CHRNB4 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following CHRNB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu02609 NM_000750.5
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu56898 XM_017021886.1
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu56898 XM_017021885.1
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu56900 XM_011521191.2
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu56900 XM_011521190.2
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu56901 XM_011521192.2
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu10996 NM_001256567.3
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu56897 XM_011521186.2
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu56897 XM_011521187.2
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu85239 XM_017021887.1
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu85240 XM_017021888.1
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu85241 XM_017021889.2
Latest version!
Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OHu02609 NM_000750.3 Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu85241 XM_017021889.1 Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu10996 NM_001256567.1 Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu10996 NM_001256567.2 Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu02609 NM_000750.4 Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu02609
    Clone ID Related Accession (Same CDS sequence) NM_000750.3 , NM_000750.4 , NM_000750.5
    Accession Version NM_000750.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-05-03
    Organism Homo sapiens(Human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform 1 precursor
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC067863.19. On Jul 10, 2008 this sequence version replaced NM_000750.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U48861.1, SRR1803614.91133.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT CGTTGAGGAC TGGAAGTACG TGGCTATGGT GGTGGACCGG
    CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC GTCCTGGGCA CTGTGGGGCT CTTCCTACCG
    CCCCTCTTCC AGACCCATGC AGCTTCTGAG GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000741.1
    CDS113..1609
    Translation

    Target ORF information:

    RefSeq Version NM_000750.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000750.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT CGTTGAGGAC TGGAAGTACG TGGCTATGGT GGTGGACCGG
    CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC GTCCTGGGCA CTGTGGGGCT CTTCCTACCG
    CCCCTCTTCC AGACCCATGC AGCTTCTGAG GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02609
    Clone ID Related Accession (Same CDS sequence) NM_000750.3 , NM_000750.4 , NM_000750.5
    Accession Version NM_000750.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-10
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC067863.19, CN366252.1, BX348201.1, BF311784.1, BC096081.1, HY213565.1, U62439.1, BY795907.2, U48861.1 and AI625439.1. On Sep 2, 2017 this sequence version replaced NM_000750.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U48861.1, U62439.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT CGTTGAGGAC TGGAAGTACG TGGCTATGGT GGTGGACCGG
    CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC GTCCTGGGCA CTGTGGGGCT CTTCCTACCG
    CCCCTCTTCC AGACCCATGC AGCTTCTGAG GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000741.1
    CDS113..1609
    Translation

    Target ORF information:

    RefSeq Version NM_000750.4
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000750.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT CGTTGAGGAC TGGAAGTACG TGGCTATGGT GGTGGACCGG
    CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC GTCCTGGGCA CTGTGGGGCT CTTCCTACCG
    CCCCTCTTCC AGACCCATGC AGCTTCTGAG GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02609
    Clone ID Related Accession (Same CDS sequence) NM_000750.3 , NM_000750.4 , NM_000750.5
    Accession Version NM_000750.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC067863.19, CN366252.1, BX348201.1, BF311784.1, BC096081.1, HY213565.1, U62439.1, BY795907.2, U48861.1 and AI625439.1. On Nov 22, 2018 this sequence version replaced NM_000750.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.91133.1, U48861.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261751.8/ ENSP00000261751.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT CGTTGAGGAC TGGAAGTACG TGGCTATGGT GGTGGACCGG
    CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC GTCCTGGGCA CTGTGGGGCT CTTCCTACCG
    CCCCTCTTCC AGACCCATGC AGCTTCTGAG GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000741.1
    CDS78..1574
    Translation

    Target ORF information:

    RefSeq Version NM_000750.5
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000750.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT CGTTGAGGAC TGGAAGTACG TGGCTATGGT GGTGGACCGG
    CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC GTCCTGGGCA CTGTGGGGCT CTTCCTACCG
    CCCCTCTTCC AGACCCATGC AGCTTCTGAG GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56898
    Clone ID Related Accession (Same CDS sequence) XM_017021885.1 , XM_017021886.1
    Accession Version XM_017021885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGACGACC TTCTGAACAA AACCCGTTAC AATAACCTGA TCCGCCCAGC CACCAGCTCC 
    TCACAGCTCA TCTCCATCAA GCTGCAGCTC TCCCTGGCCC AGCTTATCAG CGTGAATGAG
    CGAGAGCAGA TCATGACCAC CAATGTCTGG CTGAAACAGG AATGGACTGA TTACCGCCTG
    ACCTGGAACA GCTCCCGCTA CGAGGGTGTG AACATCCTGA GGATCCCTGC AAAGCGCATC
    TGGTTGCCTG ACATCGTGCT TTACAACAAC GCCGACGGGA CCTATGAGGT GTCTGTCTAC
    ACCAACTTGA TAGTCCGGTC CAACGGCAGC GTCCTGTGGC TGCCCCCTGC CATCTACAAG
    AGCGCCTGCA AGATTGAGGT GAAGTACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAG
    TTCCGCTCCT GGACCTATGA CCACACGGAG ATAGACATGG TCCTCATGAC GCCCACAGCC
    AGCATGGATG ACTTTACTCC CAGTGGTGAG TGGGACATAG TGGCCCTCCC AGGGAGAAGG
    ACAGTGAACC CACAAGACCC CAGCTACGTG GACGTGACTT ACGACTTCAT CATCAAGCGC
    AAGCCTCTGT TCTACACCAT CAACCTCATC ATCCCCTGCG TGCTCACCAC CTTGCTGGCC
    ATCCTCGTCT TCTACCTGCC ATCCGACTGC GGCGAGAAGA TGACACTGTG CATCTCAGTG
    CTGCTGGCAC TGACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCACC CACCTCCCTC
    GATGTGCCTC TCATCGGCAA GTACCTCATG TTCACCATGG TGCTGGTCAC CTTCTCCATC
    GTCACCAGCG TCTGTGTGCT CAATGTGCAC CACCGCTCGC CCAGCACCCA CACCATGGCA
    CCCTGGGTCA AGCGCTGCTT CCTGCACAAG CTGCCTACCT TCCTCTTCAT GAAGCGCCCT
    GGCCCCGACA GCAGCCCGGC CAGAGCCTTC CCGCCCAGCA AGTCATGCGT GACCAAGCCC
    GAGGCCACCG CCACCTCCAC CAGCCCCTCC AACTTCTATG GGAACTCCAT GTACTTTGTG
    AACCCCGCCT CTGCAGCTTC CAAGTCTCCA GCCGGCTCTA CCCCGGTGGC TATCCCCAGG
    GATTTCTGGC TGCGGTCCTC TGGGAGGTTC CGACAGGATG TGCAGGAGGC ATTAGAAGGT
    GTCAGCTTCA TCGCCCAGCA CATGAAGAAT GACGATGAAG ACCAGAGTGT CGTTGAGGAC
    TGGAAGTACG TGGCTATGGT GGTGGACCGG CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC
    GTCCTGGGCA CTGTGGGGCT CTTCCTACCG CCCCTCTTCC AGACCCATGC AGCTTCTGAG
    GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877374.1
    CDS298..1704
    Translation

    Target ORF information:

    RefSeq Version XM_017021885.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGACGACC TTCTGAACAA AACCCGTTAC AATAACCTGA TCCGCCCAGC CACCAGCTCC 
    TCACAGCTCA TCTCCATCAA GCTGCAGCTC TCCCTGGCCC AGCTTATCAG CGTGAATGAG
    CGAGAGCAGA TCATGACCAC CAATGTCTGG CTGAAACAGG AATGGACTGA TTACCGCCTG
    ACCTGGAACA GCTCCCGCTA CGAGGGTGTG AACATCCTGA GGATCCCTGC AAAGCGCATC
    TGGTTGCCTG ACATCGTGCT TTACAACAAC GCCGACGGGA CCTATGAGGT GTCTGTCTAC
    ACCAACTTGA TAGTCCGGTC CAACGGCAGC GTCCTGTGGC TGCCCCCTGC CATCTACAAG
    AGCGCCTGCA AGATTGAGGT GAAGTACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAG
    TTCCGCTCCT GGACCTATGA CCACACGGAG ATAGACATGG TCCTCATGAC GCCCACAGCC
    AGCATGGATG ACTTTACTCC CAGTGGTGAG TGGGACATAG TGGCCCTCCC AGGGAGAAGG
    ACAGTGAACC CACAAGACCC CAGCTACGTG GACGTGACTT ACGACTTCAT CATCAAGCGC
    AAGCCTCTGT TCTACACCAT CAACCTCATC ATCCCCTGCG TGCTCACCAC CTTGCTGGCC
    ATCCTCGTCT TCTACCTGCC ATCCGACTGC GGCGAGAAGA TGACACTGTG CATCTCAGTG
    CTGCTGGCAC TGACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCACC CACCTCCCTC
    GATGTGCCTC TCATCGGCAA GTACCTCATG TTCACCATGG TGCTGGTCAC CTTCTCCATC
    GTCACCAGCG TCTGTGTGCT CAATGTGCAC CACCGCTCGC CCAGCACCCA CACCATGGCA
    CCCTGGGTCA AGCGCTGCTT CCTGCACAAG CTGCCTACCT TCCTCTTCAT GAAGCGCCCT
    GGCCCCGACA GCAGCCCGGC CAGAGCCTTC CCGCCCAGCA AGTCATGCGT GACCAAGCCC
    GAGGCCACCG CCACCTCCAC CAGCCCCTCC AACTTCTATG GGAACTCCAT GTACTTTGTG
    AACCCCGCCT CTGCAGCTTC CAAGTCTCCA GCCGGCTCTA CCCCGGTGGC TATCCCCAGG
    GATTTCTGGC TGCGGTCCTC TGGGAGGTTC CGACAGGATG TGCAGGAGGC ATTAGAAGGT
    GTCAGCTTCA TCGCCCAGCA CATGAAGAAT GACGATGAAG ACCAGAGTGT CGTTGAGGAC
    TGGAAGTACG TGGCTATGGT GGTGGACCGG CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC
    GTCCTGGGCA CTGTGGGGCT CTTCCTACCG CCCCTCTTCC AGACCCATGC AGCTTCTGAG
    GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56898
    Clone ID Related Accession (Same CDS sequence) XM_017021885.1 , XM_017021886.1
    Accession Version XM_017021886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGACGACC TTCTGAACAA AACCCGTTAC AATAACCTGA TCCGCCCAGC CACCAGCTCC 
    TCACAGCTCA TCTCCATCAA GCTGCAGCTC TCCCTGGCCC AGCTTATCAG CGTGAATGAG
    CGAGAGCAGA TCATGACCAC CAATGTCTGG CTGAAACAGG AATGGACTGA TTACCGCCTG
    ACCTGGAACA GCTCCCGCTA CGAGGGTGTG AACATCCTGA GGATCCCTGC AAAGCGCATC
    TGGTTGCCTG ACATCGTGCT TTACAACAAC GCCGACGGGA CCTATGAGGT GTCTGTCTAC
    ACCAACTTGA TAGTCCGGTC CAACGGCAGC GTCCTGTGGC TGCCCCCTGC CATCTACAAG
    AGCGCCTGCA AGATTGAGGT GAAGTACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAG
    TTCCGCTCCT GGACCTATGA CCACACGGAG ATAGACATGG TCCTCATGAC GCCCACAGCC
    AGCATGGATG ACTTTACTCC CAGTGGTGAG TGGGACATAG TGGCCCTCCC AGGGAGAAGG
    ACAGTGAACC CACAAGACCC CAGCTACGTG GACGTGACTT ACGACTTCAT CATCAAGCGC
    AAGCCTCTGT TCTACACCAT CAACCTCATC ATCCCCTGCG TGCTCACCAC CTTGCTGGCC
    ATCCTCGTCT TCTACCTGCC ATCCGACTGC GGCGAGAAGA TGACACTGTG CATCTCAGTG
    CTGCTGGCAC TGACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCACC CACCTCCCTC
    GATGTGCCTC TCATCGGCAA GTACCTCATG TTCACCATGG TGCTGGTCAC CTTCTCCATC
    GTCACCAGCG TCTGTGTGCT CAATGTGCAC CACCGCTCGC CCAGCACCCA CACCATGGCA
    CCCTGGGTCA AGCGCTGCTT CCTGCACAAG CTGCCTACCT TCCTCTTCAT GAAGCGCCCT
    GGCCCCGACA GCAGCCCGGC CAGAGCCTTC CCGCCCAGCA AGTCATGCGT GACCAAGCCC
    GAGGCCACCG CCACCTCCAC CAGCCCCTCC AACTTCTATG GGAACTCCAT GTACTTTGTG
    AACCCCGCCT CTGCAGCTTC CAAGTCTCCA GCCGGCTCTA CCCCGGTGGC TATCCCCAGG
    GATTTCTGGC TGCGGTCCTC TGGGAGGTTC CGACAGGATG TGCAGGAGGC ATTAGAAGGT
    GTCAGCTTCA TCGCCCAGCA CATGAAGAAT GACGATGAAG ACCAGAGTGT CGTTGAGGAC
    TGGAAGTACG TGGCTATGGT GGTGGACCGG CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC
    GTCCTGGGCA CTGTGGGGCT CTTCCTACCG CCCCTCTTCC AGACCCATGC AGCTTCTGAG
    GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877375.1
    CDS2105..3511
    Translation

    Target ORF information:

    RefSeq Version XM_017021886.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGACGACC TTCTGAACAA AACCCGTTAC AATAACCTGA TCCGCCCAGC CACCAGCTCC 
    TCACAGCTCA TCTCCATCAA GCTGCAGCTC TCCCTGGCCC AGCTTATCAG CGTGAATGAG
    CGAGAGCAGA TCATGACCAC CAATGTCTGG CTGAAACAGG AATGGACTGA TTACCGCCTG
    ACCTGGAACA GCTCCCGCTA CGAGGGTGTG AACATCCTGA GGATCCCTGC AAAGCGCATC
    TGGTTGCCTG ACATCGTGCT TTACAACAAC GCCGACGGGA CCTATGAGGT GTCTGTCTAC
    ACCAACTTGA TAGTCCGGTC CAACGGCAGC GTCCTGTGGC TGCCCCCTGC CATCTACAAG
    AGCGCCTGCA AGATTGAGGT GAAGTACTTT CCCTTCGACC AGCAGAACTG CACCCTCAAG
    TTCCGCTCCT GGACCTATGA CCACACGGAG ATAGACATGG TCCTCATGAC GCCCACAGCC
    AGCATGGATG ACTTTACTCC CAGTGGTGAG TGGGACATAG TGGCCCTCCC AGGGAGAAGG
    ACAGTGAACC CACAAGACCC CAGCTACGTG GACGTGACTT ACGACTTCAT CATCAAGCGC
    AAGCCTCTGT TCTACACCAT CAACCTCATC ATCCCCTGCG TGCTCACCAC CTTGCTGGCC
    ATCCTCGTCT TCTACCTGCC ATCCGACTGC GGCGAGAAGA TGACACTGTG CATCTCAGTG
    CTGCTGGCAC TGACATTCTT CCTGCTGCTC ATCTCCAAGA TCGTGCCACC CACCTCCCTC
    GATGTGCCTC TCATCGGCAA GTACCTCATG TTCACCATGG TGCTGGTCAC CTTCTCCATC
    GTCACCAGCG TCTGTGTGCT CAATGTGCAC CACCGCTCGC CCAGCACCCA CACCATGGCA
    CCCTGGGTCA AGCGCTGCTT CCTGCACAAG CTGCCTACCT TCCTCTTCAT GAAGCGCCCT
    GGCCCCGACA GCAGCCCGGC CAGAGCCTTC CCGCCCAGCA AGTCATGCGT GACCAAGCCC
    GAGGCCACCG CCACCTCCAC CAGCCCCTCC AACTTCTATG GGAACTCCAT GTACTTTGTG
    AACCCCGCCT CTGCAGCTTC CAAGTCTCCA GCCGGCTCTA CCCCGGTGGC TATCCCCAGG
    GATTTCTGGC TGCGGTCCTC TGGGAGGTTC CGACAGGATG TGCAGGAGGC ATTAGAAGGT
    GTCAGCTTCA TCGCCCAGCA CATGAAGAAT GACGATGAAG ACCAGAGTGT CGTTGAGGAC
    TGGAAGTACG TGGCTATGGT GGTGGACCGG CTGTTCCTGT GGGTGTTCAT GTTTGTGTGC
    GTCCTGGGCA CTGTGGGGCT CTTCCTACCG CCCCTCTTCC AGACCCATGC AGCTTCTGAG
    GGGCCCTACG CTGCCCAGCG TGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56900
    Clone ID Related Accession (Same CDS sequence) XM_011521190.2 , XM_011521191.2
    Accession Version XM_011521190.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACCACCA ATGTCTGGCT GAAACAGGAA TGGACTGATT ACCGCCTGAC CTGGAACAGC 
    TCCCGCTACG AGGGTGTGAA CATCCTGAGG ATCCCTGCAA AGCGCATCTG GTTGCCTGAC
    ATCGTGCTTT ACAACAACGC CGACGGGACC TATGAGGTGT CTGTCTACAC CAACTTGATA
    GTCCGGTCCA ACGGCAGCGT CCTGTGGCTG CCCCCTGCCA TCTACAAGAG CGCCTGCAAG
    ATTGAGGTGA AGTACTTTCC CTTCGACCAG CAGAACTGCA CCCTCAAGTT CCGCTCCTGG
    ACCTATGACC ACACGGAGAT AGACATGGTC CTCATGACGC CCACAGCCAG CATGGATGAC
    TTTACTCCCA GTGGTGAGTG GGACATAGTG GCCCTCCCAG GGAGAAGGAC AGTGAACCCA
    CAAGACCCCA GCTACGTGGA CGTGACTTAC GACTTCATCA TCAAGCGCAA GCCTCTGTTC
    TACACCATCA ACCTCATCAT CCCCTGCGTG CTCACCACCT TGCTGGCCAT CCTCGTCTTC
    TACCTGCCAT CCGACTGCGG CGAGAAGATG ACACTGTGCA TCTCAGTGCT GCTGGCACTG
    ACATTCTTCC TGCTGCTCAT CTCCAAGATC GTGCCACCCA CCTCCCTCGA TGTGCCTCTC
    ATCGGCAAGT ACCTCATGTT CACCATGGTG CTGGTCACCT TCTCCATCGT CACCAGCGTC
    TGTGTGCTCA ATGTGCACCA CCGCTCGCCC AGCACCCACA CCATGGCACC CTGGGTCAAG
    CGCTGCTTCC TGCACAAGCT GCCTACCTTC CTCTTCATGA AGCGCCCTGG CCCCGACAGC
    AGCCCGGCCA GAGCCTTCCC GCCCAGCAAG TCATGCGTGA CCAAGCCCGA GGCCACCGCC
    ACCTCCACCA GCCCCTCCAA CTTCTATGGG AACTCCATGT ACTTTGTGAA CCCCGCCTCT
    GCAGCTTCCA AGTCTCCAGC CGGCTCTACC CCGGTGGCTA TCCCCAGGGA TTTCTGGCTG
    CGGTCCTCTG GGAGGTTCCG ACAGGATGTG CAGGAGGCAT TAGAAGGTGT CAGCTTCATC
    GCCCAGCACA TGAAGAATGA CGATGAAGAC CAGAGTGTCG TTGAGGACTG GAAGTACGTG
    GCTATGGTGG TGGACCGGCT GTTCCTGTGG GTGTTCATGT TTGTGTGCGT CCTGGGCACT
    GTGGGGCTCT TCCTACCGCC CCTCTTCCAG ACCCATGCAG CTTCTGAGGG GCCCTACGCT
    GCCCAGCGTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519492.1
    CDS503..1777
    Translation

    Target ORF information:

    RefSeq Version XM_011521190.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521190.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACCACCA ATGTCTGGCT GAAACAGGAA TGGACTGATT ACCGCCTGAC CTGGAACAGC 
    TCCCGCTACG AGGGTGTGAA CATCCTGAGG ATCCCTGCAA AGCGCATCTG GTTGCCTGAC
    ATCGTGCTTT ACAACAACGC CGACGGGACC TATGAGGTGT CTGTCTACAC CAACTTGATA
    GTCCGGTCCA ACGGCAGCGT CCTGTGGCTG CCCCCTGCCA TCTACAAGAG CGCCTGCAAG
    ATTGAGGTGA AGTACTTTCC CTTCGACCAG CAGAACTGCA CCCTCAAGTT CCGCTCCTGG
    ACCTATGACC ACACGGAGAT AGACATGGTC CTCATGACGC CCACAGCCAG CATGGATGAC
    TTTACTCCCA GTGGTGAGTG GGACATAGTG GCCCTCCCAG GGAGAAGGAC AGTGAACCCA
    CAAGACCCCA GCTACGTGGA CGTGACTTAC GACTTCATCA TCAAGCGCAA GCCTCTGTTC
    TACACCATCA ACCTCATCAT CCCCTGCGTG CTCACCACCT TGCTGGCCAT CCTCGTCTTC
    TACCTGCCAT CCGACTGCGG CGAGAAGATG ACACTGTGCA TCTCAGTGCT GCTGGCACTG
    ACATTCTTCC TGCTGCTCAT CTCCAAGATC GTGCCACCCA CCTCCCTCGA TGTGCCTCTC
    ATCGGCAAGT ACCTCATGTT CACCATGGTG CTGGTCACCT TCTCCATCGT CACCAGCGTC
    TGTGTGCTCA ATGTGCACCA CCGCTCGCCC AGCACCCACA CCATGGCACC CTGGGTCAAG
    CGCTGCTTCC TGCACAAGCT GCCTACCTTC CTCTTCATGA AGCGCCCTGG CCCCGACAGC
    AGCCCGGCCA GAGCCTTCCC GCCCAGCAAG TCATGCGTGA CCAAGCCCGA GGCCACCGCC
    ACCTCCACCA GCCCCTCCAA CTTCTATGGG AACTCCATGT ACTTTGTGAA CCCCGCCTCT
    GCAGCTTCCA AGTCTCCAGC CGGCTCTACC CCGGTGGCTA TCCCCAGGGA TTTCTGGCTG
    CGGTCCTCTG GGAGGTTCCG ACAGGATGTG CAGGAGGCAT TAGAAGGTGT CAGCTTCATC
    GCCCAGCACA TGAAGAATGA CGATGAAGAC CAGAGTGTCG TTGAGGACTG GAAGTACGTG
    GCTATGGTGG TGGACCGGCT GTTCCTGTGG GTGTTCATGT TTGTGTGCGT CCTGGGCACT
    GTGGGGCTCT TCCTACCGCC CCTCTTCCAG ACCCATGCAG CTTCTGAGGG GCCCTACGCT
    GCCCAGCGTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56900
    Clone ID Related Accession (Same CDS sequence) XM_011521190.2 , XM_011521191.2
    Accession Version XM_011521191.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACCACCA ATGTCTGGCT GAAACAGGAA TGGACTGATT ACCGCCTGAC CTGGAACAGC 
    TCCCGCTACG AGGGTGTGAA CATCCTGAGG ATCCCTGCAA AGCGCATCTG GTTGCCTGAC
    ATCGTGCTTT ACAACAACGC CGACGGGACC TATGAGGTGT CTGTCTACAC CAACTTGATA
    GTCCGGTCCA ACGGCAGCGT CCTGTGGCTG CCCCCTGCCA TCTACAAGAG CGCCTGCAAG
    ATTGAGGTGA AGTACTTTCC CTTCGACCAG CAGAACTGCA CCCTCAAGTT CCGCTCCTGG
    ACCTATGACC ACACGGAGAT AGACATGGTC CTCATGACGC CCACAGCCAG CATGGATGAC
    TTTACTCCCA GTGGTGAGTG GGACATAGTG GCCCTCCCAG GGAGAAGGAC AGTGAACCCA
    CAAGACCCCA GCTACGTGGA CGTGACTTAC GACTTCATCA TCAAGCGCAA GCCTCTGTTC
    TACACCATCA ACCTCATCAT CCCCTGCGTG CTCACCACCT TGCTGGCCAT CCTCGTCTTC
    TACCTGCCAT CCGACTGCGG CGAGAAGATG ACACTGTGCA TCTCAGTGCT GCTGGCACTG
    ACATTCTTCC TGCTGCTCAT CTCCAAGATC GTGCCACCCA CCTCCCTCGA TGTGCCTCTC
    ATCGGCAAGT ACCTCATGTT CACCATGGTG CTGGTCACCT TCTCCATCGT CACCAGCGTC
    TGTGTGCTCA ATGTGCACCA CCGCTCGCCC AGCACCCACA CCATGGCACC CTGGGTCAAG
    CGCTGCTTCC TGCACAAGCT GCCTACCTTC CTCTTCATGA AGCGCCCTGG CCCCGACAGC
    AGCCCGGCCA GAGCCTTCCC GCCCAGCAAG TCATGCGTGA CCAAGCCCGA GGCCACCGCC
    ACCTCCACCA GCCCCTCCAA CTTCTATGGG AACTCCATGT ACTTTGTGAA CCCCGCCTCT
    GCAGCTTCCA AGTCTCCAGC CGGCTCTACC CCGGTGGCTA TCCCCAGGGA TTTCTGGCTG
    CGGTCCTCTG GGAGGTTCCG ACAGGATGTG CAGGAGGCAT TAGAAGGTGT CAGCTTCATC
    GCCCAGCACA TGAAGAATGA CGATGAAGAC CAGAGTGTCG TTGAGGACTG GAAGTACGTG
    GCTATGGTGG TGGACCGGCT GTTCCTGTGG GTGTTCATGT TTGTGTGCGT CCTGGGCACT
    GTGGGGCTCT TCCTACCGCC CCTCTTCCAG ACCCATGCAG CTTCTGAGGG GCCCTACGCT
    GCCCAGCGTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519493.1
    CDS83..1357
    Translation

    Target ORF information:

    RefSeq Version XM_011521191.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521191.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACCACCA ATGTCTGGCT GAAACAGGAA TGGACTGATT ACCGCCTGAC CTGGAACAGC 
    TCCCGCTACG AGGGTGTGAA CATCCTGAGG ATCCCTGCAA AGCGCATCTG GTTGCCTGAC
    ATCGTGCTTT ACAACAACGC CGACGGGACC TATGAGGTGT CTGTCTACAC CAACTTGATA
    GTCCGGTCCA ACGGCAGCGT CCTGTGGCTG CCCCCTGCCA TCTACAAGAG CGCCTGCAAG
    ATTGAGGTGA AGTACTTTCC CTTCGACCAG CAGAACTGCA CCCTCAAGTT CCGCTCCTGG
    ACCTATGACC ACACGGAGAT AGACATGGTC CTCATGACGC CCACAGCCAG CATGGATGAC
    TTTACTCCCA GTGGTGAGTG GGACATAGTG GCCCTCCCAG GGAGAAGGAC AGTGAACCCA
    CAAGACCCCA GCTACGTGGA CGTGACTTAC GACTTCATCA TCAAGCGCAA GCCTCTGTTC
    TACACCATCA ACCTCATCAT CCCCTGCGTG CTCACCACCT TGCTGGCCAT CCTCGTCTTC
    TACCTGCCAT CCGACTGCGG CGAGAAGATG ACACTGTGCA TCTCAGTGCT GCTGGCACTG
    ACATTCTTCC TGCTGCTCAT CTCCAAGATC GTGCCACCCA CCTCCCTCGA TGTGCCTCTC
    ATCGGCAAGT ACCTCATGTT CACCATGGTG CTGGTCACCT TCTCCATCGT CACCAGCGTC
    TGTGTGCTCA ATGTGCACCA CCGCTCGCCC AGCACCCACA CCATGGCACC CTGGGTCAAG
    CGCTGCTTCC TGCACAAGCT GCCTACCTTC CTCTTCATGA AGCGCCCTGG CCCCGACAGC
    AGCCCGGCCA GAGCCTTCCC GCCCAGCAAG TCATGCGTGA CCAAGCCCGA GGCCACCGCC
    ACCTCCACCA GCCCCTCCAA CTTCTATGGG AACTCCATGT ACTTTGTGAA CCCCGCCTCT
    GCAGCTTCCA AGTCTCCAGC CGGCTCTACC CCGGTGGCTA TCCCCAGGGA TTTCTGGCTG
    CGGTCCTCTG GGAGGTTCCG ACAGGATGTG CAGGAGGCAT TAGAAGGTGT CAGCTTCATC
    GCCCAGCACA TGAAGAATGA CGATGAAGAC CAGAGTGTCG TTGAGGACTG GAAGTACGTG
    GCTATGGTGG TGGACCGGCT GTTCCTGTGG GTGTTCATGT TTGTGTGCGT CCTGGGCACT
    GTGGGGCTCT TCCTACCGCC CCTCTTCCAG ACCCATGCAG CTTCTGAGGG GCCCTACGCT
    GCCCAGCGTG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56901
    Clone ID Related Accession (Same CDS sequence) XM_011521192.2
    Accession Version XM_011521192.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 951bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTCCTCA TGACGCCCAC AGCCAGCATG GATGACTTTA CTCCCAGTGG TGAGTGGGAC 
    ATAGTGGCCC TCCCAGGGAG AAGGACAGTG AACCCACAAG ACCCCAGCTA CGTGGACGTG
    ACTTACGACT TCATCATCAA GCGCAAGCCT CTGTTCTACA CCATCAACCT CATCATCCCC
    TGCGTGCTCA CCACCTTGCT GGCCATCCTC GTCTTCTACC TGCCATCCGA CTGCGGCGAG
    AAGATGACAC TGTGCATCTC AGTGCTGCTG GCACTGACAT TCTTCCTGCT GCTCATCTCC
    AAGATCGTGC CACCCACCTC CCTCGATGTG CCTCTCATCG GCAAGTACCT CATGTTCACC
    ATGGTGCTGG TCACCTTCTC CATCGTCACC AGCGTCTGTG TGCTCAATGT GCACCACCGC
    TCGCCCAGCA CCCACACCAT GGCACCCTGG GTCAAGCGCT GCTTCCTGCA CAAGCTGCCT
    ACCTTCCTCT TCATGAAGCG CCCTGGCCCC GACAGCAGCC CGGCCAGAGC CTTCCCGCCC
    AGCAAGTCAT GCGTGACCAA GCCCGAGGCC ACCGCCACCT CCACCAGCCC CTCCAACTTC
    TATGGGAACT CCATGTACTT TGTGAACCCC GCCTCTGCAG CTTCCAAGTC TCCAGCCGGC
    TCTACCCCGG TGGCTATCCC CAGGGATTTC TGGCTGCGGT CCTCTGGGAG GTTCCGACAG
    GATGTGCAGG AGGCATTAGA AGGTGTCAGC TTCATCGCCC AGCACATGAA GAATGACGAT
    GAAGACCAGA GTGTCGTTGA GGACTGGAAG TACGTGGCTA TGGTGGTGGA CCGGCTGTTC
    CTGTGGGTGT TCATGTTTGT GTGCGTCCTG GGCACTGTGG GGCTCTTCCT ACCGCCCCTC
    TTCCAGACCC ATGCAGCTTC TGAGGGGCCC TACGCTGCCC AGCGTGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519494.1
    CDS800..1750
    Translation

    Target ORF information:

    RefSeq Version XM_011521192.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521192.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTCCTCA TGACGCCCAC AGCCAGCATG GATGACTTTA CTCCCAGTGG TGAGTGGGAC 
    ATAGTGGCCC TCCCAGGGAG AAGGACAGTG AACCCACAAG ACCCCAGCTA CGTGGACGTG
    ACTTACGACT TCATCATCAA GCGCAAGCCT CTGTTCTACA CCATCAACCT CATCATCCCC
    TGCGTGCTCA CCACCTTGCT GGCCATCCTC GTCTTCTACC TGCCATCCGA CTGCGGCGAG
    AAGATGACAC TGTGCATCTC AGTGCTGCTG GCACTGACAT TCTTCCTGCT GCTCATCTCC
    AAGATCGTGC CACCCACCTC CCTCGATGTG CCTCTCATCG GCAAGTACCT CATGTTCACC
    ATGGTGCTGG TCACCTTCTC CATCGTCACC AGCGTCTGTG TGCTCAATGT GCACCACCGC
    TCGCCCAGCA CCCACACCAT GGCACCCTGG GTCAAGCGCT GCTTCCTGCA CAAGCTGCCT
    ACCTTCCTCT TCATGAAGCG CCCTGGCCCC GACAGCAGCC CGGCCAGAGC CTTCCCGCCC
    AGCAAGTCAT GCGTGACCAA GCCCGAGGCC ACCGCCACCT CCACCAGCCC CTCCAACTTC
    TATGGGAACT CCATGTACTT TGTGAACCCC GCCTCTGCAG CTTCCAAGTC TCCAGCCGGC
    TCTACCCCGG TGGCTATCCC CAGGGATTTC TGGCTGCGGT CCTCTGGGAG GTTCCGACAG
    GATGTGCAGG AGGCATTAGA AGGTGTCAGC TTCATCGCCC AGCACATGAA GAATGACGAT
    GAAGACCAGA GTGTCGTTGA GGACTGGAAG TACGTGGCTA TGGTGGTGGA CCGGCTGTTC
    CTGTGGGTGT TCATGTTTGT GTGCGTCCTG GGCACTGTGG GGCTCTTCCT ACCGCCCCTC
    TTCCAGACCC ATGCAGCTTC TGAGGGGCCC TACGCTGCCC AGCGTGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10996
    Clone ID Related Accession (Same CDS sequence) NM_001256567.2 , NM_001256567.1 , NM_001256567.3
    Accession Version NM_001256567.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC096081.1, AC067863.19, U62439.1, U48861.1 and AI625439.1. On Sep 2, 2017 this sequence version replaced NM_001256567.1. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2) with a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAG
    TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG TGGGTGTTCA
    TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC CAGACCCATG
    CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGAGG GCCCCCTGGG TTGTGGGGTG
    AGAGGATGTG AGTGGCCGGG TGGGCACTTT GCTGCTTCTT TCTGGGTTGT GGCTGATGAG
    GCCCTAAGTA AATATGTGAG CATTGGCCAT CAACCCCATC AAACCAGCCA CAGCCGTGGA
    ACAGGCAAGG ATGGGGGCCT GGGCTGTCCT CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243496.1
    CDS113..808
    Translation

    Target ORF information:

    RefSeq Version NM_001256567.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256567.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAG
    TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG TGGGTGTTCA
    TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC CAGACCCATG
    CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGAGG GCCCCCTGGG TTGTGGGGTG
    AGAGGATGTG AGTGGCCGGG TGGGCACTTT GCTGCTTCTT TCTGGGTTGT GGCTGATGAG
    GCCCTAAGTA AATATGTGAG CATTGGCCAT CAACCCCATC AAACCAGCCA CAGCCGTGGA
    ACAGGCAAGG ATGGGGGCCT GGGCTGTCCT CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10996
    Clone ID Related Accession (Same CDS sequence) NM_001256567.2 , NM_001256567.1 , NM_001256567.3
    Accession Version NM_001256567.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-22
    Organism Homo sapiens(Human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform 2 precursor
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC096081.1, AC067863.19, U62439.1 and U48861.1. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2) with a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAG
    TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG TGGGTGTTCA
    TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC CAGACCCATG
    CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGAGG GCCCCCTGGG TTGTGGGGTG
    AGAGGATGTG AGTGGCCGGG TGGGCACTTT GCTGCTTCTT TCTGGGTTGT GGCTGATGAG
    GCCCTAAGTA AATATGTGAG CATTGGCCAT CAACCCCATC AAACCAGCCA CAGCCGTGGA
    ACAGGCAAGG ATGGGGGCCT GGGCTGTCCT CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243496.1
    CDS113..808
    Translation

    Target ORF information:

    RefSeq Version NM_001256567.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAG
    TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG TGGGTGTTCA
    TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC CAGACCCATG
    CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGAGG GCCCCCTGGG TTGTGGGGTG
    AGAGGATGTG AGTGGCCGGG TGGGCACTTT GCTGCTTCTT TCTGGGTTGT GGCTGATGAG
    GCCCTAAGTA AATATGTGAG CATTGGCCAT CAACCCCATC AAACCAGCCA CAGCCGTGGA
    ACAGGCAAGG ATGGGGGCCT GGGCTGTCCT CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10996
    Clone ID Related Accession (Same CDS sequence) NM_001256567.2 , NM_001256567.1 , NM_001256567.3
    Accession Version NM_001256567.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-17
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC096081.1, AC067863.19, U62439.1, U48861.1 and AI625439.1. On May 31, 2019 this sequence version replaced NM_001256567.2. Transcript Variant: This variant (2) lacks an alternate exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2) with a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096083.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAG
    TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG TGGGTGTTCA
    TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC CAGACCCATG
    CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGAGG GCCCCCTGGG TTGTGGGGTG
    AGAGGATGTG AGTGGCCGGG TGGGCACTTT GCTGCTTCTT TCTGGGTTGT GGCTGATGAG
    GCCCTAAGTA AATATGTGAG CATTGGCCAT CAACCCCATC AAACCAGCCA CAGCCGTGGA
    ACAGGCAAGG ATGGGGGCCT GGGCTGTCCT CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243496.1
    CDS78..773
    Translation

    Target ORF information:

    RefSeq Version NM_001256567.3
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256567.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAG
    TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG TGGGTGTTCA
    TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC CAGACCCATG
    CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGAGG GCCCCCTGGG TTGTGGGGTG
    AGAGGATGTG AGTGGCCGGG TGGGCACTTT GCTGCTTCTT TCTGGGTTGT GGCTGATGAG
    GCCCTAAGTA AATATGTGAG CATTGGCCAT CAACCCCATC AAACCAGCCA CAGCCGTGGA
    ACAGGCAAGG ATGGGGGCCT GGGCTGTCCT CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56897
    Clone ID Related Accession (Same CDS sequence) XM_011521186.2 , XM_011521187.2
    Accession Version XM_011521186.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGCGGTA GTTTGCTGGC CCCAGCTCCA GATCATATTG TATTATGGAA CTGCCGCGTG 
    GCCAATGCGG AGGAAAAGCT GATGGACGAC CTTCTGAACA AAACCCGTTA CAATAACCTG
    ATCCGCCCAG CCACCAGCTC CTCACAGCTC ATCTCCATCA AGCTGCAGCT CTCCCTGGCC
    CAGCTTATCA GCGTGAATGA GCGAGAGCAG ATCATGACCA CCAATGTCTG GCTGAAACAG
    GAATGGACTG ATTACCGCCT GACCTGGAAC AGCTCCCGCT ACGAGGGTGT GAACATCCTG
    AGGATCCCTG CAAAGCGCAT CTGGTTGCCT GACATCGTGC TTTACAACAA CGCCGACGGG
    ACCTATGAGG TGTCTGTCTA CACCAACTTG ATAGTCCGGT CCAACGGCAG CGTCCTGTGG
    CTGCCCCCTG CCATCTACAA GAGCGCCTGC AAGATTGAGG TGAAGTACTT TCCCTTCGAC
    CAGCAGAACT GCACCCTCAA GTTCCGCTCC TGGACCTATG ACCACACGGA GATAGACATG
    GTCCTCATGA CGCCCACAGC CAGCATGGAT GACTTTACTC CCAGTGGTGA GTGGGACATA
    GTGGCCCTCC CAGGGAGAAG GACAGTGAAC CCACAAGACC CCAGCTACGT GGACGTGACT
    TACGACTTCA TCATCAAGCG CAAGCCTCTG TTCTACACCA TCAACCTCAT CATCCCCTGC
    GTGCTCACCA CCTTGCTGGC CATCCTCGTC TTCTACCTGC CATCCGACTG CGGCGAGAAG
    ATGACACTGT GCATCTCAGT GCTGCTGGCA CTGACATTCT TCCTGCTGCT CATCTCCAAG
    ATCGTGCCAC CCACCTCCCT CGATGTGCCT CTCATCGGCA AGTACCTCAT GTTCACCATG
    GTGCTGGTCA CCTTCTCCAT CGTCACCAGC GTCTGTGTGC TCAATGTGCA CCACCGCTCG
    CCCAGCACCC ACACCATGGC ACCCTGGGTC AAGCGCTGCT TCCTGCACAA GCTGCCTACC
    TTCCTCTTCA TGAAGCGCCC TGGCCCCGAC AGCAGCCCGG CCAGAGCCTT CCCGCCCAGC
    AAGTCATGCG TGACCAAGCC CGAGGCCACC GCCACCTCCA CCAGCCCCTC CAACTTCTAT
    GGGAACTCCA TGTACTTTGT GAACCCCGCC TCTGCAGCTT CCAAGTCTCC AGCCGGCTCT
    ACCCCGGTGG CTATCCCCAG GGATTTCTGG CTGCGGTCCT CTGGGAGGTT CCGACAGGAT
    GTGCAGGAGG CATTAGAAGG TGTCAGCTTC ATCGCCCAGC ACATGAAGAA TGACGATGAA
    GACCAGAGTG TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG
    TGGGTGTTCA TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC
    CAGACCCATG CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519488.1
    CDS152..1639
    Translation

    Target ORF information:

    RefSeq Version XM_011521186.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521186.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGCGGTA GTTTGCTGGC CCCAGCTCCA GATCATATTG TATTATGGAA CTGCCGCGTG 
    GCCAATGCGG AGGAAAAGCT GATGGACGAC CTTCTGAACA AAACCCGTTA CAATAACCTG
    ATCCGCCCAG CCACCAGCTC CTCACAGCTC ATCTCCATCA AGCTGCAGCT CTCCCTGGCC
    CAGCTTATCA GCGTGAATGA GCGAGAGCAG ATCATGACCA CCAATGTCTG GCTGAAACAG
    GAATGGACTG ATTACCGCCT GACCTGGAAC AGCTCCCGCT ACGAGGGTGT GAACATCCTG
    AGGATCCCTG CAAAGCGCAT CTGGTTGCCT GACATCGTGC TTTACAACAA CGCCGACGGG
    ACCTATGAGG TGTCTGTCTA CACCAACTTG ATAGTCCGGT CCAACGGCAG CGTCCTGTGG
    CTGCCCCCTG CCATCTACAA GAGCGCCTGC AAGATTGAGG TGAAGTACTT TCCCTTCGAC
    CAGCAGAACT GCACCCTCAA GTTCCGCTCC TGGACCTATG ACCACACGGA GATAGACATG
    GTCCTCATGA CGCCCACAGC CAGCATGGAT GACTTTACTC CCAGTGGTGA GTGGGACATA
    GTGGCCCTCC CAGGGAGAAG GACAGTGAAC CCACAAGACC CCAGCTACGT GGACGTGACT
    TACGACTTCA TCATCAAGCG CAAGCCTCTG TTCTACACCA TCAACCTCAT CATCCCCTGC
    GTGCTCACCA CCTTGCTGGC CATCCTCGTC TTCTACCTGC CATCCGACTG CGGCGAGAAG
    ATGACACTGT GCATCTCAGT GCTGCTGGCA CTGACATTCT TCCTGCTGCT CATCTCCAAG
    ATCGTGCCAC CCACCTCCCT CGATGTGCCT CTCATCGGCA AGTACCTCAT GTTCACCATG
    GTGCTGGTCA CCTTCTCCAT CGTCACCAGC GTCTGTGTGC TCAATGTGCA CCACCGCTCG
    CCCAGCACCC ACACCATGGC ACCCTGGGTC AAGCGCTGCT TCCTGCACAA GCTGCCTACC
    TTCCTCTTCA TGAAGCGCCC TGGCCCCGAC AGCAGCCCGG CCAGAGCCTT CCCGCCCAGC
    AAGTCATGCG TGACCAAGCC CGAGGCCACC GCCACCTCCA CCAGCCCCTC CAACTTCTAT
    GGGAACTCCA TGTACTTTGT GAACCCCGCC TCTGCAGCTT CCAAGTCTCC AGCCGGCTCT
    ACCCCGGTGG CTATCCCCAG GGATTTCTGG CTGCGGTCCT CTGGGAGGTT CCGACAGGAT
    GTGCAGGAGG CATTAGAAGG TGTCAGCTTC ATCGCCCAGC ACATGAAGAA TGACGATGAA
    GACCAGAGTG TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG
    TGGGTGTTCA TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC
    CAGACCCATG CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56897
    Clone ID Related Accession (Same CDS sequence) XM_011521186.2 , XM_011521187.2
    Accession Version XM_011521187.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGCGGTA GTTTGCTGGC CCCAGCTCCA GATCATATTG TATTATGGAA CTGCCGCGTG 
    GCCAATGCGG AGGAAAAGCT GATGGACGAC CTTCTGAACA AAACCCGTTA CAATAACCTG
    ATCCGCCCAG CCACCAGCTC CTCACAGCTC ATCTCCATCA AGCTGCAGCT CTCCCTGGCC
    CAGCTTATCA GCGTGAATGA GCGAGAGCAG ATCATGACCA CCAATGTCTG GCTGAAACAG
    GAATGGACTG ATTACCGCCT GACCTGGAAC AGCTCCCGCT ACGAGGGTGT GAACATCCTG
    AGGATCCCTG CAAAGCGCAT CTGGTTGCCT GACATCGTGC TTTACAACAA CGCCGACGGG
    ACCTATGAGG TGTCTGTCTA CACCAACTTG ATAGTCCGGT CCAACGGCAG CGTCCTGTGG
    CTGCCCCCTG CCATCTACAA GAGCGCCTGC AAGATTGAGG TGAAGTACTT TCCCTTCGAC
    CAGCAGAACT GCACCCTCAA GTTCCGCTCC TGGACCTATG ACCACACGGA GATAGACATG
    GTCCTCATGA CGCCCACAGC CAGCATGGAT GACTTTACTC CCAGTGGTGA GTGGGACATA
    GTGGCCCTCC CAGGGAGAAG GACAGTGAAC CCACAAGACC CCAGCTACGT GGACGTGACT
    TACGACTTCA TCATCAAGCG CAAGCCTCTG TTCTACACCA TCAACCTCAT CATCCCCTGC
    GTGCTCACCA CCTTGCTGGC CATCCTCGTC TTCTACCTGC CATCCGACTG CGGCGAGAAG
    ATGACACTGT GCATCTCAGT GCTGCTGGCA CTGACATTCT TCCTGCTGCT CATCTCCAAG
    ATCGTGCCAC CCACCTCCCT CGATGTGCCT CTCATCGGCA AGTACCTCAT GTTCACCATG
    GTGCTGGTCA CCTTCTCCAT CGTCACCAGC GTCTGTGTGC TCAATGTGCA CCACCGCTCG
    CCCAGCACCC ACACCATGGC ACCCTGGGTC AAGCGCTGCT TCCTGCACAA GCTGCCTACC
    TTCCTCTTCA TGAAGCGCCC TGGCCCCGAC AGCAGCCCGG CCAGAGCCTT CCCGCCCAGC
    AAGTCATGCG TGACCAAGCC CGAGGCCACC GCCACCTCCA CCAGCCCCTC CAACTTCTAT
    GGGAACTCCA TGTACTTTGT GAACCCCGCC TCTGCAGCTT CCAAGTCTCC AGCCGGCTCT
    ACCCCGGTGG CTATCCCCAG GGATTTCTGG CTGCGGTCCT CTGGGAGGTT CCGACAGGAT
    GTGCAGGAGG CATTAGAAGG TGTCAGCTTC ATCGCCCAGC ACATGAAGAA TGACGATGAA
    GACCAGAGTG TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG
    TGGGTGTTCA TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC
    CAGACCCATG CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519489.1
    CDS59..1546
    Translation

    Target ORF information:

    RefSeq Version XM_011521187.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521187.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGCGGTA GTTTGCTGGC CCCAGCTCCA GATCATATTG TATTATGGAA CTGCCGCGTG 
    GCCAATGCGG AGGAAAAGCT GATGGACGAC CTTCTGAACA AAACCCGTTA CAATAACCTG
    ATCCGCCCAG CCACCAGCTC CTCACAGCTC ATCTCCATCA AGCTGCAGCT CTCCCTGGCC
    CAGCTTATCA GCGTGAATGA GCGAGAGCAG ATCATGACCA CCAATGTCTG GCTGAAACAG
    GAATGGACTG ATTACCGCCT GACCTGGAAC AGCTCCCGCT ACGAGGGTGT GAACATCCTG
    AGGATCCCTG CAAAGCGCAT CTGGTTGCCT GACATCGTGC TTTACAACAA CGCCGACGGG
    ACCTATGAGG TGTCTGTCTA CACCAACTTG ATAGTCCGGT CCAACGGCAG CGTCCTGTGG
    CTGCCCCCTG CCATCTACAA GAGCGCCTGC AAGATTGAGG TGAAGTACTT TCCCTTCGAC
    CAGCAGAACT GCACCCTCAA GTTCCGCTCC TGGACCTATG ACCACACGGA GATAGACATG
    GTCCTCATGA CGCCCACAGC CAGCATGGAT GACTTTACTC CCAGTGGTGA GTGGGACATA
    GTGGCCCTCC CAGGGAGAAG GACAGTGAAC CCACAAGACC CCAGCTACGT GGACGTGACT
    TACGACTTCA TCATCAAGCG CAAGCCTCTG TTCTACACCA TCAACCTCAT CATCCCCTGC
    GTGCTCACCA CCTTGCTGGC CATCCTCGTC TTCTACCTGC CATCCGACTG CGGCGAGAAG
    ATGACACTGT GCATCTCAGT GCTGCTGGCA CTGACATTCT TCCTGCTGCT CATCTCCAAG
    ATCGTGCCAC CCACCTCCCT CGATGTGCCT CTCATCGGCA AGTACCTCAT GTTCACCATG
    GTGCTGGTCA CCTTCTCCAT CGTCACCAGC GTCTGTGTGC TCAATGTGCA CCACCGCTCG
    CCCAGCACCC ACACCATGGC ACCCTGGGTC AAGCGCTGCT TCCTGCACAA GCTGCCTACC
    TTCCTCTTCA TGAAGCGCCC TGGCCCCGAC AGCAGCCCGG CCAGAGCCTT CCCGCCCAGC
    AAGTCATGCG TGACCAAGCC CGAGGCCACC GCCACCTCCA CCAGCCCCTC CAACTTCTAT
    GGGAACTCCA TGTACTTTGT GAACCCCGCC TCTGCAGCTT CCAAGTCTCC AGCCGGCTCT
    ACCCCGGTGG CTATCCCCAG GGATTTCTGG CTGCGGTCCT CTGGGAGGTT CCGACAGGAT
    GTGCAGGAGG CATTAGAAGG TGTCAGCTTC ATCGCCCAGC ACATGAAGAA TGACGATGAA
    GACCAGAGTG TCGTTGAGGA CTGGAAGTAC GTGGCTATGG TGGTGGACCG GCTGTTCCTG
    TGGGTGTTCA TGTTTGTGTG CGTCCTGGGC ACTGTGGGGC TCTTCCTACC GCCCCTCTTC
    CAGACCCATG CAGCTTCTGA GGGGCCCTAC GCTGCCCAGC GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85239
    Clone ID Related Accession (Same CDS sequence) XM_017021887.1
    Accession Version XM_017021887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT GTCCCAGAAG CAAACGCCAA GATGGGGACT TGGGTGCAGG
    TGGTTTACTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877376.1
    CDS17..1408
    Translation

    Target ORF information:

    RefSeq Version XM_017021887.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT GTCCCAGAAG CAAACGCCAA GATGGGGACT TGGGTGCAGG
    TGGTTTACTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85240
    Clone ID Related Accession (Same CDS sequence) XM_017021888.1
    Accession Version XM_017021888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT AATGCCAGCC TGCCAGAACA AGCACCCTTG GTGCTGGAGA
    AAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877377.1
    CDS17..1405
    Translation

    Target ORF information:

    RefSeq Version XM_017021888.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTGT AATGCCAGCC TGCCAGAACA AGCACCCTTG GTGCTGGAGA
    AAGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85241
    Clone ID Related Accession (Same CDS sequence) XM_017021889.1 , XM_017021889.2
    Accession Version XM_017021889.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTAG CCTCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877378.1
    CDS17..1366
    Translation

    Target ORF information:

    RefSeq Version XM_017021889.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTAG CCTCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85241
    Clone ID Related Accession (Same CDS sequence) XM_017021889.1 , XM_017021889.2
    Accession Version XM_017021889.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product neuronal acetylcholine receptor subunit beta-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTAG CCTCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877378.1
    CDS17..1366
    Translation

    Target ORF information:

    RefSeq Version XM_017021889.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cholinergic receptor nicotinic beta 4 subunit (CHRNB4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021889.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGGCGCG CGCCTTCCCT GGTCCTTTTC TTCCTGGTCG CCCTTTGCGG GCGCGGGAAC 
    TGCCGCGTGG CCAATGCGGA GGAAAAGCTG ATGGACGACC TTCTGAACAA AACCCGTTAC
    AATAACCTGA TCCGCCCAGC CACCAGCTCC TCACAGCTCA TCTCCATCAA GCTGCAGCTC
    TCCCTGGCCC AGCTTATCAG CGTGAATGAG CGAGAGCAGA TCATGACCAC CAATGTCTGG
    CTGAAACAGG AATGGACTGA TTACCGCCTG ACCTGGAACA GCTCCCGCTA CGAGGGTGTG
    AACATCCTGA GGATCCCTGC AAAGCGCATC TGGTTGCCTG ACATCGTGCT TTACAACAAC
    GCCGACGGGA CCTATGAGGT GTCTGTCTAC ACCAACTTGA TAGTCCGGTC CAACGGCAGC
    GTCCTGTGGC TGCCCCCTGC CATCTACAAG AGCGCCTGCA AGATTGAGGT GAAGTACTTT
    CCCTTCGACC AGCAGAACTG CACCCTCAAG TTCCGCTCCT GGACCTATGA CCACACGGAG
    ATAGACATGG TCCTCATGAC GCCCACAGCC AGCATGGATG ACTTTACTCC CAGTGGTGAG
    TGGGACATAG TGGCCCTCCC AGGGAGAAGG ACAGTGAACC CACAAGACCC CAGCTACGTG
    GACGTGACTT ACGACTTCAT CATCAAGCGC AAGCCTCTGT TCTACACCAT CAACCTCATC
    ATCCCCTGCG TGCTCACCAC CTTGCTGGCC ATCCTCGTCT TCTACCTGCC ATCCGACTGC
    GGCGAGAAGA TGACACTGTG CATCTCAGTG CTGCTGGCAC TGACATTCTT CCTGCTGCTC
    ATCTCCAAGA TCGTGCCACC CACCTCCCTC GATGTGCCTC TCATCGGCAA GTACCTCATG
    TTCACCATGG TGCTGGTCAC CTTCTCCATC GTCACCAGCG TCTGTGTGCT CAATGTGCAC
    CACCGCTCGC CCAGCACCCA CACCATGGCA CCCTGGGTCA AGCGCTGCTT CCTGCACAAG
    CTGCCTACCT TCCTCTTCAT GAAGCGCCCT GGCCCCGACA GCAGCCCGGC CAGAGCCTTC
    CCGCCCAGCA AGTCATGCGT GACCAAGCCC GAGGCCACCG CCACCTCCAC CAGCCCCTCC
    AACTTCTATG GGAACTCCAT GTACTTTGTG AACCCCGCCT CTGCAGCTTC CAAGTCTCCA
    GCCGGCTCTA CCCCGGTGGC TATCCCCAGG GATTTCTGGC TGCGGTCCTC TGGGAGGTTC
    CGACAGGATG TGCAGGAGGC ATTAGAAGGT GTCAGCTTCA TCGCCCAGCA CATGAAGAAT
    GACGATGAAG ACCAGAGTAG CCTCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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