rec cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rec
Entrez Gene ID	49241
Full Name	recombination-defective
Synonyms	CG14875,CG31293,CG4828,Dmel\CG31293,MCM8,rec-1,rec1
General protein information	Preferred Names recombination-defective Names CG31293 gene product from transcript CG31293-RA CG31293-PA CG31293-PB mini-chromosome maintenance 8 rec-PA rec-PB recombination defective
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89A5-89A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_169674.2	NP_732072.1	recombination-defective, isoform A
NM_001275686.1	NP_001262615.1	recombination-defective, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Essential for DNA replication
Title: REC, Drosophila MCM8, drives formation of meiotic crossovers.

rec gene has no, or only a minor, role in DNA repair and recombination in somatic cells
Title: REC, a new member of the MCM-related protein family, is required for meiotic recombination in Drosophila.

The following rec gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rec cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06971	NM_169674.2 Latest version!	Drosophila melanogaster recombination-defective (rec), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06971	NM_001275686.1 Latest version!	Drosophila melanogaster recombination-defective (rec), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rec ( NM_169674.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732072.1 Drosophila melanogaster recombination-defective (rec), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06971
Clone ID Related Accession (Same CDS sequence)	NM_169674.2 , NM_001275686.1
Accession Version	NM_169674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	recombination-defective, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169674.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCCAA CGCTTTCCCG GGGGGGCGCC CCATCGCGAT TTGTGCGTGG TCGTGGTCGC 
GGAGGCGGAG CCTACCGTCC GTACTTCTAC TTTCGGCGCA ATGGACGTGT GATCCCAGCC 
GGAGGCAATC GACAACCCAA CCAAGGGGAA CCTGGAGCAC CAGACGCTCC TTCTGTTCCG 
CCTGCAACTC GGCAACCCAG GGGCTGGAGC CGCACAGCCG GCAAGCGAGG ACGCAATGCT 
CACTTGGACT GCAGCTTTCT GCGGCCGGAA AACTATGCCG CACCAGAAGA CGGGCTCCAG 
GTGCAGAGCA TTGCGGTGGA TAATCCACGG GCTTATTCCG GCTGGCGACT GTACTTCTTG 
CGCGAGAAAT ACGAGGAGGG CAACGAACTG GCCACAAGGA TAATGGCCGT GGAGGCATAC 
TACCAACGTA ATCCTCACAC CTTCGATTTT GTGATGATAC GGGATCGGGG ATTCTTCCCC 
CTCTGCGCAG CAGGCATCAA ATCAGATGCC CAGCTAAAGG AGGTTTGGCC TAGTTTGTCC 
GAAGACATCC GTGAGCACCC GCTTCGCACG CTAGGAACCC TGTCGTTGGC TATGCACACT 
GTGGTCGTCA ATCACCAGCT GGACTGTAAT GATTCCACCA CAGCTATGGT GTCCACTCCG 
GAACAGTACG TCCTTCCAAC CCCACGGGTA CGCAAGATCT ACGCACGACC GGATGATTTT 
GTGCCTGTGG AATCAATAGA AGGAATAAGC CATTCCCGAG TGGACACTTT GTTTTCCATC 
CGGGGTTTTG TGAGCAATGT GGGCGAGCCT AGCTACAGCC TCACGTGGCA AGCCTTTCGC 
TGCAGTCGCT GCCAGATGGA GATTGCCATG CGCCAACGGG GAACCTTCCA GCCACGTCCT 
TATCAATGCA AGCGGTCGGA GTGTGTGGCT CGTGACGAAT TCGTGCCTCT TCGCAGTTCC 
CCATACACCA GGCTTTCAAT CCGACAGATT ATCCGCGTGG AGGAGTCCAG TTTGAACTTG 
GTCCACGACT TTGAGACCAG CATGCCTGCT GAAATGGACG TGGAGCTGCG ACATGATCTA 
GTTGATGCTG TGCGTGTCGG ACAGGAGGTC GTGGTAACTG GCATCCTTAA GCTGCAGGAA 
TTGGGCGACG ATACCACCAC TGGAGACACC TCCAACCAGA TGCAGCCCTA TCTCAAGGCA 
GTCAGCATTC GCGATGCGAG CAGCATCAAG CGGGAGTTCA GCGAACGTGA CTTGGAAGCG 
ATTGTCATGA TCAATGCGGA ACCCAACAGC TTTAAGCTAC TGGTGCAATC CATTGCCCCG 
GAGGTATACG GACATGAGCT ACCCAAAGCC GCCTGTTTAC TATCCCTTCT CGGCGGGAAA 
GGTGCGGAGA CTGAAGCCAT CAACGTGCTC CTGGTGGGTG ATCCCGGAAT TGGAAAGACC 
AAGATTCTGC AAAGTTGTGC TCAGATCGCT GAACGTGGTG CCCACGTGAG TGGAAAACGT 
GGGGCTCAAT CCGCACAGCA ACTGGGTGTT ACGTTTGCTG GGCGTAACAA GCGTGTGCTA 
CAAGCGGGTT CCTTGATGAT GGCCAGTGGA GGCGGTCACT GCACCCTCGA TGATGTGGAT 
AAACTTGCCT CCAAGCAGGC CGTACTGTTG CAGTGCCTAC AATCAGAGGA AGTAAATCTT 
CCACTTGCAG GAGCATTTGC CTCATTCCCG GCGCAACCTT CGGTGATAGC GTGCGCAAAT 
CCACAAAGAG GACAATACGA TGAGGGGCGC TACCTTCTAC AGAATATTAA TATTTCACCC 
TCTTTGCTGC GGGAGTTCCA TTTGGTTTAC ATATTATTGG ACAAGCCCTC GGAGAGAGAT 
ATGTCGCTGA CTGCCCACGT AAGGGCTCTC CATGCGGGCG CTAGGAAACG AGCGAGAATT 
GCAGCCCGCT ATGCTCTTAA GCCCAAAATG AGCGACTCCA TGTGCGAGGT CAGCCTTAAT 
GTGCCGGCAG CGGGAAAAGA TGATGACACA ATCAAAACGG AGGACGATAA TGACAGCATT 
ATGCAGCAGG ATTATGATCT TGACAAACGT CTGGAGGTAC TTCCGGAGGA GGGGGACTTG 
GACCTGCTCC CACCCATACT GATTAAGAAG TTCTTGTCCT ATGCTCGCCA GGAATTAAAT 
CCGGTCCTAA ACGAGGACGC CAGCAATGCT GTTCTGCGTT ACTTCCTTGA GCTCAAAGGA 
AGCTGCAATC TCGATGAAGA CGTTTCCAGC CAGATTGGCG CAGGGCAACT TCTTGCGATA 
ATTCACCTGT CGCAGGCACG TGCTCGCCTG GATTTATCCC ACGTGGTCAG TCCGCAGCAC 
GTGCGCGATG TGATCGCCCT GCTGACCGAG AGCATCACCC AGACCAGCCT CAAGGAGGGC 
TCCAGCAGGC AAGGAACTCG AGGAGGAGGC GGCGCTGGAG GAGGAGCCGG AAAGAGCGCT 
CAACTGCGCA ATTTTTTGGA GCTTACGAAG AGACGTTCGG CGGCATTGGG GCGACGCATC 
TTCGAATTCG ATGAGCTAAA GGAAATTGGA ACCCGGGCTG GCATTTTGAC TGGCTTTAGC 
CAGCTGGTGG AAATGGCTAA CCTGGGTGGC TATTTGCTCA TGAAGGGCGC AAACATGTAC 
GAAGTGGTTC CGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732072.1
CDS	16..2673
Translation	MNPTLSRGGAPSRFVRGRGRGGGAYRPYFYFRRNGRVIPAGGNRQPNQGEPGAPDAPSVPPATRQPRGWSRTAGKRGRNAHLDCSFLRPENYAAPEDGLQVQSIAVDNPRAYSGWRLYFLREKYEEGNELATRIMAVEAYYQRNPHTFDFVMIRDRGFFPLCAAGIKSDAQLKEVWPSLSEDIREHPLRTLGTLSLAMHTVVVNHQLDCNDSTTAMVSTPEQYVLPTPRVRKIYARPDDFVPVESIEGISHSRVDTLFSIRGFVSNVGEPSYSLTWQAFRCSRCQMEIAMRQRGTFQPRPYQCKRSECVARDEFVPLRSSPYTRLSIRQIIRVEESSLNLVHDFETSMPAEMDVELRHDLVDAVRVGQEVVVTGILKLQELGDDTTTGDTSNQMQPYLKAVSIRDASSIKREFSERDLEAIVMINAEPNSFKLLVQSIAPEVYGHELPKAACLLSLLGGKGAETEAINVLLVGDPGIGKTKILQSCAQIAERGAHVSGKRGAQSAQQLGVTFAGRNKRVLQAGSLMMASGGGHCTLDDVDKLASKQAVLLQCLQSEEVNLPLAGAFASFPAQPSVIACANPQRGQYDEGRYLLQNINISPSLLREFHLVYILLDKPSERDMSLTAHVRALHAGARKRARIAARYALKPKMSDSMCEVSLNVPAAGKDDDTIKTEDDNDSIMQQDYDLDKRLEVLPEEGDLDLLPPILIKKFLSYARQELNPVLNEDASNAVLRYFLELKGSCNLDEDVSSQIGAGQLLAIIHLSQARARLDLSHVVSPQHVRDVIALLTESITQTSLKEGSSRQGTRGGGGAGGGAGKSAQLRNFLELTKRRSAALGRRIFEFDELKEIGTRAGILTGFSQLVEMANLGGYLLMKGANMYEVVPD

Target ORF information:

RefSeq Version	NM_169674.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster recombination-defective (rec), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169674.2

ORF Insert Sequence:

ATGAATCCAA CGCTTTCCCG GGGGGGCGCC CCATCGCGAT TTGTGCGTGG TCGTGGTCGC 
GGAGGCGGAG CCTACCGTCC GTACTTCTAC TTTCGGCGCA ATGGACGTGT GATCCCAGCC 
GGAGGCAATC GACAACCCAA CCAAGGGGAA CCTGGAGCAC CAGACGCTCC TTCTGTTCCG 
CCTGCAACTC GGCAACCCAG GGGCTGGAGC CGCACAGCCG GCAAGCGAGG ACGCAATGCT 
CACTTGGACT GCAGCTTTCT GCGGCCGGAA AACTATGCCG CACCAGAAGA CGGGCTCCAG 
GTGCAGAGCA TTGCGGTGGA TAATCCACGG GCTTATTCCG GCTGGCGACT GTACTTCTTG 
CGCGAGAAAT ACGAGGAGGG CAACGAACTG GCCACAAGGA TAATGGCCGT GGAGGCATAC 
TACCAACGTA ATCCTCACAC CTTCGATTTT GTGATGATAC GGGATCGGGG ATTCTTCCCC 
CTCTGCGCAG CAGGCATCAA ATCAGATGCC CAGCTAAAGG AGGTTTGGCC TAGTTTGTCC 
GAAGACATCC GTGAGCACCC GCTTCGCACG CTAGGAACCC TGTCGTTGGC TATGCACACT 
GTGGTCGTCA ATCACCAGCT GGACTGTAAT GATTCCACCA CAGCTATGGT GTCCACTCCG 
GAACAGTACG TCCTTCCAAC CCCACGGGTA CGCAAGATCT ACGCACGACC GGATGATTTT 
GTGCCTGTGG AATCAATAGA AGGAATAAGC CATTCCCGAG TGGACACTTT GTTTTCCATC 
CGGGGTTTTG TGAGCAATGT GGGCGAGCCT AGCTACAGCC TCACGTGGCA AGCCTTTCGC 
TGCAGTCGCT GCCAGATGGA GATTGCCATG CGCCAACGGG GAACCTTCCA GCCACGTCCT 
TATCAATGCA AGCGGTCGGA GTGTGTGGCT CGTGACGAAT TCGTGCCTCT TCGCAGTTCC 
CCATACACCA GGCTTTCAAT CCGACAGATT ATCCGCGTGG AGGAGTCCAG TTTGAACTTG 
GTCCACGACT TTGAGACCAG CATGCCTGCT GAAATGGACG TGGAGCTGCG ACATGATCTA 
GTTGATGCTG TGCGTGTCGG ACAGGAGGTC GTGGTAACTG GCATCCTTAA GCTGCAGGAA 
TTGGGCGACG ATACCACCAC TGGAGACACC TCCAACCAGA TGCAGCCCTA TCTCAAGGCA 
GTCAGCATTC GCGATGCGAG CAGCATCAAG CGGGAGTTCA GCGAACGTGA CTTGGAAGCG 
ATTGTCATGA TCAATGCGGA ACCCAACAGC TTTAAGCTAC TGGTGCAATC CATTGCCCCG 
GAGGTATACG GACATGAGCT ACCCAAAGCC GCCTGTTTAC TATCCCTTCT CGGCGGGAAA 
GGTGCGGAGA CTGAAGCCAT CAACGTGCTC CTGGTGGGTG ATCCCGGAAT TGGAAAGACC 
AAGATTCTGC AAAGTTGTGC TCAGATCGCT GAACGTGGTG CCCACGTGAG TGGAAAACGT 
GGGGCTCAAT CCGCACAGCA ACTGGGTGTT ACGTTTGCTG GGCGTAACAA GCGTGTGCTA 
CAAGCGGGTT CCTTGATGAT GGCCAGTGGA GGCGGTCACT GCACCCTCGA TGATGTGGAT 
AAACTTGCCT CCAAGCAGGC CGTACTGTTG CAGTGCCTAC AATCAGAGGA AGTAAATCTT 
CCACTTGCAG GAGCATTTGC CTCATTCCCG GCGCAACCTT CGGTGATAGC GTGCGCAAAT 
CCACAAAGAG GACAATACGA TGAGGGGCGC TACCTTCTAC AGAATATTAA TATTTCACCC 
TCTTTGCTGC GGGAGTTCCA TTTGGTTTAC ATATTATTGG ACAAGCCCTC GGAGAGAGAT 
ATGTCGCTGA CTGCCCACGT AAGGGCTCTC CATGCGGGCG CTAGGAAACG AGCGAGAATT 
GCAGCCCGCT ATGCTCTTAA GCCCAAAATG AGCGACTCCA TGTGCGAGGT CAGCCTTAAT 
GTGCCGGCAG CGGGAAAAGA TGATGACACA ATCAAAACGG AGGACGATAA TGACAGCATT 
ATGCAGCAGG ATTATGATCT TGACAAACGT CTGGAGGTAC TTCCGGAGGA GGGGGACTTG 
GACCTGCTCC CACCCATACT GATTAAGAAG TTCTTGTCCT ATGCTCGCCA GGAATTAAAT 
CCGGTCCTAA ACGAGGACGC CAGCAATGCT GTTCTGCGTT ACTTCCTTGA GCTCAAAGGA 
AGCTGCAATC TCGATGAAGA CGTTTCCAGC CAGATTGGCG CAGGGCAACT TCTTGCGATA 
ATTCACCTGT CGCAGGCACG TGCTCGCCTG GATTTATCCC ACGTGGTCAG TCCGCAGCAC 
GTGCGCGATG TGATCGCCCT GCTGACCGAG AGCATCACCC AGACCAGCCT CAAGGAGGGC 
TCCAGCAGGC AAGGAACTCG AGGAGGAGGC GGCGCTGGAG GAGGAGCCGG AAAGAGCGCT 
CAACTGCGCA ATTTTTTGGA GCTTACGAAG AGACGTTCGG CGGCATTGGG GCGACGCATC 
TTCGAATTCG ATGAGCTAAA GGAAATTGGA ACCCGGGCTG GCATTTTGAC TGGCTTTAGC 
CAGCTGGTGG AAATGGCTAA CCTGGGTGGC TATTTGCTCA TGAAGGGCGC AAACATGTAC 
GAAGTGGTTC CGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rec ( NM_001275686.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262615.1 Drosophila melanogaster recombination-defective (rec), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06971
Clone ID Related Accession (Same CDS sequence)	NM_169674.2 , NM_001275686.1
Accession Version	NM_001275686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	recombination-defective, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCCAA CGCTTTCCCG GGGGGGCGCC CCATCGCGAT TTGTGCGTGG TCGTGGTCGC 
GGAGGCGGAG CCTACCGTCC GTACTTCTAC TTTCGGCGCA ATGGACGTGT GATCCCAGCC 
GGAGGCAATC GACAACCCAA CCAAGGGGAA CCTGGAGCAC CAGACGCTCC TTCTGTTCCG 
CCTGCAACTC GGCAACCCAG GGGCTGGAGC CGCACAGCCG GCAAGCGAGG ACGCAATGCT 
CACTTGGACT GCAGCTTTCT GCGGCCGGAA AACTATGCCG CACCAGAAGA CGGGCTCCAG 
GTGCAGAGCA TTGCGGTGGA TAATCCACGG GCTTATTCCG GCTGGCGACT GTACTTCTTG 
CGCGAGAAAT ACGAGGAGGG CAACGAACTG GCCACAAGGA TAATGGCCGT GGAGGCATAC 
TACCAACGTA ATCCTCACAC CTTCGATTTT GTGATGATAC GGGATCGGGG ATTCTTCCCC 
CTCTGCGCAG CAGGCATCAA ATCAGATGCC CAGCTAAAGG AGGTTTGGCC TAGTTTGTCC 
GAAGACATCC GTGAGCACCC GCTTCGCACG CTAGGAACCC TGTCGTTGGC TATGCACACT 
GTGGTCGTCA ATCACCAGCT GGACTGTAAT GATTCCACCA CAGCTATGGT GTCCACTCCG 
GAACAGTACG TCCTTCCAAC CCCACGGGTA CGCAAGATCT ACGCACGACC GGATGATTTT 
GTGCCTGTGG AATCAATAGA AGGAATAAGC CATTCCCGAG TGGACACTTT GTTTTCCATC 
CGGGGTTTTG TGAGCAATGT GGGCGAGCCT AGCTACAGCC TCACGTGGCA AGCCTTTCGC 
TGCAGTCGCT GCCAGATGGA GATTGCCATG CGCCAACGGG GAACCTTCCA GCCACGTCCT 
TATCAATGCA AGCGGTCGGA GTGTGTGGCT CGTGACGAAT TCGTGCCTCT TCGCAGTTCC 
CCATACACCA GGCTTTCAAT CCGACAGATT ATCCGCGTGG AGGAGTCCAG TTTGAACTTG 
GTCCACGACT TTGAGACCAG CATGCCTGCT GAAATGGACG TGGAGCTGCG ACATGATCTA 
GTTGATGCTG TGCGTGTCGG ACAGGAGGTC GTGGTAACTG GCATCCTTAA GCTGCAGGAA 
TTGGGCGACG ATACCACCAC TGGAGACACC TCCAACCAGA TGCAGCCCTA TCTCAAGGCA 
GTCAGCATTC GCGATGCGAG CAGCATCAAG CGGGAGTTCA GCGAACGTGA CTTGGAAGCG 
ATTGTCATGA TCAATGCGGA ACCCAACAGC TTTAAGCTAC TGGTGCAATC CATTGCCCCG 
GAGGTATACG GACATGAGCT ACCCAAAGCC GCCTGTTTAC TATCCCTTCT CGGCGGGAAA 
GGTGCGGAGA CTGAAGCCAT CAACGTGCTC CTGGTGGGTG ATCCCGGAAT TGGAAAGACC 
AAGATTCTGC AAAGTTGTGC TCAGATCGCT GAACGTGGTG CCCACGTGAG TGGAAAACGT 
GGGGCTCAAT CCGCACAGCA ACTGGGTGTT ACGTTTGCTG GGCGTAACAA GCGTGTGCTA 
CAAGCGGGTT CCTTGATGAT GGCCAGTGGA GGCGGTCACT GCACCCTCGA TGATGTGGAT 
AAACTTGCCT CCAAGCAGGC CGTACTGTTG CAGTGCCTAC AATCAGAGGA AGTAAATCTT 
CCACTTGCAG GAGCATTTGC CTCATTCCCG GCGCAACCTT CGGTGATAGC GTGCGCAAAT 
CCACAAAGAG GACAATACGA TGAGGGGCGC TACCTTCTAC AGAATATTAA TATTTCACCC 
TCTTTGCTGC GGGAGTTCCA TTTGGTTTAC ATATTATTGG ACAAGCCCTC GGAGAGAGAT 
ATGTCGCTGA CTGCCCACGT AAGGGCTCTC CATGCGGGCG CTAGGAAACG AGCGAGAATT 
GCAGCCCGCT ATGCTCTTAA GCCCAAAATG AGCGACTCCA TGTGCGAGGT CAGCCTTAAT 
GTGCCGGCAG CGGGAAAAGA TGATGACACA ATCAAAACGG AGGACGATAA TGACAGCATT 
ATGCAGCAGG ATTATGATCT TGACAAACGT CTGGAGGTAC TTCCGGAGGA GGGGGACTTG 
GACCTGCTCC CACCCATACT GATTAAGAAG TTCTTGTCCT ATGCTCGCCA GGAATTAAAT 
CCGGTCCTAA ACGAGGACGC CAGCAATGCT GTTCTGCGTT ACTTCCTTGA GCTCAAAGGA 
AGCTGCAATC TCGATGAAGA CGTTTCCAGC CAGATTGGCG CAGGGCAACT TCTTGCGATA 
ATTCACCTGT CGCAGGCACG TGCTCGCCTG GATTTATCCC ACGTGGTCAG TCCGCAGCAC 
GTGCGCGATG TGATCGCCCT GCTGACCGAG AGCATCACCC AGACCAGCCT CAAGGAGGGC 
TCCAGCAGGC AAGGAACTCG AGGAGGAGGC GGCGCTGGAG GAGGAGCCGG AAAGAGCGCT 
CAACTGCGCA ATTTTTTGGA GCTTACGAAG AGACGTTCGG CGGCATTGGG GCGACGCATC 
TTCGAATTCG ATGAGCTAAA GGAAATTGGA ACCCGGGCTG GCATTTTGAC TGGCTTTAGC 
CAGCTGGTGG AAATGGCTAA CCTGGGTGGC TATTTGCTCA TGAAGGGCGC AAACATGTAC 
GAAGTGGTTC CGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262615.1
CDS	16..2673
Translation	MNPTLSRGGAPSRFVRGRGRGGGAYRPYFYFRRNGRVIPAGGNRQPNQGEPGAPDAPSVPPATRQPRGWSRTAGKRGRNAHLDCSFLRPENYAAPEDGLQVQSIAVDNPRAYSGWRLYFLREKYEEGNELATRIMAVEAYYQRNPHTFDFVMIRDRGFFPLCAAGIKSDAQLKEVWPSLSEDIREHPLRTLGTLSLAMHTVVVNHQLDCNDSTTAMVSTPEQYVLPTPRVRKIYARPDDFVPVESIEGISHSRVDTLFSIRGFVSNVGEPSYSLTWQAFRCSRCQMEIAMRQRGTFQPRPYQCKRSECVARDEFVPLRSSPYTRLSIRQIIRVEESSLNLVHDFETSMPAEMDVELRHDLVDAVRVGQEVVVTGILKLQELGDDTTTGDTSNQMQPYLKAVSIRDASSIKREFSERDLEAIVMINAEPNSFKLLVQSIAPEVYGHELPKAACLLSLLGGKGAETEAINVLLVGDPGIGKTKILQSCAQIAERGAHVSGKRGAQSAQQLGVTFAGRNKRVLQAGSLMMASGGGHCTLDDVDKLASKQAVLLQCLQSEEVNLPLAGAFASFPAQPSVIACANPQRGQYDEGRYLLQNINISPSLLREFHLVYILLDKPSERDMSLTAHVRALHAGARKRARIAARYALKPKMSDSMCEVSLNVPAAGKDDDTIKTEDDNDSIMQQDYDLDKRLEVLPEEGDLDLLPPILIKKFLSYARQELNPVLNEDASNAVLRYFLELKGSCNLDEDVSSQIGAGQLLAIIHLSQARARLDLSHVVSPQHVRDVIALLTESITQTSLKEGSSRQGTRGGGGAGGGAGKSAQLRNFLELTKRRSAALGRRIFEFDELKEIGTRAGILTGFSQLVEMANLGGYLLMKGANMYEVVPD

Target ORF information:

RefSeq Version	NM_001275686.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster recombination-defective (rec), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275686.1

ORF Insert Sequence:

ATGAATCCAA CGCTTTCCCG GGGGGGCGCC CCATCGCGAT TTGTGCGTGG TCGTGGTCGC 
GGAGGCGGAG CCTACCGTCC GTACTTCTAC TTTCGGCGCA ATGGACGTGT GATCCCAGCC 
GGAGGCAATC GACAACCCAA CCAAGGGGAA CCTGGAGCAC CAGACGCTCC TTCTGTTCCG 
CCTGCAACTC GGCAACCCAG GGGCTGGAGC CGCACAGCCG GCAAGCGAGG ACGCAATGCT 
CACTTGGACT GCAGCTTTCT GCGGCCGGAA AACTATGCCG CACCAGAAGA CGGGCTCCAG 
GTGCAGAGCA TTGCGGTGGA TAATCCACGG GCTTATTCCG GCTGGCGACT GTACTTCTTG 
CGCGAGAAAT ACGAGGAGGG CAACGAACTG GCCACAAGGA TAATGGCCGT GGAGGCATAC 
TACCAACGTA ATCCTCACAC CTTCGATTTT GTGATGATAC GGGATCGGGG ATTCTTCCCC 
CTCTGCGCAG CAGGCATCAA ATCAGATGCC CAGCTAAAGG AGGTTTGGCC TAGTTTGTCC 
GAAGACATCC GTGAGCACCC GCTTCGCACG CTAGGAACCC TGTCGTTGGC TATGCACACT 
GTGGTCGTCA ATCACCAGCT GGACTGTAAT GATTCCACCA CAGCTATGGT GTCCACTCCG 
GAACAGTACG TCCTTCCAAC CCCACGGGTA CGCAAGATCT ACGCACGACC GGATGATTTT 
GTGCCTGTGG AATCAATAGA AGGAATAAGC CATTCCCGAG TGGACACTTT GTTTTCCATC 
CGGGGTTTTG TGAGCAATGT GGGCGAGCCT AGCTACAGCC TCACGTGGCA AGCCTTTCGC 
TGCAGTCGCT GCCAGATGGA GATTGCCATG CGCCAACGGG GAACCTTCCA GCCACGTCCT 
TATCAATGCA AGCGGTCGGA GTGTGTGGCT CGTGACGAAT TCGTGCCTCT TCGCAGTTCC 
CCATACACCA GGCTTTCAAT CCGACAGATT ATCCGCGTGG AGGAGTCCAG TTTGAACTTG 
GTCCACGACT TTGAGACCAG CATGCCTGCT GAAATGGACG TGGAGCTGCG ACATGATCTA 
GTTGATGCTG TGCGTGTCGG ACAGGAGGTC GTGGTAACTG GCATCCTTAA GCTGCAGGAA 
TTGGGCGACG ATACCACCAC TGGAGACACC TCCAACCAGA TGCAGCCCTA TCTCAAGGCA 
GTCAGCATTC GCGATGCGAG CAGCATCAAG CGGGAGTTCA GCGAACGTGA CTTGGAAGCG 
ATTGTCATGA TCAATGCGGA ACCCAACAGC TTTAAGCTAC TGGTGCAATC CATTGCCCCG 
GAGGTATACG GACATGAGCT ACCCAAAGCC GCCTGTTTAC TATCCCTTCT CGGCGGGAAA 
GGTGCGGAGA CTGAAGCCAT CAACGTGCTC CTGGTGGGTG ATCCCGGAAT TGGAAAGACC 
AAGATTCTGC AAAGTTGTGC TCAGATCGCT GAACGTGGTG CCCACGTGAG TGGAAAACGT 
GGGGCTCAAT CCGCACAGCA ACTGGGTGTT ACGTTTGCTG GGCGTAACAA GCGTGTGCTA 
CAAGCGGGTT CCTTGATGAT GGCCAGTGGA GGCGGTCACT GCACCCTCGA TGATGTGGAT 
AAACTTGCCT CCAAGCAGGC CGTACTGTTG CAGTGCCTAC AATCAGAGGA AGTAAATCTT 
CCACTTGCAG GAGCATTTGC CTCATTCCCG GCGCAACCTT CGGTGATAGC GTGCGCAAAT 
CCACAAAGAG GACAATACGA TGAGGGGCGC TACCTTCTAC AGAATATTAA TATTTCACCC 
TCTTTGCTGC GGGAGTTCCA TTTGGTTTAC ATATTATTGG ACAAGCCCTC GGAGAGAGAT 
ATGTCGCTGA CTGCCCACGT AAGGGCTCTC CATGCGGGCG CTAGGAAACG AGCGAGAATT 
GCAGCCCGCT ATGCTCTTAA GCCCAAAATG AGCGACTCCA TGTGCGAGGT CAGCCTTAAT 
GTGCCGGCAG CGGGAAAAGA TGATGACACA ATCAAAACGG AGGACGATAA TGACAGCATT 
ATGCAGCAGG ATTATGATCT TGACAAACGT CTGGAGGTAC TTCCGGAGGA GGGGGACTTG 
GACCTGCTCC CACCCATACT GATTAAGAAG TTCTTGTCCT ATGCTCGCCA GGAATTAAAT 
CCGGTCCTAA ACGAGGACGC CAGCAATGCT GTTCTGCGTT ACTTCCTTGA GCTCAAAGGA 
AGCTGCAATC TCGATGAAGA CGTTTCCAGC CAGATTGGCG CAGGGCAACT TCTTGCGATA 
ATTCACCTGT CGCAGGCACG TGCTCGCCTG GATTTATCCC ACGTGGTCAG TCCGCAGCAC 
GTGCGCGATG TGATCGCCCT GCTGACCGAG AGCATCACCC AGACCAGCCT CAAGGAGGGC 
TCCAGCAGGC AAGGAACTCG AGGAGGAGGC GGCGCTGGAG GAGGAGCCGG AAAGAGCGCT 
CAACTGCGCA ATTTTTTGGA GCTTACGAAG AGACGTTCGG CGGCATTGGG GCGACGCATC 
TTCGAATTCG ATGAGCTAAA GGAAATTGGA ACCCGGGCTG GCATTTTGAC TGGCTTTAGC 
CAGCTGGTGG AAATGGCTAA CCTGGGTGGC TATTTGCTCA TGAAGGGCGC AAACATGTAC 
GAAGTGGTTC CGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rec cDNA ORF clone, Drosophila melanogaster(fruit fly)

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