLAAAGPFPSGNYTVQEKLLSEAVMAYWTNFVKTGNPKAPWKGIFINSHALEWDRYDLDWPEFNRRAQAYLNIGIPPTVGYKYRQIYMNFWNKELPDELNQIAAIQEQLQKPGQEVITGHMSKYGPRDHGAEDPVRTLKLLLQEPSLAGPTQSGESTETAAENMYNAPPTFGHVHKMQGGSDFEDLVTSTNSLESGEDHPQAPPETVAKSEATMQLLIALITIIIVLNLLIYGTFLLRQRRRRAKALPFPAPKLGGTILSYDGANDEELKRCSKSRDGDDSFVLEMTRKSNTYEAIKTGQRSLSCSTVDTHTKVCEWMSSQEAPKSGSFNTATPPPQPILSDGRLIICQDIEVADAALLIPQHMHEPQHYEMLLQRQHSALTEPSDEILQQQYPLRNHSHSHSDPVDMILAADEQVTSFVHADDVDINVTSRDDSDGLEVIPLTSAQQLELLRQRNYPKVLPTEQDLINSSYKRNSLPPQNFNAPLPPPRTISNTLGRRRRDSSNITTSPLQVARDCGGEDEDLKEPQITQNTLIVGPIVPKSPASSLKRVKRMPESSAMTALSGSFQSFEAVPPAHETTPPQGGERTECIYAIRPSPGSCSWAAPNGDLYAQPMKSSSRNSLIPRPTKPAESQSQATPAGPAGESLGSTGMATASRIPQLQRQASGKDLQARTATDNTANPLGCQPRTDSTISSGSSASYASTTDSSSSSSTGTVRTELQQFQPAPGRSITTNI

Target ORF information:

RefSeq Version	NM_169191.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuroligin 1, transcript variant D (Nlg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169191.4

ORF Insert Sequence:

ATGAAGTTTC GTTTGGCAGC ATTTTGGCTA TTTCTGCTGA CAGTCGGCGG CAATCACAAG 
CTATTAAGCC ACGTTTCCCC AATGGGCGTG GCAGCCGAAA AGCAGCCGTT GCACAAAGGA 
AATGAGCTGG AAAACGCAAT GAAATTGCGT GAAGCCCCAA AGCAATCCCG GGTTATCGGG 
GACATCACGA CGACAATTCA GCCGGACTCG GATCCGGGCC GATCAATTGG CCATCAGGCG 
TTGCGACGTG CCAAGGCCCC GCCGCCCAGC TTGGAGCTGC ATTTCAGGCA GAATCTGAAC 
AAGCTTTTCG CCGGCGATGA AACCACCCAC GTGGGCCACA CCAATGCCAC TGACAACACC 
ACGGCCGCAG TTTTGGCCAC CGACGAAGGT TCCAGCGAAC ACGAGCCGTC AACCACTCAT 
CACCCGGAAC GGCGACACGT GGTCCCCGAT AAGCTGCAGT ACACCCAGGA GATCCAGGTG 
AAGCAGGGCC GGCTCATGGG CATCACACGT CGCTTCCAGG TGACCAGCGG GCTGCGCCAG 
GTGGACCAGT TCCTGGGGCT TCCATACGCG GAGGCGCCCA CCGGAAATCG ACGCTTCATG 
CCACCGGGTG CTCCACTACC ATGGCAGGGC TTGAAGATAG CGCGACATCT TCCGCCCGTG 
TGTCCGCAGA AATTGCCAGA TCTTTCGCCC CACGGCAGTG AAAATATGTC CCGGGCTCGT 
CATAAACACC TGAGTCGACT GCTCCCTTAT CTGCGGACGG AGAGCGAGGA CTGTTTGTAT 
CTCAATCTGT ATGTGCCACA TGAAGAGCCT CAAAGCACCC CCAAGCAATA CGCTGTGCTG 
GTCTATCTGC ACGGGGAGTC CTTCGAGTGG AACAGTGGCA ATCCCTACGA TGGCTCCGTC 
TTGTCCAGCT ATGGAGAGGT GATTGTGGTC ACCGTCAACT ACCGGCTGGG AGTGTTGGGA 
TTCTTACGGC CGAGCATCGA TGCGCATAAT ATAGCGAATT ACGCCCTGCT CGACCAGATT 
GCGGCGCTAC ACTGGATCAA GGAAAACATC GAAGCCTTCG GCGGTGACAA TTCGCGTGTC 
ACTTTAATGG GGCACAGTAC GGGGGCGGCG TGTGTCAATT ACCTAATGGT ATCGCCGGTG 
GCCTCAGGCC TCTTTCACAG GGCCATCCTC ATGTCCGGAT CCGCGATGTC CGACTGGGCC 
GCCAGCAATC AGTCCCTGCA ATTGACGATG CAAATCGCCC ATGCCCTCGA ATGTCCACTC 
AACGAGCACG TGGAGGCAGA GGACGACGAT GTCCTGCTCG ATTGTTTGCG CCATCGTCGC 
TACCAGGATA TCTTGCACAT ACCAACGGCG CTCACACAAT TTTCAACATC ATTGGGTCCA 
ATTGTCGATG GACATGTAAT ACCAAATCAA CCGTACAAGG TCATGGGGCA TTATACGGAG 
CACTTCAGCC GTTATGATTT ATTATTCGGC ATCACGGAGT CAGAATCCTA TCACACACTG 
GCCGCATTAG CACTCGAGGA AGGATTACGT GAAAATGAAC GCGATAATTT ATTGCGCTTC 
TATATGCAGA GCCGCTTCGA TATACGCCCC GATTTGGCAT TGGCTGCCAC GCTGAAGAAA 
TACCAGGACA TGTACAACAA TCCGATTAAG GCCACTAATT TGGAGCACCG TGACGTGGTT 
CTGGACATAC TGTCTGATGC TCGCGTGGTT GGCCCGCTTC TGCAAACGGG AATGTTCCAC 
GCGGATGTCA ATCGGCGCAA TTATATGTAC GTATTTGGCC ATAACAGCGC GACGGGACCG 
TTTGCCCACC TACCGCATAG CATTATGGGC GAGGAATTGG CGTTTATCTT CGGGGCTCCC 
TTGGCCGCCG CAGGTCCCTT TCCCAGTGGC AACTACACGG TGCAGGAAAA GCTGCTTTCC 
GAGGCGGTAA TGGCATATTG GACGAATTTC GTAAAGACCG GCAATCCCAA GGCCCCATGG 
AAGGGAATCT TCATCAATTC ACATGCCTTG GAGTGGGATC GCTACGACCT GGATTGGCCG 
GAATTCAATA GGCGGGCTCA GGCATATCTC AATATTGGCA TTCCGCCAAC AGTGGGCTAC 
AAGTACCGCC AGATTTATAT GAATTTTTGG AACAAAGAGC TCCCGGACGA ACTCAACCAG 
ATAGCGGCCA TTCAGGAGCA GCTCCAGAAG CCCGGGCAAG AGGTGATTAC AGGTCACATG 
AGCAAGTACG GGCCGAGGGA CCACGGAGCC GAGGACCCTG TGAGGACATT AAAGCTGCTC 
CTCCAGGAAC CCAGTCTAGC TGGACCAACA CAGAGTGGCG AATCGACAGA AACAGCGGCG 
GAAAATATGT ACAATGCTCC ACCCACCTTT GGTCATGTGC ACAAGATGCA GGGTGGTAGT 
GATTTCGAGG ACTTGGTGAC CTCCACCAAC TCACTGGAGA GTGGGGAGGA TCATCCTCAG 
GCTCCTCCTG AAACCGTGGC CAAATCCGAG GCCACGATGC AACTACTGAT CGCCTTAATT 
ACCATTATAA TAGTTCTCAA TCTCCTGATT TATGGAACCT TTTTGTTGCG ACAGCGCCGT 
CGAAGGGCCA AGGCTCTACC TTTTCCGGCT CCTAAATTGG GCGGCACCAT CCTCAGCTAC 
GATGGAGCCA ATGATGAGGA GCTGAAGAGG TGCAGCAAGT CCCGCGACGG AGATGATAGT 
TTCGTCCTGG AGATGACAAG GAAATCAAAT ACCTATGAGG CCATCAAGAC AGGTCAGAGA 
TCGCTGAGTT GCTCTACAGT GGACACCCAC ACTAAGGTCT GTGAGTGGAT GTCGAGTCAG 
GAAGCCCCGA AATCGGGTAG CTTCAACACG GCCACTCCGC CTCCACAACC GATTTTATCC 
GATGGCAGGC TGATCATATG CCAGGATATA GAGGTGGCCG ACGCAGCTCT ACTCATCCCC 
CAACACATGC ACGAGCCGCA GCACTACGAG ATGCTCCTGC AGAGACAACA TTCGGCCCTA 
ACCGAACCGA GTGATGAAAT CCTCCAGCAG CAATATCCCT TGAGGAACCA CTCCCATTCC 
CATTCGGATC CTGTGGACAT GATCCTAGCT GCTGATGAGC AGGTCACCAG CTTTGTGCAT 
GCCGACGATG TGGACATCAA CGTGACAAGC AGAGATGACT CCGATGGTCT GGAGGTTATA 
CCTTTGACGT CTGCTCAGCA ACTAGAGCTT TTGCGTCAGC GTAATTATCC CAAAGTCCTG 
CCCACAGAAC AGGATTTAAT TAACAGCAGC TATAAGAGAA ATTCCCTGCC GCCACAAAAT 
TTCAACGCTC CTCTGCCGCC GCCGCGAACC ATTAGCAACA CTCTGGGCAG GAGGCGGAGG 
GACAGCAGCA ACATAACCAC TTCGCCACTC CAAGTGGCCA GAGATTGTGG AGGCGAGGAC 
GAGGACCTTA AGGAGCCCCA AATCACACAG AACACTCTCA TTGTTGGACC CATTGTGCCC 
AAATCACCCG CCTCCAGTCT CAAGCGAGTG AAGAGAATGC CAGAGTCTTC GGCAATGACG 
GCTCTCAGTG GATCCTTTCA ATCCTTCGAG GCCGTACCCC CGGCCCACGA AACCACGCCC 
CCTCAGGGTG GCGAGCGGAC CGAGTGCATT TACGCAATTA GGCCCAGTCC CGGAAGTTGC 
AGCTGGGCGG CGCCCAATGG CGATTTGTAC GCGCAGCCCA TGAAATCGTC TTCGAGGAAC 
TCGCTCATTC CCAGGCCCAC AAAGCCGGCG GAGTCTCAAT CCCAAGCCAC GCCAGCCGGA 
CCAGCTGGGG AATCTCTTGG ATCAACGGGA ATGGCAACAG CAAGTCGCAT TCCTCAATTG 
CAACGCCAGG CTTCCGGCAA GGATTTGCAA GCAAGAACGG CAACTGACAA CACCGCAAAT 
CCTTTGGGAT GCCAACCGCG AACCGATTCC ACAATATCAT CGGGCTCGTC GGCATCCTAT 
GCCTCGACCA CGGACTCCTC CTCCTCCTCA TCCACTGGAA CCGTACGCAC CGAGCTGCAG 
CAATTCCAGC CAGCTCCCGG CAGAAGTATT ACAACCAATA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlg1 ( NM_001260037.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246966.1 Drosophila melanogaster neuroligin 1, transcript variant E (Nlg1), mRNA.

Price & Availability↑

CloneID	OFa04209
Clone ID Related Accession (Same CDS sequence)	NM_169191.4 , NM_001260037.2
Accession Version	NM_001260037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuroligin 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260037.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTTC GTTTGGCAGC ATTTTGGCTA TTTCTGCTGA CAGTCGGCGG CAATCACAAG 
CTATTAAGCC ACGTTTCCCC AATGGGCGTG GCAGCCGAAA AGCAGCCGTT GCACAAAGGA 
AATGAGCTGG AAAACGCAAT GAAATTGCGT GAAGCCCCAA AGCAATCCCG GGTTATCGGG 
GACATCACGA CGACAATTCA GCCGGACTCG GATCCGGGCC GATCAATTGG CCATCAGGCG 
TTGCGACGTG CCAAGGCCCC GCCGCCCAGC TTGGAGCTGC ATTTCAGGCA GAATCTGAAC 
AAGCTTTTCG CCGGCGATGA AACCACCCAC GTGGGCCACA CCAATGCCAC TGACAACACC 
ACGGCCGCAG TTTTGGCCAC CGACGAAGGT TCCAGCGAAC ACGAGCCGTC AACCACTCAT 
CACCCGGAAC GGCGACACGT GGTCCCCGAT AAGCTGCAGT ACACCCAGGA GATCCAGGTG 
AAGCAGGGCC GGCTCATGGG CATCACACGT CGCTTCCAGG TGACCAGCGG GCTGCGCCAG 
GTGGACCAGT TCCTGGGGCT TCCATACGCG GAGGCGCCCA CCGGAAATCG ACGCTTCATG 
CCACCGGGTG CTCCACTACC ATGGCAGGGC TTGAAGATAG CGCGACATCT TCCGCCCGTG 
TGTCCGCAGA AATTGCCAGA TCTTTCGCCC CACGGCAGTG AAAATATGTC CCGGGCTCGT 
CATAAACACC TGAGTCGACT GCTCCCTTAT CTGCGGACGG AGAGCGAGGA CTGTTTGTAT 
CTCAATCTGT ATGTGCCACA TGAAGAGCCT CAAAGCACCC CCAAGCAATA CGCTGTGCTG 
GTCTATCTGC ACGGGGAGTC CTTCGAGTGG AACAGTGGCA ATCCCTACGA TGGCTCCGTC 
TTGTCCAGCT ATGGAGAGGT GATTGTGGTC ACCGTCAACT ACCGGCTGGG AGTGTTGGGA 
TTCTTACGGC CGAGCATCGA TGCGCATAAT ATAGCGAATT ACGCCCTGCT CGACCAGATT 
GCGGCGCTAC ACTGGATCAA GGAAAACATC GAAGCCTTCG GCGGTGACAA TTCGCGTGTC 
ACTTTAATGG GGCACAGTAC GGGGGCGGCG TGTGTCAATT ACCTAATGGT ATCGCCGGTG 
GCCTCAGGCC TCTTTCACAG GGCCATCCTC ATGTCCGGAT CCGCGATGTC CGACTGGGCC 
GCCAGCAATC AGTCCCTGCA ATTGACGATG CAAATCGCCC ATGCCCTCGA ATGTCCACTC 
AACGAGCACG TGGAGGCAGA GGACGACGAT GTCCTGCTCG ATTGTTTGCG CCATCGTCGC 
TACCAGGATA TCTTGCACAT ACCAACGGCG CTCACACAAT TTTCAACATC ATTGGGTCCA 
ATTGTCGATG GACATGTAAT ACCAAATCAA CCGTACAAGG TCATGGGGCA TTATACGGAG 
CACTTCAGCC GTTATGATTT ATTATTCGGC ATCACGGAGT CAGAATCCTA TCACACACTG 
GCCGCATTAG CACTCGAGGA AGGATTACGT GAAAATGAAC GCGATAATTT ATTGCGCTTC 
TATATGCAGA GCCGCTTCGA TATACGCCCC GATTTGGCAT TGGCTGCCAC GCTGAAGAAA 
TACCAGGACA TGTACAACAA TCCGATTAAG GCCACTAATT TGGAGCACCG TGACGTGGTT 
CTGGACATAC TGTCTGATGC TCGCGTGGTT GGCCCGCTTC TGCAAACGGG AATGTTCCAC 
GCGGATGTCA ATCGGCGCAA TTATATGTAC GTATTTGGCC ATAACAGCGC GACGGGACCG 
TTTGCCCACC TACCGCATAG CATTATGGGC GAGGAATTGG CGTTTATCTT CGGGGCTCCC 
TTGGCCGCCG CAGGTCCCTT TCCCAGTGGC AACTACACGG TGCAGGAAAA GCTGCTTTCC 
GAGGCGGTAA TGGCATATTG GACGAATTTC GTAAAGACCG GCAATCCCAA GGCCCCATGG 
AAGGGAATCT TCATCAATTC ACATGCCTTG GAGTGGGATC GCTACGACCT GGATTGGCCG 
GAATTCAATA GGCGGGCTCA GGCATATCTC AATATTGGCA TTCCGCCAAC AGTGGGCTAC 
AAGTACCGCC AGATTTATAT GAATTTTTGG AACAAAGAGC TCCCGGACGA ACTCAACCAG 
ATAGCGGCCA TTCAGGAGCA GCTCCAGAAG CCCGGGCAAG AGGTGATTAC AGGTCACATG 
AGCAAGTACG GGCCGAGGGA CCACGGAGCC GAGGACCCTG TGAGGACATT AAAGCTGCTC 
CTCCAGGAAC CCAGTCTAGC TGGACCAACA CAGAGTGGCG AATCGACAGA AACAGCGGCG 
GAAAATATGT ACAATGCTCC ACCCACCTTT GGTCATGTGC ACAAGATGCA GGGTGGTAGT 
GATTTCGAGG ACTTGGTGAC CTCCACCAAC TCACTGGAGA GTGGGGAGGA TCATCCTCAG 
GCTCCTCCTG AAACCGTGGC CAAATCCGAG GCCACGATGC AACTACTGAT CGCCTTAATT 
ACCATTATAA TAGTTCTCAA TCTCCTGATT TATGGAACCT TTTTGTTGCG ACAGCGCCGT 
CGAAGGGCCA AGGCTCTACC TTTTCCGGCT CCTAAATTGG GCGGCACCAT CCTCAGCTAC 
GATGGAGCCA ATGATGAGGA GCTGAAGAGG TGCAGCAAGT CCCGCGACGG AGATGATAGT 
TTCGTCCTGG AGATGACAAG GAAATCAAAT ACCTATGAGG CCATCAAGAC AGGTCAGAGA 
TCGCTGAGTT GCTCTACAGT GGACACCCAC ACTAAGGTCT GTGAGTGGAT GTCGAGTCAG 
GAAGCCCCGA AATCGGGTAG CTTCAACACG GCCACTCCGC CTCCACAACC GATTTTATCC 
GATGGCAGGC TGATCATATG CCAGGATATA GAGGTGGCCG ACGCAGCTCT ACTCATCCCC 
CAACACATGC ACGAGCCGCA GCACTACGAG ATGCTCCTGC AGAGACAACA TTCGGCCCTA 
ACCGAACCGA GTGATGAAAT CCTCCAGCAG CAATATCCCT TGAGGAACCA CTCCCATTCC 
CATTCGGATC CTGTGGACAT GATCCTAGCT GCTGATGAGC AGGTCACCAG CTTTGTGCAT 
GCCGACGATG TGGACATCAA CGTGACAAGC AGAGATGACT CCGATGGTCT GGAGGTTATA 
CCTTTGACGT CTGCTCAGCA ACTAGAGCTT TTGCGTCAGC GTAATTATCC CAAAGTCCTG 
CCCACAGAAC AGGATTTAAT TAACAGCAGC TATAAGAGAA ATTCCCTGCC GCCACAAAAT 
TTCAACGCTC CTCTGCCGCC GCCGCGAACC ATTAGCAACA CTCTGGGCAG GAGGCGGAGG 
GACAGCAGCA ACATAACCAC TTCGCCACTC CAAGTGGCCA GAGATTGTGG AGGCGAGGAC 
GAGGACCTTA AGGAGCCCCA AATCACACAG AACACTCTCA TTGTTGGACC CATTGTGCCC 
AAATCACCCG CCTCCAGTCT CAAGCGAGTG AAGAGAATGC CAGAGTCTTC GGCAATGACG 
GCTCTCAGTG GATCCTTTCA ATCCTTCGAG GCCGTACCCC CGGCCCACGA AACCACGCCC 
CCTCAGGGTG GCGAGCGGAC CGAGTGCATT TACGCAATTA GGCCCAGTCC CGGAAGTTGC 
AGCTGGGCGG CGCCCAATGG CGATTTGTAC GCGCAGCCCA TGAAATCGTC TTCGAGGAAC 
TCGCTCATTC CCAGGCCCAC AAAGCCGGCG GAGTCTCAAT CCCAAGCCAC GCCAGCCGGA 
CCAGCTGGGG AATCTCTTGG ATCAACGGGA ATGGCAACAG CAAGTCGCAT TCCTCAATTG 
CAACGCCAGG CTTCCGGCAA GGATTTGCAA GCAAGAACGG CAACTGACAA CACCGCAAAT 
CCTTTGGGAT GCCAACCGCG AACCGATTCC ACAATATCAT CGGGCTCGTC GGCATCCTAT 
GCCTCGACCA CGGACTCCTC CTCCTCCTCA TCCACTGGAA CCGTACGCAC CGAGCTGCAG 
CAATTCCAGC CAGCTCCCGG CAGAAGTATT ACAACCAATA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246966.1
CDS	453..4517
Translation	MKFRLAAFWLFLLTVGGNHKLLSHVSPMGVAAEKQPLHKGNELENAMKLREAPKQSRVIGDITTTIQPDSDPGRSIGHQALRRAKAPPPSLELHFRQNLNKLFAGDETTHVGHTNATDNTTAAVLATDEGSSEHEPSTTHHPERRHVVPDKLQYTQEIQVKQGRLMGITRRFQVTSGLRQVDQFLGLPYAEAPTGNRRFMPPGAPLPWQGLKIARHLPPVCPQKLPDLSPHGSENMSRARHKHLSRLLPYLRTESEDCLYLNLYVPHEEPQSTPKQYAVLVYLHGESFEWNSGNPYDGSVLSSYGEVIVVTVNYRLGVLGFLRPSIDAHNIANYALLDQIAALHWIKENIEAFGGDNSRVTLMGHSTGAACVNYLMVSPVASGLFHRAILMSGSAMSDWAASNQSLQLTMQIAHALECPLNEHVEAEDDDVLLDCLRHRRYQDILHIPTALTQFSTSLGPIVDGHVIPNQPYKVMGHYTEHFSRYDLLFGITESESYHTLAALALEEGLRENERDNLLRFYMQSRFDIRPDLALAATLKKYQDMYNNPIKATNLEHRDVVLDILSDARVVGPLLQTGMFHADVNRRNYMYVFGHNSATGPFAHLPHSIMGEELAFIFGAPLAAAGPFPSGNYTVQEKLLSEAVMAYWTNFVKTGNPKAPWKGIFINSHALEWDRYDLDWPEFNRRAQAYLNIGIPPTVGYKYRQIYMNFWNKELPDELNQIAAIQEQLQKPGQEVITGHMSKYGPRDHGAEDPVRTLKLLLQEPSLAGPTQSGESTETAAENMYNAPPTFGHVHKMQGGSDFEDLVTSTNSLESGEDHPQAPPETVAKSEATMQLLIALITIIIVLNLLIYGTFLLRQRRRRAKALPFPAPKLGGTILSYDGANDEELKRCSKSRDGDDSFVLEMTRKSNTYEAIKTGQRSLSCSTVDTHTKVCEWMSSQEAPKSGSFNTATPPPQPILSDGRLIICQDIEVADAALLIPQHMHEPQHYEMLLQRQHSALTEPSDEILQQQYPLRNHSHSHSDPVDMILAADEQVTSFVHADDVDINVTSRDDSDGLEVIPLTSAQQLELLRQRNYPKVLPTEQDLINSSYKRNSLPPQNFNAPLPPPRTISNTLGRRRRDSSNITTSPLQVARDCGGEDEDLKEPQITQNTLIVGPIVPKSPASSLKRVKRMPESSAMTALSGSFQSFEAVPPAHETTPPQGGERTECIYAIRPSPGSCSWAAPNGDLYAQPMKSSSRNSLIPRPTKPAESQSQATPAGPAGESLGSTGMATASRIPQLQRQASGKDLQARTATDNTANPLGCQPRTDSTISSGSSASYASTTDSSSSSSTGTVRTELQQFQPAPGRSITTNI

Target ORF information:

RefSeq Version	NM_001260037.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuroligin 1, transcript variant E (Nlg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260037.2

ORF Insert Sequence:

ATGAAGTTTC GTTTGGCAGC ATTTTGGCTA TTTCTGCTGA CAGTCGGCGG CAATCACAAG 
CTATTAAGCC ACGTTTCCCC AATGGGCGTG GCAGCCGAAA AGCAGCCGTT GCACAAAGGA 
AATGAGCTGG AAAACGCAAT GAAATTGCGT GAAGCCCCAA AGCAATCCCG GGTTATCGGG 
GACATCACGA CGACAATTCA GCCGGACTCG GATCCGGGCC GATCAATTGG CCATCAGGCG 
TTGCGACGTG CCAAGGCCCC GCCGCCCAGC TTGGAGCTGC ATTTCAGGCA GAATCTGAAC 
AAGCTTTTCG CCGGCGATGA AACCACCCAC GTGGGCCACA CCAATGCCAC TGACAACACC 
ACGGCCGCAG TTTTGGCCAC CGACGAAGGT TCCAGCGAAC ACGAGCCGTC AACCACTCAT 
CACCCGGAAC GGCGACACGT GGTCCCCGAT AAGCTGCAGT ACACCCAGGA GATCCAGGTG 
AAGCAGGGCC GGCTCATGGG CATCACACGT CGCTTCCAGG TGACCAGCGG GCTGCGCCAG 
GTGGACCAGT TCCTGGGGCT TCCATACGCG GAGGCGCCCA CCGGAAATCG ACGCTTCATG 
CCACCGGGTG CTCCACTACC ATGGCAGGGC TTGAAGATAG CGCGACATCT TCCGCCCGTG 
TGTCCGCAGA AATTGCCAGA TCTTTCGCCC CACGGCAGTG AAAATATGTC CCGGGCTCGT 
CATAAACACC TGAGTCGACT GCTCCCTTAT CTGCGGACGG AGAGCGAGGA CTGTTTGTAT 
CTCAATCTGT ATGTGCCACA TGAAGAGCCT CAAAGCACCC CCAAGCAATA CGCTGTGCTG 
GTCTATCTGC ACGGGGAGTC CTTCGAGTGG AACAGTGGCA ATCCCTACGA TGGCTCCGTC 
TTGTCCAGCT ATGGAGAGGT GATTGTGGTC ACCGTCAACT ACCGGCTGGG AGTGTTGGGA 
TTCTTACGGC CGAGCATCGA TGCGCATAAT ATAGCGAATT ACGCCCTGCT CGACCAGATT 
GCGGCGCTAC ACTGGATCAA GGAAAACATC GAAGCCTTCG GCGGTGACAA TTCGCGTGTC 
ACTTTAATGG GGCACAGTAC GGGGGCGGCG TGTGTCAATT ACCTAATGGT ATCGCCGGTG 
GCCTCAGGCC TCTTTCACAG GGCCATCCTC ATGTCCGGAT CCGCGATGTC CGACTGGGCC 
GCCAGCAATC AGTCCCTGCA ATTGACGATG CAAATCGCCC ATGCCCTCGA ATGTCCACTC 
AACGAGCACG TGGAGGCAGA GGACGACGAT GTCCTGCTCG ATTGTTTGCG CCATCGTCGC 
TACCAGGATA TCTTGCACAT ACCAACGGCG CTCACACAAT TTTCAACATC ATTGGGTCCA 
ATTGTCGATG GACATGTAAT ACCAAATCAA CCGTACAAGG TCATGGGGCA TTATACGGAG 
CACTTCAGCC GTTATGATTT ATTATTCGGC ATCACGGAGT CAGAATCCTA TCACACACTG 
GCCGCATTAG CACTCGAGGA AGGATTACGT GAAAATGAAC GCGATAATTT ATTGCGCTTC 
TATATGCAGA GCCGCTTCGA TATACGCCCC GATTTGGCAT TGGCTGCCAC GCTGAAGAAA 
TACCAGGACA TGTACAACAA TCCGATTAAG GCCACTAATT TGGAGCACCG TGACGTGGTT 
CTGGACATAC TGTCTGATGC TCGCGTGGTT GGCCCGCTTC TGCAAACGGG AATGTTCCAC 
GCGGATGTCA ATCGGCGCAA TTATATGTAC GTATTTGGCC ATAACAGCGC GACGGGACCG 
TTTGCCCACC TACCGCATAG CATTATGGGC GAGGAATTGG CGTTTATCTT CGGGGCTCCC 
TTGGCCGCCG CAGGTCCCTT TCCCAGTGGC AACTACACGG TGCAGGAAAA GCTGCTTTCC 
GAGGCGGTAA TGGCATATTG GACGAATTTC GTAAAGACCG GCAATCCCAA GGCCCCATGG 
AAGGGAATCT TCATCAATTC ACATGCCTTG GAGTGGGATC GCTACGACCT GGATTGGCCG 
GAATTCAATA GGCGGGCTCA GGCATATCTC AATATTGGCA TTCCGCCAAC AGTGGGCTAC 
AAGTACCGCC AGATTTATAT GAATTTTTGG AACAAAGAGC TCCCGGACGA ACTCAACCAG 
ATAGCGGCCA TTCAGGAGCA GCTCCAGAAG CCCGGGCAAG AGGTGATTAC AGGTCACATG 
AGCAAGTACG GGCCGAGGGA CCACGGAGCC GAGGACCCTG TGAGGACATT AAAGCTGCTC 
CTCCAGGAAC CCAGTCTAGC TGGACCAACA CAGAGTGGCG AATCGACAGA AACAGCGGCG 
GAAAATATGT ACAATGCTCC ACCCACCTTT GGTCATGTGC ACAAGATGCA GGGTGGTAGT 
GATTTCGAGG ACTTGGTGAC CTCCACCAAC TCACTGGAGA GTGGGGAGGA TCATCCTCAG 
GCTCCTCCTG AAACCGTGGC CAAATCCGAG GCCACGATGC AACTACTGAT CGCCTTAATT 
ACCATTATAA TAGTTCTCAA TCTCCTGATT TATGGAACCT TTTTGTTGCG ACAGCGCCGT 
CGAAGGGCCA AGGCTCTACC TTTTCCGGCT CCTAAATTGG GCGGCACCAT CCTCAGCTAC 
GATGGAGCCA ATGATGAGGA GCTGAAGAGG TGCAGCAAGT CCCGCGACGG AGATGATAGT 
TTCGTCCTGG AGATGACAAG GAAATCAAAT ACCTATGAGG CCATCAAGAC AGGTCAGAGA 
TCGCTGAGTT GCTCTACAGT GGACACCCAC ACTAAGGTCT GTGAGTGGAT GTCGAGTCAG 
GAAGCCCCGA AATCGGGTAG CTTCAACACG GCCACTCCGC CTCCACAACC GATTTTATCC 
GATGGCAGGC TGATCATATG CCAGGATATA GAGGTGGCCG ACGCAGCTCT ACTCATCCCC 
CAACACATGC ACGAGCCGCA GCACTACGAG ATGCTCCTGC AGAGACAACA TTCGGCCCTA 
ACCGAACCGA GTGATGAAAT CCTCCAGCAG CAATATCCCT TGAGGAACCA CTCCCATTCC 
CATTCGGATC CTGTGGACAT GATCCTAGCT GCTGATGAGC AGGTCACCAG CTTTGTGCAT 
GCCGACGATG TGGACATCAA CGTGACAAGC AGAGATGACT CCGATGGTCT GGAGGTTATA 
CCTTTGACGT CTGCTCAGCA ACTAGAGCTT TTGCGTCAGC GTAATTATCC CAAAGTCCTG 
CCCACAGAAC AGGATTTAAT TAACAGCAGC TATAAGAGAA ATTCCCTGCC GCCACAAAAT 
TTCAACGCTC CTCTGCCGCC GCCGCGAACC ATTAGCAACA CTCTGGGCAG GAGGCGGAGG 
GACAGCAGCA ACATAACCAC TTCGCCACTC CAAGTGGCCA GAGATTGTGG AGGCGAGGAC 
GAGGACCTTA AGGAGCCCCA AATCACACAG AACACTCTCA TTGTTGGACC CATTGTGCCC 
AAATCACCCG CCTCCAGTCT CAAGCGAGTG AAGAGAATGC CAGAGTCTTC GGCAATGACG 
GCTCTCAGTG GATCCTTTCA ATCCTTCGAG GCCGTACCCC CGGCCCACGA AACCACGCCC 
CCTCAGGGTG GCGAGCGGAC CGAGTGCATT TACGCAATTA GGCCCAGTCC CGGAAGTTGC 
AGCTGGGCGG CGCCCAATGG CGATTTGTAC GCGCAGCCCA TGAAATCGTC TTCGAGGAAC 
TCGCTCATTC CCAGGCCCAC AAAGCCGGCG GAGTCTCAAT CCCAAGCCAC GCCAGCCGGA 
CCAGCTGGGG AATCTCTTGG ATCAACGGGA ATGGCAACAG CAAGTCGCAT TCCTCAATTG 
CAACGCCAGG CTTCCGGCAA GGATTTGCAA GCAAGAACGG CAACTGACAA CACCGCAAAT 
CCTTTGGGAT GCCAACCGCG AACCGATTCC ACAATATCAT CGGGCTCGTC GGCATCCTAT 
GCCTCGACCA CGGACTCCTC CTCCTCCTCA TCCACTGGAA CCGTACGCAC CGAGCTGCAG 
CAATTCCAGC CAGCTCCCGG CAGAAGTATT ACAACCAATA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nlg1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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