htl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	htl
Entrez Gene ID	42160
Full Name	heartless
Synonyms	CG7223,CT22273,CT39172,DFGF-R1,DFGF-R2,DFR-1,DFR1,DFR1/DFGF-R2,DPR3,DTRK(FR1),Dfr-1,Dfr1,DmHD-38,Dmel\CG7223,Dtk1,EMS2,FGF-R2,FGFR,FGFR2,FR1,Fr1,HD-38,HTL/FGFR1,Htl,Tk1,dtk1,i100,i150,i79,j372
General protein information	Preferred Names heartless Names CG7223 gene product from transcript CG7223-RB CG7223-PA CG7223-PB CG7223-PC FGF receptor fibroblast growth factor receptor fibroblast growth factor receptor 1 htl-PA htl-PB htl-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 90E2-90E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_169784.2	NP_732286.1	heartless, isoform A
NM_169785.2	NP_732287.1	heartless, isoform C
NM_079670.3	NP_524394.2	heartless, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FGFR3	XP_005618584.1
Drosophila melanogaster (fruit fly)	htl	NP_732286.1
Xenopus tropicalis (tropical clawed frog)	fgfr3	NP_001135467.1
	FGFR3	XP_001101108.1
Mus musculus (house mouse)	Fgfr3	NP_001156687.1
Gallus gallus (chicken)	FGFR3	NP_990840.2
Danio rerio (zebrafish)	fgfr3	NP_571681.1
Caenorhabditis elegans (roundworm)	egl-15	NP_001024723.1
Homo sapiens (human)	FGFR3	NP_000133.1
Pan troglodytes (chimpanzee)	FGFR3	XP_003310269.1
Bos taurus (cattle)	FGFR3	NP_776743.1
Rattus norvegicus (Norway rat)	Fgfr3	NP_445881.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mactosylceramide, an early product in GSL biosynthesis, prevents inappropriate activation of insulin and fibroblast growth factor receptors in Drosophila glial cells and hypertrophy.
Title: Mactosylceramide prevents glial cell overgrowth by inhibiting insulin and fibroblast growth factor receptor signaling.

Proliferation of epithelial sheath progenitors, the apical cells, was decreased in both htl and ths mutants, while ectopic expression of the Ths ligand led to these cells' over-proliferation suggesting that FGF signaling supports ovarian muscle sheath formation by controlling apical cell number in the developing gonad.
Title: FGF signaling supports Drosophila fertility by regulating development of ovarian muscle tissues.

Our data identify FGF signaling through Heartless as a key regulator of astrocyte morphological elaboration in vivo.
Title: Neuron-glia interactions through the Heartless FGF receptor signaling pathway mediate morphogenesis of Drosophila astrocytes.

identify two transcriptional regulators that function downstream of Heartless signaling in lymph gland progenitors, the ETS protein, Pointed, and the Friend-of-GATA protein, U-shaped, which are required for this Heartless-induced differentiation response
Title: Extracellular matrix-modulated Heartless signaling in Drosophila blood progenitors regulates their differentiation via a Ras/ETS/FOG pathway and target of rapamycin function.

Mesodermal cell spreading is dependent on the FGF receptor Heartless.
Title: Photoactivatable GFP resolves Drosophila mesoderm migration behaviour.

The following htl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the htl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08456	NM_169784.2 Latest version!	Drosophila melanogaster heartless (htl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08456	NM_169785.2 Latest version!	Drosophila melanogaster heartless (htl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08456	NM_079670.3 Latest version!	Drosophila melanogaster heartless (htl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

htl ( NM_169784.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732286.1 Drosophila melanogaster heartless (htl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08456
Clone ID Related Accession (Same CDS sequence)	NM_169784.2 , NM_169785.2 , NM_079670.3
Accession Version	NM_169784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heartless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169784.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCCG CCTGGAGTTG GCGAGCCAGT CACTCAACGA TCACAATGAC AAGCGGATCG 
CTGGTAGTGC TCTTCCTGTT GCTTTCGATT TGGCAGCCAG CCGTGCAGGT TGAAGGACGA 
CGCCAAATGG CCAATAGCCA GGAGATGATC AAGGATCATT TGGGAGCCAG GAGTCAAAAC 
AAGACACCAG CCATCACGAA CAATGCTAAT CAATCGAGCA CATCCAGTGC GGATTTGGAT 
GATGGCGCCG CAGATGATGA TGATAACAAG GCCGATTTGC CGGTGAATGT CAGTTCGAAA 
CCCTACTGGC GTAATCCCAA AAAGATGAGT TTTCTGCAGA CGCGACCCTC GGGCTCACTG 
CTCACCCTCA ATTGCCATGC CCTGGGCAAT CCTGAGCCCA ATATCACATG GTATCGCAAT 
GGCACCGTCG ACTGGACGCG CGGCTATGGG AGCCTGAAGC GGAATCGATG GACCCTGACG 
ATGGAGGATC TGGTGCCGGG CGATTGTGGC AACTACACCT GCAAGGTGTG CAATTCATTG 
GGTTGCATTC GTCACGACAC CCAGGTGATT GTAAGCGATC GAGTTAATCA CAAACCGATC 
CTGATGACCG GTCCATTGAA TCTCACACTG GTGGTCAATT CCACAGGCAG CATGCACTGC 
AAATATCTAT CGGACTTGAC CAGCAAGAAG GCGTGGATCT TTGTGCCCTG CCATGGCATG 
ACCAACTGCT CCAACAATCG ATCCATCATT GCCGAGGATA AGGATCAGTT GGACTTCGTG 
AACGTTAGGA TGGAGCAGGA GGGCTGGTAC ACGTGCGTCG AAAGCAACAG CCTGGGTCAA 
TCCAATAGCA CTGCATATCT GCGAGTGGTG CGAAGTCTTC ACGTCTTGGA AGCCGGAGTT 
GCCAGTGGTT CACTTCACTC GACCAGCTTC GTGTATATCT TCGTCTTTGG CGGACTTATT 
TTCATCTTTA TGACCACCCT GTTTGTGTTC TATGCCATCC GAAAGATGAA ACATGAAAAG 
GTGTTGAAAC AACGCATCGA AACCGTTCAC CAGTGGACCA AGAAAGTGAT CATCTTCAAG 
CCCGAAGGTG GCGGAGACTC CAGTGGTTCC ATGGACACCA TGATTATGCC GGTGGTTAGG 
ATACAGAAAC AGCGCACCAC TGTTCTTCAG AATGGCAACG AGCCGGCTCC ATTCAATGAA 
TATGAATTTC CACTGGACTC GAACTGGGAA CTGCCCAGAA GTCATTTGGT ACTGGGTGCC 
ACTTTGGGAG AAGGTGCTTT CGGACGAGTG GTCATGGCGG AGGTCAATAA TGCCATTGTC 
GCCGTGAAAA TGGTGAAGGA AGGACACACG GATGATGACA TTGCCAGCTT GGTGCGGGAA 
ATGGAAGTGA TGAAGATCAT TGGGCGACAT ATCAATATTA TTAACTTACT TGGTTGCTGC 
AGTCAAAATG GTCCGCTCTA TGTGATTGTC GAGTATGCGC CACACGGAAA TCTCAAGGAC 
TTCCTCTATA AAAATCGACC CTTCGGAAGG GATCAGGATA GGGACAGCTC GCAACCGCCG 
CCATCGCCAC CAGCTCATGT GATAACCGAA AAGGATCTGA TCAAATTTGC CCACCAAATT 
GCCAGAGGAA TGGACTATTT GGCCTCGCGG CGATGCATCC ATCGAGATTT GGCAGCCAGG 
AATGTGCTCG TCAGCGATGA TTATGTGCTG AAGATTGCTG ATTTTGGACT GGCGAGGGAC 
ATTCAAAGCA CGGATTACTA TCGGAAGAAC ACAAATGGCA GGCTACCCAT CAAATGGATG 
GCACCGGAGT CGCTGCAGGA GAAATTCTAT GATTCCAAGA GCGATGTCTG GTCATATGGC 
ATCCTGCTGT GGGAGATCAT GACCTATGGG CAGCAACCAT ATCCAACTAT CATGTCCGCT 
GAGGAGCTGT ACACCTATCT CATGTCCGGT CAGCGGATGG AGAAACCAGC GAAATGCTCC 
ATGAACATCT ACATTCTGAT GCGACAATGT TGGCATTTCA ACGCCGACGA TCGGCCACCT 
TTTACGGAAA TTGTTGAGTA TATGGACAAG CTGCTCCAGA CGAAGGAGGA CTACCTCGAT 
GTGGATATCG CCAATCTGGA TACACCGCCC TCGACTAGCG ACGAGGAGGA AGATGAAACG 
GACAACCTGC AGAAGTGGTG TAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732286.1
CDS	907..3096
Translation	MAAAWSWRASHSTITMTSGSLVVLFLLLSIWQPAVQVEGRRQMANSQEMIKDHLGARSQNKTPAITNNANQSSTSSADLDDGAADDDDNKADLPVNVSSKPYWRNPKKMSFLQTRPSGSLLTLNCHALGNPEPNITWYRNGTVDWTRGYGSLKRNRWTLTMEDLVPGDCGNYTCKVCNSLGCIRHDTQVIVSDRVNHKPILMTGPLNLTLVVNSTGSMHCKYLSDLTSKKAWIFVPCHGMTNCSNNRSIIAEDKDQLDFVNVRMEQEGWYTCVESNSLGQSNSTAYLRVVRSLHVLEAGVASGSLHSTSFVYIFVFGGLIFIFMTTLFVFYAIRKMKHEKVLKQRIETVHQWTKKVIIFKPEGGGDSSGSMDTMIMPVVRIQKQRTTVLQNGNEPAPFNEYEFPLDSNWELPRSHLVLGATLGEGAFGRVVMAEVNNAIVAVKMVKEGHTDDDIASLVREMEVMKIIGRHINIINLLGCCSQNGPLYVIVEYAPHGNLKDFLYKNRPFGRDQDRDSSQPPPSPPAHVITEKDLIKFAHQIARGMDYLASRRCIHRDLAARNVLVSDDYVLKIADFGLARDIQSTDYYRKNTNGRLPIKWMAPESLQEKFYDSKSDVWSYGILLWEIMTYGQQPYPTIMSAEELYTYLMSGQRMEKPAKCSMNIYILMRQCWHFNADDRPPFTEIVEYMDKLLQTKEDYLDVDIANLDTPPSTSDEEEDETDNLQKWCNY

Target ORF information:

RefSeq Version	NM_169784.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heartless (htl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169784.2

ORF Insert Sequence:

ATGGCTGCCG CCTGGAGTTG GCGAGCCAGT CACTCAACGA TCACAATGAC AAGCGGATCG 
CTGGTAGTGC TCTTCCTGTT GCTTTCGATT TGGCAGCCAG CCGTGCAGGT TGAAGGACGA 
CGCCAAATGG CCAATAGCCA GGAGATGATC AAGGATCATT TGGGAGCCAG GAGTCAAAAC 
AAGACACCAG CCATCACGAA CAATGCTAAT CAATCGAGCA CATCCAGTGC GGATTTGGAT 
GATGGCGCCG CAGATGATGA TGATAACAAG GCCGATTTGC CGGTGAATGT CAGTTCGAAA 
CCCTACTGGC GTAATCCCAA AAAGATGAGT TTTCTGCAGA CGCGACCCTC GGGCTCACTG 
CTCACCCTCA ATTGCCATGC CCTGGGCAAT CCTGAGCCCA ATATCACATG GTATCGCAAT 
GGCACCGTCG ACTGGACGCG CGGCTATGGG AGCCTGAAGC GGAATCGATG GACCCTGACG 
ATGGAGGATC TGGTGCCGGG CGATTGTGGC AACTACACCT GCAAGGTGTG CAATTCATTG 
GGTTGCATTC GTCACGACAC CCAGGTGATT GTAAGCGATC GAGTTAATCA CAAACCGATC 
CTGATGACCG GTCCATTGAA TCTCACACTG GTGGTCAATT CCACAGGCAG CATGCACTGC 
AAATATCTAT CGGACTTGAC CAGCAAGAAG GCGTGGATCT TTGTGCCCTG CCATGGCATG 
ACCAACTGCT CCAACAATCG ATCCATCATT GCCGAGGATA AGGATCAGTT GGACTTCGTG 
AACGTTAGGA TGGAGCAGGA GGGCTGGTAC ACGTGCGTCG AAAGCAACAG CCTGGGTCAA 
TCCAATAGCA CTGCATATCT GCGAGTGGTG CGAAGTCTTC ACGTCTTGGA AGCCGGAGTT 
GCCAGTGGTT CACTTCACTC GACCAGCTTC GTGTATATCT TCGTCTTTGG CGGACTTATT 
TTCATCTTTA TGACCACCCT GTTTGTGTTC TATGCCATCC GAAAGATGAA ACATGAAAAG 
GTGTTGAAAC AACGCATCGA AACCGTTCAC CAGTGGACCA AGAAAGTGAT CATCTTCAAG 
CCCGAAGGTG GCGGAGACTC CAGTGGTTCC ATGGACACCA TGATTATGCC GGTGGTTAGG 
ATACAGAAAC AGCGCACCAC TGTTCTTCAG AATGGCAACG AGCCGGCTCC ATTCAATGAA 
TATGAATTTC CACTGGACTC GAACTGGGAA CTGCCCAGAA GTCATTTGGT ACTGGGTGCC 
ACTTTGGGAG AAGGTGCTTT CGGACGAGTG GTCATGGCGG AGGTCAATAA TGCCATTGTC 
GCCGTGAAAA TGGTGAAGGA AGGACACACG GATGATGACA TTGCCAGCTT GGTGCGGGAA 
ATGGAAGTGA TGAAGATCAT TGGGCGACAT ATCAATATTA TTAACTTACT TGGTTGCTGC 
AGTCAAAATG GTCCGCTCTA TGTGATTGTC GAGTATGCGC CACACGGAAA TCTCAAGGAC 
TTCCTCTATA AAAATCGACC CTTCGGAAGG GATCAGGATA GGGACAGCTC GCAACCGCCG 
CCATCGCCAC CAGCTCATGT GATAACCGAA AAGGATCTGA TCAAATTTGC CCACCAAATT 
GCCAGAGGAA TGGACTATTT GGCCTCGCGG CGATGCATCC ATCGAGATTT GGCAGCCAGG 
AATGTGCTCG TCAGCGATGA TTATGTGCTG AAGATTGCTG ATTTTGGACT GGCGAGGGAC 
ATTCAAAGCA CGGATTACTA TCGGAAGAAC ACAAATGGCA GGCTACCCAT CAAATGGATG 
GCACCGGAGT CGCTGCAGGA GAAATTCTAT GATTCCAAGA GCGATGTCTG GTCATATGGC 
ATCCTGCTGT GGGAGATCAT GACCTATGGG CAGCAACCAT ATCCAACTAT CATGTCCGCT 
GAGGAGCTGT ACACCTATCT CATGTCCGGT CAGCGGATGG AGAAACCAGC GAAATGCTCC 
ATGAACATCT ACATTCTGAT GCGACAATGT TGGCATTTCA ACGCCGACGA TCGGCCACCT 
TTTACGGAAA TTGTTGAGTA TATGGACAAG CTGCTCCAGA CGAAGGAGGA CTACCTCGAT 
GTGGATATCG CCAATCTGGA TACACCGCCC TCGACTAGCG ACGAGGAGGA AGATGAAACG 
GACAACCTGC AGAAGTGGTG TAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

htl ( NM_169785.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732287.1 Drosophila melanogaster heartless (htl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08456
Clone ID Related Accession (Same CDS sequence)	NM_169784.2 , NM_169785.2 , NM_079670.3
Accession Version	NM_169785.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heartless, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169785.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCCG CCTGGAGTTG GCGAGCCAGT CACTCAACGA TCACAATGAC AAGCGGATCG 
CTGGTAGTGC TCTTCCTGTT GCTTTCGATT TGGCAGCCAG CCGTGCAGGT TGAAGGACGA 
CGCCAAATGG CCAATAGCCA GGAGATGATC AAGGATCATT TGGGAGCCAG GAGTCAAAAC 
AAGACACCAG CCATCACGAA CAATGCTAAT CAATCGAGCA CATCCAGTGC GGATTTGGAT 
GATGGCGCCG CAGATGATGA TGATAACAAG GCCGATTTGC CGGTGAATGT CAGTTCGAAA 
CCCTACTGGC GTAATCCCAA AAAGATGAGT TTTCTGCAGA CGCGACCCTC GGGCTCACTG 
CTCACCCTCA ATTGCCATGC CCTGGGCAAT CCTGAGCCCA ATATCACATG GTATCGCAAT 
GGCACCGTCG ACTGGACGCG CGGCTATGGG AGCCTGAAGC GGAATCGATG GACCCTGACG 
ATGGAGGATC TGGTGCCGGG CGATTGTGGC AACTACACCT GCAAGGTGTG CAATTCATTG 
GGTTGCATTC GTCACGACAC CCAGGTGATT GTAAGCGATC GAGTTAATCA CAAACCGATC 
CTGATGACCG GTCCATTGAA TCTCACACTG GTGGTCAATT CCACAGGCAG CATGCACTGC 
AAATATCTAT CGGACTTGAC CAGCAAGAAG GCGTGGATCT TTGTGCCCTG CCATGGCATG 
ACCAACTGCT CCAACAATCG ATCCATCATT GCCGAGGATA AGGATCAGTT GGACTTCGTG 
AACGTTAGGA TGGAGCAGGA GGGCTGGTAC ACGTGCGTCG AAAGCAACAG CCTGGGTCAA 
TCCAATAGCA CTGCATATCT GCGAGTGGTG CGAAGTCTTC ACGTCTTGGA AGCCGGAGTT 
GCCAGTGGTT CACTTCACTC GACCAGCTTC GTGTATATCT TCGTCTTTGG CGGACTTATT 
TTCATCTTTA TGACCACCCT GTTTGTGTTC TATGCCATCC GAAAGATGAA ACATGAAAAG 
GTGTTGAAAC AACGCATCGA AACCGTTCAC CAGTGGACCA AGAAAGTGAT CATCTTCAAG 
CCCGAAGGTG GCGGAGACTC CAGTGGTTCC ATGGACACCA TGATTATGCC GGTGGTTAGG 
ATACAGAAAC AGCGCACCAC TGTTCTTCAG AATGGCAACG AGCCGGCTCC ATTCAATGAA 
TATGAATTTC CACTGGACTC GAACTGGGAA CTGCCCAGAA GTCATTTGGT ACTGGGTGCC 
ACTTTGGGAG AAGGTGCTTT CGGACGAGTG GTCATGGCGG AGGTCAATAA TGCCATTGTC 
GCCGTGAAAA TGGTGAAGGA AGGACACACG GATGATGACA TTGCCAGCTT GGTGCGGGAA 
ATGGAAGTGA TGAAGATCAT TGGGCGACAT ATCAATATTA TTAACTTACT TGGTTGCTGC 
AGTCAAAATG GTCCGCTCTA TGTGATTGTC GAGTATGCGC CACACGGAAA TCTCAAGGAC 
TTCCTCTATA AAAATCGACC CTTCGGAAGG GATCAGGATA GGGACAGCTC GCAACCGCCG 
CCATCGCCAC CAGCTCATGT GATAACCGAA AAGGATCTGA TCAAATTTGC CCACCAAATT 
GCCAGAGGAA TGGACTATTT GGCCTCGCGG CGATGCATCC ATCGAGATTT GGCAGCCAGG 
AATGTGCTCG TCAGCGATGA TTATGTGCTG AAGATTGCTG ATTTTGGACT GGCGAGGGAC 
ATTCAAAGCA CGGATTACTA TCGGAAGAAC ACAAATGGCA GGCTACCCAT CAAATGGATG 
GCACCGGAGT CGCTGCAGGA GAAATTCTAT GATTCCAAGA GCGATGTCTG GTCATATGGC 
ATCCTGCTGT GGGAGATCAT GACCTATGGG CAGCAACCAT ATCCAACTAT CATGTCCGCT 
GAGGAGCTGT ACACCTATCT CATGTCCGGT CAGCGGATGG AGAAACCAGC GAAATGCTCC 
ATGAACATCT ACATTCTGAT GCGACAATGT TGGCATTTCA ACGCCGACGA TCGGCCACCT 
TTTACGGAAA TTGTTGAGTA TATGGACAAG CTGCTCCAGA CGAAGGAGGA CTACCTCGAT 
GTGGATATCG CCAATCTGGA TACACCGCCC TCGACTAGCG ACGAGGAGGA AGATGAAACG 
GACAACCTGC AGAAGTGGTG TAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732287.1
CDS	788..2977
Translation	MAAAWSWRASHSTITMTSGSLVVLFLLLSIWQPAVQVEGRRQMANSQEMIKDHLGARSQNKTPAITNNANQSSTSSADLDDGAADDDDNKADLPVNVSSKPYWRNPKKMSFLQTRPSGSLLTLNCHALGNPEPNITWYRNGTVDWTRGYGSLKRNRWTLTMEDLVPGDCGNYTCKVCNSLGCIRHDTQVIVSDRVNHKPILMTGPLNLTLVVNSTGSMHCKYLSDLTSKKAWIFVPCHGMTNCSNNRSIIAEDKDQLDFVNVRMEQEGWYTCVESNSLGQSNSTAYLRVVRSLHVLEAGVASGSLHSTSFVYIFVFGGLIFIFMTTLFVFYAIRKMKHEKVLKQRIETVHQWTKKVIIFKPEGGGDSSGSMDTMIMPVVRIQKQRTTVLQNGNEPAPFNEYEFPLDSNWELPRSHLVLGATLGEGAFGRVVMAEVNNAIVAVKMVKEGHTDDDIASLVREMEVMKIIGRHINIINLLGCCSQNGPLYVIVEYAPHGNLKDFLYKNRPFGRDQDRDSSQPPPSPPAHVITEKDLIKFAHQIARGMDYLASRRCIHRDLAARNVLVSDDYVLKIADFGLARDIQSTDYYRKNTNGRLPIKWMAPESLQEKFYDSKSDVWSYGILLWEIMTYGQQPYPTIMSAEELYTYLMSGQRMEKPAKCSMNIYILMRQCWHFNADDRPPFTEIVEYMDKLLQTKEDYLDVDIANLDTPPSTSDEEEDETDNLQKWCNY

Target ORF information:

RefSeq Version	NM_169785.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heartless (htl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169785.2

ORF Insert Sequence:

ATGGCTGCCG CCTGGAGTTG GCGAGCCAGT CACTCAACGA TCACAATGAC AAGCGGATCG 
CTGGTAGTGC TCTTCCTGTT GCTTTCGATT TGGCAGCCAG CCGTGCAGGT TGAAGGACGA 
CGCCAAATGG CCAATAGCCA GGAGATGATC AAGGATCATT TGGGAGCCAG GAGTCAAAAC 
AAGACACCAG CCATCACGAA CAATGCTAAT CAATCGAGCA CATCCAGTGC GGATTTGGAT 
GATGGCGCCG CAGATGATGA TGATAACAAG GCCGATTTGC CGGTGAATGT CAGTTCGAAA 
CCCTACTGGC GTAATCCCAA AAAGATGAGT TTTCTGCAGA CGCGACCCTC GGGCTCACTG 
CTCACCCTCA ATTGCCATGC CCTGGGCAAT CCTGAGCCCA ATATCACATG GTATCGCAAT 
GGCACCGTCG ACTGGACGCG CGGCTATGGG AGCCTGAAGC GGAATCGATG GACCCTGACG 
ATGGAGGATC TGGTGCCGGG CGATTGTGGC AACTACACCT GCAAGGTGTG CAATTCATTG 
GGTTGCATTC GTCACGACAC CCAGGTGATT GTAAGCGATC GAGTTAATCA CAAACCGATC 
CTGATGACCG GTCCATTGAA TCTCACACTG GTGGTCAATT CCACAGGCAG CATGCACTGC 
AAATATCTAT CGGACTTGAC CAGCAAGAAG GCGTGGATCT TTGTGCCCTG CCATGGCATG 
ACCAACTGCT CCAACAATCG ATCCATCATT GCCGAGGATA AGGATCAGTT GGACTTCGTG 
AACGTTAGGA TGGAGCAGGA GGGCTGGTAC ACGTGCGTCG AAAGCAACAG CCTGGGTCAA 
TCCAATAGCA CTGCATATCT GCGAGTGGTG CGAAGTCTTC ACGTCTTGGA AGCCGGAGTT 
GCCAGTGGTT CACTTCACTC GACCAGCTTC GTGTATATCT TCGTCTTTGG CGGACTTATT 
TTCATCTTTA TGACCACCCT GTTTGTGTTC TATGCCATCC GAAAGATGAA ACATGAAAAG 
GTGTTGAAAC AACGCATCGA AACCGTTCAC CAGTGGACCA AGAAAGTGAT CATCTTCAAG 
CCCGAAGGTG GCGGAGACTC CAGTGGTTCC ATGGACACCA TGATTATGCC GGTGGTTAGG 
ATACAGAAAC AGCGCACCAC TGTTCTTCAG AATGGCAACG AGCCGGCTCC ATTCAATGAA 
TATGAATTTC CACTGGACTC GAACTGGGAA CTGCCCAGAA GTCATTTGGT ACTGGGTGCC 
ACTTTGGGAG AAGGTGCTTT CGGACGAGTG GTCATGGCGG AGGTCAATAA TGCCATTGTC 
GCCGTGAAAA TGGTGAAGGA AGGACACACG GATGATGACA TTGCCAGCTT GGTGCGGGAA 
ATGGAAGTGA TGAAGATCAT TGGGCGACAT ATCAATATTA TTAACTTACT TGGTTGCTGC 
AGTCAAAATG GTCCGCTCTA TGTGATTGTC GAGTATGCGC CACACGGAAA TCTCAAGGAC 
TTCCTCTATA AAAATCGACC CTTCGGAAGG GATCAGGATA GGGACAGCTC GCAACCGCCG 
CCATCGCCAC CAGCTCATGT GATAACCGAA AAGGATCTGA TCAAATTTGC CCACCAAATT 
GCCAGAGGAA TGGACTATTT GGCCTCGCGG CGATGCATCC ATCGAGATTT GGCAGCCAGG 
AATGTGCTCG TCAGCGATGA TTATGTGCTG AAGATTGCTG ATTTTGGACT GGCGAGGGAC 
ATTCAAAGCA CGGATTACTA TCGGAAGAAC ACAAATGGCA GGCTACCCAT CAAATGGATG 
GCACCGGAGT CGCTGCAGGA GAAATTCTAT GATTCCAAGA GCGATGTCTG GTCATATGGC 
ATCCTGCTGT GGGAGATCAT GACCTATGGG CAGCAACCAT ATCCAACTAT CATGTCCGCT 
GAGGAGCTGT ACACCTATCT CATGTCCGGT CAGCGGATGG AGAAACCAGC GAAATGCTCC 
ATGAACATCT ACATTCTGAT GCGACAATGT TGGCATTTCA ACGCCGACGA TCGGCCACCT 
TTTACGGAAA TTGTTGAGTA TATGGACAAG CTGCTCCAGA CGAAGGAGGA CTACCTCGAT 
GTGGATATCG CCAATCTGGA TACACCGCCC TCGACTAGCG ACGAGGAGGA AGATGAAACG 
GACAACCTGC AGAAGTGGTG TAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

htl ( NM_079670.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524394.2 Drosophila melanogaster heartless (htl), transcript variant B, mRNA.

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CloneID	OFa08456
Clone ID Related Accession (Same CDS sequence)	NM_169784.2 , NM_169785.2 , NM_079670.3
Accession Version	NM_079670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heartless, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079670.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCCG CCTGGAGTTG GCGAGCCAGT CACTCAACGA TCACAATGAC AAGCGGATCG 
CTGGTAGTGC TCTTCCTGTT GCTTTCGATT TGGCAGCCAG CCGTGCAGGT TGAAGGACGA 
CGCCAAATGG CCAATAGCCA GGAGATGATC AAGGATCATT TGGGAGCCAG GAGTCAAAAC 
AAGACACCAG CCATCACGAA CAATGCTAAT CAATCGAGCA CATCCAGTGC GGATTTGGAT 
GATGGCGCCG CAGATGATGA TGATAACAAG GCCGATTTGC CGGTGAATGT CAGTTCGAAA 
CCCTACTGGC GTAATCCCAA AAAGATGAGT TTTCTGCAGA CGCGACCCTC GGGCTCACTG 
CTCACCCTCA ATTGCCATGC CCTGGGCAAT CCTGAGCCCA ATATCACATG GTATCGCAAT 
GGCACCGTCG ACTGGACGCG CGGCTATGGG AGCCTGAAGC GGAATCGATG GACCCTGACG 
ATGGAGGATC TGGTGCCGGG CGATTGTGGC AACTACACCT GCAAGGTGTG CAATTCATTG 
GGTTGCATTC GTCACGACAC CCAGGTGATT GTAAGCGATC GAGTTAATCA CAAACCGATC 
CTGATGACCG GTCCATTGAA TCTCACACTG GTGGTCAATT CCACAGGCAG CATGCACTGC 
AAATATCTAT CGGACTTGAC CAGCAAGAAG GCGTGGATCT TTGTGCCCTG CCATGGCATG 
ACCAACTGCT CCAACAATCG ATCCATCATT GCCGAGGATA AGGATCAGTT GGACTTCGTG 
AACGTTAGGA TGGAGCAGGA GGGCTGGTAC ACGTGCGTCG AAAGCAACAG CCTGGGTCAA 
TCCAATAGCA CTGCATATCT GCGAGTGGTG CGAAGTCTTC ACGTCTTGGA AGCCGGAGTT 
GCCAGTGGTT CACTTCACTC GACCAGCTTC GTGTATATCT TCGTCTTTGG CGGACTTATT 
TTCATCTTTA TGACCACCCT GTTTGTGTTC TATGCCATCC GAAAGATGAA ACATGAAAAG 
GTGTTGAAAC AACGCATCGA AACCGTTCAC CAGTGGACCA AGAAAGTGAT CATCTTCAAG 
CCCGAAGGTG GCGGAGACTC CAGTGGTTCC ATGGACACCA TGATTATGCC GGTGGTTAGG 
ATACAGAAAC AGCGCACCAC TGTTCTTCAG AATGGCAACG AGCCGGCTCC ATTCAATGAA 
TATGAATTTC CACTGGACTC GAACTGGGAA CTGCCCAGAA GTCATTTGGT ACTGGGTGCC 
ACTTTGGGAG AAGGTGCTTT CGGACGAGTG GTCATGGCGG AGGTCAATAA TGCCATTGTC 
GCCGTGAAAA TGGTGAAGGA AGGACACACG GATGATGACA TTGCCAGCTT GGTGCGGGAA 
ATGGAAGTGA TGAAGATCAT TGGGCGACAT ATCAATATTA TTAACTTACT TGGTTGCTGC 
AGTCAAAATG GTCCGCTCTA TGTGATTGTC GAGTATGCGC CACACGGAAA TCTCAAGGAC 
TTCCTCTATA AAAATCGACC CTTCGGAAGG GATCAGGATA GGGACAGCTC GCAACCGCCG 
CCATCGCCAC CAGCTCATGT GATAACCGAA AAGGATCTGA TCAAATTTGC CCACCAAATT 
GCCAGAGGAA TGGACTATTT GGCCTCGCGG CGATGCATCC ATCGAGATTT GGCAGCCAGG 
AATGTGCTCG TCAGCGATGA TTATGTGCTG AAGATTGCTG ATTTTGGACT GGCGAGGGAC 
ATTCAAAGCA CGGATTACTA TCGGAAGAAC ACAAATGGCA GGCTACCCAT CAAATGGATG 
GCACCGGAGT CGCTGCAGGA GAAATTCTAT GATTCCAAGA GCGATGTCTG GTCATATGGC 
ATCCTGCTGT GGGAGATCAT GACCTATGGG CAGCAACCAT ATCCAACTAT CATGTCCGCT 
GAGGAGCTGT ACACCTATCT CATGTCCGGT CAGCGGATGG AGAAACCAGC GAAATGCTCC 
ATGAACATCT ACATTCTGAT GCGACAATGT TGGCATTTCA ACGCCGACGA TCGGCCACCT 
TTTACGGAAA TTGTTGAGTA TATGGACAAG CTGCTCCAGA CGAAGGAGGA CTACCTCGAT 
GTGGATATCG CCAATCTGGA TACACCGCCC TCGACTAGCG ACGAGGAGGA AGATGAAACG 
GACAACCTGC AGAAGTGGTG TAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524394.2
CDS	1134..3323
Translation	MAAAWSWRASHSTITMTSGSLVVLFLLLSIWQPAVQVEGRRQMANSQEMIKDHLGARSQNKTPAITNNANQSSTSSADLDDGAADDDDNKADLPVNVSSKPYWRNPKKMSFLQTRPSGSLLTLNCHALGNPEPNITWYRNGTVDWTRGYGSLKRNRWTLTMEDLVPGDCGNYTCKVCNSLGCIRHDTQVIVSDRVNHKPILMTGPLNLTLVVNSTGSMHCKYLSDLTSKKAWIFVPCHGMTNCSNNRSIIAEDKDQLDFVNVRMEQEGWYTCVESNSLGQSNSTAYLRVVRSLHVLEAGVASGSLHSTSFVYIFVFGGLIFIFMTTLFVFYAIRKMKHEKVLKQRIETVHQWTKKVIIFKPEGGGDSSGSMDTMIMPVVRIQKQRTTVLQNGNEPAPFNEYEFPLDSNWELPRSHLVLGATLGEGAFGRVVMAEVNNAIVAVKMVKEGHTDDDIASLVREMEVMKIIGRHINIINLLGCCSQNGPLYVIVEYAPHGNLKDFLYKNRPFGRDQDRDSSQPPPSPPAHVITEKDLIKFAHQIARGMDYLASRRCIHRDLAARNVLVSDDYVLKIADFGLARDIQSTDYYRKNTNGRLPIKWMAPESLQEKFYDSKSDVWSYGILLWEIMTYGQQPYPTIMSAEELYTYLMSGQRMEKPAKCSMNIYILMRQCWHFNADDRPPFTEIVEYMDKLLQTKEDYLDVDIANLDTPPSTSDEEEDETDNLQKWCNY

Target ORF information:

RefSeq Version	NM_079670.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heartless (htl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079670.3

ORF Insert Sequence:

ATGGCTGCCG CCTGGAGTTG GCGAGCCAGT CACTCAACGA TCACAATGAC AAGCGGATCG 
CTGGTAGTGC TCTTCCTGTT GCTTTCGATT TGGCAGCCAG CCGTGCAGGT TGAAGGACGA 
CGCCAAATGG CCAATAGCCA GGAGATGATC AAGGATCATT TGGGAGCCAG GAGTCAAAAC 
AAGACACCAG CCATCACGAA CAATGCTAAT CAATCGAGCA CATCCAGTGC GGATTTGGAT 
GATGGCGCCG CAGATGATGA TGATAACAAG GCCGATTTGC CGGTGAATGT CAGTTCGAAA 
CCCTACTGGC GTAATCCCAA AAAGATGAGT TTTCTGCAGA CGCGACCCTC GGGCTCACTG 
CTCACCCTCA ATTGCCATGC CCTGGGCAAT CCTGAGCCCA ATATCACATG GTATCGCAAT 
GGCACCGTCG ACTGGACGCG CGGCTATGGG AGCCTGAAGC GGAATCGATG GACCCTGACG 
ATGGAGGATC TGGTGCCGGG CGATTGTGGC AACTACACCT GCAAGGTGTG CAATTCATTG 
GGTTGCATTC GTCACGACAC CCAGGTGATT GTAAGCGATC GAGTTAATCA CAAACCGATC 
CTGATGACCG GTCCATTGAA TCTCACACTG GTGGTCAATT CCACAGGCAG CATGCACTGC 
AAATATCTAT CGGACTTGAC CAGCAAGAAG GCGTGGATCT TTGTGCCCTG CCATGGCATG 
ACCAACTGCT CCAACAATCG ATCCATCATT GCCGAGGATA AGGATCAGTT GGACTTCGTG 
AACGTTAGGA TGGAGCAGGA GGGCTGGTAC ACGTGCGTCG AAAGCAACAG CCTGGGTCAA 
TCCAATAGCA CTGCATATCT GCGAGTGGTG CGAAGTCTTC ACGTCTTGGA AGCCGGAGTT 
GCCAGTGGTT CACTTCACTC GACCAGCTTC GTGTATATCT TCGTCTTTGG CGGACTTATT 
TTCATCTTTA TGACCACCCT GTTTGTGTTC TATGCCATCC GAAAGATGAA ACATGAAAAG 
GTGTTGAAAC AACGCATCGA AACCGTTCAC CAGTGGACCA AGAAAGTGAT CATCTTCAAG 
CCCGAAGGTG GCGGAGACTC CAGTGGTTCC ATGGACACCA TGATTATGCC GGTGGTTAGG 
ATACAGAAAC AGCGCACCAC TGTTCTTCAG AATGGCAACG AGCCGGCTCC ATTCAATGAA 
TATGAATTTC CACTGGACTC GAACTGGGAA CTGCCCAGAA GTCATTTGGT ACTGGGTGCC 
ACTTTGGGAG AAGGTGCTTT CGGACGAGTG GTCATGGCGG AGGTCAATAA TGCCATTGTC 
GCCGTGAAAA TGGTGAAGGA AGGACACACG GATGATGACA TTGCCAGCTT GGTGCGGGAA 
ATGGAAGTGA TGAAGATCAT TGGGCGACAT ATCAATATTA TTAACTTACT TGGTTGCTGC 
AGTCAAAATG GTCCGCTCTA TGTGATTGTC GAGTATGCGC CACACGGAAA TCTCAAGGAC 
TTCCTCTATA AAAATCGACC CTTCGGAAGG GATCAGGATA GGGACAGCTC GCAACCGCCG 
CCATCGCCAC CAGCTCATGT GATAACCGAA AAGGATCTGA TCAAATTTGC CCACCAAATT 
GCCAGAGGAA TGGACTATTT GGCCTCGCGG CGATGCATCC ATCGAGATTT GGCAGCCAGG 
AATGTGCTCG TCAGCGATGA TTATGTGCTG AAGATTGCTG ATTTTGGACT GGCGAGGGAC 
ATTCAAAGCA CGGATTACTA TCGGAAGAAC ACAAATGGCA GGCTACCCAT CAAATGGATG 
GCACCGGAGT CGCTGCAGGA GAAATTCTAT GATTCCAAGA GCGATGTCTG GTCATATGGC 
ATCCTGCTGT GGGAGATCAT GACCTATGGG CAGCAACCAT ATCCAACTAT CATGTCCGCT 
GAGGAGCTGT ACACCTATCT CATGTCCGGT CAGCGGATGG AGAAACCAGC GAAATGCTCC 
ATGAACATCT ACATTCTGAT GCGACAATGT TGGCATTTCA ACGCCGACGA TCGGCCACCT 
TTTACGGAAA TTGTTGAGTA TATGGACAAG CTGCTCCAGA CGAAGGAGGA CTACCTCGAT 
GTGGATATCG CCAATCTGGA TACACCGCCC TCGACTAGCG ACGAGGAGGA AGATGAAACG 
GACAACCTGC AGAAGTGGTG TAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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htl cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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