2mit cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	2mit
Entrez Gene ID	41616
Full Name	CG42573 gene product from transcript CG42573-RA
Synonyms	CG10148,CG17319,CG42573,CT28541,CT38314,Dmel\CG42573,Dmel_CG10148,Dmel_CG17319
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87D13-87D13

mRNA and Protein(s)

mRNA	Protein	Name
NM_142001.4	NP_650258.2	2mit, isoform A
NM_001275600.2	NP_001262529.1	2mit, isoform B
NM_001300381.1	NP_001287310.1	2mit, isoform C
NM_001300382.1	NP_001287311.1	2mit, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

2mit, an intronic gene of Drosophila melanogaster timeless2, is involved in behavioral plasticity.
Title: 2mit, an intronic gene of Drosophila melanogaster timeless2, is involved in behavioral plasticity.

The following 2mit gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the 2mit cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04769	NM_001300382.1 Latest version!	Drosophila melanogaster 2mit, transcript variant D (2mit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa04769	NM_001300381.1 Latest version!	Drosophila melanogaster 2mit, transcript variant C (2mit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa04769	NM_142001.4 Latest version!	Drosophila melanogaster 2mit, transcript variant A (2mit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa07720	NM_001275600.2 Latest version!	Drosophila melanogaster 2mit, transcript variant B (2mit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

2mit ( NM_142001.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650258.2 Drosophila melanogaster 2mit, transcript variant A (2mit), mRNA.

Price & Availability↑

CloneID	OFa04769
Clone ID Related Accession (Same CDS sequence)	NM_142001.4 , NM_001300381.1 , NM_001300382.1
Accession Version	NM_142001.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	2mit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142001.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAAAA TCGTGAACCA ACACTGTGAT CTAGGCCTGG CCCTTCTCCT GGCCTGGACG 
TGGCTAACAA GGCTGGTGGT GGCGGCCCAT CTGGTCGACA TCCCCACCTC ATCACGCCTG 
GCCGCTGAAC GGGAGGAGCA GCAGTTGTCC CGGCAGGACG TAGGACGGCT CAGCTACCAG 
AGTATCCATC GTATGCTGAG GGACGAAAAC GAACCGGATA GCTTTCGGGG GGAATTGCGA 
TACCAACAGA AACGCCACAA AAGGGAGCTG GAGCTGAATG CGCCGGCCAA CAAGCTTAAT 
CTCACCCACC GCGATTTGAG GACATTCAAT AGCACTGGTG GTCAGTGGAA GGGCGACTTT 
CAAGTGATCA CCGCCATGGA TCTGAGCAGC AATCAACTGG AGAGCCTCAG CTTGGACAAC 
TTCAATCAAC TGAGGCAGCT GGACTTGGGA AATAACTCCC TGGAGGTAAT ACCCTTGAGT 
TTGGCAGACA CCAATATGTC ACTACCCTTT GTGACGCTCG ATCTTTCCTG CAACAAATTC 
AGCCAAATTT CTACGAGCTT TTTTGCCCAG CGATTGCCTC AGTTGAAAAA TCTGAATCTG 
GCTCACAATG AATTGCTAAA TATTTCCCGG GAATCATTCT ATAATTTATT GGAACTACAA 
ACGTTAGTTC TCAGTCACAA CAACATCTCG GATATTGACT ATGAAACATT TTTGGCACTA 
CCGAATCTGC AATATCTGGA TTTATCCCAT AACCGCTTGA GTGGATCCGC TATTCGTGCT 
CTGCAAGGAA TTCCGGATTT GGTCAGCCTT TCCATCGCTT ACAATCCAGA TGTGGGAGTG 
GCGATGCAGG AGTTCGTTGC TTCCTGGAGC CTAAAGGAGT TGGATGCCAG TGGCACTGGA 
TTGTGTCAGG TGCCTGCAGC TCTAGCCCAA TCCGTGAGGA CTCTCAAGTT GTCCGACAAC 
TGGCTCAAGG CAATTAATTG CGGTGACATG GACAGCTATC CGCTGCTGCA GTATCTTGAT 
CTCTCGCACT CCCGCATTGC CCAAGTGGAG GACGATGCCT TGGGACGATT GGAGCTCCTC 
GAATCCCTTT TCCTAGACCG TAATCTACTG ATGCGAGTGC CAAGTAGTCT GCCGCCATCG 
CTGGAACACT TATTTCTGCA GCACAATCAG ATAATGGAGC TTCCGCCACA GGCTTTTGTG 
GGATTGGTCA ATCTACAGAC TCTGGACTTA TCCAACAATC GATTGATCTT CCTGCCCCCG 
CTATCGCTCC CCAAATTGCT CACCCTGAAT CTGGAATCGT CAGGGGTGGA GAGCGTTAGC 
CAATCGATAG TGCACACACT GCCACAGTTA AGGGATCTTC TACTGGAGGA CAACCCCATT 
AAGTGCAGCG ATTTGCTGGG CATTGCCGAA TGGGCCAGTC CTTGCAGGTC AGTGGATGCG 
GGTCAATCGA ATGGGGCAAG TGTAAGTGGG CGCGTGGATT TGAAGACGGA GTATCTGCAA 
TTCCACAATT TTTACGAAAA CTTCAGCAGC CGAGAGTGTG GTATAAGAAA ACCGGAAAAT 
GACACAAAGC CGCCTTCTTG CAGCCTAACA AGGGCATCAG CTACATTAAC AACAACACCC 
AGAAGTATGT CAAAAGTTGA AAAGTCACAA GAAGCACAAG CAACAGCAAC ATCAGTGGAA 
GTAGCAGCGG CAACAGCAGC AACATCAGAA AAAACAGGCA TACAAGCAAC ATCAGCAGCA 
CAGTCGACTG CGGCGGCAAC AACAGCAGAA AGAGAAGCAA TTGCAACAGC AACATCAAGC 
GACACCACAG CAACACCAAC ACTCGCAGCA GCAGCAGCAA TACAATCAGC TGGCAACATC 
CCTGCCCAGT TAACCACAAA AACGTTGCGG CCCACAGAAA CAACTTCGTT AGCGCAACTG 
CAGCGTCGGC AACAAATGCC TGGCATGCCG GCCAAAACAA CAGAAACGCC AGCAAAGAAC 
CTGCCAAGTT TGGCCCAGAC AAAGGCAACA ACAGCGCTTC CCATTTTGGC AACACGAGAT 
GCTGCCACAG CAACAACGGA AATAAATTCC GACAAGCCAA CGAACATTAG CGGTGCCACA 
AAAACAGTAG CAACATCAGC TGCAGAAATA GCAACACCAC CAGCAATTGA AGTGCCACAA 
ACCATTTTGG CCGGCAAAAA ATCTGACAAA ATGCCAGCCG ATAAGGCACA CGAGACTTTA 
TTAAAATACC CAACAAGGGA CACATCCGGT CAAGTTGCAA CAACGCCACA CAAACATGCA 
ACACTGCAGC TGCACGTTAA GGATCGACAT CTAATTGGCA CACCGCTGCT GATGCACAAG 
GGCGATGTCC TATTGGTGGA TGCCGAGCAG TTGTTGCTGC CTGGTACGGC CACCGTGGCG 
GATGCGGATT CGGAAGTTCT GGACCCGAGC CAACAACATC AGTCAGCGGA GCAGGAAAAG 
CACCAGTCAG CGACTGATAA GCGGCAAGCG GATGCAATAA ACGGCGACAC GAAGTCGCCG 
GCGAAAGGCC ACAAAAAGAA ACCATCGCTG AGCATCAAGA AGATGACCTA CAGTACCAAA 
CACGCGGCAA AAACCGTGGA GGATATGGCA GCCACCTCGA AGACACCGCA ACACCAACAT 
AGCAGTGTGA ACACACCCAA AGAGGCTGCT CCCGAGGAGT TGAGCACCTT TGCGCAGCTT 
AAAGCCTATG TGGAGCTAAA GAGCGAATCG AAACCGGAAC ACCTAATGGA CCAGCGGGAG 
GAAAACCATC ATAATCTTAC AGGAAATCAT CCAGGAGTCA TGCTCCTGGT GGCCTGCGTT 
TTGTTCATCG TCCTGCTCGC CGGTTTGGCC CACGTCTATC GCTGTGAATT GCCTTGGCAA 
AGGAGCAACC GCTCTGGTCA ATTGCGACCG CATCATCAAA GACACCTAAA CGAAACCGAT 
GATGCGCACA GCTTCCTGCA CTATCAGGGA TCTGTGAACT CCAATGGCGG TGATCCGGCT 
CGCCTGCAAA AGTGGCACCA CAGCACGCGG AGAGAAGCAC CCTACAGCTC CCCGCTGCAC 
AATCTACAAG CGCGGGAACT GCAACAGCAA CGCTGCCAGC AATTCTATAG CTCCTCGCTG 
GCGGACAAGA GCTCCTCCAC CTCGTCTTCC TCGTCGGGAA GCAGTCGCAG TAGCCTGCAC 
TCGCCCAGCA GAGATGACAG CTACTATATA GAGATGGCGC CCAGTAGTCC ACCAGCAGCC 
AACCTGCCCA GTTTGCCCAT GGAACTTTTG GGCAGCCGGA GTAATGCCCT CGGATGCCGG 
ACGGATCGAG TGGCAGCCAC CGATCTGGGC GGTACGACAG AGGCGGTACC CTCTTCGGCG 
GCGGCCATCA AGTCGGTGAG CAGCAGACTG ATGACGCCCA GTTCGCGGAG GCTGAACATC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650258.2
CDS	1320..4745
Translation	MVKIVNQHCDLGLALLLAWTWLTRLVVAAHLVDIPTSSRLAAEREEQQLSRQDVGRLSYQSIHRMLRDENEPDSFRGELRYQQKRHKRELELNAPANKLNLTHRDLRTFNSTGGQWKGDFQVITAMDLSSNQLESLSLDNFNQLRQLDLGNNSLEVIPLSLADTNMSLPFVTLDLSCNKFSQISTSFFAQRLPQLKNLNLAHNELLNISRESFYNLLELQTLVLSHNNISDIDYETFLALPNLQYLDLSHNRLSGSAIRALQGIPDLVSLSIAYNPDVGVAMQEFVASWSLKELDASGTGLCQVPAALAQSVRTLKLSDNWLKAINCGDMDSYPLLQYLDLSHSRIAQVEDDALGRLELLESLFLDRNLLMRVPSSLPPSLEHLFLQHNQIMELPPQAFVGLVNLQTLDLSNNRLIFLPPLSLPKLLTLNLESSGVESVSQSIVHTLPQLRDLLLEDNPIKCSDLLGIAEWASPCRSVDAGQSNGASVSGRVDLKTEYLQFHNFYENFSSRECGIRKPENDTKPPSCSLTRASATLTTTPRSMSKVEKSQEAQATATSVEVAAATAATSEKTGIQATSAAQSTAAATTAEREAIATATSSDTTATPTLAAAAAIQSAGNIPAQLTTKTLRPTETTSLAQLQRRQQMPGMPAKTTETPAKNLPSLAQTKATTALPILATRDAATATTEINSDKPTNISGATKTVATSAAEIATPPAIEVPQTILAGKKSDKMPADKAHETLLKYPTRDTSGQVATTPHKHATLQLHVKDRHLIGTPLLMHKGDVLLVDAEQLLLPGTATVADADSEVLDPSQQHQSAEQEKHQSATDKRQADAINGDTKSPAKGHKKKPSLSIKKMTYSTKHAAKTVEDMAATSKTPQHQHSSVNTPKEAAPEELSTFAQLKAYVELKSESKPEHLMDQREENHHNLTGNHPGVMLLVACVLFIVLLAGLAHVYRCELPWQRSNRSGQLRPHHQRHLNETDDAHSFLHYQGSVNSNGGDPARLQKWHHSTRREAPYSSPLHNLQARELQQQRCQQFYSSSLADKSSSTSSSSSGSSRSSLHSPSRDDSYYIEMAPSSPPAANLPSLPMELLGSRSNALGCRTDRVAATDLGGTTEAVPSSAAAIKSVSSRLMTPSSRRLNIW

Target ORF information:

RefSeq Version	NM_142001.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 2mit, transcript variant A (2mit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142001.4

ORF Insert Sequence:

ATGGTGAAAA TCGTGAACCA ACACTGTGAT CTAGGCCTGG CCCTTCTCCT GGCCTGGACG 
TGGCTAACAA GGCTGGTGGT GGCGGCCCAT CTGGTCGACA TCCCCACCTC ATCACGCCTG 
GCCGCTGAAC GGGAGGAGCA GCAGTTGTCC CGGCAGGACG TAGGACGGCT CAGCTACCAG 
AGTATCCATC GTATGCTGAG GGACGAAAAC GAACCGGATA GCTTTCGGGG GGAATTGCGA 
TACCAACAGA AACGCCACAA AAGGGAGCTG GAGCTGAATG CGCCGGCCAA CAAGCTTAAT 
CTCACCCACC GCGATTTGAG GACATTCAAT AGCACTGGTG GTCAGTGGAA GGGCGACTTT 
CAAGTGATCA CCGCCATGGA TCTGAGCAGC AATCAACTGG AGAGCCTCAG CTTGGACAAC 
TTCAATCAAC TGAGGCAGCT GGACTTGGGA AATAACTCCC TGGAGGTAAT ACCCTTGAGT 
TTGGCAGACA CCAATATGTC ACTACCCTTT GTGACGCTCG ATCTTTCCTG CAACAAATTC 
AGCCAAATTT CTACGAGCTT TTTTGCCCAG CGATTGCCTC AGTTGAAAAA TCTGAATCTG 
GCTCACAATG AATTGCTAAA TATTTCCCGG GAATCATTCT ATAATTTATT GGAACTACAA 
ACGTTAGTTC TCAGTCACAA CAACATCTCG GATATTGACT ATGAAACATT TTTGGCACTA 
CCGAATCTGC AATATCTGGA TTTATCCCAT AACCGCTTGA GTGGATCCGC TATTCGTGCT 
CTGCAAGGAA TTCCGGATTT GGTCAGCCTT TCCATCGCTT ACAATCCAGA TGTGGGAGTG 
GCGATGCAGG AGTTCGTTGC TTCCTGGAGC CTAAAGGAGT TGGATGCCAG TGGCACTGGA 
TTGTGTCAGG TGCCTGCAGC TCTAGCCCAA TCCGTGAGGA CTCTCAAGTT GTCCGACAAC 
TGGCTCAAGG CAATTAATTG CGGTGACATG GACAGCTATC CGCTGCTGCA GTATCTTGAT 
CTCTCGCACT CCCGCATTGC CCAAGTGGAG GACGATGCCT TGGGACGATT GGAGCTCCTC 
GAATCCCTTT TCCTAGACCG TAATCTACTG ATGCGAGTGC CAAGTAGTCT GCCGCCATCG 
CTGGAACACT TATTTCTGCA GCACAATCAG ATAATGGAGC TTCCGCCACA GGCTTTTGTG 
GGATTGGTCA ATCTACAGAC TCTGGACTTA TCCAACAATC GATTGATCTT CCTGCCCCCG 
CTATCGCTCC CCAAATTGCT CACCCTGAAT CTGGAATCGT CAGGGGTGGA GAGCGTTAGC 
CAATCGATAG TGCACACACT GCCACAGTTA AGGGATCTTC TACTGGAGGA CAACCCCATT 
AAGTGCAGCG ATTTGCTGGG CATTGCCGAA TGGGCCAGTC CTTGCAGGTC AGTGGATGCG 
GGTCAATCGA ATGGGGCAAG TGTAAGTGGG CGCGTGGATT TGAAGACGGA GTATCTGCAA 
TTCCACAATT TTTACGAAAA CTTCAGCAGC CGAGAGTGTG GTATAAGAAA ACCGGAAAAT 
GACACAAAGC CGCCTTCTTG CAGCCTAACA AGGGCATCAG CTACATTAAC AACAACACCC 
AGAAGTATGT CAAAAGTTGA AAAGTCACAA GAAGCACAAG CAACAGCAAC ATCAGTGGAA 
GTAGCAGCGG CAACAGCAGC AACATCAGAA AAAACAGGCA TACAAGCAAC ATCAGCAGCA 
CAGTCGACTG CGGCGGCAAC AACAGCAGAA AGAGAAGCAA TTGCAACAGC AACATCAAGC 
GACACCACAG CAACACCAAC ACTCGCAGCA GCAGCAGCAA TACAATCAGC TGGCAACATC 
CCTGCCCAGT TAACCACAAA AACGTTGCGG CCCACAGAAA CAACTTCGTT AGCGCAACTG 
CAGCGTCGGC AACAAATGCC TGGCATGCCG GCCAAAACAA CAGAAACGCC AGCAAAGAAC 
CTGCCAAGTT TGGCCCAGAC AAAGGCAACA ACAGCGCTTC CCATTTTGGC AACACGAGAT 
GCTGCCACAG CAACAACGGA AATAAATTCC GACAAGCCAA CGAACATTAG CGGTGCCACA 
AAAACAGTAG CAACATCAGC TGCAGAAATA GCAACACCAC CAGCAATTGA AGTGCCACAA 
ACCATTTTGG CCGGCAAAAA ATCTGACAAA ATGCCAGCCG ATAAGGCACA CGAGACTTTA 
TTAAAATACC CAACAAGGGA CACATCCGGT CAAGTTGCAA CAACGCCACA CAAACATGCA 
ACACTGCAGC TGCACGTTAA GGATCGACAT CTAATTGGCA CACCGCTGCT GATGCACAAG 
GGCGATGTCC TATTGGTGGA TGCCGAGCAG TTGTTGCTGC CTGGTACGGC CACCGTGGCG 
GATGCGGATT CGGAAGTTCT GGACCCGAGC CAACAACATC AGTCAGCGGA GCAGGAAAAG 
CACCAGTCAG CGACTGATAA GCGGCAAGCG GATGCAATAA ACGGCGACAC GAAGTCGCCG 
GCGAAAGGCC ACAAAAAGAA ACCATCGCTG AGCATCAAGA AGATGACCTA CAGTACCAAA 
CACGCGGCAA AAACCGTGGA GGATATGGCA GCCACCTCGA AGACACCGCA ACACCAACAT 
AGCAGTGTGA ACACACCCAA AGAGGCTGCT CCCGAGGAGT TGAGCACCTT TGCGCAGCTT 
AAAGCCTATG TGGAGCTAAA GAGCGAATCG AAACCGGAAC ACCTAATGGA CCAGCGGGAG 
GAAAACCATC ATAATCTTAC AGGAAATCAT CCAGGAGTCA TGCTCCTGGT GGCCTGCGTT 
TTGTTCATCG TCCTGCTCGC CGGTTTGGCC CACGTCTATC GCTGTGAATT GCCTTGGCAA 
AGGAGCAACC GCTCTGGTCA ATTGCGACCG CATCATCAAA GACACCTAAA CGAAACCGAT 
GATGCGCACA GCTTCCTGCA CTATCAGGGA TCTGTGAACT CCAATGGCGG TGATCCGGCT 
CGCCTGCAAA AGTGGCACCA CAGCACGCGG AGAGAAGCAC CCTACAGCTC CCCGCTGCAC 
AATCTACAAG CGCGGGAACT GCAACAGCAA CGCTGCCAGC AATTCTATAG CTCCTCGCTG 
GCGGACAAGA GCTCCTCCAC CTCGTCTTCC TCGTCGGGAA GCAGTCGCAG TAGCCTGCAC 
TCGCCCAGCA GAGATGACAG CTACTATATA GAGATGGCGC CCAGTAGTCC ACCAGCAGCC 
AACCTGCCCA GTTTGCCCAT GGAACTTTTG GGCAGCCGGA GTAATGCCCT CGGATGCCGG 
ACGGATCGAG TGGCAGCCAC CGATCTGGGC GGTACGACAG AGGCGGTACC CTCTTCGGCG 
GCGGCCATCA AGTCGGTGAG CAGCAGACTG ATGACGCCCA GTTCGCGGAG GCTGAACATC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

2mit ( NM_001300381.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287310.1 Drosophila melanogaster 2mit, transcript variant C (2mit), mRNA.

Price & Availability↑

CloneID	OFa04769
Clone ID Related Accession (Same CDS sequence)	NM_142001.4 , NM_001300381.1 , NM_001300382.1
Accession Version	NM_001300381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	2mit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAAAA TCGTGAACCA ACACTGTGAT CTAGGCCTGG CCCTTCTCCT GGCCTGGACG 
TGGCTAACAA GGCTGGTGGT GGCGGCCCAT CTGGTCGACA TCCCCACCTC ATCACGCCTG 
GCCGCTGAAC GGGAGGAGCA GCAGTTGTCC CGGCAGGACG TAGGACGGCT CAGCTACCAG 
AGTATCCATC GTATGCTGAG GGACGAAAAC GAACCGGATA GCTTTCGGGG GGAATTGCGA 
TACCAACAGA AACGCCACAA AAGGGAGCTG GAGCTGAATG CGCCGGCCAA CAAGCTTAAT 
CTCACCCACC GCGATTTGAG GACATTCAAT AGCACTGGTG GTCAGTGGAA GGGCGACTTT 
CAAGTGATCA CCGCCATGGA TCTGAGCAGC AATCAACTGG AGAGCCTCAG CTTGGACAAC 
TTCAATCAAC TGAGGCAGCT GGACTTGGGA AATAACTCCC TGGAGGTAAT ACCCTTGAGT 
TTGGCAGACA CCAATATGTC ACTACCCTTT GTGACGCTCG ATCTTTCCTG CAACAAATTC 
AGCCAAATTT CTACGAGCTT TTTTGCCCAG CGATTGCCTC AGTTGAAAAA TCTGAATCTG 
GCTCACAATG AATTGCTAAA TATTTCCCGG GAATCATTCT ATAATTTATT GGAACTACAA 
ACGTTAGTTC TCAGTCACAA CAACATCTCG GATATTGACT ATGAAACATT TTTGGCACTA 
CCGAATCTGC AATATCTGGA TTTATCCCAT AACCGCTTGA GTGGATCCGC TATTCGTGCT 
CTGCAAGGAA TTCCGGATTT GGTCAGCCTT TCCATCGCTT ACAATCCAGA TGTGGGAGTG 
GCGATGCAGG AGTTCGTTGC TTCCTGGAGC CTAAAGGAGT TGGATGCCAG TGGCACTGGA 
TTGTGTCAGG TGCCTGCAGC TCTAGCCCAA TCCGTGAGGA CTCTCAAGTT GTCCGACAAC 
TGGCTCAAGG CAATTAATTG CGGTGACATG GACAGCTATC CGCTGCTGCA GTATCTTGAT 
CTCTCGCACT CCCGCATTGC CCAAGTGGAG GACGATGCCT TGGGACGATT GGAGCTCCTC 
GAATCCCTTT TCCTAGACCG TAATCTACTG ATGCGAGTGC CAAGTAGTCT GCCGCCATCG 
CTGGAACACT TATTTCTGCA GCACAATCAG ATAATGGAGC TTCCGCCACA GGCTTTTGTG 
GGATTGGTCA ATCTACAGAC TCTGGACTTA TCCAACAATC GATTGATCTT CCTGCCCCCG 
CTATCGCTCC CCAAATTGCT CACCCTGAAT CTGGAATCGT CAGGGGTGGA GAGCGTTAGC 
CAATCGATAG TGCACACACT GCCACAGTTA AGGGATCTTC TACTGGAGGA CAACCCCATT 
AAGTGCAGCG ATTTGCTGGG CATTGCCGAA TGGGCCAGTC CTTGCAGGTC AGTGGATGCG 
GGTCAATCGA ATGGGGCAAG TGTAAGTGGG CGCGTGGATT TGAAGACGGA GTATCTGCAA 
TTCCACAATT TTTACGAAAA CTTCAGCAGC CGAGAGTGTG GTATAAGAAA ACCGGAAAAT 
GACACAAAGC CGCCTTCTTG CAGCCTAACA AGGGCATCAG CTACATTAAC AACAACACCC 
AGAAGTATGT CAAAAGTTGA AAAGTCACAA GAAGCACAAG CAACAGCAAC ATCAGTGGAA 
GTAGCAGCGG CAACAGCAGC AACATCAGAA AAAACAGGCA TACAAGCAAC ATCAGCAGCA 
CAGTCGACTG CGGCGGCAAC AACAGCAGAA AGAGAAGCAA TTGCAACAGC AACATCAAGC 
GACACCACAG CAACACCAAC ACTCGCAGCA GCAGCAGCAA TACAATCAGC TGGCAACATC 
CCTGCCCAGT TAACCACAAA AACGTTGCGG CCCACAGAAA CAACTTCGTT AGCGCAACTG 
CAGCGTCGGC AACAAATGCC TGGCATGCCG GCCAAAACAA CAGAAACGCC AGCAAAGAAC 
CTGCCAAGTT TGGCCCAGAC AAAGGCAACA ACAGCGCTTC CCATTTTGGC AACACGAGAT 
GCTGCCACAG CAACAACGGA AATAAATTCC GACAAGCCAA CGAACATTAG CGGTGCCACA 
AAAACAGTAG CAACATCAGC TGCAGAAATA GCAACACCAC CAGCAATTGA AGTGCCACAA 
ACCATTTTGG CCGGCAAAAA ATCTGACAAA ATGCCAGCCG ATAAGGCACA CGAGACTTTA 
TTAAAATACC CAACAAGGGA CACATCCGGT CAAGTTGCAA CAACGCCACA CAAACATGCA 
ACACTGCAGC TGCACGTTAA GGATCGACAT CTAATTGGCA CACCGCTGCT GATGCACAAG 
GGCGATGTCC TATTGGTGGA TGCCGAGCAG TTGTTGCTGC CTGGTACGGC CACCGTGGCG 
GATGCGGATT CGGAAGTTCT GGACCCGAGC CAACAACATC AGTCAGCGGA GCAGGAAAAG 
CACCAGTCAG CGACTGATAA GCGGCAAGCG GATGCAATAA ACGGCGACAC GAAGTCGCCG 
GCGAAAGGCC ACAAAAAGAA ACCATCGCTG AGCATCAAGA AGATGACCTA CAGTACCAAA 
CACGCGGCAA AAACCGTGGA GGATATGGCA GCCACCTCGA AGACACCGCA ACACCAACAT 
AGCAGTGTGA ACACACCCAA AGAGGCTGCT CCCGAGGAGT TGAGCACCTT TGCGCAGCTT 
AAAGCCTATG TGGAGCTAAA GAGCGAATCG AAACCGGAAC ACCTAATGGA CCAGCGGGAG 
GAAAACCATC ATAATCTTAC AGGAAATCAT CCAGGAGTCA TGCTCCTGGT GGCCTGCGTT 
TTGTTCATCG TCCTGCTCGC CGGTTTGGCC CACGTCTATC GCTGTGAATT GCCTTGGCAA 
AGGAGCAACC GCTCTGGTCA ATTGCGACCG CATCATCAAA GACACCTAAA CGAAACCGAT 
GATGCGCACA GCTTCCTGCA CTATCAGGGA TCTGTGAACT CCAATGGCGG TGATCCGGCT 
CGCCTGCAAA AGTGGCACCA CAGCACGCGG AGAGAAGCAC CCTACAGCTC CCCGCTGCAC 
AATCTACAAG CGCGGGAACT GCAACAGCAA CGCTGCCAGC AATTCTATAG CTCCTCGCTG 
GCGGACAAGA GCTCCTCCAC CTCGTCTTCC TCGTCGGGAA GCAGTCGCAG TAGCCTGCAC 
TCGCCCAGCA GAGATGACAG CTACTATATA GAGATGGCGC CCAGTAGTCC ACCAGCAGCC 
AACCTGCCCA GTTTGCCCAT GGAACTTTTG GGCAGCCGGA GTAATGCCCT CGGATGCCGG 
ACGGATCGAG TGGCAGCCAC CGATCTGGGC GGTACGACAG AGGCGGTACC CTCTTCGGCG 
GCGGCCATCA AGTCGGTGAG CAGCAGACTG ATGACGCCCA GTTCGCGGAG GCTGAACATC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287310.1
CDS	82..3507
Translation	MVKIVNQHCDLGLALLLAWTWLTRLVVAAHLVDIPTSSRLAAEREEQQLSRQDVGRLSYQSIHRMLRDENEPDSFRGELRYQQKRHKRELELNAPANKLNLTHRDLRTFNSTGGQWKGDFQVITAMDLSSNQLESLSLDNFNQLRQLDLGNNSLEVIPLSLADTNMSLPFVTLDLSCNKFSQISTSFFAQRLPQLKNLNLAHNELLNISRESFYNLLELQTLVLSHNNISDIDYETFLALPNLQYLDLSHNRLSGSAIRALQGIPDLVSLSIAYNPDVGVAMQEFVASWSLKELDASGTGLCQVPAALAQSVRTLKLSDNWLKAINCGDMDSYPLLQYLDLSHSRIAQVEDDALGRLELLESLFLDRNLLMRVPSSLPPSLEHLFLQHNQIMELPPQAFVGLVNLQTLDLSNNRLIFLPPLSLPKLLTLNLESSGVESVSQSIVHTLPQLRDLLLEDNPIKCSDLLGIAEWASPCRSVDAGQSNGASVSGRVDLKTEYLQFHNFYENFSSRECGIRKPENDTKPPSCSLTRASATLTTTPRSMSKVEKSQEAQATATSVEVAAATAATSEKTGIQATSAAQSTAAATTAEREAIATATSSDTTATPTLAAAAAIQSAGNIPAQLTTKTLRPTETTSLAQLQRRQQMPGMPAKTTETPAKNLPSLAQTKATTALPILATRDAATATTEINSDKPTNISGATKTVATSAAEIATPPAIEVPQTILAGKKSDKMPADKAHETLLKYPTRDTSGQVATTPHKHATLQLHVKDRHLIGTPLLMHKGDVLLVDAEQLLLPGTATVADADSEVLDPSQQHQSAEQEKHQSATDKRQADAINGDTKSPAKGHKKKPSLSIKKMTYSTKHAAKTVEDMAATSKTPQHQHSSVNTPKEAAPEELSTFAQLKAYVELKSESKPEHLMDQREENHHNLTGNHPGVMLLVACVLFIVLLAGLAHVYRCELPWQRSNRSGQLRPHHQRHLNETDDAHSFLHYQGSVNSNGGDPARLQKWHHSTRREAPYSSPLHNLQARELQQQRCQQFYSSSLADKSSSTSSSSSGSSRSSLHSPSRDDSYYIEMAPSSPPAANLPSLPMELLGSRSNALGCRTDRVAATDLGGTTEAVPSSAAAIKSVSSRLMTPSSRRLNIW

Target ORF information:

RefSeq Version	NM_001300381.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 2mit, transcript variant C (2mit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300381.1

ORF Insert Sequence:

ATGGTGAAAA TCGTGAACCA ACACTGTGAT CTAGGCCTGG CCCTTCTCCT GGCCTGGACG 
TGGCTAACAA GGCTGGTGGT GGCGGCCCAT CTGGTCGACA TCCCCACCTC ATCACGCCTG 
GCCGCTGAAC GGGAGGAGCA GCAGTTGTCC CGGCAGGACG TAGGACGGCT CAGCTACCAG 
AGTATCCATC GTATGCTGAG GGACGAAAAC GAACCGGATA GCTTTCGGGG GGAATTGCGA 
TACCAACAGA AACGCCACAA AAGGGAGCTG GAGCTGAATG CGCCGGCCAA CAAGCTTAAT 
CTCACCCACC GCGATTTGAG GACATTCAAT AGCACTGGTG GTCAGTGGAA GGGCGACTTT 
CAAGTGATCA CCGCCATGGA TCTGAGCAGC AATCAACTGG AGAGCCTCAG CTTGGACAAC 
TTCAATCAAC TGAGGCAGCT GGACTTGGGA AATAACTCCC TGGAGGTAAT ACCCTTGAGT 
TTGGCAGACA CCAATATGTC ACTACCCTTT GTGACGCTCG ATCTTTCCTG CAACAAATTC 
AGCCAAATTT CTACGAGCTT TTTTGCCCAG CGATTGCCTC AGTTGAAAAA TCTGAATCTG 
GCTCACAATG AATTGCTAAA TATTTCCCGG GAATCATTCT ATAATTTATT GGAACTACAA 
ACGTTAGTTC TCAGTCACAA CAACATCTCG GATATTGACT ATGAAACATT TTTGGCACTA 
CCGAATCTGC AATATCTGGA TTTATCCCAT AACCGCTTGA GTGGATCCGC TATTCGTGCT 
CTGCAAGGAA TTCCGGATTT GGTCAGCCTT TCCATCGCTT ACAATCCAGA TGTGGGAGTG 
GCGATGCAGG AGTTCGTTGC TTCCTGGAGC CTAAAGGAGT TGGATGCCAG TGGCACTGGA 
TTGTGTCAGG TGCCTGCAGC TCTAGCCCAA TCCGTGAGGA CTCTCAAGTT GTCCGACAAC 
TGGCTCAAGG CAATTAATTG CGGTGACATG GACAGCTATC CGCTGCTGCA GTATCTTGAT 
CTCTCGCACT CCCGCATTGC CCAAGTGGAG GACGATGCCT TGGGACGATT GGAGCTCCTC 
GAATCCCTTT TCCTAGACCG TAATCTACTG ATGCGAGTGC CAAGTAGTCT GCCGCCATCG 
CTGGAACACT TATTTCTGCA GCACAATCAG ATAATGGAGC TTCCGCCACA GGCTTTTGTG 
GGATTGGTCA ATCTACAGAC TCTGGACTTA TCCAACAATC GATTGATCTT CCTGCCCCCG 
CTATCGCTCC CCAAATTGCT CACCCTGAAT CTGGAATCGT CAGGGGTGGA GAGCGTTAGC 
CAATCGATAG TGCACACACT GCCACAGTTA AGGGATCTTC TACTGGAGGA CAACCCCATT 
AAGTGCAGCG ATTTGCTGGG CATTGCCGAA TGGGCCAGTC CTTGCAGGTC AGTGGATGCG 
GGTCAATCGA ATGGGGCAAG TGTAAGTGGG CGCGTGGATT TGAAGACGGA GTATCTGCAA 
TTCCACAATT TTTACGAAAA CTTCAGCAGC CGAGAGTGTG GTATAAGAAA ACCGGAAAAT 
GACACAAAGC CGCCTTCTTG CAGCCTAACA AGGGCATCAG CTACATTAAC AACAACACCC 
AGAAGTATGT CAAAAGTTGA AAAGTCACAA GAAGCACAAG CAACAGCAAC ATCAGTGGAA 
GTAGCAGCGG CAACAGCAGC AACATCAGAA AAAACAGGCA TACAAGCAAC ATCAGCAGCA 
CAGTCGACTG CGGCGGCAAC AACAGCAGAA AGAGAAGCAA TTGCAACAGC AACATCAAGC 
GACACCACAG CAACACCAAC ACTCGCAGCA GCAGCAGCAA TACAATCAGC TGGCAACATC 
CCTGCCCAGT TAACCACAAA AACGTTGCGG CCCACAGAAA CAACTTCGTT AGCGCAACTG 
CAGCGTCGGC AACAAATGCC TGGCATGCCG GCCAAAACAA CAGAAACGCC AGCAAAGAAC 
CTGCCAAGTT TGGCCCAGAC AAAGGCAACA ACAGCGCTTC CCATTTTGGC AACACGAGAT 
GCTGCCACAG CAACAACGGA AATAAATTCC GACAAGCCAA CGAACATTAG CGGTGCCACA 
AAAACAGTAG CAACATCAGC TGCAGAAATA GCAACACCAC CAGCAATTGA AGTGCCACAA 
ACCATTTTGG CCGGCAAAAA ATCTGACAAA ATGCCAGCCG ATAAGGCACA CGAGACTTTA 
TTAAAATACC CAACAAGGGA CACATCCGGT CAAGTTGCAA CAACGCCACA CAAACATGCA 
ACACTGCAGC TGCACGTTAA GGATCGACAT CTAATTGGCA CACCGCTGCT GATGCACAAG 
GGCGATGTCC TATTGGTGGA TGCCGAGCAG TTGTTGCTGC CTGGTACGGC CACCGTGGCG 
GATGCGGATT CGGAAGTTCT GGACCCGAGC CAACAACATC AGTCAGCGGA GCAGGAAAAG 
CACCAGTCAG CGACTGATAA GCGGCAAGCG GATGCAATAA ACGGCGACAC GAAGTCGCCG 
GCGAAAGGCC ACAAAAAGAA ACCATCGCTG AGCATCAAGA AGATGACCTA CAGTACCAAA 
CACGCGGCAA AAACCGTGGA GGATATGGCA GCCACCTCGA AGACACCGCA ACACCAACAT 
AGCAGTGTGA ACACACCCAA AGAGGCTGCT CCCGAGGAGT TGAGCACCTT TGCGCAGCTT 
AAAGCCTATG TGGAGCTAAA GAGCGAATCG AAACCGGAAC ACCTAATGGA CCAGCGGGAG 
GAAAACCATC ATAATCTTAC AGGAAATCAT CCAGGAGTCA TGCTCCTGGT GGCCTGCGTT 
TTGTTCATCG TCCTGCTCGC CGGTTTGGCC CACGTCTATC GCTGTGAATT GCCTTGGCAA 
AGGAGCAACC GCTCTGGTCA ATTGCGACCG CATCATCAAA GACACCTAAA CGAAACCGAT 
GATGCGCACA GCTTCCTGCA CTATCAGGGA TCTGTGAACT CCAATGGCGG TGATCCGGCT 
CGCCTGCAAA AGTGGCACCA CAGCACGCGG AGAGAAGCAC CCTACAGCTC CCCGCTGCAC 
AATCTACAAG CGCGGGAACT GCAACAGCAA CGCTGCCAGC AATTCTATAG CTCCTCGCTG 
GCGGACAAGA GCTCCTCCAC CTCGTCTTCC TCGTCGGGAA GCAGTCGCAG TAGCCTGCAC 
TCGCCCAGCA GAGATGACAG CTACTATATA GAGATGGCGC CCAGTAGTCC ACCAGCAGCC 
AACCTGCCCA GTTTGCCCAT GGAACTTTTG GGCAGCCGGA GTAATGCCCT CGGATGCCGG 
ACGGATCGAG TGGCAGCCAC CGATCTGGGC GGTACGACAG AGGCGGTACC CTCTTCGGCG 
GCGGCCATCA AGTCGGTGAG CAGCAGACTG ATGACGCCCA GTTCGCGGAG GCTGAACATC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

2mit ( NM_001300382.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287311.1 Drosophila melanogaster 2mit, transcript variant D (2mit), mRNA.

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CloneID	OFa04769
Clone ID Related Accession (Same CDS sequence)	NM_142001.4 , NM_001300381.1 , NM_001300382.1
Accession Version	NM_001300382.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	2mit, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAAAA TCGTGAACCA ACACTGTGAT CTAGGCCTGG CCCTTCTCCT GGCCTGGACG 
TGGCTAACAA GGCTGGTGGT GGCGGCCCAT CTGGTCGACA TCCCCACCTC ATCACGCCTG 
GCCGCTGAAC GGGAGGAGCA GCAGTTGTCC CGGCAGGACG TAGGACGGCT CAGCTACCAG 
AGTATCCATC GTATGCTGAG GGACGAAAAC GAACCGGATA GCTTTCGGGG GGAATTGCGA 
TACCAACAGA AACGCCACAA AAGGGAGCTG GAGCTGAATG CGCCGGCCAA CAAGCTTAAT 
CTCACCCACC GCGATTTGAG GACATTCAAT AGCACTGGTG GTCAGTGGAA GGGCGACTTT 
CAAGTGATCA CCGCCATGGA TCTGAGCAGC AATCAACTGG AGAGCCTCAG CTTGGACAAC 
TTCAATCAAC TGAGGCAGCT GGACTTGGGA AATAACTCCC TGGAGGTAAT ACCCTTGAGT 
TTGGCAGACA CCAATATGTC ACTACCCTTT GTGACGCTCG ATCTTTCCTG CAACAAATTC 
AGCCAAATTT CTACGAGCTT TTTTGCCCAG CGATTGCCTC AGTTGAAAAA TCTGAATCTG 
GCTCACAATG AATTGCTAAA TATTTCCCGG GAATCATTCT ATAATTTATT GGAACTACAA 
ACGTTAGTTC TCAGTCACAA CAACATCTCG GATATTGACT ATGAAACATT TTTGGCACTA 
CCGAATCTGC AATATCTGGA TTTATCCCAT AACCGCTTGA GTGGATCCGC TATTCGTGCT 
CTGCAAGGAA TTCCGGATTT GGTCAGCCTT TCCATCGCTT ACAATCCAGA TGTGGGAGTG 
GCGATGCAGG AGTTCGTTGC TTCCTGGAGC CTAAAGGAGT TGGATGCCAG TGGCACTGGA 
TTGTGTCAGG TGCCTGCAGC TCTAGCCCAA TCCGTGAGGA CTCTCAAGTT GTCCGACAAC 
TGGCTCAAGG CAATTAATTG CGGTGACATG GACAGCTATC CGCTGCTGCA GTATCTTGAT 
CTCTCGCACT CCCGCATTGC CCAAGTGGAG GACGATGCCT TGGGACGATT GGAGCTCCTC 
GAATCCCTTT TCCTAGACCG TAATCTACTG ATGCGAGTGC CAAGTAGTCT GCCGCCATCG 
CTGGAACACT TATTTCTGCA GCACAATCAG ATAATGGAGC TTCCGCCACA GGCTTTTGTG 
GGATTGGTCA ATCTACAGAC TCTGGACTTA TCCAACAATC GATTGATCTT CCTGCCCCCG 
CTATCGCTCC CCAAATTGCT CACCCTGAAT CTGGAATCGT CAGGGGTGGA GAGCGTTAGC 
CAATCGATAG TGCACACACT GCCACAGTTA AGGGATCTTC TACTGGAGGA CAACCCCATT 
AAGTGCAGCG ATTTGCTGGG CATTGCCGAA TGGGCCAGTC CTTGCAGGTC AGTGGATGCG 
GGTCAATCGA ATGGGGCAAG TGTAAGTGGG CGCGTGGATT TGAAGACGGA GTATCTGCAA 
TTCCACAATT TTTACGAAAA CTTCAGCAGC CGAGAGTGTG GTATAAGAAA ACCGGAAAAT 
GACACAAAGC CGCCTTCTTG CAGCCTAACA AGGGCATCAG CTACATTAAC AACAACACCC 
AGAAGTATGT CAAAAGTTGA AAAGTCACAA GAAGCACAAG CAACAGCAAC ATCAGTGGAA 
GTAGCAGCGG CAACAGCAGC AACATCAGAA AAAACAGGCA TACAAGCAAC ATCAGCAGCA 
CAGTCGACTG CGGCGGCAAC AACAGCAGAA AGAGAAGCAA TTGCAACAGC AACATCAAGC 
GACACCACAG CAACACCAAC ACTCGCAGCA GCAGCAGCAA TACAATCAGC TGGCAACATC 
CCTGCCCAGT TAACCACAAA AACGTTGCGG CCCACAGAAA CAACTTCGTT AGCGCAACTG 
CAGCGTCGGC AACAAATGCC TGGCATGCCG GCCAAAACAA CAGAAACGCC AGCAAAGAAC 
CTGCCAAGTT TGGCCCAGAC AAAGGCAACA ACAGCGCTTC CCATTTTGGC AACACGAGAT 
GCTGCCACAG CAACAACGGA AATAAATTCC GACAAGCCAA CGAACATTAG CGGTGCCACA 
AAAACAGTAG CAACATCAGC TGCAGAAATA GCAACACCAC CAGCAATTGA AGTGCCACAA 
ACCATTTTGG CCGGCAAAAA ATCTGACAAA ATGCCAGCCG ATAAGGCACA CGAGACTTTA 
TTAAAATACC CAACAAGGGA CACATCCGGT CAAGTTGCAA CAACGCCACA CAAACATGCA 
ACACTGCAGC TGCACGTTAA GGATCGACAT CTAATTGGCA CACCGCTGCT GATGCACAAG 
GGCGATGTCC TATTGGTGGA TGCCGAGCAG TTGTTGCTGC CTGGTACGGC CACCGTGGCG 
GATGCGGATT CGGAAGTTCT GGACCCGAGC CAACAACATC AGTCAGCGGA GCAGGAAAAG 
CACCAGTCAG CGACTGATAA GCGGCAAGCG GATGCAATAA ACGGCGACAC GAAGTCGCCG 
GCGAAAGGCC ACAAAAAGAA ACCATCGCTG AGCATCAAGA AGATGACCTA CAGTACCAAA 
CACGCGGCAA AAACCGTGGA GGATATGGCA GCCACCTCGA AGACACCGCA ACACCAACAT 
AGCAGTGTGA ACACACCCAA AGAGGCTGCT CCCGAGGAGT TGAGCACCTT TGCGCAGCTT 
AAAGCCTATG TGGAGCTAAA GAGCGAATCG AAACCGGAAC ACCTAATGGA CCAGCGGGAG 
GAAAACCATC ATAATCTTAC AGGAAATCAT CCAGGAGTCA TGCTCCTGGT GGCCTGCGTT 
TTGTTCATCG TCCTGCTCGC CGGTTTGGCC CACGTCTATC GCTGTGAATT GCCTTGGCAA 
AGGAGCAACC GCTCTGGTCA ATTGCGACCG CATCATCAAA GACACCTAAA CGAAACCGAT 
GATGCGCACA GCTTCCTGCA CTATCAGGGA TCTGTGAACT CCAATGGCGG TGATCCGGCT 
CGCCTGCAAA AGTGGCACCA CAGCACGCGG AGAGAAGCAC CCTACAGCTC CCCGCTGCAC 
AATCTACAAG CGCGGGAACT GCAACAGCAA CGCTGCCAGC AATTCTATAG CTCCTCGCTG 
GCGGACAAGA GCTCCTCCAC CTCGTCTTCC TCGTCGGGAA GCAGTCGCAG TAGCCTGCAC 
TCGCCCAGCA GAGATGACAG CTACTATATA GAGATGGCGC CCAGTAGTCC ACCAGCAGCC 
AACCTGCCCA GTTTGCCCAT GGAACTTTTG GGCAGCCGGA GTAATGCCCT CGGATGCCGG 
ACGGATCGAG TGGCAGCCAC CGATCTGGGC GGTACGACAG AGGCGGTACC CTCTTCGGCG 
GCGGCCATCA AGTCGGTGAG CAGCAGACTG ATGACGCCCA GTTCGCGGAG GCTGAACATC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287311.1
CDS	82..3507
Translation	MVKIVNQHCDLGLALLLAWTWLTRLVVAAHLVDIPTSSRLAAEREEQQLSRQDVGRLSYQSIHRMLRDENEPDSFRGELRYQQKRHKRELELNAPANKLNLTHRDLRTFNSTGGQWKGDFQVITAMDLSSNQLESLSLDNFNQLRQLDLGNNSLEVIPLSLADTNMSLPFVTLDLSCNKFSQISTSFFAQRLPQLKNLNLAHNELLNISRESFYNLLELQTLVLSHNNISDIDYETFLALPNLQYLDLSHNRLSGSAIRALQGIPDLVSLSIAYNPDVGVAMQEFVASWSLKELDASGTGLCQVPAALAQSVRTLKLSDNWLKAINCGDMDSYPLLQYLDLSHSRIAQVEDDALGRLELLESLFLDRNLLMRVPSSLPPSLEHLFLQHNQIMELPPQAFVGLVNLQTLDLSNNRLIFLPPLSLPKLLTLNLESSGVESVSQSIVHTLPQLRDLLLEDNPIKCSDLLGIAEWASPCRSVDAGQSNGASVSGRVDLKTEYLQFHNFYENFSSRECGIRKPENDTKPPSCSLTRASATLTTTPRSMSKVEKSQEAQATATSVEVAAATAATSEKTGIQATSAAQSTAAATTAEREAIATATSSDTTATPTLAAAAAIQSAGNIPAQLTTKTLRPTETTSLAQLQRRQQMPGMPAKTTETPAKNLPSLAQTKATTALPILATRDAATATTEINSDKPTNISGATKTVATSAAEIATPPAIEVPQTILAGKKSDKMPADKAHETLLKYPTRDTSGQVATTPHKHATLQLHVKDRHLIGTPLLMHKGDVLLVDAEQLLLPGTATVADADSEVLDPSQQHQSAEQEKHQSATDKRQADAINGDTKSPAKGHKKKPSLSIKKMTYSTKHAAKTVEDMAATSKTPQHQHSSVNTPKEAAPEELSTFAQLKAYVELKSESKPEHLMDQREENHHNLTGNHPGVMLLVACVLFIVLLAGLAHVYRCELPWQRSNRSGQLRPHHQRHLNETDDAHSFLHYQGSVNSNGGDPARLQKWHHSTRREAPYSSPLHNLQARELQQQRCQQFYSSSLADKSSSTSSSSSGSSRSSLHSPSRDDSYYIEMAPSSPPAANLPSLPMELLGSRSNALGCRTDRVAATDLGGTTEAVPSSAAAIKSVSSRLMTPSSRRLNIW

Target ORF information:

RefSeq Version	NM_001300382.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 2mit, transcript variant D (2mit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300382.1

ORF Insert Sequence:

ATGGTGAAAA TCGTGAACCA ACACTGTGAT CTAGGCCTGG CCCTTCTCCT GGCCTGGACG 
TGGCTAACAA GGCTGGTGGT GGCGGCCCAT CTGGTCGACA TCCCCACCTC ATCACGCCTG 
GCCGCTGAAC GGGAGGAGCA GCAGTTGTCC CGGCAGGACG TAGGACGGCT CAGCTACCAG 
AGTATCCATC GTATGCTGAG GGACGAAAAC GAACCGGATA GCTTTCGGGG GGAATTGCGA 
TACCAACAGA AACGCCACAA AAGGGAGCTG GAGCTGAATG CGCCGGCCAA CAAGCTTAAT 
CTCACCCACC GCGATTTGAG GACATTCAAT AGCACTGGTG GTCAGTGGAA GGGCGACTTT 
CAAGTGATCA CCGCCATGGA TCTGAGCAGC AATCAACTGG AGAGCCTCAG CTTGGACAAC 
TTCAATCAAC TGAGGCAGCT GGACTTGGGA AATAACTCCC TGGAGGTAAT ACCCTTGAGT 
TTGGCAGACA CCAATATGTC ACTACCCTTT GTGACGCTCG ATCTTTCCTG CAACAAATTC 
AGCCAAATTT CTACGAGCTT TTTTGCCCAG CGATTGCCTC AGTTGAAAAA TCTGAATCTG 
GCTCACAATG AATTGCTAAA TATTTCCCGG GAATCATTCT ATAATTTATT GGAACTACAA 
ACGTTAGTTC TCAGTCACAA CAACATCTCG GATATTGACT ATGAAACATT TTTGGCACTA 
CCGAATCTGC AATATCTGGA TTTATCCCAT AACCGCTTGA GTGGATCCGC TATTCGTGCT 
CTGCAAGGAA TTCCGGATTT GGTCAGCCTT TCCATCGCTT ACAATCCAGA TGTGGGAGTG 
GCGATGCAGG AGTTCGTTGC TTCCTGGAGC CTAAAGGAGT TGGATGCCAG TGGCACTGGA 
TTGTGTCAGG TGCCTGCAGC TCTAGCCCAA TCCGTGAGGA CTCTCAAGTT GTCCGACAAC 
TGGCTCAAGG CAATTAATTG CGGTGACATG GACAGCTATC CGCTGCTGCA GTATCTTGAT 
CTCTCGCACT CCCGCATTGC CCAAGTGGAG GACGATGCCT TGGGACGATT GGAGCTCCTC 
GAATCCCTTT TCCTAGACCG TAATCTACTG ATGCGAGTGC CAAGTAGTCT GCCGCCATCG 
CTGGAACACT TATTTCTGCA GCACAATCAG ATAATGGAGC TTCCGCCACA GGCTTTTGTG 
GGATTGGTCA ATCTACAGAC TCTGGACTTA TCCAACAATC GATTGATCTT CCTGCCCCCG 
CTATCGCTCC CCAAATTGCT CACCCTGAAT CTGGAATCGT CAGGGGTGGA GAGCGTTAGC 
CAATCGATAG TGCACACACT GCCACAGTTA AGGGATCTTC TACTGGAGGA CAACCCCATT 
AAGTGCAGCG ATTTGCTGGG CATTGCCGAA TGGGCCAGTC CTTGCAGGTC AGTGGATGCG 
GGTCAATCGA ATGGGGCAAG TGTAAGTGGG CGCGTGGATT TGAAGACGGA GTATCTGCAA 
TTCCACAATT TTTACGAAAA CTTCAGCAGC CGAGAGTGTG GTATAAGAAA ACCGGAAAAT 
GACACAAAGC CGCCTTCTTG CAGCCTAACA AGGGCATCAG CTACATTAAC AACAACACCC 
AGAAGTATGT CAAAAGTTGA AAAGTCACAA GAAGCACAAG CAACAGCAAC ATCAGTGGAA 
GTAGCAGCGG CAACAGCAGC AACATCAGAA AAAACAGGCA TACAAGCAAC ATCAGCAGCA 
CAGTCGACTG CGGCGGCAAC AACAGCAGAA AGAGAAGCAA TTGCAACAGC AACATCAAGC 
GACACCACAG CAACACCAAC ACTCGCAGCA GCAGCAGCAA TACAATCAGC TGGCAACATC 
CCTGCCCAGT TAACCACAAA AACGTTGCGG CCCACAGAAA CAACTTCGTT AGCGCAACTG 
CAGCGTCGGC AACAAATGCC TGGCATGCCG GCCAAAACAA CAGAAACGCC AGCAAAGAAC 
CTGCCAAGTT TGGCCCAGAC AAAGGCAACA ACAGCGCTTC CCATTTTGGC AACACGAGAT 
GCTGCCACAG CAACAACGGA AATAAATTCC GACAAGCCAA CGAACATTAG CGGTGCCACA 
AAAACAGTAG CAACATCAGC TGCAGAAATA GCAACACCAC CAGCAATTGA AGTGCCACAA 
ACCATTTTGG CCGGCAAAAA ATCTGACAAA ATGCCAGCCG ATAAGGCACA CGAGACTTTA 
TTAAAATACC CAACAAGGGA CACATCCGGT CAAGTTGCAA CAACGCCACA CAAACATGCA 
ACACTGCAGC TGCACGTTAA GGATCGACAT CTAATTGGCA CACCGCTGCT GATGCACAAG 
GGCGATGTCC TATTGGTGGA TGCCGAGCAG TTGTTGCTGC CTGGTACGGC CACCGTGGCG 
GATGCGGATT CGGAAGTTCT GGACCCGAGC CAACAACATC AGTCAGCGGA GCAGGAAAAG 
CACCAGTCAG CGACTGATAA GCGGCAAGCG GATGCAATAA ACGGCGACAC GAAGTCGCCG 
GCGAAAGGCC ACAAAAAGAA ACCATCGCTG AGCATCAAGA AGATGACCTA CAGTACCAAA 
CACGCGGCAA AAACCGTGGA GGATATGGCA GCCACCTCGA AGACACCGCA ACACCAACAT 
AGCAGTGTGA ACACACCCAA AGAGGCTGCT CCCGAGGAGT TGAGCACCTT TGCGCAGCTT 
AAAGCCTATG TGGAGCTAAA GAGCGAATCG AAACCGGAAC ACCTAATGGA CCAGCGGGAG 
GAAAACCATC ATAATCTTAC AGGAAATCAT CCAGGAGTCA TGCTCCTGGT GGCCTGCGTT 
TTGTTCATCG TCCTGCTCGC CGGTTTGGCC CACGTCTATC GCTGTGAATT GCCTTGGCAA 
AGGAGCAACC GCTCTGGTCA ATTGCGACCG CATCATCAAA GACACCTAAA CGAAACCGAT 
GATGCGCACA GCTTCCTGCA CTATCAGGGA TCTGTGAACT CCAATGGCGG TGATCCGGCT 
CGCCTGCAAA AGTGGCACCA CAGCACGCGG AGAGAAGCAC CCTACAGCTC CCCGCTGCAC 
AATCTACAAG CGCGGGAACT GCAACAGCAA CGCTGCCAGC AATTCTATAG CTCCTCGCTG 
GCGGACAAGA GCTCCTCCAC CTCGTCTTCC TCGTCGGGAA GCAGTCGCAG TAGCCTGCAC 
TCGCCCAGCA GAGATGACAG CTACTATATA GAGATGGCGC CCAGTAGTCC ACCAGCAGCC 
AACCTGCCCA GTTTGCCCAT GGAACTTTTG GGCAGCCGGA GTAATGCCCT CGGATGCCGG 
ACGGATCGAG TGGCAGCCAC CGATCTGGGC GGTACGACAG AGGCGGTACC CTCTTCGGCG 
GCGGCCATCA AGTCGGTGAG CAGCAGACTG ATGACGCCCA GTTCGCGGAG GCTGAACATC 
TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

2mit ( NM_001275600.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262529.1 Drosophila melanogaster 2mit, transcript variant B (2mit), mRNA.

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CloneID	OFa07720
Clone ID Related Accession (Same CDS sequence)	NM_001275600.2
Accession Version	NM_001275600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	2mit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275600.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAAAA TCGTGAACCA ACACTGTGAT CTAGGCCTGG CCCTTCTCCT GGCCTGGACG 
TGGCTAACAA GGCTGGTGGT GGCGGCCCAT CTGGTCGACA TCCCCACCTC ATCACGCCTG 
GCCGCTGAAC GGGAGGAGCA GCAGTTGTCC CGGCAGGACG TAGGACGGCT CAGCTACCAG 
AGTATCCATC GTATGCTGAG GGACGAAAAC GAACCGGATA GCTTTCGGGG GGAATTGCGA 
TACCAACAGA AACGCCACAA AAGGGAGCTG GAGCTGAATG CGCCGGCCAA CAAGCTTAAT 
CTCACCCACC GCGATTTGAG GACATTCAAT AGCACTGGTG GTCAGTGGAA GGGCGACTTT 
CAAGTGATCA CCGCCATGGA TCTGAGCAGC AATCAACTGG AGAGCCTCAG CTTGGACAAC 
TTCAATCAAC TGAGGCAGCT GGACTTGGGA AATAACTCCC TGGAGGTAAT ACCCTTGAGT 
TTGGCAGACA CCAATATGTC ACTACCCTTT GTGACGCTCG ATCTTTCCTG CAACAAATTC 
AGCCAAATTT CTACGAGCTT TTTTGCCCAG CGATTGCCTC AGTTGAAAAA TCTGAATCTG 
GCTCACAATG AATTGCTAAA TATTTCCCGG GAATCATTCT ATAATTTATT GGAACTACAA 
ACGTTAGTTC TCAGTCACAA CAACATCTCG GATATTGACT ATGAAACATT TTTGGCACTA 
CCGAATCTGC AATATCTGGA TTTATCCCAT AACCGCTTGA GTGGATCCGC TATTCGTGCT 
CTGCAAGGAA TTCCGGATTT GGTCAGCCTT TCCATCGCTT ACAATCCAGA TGTGGGAGTG 
GCGATGCAGG AGTTCGTTGC TTCCTGGAGC CTAAAGGAGT TGGATGCCAG TGGCACTGGA 
TTGTGTCAGG TGCCTGCAGC TCTAGCCCAA TCCGTGAGGA CTCTCAAGTT GTCCGACAAC 
TGGCTCAAGG CAATTAATTG CGGTGACATG GACAGCTATC CGCTGCTGCA GTATCTTGAT 
CTCTCGCACT CCCGCATTGC CCAAGTGGAG GACGATGCCT TGGGACGATT GGAGCTCCTC 
GAATCCCTTT TCCTAGACCG TAATCTACTG ATGCGAGTGC CAAGTAGTCT GCCGCCATCG 
CTGGAACACT TATTTCTGCA GCACAATCAG ATAATGGAGC TTCCGCCACA GGCTTTTGTG 
GGATTGGTCA ATCTACAGAC TCTGGACTTA TCCAACAATC GATTGATCTT CCTGCCCCCG 
CTATCGCTCC CCAAATTGCT CACCCTGAAT CTGGAATCGT CAGGGGTGGA GAGCGTTAGC 
CAATCGATAG TGCACACACT GCCACAGTTA AGGGATCTTC TACTGGAGGA CAACCCCATT 
AAGTGCAGCG ATTTGCTGGG CATTGCCGAA TGGGCCAGTC CTTGCAGGTC AGTGGATGCG 
GGTCAATCGA ATGGGGCAAG TGTAAGTGGG CGCGTGGATT TGAAGACGGA GTATCTGCAA 
TTCCACAATT TTTACGAAAA CTTCAGCAGC CGAGAGTGTG GTATAAGAAA ACCGGAAAAT 
GACACAAAGC CGCCTTCTTG CAGCCTAACA AGGGCATCAG CTACATTAAC AACAACACCC 
AGAAGTATGT CAAAAGTTGA AAAGTCACAA GAAGCACAAG CAACAGCAAC ATCAGTGGAA 
GTAGCAGCGG CAACAGCAGC AACATCAGAA AAAACAGGCA TACAAGCAAC ATCAGCAGCA 
CAGTCGACTG CGGCGGCAAC AACAGCAGAA AGAGAAGCAA TTGCAACAGC AACATCAAGC 
GACACCACAG CAACACCAAC ACTCGCAGCA GCAGCAGCAA TACAATCAGC TGGCAACATC 
CCTGCCCAGT TAACCACAAA AACGTTGCGG CCCACAGAAA CAACTTCGTT AGCGCAACTG 
CAGCGTCGGC AACAAATGCC TGGCATGCCG GCCAAAACAA CAGAAACGCC AGCAAAGAAC 
CTGCCAAGTT TGGCCCAGAC AAAGGCAACA ACAGCGCTTC CCATTTTGGC AACACGAGAT 
GCTGCCACAG CAACAACGGA AATAAATTCC GACAAGCCAA CGAACATTAG CGGTGCCACA 
AAAACAGTAG CAACATCAGC TGCAGAAATA GCAACACCAC CAGCAATTGA AGTGCCACAA 
ACCATTTTGG CCGGCAAAAA ATCTGACAAA ATGCCAGCCG ATAAGGCACA CGAGACTTTA 
TTAAAATACC CAACAAGGGA CACATCCGGT CAAGTTGCAA CAACGCCACA CAAACATGCA 
ACACTGCAGC TGCACGTTAA GGATCGACAT CTAATTGGCA CACCGCTGCT GATGCACAAG 
GGCGATGTCC TATTGGTGGA TGCCGAGCAG TTGTTGCTGC CTGGTACGGC CACCGTGGCG 
GATGCGGATT CGGAAGTTCT GGACCCGAGC CAACAACATC AGTCAGCGGA GCAGGAAAAG 
CACCAGTCAG CGACTGATAA GCGGCAAGCG GATGCAATAA ACGGCGACAC GAAGTCGCCG 
GCGAAAGGCC ACAAAAAGAA ACCATCGCTG AGCATCAAGA AGATGACCTA CAGTACCAAA 
CACGCGGCAA AAACCGTGGA GGATATGGCA GCCACCTCGA AGACACCGCA ACACCAACAT 
AGCAGTGTGA ACACACCCAA AGAGGCTGCT CCCGAGGAGT TGAGCACCTT TGCGCAGCTT 
AAAGCCTATG TGGAGCTAAA GAGCGAATCG AAACCGGAAC ACCTAATGGA CCAGCGGGAG 
GAAAACCATC ATAATCTTAC AGGAAATCAT CCAGGAGTCA TGCTCCTGGT GGCCTGCGTT 
TTGTTCATCG TCCTGCTCGC CGGTTTGGCC CACGTCTATC GCTGTGAATT GCCTTGGCAA 
AGGAGCAACC GCTCTGGTCA ATTGCGACCG CATCATCAAA GACACCTAAA CGAAACCGAT 
GATGCGCACA GCTTCCTGCA CTATCAGGGA TCTGTGAACT CCAATGGCGG TGATCCGGCT 
CGCCTGCAAA AGTGGCACCA CAGCACGCGG AGAGAAGCAC CCTACAGCTC CCCGCTGCAC 
AATCTACAAG CGCGGGAACT GCAACAGCAA CGCTGCCAGC AATTCTATAG CTCCTCGCTG 
GCGGACAAGA GCTCCTCCAC CTCGTCTTCC TCGTCGGGAA GCAGTCGCAG TAGCCTGCAC 
TCGCCCAGCA GAGATGACAG CTACTATATA GAGATGGCGC CCAGTAGTCC ACCAGCAGCC 
AACCTGCCCA GTTTGCCCAT GGAACTTTTG GGCAGCCGGA GTAATGCCCT CGGATGCCGG 
ACGGATCGAG TGGCAGCCAC CGATCTGGGC GGTACGACAG AGGCGGTACC CTCTTCGGCG 
GCGGCCATCA AGTCGGTGAG CAGCAGACTG ATGACGCCCA GTTCGCGGAG GCTGAACATC 
TGGTGACAAA CCGTCTTTGG TCAGCGACAA TACGCACCCT TTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262529.1
CDS	1320..4787
Translation	MVKIVNQHCDLGLALLLAWTWLTRLVVAAHLVDIPTSSRLAAEREEQQLSRQDVGRLSYQSIHRMLRDENEPDSFRGELRYQQKRHKRELELNAPANKLNLTHRDLRTFNSTGGQWKGDFQVITAMDLSSNQLESLSLDNFNQLRQLDLGNNSLEVIPLSLADTNMSLPFVTLDLSCNKFSQISTSFFAQRLPQLKNLNLAHNELLNISRESFYNLLELQTLVLSHNNISDIDYETFLALPNLQYLDLSHNRLSGSAIRALQGIPDLVSLSIAYNPDVGVAMQEFVASWSLKELDASGTGLCQVPAALAQSVRTLKLSDNWLKAINCGDMDSYPLLQYLDLSHSRIAQVEDDALGRLELLESLFLDRNLLMRVPSSLPPSLEHLFLQHNQIMELPPQAFVGLVNLQTLDLSNNRLIFLPPLSLPKLLTLNLESSGVESVSQSIVHTLPQLRDLLLEDNPIKCSDLLGIAEWASPCRSVDAGQSNGASVSGRVDLKTEYLQFHNFYENFSSRECGIRKPENDTKPPSCSLTRASATLTTTPRSMSKVEKSQEAQATATSVEVAAATAATSEKTGIQATSAAQSTAAATTAEREAIATATSSDTTATPTLAAAAAIQSAGNIPAQLTTKTLRPTETTSLAQLQRRQQMPGMPAKTTETPAKNLPSLAQTKATTALPILATRDAATATTEINSDKPTNISGATKTVATSAAEIATPPAIEVPQTILAGKKSDKMPADKAHETLLKYPTRDTSGQVATTPHKHATLQLHVKDRHLIGTPLLMHKGDVLLVDAEQLLLPGTATVADADSEVLDPSQQHQSAEQEKHQSATDKRQADAINGDTKSPAKGHKKKPSLSIKKMTYSTKHAAKTVEDMAATSKTPQHQHSSVNTPKEAAPEELSTFAQLKAYVELKSESKPEHLMDQREENHHNLTGNHPGVMLLVACVLFIVLLAGLAHVYRCELPWQRSNRSGQLRPHHQRHLNETDDAHSFLHYQGSVNSNGGDPARLQKWHHSTRREAPYSSPLHNLQARELQQQRCQQFYSSSLADKSSSTSSSSSGSSRSSLHSPSRDDSYYIEMAPSSPPAANLPSLPMELLGSRSNALGCRTDRVAATDLGGTTEAVPSSAAAIKSVSSRLMTPSSRRLNIW*QTVFGQRQYAPFQ

Target ORF information:

RefSeq Version	NM_001275600.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 2mit, transcript variant B (2mit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275600.2

ORF Insert Sequence:

ATGGTGAAAA TCGTGAACCA ACACTGTGAT CTAGGCCTGG CCCTTCTCCT GGCCTGGACG 
TGGCTAACAA GGCTGGTGGT GGCGGCCCAT CTGGTCGACA TCCCCACCTC ATCACGCCTG 
GCCGCTGAAC GGGAGGAGCA GCAGTTGTCC CGGCAGGACG TAGGACGGCT CAGCTACCAG 
AGTATCCATC GTATGCTGAG GGACGAAAAC GAACCGGATA GCTTTCGGGG GGAATTGCGA 
TACCAACAGA AACGCCACAA AAGGGAGCTG GAGCTGAATG CGCCGGCCAA CAAGCTTAAT 
CTCACCCACC GCGATTTGAG GACATTCAAT AGCACTGGTG GTCAGTGGAA GGGCGACTTT 
CAAGTGATCA CCGCCATGGA TCTGAGCAGC AATCAACTGG AGAGCCTCAG CTTGGACAAC 
TTCAATCAAC TGAGGCAGCT GGACTTGGGA AATAACTCCC TGGAGGTAAT ACCCTTGAGT 
TTGGCAGACA CCAATATGTC ACTACCCTTT GTGACGCTCG ATCTTTCCTG CAACAAATTC 
AGCCAAATTT CTACGAGCTT TTTTGCCCAG CGATTGCCTC AGTTGAAAAA TCTGAATCTG 
GCTCACAATG AATTGCTAAA TATTTCCCGG GAATCATTCT ATAATTTATT GGAACTACAA 
ACGTTAGTTC TCAGTCACAA CAACATCTCG GATATTGACT ATGAAACATT TTTGGCACTA 
CCGAATCTGC AATATCTGGA TTTATCCCAT AACCGCTTGA GTGGATCCGC TATTCGTGCT 
CTGCAAGGAA TTCCGGATTT GGTCAGCCTT TCCATCGCTT ACAATCCAGA TGTGGGAGTG 
GCGATGCAGG AGTTCGTTGC TTCCTGGAGC CTAAAGGAGT TGGATGCCAG TGGCACTGGA 
TTGTGTCAGG TGCCTGCAGC TCTAGCCCAA TCCGTGAGGA CTCTCAAGTT GTCCGACAAC 
TGGCTCAAGG CAATTAATTG CGGTGACATG GACAGCTATC CGCTGCTGCA GTATCTTGAT 
CTCTCGCACT CCCGCATTGC CCAAGTGGAG GACGATGCCT TGGGACGATT GGAGCTCCTC 
GAATCCCTTT TCCTAGACCG TAATCTACTG ATGCGAGTGC CAAGTAGTCT GCCGCCATCG 
CTGGAACACT TATTTCTGCA GCACAATCAG ATAATGGAGC TTCCGCCACA GGCTTTTGTG 
GGATTGGTCA ATCTACAGAC TCTGGACTTA TCCAACAATC GATTGATCTT CCTGCCCCCG 
CTATCGCTCC CCAAATTGCT CACCCTGAAT CTGGAATCGT CAGGGGTGGA GAGCGTTAGC 
CAATCGATAG TGCACACACT GCCACAGTTA AGGGATCTTC TACTGGAGGA CAACCCCATT 
AAGTGCAGCG ATTTGCTGGG CATTGCCGAA TGGGCCAGTC CTTGCAGGTC AGTGGATGCG 
GGTCAATCGA ATGGGGCAAG TGTAAGTGGG CGCGTGGATT TGAAGACGGA GTATCTGCAA 
TTCCACAATT TTTACGAAAA CTTCAGCAGC CGAGAGTGTG GTATAAGAAA ACCGGAAAAT 
GACACAAAGC CGCCTTCTTG CAGCCTAACA AGGGCATCAG CTACATTAAC AACAACACCC 
AGAAGTATGT CAAAAGTTGA AAAGTCACAA GAAGCACAAG CAACAGCAAC ATCAGTGGAA 
GTAGCAGCGG CAACAGCAGC AACATCAGAA AAAACAGGCA TACAAGCAAC ATCAGCAGCA 
CAGTCGACTG CGGCGGCAAC AACAGCAGAA AGAGAAGCAA TTGCAACAGC AACATCAAGC 
GACACCACAG CAACACCAAC ACTCGCAGCA GCAGCAGCAA TACAATCAGC TGGCAACATC 
CCTGCCCAGT TAACCACAAA AACGTTGCGG CCCACAGAAA CAACTTCGTT AGCGCAACTG 
CAGCGTCGGC AACAAATGCC TGGCATGCCG GCCAAAACAA CAGAAACGCC AGCAAAGAAC 
CTGCCAAGTT TGGCCCAGAC AAAGGCAACA ACAGCGCTTC CCATTTTGGC AACACGAGAT 
GCTGCCACAG CAACAACGGA AATAAATTCC GACAAGCCAA CGAACATTAG CGGTGCCACA 
AAAACAGTAG CAACATCAGC TGCAGAAATA GCAACACCAC CAGCAATTGA AGTGCCACAA 
ACCATTTTGG CCGGCAAAAA ATCTGACAAA ATGCCAGCCG ATAAGGCACA CGAGACTTTA 
TTAAAATACC CAACAAGGGA CACATCCGGT CAAGTTGCAA CAACGCCACA CAAACATGCA 
ACACTGCAGC TGCACGTTAA GGATCGACAT CTAATTGGCA CACCGCTGCT GATGCACAAG 
GGCGATGTCC TATTGGTGGA TGCCGAGCAG TTGTTGCTGC CTGGTACGGC CACCGTGGCG 
GATGCGGATT CGGAAGTTCT GGACCCGAGC CAACAACATC AGTCAGCGGA GCAGGAAAAG 
CACCAGTCAG CGACTGATAA GCGGCAAGCG GATGCAATAA ACGGCGACAC GAAGTCGCCG 
GCGAAAGGCC ACAAAAAGAA ACCATCGCTG AGCATCAAGA AGATGACCTA CAGTACCAAA 
CACGCGGCAA AAACCGTGGA GGATATGGCA GCCACCTCGA AGACACCGCA ACACCAACAT 
AGCAGTGTGA ACACACCCAA AGAGGCTGCT CCCGAGGAGT TGAGCACCTT TGCGCAGCTT 
AAAGCCTATG TGGAGCTAAA GAGCGAATCG AAACCGGAAC ACCTAATGGA CCAGCGGGAG 
GAAAACCATC ATAATCTTAC AGGAAATCAT CCAGGAGTCA TGCTCCTGGT GGCCTGCGTT 
TTGTTCATCG TCCTGCTCGC CGGTTTGGCC CACGTCTATC GCTGTGAATT GCCTTGGCAA 
AGGAGCAACC GCTCTGGTCA ATTGCGACCG CATCATCAAA GACACCTAAA CGAAACCGAT 
GATGCGCACA GCTTCCTGCA CTATCAGGGA TCTGTGAACT CCAATGGCGG TGATCCGGCT 
CGCCTGCAAA AGTGGCACCA CAGCACGCGG AGAGAAGCAC CCTACAGCTC CCCGCTGCAC 
AATCTACAAG CGCGGGAACT GCAACAGCAA CGCTGCCAGC AATTCTATAG CTCCTCGCTG 
GCGGACAAGA GCTCCTCCAC CTCGTCTTCC TCGTCGGGAA GCAGTCGCAG TAGCCTGCAC 
TCGCCCAGCA GAGATGACAG CTACTATATA GAGATGGCGC CCAGTAGTCC ACCAGCAGCC 
AACCTGCCCA GTTTGCCCAT GGAACTTTTG GGCAGCCGGA GTAATGCCCT CGGATGCCGG 
ACGGATCGAG TGGCAGCCAC CGATCTGGGC GGTACGACAG AGGCGGTACC CTCTTCGGCG 
GCGGCCATCA AGTCGGTGAG CAGCAGACTG ATGACGCCCA GTTCGCGGAG GCTGAACATC 
TGGTGACAAA CCGTCTTTGG TCAGCGACAA TACGCACCCT TTCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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2mit cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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