APPL1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Gene Symbol	APPL1
Entrez Gene ID	416002
Full Name	adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
General protein information	Preferred Names adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 Names DCC-interacting protein 13-alpha adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004944584.3	XP_004944641.2	DCC-interacting protein 13-alpha
XM_414344.5	XP_414344.5	DCC-interacting protein 13-alpha
XM_004944584.2	XP_004944641.2	DCC-interacting protein 13-alpha
XM_414344.6	XP_414344.5	DCC-interacting protein 13-alpha

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	APPL1	XP_001173707.1
Canis lupus familiaris (dog)	APPL1	XP_849075.1
Gallus gallus (chicken)	APPL1	XP_414344.4
Bos taurus (cattle)	APPL1	NP_001092473.1
Mus musculus (house mouse)	Appl1	NP_660256.1
Homo sapiens (human)	APPL1	NP_036228.1
Rattus norvegicus (Norway rat)	Appl1	XP_006252792.1
Danio rerio (zebrafish)	appl1	NP_001121546.1
Xenopus tropicalis (tropical clawed frog)	appl1	XP_004914231.1

Gene Ontology

The following APPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa41640	XM_004944584.3 Latest version!	Gallus gallus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa41640	XM_414344.6 Latest version!	Gallus gallus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OGa41640	XM_004944584.2	Gallus gallus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OGa41640	XM_414344.5	Gallus gallus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APPL1 ( XM_004944584.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004944641.2 Gallus gallus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa41640
Clone ID Related Accession (Same CDS sequence)	XM_004944584.3 , XM_414344.5 , XM_004944584.2 , XM_414344.6
Accession Version	XM_004944584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	DCC-interacting protein 13-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004944584.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGCA TCGACAAGCT GCCCATCGAG GAGACGCTGG AAGACAGCCC CCAGACCCGT 
TCTTTGCTGG GAGTATTTGA AGAAGATGCT GCTGCAATTT CCAACTACGT CAATCAGTTA 
TTTCAAGCAA TGCATAGAGT ATATGATGCA CAGAATGAAT TAAGTGCAGC GACTCATCTG 
ACTTCAAAGC TTCTGAAGGA ATATGAGAAA CAGCACTTTC CACTAGGGGG AGATGATGAA 
ATTATGAGTT CTACTTTACA GCAATTTGCA AAAGTGATCG ACGAGCTCAG TTCTTGCCAT 
GCAGTACTTT CAACTCAACT TGCAGATGCA ATGATGTTTC CTATTACTCA GTTTAAAGAA 
AGAGATCTAA AAGAGATATT AACTCTAAAA GAAGTTTTCC AAATTGCAAG CAATGACCAC 
GACGCTGCCA TTACCCGATA CAGTCGGTTG TCAAAAAGAA GGGAAAATGA GAAGATCAAG 
GCTGAAGTTA CAGAAGATGT GTATACTTCC AGGAAAAAAC AGCATCAGAC TATGATGCAT 
TATTTCTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TTGCTGTGTT AGAACCCTTG 
CTAGGATACA TGCAAGCTCA GATAAGCTTT TTTAAAATGG GTTCAGAAAA TCTCACTGAG 
CAACTGGAGG AATTTCTCAC CAACATTGGC ACAAGTGTAC AAAATGTTCG CAGGGAAATG 
GAGTGCGAAA TAGAGAACAT GCAGCAAACT ATAGAAGACT TGGAAGTAGC CAGTGATCCA 
CTTTATTTGC CTGATCCGGA TCCTACGAAA TTTCCTGTCC ATAGAAATCT TACACGGAAA 
GCTGGCTATC TCAATGCAAG AAATAAAACA GGGCTGGTAT CTTCCAGTTG GGAGAGACAG 
TTCTACTTCA CTCAAGGTGG AAATCTGATG AGCCAGGCAA GAGGTGATGT GGCTGGGGGA 
CTTGTCATGG ACATAGACAA TTGTTCAGTA ATGGCTGTGG ACTGCGAAGA CAGACGGTAC 
TGTTTTCAAA TAACATCTTT TGATGGTAAA AAGTCTTCAA TCTTACAGGC AGAGAGTAAA 
AAAGATTATG AAGAGTGGAT ATGCACAATA AACAACATAT CTAAACAGAT ATATCTAAGT 
GAAAACCCTG AGGAAATTGC TGCACGTGTA AACCAGTCAG CTCTCGAGGC CGTTACTCCA 
TCACCTTCAT TTCAGCAGAG GCATGAGAGC ATGCGTCCGA CTGTACAATC TCGGCCTCCC 
ACAGCCCGCA CGAGCAGCAC GGGGTCACTG GGATCCGAAT CCGCCGCTCT GTCTGCACTG 
TCCTTGGATT CACTCGTTGC CCCTGACACT CCTATTCAAT TTGACATAAT ATCTCCAGTT 
AGTGAAGATC TGCCTGGTCA AGCAAAGTCT TCAGGGCAGT CAGGCAGACG CACAAATCCT 
TTTGGTGAAA CGGGAGGCTC AAAATCTGAA ACTGAAGATT CAATTCTTCA TCAGTTGTTC 
ATTGTAAGGT TTCTTGGCTC GATGGAGGTG AAGTCTGATG AAAGTCCAGA TGTTGTTTAT 
GAAACAATGC GTCAAATCCT AGCAGCTCGG GCCATCCATA ACATTTTCAG GATGACAGAA 
TCACATTTAT TAGTCACTTG TGACTGTTTA AAGTTAATTG ATCCACAGAC ACAAGTTACA 
AGACTACGAT TTCCTTTGCC CAATGTAGTC TTGTATGCTA CACATCAGGA GAATAAGCGC 
CTCTTTGGAT TTGTGCTCAG AACTTCAGGA GGGAGAGCTG ACAGTCGACA AACTTCCATC 
TGCTATATCT TTGAATCAAA CAATGAAGGG GAAAAGATTT GTGACTCCGT TGGACTGGCA 
AAACAGATTG CTTTCCATGC AGAACTGGAT CGAAAAGCAT CAGAAAAGCA AAAAGAAATG 
GAGAGAGTCA AAGAGAAACA ACAAAAAGAA CTGACTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAGGAGC AAAGCCGACT GATAGCTGCT TCCAACAGAT CAAACCCTAG CAGTGGAGAA 
GGACAGTTTG TCGTCCTTAG CAGCAGCCAG TCAGAAGACA GCGATTTAGG AGAGGATGGA 
AAGAAGAAAA GGGAATCTGA AACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004944641.2
CDS	88..2214
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDAAAISNYVNQLFQAMHRVYDAQNELSAATHLTSKLLKEYEKQHFPLGGDDEIMSSTLQQFAKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAITRYSRLSKRRENEKIKAEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIAVLEPLLGYMQAQISFFKMGSENLTEQLEEFLTNIGTSVQNVRREMECEIENMQQTIEDLEVASDPLYLPDPDPTKFPVHRNLTRKAGYLNARNKTGLVSSSWERQFYFTQGGNLMSQARGDVAGGLVMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDYEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESMRPTVQSRPPTARTSSTGSLGSESAALSALSLDSLVAPDTPIQFDIISPVSEDLPGQAKSSGQSGRRTNPFGETGGSKSETEDSILHQLFIVRFLGSMEVKSDESPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLRFPLPNVVLYATHQENKRLFGFVLRTSGGRADSRQTSICYIFESNNEGEKICDSVGLAKQIAFHAELDRKASEKQKEMERVKEKQQKELTKQKQIEKDLEEQSRLIAASNRSNPSSGEGQFVVLSSSQSEDSDLGEDGKKKRESET

Target ORF information:

RefSeq Version	XM_004944584.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004944584.3

ORF Insert Sequence:

ATGCCCGGCA TCGACAAGCT GCCCATCGAG GAGACGCTGG AAGACAGCCC CCAGACCCGT 
TCTTTGCTGG GAGTATTTGA AGAAGATGCT GCTGCAATTT CCAACTACGT CAATCAGTTA 
TTTCAAGCAA TGCATAGAGT ATATGATGCA CAGAATGAAT TAAGTGCAGC GACTCATCTG 
ACTTCAAAGC TTCTGAAGGA ATATGAGAAA CAGCACTTTC CACTAGGGGG AGATGATGAA 
ATTATGAGTT CTACTTTACA GCAATTTGCA AAAGTGATCG ACGAGCTCAG TTCTTGCCAT 
GCAGTACTTT CAACTCAACT TGCAGATGCA ATGATGTTTC CTATTACTCA GTTTAAAGAA 
AGAGATCTAA AAGAGATATT AACTCTAAAA GAAGTTTTCC AAATTGCAAG CAATGACCAC 
GACGCTGCCA TTACCCGATA CAGTCGGTTG TCAAAAAGAA GGGAAAATGA GAAGATCAAG 
GCTGAAGTTA CAGAAGATGT GTATACTTCC AGGAAAAAAC AGCATCAGAC TATGATGCAT 
TATTTCTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TTGCTGTGTT AGAACCCTTG 
CTAGGATACA TGCAAGCTCA GATAAGCTTT TTTAAAATGG GTTCAGAAAA TCTCACTGAG 
CAACTGGAGG AATTTCTCAC CAACATTGGC ACAAGTGTAC AAAATGTTCG CAGGGAAATG 
GAGTGCGAAA TAGAGAACAT GCAGCAAACT ATAGAAGACT TGGAAGTAGC CAGTGATCCA 
CTTTATTTGC CTGATCCGGA TCCTACGAAA TTTCCTGTCC ATAGAAATCT TACACGGAAA 
GCTGGCTATC TCAATGCAAG AAATAAAACA GGGCTGGTAT CTTCCAGTTG GGAGAGACAG 
TTCTACTTCA CTCAAGGTGG AAATCTGATG AGCCAGGCAA GAGGTGATGT GGCTGGGGGA 
CTTGTCATGG ACATAGACAA TTGTTCAGTA ATGGCTGTGG ACTGCGAAGA CAGACGGTAC 
TGTTTTCAAA TAACATCTTT TGATGGTAAA AAGTCTTCAA TCTTACAGGC AGAGAGTAAA 
AAAGATTATG AAGAGTGGAT ATGCACAATA AACAACATAT CTAAACAGAT ATATCTAAGT 
GAAAACCCTG AGGAAATTGC TGCACGTGTA AACCAGTCAG CTCTCGAGGC CGTTACTCCA 
TCACCTTCAT TTCAGCAGAG GCATGAGAGC ATGCGTCCGA CTGTACAATC TCGGCCTCCC 
ACAGCCCGCA CGAGCAGCAC GGGGTCACTG GGATCCGAAT CCGCCGCTCT GTCTGCACTG 
TCCTTGGATT CACTCGTTGC CCCTGACACT CCTATTCAAT TTGACATAAT ATCTCCAGTT 
AGTGAAGATC TGCCTGGTCA AGCAAAGTCT TCAGGGCAGT CAGGCAGACG CACAAATCCT 
TTTGGTGAAA CGGGAGGCTC AAAATCTGAA ACTGAAGATT CAATTCTTCA TCAGTTGTTC 
ATTGTAAGGT TTCTTGGCTC GATGGAGGTG AAGTCTGATG AAAGTCCAGA TGTTGTTTAT 
GAAACAATGC GTCAAATCCT AGCAGCTCGG GCCATCCATA ACATTTTCAG GATGACAGAA 
TCACATTTAT TAGTCACTTG TGACTGTTTA AAGTTAATTG ATCCACAGAC ACAAGTTACA 
AGACTACGAT TTCCTTTGCC CAATGTAGTC TTGTATGCTA CACATCAGGA GAATAAGCGC 
CTCTTTGGAT TTGTGCTCAG AACTTCAGGA GGGAGAGCTG ACAGTCGACA AACTTCCATC 
TGCTATATCT TTGAATCAAA CAATGAAGGG GAAAAGATTT GTGACTCCGT TGGACTGGCA 
AAACAGATTG CTTTCCATGC AGAACTGGAT CGAAAAGCAT CAGAAAAGCA AAAAGAAATG 
GAGAGAGTCA AAGAGAAACA ACAAAAAGAA CTGACTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAGGAGC AAAGCCGACT GATAGCTGCT TCCAACAGAT CAAACCCTAG CAGTGGAGAA 
GGACAGTTTG TCGTCCTTAG CAGCAGCCAG TCAGAAGACA GCGATTTAGG AGAGGATGGA 
AAGAAGAAAA GGGAATCTGA AACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_414344.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_414344.5 Gallus gallus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa41640
Clone ID Related Accession (Same CDS sequence)	XM_004944584.3 , XM_414344.5 , XM_004944584.2 , XM_414344.6
Accession Version	XM_414344.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	DCC-interacting protein 13-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_414344.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGCA TCGACAAGCT GCCCATCGAG GAGACGCTGG AAGACAGCCC CCAGACCCGT 
TCTTTGCTGG GAGTATTTGA AGAAGATGCT GCTGCAATTT CCAACTACGT CAATCAGTTA 
TTTCAAGCAA TGCATAGAGT ATATGATGCA CAGAATGAAT TAAGTGCAGC GACTCATCTG 
ACTTCAAAGC TTCTGAAGGA ATATGAGAAA CAGCACTTTC CACTAGGGGG AGATGATGAA 
ATTATGAGTT CTACTTTACA GCAATTTGCA AAAGTGATCG ACGAGCTCAG TTCTTGCCAT 
GCAGTACTTT CAACTCAACT TGCAGATGCA ATGATGTTTC CTATTACTCA GTTTAAAGAA 
AGAGATCTAA AAGAGATATT AACTCTAAAA GAAGTTTTCC AAATTGCAAG CAATGACCAC 
GACGCTGCCA TTACCCGATA CAGTCGGTTG TCAAAAAGAA GGGAAAATGA GAAGATCAAG 
GCTGAAGTTA CAGAAGATGT GTATACTTCC AGGAAAAAAC AGCATCAGAC TATGATGCAT 
TATTTCTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TTGCTGTGTT AGAACCCTTG 
CTAGGATACA TGCAAGCTCA GATAAGCTTT TTTAAAATGG GTTCAGAAAA TCTCACTGAG 
CAACTGGAGG AATTTCTCAC CAACATTGGC ACAAGTGTAC AAAATGTTCG CAGGGAAATG 
GAGTGCGAAA TAGAGAACAT GCAGCAAACT ATAGAAGACT TGGAAGTAGC CAGTGATCCA 
CTTTATTTGC CTGATCCGGA TCCTACGAAA TTTCCTGTCC ATAGAAATCT TACACGGAAA 
GCTGGCTATC TCAATGCAAG AAATAAAACA GGGCTGGTAT CTTCCAGTTG GGAGAGACAG 
TTCTACTTCA CTCAAGGTGG AAATCTGATG AGCCAGGCAA GAGGTGATGT GGCTGGGGGA 
CTTGTCATGG ACATAGACAA TTGTTCAGTA ATGGCTGTGG ACTGCGAAGA CAGACGGTAC 
TGTTTTCAAA TAACATCTTT TGATGGTAAA AAGTCTTCAA TCTTACAGGC AGAGAGTAAA 
AAAGATTATG AAGAGTGGAT ATGCACAATA AACAACATAT CTAAACAGAT ATATCTAAGT 
GAAAACCCTG AGGAAATTGC TGCACGTGTA AACCAGTCAG CTCTCGAGGC CGTTACTCCA 
TCACCTTCAT TTCAGCAGAG GCATGAGAGC ATGCGTCCGA CTGTACAATC TCGGCCTCCC 
ACAGCCCGCA CGAGCAGCAC GGGGTCACTG GGATCCGAAT CCGCCGCTCT GTCTGCACTG 
TCCTTGGATT CACTCGTTGC CCCTGACACT CCTATTCAAT TTGACATAAT ATCTCCAGTT 
AGTGAAGATC TGCCTGGTCA AGCAAAGTCT TCAGGGCAGT CAGGCAGACG CACAAATCCT 
TTTGGTGAAA CGGGAGGCTC AAAATCTGAA ACTGAAGATT CAATTCTTCA TCAGTTGTTC 
ATTGTAAGGT TTCTTGGCTC GATGGAGGTG AAGTCTGATG AAAGTCCAGA TGTTGTTTAT 
GAAACAATGC GTCAAATCCT AGCAGCTCGG GCCATCCATA ACATTTTCAG GATGACAGAA 
TCACATTTAT TAGTCACTTG TGACTGTTTA AAGTTAATTG ATCCACAGAC ACAAGTTACA 
AGACTACGAT TTCCTTTGCC CAATGTAGTC TTGTATGCTA CACATCAGGA GAATAAGCGC 
CTCTTTGGAT TTGTGCTCAG AACTTCAGGA GGGAGAGCTG ACAGTCGACA AACTTCCATC 
TGCTATATCT TTGAATCAAA CAATGAAGGG GAAAAGATTT GTGACTCCGT TGGACTGGCA 
AAACAGATTG CTTTCCATGC AGAACTGGAT CGAAAAGCAT CAGAAAAGCA AAAAGAAATG 
GAGAGAGTCA AAGAGAAACA ACAAAAAGAA CTGACTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAGGAGC AAAGCCGACT GATAGCTGCT TCCAACAGAT CAAACCCTAG CAGTGGAGAA 
GGACAGTTTG TCGTCCTTAG CAGCAGCCAG TCAGAAGACA GCGATTTAGG AGAGGATGGA 
AAGAAGAAAA GGGAATCTGA AACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_414344.5
CDS	126..2252
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDAAAISNYVNQLFQAMHRVYDAQNELSAATHLTSKLLKEYEKQHFPLGGDDEIMSSTLQQFAKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAITRYSRLSKRRENEKIKAEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIAVLEPLLGYMQAQISFFKMGSENLTEQLEEFLTNIGTSVQNVRREMECEIENMQQTIEDLEVASDPLYLPDPDPTKFPVHRNLTRKAGYLNARNKTGLVSSSWERQFYFTQGGNLMSQARGDVAGGLVMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDYEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESMRPTVQSRPPTARTSSTGSLGSESAALSALSLDSLVAPDTPIQFDIISPVSEDLPGQAKSSGQSGRRTNPFGETGGSKSETEDSILHQLFIVRFLGSMEVKSDESPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLRFPLPNVVLYATHQENKRLFGFVLRTSGGRADSRQTSICYIFESNNEGEKICDSVGLAKQIAFHAELDRKASEKQKEMERVKEKQQKELTKQKQIEKDLEEQSRLIAASNRSNPSSGEGQFVVLSSSQSEDSDLGEDGKKKRESET

Target ORF information:

RefSeq Version	XM_414344.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_414344.5

ORF Insert Sequence:

ATGCCCGGCA TCGACAAGCT GCCCATCGAG GAGACGCTGG AAGACAGCCC CCAGACCCGT 
TCTTTGCTGG GAGTATTTGA AGAAGATGCT GCTGCAATTT CCAACTACGT CAATCAGTTA 
TTTCAAGCAA TGCATAGAGT ATATGATGCA CAGAATGAAT TAAGTGCAGC GACTCATCTG 
ACTTCAAAGC TTCTGAAGGA ATATGAGAAA CAGCACTTTC CACTAGGGGG AGATGATGAA 
ATTATGAGTT CTACTTTACA GCAATTTGCA AAAGTGATCG ACGAGCTCAG TTCTTGCCAT 
GCAGTACTTT CAACTCAACT TGCAGATGCA ATGATGTTTC CTATTACTCA GTTTAAAGAA 
AGAGATCTAA AAGAGATATT AACTCTAAAA GAAGTTTTCC AAATTGCAAG CAATGACCAC 
GACGCTGCCA TTACCCGATA CAGTCGGTTG TCAAAAAGAA GGGAAAATGA GAAGATCAAG 
GCTGAAGTTA CAGAAGATGT GTATACTTCC AGGAAAAAAC AGCATCAGAC TATGATGCAT 
TATTTCTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TTGCTGTGTT AGAACCCTTG 
CTAGGATACA TGCAAGCTCA GATAAGCTTT TTTAAAATGG GTTCAGAAAA TCTCACTGAG 
CAACTGGAGG AATTTCTCAC CAACATTGGC ACAAGTGTAC AAAATGTTCG CAGGGAAATG 
GAGTGCGAAA TAGAGAACAT GCAGCAAACT ATAGAAGACT TGGAAGTAGC CAGTGATCCA 
CTTTATTTGC CTGATCCGGA TCCTACGAAA TTTCCTGTCC ATAGAAATCT TACACGGAAA 
GCTGGCTATC TCAATGCAAG AAATAAAACA GGGCTGGTAT CTTCCAGTTG GGAGAGACAG 
TTCTACTTCA CTCAAGGTGG AAATCTGATG AGCCAGGCAA GAGGTGATGT GGCTGGGGGA 
CTTGTCATGG ACATAGACAA TTGTTCAGTA ATGGCTGTGG ACTGCGAAGA CAGACGGTAC 
TGTTTTCAAA TAACATCTTT TGATGGTAAA AAGTCTTCAA TCTTACAGGC AGAGAGTAAA 
AAAGATTATG AAGAGTGGAT ATGCACAATA AACAACATAT CTAAACAGAT ATATCTAAGT 
GAAAACCCTG AGGAAATTGC TGCACGTGTA AACCAGTCAG CTCTCGAGGC CGTTACTCCA 
TCACCTTCAT TTCAGCAGAG GCATGAGAGC ATGCGTCCGA CTGTACAATC TCGGCCTCCC 
ACAGCCCGCA CGAGCAGCAC GGGGTCACTG GGATCCGAAT CCGCCGCTCT GTCTGCACTG 
TCCTTGGATT CACTCGTTGC CCCTGACACT CCTATTCAAT TTGACATAAT ATCTCCAGTT 
AGTGAAGATC TGCCTGGTCA AGCAAAGTCT TCAGGGCAGT CAGGCAGACG CACAAATCCT 
TTTGGTGAAA CGGGAGGCTC AAAATCTGAA ACTGAAGATT CAATTCTTCA TCAGTTGTTC 
ATTGTAAGGT TTCTTGGCTC GATGGAGGTG AAGTCTGATG AAAGTCCAGA TGTTGTTTAT 
GAAACAATGC GTCAAATCCT AGCAGCTCGG GCCATCCATA ACATTTTCAG GATGACAGAA 
TCACATTTAT TAGTCACTTG TGACTGTTTA AAGTTAATTG ATCCACAGAC ACAAGTTACA 
AGACTACGAT TTCCTTTGCC CAATGTAGTC TTGTATGCTA CACATCAGGA GAATAAGCGC 
CTCTTTGGAT TTGTGCTCAG AACTTCAGGA GGGAGAGCTG ACAGTCGACA AACTTCCATC 
TGCTATATCT TTGAATCAAA CAATGAAGGG GAAAAGATTT GTGACTCCGT TGGACTGGCA 
AAACAGATTG CTTTCCATGC AGAACTGGAT CGAAAAGCAT CAGAAAAGCA AAAAGAAATG 
GAGAGAGTCA AAGAGAAACA ACAAAAAGAA CTGACTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAGGAGC AAAGCCGACT GATAGCTGCT TCCAACAGAT CAAACCCTAG CAGTGGAGAA 
GGACAGTTTG TCGTCCTTAG CAGCAGCCAG TCAGAAGACA GCGATTTAGG AGAGGATGGA 
AAGAAGAAAA GGGAATCTGA AACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_004944584.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004944641.2 Gallus gallus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa41640
Clone ID Related Accession (Same CDS sequence)	XM_004944584.3 , XM_414344.5 , XM_004944584.2 , XM_414344.6
Accession Version	XM_004944584.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	DCC-interacting protein 13-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004944584.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGCA TCGACAAGCT GCCCATCGAG GAGACGCTGG AAGACAGCCC CCAGACCCGT 
TCTTTGCTGG GAGTATTTGA AGAAGATGCT GCTGCAATTT CCAACTACGT CAATCAGTTA 
TTTCAAGCAA TGCATAGAGT ATATGATGCA CAGAATGAAT TAAGTGCAGC GACTCATCTG 
ACTTCAAAGC TTCTGAAGGA ATATGAGAAA CAGCACTTTC CACTAGGGGG AGATGATGAA 
ATTATGAGTT CTACTTTACA GCAATTTGCA AAAGTGATCG ACGAGCTCAG TTCTTGCCAT 
GCAGTACTTT CAACTCAACT TGCAGATGCA ATGATGTTTC CTATTACTCA GTTTAAAGAA 
AGAGATCTAA AAGAGATATT AACTCTAAAA GAAGTTTTCC AAATTGCAAG CAATGACCAC 
GACGCTGCCA TTACCCGATA CAGTCGGTTG TCAAAAAGAA GGGAAAATGA GAAGATCAAG 
GCTGAAGTTA CAGAAGATGT GTATACTTCC AGGAAAAAAC AGCATCAGAC TATGATGCAT 
TATTTCTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TTGCTGTGTT AGAACCCTTG 
CTAGGATACA TGCAAGCTCA GATAAGCTTT TTTAAAATGG GTTCAGAAAA TCTCACTGAG 
CAACTGGAGG AATTTCTCAC CAACATTGGC ACAAGTGTAC AAAATGTTCG CAGGGAAATG 
GAGTGCGAAA TAGAGAACAT GCAGCAAACT ATAGAAGACT TGGAAGTAGC CAGTGATCCA 
CTTTATTTGC CTGATCCGGA TCCTACGAAA TTTCCTGTCC ATAGAAATCT TACACGGAAA 
GCTGGCTATC TCAATGCAAG AAATAAAACA GGGCTGGTAT CTTCCAGTTG GGAGAGACAG 
TTCTACTTCA CTCAAGGTGG AAATCTGATG AGCCAGGCAA GAGGTGATGT GGCTGGGGGA 
CTTGTCATGG ACATAGACAA TTGTTCAGTA ATGGCTGTGG ACTGCGAAGA CAGACGGTAC 
TGTTTTCAAA TAACATCTTT TGATGGTAAA AAGTCTTCAA TCTTACAGGC AGAGAGTAAA 
AAAGATTATG AAGAGTGGAT ATGCACAATA AACAACATAT CTAAACAGAT ATATCTAAGT 
GAAAACCCTG AGGAAATTGC TGCACGTGTA AACCAGTCAG CTCTCGAGGC CGTTACTCCA 
TCACCTTCAT TTCAGCAGAG GCATGAGAGC ATGCGTCCGA CTGTACAATC TCGGCCTCCC 
ACAGCCCGCA CGAGCAGCAC GGGGTCACTG GGATCCGAAT CCGCCGCTCT GTCTGCACTG 
TCCTTGGATT CACTCGTTGC CCCTGACACT CCTATTCAAT TTGACATAAT ATCTCCAGTT 
AGTGAAGATC TGCCTGGTCA AGCAAAGTCT TCAGGGCAGT CAGGCAGACG CACAAATCCT 
TTTGGTGAAA CGGGAGGCTC AAAATCTGAA ACTGAAGATT CAATTCTTCA TCAGTTGTTC 
ATTGTAAGGT TTCTTGGCTC GATGGAGGTG AAGTCTGATG AAAGTCCAGA TGTTGTTTAT 
GAAACAATGC GTCAAATCCT AGCAGCTCGG GCCATCCATA ACATTTTCAG GATGACAGAA 
TCACATTTAT TAGTCACTTG TGACTGTTTA AAGTTAATTG ATCCACAGAC ACAAGTTACA 
AGACTACGAT TTCCTTTGCC CAATGTAGTC TTGTATGCTA CACATCAGGA GAATAAGCGC 
CTCTTTGGAT TTGTGCTCAG AACTTCAGGA GGGAGAGCTG ACAGTCGACA AACTTCCATC 
TGCTATATCT TTGAATCAAA CAATGAAGGG GAAAAGATTT GTGACTCCGT TGGACTGGCA 
AAACAGATTG CTTTCCATGC AGAACTGGAT CGAAAAGCAT CAGAAAAGCA AAAAGAAATG 
GAGAGAGTCA AAGAGAAACA ACAAAAAGAA CTGACTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAGGAGC AAAGCCGACT GATAGCTGCT TCCAACAGAT CAAACCCTAG CAGTGGAGAA 
GGACAGTTTG TCGTCCTTAG CAGCAGCCAG TCAGAAGACA GCGATTTAGG AGAGGATGGA 
AAGAAGAAAA GGGAATCTGA AACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004944641.2
CDS	126..2252
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDAAAISNYVNQLFQAMHRVYDAQNELSAATHLTSKLLKEYEKQHFPLGGDDEIMSSTLQQFAKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAITRYSRLSKRRENEKIKAEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIAVLEPLLGYMQAQISFFKMGSENLTEQLEEFLTNIGTSVQNVRREMECEIENMQQTIEDLEVASDPLYLPDPDPTKFPVHRNLTRKAGYLNARNKTGLVSSSWERQFYFTQGGNLMSQARGDVAGGLVMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDYEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESMRPTVQSRPPTARTSSTGSLGSESAALSALSLDSLVAPDTPIQFDIISPVSEDLPGQAKSSGQSGRRTNPFGETGGSKSETEDSILHQLFIVRFLGSMEVKSDESPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLRFPLPNVVLYATHQENKRLFGFVLRTSGGRADSRQTSICYIFESNNEGEKICDSVGLAKQIAFHAELDRKASEKQKEMERVKEKQQKELTKQKQIEKDLEEQSRLIAASNRSNPSSGEGQFVVLSSSQSEDSDLGEDGKKKRESET

Target ORF information:

RefSeq Version	XM_004944584.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004944584.2

ORF Insert Sequence:

ATGCCCGGCA TCGACAAGCT GCCCATCGAG GAGACGCTGG AAGACAGCCC CCAGACCCGT 
TCTTTGCTGG GAGTATTTGA AGAAGATGCT GCTGCAATTT CCAACTACGT CAATCAGTTA 
TTTCAAGCAA TGCATAGAGT ATATGATGCA CAGAATGAAT TAAGTGCAGC GACTCATCTG 
ACTTCAAAGC TTCTGAAGGA ATATGAGAAA CAGCACTTTC CACTAGGGGG AGATGATGAA 
ATTATGAGTT CTACTTTACA GCAATTTGCA AAAGTGATCG ACGAGCTCAG TTCTTGCCAT 
GCAGTACTTT CAACTCAACT TGCAGATGCA ATGATGTTTC CTATTACTCA GTTTAAAGAA 
AGAGATCTAA AAGAGATATT AACTCTAAAA GAAGTTTTCC AAATTGCAAG CAATGACCAC 
GACGCTGCCA TTACCCGATA CAGTCGGTTG TCAAAAAGAA GGGAAAATGA GAAGATCAAG 
GCTGAAGTTA CAGAAGATGT GTATACTTCC AGGAAAAAAC AGCATCAGAC TATGATGCAT 
TATTTCTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TTGCTGTGTT AGAACCCTTG 
CTAGGATACA TGCAAGCTCA GATAAGCTTT TTTAAAATGG GTTCAGAAAA TCTCACTGAG 
CAACTGGAGG AATTTCTCAC CAACATTGGC ACAAGTGTAC AAAATGTTCG CAGGGAAATG 
GAGTGCGAAA TAGAGAACAT GCAGCAAACT ATAGAAGACT TGGAAGTAGC CAGTGATCCA 
CTTTATTTGC CTGATCCGGA TCCTACGAAA TTTCCTGTCC ATAGAAATCT TACACGGAAA 
GCTGGCTATC TCAATGCAAG AAATAAAACA GGGCTGGTAT CTTCCAGTTG GGAGAGACAG 
TTCTACTTCA CTCAAGGTGG AAATCTGATG AGCCAGGCAA GAGGTGATGT GGCTGGGGGA 
CTTGTCATGG ACATAGACAA TTGTTCAGTA ATGGCTGTGG ACTGCGAAGA CAGACGGTAC 
TGTTTTCAAA TAACATCTTT TGATGGTAAA AAGTCTTCAA TCTTACAGGC AGAGAGTAAA 
AAAGATTATG AAGAGTGGAT ATGCACAATA AACAACATAT CTAAACAGAT ATATCTAAGT 
GAAAACCCTG AGGAAATTGC TGCACGTGTA AACCAGTCAG CTCTCGAGGC CGTTACTCCA 
TCACCTTCAT TTCAGCAGAG GCATGAGAGC ATGCGTCCGA CTGTACAATC TCGGCCTCCC 
ACAGCCCGCA CGAGCAGCAC GGGGTCACTG GGATCCGAAT CCGCCGCTCT GTCTGCACTG 
TCCTTGGATT CACTCGTTGC CCCTGACACT CCTATTCAAT TTGACATAAT ATCTCCAGTT 
AGTGAAGATC TGCCTGGTCA AGCAAAGTCT TCAGGGCAGT CAGGCAGACG CACAAATCCT 
TTTGGTGAAA CGGGAGGCTC AAAATCTGAA ACTGAAGATT CAATTCTTCA TCAGTTGTTC 
ATTGTAAGGT TTCTTGGCTC GATGGAGGTG AAGTCTGATG AAAGTCCAGA TGTTGTTTAT 
GAAACAATGC GTCAAATCCT AGCAGCTCGG GCCATCCATA ACATTTTCAG GATGACAGAA 
TCACATTTAT TAGTCACTTG TGACTGTTTA AAGTTAATTG ATCCACAGAC ACAAGTTACA 
AGACTACGAT TTCCTTTGCC CAATGTAGTC TTGTATGCTA CACATCAGGA GAATAAGCGC 
CTCTTTGGAT TTGTGCTCAG AACTTCAGGA GGGAGAGCTG ACAGTCGACA AACTTCCATC 
TGCTATATCT TTGAATCAAA CAATGAAGGG GAAAAGATTT GTGACTCCGT TGGACTGGCA 
AAACAGATTG CTTTCCATGC AGAACTGGAT CGAAAAGCAT CAGAAAAGCA AAAAGAAATG 
GAGAGAGTCA AAGAGAAACA ACAAAAAGAA CTGACTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAGGAGC AAAGCCGACT GATAGCTGCT TCCAACAGAT CAAACCCTAG CAGTGGAGAA 
GGACAGTTTG TCGTCCTTAG CAGCAGCCAG TCAGAAGACA GCGATTTAGG AGAGGATGGA 
AAGAAGAAAA GGGAATCTGA AACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APPL1 ( XM_414344.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_414344.5 Gallus gallus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X2, mRNA.

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CloneID	OGa41640
Clone ID Related Accession (Same CDS sequence)	XM_004944584.3 , XM_414344.5 , XM_004944584.2 , XM_414344.6
Accession Version	XM_414344.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	DCC-interacting protein 13-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_414344.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGCA TCGACAAGCT GCCCATCGAG GAGACGCTGG AAGACAGCCC CCAGACCCGT 
TCTTTGCTGG GAGTATTTGA AGAAGATGCT GCTGCAATTT CCAACTACGT CAATCAGTTA 
TTTCAAGCAA TGCATAGAGT ATATGATGCA CAGAATGAAT TAAGTGCAGC GACTCATCTG 
ACTTCAAAGC TTCTGAAGGA ATATGAGAAA CAGCACTTTC CACTAGGGGG AGATGATGAA 
ATTATGAGTT CTACTTTACA GCAATTTGCA AAAGTGATCG ACGAGCTCAG TTCTTGCCAT 
GCAGTACTTT CAACTCAACT TGCAGATGCA ATGATGTTTC CTATTACTCA GTTTAAAGAA 
AGAGATCTAA AAGAGATATT AACTCTAAAA GAAGTTTTCC AAATTGCAAG CAATGACCAC 
GACGCTGCCA TTACCCGATA CAGTCGGTTG TCAAAAAGAA GGGAAAATGA GAAGATCAAG 
GCTGAAGTTA CAGAAGATGT GTATACTTCC AGGAAAAAAC AGCATCAGAC TATGATGCAT 
TATTTCTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TTGCTGTGTT AGAACCCTTG 
CTAGGATACA TGCAAGCTCA GATAAGCTTT TTTAAAATGG GTTCAGAAAA TCTCACTGAG 
CAACTGGAGG AATTTCTCAC CAACATTGGC ACAAGTGTAC AAAATGTTCG CAGGGAAATG 
GAGTGCGAAA TAGAGAACAT GCAGCAAACT ATAGAAGACT TGGAAGTAGC CAGTGATCCA 
CTTTATTTGC CTGATCCGGA TCCTACGAAA TTTCCTGTCC ATAGAAATCT TACACGGAAA 
GCTGGCTATC TCAATGCAAG AAATAAAACA GGGCTGGTAT CTTCCAGTTG GGAGAGACAG 
TTCTACTTCA CTCAAGGTGG AAATCTGATG AGCCAGGCAA GAGGTGATGT GGCTGGGGGA 
CTTGTCATGG ACATAGACAA TTGTTCAGTA ATGGCTGTGG ACTGCGAAGA CAGACGGTAC 
TGTTTTCAAA TAACATCTTT TGATGGTAAA AAGTCTTCAA TCTTACAGGC AGAGAGTAAA 
AAAGATTATG AAGAGTGGAT ATGCACAATA AACAACATAT CTAAACAGAT ATATCTAAGT 
GAAAACCCTG AGGAAATTGC TGCACGTGTA AACCAGTCAG CTCTCGAGGC CGTTACTCCA 
TCACCTTCAT TTCAGCAGAG GCATGAGAGC ATGCGTCCGA CTGTACAATC TCGGCCTCCC 
ACAGCCCGCA CGAGCAGCAC GGGGTCACTG GGATCCGAAT CCGCCGCTCT GTCTGCACTG 
TCCTTGGATT CACTCGTTGC CCCTGACACT CCTATTCAAT TTGACATAAT ATCTCCAGTT 
AGTGAAGATC TGCCTGGTCA AGCAAAGTCT TCAGGGCAGT CAGGCAGACG CACAAATCCT 
TTTGGTGAAA CGGGAGGCTC AAAATCTGAA ACTGAAGATT CAATTCTTCA TCAGTTGTTC 
ATTGTAAGGT TTCTTGGCTC GATGGAGGTG AAGTCTGATG AAAGTCCAGA TGTTGTTTAT 
GAAACAATGC GTCAAATCCT AGCAGCTCGG GCCATCCATA ACATTTTCAG GATGACAGAA 
TCACATTTAT TAGTCACTTG TGACTGTTTA AAGTTAATTG ATCCACAGAC ACAAGTTACA 
AGACTACGAT TTCCTTTGCC CAATGTAGTC TTGTATGCTA CACATCAGGA GAATAAGCGC 
CTCTTTGGAT TTGTGCTCAG AACTTCAGGA GGGAGAGCTG ACAGTCGACA AACTTCCATC 
TGCTATATCT TTGAATCAAA CAATGAAGGG GAAAAGATTT GTGACTCCGT TGGACTGGCA 
AAACAGATTG CTTTCCATGC AGAACTGGAT CGAAAAGCAT CAGAAAAGCA AAAAGAAATG 
GAGAGAGTCA AAGAGAAACA ACAAAAAGAA CTGACTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAGGAGC AAAGCCGACT GATAGCTGCT TCCAACAGAT CAAACCCTAG CAGTGGAGAA 
GGACAGTTTG TCGTCCTTAG CAGCAGCCAG TCAGAAGACA GCGATTTAGG AGAGGATGGA 
AAGAAGAAAA GGGAATCTGA AACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_414344.5
CDS	88..2214
Translation	MPGIDKLPIEETLEDSPQTRSLLGVFEEDAAAISNYVNQLFQAMHRVYDAQNELSAATHLTSKLLKEYEKQHFPLGGDDEIMSSTLQQFAKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAITRYSRLSKRRENEKIKAEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIAVLEPLLGYMQAQISFFKMGSENLTEQLEEFLTNIGTSVQNVRREMECEIENMQQTIEDLEVASDPLYLPDPDPTKFPVHRNLTRKAGYLNARNKTGLVSSSWERQFYFTQGGNLMSQARGDVAGGLVMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDYEEWICTINNISKQIYLSENPEEIAARVNQSALEAVTPSPSFQQRHESMRPTVQSRPPTARTSSTGSLGSESAALSALSLDSLVAPDTPIQFDIISPVSEDLPGQAKSSGQSGRRTNPFGETGGSKSETEDSILHQLFIVRFLGSMEVKSDESPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLRFPLPNVVLYATHQENKRLFGFVLRTSGGRADSRQTSICYIFESNNEGEKICDSVGLAKQIAFHAELDRKASEKQKEMERVKEKQQKELTKQKQIEKDLEEQSRLIAASNRSNPSSGEGQFVVLSSSQSEDSDLGEDGKKKRESET

Target ORF information:

RefSeq Version	XM_414344.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_414344.6

ORF Insert Sequence:

ATGCCCGGCA TCGACAAGCT GCCCATCGAG GAGACGCTGG AAGACAGCCC CCAGACCCGT 
TCTTTGCTGG GAGTATTTGA AGAAGATGCT GCTGCAATTT CCAACTACGT CAATCAGTTA 
TTTCAAGCAA TGCATAGAGT ATATGATGCA CAGAATGAAT TAAGTGCAGC GACTCATCTG 
ACTTCAAAGC TTCTGAAGGA ATATGAGAAA CAGCACTTTC CACTAGGGGG AGATGATGAA 
ATTATGAGTT CTACTTTACA GCAATTTGCA AAAGTGATCG ACGAGCTCAG TTCTTGCCAT 
GCAGTACTTT CAACTCAACT TGCAGATGCA ATGATGTTTC CTATTACTCA GTTTAAAGAA 
AGAGATCTAA AAGAGATATT AACTCTAAAA GAAGTTTTCC AAATTGCAAG CAATGACCAC 
GACGCTGCCA TTACCCGATA CAGTCGGTTG TCAAAAAGAA GGGAAAATGA GAAGATCAAG 
GCTGAAGTTA CAGAAGATGT GTATACTTCC AGGAAAAAAC AGCATCAGAC TATGATGCAT 
TATTTCTGTG CACTAAATAC TCTTCAGTAC AAGAAGAAAA TTGCTGTGTT AGAACCCTTG 
CTAGGATACA TGCAAGCTCA GATAAGCTTT TTTAAAATGG GTTCAGAAAA TCTCACTGAG 
CAACTGGAGG AATTTCTCAC CAACATTGGC ACAAGTGTAC AAAATGTTCG CAGGGAAATG 
GAGTGCGAAA TAGAGAACAT GCAGCAAACT ATAGAAGACT TGGAAGTAGC CAGTGATCCA 
CTTTATTTGC CTGATCCGGA TCCTACGAAA TTTCCTGTCC ATAGAAATCT TACACGGAAA 
GCTGGCTATC TCAATGCAAG AAATAAAACA GGGCTGGTAT CTTCCAGTTG GGAGAGACAG 
TTCTACTTCA CTCAAGGTGG AAATCTGATG AGCCAGGCAA GAGGTGATGT GGCTGGGGGA 
CTTGTCATGG ACATAGACAA TTGTTCAGTA ATGGCTGTGG ACTGCGAAGA CAGACGGTAC 
TGTTTTCAAA TAACATCTTT TGATGGTAAA AAGTCTTCAA TCTTACAGGC AGAGAGTAAA 
AAAGATTATG AAGAGTGGAT ATGCACAATA AACAACATAT CTAAACAGAT ATATCTAAGT 
GAAAACCCTG AGGAAATTGC TGCACGTGTA AACCAGTCAG CTCTCGAGGC CGTTACTCCA 
TCACCTTCAT TTCAGCAGAG GCATGAGAGC ATGCGTCCGA CTGTACAATC TCGGCCTCCC 
ACAGCCCGCA CGAGCAGCAC GGGGTCACTG GGATCCGAAT CCGCCGCTCT GTCTGCACTG 
TCCTTGGATT CACTCGTTGC CCCTGACACT CCTATTCAAT TTGACATAAT ATCTCCAGTT 
AGTGAAGATC TGCCTGGTCA AGCAAAGTCT TCAGGGCAGT CAGGCAGACG CACAAATCCT 
TTTGGTGAAA CGGGAGGCTC AAAATCTGAA ACTGAAGATT CAATTCTTCA TCAGTTGTTC 
ATTGTAAGGT TTCTTGGCTC GATGGAGGTG AAGTCTGATG AAAGTCCAGA TGTTGTTTAT 
GAAACAATGC GTCAAATCCT AGCAGCTCGG GCCATCCATA ACATTTTCAG GATGACAGAA 
TCACATTTAT TAGTCACTTG TGACTGTTTA AAGTTAATTG ATCCACAGAC ACAAGTTACA 
AGACTACGAT TTCCTTTGCC CAATGTAGTC TTGTATGCTA CACATCAGGA GAATAAGCGC 
CTCTTTGGAT TTGTGCTCAG AACTTCAGGA GGGAGAGCTG ACAGTCGACA AACTTCCATC 
TGCTATATCT TTGAATCAAA CAATGAAGGG GAAAAGATTT GTGACTCCGT TGGACTGGCA 
AAACAGATTG CTTTCCATGC AGAACTGGAT CGAAAAGCAT CAGAAAAGCA AAAAGAAATG 
GAGAGAGTCA AAGAGAAACA ACAAAAAGAA CTGACTAAAC AAAAACAAAT TGAAAAGGAC 
TTAGAGGAGC AAAGCCGACT GATAGCTGCT TCCAACAGAT CAAACCCTAG CAGTGGAGAA 
GGACAGTTTG TCGTCCTTAG CAGCAGCCAG TCAGAAGACA GCGATTTAGG AGAGGATGGA 
AAGAAGAAAA GGGAATCTGA AACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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APPL1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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