NPF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPF
Entrez Gene ID	42018
Full Name	neuropeptide F
Synonyms	CG10342,Dm-NPF,DmNPF,Dmel\CG10342,Drm-NPF,NP-PP,NPF-A1,NPF-A2,NPF89D3,Npf,dNPF,dnpf,npf
General protein information	Preferred Names neuropeptide F Names CG10342 gene product from transcript CG10342-RB CG10342-PA CG10342-PB CG10342-PC NPF-PA NPF-PB NPF-PC NPY-like neuropeptide F long neuropeptide F neuropeptide F 1 neuropeptide-F
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89D5-89D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_080493.3	NP_536741.1	neuropeptide F, isoform A
NM_001275713.1	NP_001262642.1	neuropeptide F, isoform B
NM_001275714.1	NP_001262643.1	neuropeptide F, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data highlight the importance of neuronal clocks for peripheral rhythms, particularly in a specific detoxification pathway, and identify a novel and conserved role for NPF/Npy in circadian rhythms.
Title: Neural clocks and Neuropeptide F/Y regulate circadian gene expression in a peripheral metabolic tissue.

Too much or too little NPF or its shorter isoform, which are key players in homeostatic feeding regulation, leads to increased sensitivity to amino acid starvation and diminished survivorship when compared to controls.
Title: Manipulation of components that control feeding behavior in Drosophila melanogaster increases sensitivity to amino acid starvation.

This study identify a small subset of clock neurons in the male brain that regulate rival-induced Longer-Mating-Duration via neuropeptide signaling.
Title: A PDF/NPF neuropeptide signaling circuitry of male Drosophila melanogaster controls rival-induced prolonged mating.

Distribution of short neuropeptide F and its receptor in neuronal circuits related to feeding in larval Drosophila.
Title: Distribution of short neuropeptide F and its receptor in neuronal circuits related to feeding in larval Drosophila.

Male-specific sex-dependent regulatory function of sleep is controlled by NPF and NPFR1 expression in the brain.
Title: Regulation of sleep by neuropeptide Y-like system in Drosophila melanogaster.

The following NPF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05143	NM_001275713.1 Latest version!	Drosophila melanogaster neuropeptide F (NPF), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa05143	NM_001275714.1 Latest version!	Drosophila melanogaster neuropeptide F (NPF), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa05143	NM_080493.3 Latest version!	Drosophila melanogaster neuropeptide F (NPF), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPF ( NM_080493.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_536741.1 Drosophila melanogaster neuropeptide F (NPF), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05143
Clone ID Related Accession (Same CDS sequence)	NM_080493.3 , NM_001275713.1 , NM_001275714.1
Accession Version	NM_080493.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuropeptide F, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_080493.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCCAAA CAATGCGTTG CATCCTGGTT GCCTGTGTGG CCCTTGCCCT CCTAGCCGCC 
GGCTGCCGAG TGGAGGCGTC CAACTCCAGA CCTCCGCGAA AGAACGATGT CAACACTATG 
GCTGATGCCT ACAAGTTCCT GCAGGATCTG GACACCTACT ACGGCGACAG AGCCCGCGTT 
CGGTTCGGAA AGCGCGGATC GCTGATGGAT ATCCTGAGGA ATCACGAGAT GGACAACATA 
AATCTAGGAA AAAATGCCAA CAATGGAGGA GAATTTGCTC GCGGTTTTAA TGAGGAGGAG 
ATATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536741.1
CDS	76..384
Translation	MCQTMRCILVACVALALLAAGCRVEASNSRPPRKNDVNTMADAYKFLQDLDTYYGDRARVRFGKRGSLMDILRNHEMDNINLGKNANNGGEFARGFNEEEIF

Target ORF information:

RefSeq Version	NM_080493.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuropeptide F (NPF), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080493.3

ORF Insert Sequence:

ATGTGCCAAA CAATGCGTTG CATCCTGGTT GCCTGTGTGG CCCTTGCCCT CCTAGCCGCC 
GGCTGCCGAG TGGAGGCGTC CAACTCCAGA CCTCCGCGAA AGAACGATGT CAACACTATG 
GCTGATGCCT ACAAGTTCCT GCAGGATCTG GACACCTACT ACGGCGACAG AGCCCGCGTT 
CGGTTCGGAA AGCGCGGATC GCTGATGGAT ATCCTGAGGA ATCACGAGAT GGACAACATA 
AATCTAGGAA AAAATGCCAA CAATGGAGGA GAATTTGCTC GCGGTTTTAA TGAGGAGGAG 
ATATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPF ( NM_001275713.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262642.1 Drosophila melanogaster neuropeptide F (NPF), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05143
Clone ID Related Accession (Same CDS sequence)	NM_080493.3 , NM_001275713.1 , NM_001275714.1
Accession Version	NM_001275713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuropeptide F, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCCAAA CAATGCGTTG CATCCTGGTT GCCTGTGTGG CCCTTGCCCT CCTAGCCGCC 
GGCTGCCGAG TGGAGGCGTC CAACTCCAGA CCTCCGCGAA AGAACGATGT CAACACTATG 
GCTGATGCCT ACAAGTTCCT GCAGGATCTG GACACCTACT ACGGCGACAG AGCCCGCGTT 
CGGTTCGGAA AGCGCGGATC GCTGATGGAT ATCCTGAGGA ATCACGAGAT GGACAACATA 
AATCTAGGAA AAAATGCCAA CAATGGAGGA GAATTTGCTC GCGGTTTTAA TGAGGAGGAG 
ATATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262642.1
CDS	25..333
Translation	MCQTMRCILVACVALALLAAGCRVEASNSRPPRKNDVNTMADAYKFLQDLDTYYGDRARVRFGKRGSLMDILRNHEMDNINLGKNANNGGEFARGFNEEEIF

Target ORF information:

RefSeq Version	NM_001275713.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuropeptide F (NPF), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275713.1

ORF Insert Sequence:

ATGTGCCAAA CAATGCGTTG CATCCTGGTT GCCTGTGTGG CCCTTGCCCT CCTAGCCGCC 
GGCTGCCGAG TGGAGGCGTC CAACTCCAGA CCTCCGCGAA AGAACGATGT CAACACTATG 
GCTGATGCCT ACAAGTTCCT GCAGGATCTG GACACCTACT ACGGCGACAG AGCCCGCGTT 
CGGTTCGGAA AGCGCGGATC GCTGATGGAT ATCCTGAGGA ATCACGAGAT GGACAACATA 
AATCTAGGAA AAAATGCCAA CAATGGAGGA GAATTTGCTC GCGGTTTTAA TGAGGAGGAG 
ATATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPF ( NM_001275714.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262643.1 Drosophila melanogaster neuropeptide F (NPF), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05143
Clone ID Related Accession (Same CDS sequence)	NM_080493.3 , NM_001275713.1 , NM_001275714.1
Accession Version	NM_001275714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neuropeptide F, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCCAAA CAATGCGTTG CATCCTGGTT GCCTGTGTGG CCCTTGCCCT CCTAGCCGCC 
GGCTGCCGAG TGGAGGCGTC CAACTCCAGA CCTCCGCGAA AGAACGATGT CAACACTATG 
GCTGATGCCT ACAAGTTCCT GCAGGATCTG GACACCTACT ACGGCGACAG AGCCCGCGTT 
CGGTTCGGAA AGCGCGGATC GCTGATGGAT ATCCTGAGGA ATCACGAGAT GGACAACATA 
AATCTAGGAA AAAATGCCAA CAATGGAGGA GAATTTGCTC GCGGTTTTAA TGAGGAGGAG 
ATATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262643.1
CDS	86..394
Translation	MCQTMRCILVACVALALLAAGCRVEASNSRPPRKNDVNTMADAYKFLQDLDTYYGDRARVRFGKRGSLMDILRNHEMDNINLGKNANNGGEFARGFNEEEIF

Target ORF information:

RefSeq Version	NM_001275714.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neuropeptide F (NPF), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275714.1

ORF Insert Sequence:

ATGTGCCAAA CAATGCGTTG CATCCTGGTT GCCTGTGTGG CCCTTGCCCT CCTAGCCGCC 
GGCTGCCGAG TGGAGGCGTC CAACTCCAGA CCTCCGCGAA AGAACGATGT CAACACTATG 
GCTGATGCCT ACAAGTTCCT GCAGGATCTG GACACCTACT ACGGCGACAG AGCCCGCGTT 
CGGTTCGGAA AGCGCGGATC GCTGATGGAT ATCCTGAGGA ATCACGAGAT GGACAACATA 
AATCTAGGAA AAAATGCCAA CAATGGAGGA GAATTTGCTC GCGGTTTTAA TGAGGAGGAG 
ATATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NPF cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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ORF Insert Sequence:

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